SitesBLAST
Comparing WP_015448294.1 NCBI__GCF_000230695.2:WP_015448294.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
65% identity, 100% coverage: 1:392/393 of query aligns to 1:391/392 of P33221
- M1 (= M1) modified: Initiator methionine, Removed
- EL 22:23 (= EL 22:23) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- E82 (= E82) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- R114 (= R114) binding ATP
- K155 (= K155) binding ATP
- SSGKGQ 160:165 (= SSGKGQ 160:165) binding ATP
- G162 (= G162) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
- K179 (≠ D179) modified: N6-acetyllysine
- EGVV 195:198 (≠ EGFV 195:198) binding ATP
- E203 (= E203) binding ATP
- E267 (= E267) binding Mg(2+)
- E279 (= E279) binding Mg(2+)
- D286 (= D286) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- K355 (= K356) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- RR 362:363 (= RR 363:364) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
65% identity, 99% coverage: 4:392/393 of query aligns to 3:388/389 of 1kjiA
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E264 (= E267), E276 (= E279), D283 (= D286), T284 (= T287), R360 (= R364)
- binding phosphomethylphosphonic acid adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E192 (= E195), V195 (= V198), E200 (= E203), Q222 (= Q225), E264 (= E267), F266 (= F269), E276 (= E279)
- binding magnesium ion: E264 (= E267), E276 (= E279)
1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
65% identity, 99% coverage: 4:392/393 of query aligns to 3:388/389 of 1ez1A
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E264 (= E267), E276 (= E279), D283 (= D286), T284 (= T287), R360 (= R364)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), E192 (= E195), V194 (≠ F197), V195 (= V198), F197 (= F200), E200 (= E203), Q222 (= Q225), E264 (= E267), F266 (= F269), E276 (= E279)
- binding glycinamide ribonucleotide: G20 (= G21), E21 (= E22), L22 (= L23), E81 (= E82), I82 (= I83), S160 (= S161), D283 (= D286), K352 (= K356), R359 (= R363), R360 (= R364)
- binding magnesium ion: E264 (= E267), E276 (= E279)
1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
65% identity, 99% coverage: 4:392/393 of query aligns to 3:388/389 of 1eyzA
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E264 (= E267), E276 (= E279), D283 (= D286), T284 (= T287), R360 (= R364)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E192 (= E195), V195 (= V198), F197 (= F200), E200 (= E203), E264 (= E267), F266 (= F269), E276 (= E279)
- binding magnesium ion: E264 (= E267), E276 (= E279)
1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
64% identity, 99% coverage: 4:392/393 of query aligns to 3:387/388 of 1kjqA
- active site: E114 (= E115), K154 (= K155), E263 (= E267), E275 (= E279), D282 (= D286), T283 (= T287), R359 (= R364)
- binding adenosine-5'-diphosphate: R113 (= R114), I152 (≠ V153), K154 (= K155), E191 (= E195), V193 (≠ F197), V194 (= V198), F196 (= F200), E199 (= E203), Q221 (= Q225), F265 (= F269), E275 (= E279)
- binding magnesium ion: E263 (= E267), E275 (= E279)
1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
64% identity, 99% coverage: 4:392/393 of query aligns to 3:385/386 of 1kjjA
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E261 (= E267), E273 (= E279), D280 (= D286), T281 (= T287), R357 (= R364)
- binding phosphothiophosphoric acid-adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E189 (= E195), V192 (= V198), E197 (= E203), Q219 (= Q225), E261 (= E267), F263 (= F269), E273 (= E279)
- binding magnesium ion: E261 (= E267), E273 (= E279)
1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
64% identity, 99% coverage: 4:392/393 of query aligns to 3:385/386 of 1kj8A
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E261 (= E267), E273 (= E279), D280 (= D286), T281 (= T287), R357 (= R364)
- binding adenosine-5'-triphosphate: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E189 (= E195), V192 (= V198), F194 (= F200), E197 (= E203), Q219 (= Q225), G222 (= G228), E261 (= E267), F263 (= F269), E273 (= E279)
- binding glycinamide ribonucleotide: G20 (= G21), E21 (= E22), L22 (= L23), E81 (= E82), I82 (= I83), S160 (= S161), D280 (= D286), K349 (= K356), R356 (= R363)
- binding magnesium ion: E261 (= E267), E273 (= E279)
O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
48% identity, 96% coverage: 4:382/393 of query aligns to 8:401/430 of O58056
2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
47% identity, 96% coverage: 4:382/393 of query aligns to 10:390/409 of 2dwcB
- active site: E265 (= E267), E277 (= E279), D284 (= D286), T285 (= T287), R372 (= R364)
- binding adenosine-5'-diphosphate: R120 (= R114), H159 (≠ V153), K161 (= K155), H190 (≠ F197), I191 (≠ V198), F193 (= F200), E196 (= E203), F267 (= F269), E277 (= E279)
3v4sA Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
27% identity, 94% coverage: 14:382/393 of query aligns to 20:365/380 of 3v4sA
- binding adenosine-5'-diphosphate: R106 (≠ E115), K146 (= K155), Y152 (vs. gap), G154 (= G162), Q157 (= Q165), W183 (≠ F197), V184 (= V198), E189 (= E203), N215 (≠ D229), F256 (= F269), N266 (≠ S278), E267 (= E279)
- binding carbonate ion: R271 (= R283), H273 (= H285), N274 (≠ D286)
4dlkA Crystal structure of atp-ca++ complex of purk: n5- carboxyaminoimidazole ribonucleotide synthetase (see paper)
27% identity, 94% coverage: 14:382/393 of query aligns to 21:366/380 of 4dlkA
- active site: Y153 (vs. gap), G155 (= G162), E255 (= E267), E268 (= E279), N275 (≠ D286), S276 (≠ T287), K348 (≠ R364)
- binding adenosine-5'-triphosphate: E76 (= E82), F77 (≠ I83), R107 (≠ E115), K147 (= K155), Y153 (vs. gap), D154 (≠ S161), G155 (= G162), Q158 (= Q165), W184 (≠ F197), V185 (= V198), F187 (= F200), E190 (= E203), E255 (= E267), F257 (= F269), N267 (≠ S278), E268 (= E279), R272 (= R283), H274 (= H285), N275 (≠ D286), K340 (= K356), R347 (= R363), K348 (≠ R364)
- binding calcium ion: E255 (= E267), E268 (= E279)
- binding phosphate ion: Q47 (= Q51), A49 (= A53)
3r5hA Crystal structure of adp-air complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
27% identity, 94% coverage: 14:382/393 of query aligns to 21:366/383 of 3r5hA