SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_014148860.1 NCBI__GCF_000968535.2:WP_014148860.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
70% identity, 99% coverage: 3:315/316 of query aligns to 4:315/323 of P0ABK5

query
sites
P0ABK5

I

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K42 (= K43) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
70% identity, 99% coverage: 3:315/316 of query aligns to 5:316/321 of 6z4nAAA

query
sites
6z4nAAA

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binding pyridoxal-5'-phosphate: K43 (= K43), N73 (= N73), V177 (= V177), G178 (= G178), T179 (= T179), G180 (= G180), T182 (= T182), G230 (= G229), S274 (= S273), P301 (= P300)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K43), T70 (= T70), G72 (= G72), N73 (= N73), T74 (= T74), Q144 (= Q144), F145 (= F145), Q229 (= Q228), G230 (= G229), I231 (= I230), A233 (= A232)

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
70% identity, 99% coverage: 3:315/316 of query aligns to 4:315/323 of P0A1E3

query
sites
P0A1E3

I

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F

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N72 (= N73) binding pyridoxal 5'-phosphate
S273 (= S273) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1d6sA Crystal structure of the k41a mutant of o-acetylserine sulfhydrylase complexed in external aldimine linkage with methionine (see paper)
69% identity, 99% coverage: 3:315/316 of query aligns to 3:314/322 of 1d6sA

query
sites
1d6sA

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K
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F

D

A

S

D

L

L

active site: A41 (≠ K43), G228 (= G229)
binding methionine: T68 (= T70), N69 (≠ S71), N71 (= N73), T72 (= T74), Q142 (= Q144), F143 (= F145), G176 (= G178), G228 (= G229)
binding pyridoxal-5'-phosphate: N71 (= N73), G176 (= G178), T177 (= T179), G178 (= G180), T180 (= T182), G228 (= G229), S272 (= S273), P299 (= P300)

1fcjA Crystal structure of oass complexed with chloride and sulfate (see paper)
70% identity, 95% coverage: 3:303/316 of query aligns to 3:302/302 of 1fcjA

query
sites
1fcjA

I

I

F

Y

D

E

D

D

N

N

S

S

L

L

S

T

I

I

G

G

R

H

T

T

P

P

L

L

V

V

K

R

L

L

N

N

R

R

I

I

A

G

G

N

N

G

K

R

A

-

T

-

V

I

L

L

A

A

K

K

I

V

E

E

G

S

R

R

N

N

P

P

A

S

Y

F

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

A

N

M

M

I

I

W

W

D

D

A

A

E

E

Q

K

R

R

G

G

I

V

L

L

K

K

P

P

G

G

I

V

E

E

I

L

V

V

E

E

P

P

T

T

S

N

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

S

K

L

L

T

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

I

I

E

E

R

R

K

K

V

L

L

L

A

K

A

A

F

L

G

G

A

A

Q

N

L

L

F

V

L

L

T

T

P

E

G

G

A

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

Q

Q

R

K

A

A

E

E

I

I

V

V

Q

A

S

S

D

D

P

P

D

Q

R

K

Y

Y

F

L

M

L

P

L

Q

Q

Q
|
Q

F

F

K

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

E

E

K

K

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

D

G

G

S

Q

V

V

D

D

V

V

L

F

V

I

S

S

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

S

T

R

R

Y

Y

I

I

K

K

Q

G

T

T

Q

K

G

G

K

K

-

T

N

D

I

L

L

I

S

T

V

V

A

A

V

V

E

E

P

P

K

T

E

D

S

S

P

P

V

V

I

I

S

A

Q

Q

K

A

L

L

A

A

G

G

Q

E

E

E

L

I

Q

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

D

G

T

N

L

L

D

D

L

L

S

K

V

L

V

I

D

D

R

K

I

V

E

V

Q

G

V

I

E

T

S

N

M

E

E

E

A

A

V

I

E

S

M

T

A

A

R

R

L

L

A

M

Q

E

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

C

S

G

G

A

A

M

V

V

A

A

A

I

L

R

K

L

L

A

Q

E

E

E

D

D

E

E

S

F

F

A

T

G

N

K

K

T

N

I

I

V

V

I

I

L

L

P
|
P

D

S

S

S

G

G

active site: K41 (= K43), G228 (= G229), S272 (= S273)
binding pyridoxal-5'-phosphate: K41 (= K43), N71 (= N73), V175 (= V177), G176 (= G178), T177 (= T179), G178 (= G180), T180 (= T182), G228 (= G229), S272 (= S273), P299 (= P300)
binding sulfate ion: G70 (= G72), T72 (= T74), Q142 (= Q144)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
69% identity, 100% coverage: 1:315/316 of query aligns to 2:309/310 of 5xoqA

query
sites
5xoqA

M

M

N

P

I

I

F

F

D

K

D

D

N

N

S

S

L

E

S

S

I

I

G

G

R

R

T

T

P

P

L

L

V

V

K

Q

L

I

N

N

R

R

I

L

-

T

A

A

G

G

N

L

K

S

A

S

T

R

V

V

L

L

A

A

K

K

I

I

E

E

G

G

R

R

N

N

P

P

A

A

Y

Y

S

S

V

V

K

K

C

C

R

R

I

I

G

G

A

A

M

M

I

I

W

W

D

D

A

A

E

E

Q

Q

R

S

G

G

I

K

L

L

K

K

P

P

G

G

I

M

E

H

I

V

V

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

F

V

V

A

C

A

A

R

R

G

G

Y

Y

S

K

L

L

T

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

I

I

E

E

R

R

K

M

V

M

L

L

A

K

A

S

F

F

G

G

A

A

Q

D

L

L

F

V

L

L

T

T

P

P

G

G

A

A

E

D

G

G

M
|
M

K

K

G

G

A

A

I

I

Q

S

R

K

A

A

E

E

I

L

V

A

Q

-

S

A

D

Q

P

P

D

G

R

-

Y

W

F

F

M

I

P

P

Q

Q

Q
|
Q

F

F

K

K

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

V

K

K

T

T

G

G

P

P

E

E

I

I

W

W

N

N

D

D

T

T

D

E

G

G

S

Q

V

V

D

D

V

V

L

F

V

V

S

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

S

A

R

R

Y

F

I

L

K

K

Q

H

T

E

Q

K

G

K

K

H

N

P

I

V

L

H

S

V

V

V

A

A

V

V

E

E

P

P

K

A

E

A

S

S

P

P

V

V

I

L

S

A

Q

G

K

G

L

P

A

A

G

G

Q
x
R

E

-

L

-

Q

-

P

-

G

-

P

-

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

I

V

P

P

D

D

T

T

L

F

D

D

L

R

S

S

V

V

D

D

R

E

I

I

E

L

Q

S

V

V

E

T

S

D

M

D

E

E

A

A

V

I

E

E

M

T

A

A

R

R

R

K

L

L

A

A

Q

M

E

E

G

G

I

I

L

S

C

C

G

G

I

I

S

S

C

C

G

G

A

A

M

M

V

A

A

G

A

A

I

L

R

K

L

V

A

A

E

A

E

R

D

P

E

E

F

F

A

A

G

G

K

K

T

T

I

I

V

V

V

T

I

V

L

L

P

P

D

D

S

A

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

V

A

L

L

F

F

D

E

S

N

L

L

binding : T72 (= T70), S73 (= S71), G74 (= G72), T76 (= T74), M123 (= M121), Q144 (= Q144), R218 (≠ Q218), H219 (= H225), Q222 (= Q228), G223 (= G229), A226 (= A232)

5dbhX Crystal structure of o-acetylserine sulfhydrylase from haemophilus influenzae in complex with reaction intermediate alpha-aminoacrylate
68% identity, 98% coverage: 3:313/316 of query aligns to 2:311/312 of 5dbhX

query
sites
5dbhX

I

I

F

Y

D

A

D

D

N

N

S

S

L

Y

S

S

I

I

G

G

R

N

T

T

P

P

L

L

V

V

K

R

L

L

N

K

R

H

I

F

A

-

G

G

N

H

K

N

A

G

T

N

V

V

L

V

A

V

K

K

I

I

E

E

G

G

R

R

N

N

P

P

A

S

Y

Y

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

A

N

M

M

I

V

W

W

D

Q

A

A

E

E

Q

K

R

D

G

G

I

T

L

L

K

T

P

K

G

G

I

K

E

E

I

I

V

V

E

D

P

A

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

S

K

L

I

T

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

I

L

E

E

R

R

R

K

K

R

V

L

L

L

A

C

A

G

F

L

G

G

A

V

Q

N

L

L

F

V

L

L

T

T

P

E

G

G

A

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

Q

A

R

K

A

A

E

E

I

I

V

V

Q

A

S

S

D

D

P

P

D

S

R

R

Y

Y

F

V

M

M

P

L

Q

K

Q
|
Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

K

D

D

T

T

D

D

G

G

S

K

V

V

D

D

V

V

L

V

V

V

S

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
x
S

I

I

T

T

G

G

V

I

S

S

R

R

Y

A

I

I

K

K

Q

L

T

D

Q

F

G

G

K

K

N

Q

I

I

L

T

S

S

V

V

A

A

V

V

E

E

P

P

K

V

E

E

S

S

P

P

V

V

I

I

S

S

Q

Q

K

T

L

L

A

A

G

G

Q

E

E

E

L

V

Q

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

D

K

T

N

L

L

D

D

L

L

S

S

V

I

D

D

R

R

I

V

E

E

Q

T

V

V

E

D

S

S

M

D

E

T

A

A

V

L

E

A

M

T

A

A

R

R

L

L

A

M

Q

A

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

C

S

G

G

A

A

M

V

V

A

A

A

I

D

R

R

L

L

A

A

E

K

E

L

D

P

E

E

F

F

A

A

G

D

K

K

T

L

I

I

V

V

I

I

L

L

P
|
P

D

S

S

A

G

S

E

E

R

R

Y

Y

L

L

S

S

S

T

V

A

L

L

F

F

D

E

binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: K41 (= K43), T68 (= T70), S69 (= S71), N71 (= N73), T72 (= T74), Q142 (= Q144), G176 (= G178), T177 (= T179), G178 (= G180), S180 (≠ T182), G227 (= G229), S271 (= S273), P298 (= P300)

1y7lA O-acetylserine sulfhydrylase complex (see paper)
68% identity, 98% coverage: 3:312/316 of query aligns to 2:310/310 of 1y7lA

query
sites
1y7lA

I

I

F

Y

D

A

D

D

N

N

S

S

L

Y

S

S

I

I

G

G

R

N

T

T

P

P

L

L

V

V

K

R

L

L

N

K

R

H

I

F

A

-

G

G

N

H

K

N

A

G

T

N

V

V

L

V

A

V

K

K

I

I

E

E

G

G

R

R

N

N

P

P

A

S

Y

Y

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

A

N

M

M

I

V

W

W

D

Q

A

A

E

E

Q

K

R

D

G

G

I

T

L

L

K

T

P

K

G

G

I

K

E

E

I

I

V

V

E

D

P

A

T
|
T

S
|
S

G

G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

S

K

L

I

T

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

I

L

E

E

R

R

R

K

K

R

V

L

L

L

A

C

A

G

F

L

G

G

A

V

Q

N

L

L

F

V

L

L

T

T

P

E

G

G

A

A

E

K

G

G

M
|
M

K

K

G

G

A

A

I

I

Q

A

R

K

A

A

E

E

I

I

V

V

Q

A

S

S

D

D

P

P

D

S

R

R

Y

Y

F

V

M

M

P

L

Q

K

Q
|
Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

K

D

D

T

T

D

D

G

G

S

K

V

V

D

D

V

V

L

V

V

V

S

A

G

G

V

V

G

G

T

T

G

G

T

S

I

I

T

T

G

G

V

I

S

S

R

R

Y

A

I

I

K

K

Q

L

T

D

Q

F

G

G

K

K

N

Q

I

I

L

T

S

S

V

V

A

A

V

V

E

E

P

P

K

V

E

E

S

S

P

P

V

V

I

I

S

S

Q

Q

K

T

L

L

A

A

G

G

Q

E

E

E

L

V

Q

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

D

K

T

N

L

L

D

D

L

L

S

S

V

I

D

D

R

R

I

V

E

E

Q

T

V

V

E

D

S

S

M

D

E

T

A

A

V

L

E

A

M

T

A

A

R

R

L

L

A

M

Q

A

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

C

S

G

G

A

A

M

V

V

A

A

A

I

D

R

R

L

L

A

A

E

K

E

L

D

P

E

E

F

F

A

A

G

D

K

K

T

L

I

I

V

V

I

I

L

L

P
|
P

D

S

S

A

G

S

E

E

R

R

Y

Y

L

L

S

S

S

T

V

A

L

L

F

F

active site: K41 (= K43), S271 (= S273), P298 (= P300)
binding : K41 (= K43), T68 (= T70), S69 (= S71), T72 (= T74), M119 (= M121), Q142 (= Q144)

4zu6X Crystal structure of o-acetylserine sulfhydrylase from haemophilus influenzae in complex with pre-reactive o-acetyl serine, alpha- aminoacrylate reaction intermediate and peptide inhibitor at the resolution of 2.25a
67% identity, 99% coverage: 1:312/316 of query aligns to 1:309/309 of 4zu6X

query
sites
4zu6X

M

M

N

A

I

I

F

Y

D

A

D

D

N

N

S

S

L

Y

S

S

I

I

G

G

R

N

T

T

P

P

L

L

V

V

K

R

L

L

N

K

R

H

I

F

A

-

G

G

N

H

K

N

A

G

T

N

V

V

L

V

A

V

K

K

I

I

E

E

G

G

R

R

N

N

P

P

A

S

Y

Y

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

A

N

M

M

I

V

W

W

D

Q

A

A

E

E

Q

K

R

D

G

G

I

T

L

L

K

T

P

K

G

G

I

K

E

E

I

I

V

V

E

D

P

A

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

S

K

L

I

T

T

L

L

T

T

M

M

P

P

D

E

T
|
T

M
|
M

S

S

I

L

E

E

R

R

R

K

K

R

V

L

L

L

A

C

A

G

F

L

G

G

A

V

Q

N

L

L

F

V

L

L

T

T

P

E

G

G

A

A

E
x
K

G
|
G

M

-

K

-

G

G

A

A

I

I

Q

A

R

K

A

A

E

E

I

I

V

V

Q

A

S

S

D

D

P

P

D

S

R

R

Y

Y

F

V

M

M

P

L

Q

K

Q
|
Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

K

D

D

T

T

D

D

G

G

S

K

V

V

D

D

V

V

L

V

V

V

S

A

G

G

V
|
V

G
|
G

T
|
T

G
|
G

T
x
S

I

I

T

T

G

G

V

I

S

S

R

R

Y

A

I

I

K

K

Q

L

T

D

Q

F

G

G

K

K

N

Q

I

I

L

T

S

S

V

V

A

A

V

V

E

E

P

P

K

V

E

E

S

S

P

P

V

V

I

I

S

S

Q

Q

K

T

L

L

A

A

G

G

Q

E

E

E

L

V

Q

K

P

P

G

G

P
|
P

H
|
H

K
|
K

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

I

I

P

P

D

K

T

N

L

L

D

D

L

L

S

S

V

I

D

D

R

R

I

V

E

E

Q

T

V

V

E

D

S

S

M

D

E

T

A

A

V

L

E

A

M

T

A

A

R

R

L

L

A

M

Q

A

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

C

S

G

G

A

A

M

V

V

A

A

A

I

D

R

R

L

L

A

A

E

K

E

L

D

P

E

E

F

F

A

A

G

D

K

K

T

L

I

I

V

V

I

I

L

L

P
|
P

D

S

S

A

G

S

E

E

R

R

Y

Y

L

L

S

S

S

T

V

A

L

L

F

F

binding o-acetylserine: T69 (= T70), S70 (= S71), T73 (= T74), Q141 (= Q144), G175 (= G178), G226 (= G229)
binding pyridoxal-5'-phosphate: K42 (= K43), N72 (= N73), V174 (= V177), G175 (= G178), T176 (= T179), G177 (= G180), G178 (= G181), S179 (≠ T182), G226 (= G229), S270 (= S273), P297 (= P300)
binding : S70 (= S71), T95 (= T96), M96 (= M97), K118 (≠ E119), G119 (= G120), P221 (= P224), H222 (= H225), K223 (= K226), A229 (= A232)

4oreX Cyrstal structure of o-acetylserine sulfhydrylase ternary complex from haemophilus influenzae at 2.2 a
67% identity, 98% coverage: 3:312/316 of query aligns to 2:308/308 of 4oreX

query
sites
4oreX

I

I

F

Y

D

A

D

D

N

N

S

S

L

Y

S

S

I

I

G

G

R

N

T

T

P

P

L

L

V

V

K

R

L

L

N

K

R

H

I

F

A

-

G

G

N

H

K

N

A

G

T

N

V

V

L

V

A

V

K

K

I

I

E

E

G

G

R

R

N

N

P

P

A

S

Y

Y

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

A

N

M

M

I

V

W

W

D

Q

A

A

E

E

Q

K

R

D

G

G

I

T

L

L

K

T

P

K

G

G

I

K

E

E

I

I

V

V

E

D

P

A

T
|
T

S

-

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

S

K

L

I

T

T

L

L

T

T

M

M

P
|
P

D

E

T

T

M

M

S

S

I

L

E

E

R

R

R

K

K

R

V

L

L

L

A

C

A

G

F

L

G

G

A

V

Q

N

L

L

F

V

L

L

T

T

P

E

G

-

A

A

E
x
K

G
|
G

M
|
M

K

K

G

G

A

A

I

I

Q

A

R

K

A

A

E

E

I

I

V

V

Q

A

S

S

D

D

P

P

D

S

R

R

Y

Y

F

V

M

M

P

L

Q

K

Q

Q

F
|
F

K

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

K

D

D

T

T

D

D

G

G

S

K

V

V

D

D

V

V

L

V

V

V

S

A

G

G

V

V

G

G

T

T

G

G

T

S

I

I

T

T

G

G

V

I

S

S

R

R

Y

A

I

I

K

K

Q

L

T

D

Q

F

G

G

K

K

N

Q

I

I

L

T

S

S

V

V

A

A

V

V

E

E

P

P

K

V

E

E

S

S

P

P

V

V

I

I

S

S

Q

Q

K

T

L

L

A

A

G

G

Q

E

E

E

L

V

Q

K

P

P

G
|
G

P
|
P

H
|
H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

I

I

P

P

D

K

T

N

L

L

D

D

L

L

S

S

V

I

D

D

R

R

I

V

E

E

Q

T

V

V

E

D

S

S

M

D

E

T

A

A

V

L

E

A

M

T

A

A

R

R

L

L

A

M

Q

A

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S

S

C

S

G

G

A

A

M

V

V

A

A

A

I

D

R

R

L

L

A

A

E

K

E

L

D

P

E

E

F

F

A

A

G

D

K

K

T

L

I

I

V

V

I

I

L

L

P

P

D

S

S

A

G

S

E

E

R

R

Y

Y

L

L

S

S

S

T

V

A

L

L

F

F

binding o-acetylserine: K115 (≠ E119), G116 (= G120), M117 (= M121)
binding : K41 (= K43), T68 (= T70), G69 (= G72), T71 (= T74), P91 (= P94), M117 (= M121), F141 (= F145), G219 (= G223), P220 (= P224), H221 (= H225), G225 (= G229), A228 (= A232)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
60% identity, 99% coverage: 1:312/316 of query aligns to 1:305/310 of P9WP55

query
sites
P9WP55

M

M

N

S

I

I

F

A

D

E

D

D

N

I

S

T

L

Q

S

L

I

I

G

G

R

R

T

T

P

P

L

L

V

V

K

R

L

L

N

R

R

R

I

V

A

T

-

D

G

G

N

A

K

V

A

A

T

D

V

I

L

V

A

A

K

K

I

L

E

E

G

F

R

F

N

N

P

P

A

A

Y

N

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

V

A

A

M

M

I

L

W

Q

D

A

A

A

E

E

Q

Q

R

A

G

G

I

L

L

I

K

K

P

P

G

D

I

T

E

I

I

I

V

L

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

Y

M

V

V

A

C

A

A

R

R

G

G

Y

Y

S

R

L

C

T

V

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

I

L

E

E

R

R

K

M

V

L

L

L

A

R

A

A

F

Y

G

G

A

A

Q

E

L

L

F

I

L

L

T

T

P

P

G

G

A

A

E

D

G

G

M

M

K

S

G

G

A

A

I

I

Q

A

R

K

A

A

E

E

I

L

V

A

Q

K

S

T

D

D

P

-

D

Q

R

R

Y

Y

F

F

M

V

P

P

Q

Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

R

K

V

T

T

G

A

P

E

E

E

I

V

W

W

N

R

D

D

T

T

D

D

G

G

S

K

V

V

D

D

V

I

L

V

V

V

S

A

G

G

V

V

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

I

I

K

K

Q

E

T

R

Q

K

G

-

K

P

N

S

I

A

L

R

S

F

V

V

A

A

V

V

E

E

P

P

K

A

E

A

S

S

P

P

V

V

I

L

S

S

Q

-

K

-

L

-

A

G

G

G

Q

Q

E

K

L

-

Q

-

P

-

G

G

P

P

H

H

K

P

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

V

P

P

D

P

T

V

L

L

D

D

L

Q

S

D

V

L

V

V

D

D

R

E

I

I

E

I

Q

T

V

V

E

G

S

N

M

E

E

D

A

A

V

L

E

N

M

V

A

A

R

R

L

L

A

A

Q

R

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S
|
S

C

S

G

G

A

A

M

T

V

V

A

A

I

L

R

Q

L

V

A

A

E

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

I

V

V

I

V

L

L

P

P

D

D

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

V

P

L

L

F

F

K44 (= K43) modified: N6-(pyridoxal phosphate)lysine
N74 (= N73) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 178:182) binding pyridoxal 5'-phosphate
S266 (= S273) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
60% identity, 99% coverage: 1:312/316 of query aligns to 1:305/306 of 2q3dA

query
sites
2q3dA

M

M

N

S

I

I

F

A

D

E

D

D

N

I

S

T

L

Q

S

L

I

I

G

G

R

R

T

T

P

P

L

L

V

V

K

R

L

L

N

R

R

R

I

V

A

T

-

D

G

G

N

A

K

V

A

A

T

D

V

I

L

V

A

A

K

K

I

L

E

E

G

F

R

F

N

N

P

P

A

A

Y

N

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

V

A

A

M

M

I

L

W

Q

D

A

A

A

E

E

Q

Q

R

A

G

G

I

L

L

I

K

K

P

P

G

D

I

T

E

I

I

I

V

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

M

V

V

A

C

A

A

R

R

G

G

Y

Y

S

R

L

C

T

V

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

I

L

E

E

R

R

K

M

V

L

L

L

A

R

A

A

F

Y

G

G

A

A

Q

E

L

L

F

I

L

L

T

T

P

P

G

G

A

A

E

D

G

G

M

M

K

S

G

G

A

A

I

I

Q

A

R

K

A

A

E

E

I

L

V

A

Q

K

S

T

D

D

P

-

D

Q

R

R

Y

Y

F

F

M

V

P

P

Q

Q

Q
|
Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

R

K

V

T

T

G

A

P

E

E

E

I

V

W

W

N

R

D

D

T

T

D

D

G

G

S

K

V

V

D

D

V

I

L

V

V

V

S

A

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

I

I

K

K

Q

E

T

R

Q

K

G

-

K

P

N

S

I

A

L

R

S

F

V

V

A

A

V

V

E

E

P

P

K

A

E

A

S

S

P

P

V

V

I

L

S

S

Q

-

K

-

L

-

A

G

G

G

Q

Q

E

K

L

-

Q

-

P

-

G

G

P

P

H

H

K

P

I

I

Q

Q

G
|
G

I
|
I

G

G

A

A

G

G

F

F

I

V

P

P

D

P

T

V

L

L

D

D

L

Q

S

D

V

L

V

V

D

D

R

E

I

I

E

I

Q

T

V

V

E

G

S

N

M

E

E

D

A

A

V

L

E

N

M

V

A

A

R

R

L

L

A

A

Q

R

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S
|
S

C

S

G

G

A

A

M

T

V

V

A

A

I

L

R

Q

L

V

A

A

E

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

I

V

V

I

V

L

L

P
|
P

D
|
D

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

V

P

L

L

F

F

active site: K44 (= K43), S266 (= S273), P293 (= P300)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K43), T71 (= T70), S72 (= S71), N74 (= N73), T75 (= T74), Q144 (= Q144), V177 (= V177), G178 (= G178), T179 (= T179), G180 (= G180), T182 (= T182), G222 (= G229), I223 (= I230), S266 (= S273), P293 (= P300), D294 (= D301)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
60% identity, 97% coverage: 1:307/316 of query aligns to 1:300/300 of 3zeiA

query
sites
3zeiA

M

M

N

S

I

I

F

A

D

E

D

D

N

I

S

T

L

Q

S

L

I

I

G

G

R

R

T

T

P

P

L

L

V

V

K

R

L

L

N

R

R

R

I

V

A

T

-

D

G

G

N

A

K

V

A

A

T

D

V

I

L

V

A

A

K

K

I

L

E

E

G

F

R

F

N

N

P

P

A

A

Y

N

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

V

A

A

M

M

I

L

W

Q

D

A

A

A

E

E

Q

Q

R

A

G

G

I

L

L

I

K

K

P

P

G

D

I

T

E

I

I

I

V

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

Y

M

V

V

A

C

A

A

R

R

G

G

Y

Y

S

R

L

C

T

V

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

I

L

E

E

R

R

K

M

V

L

L

L

A

R

A

A

F

Y

G

G

A

A

Q

E

L

L

F

I

L

L

T

T

P

P

G

G

A

A

E

D

G

G

M

M

K

S

G

G

A

A

I
|
I

Q

A

R

K

A

A

E

E

I

L

V

A

Q

K

S

T

D

D

P

-

D

Q

R

R

Y

Y

F

F

M

V

P

P

Q

Q

Q
|
Q

F
|
F

K

E

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

R

K

V

T

T

G

A

P

E

E

E

I

V

W

W

N

R

D

D

T

T

D

D

G

G

S

K

V

V

D

D

V

I

L

V

V

V

S

A

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

I

I

K

K

Q

E

T

R

Q

K

G

-

K

P

N

S

I

A

L

R

S

F

V

V

A

A

V

V

E

E

P

P

K

A

E

A

S

S

P

P

V

V

I

L

S

S

Q

-

K

-

L

-

A

G

G

G

Q

Q

E
x
K

L

-

Q

-

P

-

G

G

P

P

H

H

K

P

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F
|
F

I

V

P

P

D

P

T

V

L

L

D

D

L

Q

S

D

V

L

V

V

D

D

R

E

I

I

E

I

Q

T

V

V

E

G

S

N

M

E

E

D

A

A

V

L

E

N

M

V

A

A

R

R

L

L

A

A

Q

R

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S
|
S

C

S

G

G

A

A

M

T

V

V

A

A

I

L

R

Q

L

V

A

A

E

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

I

V

V

I

V

L

L

P
|
P

D
|
D

S

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K43), S266 (= S273), P293 (= P300)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T70), S72 (= S71), I126 (= I125), Q144 (= Q144), F145 (= F145), K215 (≠ E219), G222 (= G229), A225 (= A232), F227 (= F234)
binding pyridoxal-5'-phosphate: K44 (= K43), N74 (= N73), V177 (= V177), G178 (= G178), T179 (= T179), G180 (= G180), T182 (= T182), G222 (= G229), S266 (= S273), P293 (= P300), D294 (= D301)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
60% identity, 97% coverage: 1:307/316 of query aligns to 1:300/300 of 2q3cA

query
sites
2q3cA

M

M

N

S

I

I

F

A

D

E

D

D

N

I

S

T

L

Q

S

L

I

I

G

G

R

R

T

T

P

P

L

L

V

V

K

R

L

L

N

R

R

R

I

V

A

T

-

D

G

G

N

A

K

V

A

A

T

D

V

I

L

V

A

A

K

K

I

L

E

E

G

F

R

F

N

N

P

P

A

A

Y

N

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

V

A

A

M

M

I

L

W

Q

D

A

A

A

E

E

Q

Q

R

A

G

G

I

L

L

I

K

K

P

P

G

D

I

T

E

I

I

I

V

L

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

M

V

V

A

C

A

A

R

R

G

G

Y

Y

S

R

L

C

T

V

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

I

L

E

E

R

R

K

M

V

L

L

L

A

R

A

A

F

Y

G

G

A

A

Q

E

L

L

F

I

L

L

T

T

P

P

G

G

A

A

E

D

G

G

M
|
M

K

S

G

G

A

A

I

I

Q

A

R

K

A

A

E

E

I

L

V

A

Q

K

S

T

D

D

P

-

D

Q

R

R

Y

Y

F

F

M

V

P

P

Q

Q

Q
|
Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

R

K

V

T

T

G

A

P

E

E

E

I

V

W

W

N

R

D

D

T

T

D

D

G

G

S

K

V

V

D

D

V

I

L

V

V

V

S

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

I

I

K

K

Q

E

T

R

Q

K

G

-

K

P

N

S

I

A

L

R

S

F

V

V

A

A

V

V

E

E

P

P

K

A

E

A

S

S

P

P

V

V

I

L

S

S

Q

-

K

-

L

-

A

G

G

G

Q

Q

E
x
K

L

-

Q

-

P

-

G

G

P

P

H

H

K

P

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

I

V

P

P

D

P

T

V

L

L

D

D

L

Q

S

D

V

L

V

V

D

D

R

E

I

I

E

I

Q

T

V

V

E

G

S

N

M

E

E

D

A

A

V

L

E

N

M

V

A

A

R

R

L

L

A

A

Q

R

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S
|
S

C

S

G

G

A

A

M

T

V

V

A

A

I

L

R

Q

L

V

A

A

E

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

I

V

V

I

V

L

L

P
|
P

D

D

S

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K43), S266 (= S273), P293 (= P300)
binding : T71 (= T70), S72 (= S71), G73 (= G72), T75 (= T74), M122 (= M121), Q144 (= Q144), K215 (≠ E219), G222 (= G229), A225 (= A232)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
56% identity, 100% coverage: 1:316/316 of query aligns to 11:320/323 of 4aecA

query
sites
4aecA

M

L

N

N

I

I

F

A

D

D

D

N

N

V

S

S

L

Q

S

L

I

I

G

G

R

K

T

T

P

P

L

M

V

V

K

Y

L

L

N

N

R

S

I

I

A

A

-

K

G

G

N

C

K

V

A

A

T

N

V

I

L

A

A

A

K

K

I

L

E

E

G

I

R

M

N

E

P

P

A

C

Y

C

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

Y

A

S

M

M

I

V

W

T

D

D

A

A

E

E

Q

Q

R

K

G

G

I

F

L

I

K

S

P

P

G

G

I

K

E

S

I

V

-

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

Y

F

V

I

A

A

A

S

R

R

G

G

Y

Y

S

R

L

L

T

I

L

L

T

T

M

M

P

P

D

A

T

S

M

M

S

S

I

M

E

E

R

R

K

V

V

L

L

L

A

K

A

A

F

F

G

G

A

A

Q

E

L

L

F

V

L

L

T

T

P

D

G

P

A

A

E

K

G

G

M

M

K

T

G

G

A

A

I

V

Q

Q

R

K

A

A

E

E

I

I

V

L

Q

K

S

N

D

T

P

P

D

D

R

A

Y

Y

F

M

M

L

P

-

Q

Q

F

F

K

D

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

D

D

D

T

T

D

K

G

G

S

K

V

V

D

D

V

I

L

F

V

V

S

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

V

S

G

R

R

Y

F

I

I

K

K

Q

E

T

K

Q

N

G

P

K

K

N

T

I

Q

L

V

S

-

V

I

A

G

V

V

E

E

P

P

K

T

E

E

S

S

P

D

V

I

I

L

S

S

Q

G

K

G

L

-

A

-

G

-

Q

-

E

-

L

-

Q

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

D

K

T

N

L

L

D

D

L

Q

S

K

V

I

V

M

D

D

R

E

I

V

E

I

Q

A

V

I

E

S

S

S

M

E

E

E

A

A

V

I

E

E

M

T

A

A

R

K

R

Q

L

L

A

A

Q

L

E

K

E

E

G

G

I

L

L

M

C

V

G

G

I

I

S
|
S

C

S

G

G

A

A

M

A

V

A

A

A

I

I

R

K

L

V

A

A

E

K

E

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

I

V

A

V

V

I

V

L

F

P
|
P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

V

P

L

L

F

F

D

Q

S

S

L

I

H

R

active site: K54 (= K43), S277 (= S273)
binding pyridoxal-5'-phosphate: K54 (= K43), N85 (= N73), I188 (≠ V177), G189 (= G178), T190 (= T179), G191 (= G180), G192 (= G181), T193 (= T182), G233 (= G229), S277 (= S273), P304 (= P300)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
56% identity, 100% coverage: 1:316/316 of query aligns to 1:312/318 of 4lmaA

query
sites
4lmaA

M

M

N

R

I

I

F

A

D

H

D

D

N

V

S

T

L

E

S

L

I

V

G

G

R

R

T

T

P

P

L

L

V

V

K

Q

L

L

N

N

R

R

I

I

-

P

-

Q

-

A

A

E

G

G

N

C

K

L

A

G

T

R

V

I

L

V

A

M

K

K

I

L

E

E

G

G

R

M

N

N

P

P

A

A

Y

A

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

T

A

H

M

M

I

I

W

N

D

S

A

A

E

E

Q

K

R

A

G

G

I

L

L

I

K

N

P

P

G

E

I

T

E

T

I

V

-

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

Y

M

V

T

A

A

R

K

G

G

Y

Y

S

R

L

L

T

I

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

I

L

E

E

R

R

K

A

V

M

L

L

A

K

A

A

F

Y

G

G

A

A

Q

T

L

L

F

E

L

L

T

T

P

P

G

G

A

S

E

Q

G

G

M

M

K

K

G

G

A

A

I

I

Q

L

R

R

A

A

E

Q

I

I

V

V

Q

D

S

S

D

I

P

P

D

G

R

A

Y

Y

F

M

M

L

P

-

Q

Q

F

F

K

R

N

N

P

P

A

S

N

N

P

P

A

E

I

I

H

H

E

R

K

L

T

T

G

A

P

E

E

E

I

I

W

W

N

Q

D

D

T

T

D

E

G

G

S

Q

V

V

D

D

V

F

L

I

V

V

S

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

E

Y

V

I

I

K

K

Q

-

T

S

Q

R

G

K

K

P

N

S

I

F

L

Q

S

V

V

V

A

A

V

V

E

E

P

P

K

F

E

N

S

S

P

P

V

V

I

I

S

S

Q

G

K

G

L

-

A

-

G

-

Q

-

E

-

L

-

Q

N

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

D

E

T

V

L

L

D

R

L

T

S

D

V

L

V

I

D

D

R

E

I

V

E

I

Q

T

V

V

E

S

S

D

M

E

E

E

A

A

V

F

E

Q

M

F

A

G

R

R

L

L

A

A

Q

K

E

E

G

G

I

L

L

L

C

S

G

G

I

I

S
|
S

C

S

G

G

A

A

A

N

M

L

V

C

A

A

I

I

R

Q

L

L

A

A

E

Q

E

R

D

P

E

E

F

N

A

E

G

G

K

K

T

L

I

I

V

V

I

I

L

Q

P
|
P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

V

L

L

M

F

F

D

Q

S

N

L

I

H

E

active site: K46 (= K43), S269 (= S273)
binding pyridoxal-5'-phosphate: K46 (= K43), N77 (= N73), G181 (= G178), T182 (= T179), G183 (= G180), T185 (= T182), G225 (= G229), S269 (= S273), P296 (= P300)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
54% identity, 100% coverage: 1:316/316 of query aligns to 73:382/392 of P47999

query
sites
P47999

M

L

N

N

I

I

F

A

D

D

D

N

N

A

S

A

L

Q

S

L

I

I

G

G

R

K

T

T

P

P

L

M

V

V

K

Y

L

L

N

N

R

N

I

V

A

V

-

K

G

G

N

C

K

V

A

A

T

S

V

V

L

A

A

A

K

K

I

L

E

E

G

I

R

M

N

E

P

P

A

C

Y

C

S

S

V

V

K

K

C

D

R

R

I

I

G

G

A

Y

A

S

M

M

I

I

W

T

D

D

A

A

E

E

Q

E

R

K

G

G

I

L

L

I

K

T

P

P

G

G

I

K

E

S

I

V

-

L

V

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

Y

F

V

I

A

A

A

S

R

K

G

G

Y

Y

S

K

L

L

T

I

L

L

T

T

M

M

P

P

D

A

T

S

M

M

S

S

I

L

E

E

R

R

K

V

V

L

L

L

A

R

A

A

F

F

G

G

A

A

Q

E

L

L

F

V

L

L

T

T

P

E

G

P

A

A

E

K

G

G

M

M

K

T

G

G

A

A

I

I

Q

Q

R

K

A

A

E

E

I

I

V

L

Q

K

S

K

D

T

P

P

D

N

R

S

Y

Y

F

M

M

L

P

-

Q

Q

F

F

K

D

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

R

G

G

S

K

V

I

D

D

V

I

L

L

V

V

S

A

G

G

V

I

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

S

G

R

R

Y

F

I

I

K

K

Q

E

T

R

Q

K

G

-

K

P

N

E

I

L

L

K

S

V

V

I

A

G

V

V

E

E

P

P

K

T

E

E

S

S

P

A

V

I

I

L

S

S

Q

G

K

G

L

-

A

-

G

-

Q

-

E

-

L

-

Q

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

V

P

P

D

K

T

N

L

L

D

D

L

L

S

A

V

I

V

V

D

D

R

E

I

Y

E

I

Q

A

V

I

E

S

S

S

M

E

E

E

A

A

V

I

E

E

M

T

A

S

R

K

R

Q

L

L

A

A

Q

L

E

Q

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S

S

C

S

G

G

A

A

M

A

V

A

A

A

I

I

R

Q

L

V

A

A

E

K

E

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

I

V

A

V

V

I

V

L

F

P

P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

V

Q

L

L

F

F

D

Q

S

S

L

I

H

R

Sites not aligning to the query:

61 modified: N-acetylalanine

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
57% identity, 99% coverage: 1:312/316 of query aligns to 1:308/310 of 4lmbA

query
sites
4lmbA

M

M

N

L

I

I

F

A

D

R

D

D

N

I

S

T

L

Q

S

L

I

V

G

G

R

R

T

T

P

P

L

L

V

V

K

Q

L

L

N

N

R

R

I

I

A

P

-

V

-

A

-

E

G

G

N

V

K

K

A

A

T

R

V

I

L

V

A

V

K

K

I

L

E

E

G

S

R

M

N

N

P

P

A

A

Y

A

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

V

A

S

M

M

I

V

W

E

D

D

A

A

E

E

Q

A

R

A

G

G

I

L

L

I

K

H

P

P

G

D

I

K

E

T

I

I

-

L

V

V

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

M

V

V

A

A

R

K

G

G

Y

Y

S

R

L

L

T

V

L

L

T

T

M

M

P

P

D

E

T

T

M
|
M

S

S

I

L

E

E

R

R

K

A

V

M

L

L

A

K

A

A

F

Y

G

G

A

A

Q

Q

L

L

F

E

L

L

T

T

P

P

G

G

A

S

E

Q

G

G

M
|
M

K

E

G

G

A

A

I

I

Q

T

R

R

A

A

E

E

I

I

V

V

Q

E

S

N

D

T

P

P

D

N

R

A

Y

Y

F

S

M

L

P

-

Q

Q

Q
|
Q

F
|
F

K

R

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

R

K

E

T

T

G

A

P

E

E

E

I

I

W

W

N

A

D

D

T

T

D

D

G

G

S

L

V

V

D

D

V

I

L

V

V

I

S

G

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

I

S

A

R

E

Y

T

I

I

K

K

Q

P

T

R

Q

R

G

P

K

Q

N

-

I

F

L

Q

S

A

V

I

A

A

V

V

E

E

P

P

K

S

E

N

S

S

P

P

V

V

I

L

S

S

Q

G

K

G

L

-

A

-

G

-

Q

-

E

-

L

-

Q

Q

P

P

G

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

I

I

P

P

D

A

T

I

L

F

D

R

L

P

S

E

V

L

V

I

D

D

R

E

I

V

E

I

Q

I

V

V

E

D

S

D

M

T

E

E

A

A

V

F

E

A

M

Y

A

A

R

R

L

L

A

A

Q

R

E

Q

E

E

G

G

I

L

L

L

C

S

G

G

I

I

S
|
S

C

A

G

G

A

A

M

L

V

W

A

A

I

I

R

Q

L

V

A

G

E

K

E

R

D

P

E

E

F

N

A

E

G

D

K

K

T

L

I

I

V

V

V

M

I

I

L

Q

P
|
P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

V

A

L

L

F

F

active site: K46 (= K43), S269 (= S273)
binding cysteine: K46 (= K43), T74 (= T70), S75 (= S71), N77 (= N73), T78 (= T74), M101 (= M97), M125 (= M121), M125 (= M121), Q147 (= Q144), F148 (= F145), Q224 (= Q228), G225 (= G229), G225 (= G229), I226 (= I230), A228 (= A232)
binding pyridoxal-5'-phosphate: K46 (= K43), N77 (= N73), V180 (= V177), G181 (= G178), T182 (= T179), G183 (= G180), T185 (= T182), G225 (= G229), S269 (= S273), P296 (= P300)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
56% identity, 99% coverage: 3:314/316 of query aligns to 5:310/322 of P47998

query
sites
P47998

I

I

F

A

D

K

D

D

N

V

S

T

L

E

S

L

I

I

G

G

R

N

T

T

P

P

L

L

V

V

K

Y

L

L

N

N

R

N

I

V

A

A

-

E

G

G

N

C

K

V

A

G

T

R

V

V

L

A

A

A

K

K

I

L

E

E

G

M

R

M

N

E

P

P

A

C

Y

S

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

F

A

S

M

M

I

I

W

S

D

D

A

A

E

E

Q

K

R

K

G

G

I

L

L

I

K

K

P

P

G

G

I

E

E

S

I

V

-

L

V

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

Y

F

V

T

A

A

R

K

G

G

Y

Y

S

K

L

L

T

I

L

I

T

T

M

M

P

P

D

A

T

S

M

M

S

S

I

T

E

E

R

R

K

I

V

I

L

L

A

L

A

A

F

F

G

G

A

V

Q

E

L

L

F

V

L

L

T

T

P

D

G

P

A

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

Q

A

R

K

A

A

E

E

I

I

V

L

Q

A

S

K

D

T

P

P

D

N

R

G

Y

Y

F

-

M

M

P

L

Q

Q

Q
|
Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H
|
H

E

Y

K

E

T

T

G
|
G

P

P

E

E

I

I

W

W

N

K

D

G

T

T

D

G

G

G

S

K

V

I

D

D

V

G

L

F

V

V

S

S

G

G

V

I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

Y

Y

I

L

K

K

Q

E

T

-

Q

Q

G

N

K

A

N

N

I

V

L

K

S

L

V

Y

A

G

V

V

E

E

P

P

K

V

E

E

S

S

P

A

V

I

I

L

S

S

Q

G

K

G

L

-

A

-

G

-

Q

-

E

-

L

-

Q
x
K

P

P

G

G

P

P

H
|
H

K
|
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

D

S

T

V

L

L

D

N

L

V

S

D

V

L

V

I

D

D

R

E

I

V

E

V

Q

Q

V

V

E

S

S

S

M

D

E

E

A

S

V

I

E

D

M

M

A

A

R

R

R

Q

L

L

A

A

Q

L

E

K

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S
|
S

C

S

G

G

A

A

M

A

V

A

A

A

I

I

R

K

L

L

A

A

E

Q

E

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

F

V

V

V

A

I

I

L

F

P

P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

V

V

L

L

F

F

D

D

S

A

K46 (= K43) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T70) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S71) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N73) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T74) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q144) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H154) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G159) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 178:182) binding pyridoxal 5'-phosphate
T182 (= T179) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T182) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ Q221) mutation to A: Impaired interaction with SAT1.
H221 (= H225) mutation to A: Impaired interaction with SAT1.
K222 (= K226) mutation to A: Impaired interaction with SAT1.
S269 (= S273) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
56% identity, 99% coverage: 3:314/316 of query aligns to 3:308/320 of 2isqA

query
sites
2isqA

I

I

F

A

D

K

D

D

N

V

S

T

L

E

S

L

I

I

G

G

R

N

T

T

P

P

L

L

V

V

K

Y

L

L

N

N

R

N

I

V

A

A

-

E

G

G

N

C

K

V

A

G

T

R

V

V

L

A

A

A

K

K

I

L

E

E

G

M

R

M

N

E

P

P

A

C

Y

S

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

F

A

S

M

M

I

I

W

S

D

D

A

A

E

E

Q

K

R

K

G

G

I

L

L

I

K

K

P

P

G

G

I

E

E

S

I

V

-

L

V

I

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

Y

F

V

T

A

A

R

K

G

G

Y

Y

S

K

L

L

T

I

L

I

T

T

M

M

P

P

D

A

T

S

M

M

S

S

I

T

E

E

R

R

K

I

V

I

L

L

A

L

A

A

F

F

G

G

A

V

Q

E

L

L

F

V

L

L

T

T

P

D

G

P

A

A

E

K

G
|
G

M
|
M

K
|
K

G

G

A

A

I

I

Q

A

R

K

A

A

E

E

I

I

V

L

Q

A

S

K

D

T

P

P

D

N

R

G

Y

Y

F

-

M

M

P

L

Q

Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

K

D

G

T

T

D

G

G

G

S

K

V

I

D

D

V

G

L

F

V

V

S

S

G
|
G

V

I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

Y

Y

I

L

K

K

Q

E

T

-

Q

Q

G

N

K

A

N

N

I

V

L

K

S

L

V

Y

A

G

V

V

E

E

P

P

K

V

E

E

S

S

P

A

V

I

I

L

S

S

Q

G

K

G

L

-

A

-

G

-

Q

-

E

-

L

-

Q

K

P

P

G
|
G

P
|
P

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

D

S

T

V

L

L

D

N

L

V

S

D

V

L

V

I

D

D

R

E

I

V

E

V

Q

Q

V

V

E

S

S

S

M

D

E

E

A

S

V

I

E

D

M

M

A

A

R

R

R

Q

L

L

A

A

Q

L

E

K

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S
|
S

C

S

G

G

A

A

M

A

V

A

A

A

I

I

R

K

L

L

A

A

E

Q

E

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

F

V

V

V

A

I

I

L

F

P
|
P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

V

V

L

L

F

F

D

D

S

A

active site: K44 (= K43), S267 (= S273)
binding pyridoxal-5'-phosphate: K44 (= K43), N75 (= N73), G177 (= G176), G179 (= G178), T180 (= T179), G181 (= G180), T183 (= T182), G223 (= G229), S267 (= S273), P294 (= P300)
binding : T72 (= T70), S73 (= S71), G74 (= G72), T76 (= T74), G122 (= G120), M123 (= M121), K124 (= K122), G217 (= G223), P218 (= P224), H219 (= H225), Q222 (= Q228), G223 (= G229)

Query Sequence

>WP_014148860.1 NCBI__GCF_000968535.2:WP_014148860.1
MNIFDDNSLSIGRTPLVKLNRIAGNKATVLAKIEGRNPAYSVKCRIGAAMIWDAEQRGIL
KPGIEIVEPTSGNTGIALAYVAAARGYSLTLTMPDTMSIERRKVLAAFGAQLFLTPGAEG
MKGAIQRAEEIVQSDPDRYFMPQQFKNPANPAIHEKTTGPEIWNDTDGSVDVLVSGVGTG
GTITGVSRYIKQTQGKNILSVAVEPKESPVISQKLAGQELQPGPHKIQGIGAGFIPDTLD
LSVVDRIEQVESMEAVEMARRLAQEEGILCGISCGAAMVAAIRLAEEDEFAGKTIVVILP
DSGERYLSSVLFDSLH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory