SitesBLAST
Comparing WP_015448846.1 NCBI__GCF_000230695.2:WP_015448846.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P76558 NADP-dependent malic enzyme; NADP-ME; EC 1.1.1.40 from Escherichia coli (strain K12) (see paper)
59% identity, 99% coverage: 3:756/763 of query aligns to 1:757/759 of P76558
- K56 (≠ S58) modified: N6-acetyllysine
6zngF Maeb full-length acetyl-coa bound state (see paper)
47% identity, 98% coverage: 11:754/763 of query aligns to 8:744/753 of 6zngF
- active site: Y38 (= Y41), A74 (= A77), K93 (= K96), E135 (= E138), D136 (= D139), D160 (= D163), D161 (= D164), N286 (= N288)
- binding acetyl coenzyme *a: R511 (≠ M518), K514 (≠ R521), Y552 (= Y560), A553 (≠ Q561), R557 (= R565), L560 (≠ R568), P571 (= P580), T590 (≠ H599), V591 (= V600), N592 (≠ Q601), L593 (≠ D602), Y625 (≠ G634), Q659 (= Q669), L690 (= L700), N694 (= N704), Q724 (≠ M734)
6zn7A Maeb malic enzyme domain apoprotein (see paper)
59% identity, 52% coverage: 11:408/763 of query aligns to 7:405/405 of 6zn7A
- active site: Y37 (= Y41), A73 (= A77), K92 (= K96), E134 (= E138), D135 (= D139), D159 (= D163), D160 (= D164), N285 (= N288)
- binding magnesium ion: E134 (= E138), D135 (= D139), D160 (= D164)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: T164 (= T168), N191 (≠ T195), A193 (= A197), G194 (= G198), A195 (= A199), S196 (≠ A200), D218 (= D222), S219 (≠ R223), K235 (= K238), L260 (= L263), S261 (= S264), V262 (≠ A265), M283 (≠ L286), N285 (= N288), V315 (= V318)
6zn4A Maeb malic enzyme domain apoprotein (see paper)
59% identity, 52% coverage: 11:408/763 of query aligns to 7:405/406 of 6zn4A
2dvmA NAD complex structure of ph1275 protein from pyrococcus horikoshii
46% identity, 52% coverage: 7:406/763 of query aligns to 1:397/438 of 2dvmA
- active site: Y37 (= Y41), R73 (≠ A77), K92 (= K96), E134 (= E138), D135 (= D139), D159 (= D163), D160 (= D164), N296 (= N288)
- binding nicotinamide-adenine-dinucleotide: T164 (= T168), G194 (= G198), A195 (= A199), A196 (= A200), V217 (≠ F221), E218 (≠ D222), L219 (≠ R223), P224 (≠ I227), F269 (≠ L263), T270 (≠ S264), L294 (= L286), N296 (= N288), N327 (= N320)
5ceeA Malic enzyme from candidatus phytoplasma aywb in complex with NAD and mg2+ (see paper)
46% identity, 48% coverage: 36:405/763 of query aligns to 29:385/387 of 5ceeA
- active site: Y34 (= Y41), A70 (= A77), K89 (= K96), E131 (= E138), D132 (= D139), D156 (= D163), D157 (= D164), N283 (= N288)
- binding magnesium ion: E131 (= E138), D132 (= D139), D157 (= D164)
- binding nicotinamide-adenine-dinucleotide: T161 (= T168), N188 (≠ T195), G189 (= G196), G191 (= G198), A193 (= A200), D213 (= D222), K214 (≠ R223), V258 (≠ L263), S259 (= S264), I263 (= I268), L281 (= L286), N283 (= N288), V312 (= V318), N314 (= N320)
2a9fA Crystal structure of a putative malic enzyme ((s)-malate:nad+ oxidoreductase (decarboxylating))
48% identity, 46% coverage: 3:354/763 of query aligns to 1:350/383 of 2a9fA
2haeD Crystal structure of a putative malic enzyme (malate oxidoreductase)
45% identity, 51% coverage: 11:400/763 of query aligns to 2:373/373 of 2haeD
2haeB Crystal structure of a putative malic enzyme (malate oxidoreductase)
45% identity, 51% coverage: 11:400/763 of query aligns to 2:373/373 of 2haeB
- active site: Y31 (= Y41), A67 (= A77), K86 (= K96), E128 (= E138), D129 (= D139), D153 (= D163), D154 (= D164), N280 (= N288)
- binding nicotinamide-adenine-dinucleotide: T158 (= T168), N185 (≠ T195), G188 (= G198), A189 (= A199), A190 (= A200), D210 (= D222), R211 (= R223), V255 (≠ L263), S256 (= S264), R257 (≠ A265), L278 (= L286), A279 (= A287), N280 (= N288), N311 (= N320)
1xcoD Crystal structure of a phosphotransacetylase from bacillus subtilis in complex with acetylphosphate (see paper)
28% identity, 42% coverage: 430:753/763 of query aligns to 6:324/325 of 1xcoD