SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_015449135.1 NCBI__GCF_000230695.2:WP_015449135.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P26276 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 10 papers)
59% identity, 59% coverage: 316:770/770 of query aligns to 12:463/463 of P26276

query
sites
P26276

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R15 (= R319) mutation to A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 25-fold.
Y17 (= Y321) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
R20 (= R324) mutation to A: No phosphoglucomutase activity.
S108 (= S412) binding via phosphate group; modified: Phosphoserine; mutation S->A,V: About 5% activity, still subject to substrate inhibition and requires G1,6P as an activator; phosphorylation occurs at a different site.; mutation to C: KM for G1P unchanged, kcat decreases 24-fold; G1,6P stimulates reaction by 2-3 orders of magnitude. No stable protein phosphorylation detected, altered ligation of metal residue.
N110 (= N414) mutation to A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 30-fold.
D242 (= D547) binding Mg(2+)
D244 (= D549) binding Mg(2+)
D246 (= D551) binding Mg(2+)
R247 (= R552) mutation to A: Small reduction in KM, small increase in dissociation of G1,6P intermediate.
R262 (= R567) mutation to A: Increases KM 2-fold, decreases kcat 9-fold for G1P. Alters flexibility of the hinge region.
K285 (= K590) binding alpha-D-glucose 1-phosphate
H308 (= H613) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
E325 (= E630) mutation to A: Reduces KM and Vmax approximately 2-fold.
EMSGH 325:329 (= EMSGH 630:634) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
H329 (= H634) mutation to A: No phosphoglucomutase activity using G1P as substrate, protein is less easily phosphorylated, no significant change in structure.
P368 (= P675) mutation to G: Increases KM 2-fold, decreases kcat 6-fold for G1P. Alters flexibility of the hinge region, structure is less compact.
R421 (= R728) mutation to C: Loss of phosphomannomutase activity, very low phosphoglucomutase activity.
RASNT 421:425 (= RASNT 728:732) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q02E40 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
59% identity, 59% coverage: 316:770/770 of query aligns to 12:463/463 of Q02E40

query
sites
Q02E40

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S108 (= S412) active site, Non-phosphorylated intermediate; modified: Phosphoserine

1pcjX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
59% identity, 59% coverage: 316:770/770 of query aligns to 7:458/458 of 1pcjX

query
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1pcjX

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K

M

M

R

K

E

E

T

T

Q

G

A

A

E

L

L

L

A

A

G

G

E
|
E

M

M

S
|
S

G

G

H
|
H

F

V

F

F

K

K

E

E

R

R

W

W

Y

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

A

S

A

A

R

R

L

L

E

E

I

I

L

L

A

S

G

Q

D

D

L

-

Q

-

G

Q

R

R

S

D

A

S

E

E

Q

H

I

V

F

F

A

S

T

A

L

F

P

P

K

S

S

D

V

I

S

S

T

T

P

P

E

E

L

I

K

N

V

I

E

T

L

V

A

T

E

E

G

D

E

S

H

K

Y

F

R

A

F

I

M

I

D

E

Q

A

L

L

R

Q

Q

R

Q

D

A

A

S

Q

F

W

E

G

D

E

A

G

S

N

L

I

I

T

T

T

I

L

D

D

G

G

V

V

R

R

A

V

D

D

W

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

D

T

P

E

V

E

A

E

L

L

K

E

R

R

I

I

Q

K

Q

T

L

V

F

F

R

R

R

N

Q

Q

L

L

L

K

A

A

V

V

N

D

P

S

G

S

L

L

K

P

L

V

P

P

F

F

active site: R15 (= R324), S103 (= S412), H104 (= H413), K113 (= K422), D237 (= D547), D239 (= D549), D241 (= D551), R242 (= R552), H324 (= H634), D335 (= D645)
binding 1-O-phosphono-alpha-D-mannopyranose: Y12 (= Y321), S103 (= S412), T301 (= T611), G302 (= G612), E320 (= E630), S322 (= S632), H324 (= H634), R416 (= R728), S418 (= S730), N419 (= N731), T420 (= T732)
binding zinc ion: S103 (= S412), D237 (= D547), D239 (= D549), D241 (= D551)

2h5aX Complex of the enzyme pmm/pgm with xylose 1-phosphate (see paper)
59% identity, 59% coverage: 316:770/770 of query aligns to 4:455/455 of 2h5aX

query
sites
2h5aX

S

S

I

I

F

F

R

R

A

A

Y
|
Y

D

D

V

I

R

R

G

G

V

V

G

G

K

D

T

T

L

L

N

T

K

A

E

E

V

T

A

A

H

Y

A

W

L

I

G

G

Q

R

S

A

I

I

G

G

T

S

L

E

M

S

V

L

E

A

K

R

G

G

L

E

R

P

E

C

I

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

A

V

A

K

A

Q

L

L

A

I

D

Q

G

G

L

L

R

V

E

D

A

C

G

G

V

C

D

Q

V

V

I

S

D

D

I

V

G

G

A

M

V

V

P

P

T

T

P

P

V

V

V

L

Y

Y

A

A

T

A

Y

N

R

V

F

L

N

E

T

G

G

K

C

S

G

G

V

V

A

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

D

D

Y

Y

N

N

G

G

F

F

K

K

I

I

V

V

G

A

G

G

Q

E

T

T

L

L

A

A

E

N

G

E

A

Q

I

I

Q

Q

D

A

L

L

Y

R

Q

E

R

R

I

I

A

E

G

K

G

N

A

D

L

L

A

A

S

S

G

G

G

V

G

G

G

S

G

-

L

V

R

E

Q

Q

V

V

D

D

V

I

A

L

P

P

D

R

Y

Y

I

F

E

K

K

Q

I

I

I

R

A

D

D

D

V

I

L

A

A

M

E

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

I

V

P

A

G

G

A

V

I

I

A

A

P

P

Q

Q

V

L

L

I

E

E

G

A

V

L

G

G

C

C

E

S

V

V

V

I

P

P

L

L

Y

Y

C

C

D

E

V

V

D

D

G

G

S

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

S

G

D

K

P

P

H

E

N

N

L

L

E

K

D

D

L

L

I

I

I

A

A

K

V

V

R

K

Q

A

T

E

G

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

G

G

V

V

T

T

R

N

S

T

G

G

E

T

I

I

V

I

Y

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

R

K

D

D

V

V

L

V

S

S

R

R

Q

N

P

P

G

G

A

A

T

D

V

I

I

I

Y

F

D

D

V

V

K

K

C

C

T

T

S

R

H

R

L

L

K

I

G

A

Q

L

I

I

L

S

D

G

A

Y

G

G

S

R

P

P

L

V

M

M

W

W

R

K

T
|
T

G
|
G

H
|
H

S

S

L

L

I

I

K

K

A

K

K

K

M

M

R

K

E

E

T

T

Q

G

A

A

E

L

L

L

A

A

G

G

E
|
E

M

M

S
|
S

G

G

H
|
H

F

V

F

F

K

K

E

E

R

R

W

W

Y

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

A

S

A

A

R

R

L

L

E

E

I

I

L

L

A

S

G

Q

D

D

L

-

Q

-

G

Q

R

R

S

D

A

S

E

E

Q

H

I

V

F

F

A

S

T

A

L

F

P

P

K

S

S

D

V

I

S

S

T

T

P

P

E

E

L

I

K

N

V

I

E

T

L

V

A

T

E

E

G

D

E

S

H

K

Y

F

R

A

F

I

M

I

D

E

Q

A

L

L

R

Q

Q

R

Q

D

A

A

S

Q

F

W

E

G

D

E

A

G

S

N

L

I

I

T

T

T

I

L

D

D

G

G

V

V

R

R

A

V

D

D

W

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

D

T

P

E

V

E

A

E

L

L

K

E

R

R

I

I

Q

K

Q

T

L

V

F

F

R

R

R

N

Q

Q

L

L

L

K

A

A

V

V

N

D

P

S

G

S

L

L

K

P

L

V

P

P

F

F

active site: H101 (= H413), D234 (= D547), D236 (= D549), D238 (= D551), R239 (= R552), D332 (= D645)
binding 1-O-phosphono-alpha-D-xylopyranose: Y9 (= Y321), T298 (= T611), G299 (= G612), H300 (= H613), E317 (= E630), S319 (= S632), H321 (= H634), R413 (= R728), S415 (= S730), N416 (= N731), T417 (= T732)
binding zinc ion: S100 (= S412), D234 (= D547), D236 (= D549), D238 (= D551)

2h4lX Complex of pmm/pgm with ribose 1-phosphate (see paper)
59% identity, 59% coverage: 316:770/770 of query aligns to 4:455/455 of 2h4lX

query
sites
2h4lX

S

S

I

I

F

F

R

R

A

A

Y
|
Y

D

D

V

I

R
|
R

G

G

V

V

G

G

K

D

T

T

L

L

N

T

K

A

E

E

V

T

A

A

H

Y

A

W

L

I

G

G

Q

R

S

A

I

I

G

G

T

S

L

E

M

S

V

L

E

A

K

R

G

G

L

E

R

P

E

C

I

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

A

V

A

K

A

Q

L

L

A

I

D

Q

G

G

L

L

R

V

E

D

A

C

G

G

V

C

D

Q

V

V

I

S

D

D

I

V

G

G

A

M

V

V

P

P

T

T

P

P

V

V

V

L

Y

Y

A

A

T

A

Y

N

R

V

F

L

N

E

T

G

G

K

C

S

G

G

V

V

A

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

D

D

Y

Y

N

N

G

G

F

F

K

K

I

I

V

V

G

A

G

G

Q

E

T

T

L

L

A

A

E

N

G

E

A

Q

I

I

Q

Q

D

A

L

L

Y

R

Q

E

R

R

I

I

A

E

G

K

G

N

A

D

L

L

A

A

S

S

G

G

G

V

G

G

G

S

G

-

L

V

R

E

Q

Q

V

V

D

D

V

I

A

L

P

P

D

R

Y

Y

I

F

E

K

K

Q

I

I

I

R

A

D

D

D

V

I

L

A

A

M

E

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

I

V

P

A

G

G

A

V

I

I

A

A

P

P

Q

Q

V

L

L

I

E

E

G

A

V

L

G

G

C

C

E

S

V

V

V

I

P

P

L

L

Y

Y

C

C

D

E

V

V

D

D

G

G

S

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

S

G

D

K

P

P

H

E

N

N

L

L

E

K

D

D

L

L

I

I

I

A

A

K

V

V

R

K

Q

A

T

E

G

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

G

G

V

V

T

T

R

N

S

T

G

G

E

T

I

I

V

I

Y

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

R

K

D

D

V

V

L

V

S

S

R

R

Q

N

P

P

G

G

A

A

T

D

V

I

I

I

Y

F

D

D

V

V

K

K

C

C

T

T

S

R

H

R

L

L

K

I

G

A

Q

L

I

I

L

S

D

G

A

Y

G

G

S

R

P

P

L

V

M

M

W

W

R

K

T
|
T

G

G

H

H

S

S

L

L

I

I

K

K

A

K

K

K

M

M

R

K

E

E

T

T

Q

G

A

A

E

L

L

L

A

A

G

G

E
|
E

M

M

S

S

G

G

H

H

F

V

F

F

K

K

E

E

R

R

W

W

Y

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

A

S

A

A

R

R

L

L

E

E

I

I

L

L

A

S

G

Q

D

D

L

-

Q

-

G

Q

R

R

S

D

A

S

E

E

Q

H

I

V

F

F

A

S

T

A

L

F

P

P

K

S

S

D

V

I

S

S

T

T

P

P

E

E

L

I

K

N

V

I

E

T

L

V

A

T

E

E

G

D

E

S

H

K

Y

F

R

A

F

I

M

I

D

E

Q

A

L

L

R

Q

Q

R

Q

D

A

A

S

Q

F

W

E

G

D

E

A

G

S

N

L

I

I

T

T

T

I

L

D

D

G

G

V

V

R

R

A

V

D

D

W

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

D

T

P

E

V

E

A

E

L

L

K

E

R

R

I

I

Q

K

Q

T

L

V

F

F

R

R

R

N

Q

Q

L

L

L

K

A

A

V

V

N

D

P

S

G

S

L

L

K

P

L

V

P

P

F

F

active site: H101 (= H413), D234 (= D547), D236 (= D549), D238 (= D551), R239 (= R552), D332 (= D645)
binding 1-O-phosphono-alpha-D-ribofuranose: Y9 (= Y321), R12 (= R324), S100 (= S412), T298 (= T611), E317 (= E630), R413 (= R728), S415 (= S730), N416 (= N731), T417 (= T732)
binding zinc ion: S100 (= S412), D234 (= D547), D236 (= D549), D238 (= D551)

2fkfA Phosphomannomutase/phosphoglucomutase from pseudomonas aeruginosa with alpha-d-glucose 1,6-bisphosphate bound (see paper)
59% identity, 59% coverage: 316:770/770 of query aligns to 4:455/455 of 2fkfA

query
sites
2fkfA

S

S

I

I

F

F

R
|
R

A

A

Y

Y

D

D

V

I

R
|
R

G

G

V

V

G

G

K

D

T

T

L

L

N

T

K

A

E

E

V

T

A

A

H

Y

A

W

L

I

G

G

Q

R

S

A

I

I

G

G

T

S

L

E

M

S

V

L

E

A

K

R

G

G

L

E

R

P

E

C

I

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

A

V

A

K

A

Q

L

L

A

I

D

Q

G

G

L

L

R

V

E

D

A

C

G

G

V

C

D

Q

V

V

I

S

D

D

I

V

G

G

A

M

V

V

P

P

T

T

P

P

V

V

V

L

Y

Y

A

A

T

A

Y

N

R

V

F

L

N

E

T

G

G

K

C

S

G

G

V

V

A

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

D

D

Y

Y

N

N

G

G

F

F

K
|
K

I

I

V

V

G

A

G

G

Q

E

T

T

L

L

A

A

E

N

G

E

A

Q

I

I

Q

Q

D

A

L

L

Y

R

Q

E

R

R

I

I

A

E

G

K

G

N

A

D

L

L

A

A

S

S

G

G

G

V

G

G

G

S

G

-

L

V

R

E

Q

Q

V

V

D

D

V

I

A

L

P

P

D

R

Y

Y

I

F

E

K

K

Q

I

I

I

R

A

D

D

D

V

I

L

A

A

M

E

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

I

V

P

A

G

G

A

V

I

I

A

A

P

P

Q

Q

V

L

L

I

E

E

G

A

V

L

G

G

C

C

E

S

V

V

V

I

P

P

L

L

Y

Y

C

C

D

E

V

V

D

D

G

G

S

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

S

G

D

K

P

P

H

E

N

N

L

L

E

K

D

D

L

L

I

I

I

A

A

K

V

V

R

K

Q

A

T

E

G

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

G

G

V

V

T

T

R

N

S

T

G

G

E

T

I

I

V

I

Y

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

R

K

D

D

V

V

L

V

S

S

R

R

Q

N

P

P

G

G

A

A

T

D

V

I

I

I

Y

F

D

D

V

V

K

K

C

C

T

T

S

R

H

R

L

L

K

I

G

A

Q

L

I

I

L

S

D

G

A

Y

G

G

S

R

P

P

L

V

M

M

W

W

R

K

T

T

G

G

H

H

S

S

L

L

I

I

K

K

A

K

K

K

M

M

R

K

E

E

T

T

Q

G

A

A

E

L

L

L

A

A

G

G

E

E

M

M

S
|
S

G

G

H
|
H

F

V

F

F

K

K

E

E

R

R

W

W

Y

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

A

S

A

A

R

R

L

L

E

E

I

I

L

L

A

S

G

Q

D

D

L

-

Q

-

G

Q

R

R

S

D

A

S

E

E

Q

H

I

V

F

F

A

S

T

A

L

F

P

P

K

S

S

D

V

I

S

S

T

T

P

P

E

E

L

I

K

N

V

I

E

T

L

V

A

T

E

E

G

D

E

S

H

K

Y

F

R

A

F

I

M

I

D

E

Q

A

L

L

R

Q

Q

R

Q

D

A

A

S

Q

F

W

E

G

D

E

A

G

S

N

L

I

I

T

T

T

I

L

D

D

G

G

V

V

R

R

A

V

D

D

W

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

D

T

P

E

V

E

A

E

L

L

K

E

R

R

I

I

Q

K

Q

T

L

V

F

F

R

R

R

N

Q

Q

L

L

L

K

A

A

V

V

N

D

P

S

G

S

L

L

K

P

L

V

P

P

F

F

active site: R12 (= R324), S100 (= S412), H101 (= H413), K110 (= K422), D234 (= D547), D236 (= D549), D238 (= D551), R239 (= R552), H321 (= H634), D332 (= D645)
binding 1,6-di-O-phosphono-alpha-D-glucopyranose: R7 (= R319), H101 (= H413), S319 (= S632), R413 (= R728), S415 (= S730), N416 (= N731), T417 (= T732)
binding zinc ion: S100 (= S412), D234 (= D547), D236 (= D549), D238 (= D551)

1pcmX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
59% identity, 59% coverage: 316:770/770 of query aligns to 4:455/455 of 1pcmX

query
sites
1pcmX

S

S

I

I

F

F

R

R

A

A

Y
|
Y

D

D

V

I

R
|
R

G

G

V

V

G

G

K

D

T

T

L

L

N

T

K

A

E

E

V

T

A

A

H

Y

A

W

L

I

G

G

Q

R

S

A

I

I

G

G

T

S

L

E

M

S

V

L

E

A

K

R

G

G

L

E

R

P

E

C

I

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

A

V

A

K

A

Q

L

L

A

I

D

Q

G

G

L

L

R

V

E

D

A

C

G

G

V

C

D

Q

V

V

I

S

D

D

I

V

G

G

A

M

V

V

P

P

T

T

P

P

V

V

V

L

Y

Y

A

A

T

A

Y

N

R

V

F

L

N

E

T

G

G

K

C

S

G

G

V

V

A

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

D

D

Y

Y

N

N

G

G

F

F

K
|
K

I

I

V

V

G

A

G

G

Q

E

T

T

L

L

A

A

E

N

G

E

A

Q

I

I

Q

Q

D

A

L

L

Y

R

Q

E

R

R

I

I

A

E

G

K

G

N

A

D

L

L

A

A

S

S

G

G

G

V

G

G

G

S

G

-

L

V

R

E

Q

Q

V

V

D

D

V

I

A

L

P

P

D

R

Y

Y

I

F

E

K

K

Q

I

I

I

R

A

D

D

D

V

I

L

A

A

M

E

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

I

V

P

A

G

G

A

V

I

I

A

A

P

P

Q

Q

V

L

L

I

E

E

G

A

V

L

G

G

C

C

E

S

V

V

V

I

P

P

L

L

Y

Y

C

C

D

E

V

V

D

D

G

G

S

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

S

G

D

K

P

P

H

E

N

N

L

L

E

K

D

D

L

L

I

I

I

A

A

K

V

V

R

K

Q

A

T

E

G

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

G

G

V

V

T

T

R

N

S

T

G

G

E

T

I

I

V

I

Y

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

R

K

D

D

V

V

L

V

S

S

R

R

Q

N

P

P

G

G

A

A

T

D

V

I

I

I

Y

F

D

D

V

V

K

K

C

C

T

T

S

R

H

R

L

L

K

I

G

A

Q

L

I

I

L

S

D

G

A

Y

G

G

S

R

P

P

L

V

M

M

W

W

R

K

T
|
T

G
|
G

H
|
H

S

S

L

L

I

I

K

K

A

K

K

K

M

M

R

K

E

E

T

T

Q

G

A

A

E

L

L

L

A

A

G

G

E
|
E

M

M

S
|
S

G

G

H
|
H

F

V

F

F

K

K

E

E

R

R

W

W

Y

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

A

S

A

A

R

R

L

L

E

E

I

I

L

L

A

S

G

Q

D

D

L

-

Q

-

G

Q

R

R

S

D

A

S

E

E

Q

H

I

V

F

F

A

S

T

A

L

F

P

P

K

S

S

D

V

I

S

S

T

T

P

P

E

E

L

I

K

N

V

I

E

T

L

V

A

T

E

E

G

D

E

S

H

K

Y

F

R

A

F

I

M

I

D

E

Q

A

L

L

R

Q

Q

R

Q

D

A

A

S

Q

F

W

E

G

D

E

A

G

S

N

L

I

I

T

T

T

I

L

D

D

G

G

V

V

R

R

A

V

D

D

W

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N

N

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

D

T

P

E

V

E

A

E

L

L

K

E

R

R

I

I

Q

K

Q

T

L

V

F

F

R

R

R

N

Q

Q

L

L

L

K

A

A

V

V

N

D

P

S

G

S

L

L

K

P

L

V

P

P

F

F

active site: R12 (= R324), S100 (= S412), H101 (= H413), K110 (= K422), D234 (= D547), D236 (= D549), D238 (= D551), R239 (= R552), H321 (= H634), D332 (= D645)
binding 6-O-phosphono-alpha-D-mannopyranose: Y9 (= Y321), S100 (= S412), T298 (= T611), G299 (= G612), H300 (= H613), E317 (= E630), S319 (= S632), H321 (= H634), R413 (= R728), S415 (= S730)
binding zinc ion: S100 (= S412), D234 (= D547), D236 (= D549), D238 (= D551)

1p5gX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
59% identity, 59% coverage: 316:770/770 of query aligns to 4:455/455 of 1p5gX

query
sites
1p5gX

S

S

I

I

F

F

R

R

A

A

Y
|
Y

D

D

V

I

R
|
R

G

G

V

V

G

G

K

D

T

T

L

L

N

T

K

A

E

E

V

T

A

A

H

Y

A

W

L

I

G

G

Q

R

S

A

I

I

G

G

T

S

L

E

M

S

V

L

E

A

K

R

G

G

L

E

R

P

E

C

I

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

A

V

A

K

A

Q

L

L

A

I

D

Q

G

G

L

L

R

V

E

D

A

C

G

G

V

C

D

Q

V

V

I

S

D

D

I

V

G

G

A

M

V

V

P

P

T

T

P

P

V

V

V

L

Y

Y

A

A

T

A

Y

N

R

V

F

L

N

E

T

G

G

K

C

S

G

G

V

V

A

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

D

D

Y

Y

N

N

G

G

F

F

K
|
K

I

I

V

V

G

A

G

G

Q

E

T

T

L

L

A

A

E

N

G

E

A

Q

I

I

Q

Q

D

A

L

L

Y

R

Q

E

R

R

I

I

A

E

G

K

G

N

A

D

L

L

A

A

S

S

G

G

G

V

G

G

G

S

G

-

L

V

R

E

Q

Q

V

V

D

D

V

I

A

L

P

P

D

R

Y

Y

I

F

E

K

K

Q

I

I

I

R

A

D

D

D

V

I

L

A

A

M

E

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

I

V

P

A

G

G

A

V

I

I

A

A

P

P

Q

Q

V

L

L

I

E

E

G

A

V

L

G

G

C

C

E

S

V

V

V

I

P

P

L

L

Y

Y

C

C

D

E

V

V

D

D

G

G

S

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

S

G

D

K

P

P

H

E

N

N

L

L

E

K

D

D

L

L

I

I

I

A

A

K

V

V

R

K

Q

A

T

E

G

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

G

G

V

V

T

T

R

N

S

T

G

G

E

T

I

I

V

I

Y

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

R

K

D

D

V

V

L

V

S

S

R

R

Q

N

P

P

G

G

A

A

T

D

V

I

I

I

Y

F

D

D

V

V

K
|
K

C

C

T

T

S

R

H

R

L

L

K

I

G

A

Q

L

I

I

L

S

D

G

A

Y

G

G

S

R

P

P

L

V

M

M

W

W

R

K

T

T

G
|
G

H
|
H

S

S

L

L

I

I

K

K

A

K

K

K

M

M

R

K

E

E

T

T

Q

G

A

A

E

L

L

L

A

A

G

G

E
|
E

M

M

S
|
S

G

G

H
|
H

F

V

F

F

K

K

E

E

R

R

W

W

Y

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

A

S

A

A

R

R

L

L

E

E

I

I

L

L

A

S

G

Q

D

D

L

-

Q

-

G

Q

R

R

S

D

A

S

E

E

Q

H

I

V

F

F

A

S

T

A

L

F

P

P

K

S

S

D

V

I

S

S

T

T

P

P

E

E

L

I

K

N

V

I

E

T

L

V

A

T

E

E

G

D

E

S

H

K

Y

F

R

A

F

I

M

I

D

E

Q

A

L

L

R

Q

Q

R

Q

D

A

A

S

Q

F

W

E

G

D

E

A

G

S

N

L

I

I

T

T

T

I

L

D

D

G

G

V

V

R

R

A

V

D

D

W

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

D

T

P

E

V

E

A

E

L

L

K

E

R

R

I

I

Q

K

Q

T

L

V

F

F

R

R

R

N

Q

Q

L

L

L

K

A

A

V

V

N

D

P

S

G

S

L

L

K

P

L

V

P

P

F

F

active site: R12 (= R324), S100 (= S412), H101 (= H413), K110 (= K422), D234 (= D547), D236 (= D549), D238 (= D551), R239 (= R552), H321 (= H634), D332 (= D645)
binding 6-O-phosphono-alpha-D-glucopyranose: Y9 (= Y321), S100 (= S412), K277 (= K590), G299 (= G612), H300 (= H613), E317 (= E630), S319 (= S632), H321 (= H634), R413 (= R728), S415 (= S730), N416 (= N731), T417 (= T732)
binding zinc ion: S100 (= S412), D234 (= D547), D236 (= D549), D238 (= D551)

1p5dX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
59% identity, 59% coverage: 316:770/770 of query aligns to 4:455/455 of 1p5dX

query
sites
1p5dX

S

S

I

I

F

F

R

R

A

A

Y
|
Y

D

D

V

I

R
|
R

G

G

V

V

G

G

K

D

T

T

L

L

N

T

K

A

E

E

V

T

A

A

H

Y

A

W

L

I

G

G

Q

R

S

A

I

I

G

G

T

S

L

E

M

S

V

L

E

A

K

R

G

G

L

E

R

P

E

C

I

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

A

V

A

K

A

Q

L

L

A

I

D

Q

G

G

L

L

R

V

E

D

A

C

G

G

V

C

D

Q

V

V

I

S

D

D

I

V

G

G

A

M

V

V

P

P

T

T

P

P

V

V

V

L

Y

Y

A

A

T

A

Y

N

R

V

F

L

N

E

T

G

G

K

C

S

G

G

V

V

A

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

D

D

Y

Y

N

N

G

G

F

F

K
|
K

I

I

V

V

G

A

G

G

Q

E

T

T

L

L

A

A

E

N

G

E

A

Q

I

I

Q

Q

D

A

L

L

Y

R

Q

E

R

R

I

I

A

E

G

K

G

N

A

D

L

L

A

A

S

S

G

G

G

V

G

G

G

S

G

-

L

V

R

E

Q

Q

V

V

D

D

V

I

A

L

P

P

D

R

Y

Y

I

F

E

K

K

Q

I

I

I

R

A

D

D

D

V

I

L

A

A

M

E

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

I

V

P

A

G

G

A

V

I

I

A

A

P

P

Q

Q

V

L

L

I

E

E

G

A

V

L

G

G

C

C

E

S

V

V

V

I

P

P

L

L

Y

Y

C

C

D

E

V

V

D

D

G

G

S

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

S

G

D

K

P

P

H

E

N

N

L

L

E

K

D

D

L

L

I

I

I

A

A

K

V

V

R

K

Q

A

T

E

G

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

G

G

V

V

T

T

R

N

S

T

G

G

E

T

I

I

V

I

Y

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

R

K

D

D

V

V

L

V

S

S

R

R

Q

N

P

P

G

G

A

A

T

D

V

I

I

I

Y

F

D

D

V

V

K

K

C

C

T

T

S

R

H

R

L

L

K

I

G

A

Q

L

I

I

L

S

D

G

A

Y

G

G

S

R

P

P

L

V

M

M

W

W

R

K

T
|
T

G
|
G

H
|
H

S

S

L

L

I

I

K

K

A

K

K

K

M

M

R

K

E

E

T

T

Q

G

A

A

E

L

L

L

A

A

G

G

E
|
E

M

M

S
|
S

G

G

H
|
H

F

V

F

F

K

K

E

E

R

R

W

W

Y

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

A

S

A

A

R

R

L

L

E

E

I

I

L

L

A

S

G

Q

D

D

L

-

Q

-

G

Q

R

R

S

D

A

S

E

E

Q

H

I

V

F

F

A

S

T

A

L

F

P

P

K

S

S

D

V

I

S

S

T

T

P

P

E

E

L

I

K

N

V

I

E

T

L

V

A

T

E

E

G

D

E

S

H

K

Y

F

R

A

F

I

M

I

D

E

Q

A

L

L

R

Q

Q

R

Q

D

A

A

S

Q

F

W

E

G

D

E

A

G

S

N

L

I

I

T

T

T

I

L

D

D

G

G

V

V

R

R

A

V

D

D

W

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N

N

T
|
T

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

D

T

P

E

V

E

A

E

L

L

K

E

R

R

I

I

Q

K

Q

T

L

V

F

F

R

R

R

N

Q

Q

L

L

L

K

A

A

V

V

N

D

P

S

G

S

L

L

K

P

L

V

P

P

F

F

active site: R12 (= R324), S100 (= S412), H101 (= H413), K110 (= K422), D234 (= D547), D236 (= D549), D238 (= D551), R239 (= R552), H321 (= H634), D332 (= D645)
binding 1-O-phosphono-alpha-D-glucopyranose: Y9 (= Y321), S100 (= S412), R239 (= R552), T298 (= T611), G299 (= G612), H300 (= H613), E317 (= E630), S319 (= S632), H321 (= H634), R413 (= R728), S415 (= S730), T417 (= T732)
binding zinc ion: S100 (= S412), D234 (= D547), D236 (= D549), D238 (= D551)

1k2yX Crystal structure of phosphomannomutase/phosphoglucomutase s108a mutant from p. Aeruginosa (see paper)
59% identity, 59% coverage: 316:770/770 of query aligns to 8:459/459 of 1k2yX

query
sites
1k2yX

S

S

I

I

F

F

R

R

A

A

Y

Y

D

D

V

I

R

R

G

G

V

V

G

G

K

D

T

T

L

L

N

T

K

A

E

E

V

T

A

A

H

Y

A

W

L

I

G

G

Q

R

S

A

I

I

G

G

T

S

L

E

M

S

V

L

E

A

K

R

G

G

L

E

R

P

E

C

I

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

A

V

A

K

A

Q

L

L

A

I

D

Q

G

G

L

L

R

V

E

D

A

C

G

G

V

C

D

Q

V

V

I

S

D

D

I

V

G

G

A

M

V

V

P

P

T

T

P

P

V

V

V

L

Y

Y

A

A

T

A

Y

N

R

V

F

L

N

E

T

G

G

K

C

S

G

G

V

V

A

M

V

L

T

T

G

G

S

A

H
|
H

N

N

P

P

D

D

Y

Y

N

N

G

G

F

F

K

K

I

I

V

V

G

A

G

G

Q

E

T

T

L

L

A

A

E

N

G

E

A

Q

I

I

Q

Q

D

A

L

L

Y

R

Q

E

R

R

I

I

A

E

G

K

G

N

A

D

L

L

A

A

S

S

G

G

G

V

G

G

G

S

G

-

L

V

R

E

Q

Q

V

V

D

D

V

I

A

L

P

P

D

R

Y

Y

I

F

E

K

K

Q

I

I

I

R

A

D

D

D

V

I

L

A

A

M

E

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

I

V

P

A

G

G

A

V

I

I

A

A

P

P

Q

Q

V

L

L

I

E

E

G

A

V

L

G

G

C

C

E

S

V

V

V

I

P

P

L

L

Y

Y

C

C

D

E

V

V

D

D

G

G

S

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

S

G

D

K

P

P

H

E

N

N

L

L

E

K

D

D

L

L

I

I

I

A

A

K

V

V

R

K

Q

A

T

E

G

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

G

G

V

V

T

T

R

N

S

T

G

G

E

T

I

I

V

I

Y

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

R

K

D

D

V

V

L

V

S

S

R

R

Q

N

P

P

G

G

A

A

T

D

V

I

I

I

Y

F

D

D

V

V

K

K

C

C

T

T

S

R

H

R

L

L

K

I

G

A

Q

L

I

I

L

S

D

G

A

Y

G

G

S

R

P

P

L

V

M

M

W

W

R

K

T

T

G

G

H

H

S

S

L

L

I

I

K

K

A

K

K

K

M

M

R

K

E

E

T

T

Q

G

A

A

E

L

L

L

A

A

G

G

E

E

M

M

S

S

G

G

H

H

F

V

F

F

K

K

E

E

R

R

W

W

Y

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

A

S

A

A

R

R

L

L

E

E

I

I

L

L

A

S

G

Q

D

D

L

-

Q

-

G

Q

R

R

S

D

A

S

E

E

Q

H

I

V

F

F

A

S

T

A

L

F

P

P

K

S

S

D

V

I

S

S

T

T

P

P

E

E

L

I

K

N

V

I

E

T

L

V

A

T

E

E

G

D

E

S

H

K

Y

F

R

A

F

I

M

I

D

E

Q

A

L

L

R

Q

Q

R

Q

D

A

A

S

Q

F

W

E

G

D

E

A

G

S

N

L

I

I

T

T

T

I

L

D

D

G

G

V

V

R

R

A

V

D

D

W

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R

R

A

A

S

S

N

N

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

D

T

P

E

V

E

A

E

L

L

K

E

R

R

I

I

Q

K

Q

T

L

V

F

F

R

R

R

N

Q

Q

L

L

L

K

A

A

V

V

N

D

P

S

G

S

L

L

K

P

L

V

P

P

F

F

active site: H105 (= H413), D238 (= D547), D240 (= D549), D242 (= D551), R243 (= R552), D336 (= D645)
binding zinc ion: D238 (= D547), D240 (= D549), D242 (= D551)

4il8A Crystal structure of an h329a mutant of p. Aeruginosa pmm/pgm (see paper)
59% identity, 59% coverage: 316:770/770 of query aligns to 8:459/459 of 4il8A

query
sites
4il8A

S

S

I

I

F

F

R

R

A

A

Y

Y

D

D

V

I

R
|
R

G

G

V

V

G

G

K

D

T

T

L

L

N

T

K

A

E

E

V

T

A

A

H

Y

A

W

L

I

G

G

Q

R

S

A

I

I

G

G

T

S

L

E

M

S

V

L

E

A

K

R

G

G

L

E

R

P

E

C

I

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

A

V

A

K

A

Q

L

L

A

I

D

Q

G

G

L

L

R

V

E

D

A

C

G

G

V

C

D

Q

V

V

I

S

D

D

I

V

G

G

A

M

V

V

P

P

T

T

P

P

V

V

V

L

Y

Y

A

A

T

A

Y

N

R

V

F

L

N

E

T

G

G

K

C

S

G

G

V

V

A

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

D

D

Y

Y

N

N

G

G

F

F

K
|
K

I

I

V

V

G

A

G

G

Q

E

T

T

L

L

A

A

E

N

G

E

A

Q

I

I

Q

Q

D

A

L

L

Y

R

Q

E

R

R

I

I

A

E

G

K

G

N

A

D

L

L

A

A

S

S

G

G

G

V

G

G

G

S

G

-

L

V

R

E

Q

Q

V

V

D

D

V

I

A

L

P

P

D

R

Y

Y

I

F

E

K

K

Q

I

I

I

R

A

D

D

D

V

I

L

A

A

M

E

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

I

V

P

A

G

G

A

V

I

I

A

A

P

P

Q

Q

V

L

L

I

E

E

G

A

V

L

G

G

C

C

E

S

V

V

V

I

P

P

L

L

Y

Y

C

C

D

E

V

V

D

D

G

G

S

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

S

G

D

K

P

P

H

E

N

N

L

L

E

K

D

D

L

L

I

I

I

A

A

K

V

V

R

K

Q

A

T

E

G

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

G

G

V

V

T

T

R

N

S

T

G

G

E

T

I

I

V

I

Y

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

R

K

D

D

V

V

L

V

S

S

R

R

Q

N

P

P

G

G

A

A

T

D

V

I

I

I

Y

F

D

D

V

V

K

K

C

C

T

T

S

R

H

R

L

L

K

I

G

A

Q

L

I

I

L

S

D

G

A

Y

G

G

S

R

P

P

L

V

M

M

W

W

R

K

T

T

G

G

H

H

S

S

L

L

I

I

K

K

A

K

K

K

M

M

R

K

E

E

T

T

Q

G

A

A

E

L

L

L

A

A

G

G

E

E

M

M

S

S

G

G

H
x
A

F

V

F

F

K

K

E

E

R

R

W

W

Y

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

A

S

A

A

R

R

L

L

E

E

I

I

L

L

A

S

G

Q

D

D

L

-

Q

-

G

Q

R

R

S

D

A

S

E

E

Q

H

I

V

F

F

A

S

T

A

L

F

P

P

K

S

S

D

V

I

S

S

T

T

P

P

E

E

L

I

K

N

V

I

E

T

L

V

A

T

E

E

G

D

E

S

H

K

Y

F

R

A

F

I

M

I

D

E

Q

A

L

L

R

Q

Q

R

Q

D

A

A

S

Q

F

W

E

G

D

E

A

G

S

N

L

I

I

T

T

T

I

L

D

D

G

G

V

V

R

R

A

V

D

D

W

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R

R

A

A

S

S

N

N

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

D

T

P

E

V

E

A

E

L

L

K

E

R

R

I

I

Q

K

Q

T

L

V

F

F

R

R

R

N

Q

Q

L

L

L

K

A

A

V

V

N

D

P

S

G

S

L

L

K

P

L

V

P

P

F

F

active site: R16 (= R324), S104 (= S412), H105 (= H413), K114 (= K422), D238 (= D547), D240 (= D549), D242 (= D551), R243 (= R552), A325 (≠ H634), D336 (= D645)
binding magnesium ion: S104 (= S412), D238 (= D547), D240 (= D549), D242 (= D551)

3rsmA Crystal structure of s108c mutant of pmm/pgm (see paper)
56% identity, 59% coverage: 316:770/770 of query aligns to 1:436/436 of 3rsmA

query
sites
3rsmA

S

S

I

I

F

F

R

R

A

A

Y

-

D

-

V

-

R

-

G

-

V

-

G

-

K

-

T

-

L

L

N

T

K

A

E

E

V

T

A

A

H

Y

A

W

L

I

G

G

Q

R

S

A

I

I

G

G

T

S

L

E

M

S

V

L

E

A

K

R

G

G

L

E

R

P

E

C

I

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

A

V

A

K

A

Q

L

L

A

I

D

Q

G

G

L

L

R

V

E

D

A

C

G

G

V

C

D

Q

V

V

I

S

D

D

I

V

G

G

A

M

V

V

P

P

T

T

P

P

V

V

V

L

Y

Y

A

A

T

A

Y

N

R

V

F

L

N

E

T

G

G

K

C

S

G

G

V

V

A

M

V

L

T

T

G

G

S
x
C

H

-

N

-

P

-

D

-

Y

-

N

N

G

G

F

F

K
|
K

I

I

V

V

G

A

G

G

Q

E

T

T

L

L

A

A

E

N

G

E

A

Q

I

I

Q

Q

D

A

L

L

Y

R

Q

E

R

R

I

I

A

E

G

K

G

N

A

D

L

L

A

A

S

S

G

G

G

V

G

G

G

S

G

-

L

V

R

E

Q

Q

V

V

D

D

V

I

A

L

P

P

D

R

Y

Y

I

F

E

K

K

Q

I

I

I

R

A

D

D

D

V

I

L

A

A

M

E

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

I

V

P

A

G

G

A

V

I

I

A

A

P

P

Q

Q

V

L

L

I

E

E

G

A

V

L

G

G

C

C

E

S

V

V

V

I

P

P

L

L

Y

Y

C

C

D

E

V

V

D

D

G

G

S

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

S

G

D

K

P

P

H

E

N

N

L

L

E

K

D

D

L

L

I

I

I

A

A

K

V

V

R

K

Q

A

T

E

G

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

G

G

V

V

T

T

R

N

S

T

G

G

E

T

I

I

V

I

Y

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

R

K

D

D

V

V

L

V

S

S

R

R

Q

N

P

P

G

G

A

A

T

D

V

I

I

I

Y

F

D

D

V

V

K

K

C

C

T

T

S

R

H

R

L

L

K

I

G

A

Q

L

I

I

L

S

D

G

A

Y

G

G

S

R

P

P

L

V

M

M

W

W

R

K

T

T

G

G

H

H

S

S

L

L

I

I

K

K

A

K

K

K

M

M

R

K

E

E

T

T

Q

G

A

A

E

L

L

L

A

A

G

G

E

E

M

M

S

S

G

G

H
|
H

F

V

F

F

K

K

E

E

R

R

W

W

Y

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

A

S

A

A

R

R

L

L

E

E

I

I

L

L

A

S

G

Q

D

D

L

-

Q

-

G

Q

R

R

S

D

A

S

E

E

Q

H

I

V

F

F

A

S

T

A

L

F

P

P

K

S

S

D

V

I

S

S

T

T

P

P

E

E

L

I

K

N

V

I

E

T

L

V

A

T

E

E

G

D

E

S

H

K

Y

F

R

A

F

I

M

I

D

E

Q

A

L

L

R

Q

Q

R

Q

D

A

A

S

Q

F

W

E

G

D

E

A

G

S

N

L

I

I

T

T

T

I

L

D

D

G

G

V

V

R

R

A

V

D

D

W

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R

R

A

A

S

S

N

N

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

D

T

P

E

V

E

A

E

L

L

K

E

R

R

I

I

Q

K

Q

T

L

V

F

F

R

R

R

N

Q

Q

L

L

L

K

A

A

V

V

N

D

P

S

G

S

L

L

K

P

L

V

P

P

F

F

active site: C87 (≠ S412), K91 (= K422), D215 (= D547), D217 (= D549), D219 (= D551), R220 (= R552), H302 (= H634), D313 (= D645)
binding phosphate ion: C87 (≠ S412), D215 (= D547), D217 (= D549), D219 (= D551), R220 (= R552)
binding zinc ion: D215 (= D547), D217 (= D549), D219 (= D551)

3uw2A X-ray crystal structure of phosphoglucomutase/phosphomannomutase family protein (bth_i1489)from burkholderia thailandensis (see paper)
55% identity, 59% coverage: 313:770/770 of query aligns to 2:458/458 of 3uw2A

query
sites
3uw2A

V

I

D

S

P

Q

S

S

I

I

F

F

R

K

A

A

Y

Y

D

D

V

I

R
|
R

G

G

V

V

V

I

G

G

K

K

T

T

L

L

N

D

K

A

E

D

V

V

A

A

H

R

A

S

L

I

G

G

Q

R

S

A

I

F

G

G

T

S

L

E

M

V

V

R

E

A

K

Q

G

G

L

G

R

D

E

A

I

V

V

V

G

A

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

A

V

A

G

A

A

L

L

A

A

D

D

G

G

L

L

R

R

E

A

A

A

G

G

V

V

D

D

V

V

I

V

D

D

I

V

G

G

A

M

V

V

P

P

T

T

P

P

V

V

V

G

Y

Y

Y

F

A

A

T

A

Y

S

-

V

-

P

-

L

-

A

-

L

-

S

-

G

-

E

R

R

F

R

N

V

T

D

G

S

C

C

G

-

V

I

A

V

V

V

T

T

G

G

S
|
S

H
|
H

N

N

P

P

D

D

Y

Y

N

N

G

G

F

F

K
|
K

I

M

V

V

V

L

G

R

G

G

Q

A

T

A

L

I

A

Y

E

G

G

D

A

Q

I

I

Q

Q

D

G

L

L

Y

Y

Q

K

R

R

I

I

A

V

G

D

G

A

A

R

L

F

A

E

S

T

G

G

G

S

G

G

G

S

G

-

L

Y

R

E

Q

Q

V

Y

D

D

V

V

A

A

P

D

D

Q

Y

Y

I

V

E

E

K

R

I

I

I

V

A

G

D

D

V

I

L

K

A

L

E

T

R

R

R

P

L

L

K

K

V

L

V

V

D

D

C

A

G

G

N

N

G

G

I

V

P

A

G

G

A

P

I

L

A

A

P

T

Q

R

V

L

L

F

E

K

G

A

V

L

G

G

C

C

E

E

V

L

V

V

P

E

L

L

Y

F

C

T

D

D

V

I

D

D

G

G

S

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

S

A

D

H

P

P

H

E

N

N

L

L

E

Q

D

D

L

V

I

I

I

A

A

K

V

L

R

K

Q

A

T

T

G

D

A

A

D

E

L

I

G

G

L

F

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

L

G

G

V

V

T

T

R

K

S

D

G

G

E

Q

I

I

V

I

Y

Y

P

P

D

D

R

R

L

Q

L

L

M

M

L

L

F

F

A

A

R

E

D

E

V

V

L

L

S

S

R

R

Q

N

P

P

G

G

A

A

T

Q

V

I

I

I

Y

Y

D

D

V

V

K

K

C

C

T

T

S

R

H

N

L

L

K

A

G

R

Q

W

I

V

L

R

D

E

A

K

G

G

S

E

P

P

L

L

M

M

W

W

R

K

T

T

G

G

H

H

S

S

L

L

I

V

K

K

A

A

K

K

M

L

R

R

E

E

T

T

Q

G

A

A

E

P

L

L

A

A

G

G

E

E

M

M

S

S

G

G

H
|
H

F

V

F

F

K

K

E

D

R

R

W

W

Y

Y

G

G

F

F

D

D

G

G

I

L

Y

Y

A

T

A

G

A

A

R

R

L

L

E

E

I

I

L

L

A

A

G

-

D

-

L

-

Q

-

G

-

R

R

S

V

A

A

E

D

-

P

-

S

Q

A

I

L

F

L

A

N

T

G

L

L

P

P

K

N

S

A

V

V

S

S

T

T

P

P

E

E

L

L

K

Q

V

L

E

N

L

V

A

-

E

-

G

-

E

-

H

-

Y

-

R

K

F

L

M

I

D

D

Q

K

L

L

R

R

Q

A

Q

D

A

A

S

K

F

F

E

D

D

G

A

A

S

D

-

E

L

V

I

V

T

T

I

I

D

D

G

G

V

L

R

R

A

V

D

E

W

Y

P

P

D

D

G

G

W

F

G

G

L

L

V

A

R

R

A

S

S

S

N

N

T

T

P

P

V

V

L

V

V

V

L

L

R

R

F

F

E

E

A

A

D

T

D

S

P

D

V

A

A

A

L

L

K

A

R

R

I

I

Q

Q

Q

D

L

D

F

F

R

R

Q

A

L

L

L

K

A

A

V

A

N

K

P

P

G

G

L

A

K

N

L

L

P

P

F

F

active site: R13 (= R324), S109 (= S412), H110 (= H413), K119 (= K422), D243 (= D547), D245 (= D549), D247 (= D551), R248 (= R552), H330 (= H634)
binding zinc ion: D243 (= D547), D245 (= D549), D247 (= D551)

1wqaA Crystal structure of pyrococcus horikoshii phosphomannomutase/phosphoglucomutase complexed with mg2+
32% identity, 55% coverage: 316:742/770 of query aligns to 3:433/455 of 1wqaA

query
sites
1wqaA

S

K

I

L

F

F

R

G

A

T

Y

F

D

G

V

V

R
|
R

G

G

V

I

V

A

G

N

K

E

T

K

L

I

N

T

K

P

E

E

V

F

A

A

H

M

A

K

L

I

G

G

Q

M

S

A

I

F

G

G

T

T

L

L

M

L

V

K

E

R

K

E

G

G

L

R

R

K

E

K

-

P

-

L

I

V

V

V

G

G

R

R

D

D

G

T

R

R

L

V

S

S

G

G

P

E

E

M

L

L

A

K

A

E

A

A

L

L

A

I

D

S

G

G

L

L

R

L

E

S

A

V

G

G

V

C

D

D

V

V

I

I

D

D

I

V

G

G

A

I

V

A

P

P

T

T

P

P

V

A

V

V

Y

Q

Y

W

A

A

T

T

Y

K

R

H

F

F

N

N

T

A

G

D

C

G

G

G

V

A

A

V

V

I

T

T

G

A

S
|
S

H
|
H

N

N

P

P

D

E

Y

Y

N

N

G

G

F

I

K
|
K

I

L

V

L

-

E

-

P

-

N

-

G

V

M

G

G

G

L

Q

K

T

K

L

E

A

R

E

E

G

A

A

I

I

V

Q

E

D

E

L

L

Y

F

Q

F

R

K

I

E

A

D

G

F

G

D

A

R

L

A

A

K

S

W

G

Y

G

E

G

I

G

G

G

E

L

V

R

R

Q

R

V

E

D

D

V

I

A

I

P

K

D

P

Y

Y

I

I

E

E

K

A

I

I

I

K

A

S

D

K

V

V

-

D

-

V

-

E

-

A

L

I

A

K

E

K

R

R

R

K

L

P

K

F

V

V

D

D

C

T

G

S

N

N

G

G

I

A

P

G

G

S

A

L

I

T

A

L

P

P

Q

Y

V

L

L

L

E

R

G

E

V

L

G

G

C

C

E

K

V

V

V

I

P

T

L

V

Y

N

C

A

D

Q

V

P

D

D

G

G

S

Y

F

F

P

P

N

A

H

R

H

N

P

P

D

E

P

P

S

N

D

E

P

-

H

E

N

N

L

L

E

K

D

E

L

F

I

M

I

E

A

I

V

V

R

K

Q

A

T

L

G

G

A

A

D

D

L

F

G

G

L

V

A

A

F

Q

D
|
D

G

G

D
|
D

G

A

D
|
D

R
|
R

L

A

G

V

V

F

V

I

T

D

R

E

S

N

G

G

E

R

I

F

V

I

Y

Q

P

G

D

D

R

K

L

T

L

F

M

A

L

L

F

V

A

A

R

D

D

A

V

V

L

L

S

K

R

E

Q

K

P

G

G

G

A

G

T

L

V

L

I

V

Y

T

D

T

V

V

K

-

C

A

T

T

S

S

H

N

L

L

K

L

G

D

Q

D

I

I

L

A

D

K

A

K

G

H

G

G

S

A

P

K

L

V

M

M

-

R

W

T

R

K

T

V

G

G

H

D

S

L

L

I

I

V

K

A

A

R

K

A

M

L

R

Y

E

E

T

N

Q

N

A

G

E

T

L

I

A

G

G

G

E

E

M

E

S

N

G

G

H
x
G

F

V

F

I

F

F

K

P

E

E

R

H

W

V

Y

L

G

G

F

-

D
x
R

D

D

G

G

I

A

Y

M

A

T

A

V

A

A

R

K

L

V

E

E

I

I

L

F

A

A

G

K

D

-

L

-

Q

S

G

G

R

K

S

K

A

F

E

S

Q

E

I

L

F

I

A

D

T

E

L

L

P

P

K

K

S

Y

V

Y

S

Q

T

I

P

K

E

T

L

K

K

R

V

-

E

-

L

H

A

V

E

E

G

G

E

D

H

R

Y

H

R

A

F

I

M

V

D

N

Q

K

L

V

R

A

Q

E

Q

M

A

A

S

R

F

E

E

R

D

G

A

Y

S

T

L

V

I

D

T

T

I

T

D

D

G

G

V

A

R

K

A

I

D

I

W

F

P

E

D

D

G

G

W

W

G

V

L

L

V

V

R

R

A

A

S

S

N

G

T

T

T

E

P

P

V

I

L

I

V

R

L

I

R

F

F

S

E

E

A

A

active site: R11 (= R324), S101 (= S412), H102 (= H413), K111 (= K422), D243 (= D547), D245 (= D549), D247 (= D551), R248 (= R552), G330 (≠ H634), R340 (≠ D645)
binding magnesium ion: S101 (= S412), D243 (= D547), D245 (= D549), D247 (= D551)

6nqhA Xanthomonas citri dephospho-pgm in complex with xylose-1-phosphate
33% identity, 57% coverage: 318:754/770 of query aligns to 6:442/448 of 6nqhA

query
sites
6nqhA

F

F

R

K

A

A

Y

Y

D

D

V

I

R
|
R

G

G

V

R

V

V

G

P

K

D

T

E

L

L

N

N

K

E

E

D

V

L

A

A

H

R

A

R

L

I

G

G

Q

V

S

A

I

L

G

A

T

A

L

-

M

Q

V

L

E

D

K

Q

G

G

L

-

R

-

E

P

I

V

V

V

V

L

G

G

R

H

D

D

G

V

R

R

L

L

S

A

G

S

P

P

E

A

L

L

A

Q

A

E

A

A

L

L

A

S

D

A

G

G

L

L

R

R

E

A

A

S

G

G

V

R

D

D

V

V

I

I

D

D

I

I

G

G

A

L

V

C

P

G

T

T

P

E

V

E

V

V

Y

Y

Y

F

A

Q

T

T

Y

D

R

Y

F

L

N

K

T

A

G

A

C

G

G

G

V

V

A

M

V

V

T

T

G

A

S
|
S

H
|
H

N

N

P

P

P

M

D

D

Y

Y

N

N

G

G

F

M

K
|
K

I

L

V

V

-

R

-

E

V

Q

G

A

G

R

Q

P

T

I

L

S

A

S

E

D

G

T

A

G

I

L

Q

F

D

A

L

I

Y

R

Q

D

R

T

I

V

A

A

G

A

G

D

A

T

L

A

A

A

S

P

G

G

G

E

G

P

G

T

G

A

L

S

R

E

Q

Q

V

S

D

R

V

T

A

D

P

K

D

T

-

A

Y

Y

I

L

E

E

K

H

I

L

A

S

-

Y

-

V

D

D

V

R

L

S

A

T

E

L

R

K

R

P

L

L

K

K

V

L

V

V

D

N

C

A

G

G

N

N

G

G

I

G

P

A

G

G

A

L

I

I

A

V

P

D

Q

L

V

L

L

A

E

P

G

H

V

L

G

P

C

F

E

E

V

F

V

V

P

R

L

V

Y

F

C

H

D

E

V

P

D

D

G

G

S

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

S

L

D

L

P

P

H

E

N

N

L

R

E

D

D

A

L

T

I

A

I

K

A

A

V

V

R

K

Q

D

T

N

G

G

A

A

D

D

L

F

G

G

L

I

A

A

F

W

D
|
D

G

G

D
|
D

G

F

D
|
D

R
|
R

L

C

G

F

V

F

T

D

R

H

S

T

G

G

E

R

I

F

V

I

Y

E

P

G

D

Y

R

Y

L

L

L

V

M

G

L

L

F

L

A

A

R

Q

D

A

V

I

L

L

S

A

R

K

Q

Q

P

P

G

G

A

G

T

K

V

V

I

V

Y

H

D

D

V

P

K
x
R

C

L

T

T

S

W

H

N

L

T

K

V

G

E

Q

Q

I

V

L

E

D

E

A

A

G

G

S

I

P

P

L

V

M

L

W

C

R

K

T
x
S

G
|
G

H

H

S

A

L

F

I

I

K

K

A

E

K

K

M

M

R

R

E

S

T

E

Q

N

A

A

E

V

L

Y

A

G

G

G

E
|
E

M

M

S
|
S

G

A

H
|
H

F

H

F

Y

F

F

K

R

E

E

R

F

W

A

Y

Y

G

A

F

-

D

D

D

S

G

G

I

M

Y

I

A

P

A

W

A

L

R

L

L

I

L

A

E

E

I

L

L

V

A

S

G

Q

D

-

L

-

Q

S

G

G

R

R

S

S

-

L

A

A

E

D

Q

L

I

V

F

E

A

A

T

R

L

M

P

Q

K

K

S

F

V

P

S

C

T

S

P

G

E

E

L

I

K

N

V

F

E

K

L

V

A

A

E

D

G

A

E

K

H

A

Y

S

R

-

F

-

M

V

D

A

Q

R

L

V

R

M

Q

E

Q

H

A

Y

S

A

F

S

E

L

D

S

A

P

S

E

L

L

I

D

T

Y

I

T

D

D

G

G

V

I

R

S

A

A

D

D

W

F

P

G

D

Q

G

W

W

R

G

F

L

N

V

L

R
|
R

A

S

S
|
S

N
|
N

T
|
T

T

E

P

P

V

L

L

L

V
x
R

L

L

R

N

F

V

E

E

A

T

D

R

D

G

P

D

V

A

A

A

L

L

-

L

-

E

K

T

R

R

I

T

Q

Q

Q

E

L

I

active site: R12 (= R324), S97 (= S412), H98 (= H413), K107 (= K422), D237 (= D547), D239 (= D549), D241 (= D551), R242 (= R552), H324 (= H634)
binding magnesium ion: D237 (= D547), D239 (= D549), D241 (= D551)
binding 1-O-phosphono-alpha-D-xylopyranose: R12 (= R324), S97 (= S412), H98 (= H413), K107 (= K422), D239 (= D549), R242 (= R552), R280 (≠ K590), S301 (≠ T611), G302 (= G612), E320 (= E630), S322 (= S632), H324 (= H634), R414 (= R728), S416 (= S730), N417 (= N731), T418 (= T732), R423 (≠ V737)

6np8A Xanthomonas citri phospho-pgm in complex with mannose-6-phosphate (see paper)
33% identity, 57% coverage: 318:754/770 of query aligns to 6:442/448 of 6np8A

query
sites
6np8A

F

F

R

K

A

A

Y
|
Y

D

D

V

I

R
|
R

G

G

V

R

V

V

G

P

K

D

T

E

L

L

N

N

K

E

E

D

V

L

A

A

H

R

A

R

L

I

G

G

Q

V

S

A

I

L

G

A

T

A

L

-

M

Q

V

L

E

D

K

Q

G

G

L

-

R

-

E

P

I

V

V

V

V

L

G

G

R

H

D

D

G

V

R

R

L

L

S

A

G

S

P

P

E

A

L

L

A

Q

A

E

A

A

L

L

A

S

D

A

G

G

L

L

R

R

E

A

A

S

G

G

V

R

D

D

V

V

I

I

D

D

I

I

G

G

A

L

V

C

P

G

T

T

P

E

V

E

V

V

Y

Y

Y

F

A

Q

T

T

Y

D

R

Y

F

L

N

K

T

A

G

A

C

G

G

G

V

V

A

M

V

V

T

T

G

A

S
|
S

H
|
H

N

N

P

P

P

M

D

D

Y

Y

N

N

G

G

F

M

K
|
K

I

L

V

V

-

R

-

E

V

Q

G

A

G

R

Q

P

T

I

L

S

A

S

E

D

G

T

A

G

I

L

Q

F

D

A

L

I

Y

R

Q

D

R

T

I

V

A

A

G

A

G

D

A

T

L

A

A

A

S

P

G

G

G

E

G

P

G

T

G

A

L

S

R

E

Q

Q

V

S

D

R

V

T

A

D

P

K

D

T

-

A

Y

Y

I

L

E

E

K

H

I

L

A

S

-

Y

-

V

D

D

V

R

L

S

A

T

E

L

R

K

R

P

L

L

K

K

V

L

V

V

D

N

C

A

G

G

N

N

G

G

I

G

P

A

G

G

A

L

I

I

A

V

P

D

Q

L

V

L

L

A

E

P

G

H

V

L

G

P

C

F

E

E

V

F

V

V

P

R

L

V

Y

F

C

H

D

E

V

P

D

D

G

G

S

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

S

L

D

L

P

P

H

E

N

N

L

R

E

D

D

A

L

T

I

A

I

K

A

A

V

V

R

K

Q

D

T

N

G

G

A

A

D

D

L

F

G

G

L

I

A

A

F

W

D
|
D

G

G

D
|
D

G

F

D
|
D

R
|
R

L

C

G

F

V

F

T

D

R

H

S

T

G

G

E

R

I

F

V

I

Y

E

P

G

D

Y

R

Y

L

L

L

V

M

G

L

L

F

L

A

A

R

Q

D

A

V

I

L

L

S

A

R

K

Q

Q

P

P

G

G

A

G

T

K

V

V

I

V

Y

H

D

D

V

P

K
x
R

C

L

T

T

S

W

H

N

L

T

K

V

G

E

Q

Q

I

V

L

E

D

E

A

A

G

G

S

I

P

P

L

V

M

L

W

C

R

K

T

S

G
|
G

H
|
H

S

A

L

F

I

I

K

K

A

E

K

K

M

M

R

R

E

S

T

E

Q

N

A

A

E

V

L

Y

A

G

G

G

E
|
E

M

M

S
|
S

G

A

H
|
H

F

H

F

Y

F

F

K

R

E

E

R

F

W

A

Y

Y

G

A

F

-

D

D

D

S

G

G

I

M

Y

I

A

P

A

W

A

L

R

L

L

I

L

A

E

E

I

L

L

V

A

S

G

Q

D

-

L

-

Q

S

G

G

R

R

S

S

-

L

A

A

E

D

Q

L

I

V

F

E

A

A

T

R

L

M

P

Q

K

K

S

F

V

P

S

C

T

S

P

G

E

E

L

I

K

N

V

F

E

K

L

V

A

A

E

D

G

A

E

K

H

A

Y

S

R

-

F

-

M

V

D

A

Q

R

L

V

R

M

Q

E

Q

H

A

Y

S

A

F

S

E

L

D

S

A

P

S

E

L

L

I

D

T

Y

I

T

D

D

G

G

V

I

R

S

A

A

D

D

W

F

P

G

D

Q

G

W

W

R

G

F

L

N

V

L

R
|
R

A

S

S
|
S

N
|
N

T
|
T

T

E

P

P

V

L

L

L

V
x
R

L

L

R

N

F

V

E

E

A

T

D

R

D

G

P

D

V

A

A

A

L

L

-

L

-

E

K

T

R

R

I

T

Q

Q

Q

E

L

I

active site: R12 (= R324), S97 (= S412), H98 (= H413), K107 (= K422), D237 (= D547), D239 (= D549), D241 (= D551), R242 (= R552), H324 (= H634)
binding calcium ion: S97 (= S412), D237 (= D547), D239 (= D549), D241 (= D551)
binding 6-O-phosphono-alpha-D-mannopyranose: Y9 (= Y321), R280 (≠ K590), G302 (= G612), H303 (= H613), E320 (= E630), S322 (= S632), H324 (= H634), R414 (= R728), S416 (= S730), N417 (= N731), T418 (= T732), R423 (≠ V737)

6nolA Xanthomonas citri dephospho-pgm in complex with mannose-1-phosphate (see paper)
33% identity, 57% coverage: 318:754/770 of query aligns to 6:442/448 of 6nolA

query
sites
6nolA

F

F

R

K

A

A

Y

Y

D

D

V

I

R
|
R

G

G

V

R

V

V

G

P

K

D

T

E

L

L

N

N

K

E

E

D

V

L

A

A

H

R

A

R

L

I

G

G

Q

V

S

A

I

L

G

A

T

A

L

-

M

Q

V

L

E

D

K

Q

G

G

L

-

R

-

E

P

I

V

V

V

V

L

G

G

R

H

D

D

G

V

R

R

L

L

S

A

G

S

P

P

E

A

L

L

A

Q

A

E

A

A

L

L

A

S

D

A

G

G

L

L

R

R

E

A

A

S

G

G

V

R

D

D

V

V

I

I

D

D

I

I

G

G

A

L

V

C

P

G

T

T

P

E

V

E

V

V

Y

Y

Y

F

A

Q

T

T

Y

D

R

Y

F

L

N

K

T

A

G

A

C

G

G

G

V

V

A

M

V

V

T

T

G

A

S
|
S

H
|
H

N

N

P

P

P

M

D

D

Y

Y

N

N

G

G

F

M

K
|
K

I

L

V

V

-

R

-

E

V

Q

G

A

G

R

Q

P

T

I

L

S

A

S

E

D

G

T

A

G

I

L

Q

F

D

A

L

I

Y

R

Q

D

R

T

I

V

A

A

G

A

G

D

A

T

L

A

A

A

S

P

G

G

G

E

G

P

G

T

G

A

L

S

R

E

Q

Q

V

S

D

R

V

T

A

D

P

K

D

T

-

A

Y

Y

I

L

E

E

K

H

I

L

A

S

-

Y

-

V

D

D

V

R

L

S

A

T

E

L

R

K

R

P

L

L

K

K

V

L

V

V

D

N

C

A

G

G

N

N

G

G

I

G

P

A

G

G

A

L

I

I

A

V

P

D

Q

L

V

L

L

A

E

P

G

H

V

L

G

P

C

F

E

E

V

F

V

V

P

R

L

V

Y

F

C

H

D

E

V

P

D

D

G

G

S

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

S

L

D

L

P

P

H

E

N

N

L

R

E

D

D

A

L

T

I

A

I

K

A

A

V

V

R

K

Q

D

T

N

G

G

A

A

D

D

L

F

G

G

L

I

A

A

F

W

D
|
D

G

G

D
|
D

G

F

D
|
D

R
|
R

L

C

G

F

V

F

T

D

R

H

S

T

G

G

E

R

I

F

V

I

Y

E

P

G

D

Y

R

Y

L

L

L

V

M

G

L

L

F

L

A

A

R

Q

D

A

V

I

L

L

S

A

R

K

Q

Q

P

P

G

G

A

G

T

K

V

V

I

V

Y

H

D

D

V

P

K

R

C

L

T

T

S

W

H

N

L

T

K

V

G

E

Q

Q

I

V

L

E

D

E

A

A

G

G

S

I

P

P

L

V

M

L

W

C

R

K

T

S

G
|
G

H

H

S

A

L

F

I

I

K

K

A

E

K

K

M

M

R

R

E

S

T

E

Q

N

A

A

E

V

L

Y

A

G

G

G

E
|
E

M

M

S
|
S

G

A

H
|
H

F

H

F

Y

F

F

K

R

E

E

R

F

W

A

Y

Y

G

A

F

-

D

D

D

S

G

G

I

M

Y

I

A

P

A

W

A

L

R

L

L

I

L

A

E

E

I

L

L

V

A

S

G

Q

D

-

L

-

Q

S

G

G

R

R

S

S

-

L

A

A

E

D

Q

L

I

V

F

E

A

A

T

R

L

M

P

Q

K

K

S

F

V

P

S

C

T

S

P

G

E

E

L

I

K

N

V

F

E

K

L

V

A

A

E

D

G

A

E

K

H

A

Y

S

R

-

F

-

M

V

D

A

Q

R

L

V

R

M

Q

E

Q

H

A

Y

S

A

F

S

E

L

D

S

A

P

S

E

L

L

I

D

T

Y

I

T

D

D

G

G

V

I

R

S

A

A

D

D

W

F

P

G

D

Q

G

W

W

R

G

F

L

N

V

L

R
|
R

A

S

S
|
S

N
|
N

T
|
T

T

E

P

P

V

L

L

L

V
x
R

L

L

R

N

F

V

E

E

A

T

D

R

D

G

P

D

V

A

A

A

L

L

-

L

-

E

K

T

R

R

I

T

Q

Q

Q

E

L

I

active site: R12 (= R324), S97 (= S412), H98 (= H413), K107 (= K422), D237 (= D547), D239 (= D549), D241 (= D551), R242 (= R552), H324 (= H634)
binding 1-O-phosphono-alpha-D-mannopyranose: G302 (= G612), E320 (= E630), S322 (= S632), H324 (= H634), R414 (= R728), S416 (= S730), N417 (= N731), T418 (= T732), R423 (≠ V737)
binding magnesium ion: S97 (= S412), D237 (= D547), D239 (= D549), D241 (= D551)

6nnpA Xanthomonas citri dephospho-pgm in complex with glucose-6-phosphate (see paper)
33% identity, 57% coverage: 318:754/770 of query aligns to 6:442/448 of 6nnpA

query
sites
6nnpA

F

F

R

K

A

A

Y

Y

D

D

V

I

R
|
R

G

G

V

R

V

V

G

P

K

D

T

E

L

L

N

N

K

E

E

D

V

L

A

A

H

R

A

R

L

I

G

G

Q

V

S

A

I

L

G

A

T

A

L

-

M

Q

V

L

E

D

K

Q

G

G

L

-

R

-

E

P

I

V

V

V

V

L

G

G

R

H

D

D

G

V

R

R

L

L

S

A

G

S

P

P

E

A

L

L

A

Q

A

E

A

A

L

L

A

S

D

A

G

G

L

L

R

R

E

A

A

S

G

G

V

R

D

D

V

V

I

I

D

D

I

I

G

G

A

L

V

C

P

G

T

T

P

E

V

E

V

V

Y

Y

Y

F

A

Q

T

T

Y

D

R

Y

F

L

N

K

T

A

G

A

C

G

G

G

V

V

A

M

V

V

T

T

G

A

S
|
S

H
|
H

N

N

P

P

P

M

D

D

Y

Y

N

N

G

G

F

M

K
|
K

I

L

V

V

-

R

-

E

V

Q

G

A

G

R

Q

P

T

I

L

S

A

S

E

D

G

T

A

G

I

L

Q

F

D

A

L

I

Y

R

Q

D

R

T

I

V

A

A

G

A

G

D

A

T

L

A

A

A

S

P

G

G

G

E

G

P

G

T

G

A

L

S

R

E

Q

Q

V

S

D

R

V

T

A

D

P

K

D

T

-

A

Y

Y

I

L

E

E

K

H

I

L

A

S

-

Y

-

V

D

D

V

R

L

S

A

T

E

L

R

K

R

P

L

L

K

K

V

L

V

V

D

N

C

A

G

G

N

N

G

G

I

G

P

A

G

G

A

L

I

I

A

V

P

D

Q

L

V

L

L

A

E

P

G

H

V

L

G

P

C

F

E

E

V

F

V

V

P

R

L

V

Y

F

C

H

D

E

V

P

D

D

G

G

S

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

S

L

D

L

P

P

H

E

N

N

L

R

E

D

D

A

L

T

I

A

I

K

A

A

V

V

R

K

Q

D

T

N

G

G

A

A

D

D

L

F

G

G

L

I

A

A

F

W

D
|
D

G

G

D
|
D

G

F

D
|
D

R
|
R

L

C

G

F

V

F

T

D

R

H

S

T

G

G

E

R

I

F

V

I

Y

E

P

G

D

Y

R

Y

L

L

L

V

M

G

L

L

F

L

A

A

R

Q

D

A

V

I

L

L

S

A

R

K

Q

Q

P

P

G

G

A

G

T

K

V

V

I

V

Y

H

D

D

V

P

K
x
R

C

L

T

T

S

W

H

N

L

T

K

V

G

E

Q

Q

I

V

L

E

D

E

A

A

G

G

S

I

P

P

L

V

M

L

W

C

R

K

T

S

G
|
G

H
|
H

S

A

L

F

I

I

K

K

A

E

K

K

M

M

R

R

E

S

T

E

Q

N

A

A

E

V

L

Y

A

G

G

G

E
|
E

M

M

S

S

G

A

H
|
H

F

H

F

Y

F

F

K

R

E

E

R

F

W

A

Y

Y

G

A

F

-

D

D

D

S

G

G

I

M

Y

I

A

P

A

W

A

L

R

L

L

I

L

A

E

E

I

L

L

V

A

S

G

Q

D

-

L

-

Q

S

G

G

R

R

S

S

-

L

A

A

E

D

Q

L

I

V

F

E

A

A

T

R

L

M

P

Q

K

K

S

F

V

P

S

C

T

S

P

G

E

E

L

I

K

N

V

F

E

K

L

V

A

A

E

D

G

A

E

K

H

A

Y

S

R

-

F

-

M

V

D

A

Q

R

L

V

R

M

Q

E

Q

H

A

Y

S

A

F

S

E

L

D

S

A

P

S

E

L

L

I

D

T

Y

I

T

D

D

G

G

V

I

R

S

A

A

D

D

W

F

P

G

D

Q

G

W

W

R

G

F

L

N

V

L

R
|
R

A

S

S
|
S

N
|
N

T
|
T

T

E

P

P

V

L

L

L

V
x
R

L

L

R

N

F

V

E

E

A

T

D

R

D

G

P

D

V

A

A

A

L

L

-

L

-

E

K

T

R

R

I

T

Q

Q

Q

E

L

I

active site: R12 (= R324), S97 (= S412), H98 (= H413), K107 (= K422), D237 (= D547), D239 (= D549), D241 (= D551), R242 (= R552), H324 (= H634)
binding 6-O-phosphono-alpha-D-glucopyranose: R280 (≠ K590), G302 (= G612), H303 (= H613), E320 (= E630), H324 (= H634), R414 (= R728), S416 (= S730), N417 (= N731), T418 (= T732), R423 (≠ V737)
binding magnesium ion: S97 (= S412), D237 (= D547), D239 (= D549), D241 (= D551)

6nn2A Xanthomonas citri pgm apo-phospho (see paper)
33% identity, 57% coverage: 318:754/770 of query aligns to 6:442/448 of 6nn2A

query
sites
6nn2A

F

F

R

K

A

A

Y

Y

D

D

V

I

R
|
R

G

G

V

R

V

V

G

P

K

D

T

E

L

L

N

N

K

E

E

D

V

L

A

A

H

R

A

R

L

I

G

G

Q

V

S

A

I

L

G

A

T

A

L

-

M

Q

V

L

E

D

K

Q

G

G

L

-

R

-

E

P

I

V

V

V

V

L

G

G

R

H

D

D

G

V

R

R

L

L

S

A

G

S

P

P

E

A

L

L

A

Q

A

E

A

A

L

L

A

S

D

A

G

G

L

L

R

R

E

A

A

S

G

G

V

R

D

D

V

V

I

I

D

D

I

I

G

G

A

L

V

C

P

G

T

T

P

E

V

E

V

V

Y

Y

Y

F

A

Q

T

T

Y

D

R

Y

F

L

N

K

T

A

G

A

C

G

G

G

V

V

A

M

V

V

T

T

G

A

S
|
S

H
|
H

N

N

P

P

P

M

D

D

Y

Y

N

N

G

G

F

M

K
|
K

I

L

V

V

-

R

-

E

V

Q

G

A

G

R

Q

P

T

I

L

S

A

S

E

D

G

T

A

G

I

L

Q

F

D

A

L

I

Y

R

Q

D

R

T

I

V

A

A

G

A

G

D

A

T

L

A

A

A

S

P

G

G

G

E

G

P

G

T

G

A

L

S

R

E

Q

Q

V

S

D

R

V

T

A

D

P

K

D

T

-

A

Y

Y

I

L

E

E

K

H

I

L

A

S

-

Y

-

V

D

D

V

R

L

S

A

T

E

L

R

K

R

P

L

L

K

K

V

L

V

V

D

N

C

A

G

G

N

N

G

G

I

G

P

A

G

G

A

L

I

I

A

V

P

D

Q

L

V

L

L

A

E

P

G

H

V

L

G

P

C

F

E

E

V

F

V

V

P

R

L

V

Y

F

C

H

D

E

V

P

D

D

G

G

S

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

S

L

D

L

P

P

H

E

N

N

L

R

E

D

D

A

L

T

I

A

I

K

A

A

V

V

R

K

Q

D

T

N

G

G

A

A

D

D

L

F

G

G

L

I

A

A

F

W

D
|
D

G

G

D
|
D

G

F

D
|
D

R
|
R

L

C

G

F

V

F

T

D

R

H

S

T

G

G

E

R

I

F

V

I

Y

E

P

G

D

Y

R

Y

L

L

L

V

M

G

L

L

F

L

A

A

R

Q

D

A

V

I

L

L

S

A

R

K

Q

Q

P

P

G

G

A

G

T

K

V

V

I

V

Y

H

D

D

V

P

K

R

C

L

T

T

S

W

H

N

L

T

K

V

G

E

Q

Q

I

V

L

E

D

E

A

A

G

G

S

I

P

P

L

V

M

L

W

C

R

K

T

S

G

G

H

H

S

A

L

F

I

I

K

K

A

E

K

K

M

M

R

R

E

S

T

E

Q

N

A

A

E

V

L

Y

A

G

G

G

E

E

M

M

S

S

G

A

H
|
H

F

H

F

Y

F

F

K

R

E

E

R

F

W

A

Y

Y

G

A

F

-

D

D

D

S

G

G

I

M

Y

I

A

P

A

W

A

L

R

L

L

I

L

A

E

E

I

L

L

V

A

S

G

Q

D

-

L

-

Q

S

G

G

R

R

S

S

-

L

A

A

E

D

Q

L

I

V

F

E

A

A

T

R

L

M

P

Q

K

K

S

F

V

P

S

C

T

S

P

G

E

E

L

I

K

N

V

F

E

K

L

V

A

A

E

D

G

A

E

K

H

A

Y

S

R

-

F

-

M

V

D

A

Q

R

L

V

R

M

Q

E

Q

H

A

Y

S

A

F

S

E

L

D

S

A

P

S

E

L

L

I

D

T

Y

I

T

D

D

G

G

V

I

R

S

A

A

D

D

W

F

P

G

D

Q

G

W

W

R

G

F

L

N

V

L

R

R

A

S

S

S

N

N

T

T

T

E

P

P

V

L

L

L

V

R

L

L

R

N

F

V

E

E

A

T

D

R

D

G

P

D

V

A

A

A

L

L

-

L

-

E

K

T

R

R

I

T

Q

Q

Q

E

L

I

active site: R12 (= R324), S97 (= S412), H98 (= H413), K107 (= K422), D237 (= D547), D239 (= D549), D241 (= D551), R242 (= R552), H324 (= H634)
binding calcium ion: S97 (= S412), D237 (= D547), D239 (= D549), D241 (= D551)

6n1eA Crystal structure of x. Citri phosphoglucomutase in complex with 1- methyl-glucose 6-phosphate (see paper)
33% identity, 57% coverage: 318:754/770 of query aligns to 6:442/448 of 6n1eA

query
sites
6n1eA

F

F

R

K

A

A

Y

Y

D

D

V

I

R

R

G

G

V

R

V

V

G

P

K

D

T

E

L

L

N

N

K

E

E

D

V

L

A

A

H

R

A

R

L

I

G

G

Q

V

S

A

I

L

G

A

T

A

L

-

M

Q

V

L

E

D

K

Q

G

G

L

-

R

-

E

P

I

V

V

V

V

L

G

G

R

H

D

D

G

V

R

R

L

L

S

A

G

S

P

P

E

A

L

L

A

Q

A

E

A

A

L

L

A

S

D

A

G

G

L

L

R

R

E

A

A

S

G

G

V

R

D

D

V

V

I

I

D

D

I

I

G

G

A

L

V

C

P

G

T

T

P

E

V

E

V

V

Y

Y

Y

F

A

Q

T

T

Y

D

R

Y

F

L

N

K

T

A

G

A

C

G

G

G

V

V

A

M

V

V

T

T

G

A

S
|
S

H

H

N

N

P

P

P

M

D

D

Y

Y

N

N

G

G

F

M

K

K

I

L

V

V

-

R

-

E

V

Q

G

A

G

R

Q

P

T

I

L

S

A

S

E

D

G

T

A

G

I

L

Q

F

D

A

L

I

Y

R

Q

D

R

T

I

V

A

A

G

A

G

D

A

T

L

A

A

A

S

P

G

G

G

E

G

P

G

T

G

A

L

S

R

E

Q

Q

V

S

D

R

V

T

A

D

P

K

D

T

-

A

Y

Y

I

L

E

E

K

H

I

L

A

S

-

Y

-

V

D

D

V

R

L

S

A

T

E

L

R

K

R

P

L

L

K

K

V

L

V

V

D

N

C

A

G

G

N

N

G

G

I

G

P

A

G

G

A

L

I

I

A

V

P

D

Q

L

V

L

L

A

E

P

G

H

V

L

G

P

C

F

E

E

V

F

V

V

P

R

L

V

Y

F

C

H

D

E

V

P

D

D

G

G

S

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

S

L

D

L

P

P

H

E

N

N

L

R

E

D

D

A

L

T

I

A

I

K

A

A

V

V

R

K

Q

D

T

N

G

G

A

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R

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binding magnesium ion: S97 (= S412), D237 (= D547), D239 (= D549), D241 (= D551)
binding 1-deoxy-7-O-phosphono-alpha-D-gluco-hept-2-ulopyranose: G302 (= G612), E320 (= E630), H324 (= H634), R414 (= R728), S416 (= S730), R423 (≠ V737)

Query Sequence

>WP_015449135.1 NCBI__GCF_000230695.2:WP_015449135.1
MALNIANERLRNLRVDWRDLLPLAGGTALLLLGLFCAWQTWLIADEGNAVRRVHLAQDEA
VRALAAEIASQRGAVEKVLASVDPATLMSDPAQSAAALRRQLPQAKKLELYSGELTEVLK
ANYREFGYAKAAQLMAAQSAEGLPLAQSVSYGNGDRRLSLVIPLGPPQRAQAWAWVELPF
APLRQRFDAISPAGGRLDLRQGDDRGYVQLLSHGTRSAEAEATGKPVAGSVFSVGAGLPG
AFIVLPRWWLLSGLLALLGLGGGGYLLRLRQRWKALPEVEEPEVPLPARQTAPARAKPPE
PPEPPAAAPVVGVDPSIFRAYDVRGVVGKTLNKEVAHALGQSIGTLMVEKGLREIVVGRD
GRLSGPELAAALADGLREAGVDVIDIGAVPTPVVYYATYRFNTGCGVAVTGSHNPPDYNG
FKIVVGGQTLAEGAIQDLYQRIAGGALASGGGGGLRQVDVAPDYIEKIIADVLAERRLKV
VVDCGNGIPGAIAPQVLEGVGCEVVPLYCDVDGSFPNHHPDPSDPHNLEDLIIAVRQTGA
DLGLAFDGDGDRLGVVTRSGEIVYPDRLLMLFARDVLSRQPGATVIYDVKCTSHLKGQIL
DAGGSPLMWRTGHSLIKAKMRETQAELAGEMSGHFFFKERWYGFDDGIYAAARLLEILAG
DLQGRSAEQIFATLPKSVSTPELKVELAEGEHYRFMDQLRQQASFEDASLITIDGVRADW
PDGWGLVRASNTTPVLVLRFEADDPVALKRIQQLFRRQLLAVNPGLKLPF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory