SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_014449906.1 NCBI__GCF_000284315.1:WP_014449906.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
52% identity, 95% coverage: 16:312/312 of query aligns to 12:309/310 of 5xoqA

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5xoqA

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binding : T72 (= T76), S73 (= S77), G74 (= G78), T76 (= T80), M123 (= M127), Q144 (= Q149), R218 (≠ P222), H219 (= H223), Q222 (= Q226), G223 (= G227), A226 (= A230)

Q93244 Cysteine synthase 1; O-acetylserine (thiol)-lyase 1; OAS-TL; EC 2.5.1.47 from Caenorhabditis elegans (see 2 papers)
49% identity, 97% coverage: 11:312/312 of query aligns to 10:313/341 of Q93244

query
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Q93244

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P75 (≠ A75) mutation to L: In n5537; severe loss of protein stability.
A88 (= A88) mutation to V: In n5522; severe loss of protein stability.
S144 (≠ L144) mutation to F: In mr26; susceptible to high levels of hydrogen sulfide.
G181 (= G181) mutation to E: In n5521 and mr23; severe loss of protein stability. Susceptible to high levels of hydrogen sulfide.
G183 (= G183) mutation to R: In n5515; severe loss of protein stability.
G229 (= G229) mutation to E: In mr33; susceptible to high levels of hydrogen sulfide.
R259 (= R259) mutation to K: In n5519; no loss of protein stability. No effect on enzyme activity.
S272 (= S272) mutation to F: In mr29; susceptible to high levels of hydrogen sulfide.
T295 (= T294) mutation to I: In mr39; susceptible to high levels of hydrogen sulfide.

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
47% identity, 96% coverage: 15:312/312 of query aligns to 10:308/310 of P9WP55

query
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P9WP55

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K44 (= K49) modified: N6-(pyridoxal phosphate)lysine
N74 (= N79) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 183:187) binding pyridoxal 5'-phosphate
S266 (≠ T271) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
48% identity, 93% coverage: 15:305/312 of query aligns to 10:301/306 of 2q3dA

query
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2q3dA

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S

active site: K44 (= K49), S266 (≠ T271), P293 (≠ D298)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K49), T71 (= T76), S72 (= S77), N74 (= N79), T75 (= T80), Q144 (= Q149), V177 (= V182), G178 (= G183), T179 (= T184), G180 (= G185), T182 (= T187), G222 (= G227), I223 (= I228), S266 (≠ T271), P293 (≠ D298), D294 (≠ S299)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
48% identity, 93% coverage: 15:304/312 of query aligns to 10:300/300 of 3zeiA

query
sites
3zeiA

L

L

I

I

G

G

N

R

T

T

P

P

L

L

L

V

P

R

L

L

S

R

K

R

V

V

T

T

A

D

G

G

L

A

D

V

R

A

T

D

V

I

W

V

V

A

K

K

L

L

E

E

S

F

R

F

N

N

L

P

G

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

F

A

M

M

I

L

E

Q

Q

A

A

A

E

E

R

Q

D

A

G

G

R

L

L

I

G

K

K

P

D

D

G

T

R

I

I

I

V

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

Q

M

I

V

A

C

V

A

L

A

K

R

G

G

Y

Y

A

R

I

C

T

V

I

L

V

T

M

M

P

P

E

E

G

T

V

M

S

S

G

L

E

E

R

R

V

R

S

M

H

L

L

L

K

R

A

A

L

Y

G

G

A

A

E

E

V

L

L

I

L

L

T

T

P

P

S

G

R

A

E

D

G

G

M

M

V

S

G

G

A

A

I
|
I

G

A

K

K

A

A

T

E

E

E

M

L

E

A

K

K

S

T

E

D

K

Q

G

R

L

Y

F

F

M

V

P

P

R

Q

Q
|
Q

F
|
F

E

E

N

N

P

P

S

A

N

N

P

P

E

A

S

I

H

H

Y

R

R

V

T

T

G

A

P

E

E

E

I

V

F

W

R

R

Q

D

L

T

G

D

R

G

V

K

P

V

D

D

G

I

F

V

V

V

A

A

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

V

V

G

A

R

Q

Y

V

L

I

R

K

E

E

K

R

K

K

R

P

D

S

L

A

K

R

I

F

W

V

A

A

L

V

E

E

P

P

A

A

S

S

P

P

V

V

L

L

S

S

G

G

S

Q

P
x
K

G

G

P

P

H

H

R

P

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F
|
F

E

V

P

P

R

P

T

V

F

L

N

D

R

Q

S

D

V

L

V

V

D

D

R

E

I

I

E

I

K

T

I

V

S

G

D

N

R

E

E

D

A

A

I

L

D

N

M

V

A

A

R

R

L

L

S

A

Q

R

E

E

G

G

I

L

M

L

A

V

G

G

I

I

T
x
S

S

S

G

G

A

A

N

A

V

T

V

V

G

A

A

A

L

L

R

Q

L

V

A

A

G

R

E

R

L

P

P

E

P

N

G

A

S

G

H

K

V

L

V

I

T

V

I

V

V

V

C

L

D
x
P

S
x
D

F

F

-

G

E

E

R

R

Y

Y

F

L

active site: K44 (= K49), S266 (≠ T271), P293 (≠ D298)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T76), S72 (= S77), I126 (= I131), Q144 (= Q149), F145 (= F150), K215 (≠ P220), G222 (= G227), A225 (= A230), F227 (= F232)
binding pyridoxal-5'-phosphate: K44 (= K49), N74 (= N79), V177 (= V182), G178 (= G183), T179 (= T184), G180 (= G185), T182 (= T187), G222 (= G227), S266 (≠ T271), P293 (≠ D298), D294 (≠ S299)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
48% identity, 93% coverage: 15:304/312 of query aligns to 10:300/300 of 2q3cA

query
sites
2q3cA

L

L

I

I

G

G

N

R

T

T

P

P

L

L

L

V

P

R

L

L

S

R

K

R

V

V

T

T

A

D

G

G

L

A

D

V

R

A

T

D

V

I

W

V

V

A

K

K

L

L

E

E

S

F

R

F

N

N

L

P

G

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

F

A

M

M

I

L

E

Q

Q

A

A

A

E

E

R

Q

D

A

G

G

R

L

L

I

G

K

K

P

D

D

G

T

R

I

I

I

V

L

E

E

A

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

Q

M

I

V

A

C

V

A

L

A

K

R

G

G

Y

Y

A

R

I

C

T

V

I

L

V

T

M

M

P

P

E

E

G

T

V

M

S

S

G

L

E

E

R

R

V

R

S

M

H

L

L

L

K

R

A

A

L

Y

G

G

A

A

E

E

V

L

L

I

L

L

T

T

P

P

S

G

R

A

E

D

G

G

M
|
M

V

S

G

G

A

A

I

I

G

A

K

K

A

A

T

E

E

E

M

L

E

A

K

K

S

T

E

D

K

Q

G

R

L

Y

F

F

M

V

P

P

R

Q

Q
|
Q

F

F

E

E

N

N

P

P

S

A

N

N

P

P

E

A

S

I

H

H

Y

R

R

V

T

T

G

A

P

E

E

E

I

V

F

W

R

R

Q

D

L

T

G

D

R

G

V

K

P

V

D

D

G

I

F

V

V

V

A

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

S

T

G

G

V

V

G

A

R

Q

Y

V

L

I

R

K

E

E

K

R

K

K

R

P

D

S

L

A

K

R

I

F

W

V

A

A

L

V

E

E

P

P

A

A

S

S

P

P

V

V

L

L

S

S

G

G

S

Q

P
x
K

G

G

P

P

H

H

R

P

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

E

V

P

P

R

P

T

V

F

L

N

D

R

Q

S

D

V

L

V

V

D

D

R

E

I

I

E

I

K

T

I

V

S

G

D

N

R

E

E

D

A

A

I

L

D

N

M

V

A

A

R

R

L

L

S

A

Q

R

E

E

G

G

I

L

M

L

A

V

G

G

I

I

T
x
S

S

S

G

G

A

A

N

A

V

T

V

V

G

A

A

A

L

L

R

Q

L

V

A

A

G

R

E

R

L

P

P

E

P

N

G

A

S

G

H

K

V

L

V

I

T

V

I

V

V

V

C

L

D
x
P

S

D

F

F

-

G

E

E

R

R

Y

Y

F

L

active site: K44 (= K49), S266 (≠ T271), P293 (≠ D298)
binding : T71 (= T76), S72 (= S77), G73 (= G78), T75 (= T80), M122 (= M127), Q144 (= Q149), K215 (≠ P220), G222 (= G227), A225 (= A230)

6ahiB Crystal structure of o-acetylserine dependent cystathionine beta- synthase from helicobacter pylori. (see paper)
42% identity, 95% coverage: 16:310/312 of query aligns to 11:306/306 of 6ahiB

query
sites
6ahiB

I

I

G

G

N

R

T

T

P

P

L

V

L

F

P

K

L

F

S

T

K

N

-

K

-

D

V

Y

T

P

A

I

G

P

L

L

D

N

R

S

T

A

V

I

W

Y

V

A

K

K

L

L

E

E

S

H

R

L

N

N

L

P

G

G

S

S

V

V

K
|
K

D

D

R

R

P

L

A

G

L

Q

F

Y

M

L

I

I

E

G

Q

E

A

G

E

F

R

K

D

T

G

G

R

K

L

I

G

T

K

S

D

K

G

T

R

T

I

I

V

I

E

E

A

P

T
|
T

S
x
A

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

Q

L

I

V

A

A

V

I

L

K

K

H

G

H

Y

L

A

K

I

T

T

I

I

F

V

V

M

V

P

P

E

E

G

K

V

F

S

S

G

T

E

E

R
x
K

V

Q

S

Q

H

I

L

M

K

R

A

A

L

L

G

G

A

A

E

L

V

V

L

I

L

N

T

T

P

P

S

T

R

S

E

E

G

G

M

I

V

S

G

G

A

A

I

I

G

K

K

K

A

S

T

K

E

E

M

L

E

A

K

E

S

S

E

I

K

P

G

D

L

S

F

Y

M

L

P

P

R

L

Q

Q

F

F

E

E

N

N

P

P

S

D

N

N

P

P

E

A

S

A

H

Y

Y

Y

R

H

T

T

T

L

G

A

P

P

E

E

I

I

F

V

R

Q

Q

E

L

L

G

G

R

T

V

N

P

L

D

T

G

S

F

F

V

V

A

A

G

G

V

I

G

G

T

S

G

G

T

T

I

F

S

A

G

G

V

T

G

A

R

R

Y

Y

L

L

R

K

E

E

K

R

K

I

R

P

D

A

L

I

K

R

I

L

W

I

A

G

L

V

E

E

P

P

A

E

A

G

S

S

P

-

V

I

L

L

S

N

G

G

S

E

P

P

G

G

P

P

H

H

R

E

I

I

Q

E

G

G

I

I

G

G

A

V

G

E

F

F

E

I

P

P

R

P

T

F

F

F

N

E

R

N

S

L

V

D

V

I

D

D

R

G

I

F

E

E

K

T

I

I

S

S

D

D

R

E

E

E

A

G

I

F

D

S

M

Y

A

T

R

R

R

K

L

L

S

A

Q

K

E

K

E

N

G

G

I

L

M

L

A

V

G

G

I

S

T
x
S

S

S

G

G

A

A

N

A

V

F

V

V

G

A

A

A

L

L

R

K

L

E

A

A

G

Q

E

R

L

L

P

P

P

E

G

G

S

S

H

Q

V

V

V

L

T

T

I

I

V

F

C

P

D

D

S

V

F

A

E

D

R

R

Y

Y

F

L

S

S

M

K

E

G

K

I

Y

Y

L

L

active site: K46 (= K49), S267 (≠ T271)
binding methionine: K46 (= K49), T73 (= T76), A74 (≠ S77), G75 (= G78), N76 (= N79), T77 (= T80), K104 (≠ R107)

5xemA Crystal structure of a hydrogen sulfide-producing enzyme (fn1220) from fusobacterium nucleatum in complex with l-lanthionine-plp schiff base
45% identity, 94% coverage: 13:305/312 of query aligns to 7:301/302 of 5xemA

query
sites
5xemA

L

I

A

D

L

L

I

I

G

G

N

N

T

T

P

P

L

L

L

V

P

K

L

I

S

N

K

N

V

I

T

D

A

T

G

-

L

F

D

G

R

N

T

E

V

I

W

Y

V

V

K

K

L

L

E

E

S

G

R

S

N

N

L

P

G

G

G

R

S

S

V

T

K
|
K

D

D

R

R

P

I

A

A

L

L

F

K

M

M

I

I

E

E

Q

E

A

A

E

E

R

K

D

E

G

G

R

L

L

I

G

D

K

K

D

D

G

T

R

V

I

I

V

I

E

E

A

A

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

Q

M

I

I

A

C

V

A

L

V

K

K

G

N

Y

Y

A

K

I

L

T

K

I

I

V

V

M

M

P

P

E

D

G

T

V

M

S

S

G

I

E

E

R

R

V

I

S

Q

H

L

L

M

K

R

A

A

L

Y

G

G

A

T

E

E

V

V

L

I

L

L

T

T

P

D

S

G

R

S

E

L

G

G

M
|
M

V

K

G

A

A

C

I

L

G

E

K

K

A

L

T

E

E

E

M

L

E

K

K

K

S

N

E

E

K

K

G

K

L

Y

F

F

M

V

P

P

R

N

Q
|
Q

F

F

E

T

N

N

P

V

S

N

N

N

P

P

E

K

S

A

H

H

Y

Y

R

E

T

T

G

A

P

E

E

E

I

I

F

L

R

K

Q

D

L

L

G

N

R

N

V

K

P

V

D

D

G

V

F

F

V

I

A

C

G

G

V
x
T

G
|
G

T
|
T

G
|
G

G

G

T
x
S

I

F

S

S

G

G

V

T

G

A

R

K

Y

K

L

L

R

K

E

E

K

K

K

L

R

P

D

N

L

I

K

K

I

T

W

F

A

P

L

V

E

E

P

P

A

A

A

S

S

S

P

P

V

L

L

L

S

S

G

K

G

G

S

Y

P

I

G

G

P

P

H

H

R

K

I

I

Q

Q

G
|
G

I
x
M

G
|
G

A

M

-
x
S

-

I

G

G

F

G

E

I

P

P

R

A

T

V

F

Y

N

D

R

G

S

S

V

L

V

A

D

D

R

D

I

I

E

L

K

V

I

C

S

E

D

D

R

D

E

D

A

A

I

F

D

E

M

M

A

M

R

R

R

E

L

L

S

S

Q

F

E

K

E

E

G

G

I

I

M

L

A

G

G

G

I

I

T
x
S

S

T

G

G

A

A

N

T

V

F

V

K

G

A

A

A

L

L

R

D

L

Y

A

S

G

K

E

E

-

N

L

A

P

D

P

K

G

G

S

L

H

K

V

I

V

V

T

V

I

L

V

S

C
x
T

D
|
D

S

S

F

G

E

E

R

K

Y

Y

F
x
L

S
|
S

binding (2R)-2-azanyl-3-[(2R)-2-azanyl-3-oxidanyl-3-oxidanylidene-propyl]sulfanyl-propanoic acid: K42 (= K49), T69 (= T76), S70 (= S77), N72 (= N79), T73 (= T80), M120 (= M127), Q142 (= Q149), G176 (= G183), T177 (= T184), G220 (= G227), M221 (≠ I228), G222 (= G229), S224 (vs. gap)
binding calcium ion: L300 (≠ F304), S301 (= S305)
binding pyridoxal-5'-phosphate: K42 (= K49), N72 (= N79), T175 (≠ V182), G176 (= G183), T177 (= T184), G178 (= G185), S180 (≠ T187), G220 (= G227), S266 (≠ T271), T293 (≠ C297), D294 (= D298)

Sites not aligning to the query:

binding calcium ion: 302

5xenA Crystal structure of a hydrogen sulfide-producing enzyme (fn1220) from fusobacterium nucleatum in complex with l-serine-plp schiff base
44% identity, 94% coverage: 13:304/312 of query aligns to 7:300/300 of 5xenA

query
sites
5xenA

L

I

A

D

L

L

I

I

G

G

N

N

T

T

P

P

L

L

L

V

P

K

L

I

S

N

K

N

V

I

T

D

A

T

G

-

L

F

D

G

R

N

T

E

V

I

W

Y

V

V

K

K

L

L

E

E

S

G

R

S

N

N

L

P

G

G

G

R

S

S

V

T

K
|
K

D

D

R

R

P

I

A

A

L

L

F

K

M

M

I

I

E

E

Q

E

A

A

E

E

R

K

D

E

G

G

R

L

L

I

G

D

K

K

D

D

G

T

R

V

I

I

V

I

E

E

A

A

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

Q

M

I

I

A

C

V

A

L

V

K

K

G

N

Y

Y

A

K

I

L

T

K

I

I

V

V

M

M

P

P

E

D

G

T

V

M

S

S

G

I

E

E

R

R

V

I

S

Q

H

L

L

M

K

R

A

A

L

Y

G

G

A

T

E

E

V

V

L

I

L

L

T

T

P

D

S

G

R

S

E

L

G

G

M

M

V

K

G

A

A

C

I

L

G

E

K

K

A

L

T

E

E

E

M

L

E

K

K

K

S

N

E

E

K

K

G

K

L

Y

F

F

M

V

P

P

R

N

Q
|
Q

F

F

E

T

N

N

P

V

S

N

N

N

P

P

E

K

S

A

H

H

Y

Y

R

E

T

T

G

A

P

E

E

E

I

I

F

L

R

K

Q

D

L

L

G

N

R

N

V

K

P

V

D

D

G

V

F

F

V

I

A

C

G

G

V
x
T

G
|
G

T
|
T

G
|
G

G

G

T
x
S

I

F

S

S

G

G

V

T

G

A

R

K

Y

K

L

L

R

K

E

E

K

K

K

L

R

P

D

N

L

I

K

K

I

T

W

F

A

P

L

V

E

E

P

P

A

A

A

S

S

S

P

P

V

L

L

L

S

S

G

K

G

G

S

Y

P

I

G

G

P

P

H

H

R

K

I

I

Q

Q

G
|
G

I

M

G

G

A

M

-

S

-

I

G

G

F

G

E

I

P

P

R

A

T

V

F

Y

N

D

R

G

S

S

V

L

V

A

D

D

R

D

I

I

E

L

K

V

I

C

S

E

D

D

R

D

E

D

A

A

I

F

D

E

M

M

A

M

R

R

R

E

L

L

S

S

Q

F

E

K

E

E

G

G

I

I

M

L

A

G

G

G

I

I

T
x
S

S

T

G

G

A

A

N

T

V

F

V

K

G

A

A

A

L

L

R

D

L

Y

A

S

G

K

E

E

-

N

L

A

P

D

P

K

G

G

S

L

H

K

V

I

V

V

T

V

I

L

V

S

C
x
T

D
|
D

S

S

F

G

E

E

R

K

Y

Y

F

L

binding pyridoxal-5'-phosphate: K42 (= K49), K42 (= K49), N72 (= N79), N72 (= N79), T175 (≠ V182), T175 (≠ V182), G176 (= G183), G176 (= G183), T177 (= T184), T177 (= T184), G178 (= G185), G178 (= G185), S180 (≠ T187), S180 (≠ T187), G220 (= G227), G220 (= G227), S266 (≠ T271), S266 (≠ T271), T293 (≠ C297), T293 (≠ C297), D294 (= D298), D294 (= D298)
binding serine: K42 (= K49), T69 (= T76), S70 (= S77), N72 (= N79), T73 (= T80), Q142 (= Q149)

8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
46% identity, 99% coverage: 5:312/312 of query aligns to 6:314/329 of 8b9wA

query
sites
8b9wA

E

Q

G

N

D

D

V

V

I

A

R

P

N

S

P

V

L

D

A

A

L

L

I

I

G

G

N

N

T

T

P

P

L

A

L

V

P

Y

L

L

S

R

K

N

V

M

T

N

A

K

G

T

L

A

D

-

R

A

T

T

V

I

W

V

V

L

K

K

L

L

E

E

S

S

R

E

N

N

L

P

G

M

G

A

S

S

V

V

K
|
K

D

D

R

R

P

L

A

A

L

Y

F

A

M

I

I

Y

E

D

Q

K

A

A

E

E

R

K

D

E

G

G

R

K

L

I

G

-

K

K

D

R

G

G

R

K

-

S

-

V

I

I

V

V

E

E

A

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

Q

L

I

L

A

G

V

A

L

V

K

R

G

G

Y

Y

A

K

I

V

T

I

I

I

V

T

M

M

P

P

E

D

G

S

V

M

S

S

G

M

E

E

R

R

V

R

S

S

H

L

L

L

K

R

A

I

L

F

G

G

A

A

E

E

V

L

L

I

L

L

T

T

P

P

S

A

R

S

E

L

G

G

M

M

V

K

G

G

A

A

I

V

G

A

K

M

A

A

T

N

E

R

M

I

E

V

K

S

S

N

E

N

K

P

G

D

L

A

F

V

M

L

P

A

R

D

Q

Q

F

F

E

C

N

T

P

K

S

Y

N

N

P

A

E

Q

S

I

H

H

Y

E

R

E

T

T

G

G

P

P

E

E

I

I

F

W

R

R

Q

Q

L

T

G

K

R

G

V

H

P

V

D

D

G

C

F

F

V

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

S

T

G

G

V

V

G

A

R

R

Y

F

L

L

R

K

E

S

K

V

K

G

R

C

D

N

L

A

K

N

I

I

W

V

A

A

L

V

E

E

P

P

A

Q

A

E

S

S

P

P

V

V

L

L

S

S

G

G

S

R

P

P

G

G

P

P

H

H

R

R

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

E

V

P

P

R

E

T

V

F

L

N

D

R

R

S

E

V

L

V

V

D

D

R

E

I

I

E

I

K

Q

I

V

S

S

D

S

R

D

E

K

A

A

I

I

D

E

M

T

A

A

R

Q

R

K

L

L

S

S

Q

R

E

M

E

D

G

G

I

I

M

F

A

C

G

G

I

F

T
x
S

S

A

G

G

A

A

N

N

V

V

V

F

G

A

A

A

L

L

R

K

L

I

A

A

G

-

E

E

L

R

P

P

-

E

-

M

P

A

G

G

S

K

H

T

V

I

V

V

T

T

I

V

V

I

C
x
P

D

S

S

F

F

G

E

E

R

R

Y

Y

F

L

S

S

M

T

E

A

K

L

Y

Y

L

A

N

S

L

V

binding pyridoxal-5'-phosphate: K49 (= K49), N80 (= N79), G184 (= G183), T185 (= T184), G186 (= G185), T188 (= T187), G228 (= G227), S272 (≠ T271), P299 (≠ C297)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
46% identity, 96% coverage: 8:305/312 of query aligns to 5:304/322 of P47998

query
sites
P47998

V

I

I

A

R

K

N

D

P

V

L

T

A

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

L

V

P

Y

L

L

S

N

K

N

V

V

T

A

A

E

G

G

L

C

D

V

R

G

T

R

V

V

W

A

V

A

K

K

L

L

E

E

S

M

R

M

N

E

L

P

G

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

F

F

S

M

M

I

I

E

S

Q

D

A

A

E

E

R

K

D

K

G

G

R

L

L

I

G

K

K

P

-

G

D

E

G

S

R

V

I

L

V

I

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

Q

F

I

T

A

A

V

A

L

A

K

K

G

G

Y

Y

A

K

I

L

T

I

I

I

V

T

M

M

P

P

E

A

G

S

V

M

S

S

G

T

E

E

R

R

V

R

S

I

H

I

L

L

K

L

A

A

L

F

G

G

A

V

E

E

V

L

L

V

L

L

T

T

P

D

S

P

R

A

E

K

G

G

M

M

V

K

G

G

A

A

I

I

G

A

K

K

A

A

T

E

E

E

M

I

E

L

K

A

S

K

E

T

K

P

G

N

L

G

F

Y

M

M

P

L

R

Q

Q
|
Q

F

F

E

E

N

N

P

P

S

A

N

N

P

P

E

K

S

I

H
|
H

Y

Y

R

E

T

T

G
|
G

P

P

E

E

I

I

F

W

R

K

Q

G

L

T

G

G

R

G

V

K

P

I

D

D

G

G

F

F

V

V

A

S

G

G

V

I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

S

T

G

G

V

A

G

G

R

K

Y

Y

L

L

R

K

E

E

K

Q

K

N

R

A

D

N

L

V

K

K

I

L

W

Y

A

G

L

V

E

E

P

P

A

V

A

E

S

S

P

A

V

I

L

L

S

S

G

G

S
x
K

P

P

G

G

P

P

H
|
H

R
x
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

E

I

P

P

R

S

T

V

F

L

N

N

R

V

S

D

V

L

V

I

D

D

R

E

I

V

E

V

K

Q

I

V

S

S

D

S

R

D

E

E

A

S

I

I

D

D

M

M

A

A

R

R

R

Q

L

L

S

A

Q

L

E

K

E

E

G

G

I

L

M

L

A

V

G

G

I

I

T
x
S

S

S

G

G

A

A

N

A

V

A

G

A

A

A

L

I

R

K

L

L

A

A

G

Q

E

R

L

P

P

E

P

N

G

A

S

G

H

K

V

L

V

F

T

V

I

A

V

I

C

F

D

P

S

S

F

F

-

G

E

E

R

R

Y

Y

F

L

S

S

K46 (= K49) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T76) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S77) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N79) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T80) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q149) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H159) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G164) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 183:187) binding pyridoxal 5'-phosphate
T182 (= T184) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T187) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ S219) mutation to A: Impaired interaction with SAT1.
H221 (= H223) mutation to A: Impaired interaction with SAT1.
K222 (≠ R224) mutation to A: Impaired interaction with SAT1.
S269 (≠ T271) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
46% identity, 96% coverage: 8:305/312 of query aligns to 3:302/320 of 2isqA

query
sites
2isqA

V

I

I

A

R

K

N

D

P

V

L

T

A

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

L

V

P

Y

L

L

S

N

K

N

V

V

T

A

A

E

G

G

L

C

D

V

R

G

T

R

V

V

W

A

V

A

K

K

L

L

E

E

S

M

R

M

N

E

L

P

G

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

F

F

S

M

M

I

I

E

S

Q

D

A

A

E

E

R

K

D

K

G

G

R

L

L

I

G

K

K

P

-

G

D

E

G

S

R

V

I

L

V

I

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

Q

F

I

T

A

A

V

A

L

A

K

K

G

G

Y

Y

A

K

I

L

T

I

I

I

V

T

M

M

P

P

E

A

G

S

V

M

S

S

G

T

E

E

R

R

V

R

S

I

H

I

L

L

K

L

A

A

L

F

G

G

A

V

E

E

V

L

L

V

L

L

T

T

P

D

S

P

R

A

E

K

G
|
G

M
|
M

V
x
K

G

G

A

A

I

I

G

A

K

K

A

A

T

E

E

E

M

I

E

L

K

A

S

K

E

T

K

P

G

N

L

G

F

Y

M

M

P

L

R

Q

Q

Q

F

F

E

E

N

N

P

P

S

A

N

N

P

P

E

K

S

I

H

H

Y

Y

R

E

T

T

G

G

P

P

E

E

I

I

F

W

R

K

Q

G

L

T

G

G

R

G

V

K

P

I

D

D

G

G

F

F

V

V

A

S

G
|
G

V

I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

V

A

G

G

R

K

Y

Y

L

L

R

K

E

E

K

Q

K

N

R

A

D

N

L

V

K

K

I

L

W

Y

A

G

L

V

E

E

P

P

A

V

A

E

S

S

P

A

V

I

L

L

S

S

G

G

S

K

P

P

G
|
G

P
|
P

H
|
H

R

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A

A

G

G

F

F

E

I

P

P

R

S

T

V

F

L

N

N

R

V

S

D

V

L

V

I

D

D

R

E

I

V

E

V

K

Q

I

V

S

S

D

S

R

D

E

E

A

S

I

I

D

D

M

M

A

A

R

R

R

Q

L

L

S

A

Q

L

E

K

E

E

G

G

I

L

M

L

A

V

G

G

I

I

T
x
S

S

S

G

G

A

A

N

A

V

A

G

A

A

A

L

I

R

K

L

L

A

A

G

Q

E

R

L

P

P

E

P

N

G

A

S

G

H

K

V

L

V

F

T

V

I

A

V

I

C

F

D
x
P

S

S

F

F

-

G

E

E

R

R

Y

Y

F

L

S

S

active site: K44 (= K49), S267 (≠ T271)
binding pyridoxal-5'-phosphate: K44 (= K49), N75 (= N79), G177 (= G181), G179 (= G183), T180 (= T184), G181 (= G185), T183 (= T187), G223 (= G227), S267 (≠ T271), P294 (≠ D298)
binding : T72 (= T76), S73 (= S77), G74 (= G78), T76 (= T80), G122 (= G126), M123 (= M127), K124 (≠ V128), G217 (= G221), P218 (= P222), H219 (= H223), Q222 (= Q226), G223 (= G227)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
49% identity, 94% coverage: 16:309/312 of query aligns to 7:301/302 of 2efyA

query
sites
2efyA

I

I

G

G

N

K

T

T

P

P

L

V

P

R

L

L

S

A

K

K

V

V

T

V

A

E

G

P

L

D

D

M

R

A

T

E

V

V

W

W

V

V

K

K

L

L

E

E

S

G

R

L

N

N

L

P

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

F

Y

M

M

I

I

E

K

Q

D

A

A

E

E

R

E

D

R

G

G

-

I

-

L

R

R

L

P

G

G

K

S

D

G

G

Q

R

V

I

I

V

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

Q

M

I

I

A

A

V

A

L

S

K

R

G

G

Y

Y

A

R

I

L

T

I

I

L

V

T

M

M

P

P

E

A

G

Q

V

M

S

S

G

E

E

E

R

R

V

K

S

R

H

V

L

L

K

K

A

A

L

F

G

G

A

A

E

E

V

L

L

V

L

L

T

T

P

D

S

P

R

E

E

R

G

R

M

M

V

L

G

A

A

A

I

R

G

E

K

E

A

A

T

L

E

R

M

L

E

-

K

K

S

E

E

E

K

L

G

G

L

A

F

F

M

M

P

P

R

D

Q
|
Q

F

F

E

K

N

N

P

P

S

A

N

N

P

V

E

R

S

A

H

H

Y

Y

R

E

T

T

G

G

P

P

E

E

I

L

F

Y

R

E

Q

A

L

L

-

E

G

G

R

R

V

I

P

-

D

D

G

A

F

F

V

V

A
x
Y

G

G

V

S

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

V

V

G

G

R

R

Y

Y

L

L

R

K

E

E

K

R

K

I

R

P

D

H

L

V

K

K

I

V

W

I

A

A

L

V

E
|
E

P

P

A

A

A

R

S
|
S

P

N

V

V

L

L

S

S

G

G

S

K

P

M

G

G

P

Q

H

H

R

G

I

F

Q

Q

G
|
G

I
x
M

G

G

A
x
P

G

G

F

F

E

I

P

P

R

E

T

N

F

L

N

D

R

L

S

S

V

L

D

D

R

G

I

V

E

I

K

Q

I

V

S

W

D

E

R

E

E

D

A

A

I

F

D

P

M

L

A

A

R

R

L

L

S

A

Q

R

E

E

G

G

I

L

M

F

A

L

G

G

I

M

T
x
S

S

S

G

G

A

G

N

I

V

V

V

W

G

A

A

A

L

L

R

Q

L

V

A

A

G

R

E

E

L

L

P

G

P

P

G

G

S

K

H

R

V

V

V

A

T

C

I

I

V

S

C
x
P

D
|
D

S

G

F

G

E

W

R

K

Y

Y

F

L

S

S

M

T

E

P

K

L

Y

Y

active site: K40 (= K49), S70 (= S77), E200 (= E208), S204 (= S212), S263 (≠ T271)
binding 5-oxohexanoic acid: T69 (= T76), G71 (= G78), T73 (= T80), Q141 (= Q149), G175 (= G183), G219 (= G227), M220 (≠ I228), P222 (≠ A230)
binding pyridoxal-5'-phosphate: K40 (= K49), N72 (= N79), Y172 (≠ A180), G175 (= G183), T176 (= T184), G177 (= G185), T179 (= T187), G219 (= G227), S263 (≠ T271), P289 (≠ C297), D290 (= D298)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
49% identity, 94% coverage: 16:309/312 of query aligns to 7:301/302 of 2ecqA

query
sites
2ecqA

I

I

G

G

N

K

T

T

P

P

L

V

P

R

L

L

S

A

K

K

V

V

T

V

A

E

G

P

L

D

D

M

R

A

T

E

V

V

W

W

V

V

K

K

L

L

E

E

S

G

R

L

N

N

L

P

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

F

Y

M

M

I

I

E

K

Q

D

A

A

E

E

R

E

D

R

G

G

-

I

-

L

R

R

L

P

G

G

K

S

D

G

G

Q

R

V

I

I

V

V

E

E

A

P

T

T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

Q

M

I

I

A

A

V

A

L

S

K

R

G

G

Y

Y

A

R

I

L

T

I

I

L

V

T

M

M

P

P

E

A

G

Q

V

M

S

S

G

E

E

E

R

R

V

K

S

R

H

V

L

L

K

K

A

A

L

F

G

G

A

A

E

E

V

L

L

V

L

L

T

T

P

D

S

P

R

E

E

R

G

R

M

M

V

L

G

A

A

A

I

R

G

E

K

E

A

A

T

L

E

R

M

L

E

-

K

K

S

E

E

E

K

L

G

G

L

A

F

F

M

M

P

P

R

D

Q
|
Q

F

F

E

K

N

N

P

P

S

A

N

N

P

V

E

R

S

A

H

H

Y

Y

R

E

T

T

G

G

P

P

E

E

I

L

F

Y

R

E

Q

A

L

L

-

E

G

G

R

R

V

I

P

-

D

D

G

A

F

F

V

V

A
x
Y

G
|
G

V

S

G
|
G

T
|
T

G

G

T
|
T

I

I

S

T

G

G

V

V

G

G

R

R

Y

Y

L

L

R

K

E

E

K

R

K

I

R

P

D

H

L

V

K

K

I

V

W

I

A

A

L

V

E
|
E

P

P

A

A

A

R

S
|
S

P

N

V

V

L

L

S

S

G

G

S

K

P

M

G

G

P

Q

H

H

R

G

I

F

Q

Q

G
|
G

I

M

G

G

A

P

G

G

F

F

E

I

P

P

R

E

T

N

F

L

N

D

R

L

S

S

V

L

D

D

R

G

I

V

E

I

K

Q

I

V

S

W

D

E

R

E

E

D

A

A

I

F

D

P

M

L

A

A

R

R

L

L

S

A

Q

R

E

E

G

G

I

L

M

F

A

L

G

G

I

M

T
x
S

S

S

G

G

A

G

N

I

V

V

V

W

G

A

A

A

L

L

R

Q

L

V

A

A

G

R

E

E

L

L

P

G

P

P

G

G

S

K

H

R

V

V

V

A

T

C

I

I

V

S

C
x
P

D

D

S

G

F

G

E

W

R

K

Y

Y

F

L

S

S

M

T

E

P

K

L

Y

Y

active site: K40 (= K49), S70 (= S77), E200 (= E208), S204 (= S212), S263 (≠ T271)
binding (3s)-3-hydroxybutanoic acid: K40 (= K49), G71 (= G78), T73 (= T80), Q141 (= Q149), G219 (= G227)
binding pyridoxal-5'-phosphate: K40 (= K49), N72 (= N79), Y172 (≠ A180), G173 (= G181), G175 (= G183), T176 (= T184), T179 (= T187), G219 (= G227), S263 (≠ T271), P289 (≠ C297)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
49% identity, 94% coverage: 16:309/312 of query aligns to 7:301/302 of 2ecoA

query
sites
2ecoA

I

I

G

G

N

K

T

T

P

P

L

V

P

R

L

L

S

A

K

K

V

V

T

V

A

E

G

P

L

D

D

M

R

A

T

E

V

V

W

W

V

V

K

K

L

L

E

E

S

G

R

L

N

N

L

P

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

F

Y

M

M

I

I

E

K

Q

D

A

A

E

E

R

E

D

R

G

G

-

I

-

L

R

R

L

P

G

G

K

S

D

G

G

Q

R

V

I

I

V

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

Q

M

I

I

A

A

V

A

L

S

K

R

G

G

Y

Y

A

R

I

L

T

I

I

L

V

T

M

M

P

P

E

A

G

Q

V

M

S

S

G

E

E

E

R

R

V

K

S

R

H

V

L

L

K

K

A

A

L

F

G

G

A

A

E

E

V

L

L

V

L

L

T

T

P

D

S

P

R

E

E

R

G

R

M

M

V

L

G

A

A

A

I

R

G

E

K

E

A

A

T

L

E

R

M

L

E

-

K

K

S

E

E

E

K

L

G

G

L

A

F

F

M

M

P

P

R

D

Q
|
Q

F

F

E

K

N

N

P

P

S

A

N

N

P

V

E

R

S

A

H

H

Y

Y

R

E

T

T

G

G

P

P

E

E

I

L

F

Y

R

E

Q

A

L

L

-

E

G

G

R

R

V

I

P

-

D

D

G

A

F

F

V

V

A
x
Y

G

G

V

S

G
|
G

T
|
T

G

G

T
|
T

I

I

S

T

G

G

V

V

G

G

R

R

Y

Y

L

L

R

K

E

E

K

R

K

I

R

P

D

H

L

V

K

K

I

V

W

I

A

A

L

V

E
|
E

P

P

A

A

A

R

S
|
S

P

N

V

V

L

L

S

S

G

G

S

K

P

M

G

G

P

Q

H

H

R

G

I

F

Q

Q

G
|
G

I

M

G

G

A

P

G

G

F

F

E

I

P

P

R

E

T

N

F

L

N

D

R

L

S

S

V

L

D

D

R

G

I

V

E

I

K

Q

I

V

S

W

D

E

R

E

E

D

A

A

I

F

D

P

M

L

A

A

R

R

L

L

S

A

Q

R

E

E

G

G

I

L

M

F

A

L

G

G

I

M

T
x
S

S

S

G

G

A

G

N

I

V

V

V

W

G

A

A

A

L

L

R

Q

L

V

A

A

G

R

E

E

L

L

P

G

P

P

G

G

S

K

H

R

V

V

V

A

T

C

I

I

V

S

C
x
P

D
|
D

S

G

F

G

E

W

R

K

Y

Y

F

L

S

S

M

T

E

P

K

L

Y

Y

active site: K40 (= K49), S70 (= S77), E200 (= E208), S204 (= S212), S263 (≠ T271)
binding 4-methyl valeric acid: K40 (= K49), T69 (= T76), G71 (= G78), T73 (= T80), Q141 (= Q149), G175 (= G183), T176 (= T184), G219 (= G227)
binding pyridoxal-5'-phosphate: K40 (= K49), N72 (= N79), Y172 (≠ A180), G175 (= G183), T176 (= T184), T179 (= T187), G219 (= G227), S263 (≠ T271), P289 (≠ C297), D290 (= D298)

Q9FS29 Bifunctional L-3-cyanoalanine synthase/cysteine synthase 2, mitochondrial; EC 2.5.1.47; EC 4.4.1.9 from Solanum tuberosum (Potato) (see paper)
43% identity, 99% coverage: 4:312/312 of query aligns to 24:334/347 of Q9FS29

query
sites
Q9FS29

P

P

E

H

G

T

D

N

V

I

I

K

R

S

N

E

P

V

L

S

A

Q

L

L

I

I

G

G

N

K

T

T

P

P

L

M

L

V

P

Y

L

L

S

K

K

K

V

V

T

T

A

E

G

G

L

C

D

G

R

A

T

Y

V

I

W

A

V

V

K

K

L

Q

E

E

S

M

R

F

N

Q

L

P

G

T

G

S

S

S

V

I

K

K

D

D

R

R

P

P

A

A

L

L

F

A

M

M

I

I

E

N

Q

D

A

A

E

E

R

K

D

K

G

G

R

L

L

I

G

S

-

P

K

E

D

K

G

T

R

T

I

L

V

I

E

E

A

P

T

T

S

S

G

G

N

N

T

M

G

G

I

I

A

S

L

M

A

A

Q

F

I

M

A

A

V

A

L

M

K

K

G

G

Y

Y

A

K

I

M

T

V

I

L

V

T

M

M

P

P

E

S

G

Y

V

T

S

S

G

M

E

E

R

R

V

R

S

V

H

T

L

M

K

R

A

A

L

F

G

G

A

A

E

D

V

L

L

I

L

L

T

T

P

D

S

P

R

T

E

K

G

G

M

M

V

G

G

G

A

T

I

V

G

K

K

K

A

A

T

Y

E
|
E

M

L

E

L

K

E

S

S

E

T

K

P

G

N

L

A

F

F

M

M

P

L

R

Q

Q

Q

F

F

E

S

N

N

P

P

S

A

N

N

P

T

E

Q

S

V

H

H

Y

F

R

D

T

T

G

G

P

P

E

E

I

I

F

W

R

E

Q

E

-

T

L

L

G

G

R

N

V

V

P

-

D

D

G

I

F

F

V

V

A

M

G

G

V

I

G

G

T

S

G

G

T

T

I

V

S

T

G

G

V

V

G

G

R

L

Y

Y

L

L

R

K

E

S

K

K

K

N

R

P

D

N

L

V

K

K

I

I

W

Y

A

G

L

L

E

E

P

P

A

T

A

E

S

S

P

N

V

I

L

L

S

N

G

G

S

K

P

P

G

G

P

P

H

H

R

H

I

I

Q

T

G

G

I

N

G

G

A

V

G

G

F

S

E

K

P

P

R

D

T

I

F

V

N

D

R

M

S

D

V

L

V

M

D

E

R

E

I

V

E

L

K

M

I

V

S

S

D

S

R

E

E

D

A

A

I

V

D

N

M

M

A

A

R

R

R

E

L

L

S

A

Q

V

E

K

E

E

G

G

I

L

M

M

A

V

G

G

I

I

T

S

S

S

G

G

A

A

N

N

V

T

V

V

G

A

A

A

L

L

R

R

L

L

A

A

G

Q

E

K

L

P

P

E

P

N

G

K

S

G

H

K

V

L

V

I

T

V

I

T

V

V

C

H

D

A

S

S

F

F

-

G

E

E

R

R

Y

Y

F

L

S

S

M

S

E

V

K

L

Y

Y

L

Q

N

D

L

L

E157 (= E136) mutation E->N,Q: No effect on catalytic activities.

8b9yC Cysteine synthase from trypanosoma cruzi with plp and oas (see paper)
46% identity, 99% coverage: 4:312/312 of query aligns to 6:315/330 of 8b9yC

query
sites
8b9yC

P

P

E

R

G

N

D

N

V

V

I

A

R

P

N

S

P

M

L

D

A

A

L

L

I

I

G

G

N

E

T

T

P

P

L

A

L

V

P

Y

L

L

S

K

K

R

V

M

T

N

A

-

G

D

L

T

D

A

R

A

T

T

V

I

W

V

V

L

K

K

L

L

E

E

S

C

R

E

N

N

L

P

G

M

G

A

S

S

V

V

K
|
K

D

D

R

R

P

L

A

A

L

Y

F

A

M

I

I

Y

E

D

Q

K

A

A

E

E

R

K

D

E

G

G

R

K

L

I

-

I

-

P

G

G

K

K

D

S

G

-

R

V

I

I

V

V

E

E

A

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

Q

H

I

I

A

G

V

T

L

I

K

R

G

G

Y

Y

A

K

I

V

T

I

I

I

V

V

M

M

P

P

E

E

G

S

V

M

S

S

G

I

E

E

R

R

V

R

S

C

H

L

L

M

K

R

A

I

L

F

G

G

A

A

E

E

V

V

L

I

L

L

T

T

P

P

S

A

R

A

E

L

G

G

M

M

V

K

G

G

A

A

I

L

G

E

K

A

A

V

T

N

E

R

M

I

E

V

K

S

S

N

E

N

K

P

G

D

L

A

F

V

M

S

P

A

R

N

Q

Q

F

F

E

A

N

T

P

K

S

Y

N

N

P

A

E

Q

S

I

H

H

Y

E

R

E

T

T

G

G

P

P

E

E

I

I

F

W

R

R

Q

Q

L

T

G

K

R

G

V

H

P

V

D

D

G

C

F

F

V

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

V

V

G

A

R

R

Y

Y

L

L

R

K

E

S

K

V

K

G

R

C

D

G

L

A

K

T

I

I

W

F

A

A

L

V

E

E

P

P

A

A

A

E

S

S

P

P

V

V

L

L

S

S

G

G

S

K

P

P

G

G

P

P

H

H

R

R

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

E

V

P

P

R

E

T

V

F

F

N

E

R

A

S

A

V

L

V

V

D

D

R

E

I

V

E

I

K

Q

I

V

S

S

D

G

R

D

E

E

A

A

I

I

D

D

M

T

A

A

R

Q

R

K

L

L

S

P

Q

R

E

T

E

D

G

G

I

I

M

F

A

C

G

G

I

F

T
x
S

S

G

G

G

A

A

N

N

V

V

V

Y

G

A

A

A

L

L

R

Q

L

I

A

A

G

K

-

R

-

P

E

E

L

M

P

-

P

A

G

G

S

K

H

T

V

I

V

V

T

T

I

V

V

I

C
x
P

D
x
S

S

Y

F

G

E

E

R

R

Y

Y

F

L

S

S

M

T

E

A

K

L

Y

Y

L

S

N

S

L

I

binding pyridoxal-5'-phosphate: K50 (= K49), N81 (= N79), G185 (= G183), T186 (= T184), G187 (= G185), T189 (= T187), G229 (= G227), S273 (≠ T271), P300 (≠ C297), S301 (≠ D298)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
45% identity, 96% coverage: 8:305/312 of query aligns to 3:302/320 of 1z7yA

query
sites
1z7yA

V

I

I

A

R

K

N

D

P

V

L

T

A

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

L

V

P

Y

L

L

S

N

K

N

V

V

T

A

A

E

G

G

L

C

D

V

R

G

T

R

V

V

W

A

V

A

K

K

L

L

E

E

S

M

R

M

N

E

L

P

G

C

G

S

S

S

V

V

K
x
A

D

D

R

R

P

I

A

G

L

F

F

S

M

M

I

I

E

S

Q

D

A

A

E

E

R

K

D

K

G

G

R

L

L

I

G

K

K

P

-

G

D

E

G

S

R

V

I

L

V

I

E

E

A

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

Q

F

I

T

A

A

V

A

L

A

K

K

G

G

Y

Y

A

K

I

L

T

I

I

I

V

T

M

M

P

P

E

A

G

S

V

M

S

S

G

T

E

E

R

R

V

R

S

I

H

I

L

L

K

L

A

A

L

F

G

G

A

V

E

E

V

L

L

V

L

L

T

T

P

D

S

P

R

A

E

K

G

G

M

M

V

K

G

G

A

A

I

I

G

A

K

K

A

A

T

E

E

E

M

I

E

L

K

A

S

K

E

T

K

P

G

N

L

G

F

Y

M

M

P

L

R

Q

Q
|
Q

F

F

E

E

N

N

P

P

S

A

N

N

P

P

E

K

S

I

H

H

Y

Y

R

E

T

T

G

G

P

P

E

E

I

I

F

W

R

K

Q

G

L

T

G

G

R

G

V

K

P

I

D

D

G

G

F

F

V

V

A

S

G

G

V
x
I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

V

A

G

G

R

K

Y

Y

L

L

R

K

E

E

K

Q

K

N

R

A

D

N

L

V

K

K

I

L

W

Y

A

G

L

V

E

E

P

P

A

V

A

E

S

S

P

A

V

I

L

L

S

S

G

G

S

K

P

P

G

G

P

P

H

H

R

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

E

I

P

P

R

S

T

V

F

L

N

N

R

V

S

D

V

L

V

I

D

D

R

E

I

V

E

V

K

Q

I

V

S

S

D

S

R

D

E

E

A

S

I

I

D

D

M

M

A

A

R

R

R

Q

L

L

S

A

Q

L

E

K

E

E

G

G

I

L

M

L

A

V

G

G

I

I

T
x
S

S

S

G

G

A

A

N

A

V

A

G

A

A

A

L

I

R

K

L

L

A

A

G

Q

E

R

L

P

P

E

P

N

G

A

S

G

H

K

V

L

V

F

T

V

I

A

V

I

C

F

D
x
P

S
|
S

F

F

-

G

E

E

R

R

Y

Y

F

L

S

S

active site: A44 (≠ K49), S267 (≠ T271)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G78), N75 (= N79), T76 (= T80), Q145 (= Q149), I178 (≠ V182), G179 (= G183), T180 (= T184), G181 (= G185), T183 (= T187), G223 (= G227), S267 (≠ T271), P294 (≠ D298), S295 (= S299)

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
45% identity, 93% coverage: 15:305/312 of query aligns to 18:310/329 of 3vbeC

query
sites
3vbeC

L

L

I

I

G

G

N

R

T

T

P

P

L

L

L

V

P

Y

L

L

S

N

K

K

V

V

T

T

A

E

G

G

L

C

D

G

R

A

T

Y

V

V

W

A

V

V

K

K

L

Q

E

E

S

M

R

M

N

Q

L

P

G

T

G

A

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

Y

F

A

M

M

I

I

E

T

Q

D

A

A

E

E

R

E

D

K

G

N

R

L

L

I

-

T

-

P

G

G

K

K

D

T

G

-

R

T

I

L

V

I

E

E

A

P

T

T

S
|
S

G

G

N
|
N

T

M

G

G

I

I

A

S

L

M

A

A

Q

F

I

M

A

A

V

A

L

M

K

K

G

G

Y

Y

A

K

I

M

T

V

I

L

V

T

M

M

P

P

E

S

G

Y

V

T

S

S

G

L

E

E

R

R

V

R

S

V

H

T

L

M

K

R

A

A

L

F

G

G

A

A

E

E

V

L

L

I

L

L

T

T

P

D

S

P

R

A

E

K

G

G

M

M

V

G

G

G

A

T

I

V

G

K

K

K

A

A

T

Y

E

E

M

L

E

L

K

E

S

N

E

T

K

P

G

N

L

A

F

H

M

M

P

L

R

Q

Q

Q

F

F

E

S

N

N

P

P

S

A

N

N

P

T

E

Q

S

V

H

H

Y

F

R

E

T

T

G

G

P

P

E

E

I

I

F

W

R

E

Q

D

L

T

G

N

R

G

V

Q

P

V

D

D

G

I

F

F

V

V

A
x
M

G

G

V

I

G
|
G

T
x
S

G
|
G

G

G

T
|
T

I

V

S

S

G

G

V

V

G

G

R

Q

Y

Y

L

L

R

K

E

S

K

K

K

N

R

P

D

N

L

V

K

K

I

I

W

Y

A

G

L

V

E
|
E

P

P

A

S

A

E

S
|
S

P

N

V

V

L

L

S

N

G

G

S

K

P

P

G

G

P

P

H

H

R

H

I

I

Q

T

G
|
G

I

N

G

G

A

V

G

G

F

F

E

K

P

P

R

D

T

I

F

L

N

D

R

L

S

D

V

V

V

M

D

E

R

K

I

V

E

L

K

E

I

V

S

S

D

S

R

E

E

D

A

A

I

V

D

N

M

M

A

A

R

R

R

V

L

L

S

A

Q

L

E

K

E

E

G

G

I

L

M

M

A

V

G

G

I

I

T
x
S

S

S

G

G

A

A

N

N

V

T

V

V

G

A

A

A

L

L

R

R

L

L

A

A

G

-

E

Q

L

L

P

P

-

E

-

N

P

K

G

G

S

K

H

L

V

I

V

V

T

T

I

V

V

H

C
x
P

D

S

S

F

F

G

E

E

R

R

Y

Y

F

L

S

S

active site: K52 (= K49), S81 (= S77), E212 (= E208), S216 (= S212), S275 (≠ T271), P302 (≠ C297)
binding pyridoxal-5'-phosphate: K52 (= K49), N83 (= N79), M184 (≠ A180), G187 (= G183), S188 (≠ T184), G189 (= G185), T191 (= T187), G231 (= G227), S275 (≠ T271), P302 (≠ C297)

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
44% identity, 93% coverage: 16:305/312 of query aligns to 8:288/294 of 2bhtA

query
sites
2bhtA

I

I

G

G

N

N

T

T

P

P

L

L

L

V

P

K

L

L

S

Q

K

R

V

M

T

G

A

P

G

D

L

N

D

G

R

S

T

E

V

V

W

W

V

L

K

K

L

L

E

E

S

G

R

N

N

N

L

P

G

A

G

G

S

S

V

V

K
|
K

D

D

R

R

P

A

A

A

L

L

F

S

M

M

I

I

E

V

Q

E

A

A

E

E

R

K

D

R

G

G

R

R

L

I

G

K

K

P

D

G

G

D

R

V

I

L

V

I

E

E

A

A

T

T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

Q

M

I

I

A

A

V

A

L

L

K

K

G

G

Y

Y

A

R

I

M

T

K

I

L

V

L

M

M

P

P

E

D

G

N

V

M

S

S

G

Q

E

E

R

R

V

R

S

A

H

A

L

M

K

R

A

A

L

Y

G

G

A

A

E

E

V

L

L

I

L

L

T

V

P

T

S

K

R

E

E

Q

G

G

M

M

V

E

G

G

A

A

I

R

G

D

K

L

A

A

T

L

E

E

M

M

E

A

K

N

S

R

E

G

K

E

G

G

L

K

F

L

M

L

P

D

R

-

Q

Q

F

F

E

N

N

N

P

P

S

D

N

N

P

P

E

K

S

A

H

H

Y

Y

R

T

T

T

G

G

P

P

E

E

I

I

F

W

R

Q

Q

Q

L

T

G

G

R

G

V

R

P

I

D

T

G

H

F

F

V

V

A

S

G

S

V
x
M

G
|
G

T
|
T

G
x
T

G

G

T
|
T

I

I

S

T

G

G

V

V

G

S

R

E

Y

F

L

M

R

R

E

E

K

Q

K

S

R

K

D

P

L

V

K

T

I

I

W

V

A

G

L

L

E
x
Q

P

P

A

E

S
x
G

P

S

V

S

L

I

S

P

G
|
G

G

I

S

R

P

R

G

W

P

P

H

T

R

E

I

-

Q

-

G

-

I

-

G

-

A

-

G

-

F

Y

E

L

P

P

R

G

T

I

F

F

N

N

R

A

S

S

V

L

V

V

D

D

R

E

I

V

E

L

K

D

I

I

S

H

D

Q

R

R

E

D

A

A

I

E

D

N

M

T

A

M

R

R

R

E

L

L

S

A

Q

V

E

R

E

E

G

G

I

I

M

F

A

C

G

G

I

V

T
x
S

S

S

G

G

A

G

N

A

V

V

V

A

G

G

A

A

L

L

R

R

L

V

A

A

G

-

E

K

L

A

P

N

P

P

G

D

S

A

H

V

V

V

T

A

I

I

V

I

C
|
C

D

D

S

R

F

G

E

D

R

R

Y

Y

F

L

S

S

active site: K41 (= K49), S69 (= S77), Q199 (≠ E208), G203 (≠ S212), S255 (≠ T271), C280 (= C297)
binding pyridoxal-5'-phosphate: K41 (= K49), N71 (= N79), M173 (≠ V182), G174 (= G183), T175 (= T184), T176 (≠ G185), T178 (= T187), G208 (= G217), S255 (≠ T271), C280 (= C297)

Query Sequence

>WP_014449906.1 NCBI__GCF_000284315.1:WP_014449906.1
MTSPEGDVIRNPLALIGNTPLLPLSKVTAGLDRTVWVKLESRNLGGSVKDRPALFMIEQA
ERDGRLGKDGRIVEATSGNTGIALAQIAVLKGYAITIVMPEGVSGERVSHLKALGAEVLL
TPSREGMVGAIGKATEMEKSEKGLFMPRQFENPSNPESHYRTTGPEIFRQLGRVPDGFVA
GVGTGGTISGVGRYLREKKRDLKIWALEPAASPVLSGGSPGPHRIQGIGAGFEPRTFNRS
VVDRIEKISDREAIDMARRLSQEEGIMAGITSGANVVGALRLAGELPPGSHVVTIVCDSF
ERYFSMEKYLNL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory