SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_015448129.1 NCBI__GCF_000230695.2:WP_015448129.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
40% identity, 98% coverage: 1:457/465 of query aligns to 1:452/457 of P15993

query
sites
P15993

M

M

T

M

A

E

S

G

L

Q

E

Q

H

H

P

G

E

E

H

Q

L

L

R

K

R

R

S

G

L

L

S

K

N

N

R

R

H

H

L

I

Q

Q

L

L

I

I

A

A

I

L

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

F

M

L

G

G

S

S

G

A

K

S

T

V

I

I

S

Q

L

S

A

A

G

G

P

P

S

G

I

I

L

I

L

L

V

G

Y

Y

L

A

I

I

I

A

G

G

V

F

M

I

L

A

F

F

F

L

V

I

M

M

R

R

A

Q

M

L

G

G

E

E

L

M

L

V

S

E

N

E

L

P

E

V

Y

A

K

G

S

S

F

F

I

S

D

H

F

F

S

A

T

Y

D

K

L

Y

L

W

G

G

P

S

W

F

A

A

G

G

F

F

F

A

C

S

G

G

W

W

T

N

Y

Y

W

W

F

V

C

L

W
x
Y

V

V

I

L

T

V

A

A

I

M

A

A

D

E

V

L

I

T

A

A

I

V

A

G

A

K

Y

Y

A

I

Q

Q

F

F

W

W

F

Y

P

P

G

E

L

I

A

P

P

T

W

W

I

V

P

S

A

A

V

A

L

V

C

F

V

F

L

V

L

I

L

N

S

A

L

I

N

N

L

L

V

T

T

N

V

V

K

K

L

V

F

F

G

G

E

E

L

M

E

E

F

F

W

W

F

F

A

A

L

I

I

I

K

K

I

V

V

I

A

A

I

V

V

V

A

A

L

M

I

I

A

I

T

F

G

G

F

G

A

W

L

L

V

L

A

-

W

-

G

-

F

F

T

S

S

G

P

N

S

G

G

G

H

P

K

Q

A

A

S

T

L

V

A

S

N

N

L

L

W

W

S

D

D

Q

G

G

F

F

F

L

P

P

K

H

G

G

L

F

S

T

G

G

F

L

F

V

A

M

G

M

F

M

Q

A

I

I

A

I

V

M

F

F

A

S

F

F

V

G

G

G

I

L

E

E

L

L

V

V

G

G

T

I

T

T

A

A

E

E

T

A

A

D

D

N

P

P

K

E

R

Q

N

S

L

I

P

P

K

K

A

A

I

T

N

N

S

Q

I

V

P

I

V

Y

R

R

I

I

I

L

I

I

F

F

Y

Y

V

I

L

G

A

S

L

L

I

A

A

V

I

L

M

L

V

S

V

L

T

M

P

P

W

W

R

T

L

R

V

V

E

T

A

A

G

D

K

T

S

S

P

P

F

F

V

V

E

L

L

I

F

F

V

H

L

E

A

L

G

G

I

D

P

T

A

F

A

V

A

A

S

N

L

A

I

L

N

N

F

I

V

V

L

L

T

T

S

A

A

A

T

L

S

S

S

V

A

Y

N

N

S

S

G

C

I

V

F

Y

S

C

T

N

S

S

R

R

M

M

L

L

Y

F

G

G

L

L

A

A

E

Q

Q

G

H

N

A

A

P

P

K

K

T

A

F

L

A

A

R

S

L

V

S

D

R

K

A

R

A

G

V

V

P

P

S

V

L

N

G

T

L

I

L

L

F

V

S

S

C

A

F

L

C

V

L

T

L

A

L

L

G

C

A

V

A

L

M

I

I

N

Y

Y

L

L

I

A

P

P

D

E

L

-

I

-

T

S

A

A

F

F

T

G

L

L

I

L

T

M

T

A

L

L

S

V

A

V

V

S

L

A

F

L

M

V

F

I

V

N

W

W

S

A

L

M

I

I

L

S

F

L

G

A

Y

H

I

M

A

K

Y

F

R

R

Q

A

R

K

P

Q

Q

E

L

-

H

-

Q

Q

A

G

S

V

I

V

Y

T

K

R

M

F

P

P

G

A

G

L

V

L

F

Y

-

P

-

L

M

G

C

N

W

W

A

I

C

C

L

L

A

L

F

F

F

M

V

A

F

A

V

V

L

L

V

V

L

I

L

M

S

L

L

M

Q

T

P

P

D

G

T

M

R

A

E

I

A

S

L

V

I

Y

A

L

S

I

P

P

V

V

W

W

F

L

V

I

L

V

L

L

G

G

I

I

G

G

Y

Y

L

L

W

F

K

K

G

E

R

K

R

T

A

A

R

K

Y103 (≠ W103) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P24207 Phenylalanine-specific permease; Phenylalanine:H(+) symporter PheP from Escherichia coli (strain K12) (see 3 papers)
40% identity, 95% coverage: 2:445/465 of query aligns to 11:448/458 of P24207

query
sites
P24207

T

T

A

A

S

S

L

N

E

Q

H

E

P

P

E

T

H

-

L

L

R

H

R

R

S

G

L

L

S

H

N

N

R
|
R

H

H

L

I

Q

Q

L

L

I

I

A

A

I

L

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

F

M

L

G

G

S

I

G

G

K

P

T

A

I

I

S

Q

L

M

A

A

G

G

P
|
P

S

A

I

V

L

L

V

G

Y

Y

L

G

I

V

I

A

G

G

V

I

M

I

L

A

F

F

F

L

V

I

M

M

R

R

A

Q

M

L

G

G

E

E

L

M

L

V

S

E

N

E

L

P

E

V

Y

S

K

G

S

S

F
|
F

I

A

D

H

F
|
F

S

A

T
x
Y

D

K

L
x
Y

L
x
W

G

G

P

P

W
x
F

A

A

G

G

F
|
F

F

L

C

S

G

G

W
|
W

T

N

Y
|
Y

W
|
W

F

V

C

M

W
x
F

V

V

I

L

T

V

A

G

I

M

A

A

D
x
E

V

L

I

T

A

A

I

A

A

G

A

I

Y

Y

A

M

Q

Q

F

Y

W

W

F

F

P

P

G

D

L

V

A

P

P

T

W

W

I

I

P

W

A

A

V

A

L

A

C

F

V

F

L

I

L

N

S

A

L

V

N

N

L

L

V

V

T

N

V

V

K

R

L

L

F

Y

G

G

E

E

L

T

E

E

F

F

W

W

F

F

A

A

L

L

I

I

K
|
K

I

V

V

L

A

A

I

I

V

I

A

G

L

M

I

I

A

G

T

F

G

G

F

L

A

W

L

L

V

L

A

-

W

-

G

-

F

F

T

S

S

G

P

H

S

G

G

G

H

E

K

K

A

A

S

S

L

I

A

D

N

N

L

L

W

W

S

R

D

Y

G

G

F

F

P

A

K

T

G

G

L

W

S

N

G

G

F

L

F

I

A

L

G

S

F

L

Q

A

I

V

A

I

V

M

F

F

A

S

F

F

V

G

G

G

I

L

E
|
E

L

L

V

I

G

G

T

I

T

T

A

A

E

E

T

A

A

R

D

D

P

P

K

E

R

K

N

S

L

I

P

P

K

K

A

A

I

V

N

N

S

Q

I

V

P

V

V

Y

R
|
R

I

I

I

L

F

F

Y

Y

V

I

L

G

A

S

L

L

I

V

A

V

I

L

M

L

V

A

V

L

T

Y

P

P

W

W

R

V

L

E

V

V

E

K

A

S

G

N

K

S

S

S

P

P

F

F

V

V

E

M

L

I

F

F

V

H

L

N

A

L

G

D

I

S

P

N

A

V

A

A

S

S

L

A

I

L

N

N

F

F

V

V

V

I

L

L

T

V

S

A

A

S

T

L

S

S

S

V

A

Y

N

N

S

S

G

G

I

V

F

Y

S

S

T

N

S

S

R

R

M

M

L

L

Y

F

G

G

L

L

A

S

E

V

E

Q

Q

G

H

N

A

A

P

P

K

K

T

F

F

L

A

T

R

R

L

V

S

S

R

R

A

R

A

G

V

V

P
|
P

S

I

L

N

G

S

L

L

L

M

F

L

S

S

C

G

F

A

C

I

L

T

L

S

L

L

G

V

A

V

A

L

M

I

I

N

Y

Y

L

L

I

L

P

P

D

Q

L

-

I

-

T

K

A

A

F

F

T

G

L

L

I

L

T

M

T

A

L

L

S

V

A

V

V

A

L

T

F

L

M

L

F

L

V

N

W

W

S

I

L

M

I

I

L

C

F

L

G

A

Y

H

I

L

A

R

Y

F

R

R

R

A

Q

A

R

M

P

R

Q

R

L

Q

H

G

Q

R

A

E

S

T

I

Q

Y

F

K

K

M

A

P

L

G

L

G

Y

V

P

F

F

M

G

C

N

W

Y

A

L

C

C

L

I

A

A

F

F

F

L

V

G

F

M

V

I

L

L

V

L

L

L

L

M

S

C

L

T

Q

M

P

D

D

D

T

M

R

R

E

L

A

S

L

A

I

I

A

L

S

L

P
|
P

V

V

W

W

F

I

V

V

L

F

L

L

R26 (= R18) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P46) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (= F79) mutation to L: No effect on phenylalanine transport activity.
F90 (= F82) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ T84) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (≠ L86) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ L87) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ W90) mutation to L: No effect on phenylalanine transport activity.
F101 (= F93) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W97) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (= Y99) mutation to L: No effect on phenylalanine transport activity.
W108 (= W100) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ W103) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (≠ D110) mutation E->G,L,V,N: Loss of activity.
K168 (= K160) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E221) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R247) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P336) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.
P442 (= P439) mutation to A: 46% of wild-type phenylalanine transport activity.; mutation to G: 52% of wild-type phenylalanine transport activity.; mutation to L: 43% of wild-type phenylalanine transport activity.

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
35% identity, 98% coverage: 6:461/465 of query aligns to 3:456/469 of P46349

query
sites
P46349

E

Q

H

S

P

Q

E

S

H

G

L

L

R

K

S

E

L

L

S

K

N

T

R

R

H

H

L

M

Q

T

L

M

I

I

A

S

I

I

G

A

G

G

A

V

I

I

G

G

T

A

G

G

L

L

F

F

M

V

G
|
G

S

S

G

G

K

S

T

V

I

I

S

H

L

S

A

T

G
|
G

P

P

S

G

I

A

L

V

V

S

Y

Y

L

A

I

L

I

A

G

G

V

L

M

L

L

V

F

I

F

F

V

I

M

M

R

R

A

M

M

L

G

G

E

E

L

M

L

S

L

A

S

V

N

N

L

P

E

T

Y

S

K

G

S

S

F

F

I

S

D

Q

F

Y

S

A

T

H

D

D

L

A

L

I

G

G

P

P

W

W

A

A

G

G

F

F

F

T

C

I

G

G

W

W

T

L

Y

Y

W

W

F

F

C

F

W

W

V

V

I

I

T

V

A

I

I

A

A

I

D

E

V

A

I

I

A

A

I

G

A

A

A

G

Y

I

A

I

Q

Q

F

Y

W

W

F

F

P

H

G

D

L

I

A

P

P

L

W

W

I

L

P

T

A

S

V

L

L

I

C

L

V

T

L

I

L

V

L

L

L

T

S

L

L

T

N

N

L

V

V

Y

T

S

V

V

K

K

L

S

F

F

G

G

E

E

L

F

E

E

F

Y

W

W

F

F

A

S

L

L

I

I

K

K

I

V

V

V

A

T

I

I

V

I

A

A

L

F

I

L

A

I

T

V

G

G

F

F

A

A

L

F

V

I

A

-

W

F

G

G

F

F

T

-

S

A

P

P

S

G

G

S

H

E

K

P

A

V

S

G

L

F

A

S

N

N

L

L

W

T

S

G

D

K

G

G

F

F

P

P

K

E

G

G

L

I

S

S

G

S

F

V

F

L

A

L

G

G

F

I

Q

V

I

V

A

V

V

I

F

F

A

S

F

F

V

M

G

G

I

T

E

E

L

I

V

V

G

A

T

I

T

A

A

A

A

G

E

E

T

T

A

S

D

N

P

P

K

I

R

E

N

S

L

V

P

T

K

K

A

A

I

T

N

R

S

S

I

V

P

V

V

W

R

R

I

I

I

V

F

F

Y

Y

V

V

L

G

A

S

L

I

I

A

A

I

I

V

M

V

V

A

V

L

T

L

P

P

W

W

R

N

L

S

V

A

E

N

A

I

G

L

K

E

S

S

P

P

F

F

V

V

E

A

L

V

F

L

V

E

L

H

A

I

G

G

I

V

P

P

A

A

S

Q

L

I

I

M

N

N

F

F

V

I

V

V

L

L

T

T

S

A

A

V

T

L

S

S

S

C

A

L

N

N

S

S

G
|
G

I

L

F

Y

S

T

T

T

S

S

R

R

M

M

L

L

Y

Y

G

S

L

L

A

A

E

E

E

R

Q

N

H

E

A

A

P

P

K

R

T

R

F

F

A

M

R

K

L

L

S

S

R

K

A

K

A

G

V

V

P

P

S

V

L

Q

G

A

L

I

L

V

F

A

S
x
G

C

T

F

F

C
x
F

L

S

L

Y

L

I

G

A

A

V

M

M

I

N

Y

Y

L

F

I

S

P

P

D

D

L

-

I

-

T

T

A

V

F

F

T

L

L

F

I

L

T

V

T

N

L

S

S

S

A

G

V

A

L

I

F

A

M

L

F

L

V

V

W

Y

S

L

L

V

I

I

L

A

F

V

G

S

Y

Q

I

L

A

K

Y

M

R

R

R

K

Q

K

-

L

-

E

-

K

-

T

R

N

P

P

Q

E

L

A

H

L

Q

K

A

I

S

K

I

M

Y

W

K

L

M

F

P

P

G

-

V

-

F

F

M

L

C

T

W

Y

A

L

C

T

L

I

A

I

F

A

F

I

V

C

F
x
G

V

I

L

L

V

V

L

S

L

M

S

A

L

F

Q

I

P

D

D

S

T

M

R

R

E

D

A

E

L

L

I

L

A

L

S

T

P

G

V

V

W

I

F

T

V

G

L

I

L

V

G

L

I

I

G

S

Y

Y

L

L

-

V

W

F

K

R

G

K

R

R

R

K

A

V

R

S

R

E

G

K

V

A

A

A

G33 (= G36) mutation to D: Lack of activity.
G42 (= G45) mutation to S: Lack of activity.
G301 (= G306) mutation to V: Lack of activity.
G338 (≠ S343) mutation to E: Lack of activity.
F341 (≠ C346) mutation to S: Lack of activity.
G414 (≠ F420) mutation to R: Lack of activity.

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
37% identity, 76% coverage: 2:356/465 of query aligns to 5:372/489 of P25737

query
sites
P25737

T

T

A

K

S

T

L

T

E

E

H

A

P

P

E

-

H

G

L

L

R

R

S

E

L

L

S

K

N

A

R

R

H

H

L

L

Q

T

L

M

I

I

A

A

I

I

G

G

A

S

I

I

G

G

T

T

G

G

L

L

F

F

M

V

G

A

S

S

G

G

K

A

T

T

I

I

S

S

L

Q

A

A

G

G

P

P

S

G

-

G

I

A

L

L

V

S

Y

Y

L

M

I

L

I

I

G

G

V

L

M

M

L

V

F

Y

F

F

V

L

M

M

R

T

A

S

M

L

G

G

E

E

L

L

L

A

S

Y

N

M

L

P

E

V

Y

S

K

G

S

S

F

F

I

A

D

T

F

Y

S

G

T

Q

D

N

L

Y

L

V

G

E

P

E

W

G

A

F

G

G

F

F

F

A

C

L

G

G

W

W

T

N

Y
|
Y

W

W

F

Y

C

N

W
|
W

V

A

I

V

T

T

A

I

I

A

A

V

D

D

V

L

I

V

A

A

I

A

A

Q

A

L

Y

V

A

M

Q

S

F

W

W

W

F

F

P

P

G

D

L

T

A

P

P

G

W

W

I

I

P

W

A

S

V

A

L

L

C

F

V

L

L

G

L

V

L

I

L

F

S

L

L

L

N

N

L

Y

V

I

T

S

V

V

K

R

L

G

F

F

G

G

E

E

L

A

E

E

F

Y

W

W

F

F

A

S

L

L

I

I

K
|
K

I

V

V

T

A

T

I

V

V

I

A

V

L

F

I

I

A

I

T

V

G

G

F

V

A

L

L

M

V

I

A

I

W

G

G

I

F

F

T

K

S

-

P

-

S

G

G

A

H

Q

K

P

A

A

S

G

L

W

A

S

N

N

L

-

W

W

S

T

D

I

G

G

G

E

F

A

-

P

F

F

P

A

K

G

G

G

L

F

S

A

G

A

F

M

F

I

A

G

G

V

F

A

Q

M

I

I

A

V

V

G

F
|
F

A

S

F

F

V

Q

G

G

I

T

E
|
E

L

L

V

I

G

G

T

I

T

A

A

A

A

G

E
|
E

T

S

A

E

D

D

P

P

K

A

R

K

N

N

L

I

P

P

K

R

A

A

I

V

N

R

S

Q

I

V

P

F

V

W

R

R

I

I

I

L

F

F

Y

Y

V

V

L

F

A

A

L

I

I

L

A

I

I

I

M

S

V

L

V

I

T

I

P

P

W

Y

-

T

-

D

-

P

-

S

-

L

-

R

-

N

R
x
D

L

V

V

K

E
x
D

A

I

G

S

K

V

S

S

P

P

F

F

V

T

E

L

L

V

F

F

V

Q

L

H

A

A

G

G

I

L

P

L

A

S

A

A

S

A

L

V

I

M

N

N

F

A

V

V

V

I

L

L

T

T

S

A

A

V

T

L

S

S

S

A

A

G

N

N

S

S

G

G

I

M

F

Y

S

A

T

S

S

T

R

R

M

M

L

L

Y

Y

G

T

L

L

A

A

E

C

E

D

Q

G

H

K

A

A

P

P

K

R

T

I

F

F

A

A

R

K

L

L

S

S

R

R

A

G

V

V

P

P

S

R

L

N

G

A

L

L

L

Y

F

A

S

T

-

T

-

V

-

I

-

A

-

G

-

L

C

C

F

F

C

L

L

T

-

S

L

M

L

F

G

G

A

N

A

Q

M

T

I

V

Y

Y

L

L

Y102 (= Y99) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (= W103) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K160) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (= F215) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E221) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E229) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (≠ R266) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (≠ E269) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
32% identity, 91% coverage: 3:426/465 of query aligns to 72:503/587 of Q9URZ4

query
sites
Q9URZ4

A

A

S

T

L

G

E

E

H

D

P

G

E

T

H

A

L

L

R

K

R

R

S

S

L

L

S

K

N

S

R

R

H

H

L

M

Q

Q

L

M

I

I

A

S

I

I

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

Y

M

V

G

G

S

S

G

G

K

S

T

S

I

L

S

A

L

D

A

G

G

G

P

P

-

A

S

S

I

V

L

I

V

N

Y

Y

L

S

I

L

I

I

G

G

V

I

M

M

L

M

F

F

V

I

M

V

R

Y

A

A

M

L

G

G

E

E

L

M

L

A

L

V

S

A

N

Y

L

P

E

V

Y

A

K

G

S

G

F

F

I

N

D

T

F

Y

S

A

T

T

D

R

L

F

L

I

G

D

P

P

W

A

A

W

G

G

F

F

F

A

C

V

G

S

W

W

T

N

Y

Y

W

F

F

I

C

N

W

Y

V

F

I

V

T

T

A

F

I

P

A

L

D

E

V

L

I

T

A

T

I

C

A

A

A

I

Y

T

A

F

Q

R

F

Y

W

W

F

T

P

D

-

I

G

N

L

S

A

A

P

A

W

W

I

I

P

S

A

I

V

F

L

L

C

V

V

V

L

I

L

-

S

-

L

V

N

N

L

L

V

F

T

G

V

V

K

R

L

A

F

Y

G

G

E

E

L

V

E

E

F

F

W

I

F

L

A

S

L

T

I

V

K

K

I

V

V

V

A

A

I

T

V

F

A

G

L

F

I

I

A

I

T

L

G

A

F

I

A

I

L

I

V

N

A

C

W

G

G

G

F

V

T

-

S

-

P

P

S

T

G

D

H

H

K

R

A

G

S

Y

L

I

A

G

N

G

L

S

W

I

S

I

D

K

G

H

G

K

F

P

F

F

P

R

K

H

G

G

L

F

S

K

G

G

F

F

F

C

A

S

G

V

F

F

Q

T

I

T

A

A

V

A

F

F

A

S

F

F

V

S

G

G

I

T

E

E

L

V

V

I

G

G

T

L

T

A

A

A

E

E

T

V

A

D

D

N

P

P

K

Q

R

K

N

A

L

L

P

P

K

H

A

A

I

V

N

K

S

Q

I

V

P

F

V

W

R

R

I

I

I

A

I

I

F

F

Y

Y

V

V

L

V

A

S

L

L

I

I

A

L

I

I

-

G

M

L

V

L

V

I

T

S

P

P

W

D

-

D

-

P

R

N

L

L

V

M

E

G

A

N

G

G

K

S

-

T

-

S

-

V

S

S

P

P

F

F

V

V

E

L

L

A

F

I

V

K

L

E

A

A

G

N

I

I

P

K

A

G

A

L

A

P

S

S

L

V

I

F

N

N

F

A

V

V

V

I

L

I

T

I

S

S

A

V

T

V

S

S

S

V

A

T

N

N

S

S

G

S

I

T

F

Y

S

T

T

A

S

G

R

R

M

T

L

L

Y

H

G

G

L

M

A

A

E

N

E

L

Q

K

H

Q

A

A

P

P

K

S

T

F

F

F

A

K

R

Y

L

T

S

D

R

R

A

L

A

G

V

R

P

P

S

L

L

L

G

A

-

M

-

I

-

V

L

L

F

F

S

G

C

F

F

F

C

A

L

Y

L

I

L

N

G

E

A

A

A

D

M

K

I

N

-

G

Y

N

L

D

I

V

P

S

D

D

L

-

I

-

T

T

A

V

F

F

T

N

L

W

I

L

T

L

T

A

L

L

S

S

A

G

V

L

L

S

F

N

M

F

F

F

V

T

W

W

S

G

L

S

I

I

L

C

F

L

G

C

Y

H

I

I

-

I

-

F

-

R

-

L

A

A

Y

F

R

K

Q

Q

R

G

P

H

Q

S

L

L

H

K

Q

E

A

L

S

G

I

-

Y

F

K

V

M

S

P

P

G

M

G

G

V

I

F

-

M

-

C

-

W

W

-

G

A

S

C

C

L

I

A

G

F

L

F

F

V

F

F

N

V

I

L

L

V

C

L

L

L

M

S

A

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
34% identity, 72% coverage: 10:344/465 of query aligns to 83:431/590 of P04817

query
sites
P04817

H

E

L

V

R

K

R

R

S

E

L

L

S

K

N

Q

R

R

H

H

L

I

Q

G

L

M

I

I

A

A

I

L

G

G

A

T

I

I

G

G

T

T

G

G

L

L

F

F

M

I

G

G

S

L

G

S

K

T

T
x
P

I

L

S

T

L

N

A

A

G

G

P

P

-

V

S

G

I

A

L

L

L

I

V

S

Y

Y

L

L

I

F

I

M

G

G

V

S

M

L

L

A

F

Y

F

S

V

V

M

T

R

Q

A

S

M

L

G

G

E

E

L

M

L

A

L

T

S

F

N

I

L
x
P

E
x
V

Y

T

K

S

S
|
S

F

F

I

T

D

V

F

F

S

S

T

Q

D

R

L

F

L

L

G

S

P

P

W

A

A

F

G

G

F

A

C

N

G

G

W

Y

T

M

Y
|
Y

W

W

F

F

C

S

W

W

V

A

I

I

T

T

A

F

I

A

A

L

D

E

V

L

I

S

A

V

I

V

A

G

A

Q

Y

V

A

I

Q

Q

F

F

W

W

F

T

P

Y

G

K

-

V

-

P

L

L

A

A

P

A

W

W

I

I

P

S

A

I

V

F

L

W

C

V

V

I

L

I

L

T

L

I

L

M

S

-

L

-

N

N

L

L

V

F

T

P

V

V

K

K

L

Y

F

Y

G

G

E

E

L

F

E

E

F

F

W

W

F

V

A

A

L

S

I

I

K

K

I

V

V

L

A

A

I

I

V

I

A

G

L

F

I

L

A

I

T

Y

G

C

F

F

A

C

L

M

V

V

-

C

A

G

W

A

G

G

F

V

T

T

S

G

P

P

S

V

G

G

H

F

K

R

A

Y

-

W

S

R

L

N

A

P

N

G

L

A

W

W

S

G

D

P

G

G

G

I

F

I

F

S

P

K

K

D

G

K

L

N

S

E

G

G

F

R

F

F

A

L

G

G

F

W

Q

V

I

S

-

S

-

L

-

I

-

N

A

A

V

A

F

F

A

T

F

F

V

Q

G

G

I

T

E

E

L

L

V

V

G

G

T

I

T

T

A

A

A
x
G

E

E

T

A

A

A

D

N

P
|
P

K

R

R

K

N

S

L

V

P

P

K

R

A

A

I

I

N

K

S

K

I

V

P

V

V

F

R

R

I

I

I

L

I

T

F

F

Y

Y

V

I

L

G

A

S

L

L

I

L

A

F

I

I

M

G

V

L

T

V

P

P

W

Y

-

N

-

D

-

P

R

K

L

L

V

T

E

Q

A

S

-
x
T

-
x
S

-
x
Y

-

V

G

S

K

T

S

S

P

P

F

F

V

I

E

I

L

A

F

I

V

E

L

N

A

S

G

G

I

T

P

K

A

V

A

L

A

P

S

H

L

I

I

F

N

N

F

A

V

V

V

I

L

L

T

T

S

T

A

I

T

I

S

S

S

A

A

A

N

N

S

S

G

N

I

I

F

Y

S

V

T

G

S

S

R

R

M

I

L

L

Y

F

G

G

L

L

A

S

E

K

E

N

Q

K

H

L

A

A

P

P

K

K

T

F

F

L

A

S

R

R

L

T

S

T

R

K

A

G

V

V

P

P

S

Y

L

I

G

A

L

V

L

F

F

V

S

T

C

A

P113 (≠ T40) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (≠ L74) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (≠ E75) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (= S78) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (= Y99) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (≠ A228) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (= P233) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
TS 354:355 (vs. gap) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (vs. gap) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.

Sites not aligning to the query:

451 W→C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; W→L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; W→S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
461 F→S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
31% identity, 86% coverage: 11:408/465 of query aligns to 86:496/602 of P19145

query
sites
P19145

L

L

R

K

R

H

S

H

L

L

S

K

N

N

R

R

H

H

L

L

Q

Q

L

M

I

I

A

A

I

I

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

L

M

V

G

G

S

S

G

G

K

T

T

A

I

L

S

R

L

T

A

G

G

G

P

P

S

A

I

S

L

L

V

I

-

G

Y

W

L

G

I

S

I

T

G

G

V

T

M

M

L

I

F

Y

F

A

V

M

M

V

R

M

A

A

M

L

G

G

E

E

L

L

L

A

L

V

S

I

N

F

L

P

E

I

Y

S

K

G

S

G

F

F

I

T

D

T

F

Y

S

A

T

T

D

R

L

F

L

I

G

D

P

E

W

S

A

F

G

G

F

Y

F

A

C

N

G

N

W

F

T

N

Y

Y

W

M

F

L

C

Q

W

W

V

L

I

V

T

V

A

L

I

P

A

L

D

E

V

I

I

V

A

S

I

A

A

S

A

I

Y

T

A

V

Q

N

F

F

W

W

F

-

P

-

G

G

L

T

A

D

P

P

W

K

I

Y

P

R

A

D

V

G

L

F

C

V

V

A

L

L

L

F

L

W

L

L

S

A

-

I

-

V

-

I

L

I

N

N

L

M

V

F

T

G

V

V

K

K

L

G

F

Y

G

G

E

E

L

A

E

E

F

F

W

V

F

F

A

S

L

F

I

I

K

K

I

V

V

I

A

T

I

V

V

V

A

G

L

F

I

I

A

I

T

L

G

G

F

I

A

I

L

L

V

-

A

-

W

-

G

N

F

C

T

G

S

G

P

G

S

P

G

T

H

G

K

G

A

Y

S

I

L

G

A

G

N

K

L

Y

W

W

S

H

D

D

G

P

G

G

F

A

F

F

P

A

K

G

G

D

L

T

S

P

G

G

-

A

F

K

F

F

A

K

G

G

-

V

-

C

-

S

-

V

F

F

Q

V

I

T

A

A

V

A

F

F

A

S

F

F

V
x
A

G

G

I

S

E

E

L

L

V

V

G

G

T

L

T

A

A

A

A

S

E

E

T

S

A

V

D

E

P

P

K

R

R

K

N

S

L

V

P

P

K

K

A

A

I

A

N

K

S

Q

I

V

P

F

V

W

R

R

I

I

I

T

I

L

F

F

Y

Y

V

I

L

L

A

S

L

L

I

L

A

M

I

I

M

G

V

L

T

V

P

P

W

Y

R

N

L

D

-

K

-

S

-

L

-

I

-

G

-

A

-

S

V

V

E

D

A

A

G

A

K

A

S

S

P

P

F

F

V

V

E

I

L

A

F

I

V

K

L

T

A

H

G

G

I

I

P

K

A

G

A

L

A

P

S

S

L

V

I

V

N

N

F

V

V

V

V

I

L

L

T

I

S

A

A

V

T

L

S

S

S

V

A

G

N

N

S

S

G

A

I

I

F

Y

S

A

T

C

S

S

R

R

M

T

L

M

Y

V

G

A

L

L

A

A

E

E

E

Q

Q

R

H

F

A

L

P

P

K

E

T

I

F

F

A

S

R

Y

L

V

S

D

R

R

A

K

A

G

V

R

P

P

S

L

L

V

G

G

L

I

-

A

L

V

F

T

S

S

C

A

F

F

C

G

L

L

L

I

L

-

G

-

A

-

A

A

M

F

I

V

Y

A

L

A

I

S

P

K

D

K

L

E

I

G

T

E

A

V

F

F

T

N

L

W

I

L

T

L

T

A

L

L

S

S

A

G

V

L

L

S

F

S

M

L

F

F

V

T

W

W

S

G

L

G

I

I

L

C

F

I

G

C

Y

H

I

I

A

R

Y

F

R

R

R

K

-

A

-

L

-

A

Q

Q

R

G

P

R

Q

G

L

L

H

D

Q

E

A

L

S

S

I

-

Y

F

K

K

M

S

P

P

G

T

G

G

V

V

A297 (≠ V218) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

Sites not aligning to the query:

76 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
28% identity, 83% coverage: 6:391/465 of query aligns to 140:535/663 of P48813

query
sites
P48813

E

Q

H

K

P

Q

E

E

H

N

L

L

R

K

S

S

L

I

S

K

N

P

R

R

H

H

L

T

Q

V

L

M

I

M

A

S

I

L

G

G

G

T

A

G

I

I

G

G

T

T

G

G

L

L

F

L

M

V

G

G

S

N

G

S

K

K

T

V

I

L

S

N

L

N

A

A

G

G

P

P

S

G

I

G

L

L

L

I

V

I

-

G

Y

Y

L

A

I

I

I

M

G

G

V

S

M

C

L

V

F

Y

F

C

V

I

M

I

R

Q

A

A

M

C

G

G

E

E

L

L

-

A

-

V

L

I

L

Y

S

S

N

D

L

L

E

-

Y

I

K

G

S

G

F

F

I

N

D

T

F

Y

S

P

T

L

D

F

L

L

L

V

G

D

P

P

W

A

A

L

G

G

F

F

F

S

C

V

G

A

W

W

T

L

Y

F

W

C

F

L

C

Q

W

W

V

L

I

C

T

V

A

C

I

P

A

L

D

E

V

L

I

V

A

T

I

A

A

S

A

M

Y

T

A

I

Q

K

F

Y

W

W

F

T

P

T

G

S

L

V

A

N

P

P

W

D

I

V

P

F

A

V

V

V

L

I

C

F

V

Y

L

V

L

L

L

I

L

V

S

V

L

I

N

N

L

V

V

F

T

G

V

A

K

K

L

G

F

Y

G

A

E

E

L

A

E

D

F

F

W

F

F

F

A

N

L

C

I

C

K

K

I

I

V

L

A

M

I

I

V

V

A

G

L

F

I

F

A

I

T

L

G

A

F

I

A

-

L

I

V

I

A

D

W

C

G

G

F

G

T

A

S

G

P

T

S

D

G

G

H

Y

K

I

A

G

S

S

L

-

A

-

N

K

L

Y

W

W

S

R

D

D

G

P

G

G

F

A

F

F

P

R

-

G

-

D

-

T

-

P

-

I

K

Q

G

R

L

F

S

K

G

G

F

V

A

A

G

T

F

F

Q

V

I

T

A

A

V

A

F

F

A

A

F

F

V

G

G

M

I

S

E

E

L

Q

V

L

G

A

T

M

T

T

A

A

A

S

E

E

T

Q

A

S

D

N

P

P

K

R

R

K

N

A

L

I

P

P

K

S

A

A

I

A

N

K

S

K

I

M

P

I

V

Y

R

R

I

I

I

L

I

F

F

V

Y

F

V

L

L

A

A

S

L

L

I

T

A

L

I

V

M

G

V

F

V

L

T

V

P

P

W

Y

R

T

-

S

-

D

-

Q

-

L

L

L

V

G

E

A

A

A

G

G

K

S

-

A

-

T

-

K

-

A

S

S

P

P

F

Y

V

V

E

I

L

A

F

V

V

S

L

S

A

H

G

G

I

V

P

R

A

V

A

P

S

H

L

F

I

I

N

N

F

A

V

V

V

I

L

L

T

L

S

S

A

V

T

L

S

S

S

V

A

A

N

N

S

G

G

A

I

F

F

Y

S

T

T

S

S

S

R

R

M

I

L

L

Y

M

G

S

L

L

A

A

E

K

E

Q

Q

G

H

N

A

A

P

P

K

K

T

C

F

F

A

D

R

Y

L

I

S

D

R

R

A

E

A

G

V

R

P

P

S

A

L

A

G

A

L

M

L

L

F

V

S

S

C

A

-

L

F

F

C

G

L

V

L

I

L

A

G

F

A

C

A

A

M

S

I

S

Y

K

L

K

I

E

P

E

D

D

L

V

I

-

T

-

A

-

F

F

T

T

L

W

I

L

T

L

T

A

L

I

S

S

A

G

V

L

L

S

F

Q

M

L

F

F

V

T

W

W

S

I

L

T

I

I

L

C

F

L

G

S

Y

H

I

I

A

R

Y

F

R

R

Sites not aligning to the query:

132 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 82% coverage: 10:392/465 of query aligns to 276:735/852 of Q03770

query
sites
Q03770

H

H

L

I

R

Q

R

R

S

K

L

L

S

K

N

V

R

R

H

H

L

I

Q

Q

L

M

I

L

A

S

I

I

G

G

G

A

A

C

I

F

G

S

T

V

G

G

L

L

F

F

M

L

G

T

S

S

G

G

K

K

T

A

I

F

S

S

L

I

A

A

G

G

P

P

-

F

S

G

I

T

L

L

V

G

Y

F

L

G

I

L

I

T

G

G

V

S

M

I

L

I

F

L

F

A

V

T

M

M

R

L

A

S

M

F

G

T

E

E

L

L

L

S

L

T

S

L

N

I

L

P

E

V

Y

S

K

S

S

G

F

F

I

S

D

G

F

L

S

A

T

S

D

R

L

F

L

V

G

E

P

D

W

A

A

F

G

G

F

F

F

A

C

L

G

G

W

W

T

T

Y

Y

W

W

F

I

C

S

W

C

V

M

I

L

T

A

A

L

I

P

A

A

D

Q

V

V

I

S

A

S

I

S

A
x
T

A

F

Y

Y

A

L

Q

S

F

Y

W

Y

F

N

P

N

-

V

G

N

L

I

A

S

P

K

W

G

I

V

P

T

A

A

V

G

L

F

C

I

V

T

L

L

-

F

-

S

-

A

L

F

L

S

L

I

S

V

L

V

N

N

L

L

V

L

T

D

V

V

K

S

L

I

F

M

G

G

E

E

L

I

E

V

F

Y

W

V

F

A

A

G

L

I

I

S

K

K

I

V

V

I

A

I

I

A

V

I

A

L

L

M

I

V

A

F

T

T

G

M

F

I

A

I

L

L

V

N

A

A

W

G

-

H

-

G

-

N

-

D

-

I

-

H

-

E

-

G

-

V

G

G

F

F

-

R

-

Y

-

W

-

D

T

S

S

S

P

K

S

S

G

V

H

R

K

N

A

L

S

T

L

Y

A

G

-

L

-

Y

-

R

-

P

-

T

-

F

N

D

L

L

W

A

S

D

D

A

G

G

G

E

F

G

F

S

P

K

K

K

G

G

L

I

S

S

G

G

F

P

F

K

A

G

G

R

F

F

-

L

-

A

-

T

-

A

-

S

-

V

-

M

Q

L

I

I

A

S

V

T

F

F

A

A

F

F

V

S

G

G

I

V

E

E

L

M

V

T

G

F

T

L

T

A

A

S

A

G

E

E

T

A

A

I

D

N

P

P

K

R

R

K

N

T

L

I

P

P

K

S

A

A

I

T

N

K

S

R

I

T

P

F

V

S

R

I

I

V

I

L

I

I

F

S

Y

Y

V

V

L

F

A

L

L

I

I

F

A

S

I

V

M

G

V

I

V

N

-

I

-

Y

-

S

-

G

-

D

-

P

-

R

-

L

-

S

-

Y

-

F

-

P

-

G

-

I

-

S

-

E

-

K

-

R

-

Y

-

E

-

A

-

I

-

K

-

G

-

T

-

G

T

M

P

D

W

W

R

R

L

L

V

R

E

T

A

N

G

C

K

R

-

G

-

I

-

D

-

Y

-

R

-

Q

-

I

-

S

-

V

-

G

-

T

-

G

-

Y

-

S

S

S

P

P

F

W

V

V

E

V

L

A

F

L

V

Q

L

N

A

F

G

G

I

L

P

C

A

T

A

F

A

A

S

S

L

A

I

F

N

N

F

A

V

I

V

L

L

I

T

F

S

F

A

T

T

A

S

T

S

A

A

G

N

I

S

S

G

S

I

L

F

F

S

S

T

C

S

S

R

R

M

T

L

L

Y

Y

G

A

L

M

A

S

E

V

E

Q

Q

R

H

K

A

A

P

P

K

P

T

V

F

F

A

E

R

I

L

C

S

S

R

K

A

R

A

G

V

V

P

P

S

Y

L

V

G

S

L

V

L

I

F

F

S

S

C

S

F

L

C

F

L

S

L

V

L

I

G

-

A

-

A

A

M

Y

I

I

Y

A

L

V

I

D

P

Q

D

T

L

A

I

I

T

E

A

N

F

F

T

D

L

V

I

L

T

A

T

N

L

V

S

S

A

S

V

A

L

S

F

T

M

S

F

I

V

I

W

W

-

M

-

G

-

L

-

N

-

L

S

S

L

F

I

L

L

R

F

F

G

Y

Y

Y

I

A

A

L

Y

K

R

Q

R

R

Q

K

T382 (≠ A115) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

3l1lA Structure of arg-bound escherichia coli adic (see paper)
23% identity, 71% coverage: 66:394/465 of query aligns to 45:368/423 of 3l1lA

query
sites
3l1lA

M

I

G

G

E

A

L

L

L

G

L

L

S

S

N

M

L

V

E

Y

Y

A

K

K

-

M

S

S

F

F

I

L

D

D

-

P

-

S

-

P

-

G

-

S

-

Y

-

A

F

Y

S

A

T

R

D

R

L

C

L

F

G

G

P

P

W

F

A

L

G

G

F

Y

F

Q

C

T

G

N

W

V

T

L

Y

Y

W

W

F

L

-

A

C

C

W

W

V

-

I

I

T
x
G

A
x
N

I

I

A

A

D

M

V

V

I

V

A

I

I

G

A

V

A

G

Y

Y

A

L

Q

S

F

Y

W

F

F

F

P

P

G

I

L

L

A

K

-

D

P

P

W

W

I

V

P

L

A

T

V

I

L

T

C

C

V

V

L

V

L

L

-

W

-

I

-

F

L

V

S

L

L

L

N

N

L

I

V

V

T

G

V

P

K

K

L

M

F

I

G

T

E

R

L

V

E

Q

F

-

W

-

F

-

A

A

L

V

I

A

K

T

I

V

V

L

A

A

I

L

V

I

A

P

L

I

I

V

A

-

T

-

G

G

F

I

A

A

L

V

V

F

A

G

W

W

G

-

F

-

T

-

S

-

P

-

S

-

G

-

H

-

K

-

A

-

S

-

L

-

A

-

N

-

L

F

W

W

S

F

D

R

G

G

G

E

F

T

F

Y

P

-

K

-

G

-

L

M

S

A

G

A

F

I

F

Q

A

S

G

T

F

L

Q

N

I

V

A

T

V

L

F
x
W

A
x
S

F

F

V
x
I

G

G

I

V

E

E

L

S

V

A

G

S

T

V

T

A

A

A

A

G

E

V

T

V

A

K

D

N

P

P

K

K

R

R

N

N

L

V

P

P

K

I

A

A

I

T

N

I

S

G

I

G

P

V

V

L

R

I

I

A

I

V

F

C

Y

Y

V

V

L

L

A

S

L

T

I

T

A

A

I

I

M

M

V

G

V

M

T

I

P

P

W

N

R

A

L

A

V

L

E

R

A

V

G

S

K

A

S

S

P

P

F

F

V

G

E

D

L

A

F

A

V

R

L

M

A

A

G

L

I

G

P

D

A

T

A

A

A

G

S

A

L

I

I

V

N

S

F

F

V

C

V

A

L

A

T

A

S

G

A

C

T

L

S

G

S

S

A

L

N

G

S

G

G
x
W

I

T

F

L

S

L

T

A

S

G

R

Q

M

T

L

A

Y

K

G

A

L

A

A

A

E

D

Q

G

H

L

A

F

P

P

K

P

T

I

F

F

A

A

R

R

L

V

S

N

R

K

A

A

A

G

V

T

P

P

S

V

L

A

G

G

L

L

L

I

F

I

S

V

C

G

F

I

C

L

L

M

L

T

L

I

G

-

A

F

A

Q

M

L

I

S

Y

S

L

I

I

S

P

P

D

N

L

A

I

T

T

K

A

E

F

F

T

G

L

L

I

V

T

S

L

V

S

S

A

V

V

I

L

F

F

T

M

L

F

V

V

P

W

Y

-

L

-

Y

-

T

-

C

-

A

S

A

L

L

I

L

L

L

F

L

G

G

Y

H

I

G

A

H

Y

F

R

G

R

K

Q

A

R

R

P

P

binding arginine: G94 (≠ T106), N95 (≠ A107), W185 (≠ F215), S186 (≠ A216), I188 (≠ V218), W276 (≠ G306)

Sites not aligning to the query:

binding arginine: 17, 20, 21

5j4nA Crystal structure of the l-arginine/agmatine antiporter adic in complex with agmatine at 2.6 angstroem resolution (see paper)
24% identity, 71% coverage: 66:394/465 of query aligns to 47:381/437 of 5j4nA

query
sites
5j4nA

M

I

G

G

E

A

L

L

L

G

L

L

S

S

N

M

L

V

E

Y

Y

A

K

K

-

M

S

S

F

F

I

L

D

D

-

P

-

S

-

P

-

G

-

S

-

Y

-

A

F

Y

S

A

T

R

D

R

L

C

L

F

G

G

P

P

W

F

A

L

G

G

F

Y

F

Q

C

T

G

N

W

V

T

L

Y

Y

W

W

F

L

-
x
A

C
|
C

W

W

V

-

I

I

T
x
G

A
x
N

I

I

A

A

D
x
M

V

V

I

V

A

I

I

G

A

V

A

G

Y

Y

A

L

Q

S

F

Y

W

F

F

F

P

P

G

I

L

L

A

K

-

D

P

P

W

L

I

V

P

L

A

T

V

I

L

T

C

C

V

V

L

V

L

L

-

W

-

I

-

F

L

V

S

L

L

L

N

N

L

I

V

V

T

G

V

P

K

K

L

M

F

I

G

T

E

R

L

V

E

Q

F

-

W

-

F

-

A

A

L

V

I

A

K

T

I

V

V

L

A

A

I

L

V

I

A

P

L

I

I

V

A

-

T

-

G

G

F

I

A

A

L

V

V

F

A

G

W

W

G

F

F

W

T

F

S

R

P

G

S

E

G

T

H

Y

K

M

A

A

S

A

L

-

A

-

N

-

L

-

W

W

S

N

D

V

G

S

G

G

F

L

F

-

P

-

K

-

G

-

L

-

S

-

G

G

F

T

F

F

A

G

G

A

F

I

Q

Q

-

S

-

T

-

L

-

N

I

V

A

T

V

L

F

W

A

S

F

F

V

I

G

G

I

V

E

E

L

S

V

A

G

S

T

V

T

A

A

A

A

G

E

V

T

V

A

K

D

N

P

P

K

K

R

R

N

N

L

V

P

P

K

I

A

A

I

T

N

I

S

G

I

G

P

V

V

L

R

I

I

A

I

V

F

C

Y

Y

V

V

L

L

A

S

L

T

I

T

A

A

I

I

M

M

V

G

V

M

T

I

P

P

W

N

R

A

L

A

V

L

E

R

A

V

G

S

K

A

S

S

P

P

F

F

V

G

E

D

L

A

F

A

V

R

L

M

A

A

G

L

I

G

P

D

A

T

A

A

A

G

S

A

L

I

I

V

N

S

F

F

V

C

V

A

L

A

T

A

S

G

A

C

T

L

S

G

S

S

A

L

N

G

S

G

G
x
W

I

T

F

L

S

L

T

A

S

G

R

Q

M

T

L

A

Y

K

G

A

L

A

A

A

E

D

Q

G

H

L

A

F

P

P

K

P

T

I

F

F

A

A

R

R

L

V

S

N

R

K

A

A

A

G

V

T

P

P

S

V

L

A

G

G

L

L

L

I

F

I

S

V

C

G

F

I

C

L

L

M

L

T

L

I

G

-

A

F

A

Q

M

L

I

S

Y

S

L

I

I

S

P

P

D

N

L

A

I

T

T

K

A

E

F

F

T

G

L

L

I

V

T

S

L

V

S

S

A

V

V

I

L

F

F

T

M

L

F

V

V

P

W

Y

-

L

-

Y

-

T

-

C

-

A

S

A

L

L

I

L

L

L

F

L

G

G

Y

H

I

G

A

H

Y

F

R

G

R

K

Q

A

R

R

P

P

binding agmatine: A92 (vs. gap), C93 (= C102), G96 (≠ T106), N97 (≠ A107), M100 (≠ D110), W289 (≠ G306)

Sites not aligning to the query:

binding agmatine: 19

P60061 Arginine/agmatine antiporter from Escherichia coli (strain K12) (see 3 papers)
24% identity, 71% coverage: 66:394/465 of query aligns to 51:385/445 of P60061

query
sites
P60061

M

I

G

G

E

A

L

L

L

G

L

L

S

S

N

M

L

V

E

Y

Y

A

K

K

-

M

S

S

F

F

I

L

D

D

-

P

-

S

-

P

-

G

-

S

-

Y

-

A

F

Y

S

A

T

R

D

R

L

C

L

F

G

G

P

P

W

F

A

L

G

G

F

Y

F

Q

C

T

G

N

W

V

T

L

Y
|
Y

W

W

F

L

-
x
A

C
|
C

W

W

V

-

I

I

T

G

A
x
N

I

I

A

A

D
x
M

V

V

I

V

A

I

I

G

A

V

A

G

Y

Y

A

L

Q

S

F

Y

W

F

F

F

P

P

G

I

L

L

A

K

-

D

P

P

W

L

I

V

P

L

A

T

V

I

L

T

C

C

V

V

L

V

L

L

-

W

-

I

-

F

L

V

S

L

L

L

N

N

L

I

V

V

T

G

V

P

K

K

L

M

F

I

G

T

E

R

L

V

E

Q

F

-

W

-

F

-

A

A

L

V

I

A

K

T

I

V

V

L

A

A

I

L

V

I

A

P

L

I

I

V

A

-

T

-

G

G

F

I

A

A

L

V

V

F

A

G

W

W

G

F

F

W

T

F

S

R

P

G

S

E

G

T

H

Y

K

M

A

A

S

A

L

-

A

-

N

-

L

-

W

W

S

N

D

V

G

S

G

G

F

L

F

-

P

-

K

-

G

-

L

-

S

-

G

G

F

T

F

F

A

G

G

A

F

I

Q

Q

-

S

-

T