SitesBLAST
Comparing WP_014625858.1 NCBI__GCF_000013085.1:WP_014625858.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6uecA Pseudomonas aeruginosa lpxd complex structure with ligand (see paper)
35% identity, 97% coverage: 5:362/369 of query aligns to 2:337/337 of 6uecA
3pmoA The structure of lpxd from pseudomonas aeruginosa at 1.3 a resolution (see paper)
35% identity, 97% coverage: 5:362/369 of query aligns to 22:357/357 of 3pmoA
Sites not aligning to the query:
P0CD76 UDP-3-O-acylglucosamine N-acyltransferase; EC 2.3.1.191 from Chlamydia trachomatis serovar D (strain ATCC VR-885 / DSM 19411 / UW-3/Cx) (see paper)
38% identity, 70% coverage: 107:365/369 of query aligns to 105:347/354 of P0CD76
- D240 (= D258) binding hexadecanoate
- Q244 (≠ M262) binding hexadecanoate
- Q248 (≠ N266) binding UDP-N-acetyl-alpha-D-glucosamine
- S266 (= S284) binding UDP-N-acetyl-alpha-D-glucosamine
- H284 (= H302) binding UDP-N-acetyl-alpha-D-glucosamine
2iu8A Chlamydia trachomatis lpxd with 25mm udpglcnac (complex i) (see paper)
38% identity, 70% coverage: 107:364/369 of query aligns to 105:346/346 of 2iu8A
- binding palmitic acid: D240 (= D258), Q244 (≠ M262), A246 (≠ G264), A264 (= A282), G280 (= G298), T282 (≠ A300), A295 (= A313), Q296 (≠ G314), T300 (≠ G318)
- binding uridine-diphosphate-n-acetylglucosamine: F190 (= F192), G191 (≠ S193), H247 (= H265), Q248 (≠ N266), G265 (= G283), G283 (≠ D301), H284 (= H302)
4ihhE Chasing acyl carrier protein through a catalytic cycle of lipid a production (see paper)
27% identity, 97% coverage: 9:365/369 of query aligns to 7:334/340 of 4ihhE
P21645 UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase; UDP-3-O-(3-OHC14)-GlcN N-acyltransferase; Protein FirA; Rifampicin resistance protein; UDP-3-O-(3-hydroxytetradecanoyl)glucosamine N-acyltransferase; EC 2.3.1.191 from Escherichia coli (strain K12) (see 4 papers)
27% identity, 97% coverage: 9:365/369 of query aligns to 6:333/341 of P21645
- F41 (≠ V48) mutation to A: 30-fold decrease in affinity for UDP-3-O-((3R)-hydroxytetradecanoyl)-GlcN and 5-fold decrease in catalytic activity.
- Q165 (≠ R174) mutation to R: In firA200; confers temperature sensitivity and reverses the rifampicin resistance of rpoB mutants.
- G228 (= G254) mutation to D: In firA200 and firA201; confers temperature sensitivity.
- H239 (= H265) active site, Proton acceptor; mutation to A: 1000-fold reduction in catalytic activity with very little effect on substrate affinity.
- G252 (≠ Q278) mutation to S: In firA200 and firA201; confers temperature sensitivity.
- S271 (≠ V297) mutation to N: In omsA; confers temperature sensitivity.
- H276 (= H302) mutation to A: 30-fold reduction in catalytic activity.
- M290 (≠ S315) mutation to A: Change in the acyl donor selectivity, showing a preference for 3-hydroxypalmitoyl-ACP over 3-hydroxytetradecanoyl-ACP as substrate. Produces a lipid A with longer acyl chains.
- M292 (≠ V317) mutation to A: No change in the acyl donor selectivity.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
6p8aA E.Coli lpxd in complex with compound 8.1 (see paper)
27% identity, 97% coverage: 9:365/369 of query aligns to 4:331/335 of 6p8aA
- binding 3-hydroxy-7,7-dimethyl-2-phenyl-4-(thiophen-2-yl)-2,6,7,8-tetrahydro-5H-pyrazolo[3,4-b]quinolin-5-one: F181 (= F192), I252 (≠ G280), A254 (= A282)
- binding 2-[4,6-dimethyl-3-(1H-pyrrol-1-yl)-1H-pyrazolo[3,4-b]pyridin-1-yl]-N-[3-(morpholin-4-yl)propyl]acetamide: A246 (≠ M274), A248 (≠ C276), M264 (≠ V292), I265 (≠ L293), G266 (≠ A294), T284 (≠ V311), G285 (= G312)
6p89A E.Coli lpxd in complex with compound 7 (see paper)
27% identity, 97% coverage: 9:365/369 of query aligns to 4:331/335 of 6p89A
- binding 3-hydroxy-7,7-dimethyl-2-phenyl-4-(thiophen-2-yl)-2,6,7,8-tetrahydro-5H-pyrazolo[3,4-b]quinolin-5-one: F181 (= F192), I252 (≠ G280), A254 (= A282)
- binding N-(2,3-dihydro-1,4-benzodioxin-6-yl)-2-(8-methyl[1]benzopyrano[4,3-c]pyrazol-1(4H)-yl)acetamide: M264 (≠ V292), I265 (≠ L293), G266 (≠ A294), T284 (≠ V311), G285 (= G312)
6p88A E.Coli lpxd in complex with compound 6 (see paper)
27% identity, 97% coverage: 9:365/369 of query aligns to 4:331/335 of 6p88A
- binding 3-hydroxy-7,7-dimethyl-2-phenyl-4-(thiophen-2-yl)-2,6,7,8-tetrahydro-5H-pyrazolo[3,4-b]quinolin-5-one: F181 (= F192), Y183 (≠ F194), I252 (≠ G280), A254 (= A282)
- binding N-{3-[(furan-2-carbonyl)amino]phenyl}-2,3-dihydro-1,4-benzodioxine-6-carboxamide: A248 (≠ C276), M264 (≠ V292), G285 (= G312)
6p87A E.Coli lpxd in complex with compound 5 (see paper)
27% identity, 97% coverage: 9:365/369 of query aligns to 4:331/335 of 6p87A
- binding 3-hydroxy-7,7-dimethyl-2-phenyl-4-(thiophen-2-yl)-2,6,7,8-tetrahydro-5H-pyrazolo[3,4-b]quinolin-5-one: F181 (= F192), I252 (≠ G280), A254 (= A282)
- binding N-[(R)-(1H-indol-3-yl)(thiophen-2-yl)methyl]-2-(2-methoxyphenoxy)-N-methylacetamide: A248 (≠ C276), M264 (≠ V292), G266 (≠ A294), G267 (= G295), T284 (≠ V311), G285 (= G312)
6p86A E.Coli lpxd in complex with compound 4.1 (see paper)
27% identity, 97% coverage: 9:365/369 of query aligns to 4:331/335 of 6p86A
- binding 3-hydroxy-7,7-dimethyl-2-phenyl-4-(thiophen-2-yl)-2,6,7,8-tetrahydro-5H-pyrazolo[3,4-b]quinolin-5-one: F181 (= F192), I252 (≠ G280), A254 (= A282)
- binding N-[(4-ethylphenyl)methyl]-2-[(6aR)-6-oxo-3-(pyrrolidine-1-carbonyl)-6,6a,7,8,9,10-hexahydro-5H-pyrido[1,2-a]quinoxalin-5-yl]acetamide: A246 (≠ M274), A248 (≠ C276), G266 (≠ A294), G267 (= G295), T284 (≠ V311), G285 (= G312)
6p85A E.Coli lpxd in complex with compound 3 (see paper)
27% identity, 97% coverage: 9:365/369 of query aligns to 4:331/335 of 6p85A
- binding 3-hydroxy-7,7-dimethyl-2-phenyl-4-(thiophen-2-yl)-2,6,7,8-tetrahydro-5H-pyrazolo[3,4-b]quinolin-5-one: F181 (= F192), I252 (≠ G280), A254 (= A282)
- binding 1-(2-ethoxyphenyl)-4-[(4-methylphenyl)sulfonyl]-1H-1,2,3-triazol-5-amine: A248 (≠ C276), G266 (≠ A294), G267 (= G295), G285 (= G312)
6p84A E.Coli lpxd in complex with compound 2o (see paper)
27% identity, 97% coverage: 9:365/369 of query aligns to 4:331/335 of 6p84A
- binding 3-hydroxy-7,7-dimethyl-2-phenyl-4-(thiophen-2-yl)-2,6,7,8-tetrahydro-5H-pyrazolo[3,4-b]quinolin-5-one: F181 (= F192), A248 (≠ C276), I252 (≠ G280), A254 (= A282), M264 (≠ V292), I265 (≠ L293), G266 (≠ A294), G267 (= G295)
6p83A E.Coli lpxd in complex with compound 1o (see paper)
27% identity, 97% coverage: 9:365/369 of query aligns to 4:331/335 of 6p83A
- binding 4-(2-chlorophenyl)-3-hydroxy-7,7-dimethyl-2-phenyl-2,6,7,8-tetrahydro-5H-pyrazolo[3,4-b]quinolin-5-one: F181 (= F192), A248 (≠ C276), I252 (≠ G280), A254 (= A282), M264 (≠ V292), I265 (≠ L293), G266 (≠ A294), G267 (= G295), V283 (= V310)
4ihgE Chasing acyl carrier protein through a catalytic cycle of lipid a production (see paper)
27% identity, 97% coverage: 9:365/369 of query aligns to 5:332/337 of 4ihgE
P0A1X4 UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase; UDP-3-O-(3-OHC14)-GlcN N-acyltransferase; UDP-3-O-(3-hydroxytetradecanoyl)glucosamine N-acyltransferase; EC 2.3.1.191 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
28% identity, 97% coverage: 9:365/369 of query aligns to 6:333/341 of P0A1X4
- V291 (≠ G316) mutation to M: In SS-C; sensitive to hydrophobic antibiotics and compounds.
4ihfA Chasing acyl carrier protein through a catalytic cycle of lipid a production (see paper)
27% identity, 97% coverage: 9:365/369 of query aligns to 4:331/336 of 4ihfA
- active site: A237 (≠ H265), N238 (= N266), G255 (= G283)
- binding S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] (3R)-3-hydroxytetradecanethioate: F181 (= F192), D230 (= D258), A236 (≠ G264), G249 (≠ A277), I252 (≠ G280), A254 (= A282), G267 (= G295), V270 (≠ G298), N272 (≠ A300), T284 (≠ V311), G285 (= G312), V289 (≠ G316), N308 (≠ K335), R312 (= R346)
4eqyG Crystal structure of acyl-[acyl-carrier-protein]--udp-n- acetylglucosamine o-acyltransferase from burkholderia thailandensis (see paper)
30% identity, 61% coverage: 109:334/369 of query aligns to 2:188/260 of 4eqyG
6p9tA E.Coli lpxa in complex with udp-3-o-(r-3-hydroxymyristoyl)-glcnac and compound 8 (see paper)
27% identity, 61% coverage: 109:334/369 of query aligns to 8:194/263 of 6p9tA
- active site: H125 (= H265), D126 (≠ N266), G143 (= G283)
- binding [3-(5-amino-4-methylpyridin-2-yl)-1H-pyrazol-5-yl]{(3R)-3-[(2-chloro-6-methoxyphenyl)methyl]morpholin-4-yl}methanone: H160 (≠ A300), H191 (≠ P331)
- binding uridine-5'-diphosphate-3-O-(R-3-hydroxymyristoyl)-N-acetyl-D-glucosamine: D74 (≠ R217), L75 (= L218), K76 (≠ A219), H122 (≠ M262), A124 (≠ G264), H125 (= H265), A142 (= A282), G143 (= G283), H144 (≠ S284), A158 (≠ G298), Q161 (≠ D301)
6p9sA E.Coli lpxa in complex with udp-3-o-(r-3-hydroxymyristoyl)-glcnac and compound 7 (see paper)
27% identity, 61% coverage: 109:334/369 of query aligns to 8:194/263 of 6p9sA
- active site: H125 (= H265), D126 (≠ N266), G143 (= G283)
- binding {(3R)-3-[(2-methoxyphenyl)methyl]morpholin-4-yl}[3-(4-methylpyridin-2-yl)-1H-pyrazol-5-yl]methanone: H160 (≠ A300), H191 (≠ P331)
- binding uridine-5'-diphosphate-3-O-(R-3-hydroxymyristoyl)-N-acetyl-D-glucosamine: D74 (≠ R217), L75 (= L218), K76 (≠ A219), H122 (≠ M262), A124 (≠ G264), H125 (= H265), T140 (≠ G280), A142 (= A282), G143 (= G283), H144 (≠ S284), A158 (≠ G298), Q161 (≠ D301), H191 (≠ P331)
Query Sequence
>WP_014625858.1 NCBI__GCF_000013085.1:WP_014625858.1
MTQPLPMTLTQVAEALGGTLHGQGDLIIRRPVHPARAEAGEGVTDLAVAMEPALLAALEG
SAARAALVAGGAVPPAGSVDGWIEVGRPRFAMHVITGRFEQPARLAPGIHPSAVVEEGAE
IGEGAALGPFVHVGFGARVGAGSRVHSGVSIGAGAVVGADCLLHPGVRIGERVRVGDRVI
LHANAVIGADGFSFVTPEPGSVESAKATGRVDAINSRLARIASLGAVVLGDDVEIGANTC
IDRGTLDDTRIGDGTKIDDMVMIGHNVRVGRLCMLCAQVGIAGSAVIGDGVVLAGRVGVA
DHITIGDNAVVGAGSGVGSNIPPRSVWMGYPALPKDQATEHYLFSRRLKHLFKDVSELKK
RIRSLTVPS
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory