SitesBLAST
Comparing WP_014148949.1 NCBI__GCF_000968535.2:WP_014148949.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q83E11 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
55% identity, 99% coverage: 7:439/439 of query aligns to 3:434/438 of Q83E11
- K21 (= K25) binding phosphoenolpyruvate
- S22 (= S26) binding 3-phosphoshikimate
- R26 (= R30) binding 3-phosphoshikimate
- NSGT 93:96 (= NSGT 96:99) Phosphoenolpyruvate
- G95 (= G98) binding phosphoenolpyruvate
- T96 (= T99) binding phosphoenolpyruvate
- R123 (= R126) binding phosphoenolpyruvate
- S167 (= S172) binding 3-phosphoshikimate
- A168 (= A173) binding 3-phosphoshikimate
- Q169 (= Q174) binding 3-phosphoshikimate; binding phosphoenolpyruvate
- D315 (= D320) binding 3-phosphoshikimate
- K342 (= K347) binding 3-phosphoshikimate
- R346 (= R351) binding phosphoenolpyruvate
- R387 (= R392) binding phosphoenolpyruvate
3slhD 1.70 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with shikimate-3-phosphate and glyphosate
55% identity, 99% coverage: 7:439/439 of query aligns to 5:436/440 of 3slhD
- active site: K23 (= K25), S24 (= S26), D50 (= D52), N95 (= N96), R125 (= R126), D317 (= D320), E345 (= E348), H388 (= H391), R389 (= R392), T415 (= T418)
- binding glyphosate: K23 (= K25), G97 (= G98), T98 (= T99), R125 (= R126), Q171 (= Q174), D317 (= D320), E345 (= E348), R348 (= R351), H388 (= H391), R389 (= R392)
- binding shikimate-3-phosphate: S24 (= S26), R28 (= R30), S169 (= S172), Q171 (= Q174), R196 (= R199), D317 (= D320), K344 (= K347)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S24 (= S26), R28 (= R30), T98 (= T99), Q171 (= Q174), R196 (= R199), D317 (= D320), K344 (= K347)
4egrA 2.50 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with phosphoenolpyruvate
55% identity, 99% coverage: 7:439/439 of query aligns to 5:432/434 of 4egrA
- active site: K23 (= K25), S24 (= S26), D50 (= D52), N95 (= N96), R125 (= R126), D313 (= D320), E341 (= E348), H384 (= H391), R385 (= R392), T411 (= T418)
- binding phosphoenolpyruvate: K23 (= K25), G97 (= G98), T98 (= T99), R125 (= R126), D313 (= D320), E341 (= E348), R344 (= R351), R385 (= R392)
Q9S400 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
50% identity, 94% coverage: 15:425/439 of query aligns to 10:419/427 of Q9S400
- S21 (= S26) binding 3-phosphoshikimate
- R25 (= R30) binding 3-phosphoshikimate
- S166 (= S172) binding 3-phosphoshikimate
- A167 (= A173) binding 3-phosphoshikimate
- Q168 (= Q174) binding 3-phosphoshikimate
- D312 (= D320) binding 3-phosphoshikimate
- K339 (= K347) binding 3-phosphoshikimate
1rf6A Structural studies of streptococcus pneumoniae epsp synthase in s3p- glp bound state (see paper)
50% identity, 94% coverage: 15:425/439 of query aligns to 10:419/427 of 1rf6A
- active site: K20 (= K25), S21 (= S26), D47 (= D52), N90 (= N96), D115 (≠ K121), R120 (= R126), D312 (= D320), E340 (= E348), H384 (= H391), R385 (= R392), T412 (= T418)
- binding glyphosate: K20 (= K25), G92 (= G98), T93 (= T99), R120 (= R126), Q168 (= Q174), D312 (= D320), E340 (= E348), R343 (= R351), H384 (= H391), R385 (= R392)
- binding shikimate-3-phosphate: S21 (= S26), R25 (= R30), S166 (= S172), Q168 (= Q174), R193 (= R199), I311 (= I319), D312 (= D320), K339 (= K347)
1rf4A Structural studies of streptococcus pneumoniae epsp synthase, tetrahedral intermediate bound state (see paper)
50% identity, 94% coverage: 15:425/439 of query aligns to 10:419/427 of 1rf4A
- active site: K20 (= K25), S21 (= S26), D47 (= D52), N90 (= N96), D115 (≠ K121), R120 (= R126), D312 (= D320), E340 (= E348), H384 (= H391), R385 (= R392), T412 (= T418)
- binding (3r,4s,5r)-5-{[(1r)-1-carboxy-2-fluoro-1-(phosphonooxy)ethyl]oxy}-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: K20 (= K25), S21 (= S26), R25 (= R30), G92 (= G98), T93 (= T99), R120 (= R126), S166 (= S172), A167 (= A173), Q168 (= Q174), R193 (= R199), D312 (= D320), K339 (= K347), E340 (= E348), R343 (= R351), H384 (= H391), R385 (= R392)
Q9R4E4 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; CP4 EPSP synthase; EC 2.5.1.19 from Agrobacterium sp. (strain CP4) (see paper)
47% identity, 97% coverage: 8:435/439 of query aligns to 11:448/455 of Q9R4E4
- S29 (= S26) binding 3-phosphoshikimate
- R33 (= R30) binding 3-phosphoshikimate
- A100 (≠ G98) mutation to G: Confers sensitivity to glyphosate allowing glyphosate to bind in its extended, inhibitory conformation.
- S173 (= S172) binding 3-phosphoshikimate
- A174 (= A173) binding 3-phosphoshikimate
- Q175 (= Q174) binding 3-phosphoshikimate
- D326 (= D320) binding 3-phosphoshikimate
- K353 (= K347) binding 3-phosphoshikimate
2pqcA Cp4 epsps liganded with (r)-phosphonate tetrahedral reaction intermediate analog (see paper)
47% identity, 97% coverage: 8:435/439 of query aligns to 6:443/445 of 2pqcA
- active site: K23 (= K25), S24 (= S26), D50 (= D52), N93 (= N96), R123 (= R126), D321 (= D320), E349 (= E348), H399 (= H391), R400 (= R392), T426 (= T418)
- binding [3r-[3a,4a,5b(r*)]]-5-(1-carboxy-1-phosphonoethoxy)-4-hydroxy-3-(phosphonooxy)-1-cyclohexene-1-carboxylic acid: K23 (= K25), S24 (= S26), R28 (= R30), T96 (= T99), R123 (= R126), S168 (= S172), Q170 (= Q174), D321 (= D320), K348 (= K347), E349 (= E348), R352 (= R351), R400 (= R392)
2pqbA Cp4 epsps liganded with (r)-difluoromethyl tetrahedral intermediate analog (see paper)
47% identity, 97% coverage: 8:435/439 of query aligns to 6:443/445 of 2pqbA
- active site: K23 (= K25), S24 (= S26), D50 (= D52), N93 (= N96), R123 (= R126), D321 (= D320), E349 (= E348), H399 (= H391), R400 (= R392), T426 (= T418)
- binding (3r,4s,5r)-5-[(1r)-1-carboxy-2,2-difluoro-1-(phosphonooxy)ethoxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: K23 (= K25), S24 (= S26), R28 (= R30), A95 (≠ G98), T96 (= T99), R123 (= R126), S168 (= S172), Q170 (= Q174), D321 (= D320), K348 (= K347), E349 (= E348), R352 (= R351), R400 (= R392)
2ggaA Cp4 epsp synthase liganded with s3p and glyphosate (see paper)
47% identity, 97% coverage: 8:435/439 of query aligns to 6:443/445 of 2ggaA
- active site: K23 (= K25), S24 (= S26), D50 (= D52), N93 (= N96), R123 (= R126), D321 (= D320), E349 (= E348), H399 (= H391), R400 (= R392), T426 (= T418)
- binding glyphosate: K23 (= K25), A94 (≠ S97), A95 (≠ G98), T96 (= T99), R123 (= R126), D321 (= D320), E349 (= E348), R352 (= R351), R400 (= R392)
- binding shikimate-3-phosphate: S24 (= S26), R28 (= R30), S168 (= S172), A169 (= A173), Q170 (= Q174), R195 (= R199), D321 (= D320), K348 (= K347)
2gg6A Cp4 epsp synthase liganded with s3p (see paper)
47% identity, 97% coverage: 8:435/439 of query aligns to 6:443/445 of 2gg6A