SitesBLAST

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
66% identity, 99% coverage: 7:444/444 of query aligns to 6:444/444 of P12425

query
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P12425

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G59 (= G60) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (= R63) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E132) binding Mg(2+)
E134 (= E134) binding Mg(2+)
E189 (= E189) binding Mg(2+)
V190 (= V190) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E196) binding Mg(2+)
G241 (= G241) binding L-glutamate
H245 (= H245) binding Mg(2+)
G302 (= G302) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (= E304) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P306) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E333) binding Mg(2+)
E424 (= E424) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
66% identity, 99% coverage: 7:444/444 of query aligns to 5:443/443 of 4lnkA

query
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4lnkA

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active site: D52 (= D54), E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), H244 (= H245), R315 (= R316), E332 (= E333), R334 (= R335)
binding adenosine-5'-diphosphate: K43 (= K45), M50 (= M52), F198 (= F199), Y200 (= Y201), N246 (= N247), S248 (= S249), S324 (≠ K325), S328 (= S329), R330 (= R331)
binding glutamic acid: E133 (= E134), E188 (= E189), V189 (= V190), N239 (= N240), G240 (= G241), G242 (= G243), E303 (= E304)
binding magnesium ion: E131 (= E132), E188 (= E189), E195 (= E196), H244 (= H245), E332 (= E333)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
66% identity, 99% coverage: 7:444/444 of query aligns to 5:443/443 of 4lniA

query
sites
4lniA

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H

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Y

Y

I

V

A

A

W

W

S

S

C

A

R

Q

N

N

R
|
R

S

S

P

P

L

L

I

I

R
|
R

I

I

P

P

A

A

K

S

R

R

G

G

P

I

S

S

T

T

R
|
R

I

V

E
|
E

L

V

R
|
R

S

S

P

V

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

S

A

V

I

L

K

A

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

N

K

K

I

L

E

E

P

A

P

P

P

A

P

P

V

I

D

D

R

R

N

N

I

I

Y

Y

E

V

L

M

S

S

A

K

E

E

R

R

K

M

A

E

L

N

G

G

I

I

D

V

C

D

L

L

P

P

G

A

S

T

L

L

I

A

E

E

A

A

L

L

Q

E

E

E

L

F

E

K

K

S

D

N

E

E

V

V

I

M

K

V

E

K

A

A

L

L

G

G

P

E

H

H

I

L

Y

F

E

E

K

H

F

F

M

I

E

E

A

A

K

K

L

E

K

I

E

E

W

W

H

D

E

M

Y

F

S

R

T

T

R

Q

V

V

H

H

P

P

W

W

E

E

I

R

E

E

T

Q

Y

Y

L

M

V

S

R

Q

Y

Y

active site: D52 (= D54), E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), H244 (= H245), R315 (= R316), E332 (= E333), R334 (= R335)
binding adenosine-5'-diphosphate: E131 (= E132), E183 (= E184), D197 (= D198), Y200 (= Y201), N246 (= N247), S248 (= S249), R320 (= R321), R330 (= R331)
binding magnesium ion: E131 (= E132), E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), E195 (= E196), H244 (= H245), E332 (= E333)
binding l-methionine-s-sulfoximine phosphate: E133 (= E134), E188 (= E189), H244 (= H245), R297 (= R298), E303 (= E304), R315 (= R316), R334 (= R335)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
65% identity, 99% coverage: 7:444/444 of query aligns to 4:439/439 of 7tdpA

query
sites
7tdpA

R

R

E

E

E

D

V

I

L

I

R

R

R

I

A

A

R

E

E

E

G

N

V

V

K

R

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

L

L

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

I

P

P

I

V

D

S

Q

Q

L

L

E

E

K

K

A

A

L

L

N

D

G

N

E

K

L

M

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

Y

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

Y

P

P

D

D

P

L

R

D

T

T

F

W

V

V

I

V

L

F

P

P

W

W

R

S

P

D

R

R

E

-

G

-

A

-

V

V

A

A

R

R

L

L

I

I

C

C

D

D

V

I

Y

Y

N

K

P

P

D

D

G

G

T

S

P

P

F

F

L

A

G

G

D

D

P

P

R

R

Y

G

A

I

L

L

K

K

R

R

V

V

L

L

E

K

E

E

A

A

A

E

E

E

L

L

G

G

Y

Y

T

T

C

S

-

M

N
|
N

A

V

G
|
G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

H

K

L

T

D

D

E

E

K

K

G

G

Q

D

P

P

T

T

I

E

T

L

H

N

D

D

Q

Q

A

G

S

G

Y

Y

F

F

D

D

L

L

S

A

P

P

I

M

D

D

L

L

G

G

E

E

E

N

A

C

R

R

E

E

I

I

I

V

L

L

T

K

L

L

E

E

K

E

M

M

G

G

F

F

E

E

I

I

E
|
E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D
|
D

F

F

K

K

Y
|
Y

S

A

D

D

A

A

L

V

D

K

V

A

A

A

D

D

K

Q

I

I

M

Q

T

T

F

F

K

K

M

L

V

V

R

K

V

T

I

I

A

A

Q

R

R

Q

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

Y

F

G

G

V

V

N

N

G
|
G

S

S

G

G

M

M

H
|
H

T

C

N
|
N

L

Q

S
|
S

L

L

S

F

R

K

N

D

G

N

E

E

N

N

A

V

F

F

Y

Y

D

D

P

E

D

T

R

D

P

E

L

L

Q

G

L

L

S

S

E

Q

V

T

A

A

Y

R

Y

H

F

Y

I

M

G

A

G

G

I

I

I

L

K

K

H

H

I

A

K

R

A

A

I

M

T

A

A

A

V

I

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

V

C

Y

Y

I

V

A

A

W

W

S

S

C

A

R

S

N

N

R
|
R

S

S

P

P

L

M

I

I

R
|
R

I

I

P

P

A

A

K

S

R

R

G

G

P

L

S

S

T

T

R
|
R

I

V

E
|
E

L

V

R
|
R

S

N

P

P

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

A

A

V

I

M

I

L

K

R

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

R

K

Q

I

M

E

A

P

L

P

P

P

A

P

P

V

I

D

D

R

R

N

N

I

I

Y

Y

E

V

L

M

S

S

A

E

E

E

R

R

K

I

A

E

L

E

G

G

I

I

D

P

C

S

L

L

P

P

G

A

S

D

L

L

I

K

E

E

A

A

L

L

Q

S

E

E

L

L

E

I

K

R

D

S

E

E

V

V

I

I

K

S

E

D

A

A

L

L

G

G

P

D

H

H

I

A

Y

L

E

A

K

Y

F

F

M

Y

E

E

A

L

K

K

L

E

K

I

E

E

W

W

H

D

E

M

Y

Y

S

R

T

T

R

Q

V

V

H

H

P

Q

W

W

E

E

I

R

E

D

T

Q

Y

Y

L

L

V

T

R

L

Y

Y

binding adenosine-5'-diphosphate: N123 (= N128), G125 (= G130), E127 (= E132), E179 (= E184), D193 (= D198), Y196 (= Y201), N242 (= N247), S244 (= S249), R316 (= R321), R326 (= R331)
binding magnesium ion: E127 (= E132), E127 (= E132), E129 (= E134), E184 (= E189), E191 (= E196), E191 (= E196), H240 (= H245), E328 (= E333)
binding l-methionine-s-sulfoximine phosphate: E127 (= E132), E129 (= E134), E184 (= E189), E191 (= E196), G236 (= G241), H240 (= H245), R293 (= R298), E299 (= E304), R311 (= R316), R330 (= R335)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
65% identity, 99% coverage: 7:444/444 of query aligns to 5:443/443 of 7tf9S

query
sites
7tf9S

R

K

E

E

E

D

V

I

L

F

R

R

R

F

A

A

R

D

E

E

E

Q

G

N

V

V

K

K

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

L

L

G

G

V

I

L

I

K

K

N

N

V

V

A

E

I

I

P

P

I

V

D

S

Q

Q

L

L

E

K

K

K

A

A

L

L

N

D

G

N

E

K

L

I

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F
|
F

V
|
V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

F

P

P

D

D

P

L

R

D

T

T

F

W

V

V

I

V

L

F

P

P

W

W

R

T

P

A

R

E

E

K

G

G

A

K

V

V

A

A

R

R

L

M

I

I

C

C

D

D

V

I

Y

Y

N

N

P

P

D

D

G

M

T

T

P

P

F

F

L

A

G

G

D

D

P

P

R

R

Y

A

A

N

L

L

K

K

R

R

V

V

L

L

E

K

E

E

A

M

A

E

E

E

L

L

G

G

Y

F

T

T

-

E

C

F

N

N

A

L

G

G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

H

K

L

L

D

D

E

E

K

N

G

R

Q

R

P

P

T

T

T

L

I

E

T

L

H

N

D

D

Q

S

A

G

S

G

Y
|
Y

F

F

D

D

L

L

S

A

P

P

I

T

D

D

L

L

G

G

E

E

E

N

A

C

R

R

E

D

I

I

I

V

L

L

T

E

L

L

E

E

K

E

M

M

G

G

F

F

E

E

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

S

E

D

D

A

A

L

I

D

T

V

A

A

C

D

D

K

S

I

I

M

Q

T

T

F

F

K

K

M

L

V

V

R

K

V

T

I

I

A

A

Q

R

R

K

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

Y

F

G

G

V

V

N

N

G
|
G

S

S

G
|
G

M

M

H
|
H

T

F

N

N

L

M

S

S

L

L

S

F

R

N

N

E

G

K

E

G

N

N

A

A

F

F

Y

F

D

D

P

E

D

S

R

G

P

E

L

L

Q

E

L

L

S

S

E

Q

V

T

A

A

Y

Y

Y

H

F

F

I

L

G

A

G

G

I

M

I

L

K

K

H

H

I

A

K

R

A

G

I

Y

T

T

A

A

V

V

T

T

N

N

P

P

T

T

V

I

N

N

S

S

Y

F

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

V

C

Y

Y

I

I

A

A

W

W

S

S

C

G

R

K

N

N

R

R

S

S

P

P

L

L

I

V

R

R

I

V

P

P

A

S

K

S

R

R

G

G

P

L

S

S

T

T

R

R

I

L

E
|
E

L

L

R

R

S

S

P

V

D

D

P

P

S

S

C

A

N

N

P

P

Y

Y

L

L

A

A

L

M

A

A

A

V

I

L

K

K

A

A

G

G

L

L

D

S

G

G

I

I

K

K

N

D

K

E

I

L

E

T

P

P

P

A

P

P

V

V

D

D

R

R

N

N

I

I

Y

Y

E

G

L

M

S

N

A

E

E

E

R

R

K

E

A

A

L

T

G

G

I

I

D

Y

C

D

L

L

P

P

G

E

S

S

L

L

I

G

E

H

A

A

L

L

Q

I

E

E

L

L

E

E

K

K

D

N

E

E

V

I

I

I

K

K

E

D

A

G

L

L

G

G

P

E

H

H

I

I

Y

F

E

E

K

H

F

F

M

I

E
|
E

A

A

K

K

L

T

K
x
I

E

E

W

C

H

D

E
x
M

Y

F

S

R

T

T

R

A

V

V

H

H

P

P

W

W

E

E

I

R

E

E

T

Q

Y

Y

L

L

V

E

R

I

Y

Y

binding glutamine: E133 (= E134), Y155 (= Y156), E188 (= E189), G240 (= G241), G242 (= G243), R297 (= R298), E303 (= E304)
binding magnesium ion: E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), H244 (= H245), E332 (= E333)
binding : F59 (= F61), V60 (= V62), E418 (= E419), I422 (≠ K423), M426 (≠ E427)

7tenA Glutamine synthetase (see paper)
65% identity, 99% coverage: 7:444/444 of query aligns to 4:442/442 of 7tenA

query
sites
7tenA

R

K

E

E

E

D

V

I

L

F

R

R

R

F

A

A

R

D

E

E

E

Q

G

N

V

V

K

K

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

L

L

G

G

V

I

L

I

K

K

N

N

V

V

A

E

I

I

P

P

I

V

D

S

Q

Q

L

L

E

K

K

K

A

A

L

L

N

D

G

N

E

K

L

I

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

F

P

P

D

D

P

L

R

D

T

T

F

W

V

V

I

V

L

F

P

P

W

W

R

T

P

A

R

E

E

K

G

G

A

K

V

V

A

A

R

R

L

M

I

I

C

C

D

D

V

I

Y

Y

N

N

P

P

D

D

G

M

T

T

P

P

F

F

L

A

G

G

D

D

P

P

R

R

Y

A

A

N

L

L

K

K

R

R

V

V

L

L

E

K

E

E

A

M

A

E

E

E

L

L

G

G

Y

F

T

T

-

E

C

F

N

N

A

L

G
|
G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

H

K

L

L

D

D

E

E

K

N

G

R

Q

R

P

P

T

T

T

L

I

E

T

L

H

N

D

D

Q

S

A

G

S

G

Y

Y

F

F

D

D

L

L

S

A

P

P

I

T

D

D

L

L

G

G

E

E

E

N

A

C

R

R

E

D

I

I

I

V

L

L

T

E

L

L

E

E

K

E

M

M

G

G

F

F

E

E

I

I

E
|
E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D
|
D

F
|
F

K
|
K

Y
|
Y

S

E

D

D

A

A

L

I

D

T

V

A

A

C

D

D

K

S

I

I

M

Q

T

T

F

F

K

K

M

L

V

V

R

K

V

T

I

I

A

A

Q

R

R

K

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

Y

F

G

G

V

V

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

T

F

N
|
N

L

M

S
|
S

L

L

S

F

R

N

N

E

G

K

E

G

N

N

A

A

F

F

Y

F

D

D

P

E

D

S

R

G

P

E

L

L

Q

E

L

L

S

S

E

Q

V

T

A

A

Y

Y

Y

H

F

F

I

L

G

A

G

G

I

M

I

L

K

K

H

H

I

A

K

R

A

G

I

Y

T

T

A

A

V

V

T

T

N

N

P

P

T

T

V

I

N

N

S

S

Y

F

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

V

C

Y

Y

I

I

A

A

W

W

S

S

C

G

R

K

N

N

R
|
R

S

S

P

P

L

L

I

V

R
|
R

I

V

P

P

A

S

K

S

R

R

G

G

P

L

S
|
S

T

T

R
|
R

I

L

E

E

L

L

R
|
R

S

S

P

V

D

D

P

P

S

S

C

A

N

N

P

P

Y

Y

L

L

A

A

L

M

A

A

A

V

I

L

K

K

A

A

G

G

L

L

D

S

G

G

I

I

K

K

N

D

K

E

I

L

E

T

P

P

P

A

P

P

V

V

D

D

R

R

N

N

I

I

Y

Y

E

G

L

M

S

N

A

E

E

E

R

R

K

E

A

A

L

T

G

G

I

I

D

Y

C

D

L

L

P

P

G

E

S

S

L

L

I

G

E

H

A

A

L

L

Q

I

E

E

L

L

E

E

K

K

D

N

E

E

V

I

I

I

K

K

E

D

A

G

L

L

G

G

P

E

H

H

I

I

Y

F

E

E

K

H

F

F

M

I

E

E

A

A

K

K

L

T

K

I

E

E

W

C

H

D

E

M

Y

F

S

R

T

T

R

A

V

V

H

H

P

P

W

W

E

E

I

R

E

E

T

Q

Y

Y

L

L

V

E

R

I

Y

Y

binding adenosine-5'-diphosphate: G128 (= G130), E130 (= E132), E182 (= E184), D196 (= D198), F197 (= F199), K198 (= K200), Y199 (= Y201), N245 (= N247), S247 (= S249), R319 (= R321), S327 (= S329), R329 (= R331)
binding l-methionine-s-sulfoximine phosphate: E130 (= E132), E132 (= E134), E187 (= E189), E194 (= E196), N238 (= N240), G239 (= G241), H243 (= H245), R296 (= R298), E302 (= E304), R314 (= R316), R333 (= R335)

7tfaB Glutamine synthetase (see paper)
65% identity, 99% coverage: 7:444/444 of query aligns to 4:441/441 of 7tfaB

query
sites
7tfaB

R

R

E

E

E

D

V

I

L

I

R

R

R

I

A

A

R

E

E

E

G

N

V

V

K

R

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

L

L

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

I

P

P

I

V

D

S

Q

Q

L

L

E

E

K

K

A

A

L

L

N

D

G

N

E

K

L

M

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F
x
Y

V

V

R
|
R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

Y

P

P

D

D

P

L

R

D

T

T

F

W

V

V

I

V

L

F

P

P

W

W

R

V

P

T

R

S

E

D

G

-

A

R

V

V

A

A

R

R

L

L

I

I

C

C

D

D

V

I

Y

Y

N

K

P

P

D

D

G

G

T

S

P

P

F

F

L

A

G

G

D

D

P

P

R

R

Y

G

A

I

L

L

K

K

R

R

V

V

L

L

E

K

E

E

A

A

A

E

E

E

L

L

G

G

Y

Y

T

T

C

S

-

M

N

N

A

V

G

G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

H

K

L

T

D

D

E

E

K

K

G

G

Q

D

P

P

T

T

I

E

T

L

H

N

D

D

Q

Q

A

G

S

G

Y
|
Y

F

F

D

D

L

L

S

A

P

P

I

M

D

D

L

L

G

G

E

E

E

N

A

C

R

R

E

E

I

I

I

V

L

L

T

K

L

L

E

E

K

E

M

M

G

G

F

F

E

E

I

I

E

E

A

A

S

S

H

H

E
|
E

V
|
V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

S

A

D

D

A

A

L

V

D

K

V

A

A

A

D

D

K

Q

I

I

M

Q

T

T

F

F

K

K

M

L

V

V

R

K

V

T

I

I

A

A

Q

R

R

Q

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

Y

F

G

G

V

V

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

T

C

N

N

L

Q

S

S

L

L

S

F

R

K

N

D

G

N

E

E

N

N

A

V

F

F

Y

Y

D

D

P

E

D

T

R

D

P

E

L

L

Q

G

L

L

S

S

E

Q

V

T

A

A

Y

R

Y

H

F

Y

I

M

G

A

G

G

I

I

I

L

K

K

H

H

I

A

K

R

A

A

I

M

T

A

A

A

V

I

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G
|
G

Y
|
Y

E
|
E

A

A

P

P

V

C

Y

Y

I

V

A

A

W

W

S

S

C

A

R

S

N

N

R
|
R

S

S

P

P

L

M

I

I

R

R

I

I

P

P

A

A

K

S

R

R

G

G

P

L

S

S

T

T

R

R

I

V

E
|
E

L

V

R

R

S

N

P

P

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

A

A

V

I

M

I

L

K

R

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

R

K

Q

I

M

E

A

P

L

P

P

P

A

P

P

V

I

D

D

R

R

N

N

I

I

Y

Y

E

V

L

M

S

S

A

E

E

E

R

R

K

I

A

E

L

E

G

G

I

I

D

P

C

S

L

L

P

P

G

A

S

D

L

L

I

K

E

E

A

A

L

L

Q

S

E

E

L

L

E

I

K

R

D

S

E

E

V

V

I

I

K

S

E

D

A

A

L

L

G

G

P

D

H

H

I

A

Y

L

E

A

K

Y

F

F

M

Y

E

E

A

L

K

K

L

E

K

I

E

E

W

W

H

D

E
x
M

Y

Y

S

R

T

T

R

Q

V

V

H

H

P

Q

W

W

E

E

I

R

E

D

T

Q

Y

Y

L

L

V

T

R

L

Y

Y

binding glutamine: E131 (= E134), Y153 (= Y156), E186 (= E189), G238 (= G241), H242 (= H245), R295 (= R298), E301 (= E304)
binding magnesium ion: E129 (= E132), E131 (= E134), E186 (= E189), E193 (= E196), H242 (= H245), E330 (= E333)
binding : Y58 (≠ F61), R60 (= R63), V187 (= V190), N237 (= N240), G299 (= G302), Y300 (= Y303), R313 (= R316), M424 (≠ E427)

7tdvC Glutamine synthetase (see paper)
63% identity, 99% coverage: 7:444/444 of query aligns to 5:443/443 of 7tdvC

query
sites
7tdvC

R

K

E

E

E

D

V

I

L

R

R

K

R

F

A

V

R

E

E

E

G

N

V

V

K

R

F

Y

I

L

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

L

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

V

P

P

I

V

D

S

Q

Q

L

L

E

E

K

K

A

V

L

L

N

D

G

N

E

E

L

M

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

H

P

P

D

D

P

L

R

D

T

T

F

W

V

V

I

I

L

F

P

P

W

W

R

T

P

A

R

G

E

Q

G

G

A

K

V

V

A

A

R

R

L

L

I

I

C

C

D

D

V

V

Y

Y

N

K

P

T

D

D

G

G

T

T

P

P

F

F

L

E

G

G

D

D

P

P

R

R

Y

A

A

N

L

L

K

K

R

R

V

V

L

L

E

K

E

E

A

M

A

E

E

D

L

L

G

G

Y

F

T

T

-

D

C

F

N

N

A

L

G
|
G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

H

K

L

L

D

D

E

E

K

K

G

G

Q

E

P

P

T

T

T

L

I

E

T

L

H

N

D

D

Q

D

A

G

S

G

Y

Y

F

F

D

D

L

L

S

A

P

P

I

T

D

D

L

L

G

G

E

E

E

N

A

C

R

R

E

D

I

I

I

V

L

L

T

E

L

L

E

E

K

D

M

M

G

G

F

F

E

D

I

I

E
|
E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D
|
D

F
|
F

K
|
K

Y
|
Y

S

A

D

D

A

A

L

V

D

T

V

A

A

C

D

D

K

N

I

I

M

Q

T

T

F

F

K

K

M

L

V

V

R

K

V

T

I

I

A

A

Q

R

R

K

H

H

G

N

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

Y

F

G

G

V

V

N

N

G
|
G

S

S

G

G

M

M

H
|
H

T

F

N
|
N

L
x
V

S
|
S

L

L

S

F

R

K

N

G

G

K

E

E

N

N

A

A

F

F

Y

F

D

D

P

P

D

N

R

T

P

E

L

M

Q

G

L

L

S

T

E

E

V

T

A

A

Y

Y

Y

Q

F

F

I

T

G

A

G

G

I

V

I

L

K

K

H

N

I

A

K

R

A

G

I

F

T

T

A

A

V

V

T

C

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

V

C

Y

Y

I

I

A

A

W

W

S

S

C

G

R

K

N

N

R
|
R

S

S

P

P

L

L

I

I

R
|
R

I

V

P

P

A

S

K

S

R

R

G

G

P

L

S
|
S

T

T

R
|
R

I

I

E
|
E

L

V

R

R

S

S

P

V

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

M

A

A

L

L

A

A

I

I

I

L

K

E

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

N

K

K

I

L

E

K

P

V

P

P

P

E

P

P

V

V

D

N

R

Q

N

N

I

I

Y

Y

E

E

L

M

S

N

A

R

E

E

R

R

K

E

A

A

L

V

G

G

I

I

D

Q

C

D

L

L

P

P

G

S

S

T

L

L

I

Y

E

T

A

A

L

L

Q

K

E

A

L

M

E

R

K

E

D

N

E

E

V

V

I

I

K

K

E

K

A

A

L

L

G

G

P

N

H

H

I

I

Y

Y

E

N

K

Q

F

F

M

I

E

N

A

S

K

K

L

S

K

I

E

E

W

W

H

D

E

Y

Y

Y

S

R

T

T

R

Q

V

V

H

S

P

E

W

W

E

E

I

R

E

D

T

Q

Y

Y

L

M

V

K

R

Q

Y

Y

binding adenosine-5'-diphosphate: G129 (= G130), E131 (= E132), E183 (= E184), D197 (= D198), F198 (= F199), K199 (= K200), Y200 (= Y201), N246 (= N247), V247 (≠ L248), S248 (= S249), R320 (= R321), S328 (= S329), R330 (= R331)
binding magnesium ion: E131 (= E132), E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), E195 (= E196), H244 (= H245), E332 (= E333)
binding l-methionine-s-sulfoximine phosphate: E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), G240 (= G241), H244 (= H245), R297 (= R298), E303 (= E304), R315 (= R316)

7tf6A Glutamine synthetase (see paper)
63% identity, 99% coverage: 7:444/444 of query aligns to 4:438/438 of 7tf6A

query
sites
7tf6A

R

K

E

E

E

D

V

I

L

R

R

K

R

F

A

A

R

E

E

E

G

N

V

V

K

R

F

Y

I

L

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

L

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

V

P

P

I

V

D

S

Q

Q

L

L

E

E

K

K

A

V

L

L

N

D

G

N

E

E

L

M

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F
|
F

V

V

R
|
R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

H

P

P

D

D

P

L

R

D

T

T

F

W

V

V

I

I

L

F

P

P

W

W

R

-

P

-

R

-

E

-

G

G

A

K

V

V

A

A

R

R

L

L

I

I

C

C

D

D

V

V

Y

Y

N

K

P

T

D

D

G

G

T

T

P

P

F

F

L

E

G

G

D

D

P

P

R

R

Y

A

A

N

L

L

K

K

R

R

V

V

L

L

E

K

E

E

A

M

A

E

E

D

L

L

G

G

Y

F

T

T

-

D

C

F

N

N

A

L

G

G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

H

K

L

L

D

D

E

E

K

K

G

G

Q

E

P

P

T

T

T

L

I

E

T

L

H

N

D

D

Q

D

A

G

S

G

Y

Y

F

F

D

D

L

L

S

A

P

P

I

T

D

D

L

L

G

G

E

E

E

N

A

C

R

R

E

D

I

I

I

V

L

L

T

E

L

L

E

E

K

D

M

M

G

G

F

F

E

D

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

S

A

D

D

A

A

L

V

D

T

V

A

A

C

D

D

K

N

I

I

M

Q

T

T

F

F

K

K

M

L

V

V

R

K

V

T

I

I

A

A

Q

R

R

K

H

H

G

N

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

Y

F

G
|
G

V

V

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

T

F

N

N

L

V

S

S

L

L

S

F

R

K

N

G

G

K

E

E

N

N

A

A

F

F

Y

F

D

D

P

P

D

N

R

T

P

E

L

M

Q

G

L

L

S

T

E

E

V

T

A

A

Y

Y

Y

Q

F

F

I

T

G

A

G

G

I

V

I

L

K

K

H

N

I

A

K

R

A

G

I

F

T

T

A

A

V

V

T

C

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G
|
G

Y
|
Y

E
|
E

A

A

P

P

V

C

Y

Y

I

I

A

A

W

W

S

S

C

G

R

K

N

N

R
|
R

S

S

P

P

L

L

I

I

R

R

I

V

P

P

A

S

K

S

R

R

G

G

P

L

S

S

T

T

R

R

I

I

E
|
E

L

V

R

R

S

S

P

V

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

M

A

A

L

L

A

A

I

I

I

L

K

E

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

N

K

K

I

L

E

K

P

V

P

P

P

E

P

P

V

V

D

N

R

Q

N

N

I

I

Y
|
Y

E

E

L

M

S

N

A

R

E

E

R

R

K

E

A

A

L

V

G

G

I

I

D

Q

C

D

L

L

P

P

G

S

S

T

L

L

I

Y

E

T

A

A

L

L

Q

K

E

A

L

M

E

R

K

E

D

N

E

E

V

V

I

I

K

K

E

K

A

A

L

L

G

G

P

N

H

H

I

I

Y

Y

E

N

K

Q

F

F

M

I

E

N

A

S

K

K

L

S

K

I

E

E

W

W

H

D

E
x
Y

Y

Y

S

R

T

T

R

Q

V

V

H

S

P

E

W

W

E

E

I

R

E

D

T
x
Q

Y

Y

L

M

V

K

R
x
Q

Y

Y

binding glutamine: E128 (= E134), E183 (= E189), G235 (= G241), H239 (= H245), R292 (= R298), E298 (= E304)
binding magnesium ion: E126 (= E132), E128 (= E134), E183 (= E189), E190 (= E196), H239 (= H245), E327 (= E333)
binding : F58 (= F61), R60 (= R63), G232 (= G238), N234 (= N240), G296 (= G302), Y297 (= Y303), R310 (= R316), Y367 (= Y373), Y421 (≠ E427), Q433 (≠ T439), Q437 (≠ R443)

8ooxB Glutamine synthetase (see paper)
63% identity, 99% coverage: 6:444/444 of query aligns to 2:438/438 of 8ooxB

query
sites
8ooxB

K

K

R

E

E

D

E

E

V

I

L

F

R

R

R

I

A

V

R

E

E

E

E

K

G

N

V

V

K

R

F

F

I

V

R

R

L

L

Q

Q

F

F

T

V

D

D

I

V

L

Q

G

G

V

I

L

P

K

K

N

N

V

V

A

A

I

I

P

P

I

V

D

G

Q

Q

L

L

E

E

K

K

A

A

L

L

N

G

G

P

E

G

L

I

M

H

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

V

L

L

R

R

P

P

D

D

P

P

R

D

T

T

F

F

V

R

I

V

L

L

P

P

W

W

R

S

P

G

R

N

E

T

G

-

A

A

V

E

A

A

R

R

L

L

I

I

C

C

D

D

V

I

Y

E

N

L

P

P

D

D

G

G

T

K

P

P

F

F

L

M

G

G

D

C

P

P

R

R

Y

Q

A

V

L

L

K

K

R

K

V

N

L

M

E

E

A

A

E

K

L

L

G

G

Y

Y

T

V

C

M

N

N

A

T

G

G

P

P

E

E

L

M

E
|
E

F

F

L

L

F

F

H

K

L

-

D

R

E

Q

K

D

G

G

Q

M

P

P

T

T

T

N

I

I

T

P

H

Q

D

D

Q

R

A

G

S

G

Y

Y

F

F

D

D

L

L

S

A

P

P

I

I

D

D

L

L

G

A

E

E

A

I

R

K

R

R

E

E

I

I

I

V

L

L

T

V

L

L

E

E

K

E

M

M

G

G

F

F

E

E

I

V

E

E

A

A

S

A

H

H

E
|
E

V

V

A

A

P

F

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

S

D

D

N

A

A

L

L

D

A

V

T

A

A

D

D

K

N

I

V

M

I

T

T

F

L

K

K

M

Y

V

V

V

A

R

K

V

T

I

L

A

A

Q

L

R

Q

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

I

Y

F

G

G

V

V

N

N

G

G

S

S

G

G

M

M

H

H

T

T

N

N

L

T

S

S

L

L

S

F

R

K

N

D

G

G

E

K

N

N

A

A

F

F

Y

Y

D

D

P

P

D

D

R

A

P

P

L

D

Q

Q

L

I

S

S

E

D

V

T

A

L

Y

R

Y

Y

F

F

I

V

G

G

I

V

I

L

K

K

H

H

I

I

K

R

A

A

I

I

T

T

A

A

V

I

T

T

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

Y

Y

E

E

A

A

P

P

V

V

Y

Y

I

I

A

T

W

W

S

S

C

G

R

P

N

N

R

R

S

S

P

S

L

L

I

I

R

R

I

V

P

P

A

A

K

P

R

R

G

G

P

N

S

S

T

T

R

R

I

I

E

E

L

I

R

R

S

S

P

P

D

D

P

P

S

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

I

I

I

L

K

A

A

A

G

G

L

L

D

D

G

G

I

V

K

K

N

N

K

K

I

I

E

E

P

P

P

E

P

R

V

V

D

E

R

K

N

N

I

I

Y

Y

E

K

L

L

S

T

A

E

E

E

R

R

K

E

A

K

L

L

G

G

I

I

D

G

C

M

L

L

P

P

G

G

S

T

L

L

I

K

E

E

A

A

L

I

Q

E

E

C

L

F

E

K

K

E

D

D

E

E

V

L

I

L

K

V

E

S

A

A

L

L

G

G

P

E

H

H

I

V

Y

S

E

Q

K

S

F

I

M

I

E

N

A

V

K

A

L

M

K

A

E

D

W

W

H

D

E

S

Y

Y

S

R

T

T

R

Q

V

V

H

H

P

Q

W

W

E

E

I

L

E

D

T

R

Y

Y

L

L

V

Q

R

T

Y

Y

binding magnesium ion: E129 (= E134), E183 (= E189), E190 (= E196)

8ufjB Glutamine synthetase (see paper)
61% identity, 99% coverage: 1:441/444 of query aligns to 1:441/444 of 8ufjB

query
sites
8ufjB

M

M

E

K

L

C

M

T

K

T

R

K

E

E

E

D

V

V

L

L

R

E

R

A

A

V

R

K

E

E

E

R

G

D

V

V

K

K

F

F

I

I

R

R

L

T

Q

Q

F

F

T

T

D

D

I

T

L

L

G

G

V

I

L

I

K

K

N

S

V

W

A

A

I

I

P

P

I

A

D

E

Q

Q

L

L

E

E

K

E

A

A

L

F

N

E

G

N

E

G

L

V

M

M

F

F

D

D

G

G

S

S

I

I

E

Q

G

G

F

F

V

T

R

R

I

I

E

E

S

S

D

D

M

M

Y

K

L

L

R

A

P

L

D

D

P

P

R

S

T

T

F

F

V

R

I

I

L

L

P

P

W

W

R

R

P

P

R

A

E

T

G

G

A

A

V

V

A

A

R

R

L

I

I

L

C

G

D

D

V

V

Y

Y

N

L

P

P

D

D

G

G

T

N

P

P

F

F

L

K

G

G

D

D

P

P

R

R

Y

Y

A

V

L

L

K

K

R

T

V

A

L

I

E

K

E

E

A

A

A

E

E

K

L

M

G

G

Y

F

T

S

C

M

N

N

A

V

G

G

P

P

E
|
E

L

L

E
|
E

F

F

L

L

F

F

H

K

L

L

D

D

E

A

K

N

G

G

Q

N

P

P

T

T

I

E

T

L

H

T

D

D

Q

Q

A

G

S

G

Y

Y

F

F

D

D

L

F

S

A

P

P

I

L

D

D

L

L

G

G

E

Q

E

D

A

V

R

R

E

D

I

I

I

D

L

Y

T

A

L

L

E

E

K

H

M

M

G

G

F

F

E

Q

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

S

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

R

Y

F

S

G

D

D

A

V

L

L

D

C

V

T

A

A

D

D

K

N

I

V

M

V

T

T

F

F

K

K

M

Y

V

V

R

K

V

S

I

I

A

A

Q

Y

R

H

H

K

G

G

L

Y

H

Y

A

A

T

S

F

F

M

M

P

P

K

K

P

P

L

L

Y

F

G

G

V

V

N

N

G

G

S

S

G

G

M

M

H
|
H

T

S

N

N

L

Q

S

S

L

L

S

F

R

K

N

D

G

G

E

K

N

N

A

V

F

F

Y

Y

D

D

P

P

D

D

R

T

P

P

L

T

Q

K

L

L

S

S

E

Q

V

D

A

A

Y

M

Y

Y

F

Y

I

I

G

G

I

L

K

K

H

H

I

I

K

R

A

E

I

F

T

T

A

A

V

V

T

T

N

N

P

P

T

V

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

Y

Y

E

E

A

A

P

P

V

V

Y

Y

I

I

A

S

W

W

S

S

C

A

R

Q

N

N

R

R

S

S

P

S

L

L

I

I

R

R

I

I

P

P

A

A

K

T

R

R

G

G

P

N

S

G

T

T

R

R

I

I

E
|
E

L

L

R

R

S

C

P

P

D

D

P

P

S

A

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

A

L

I

M

I

L

K

R

A

A

G

G

L

L

D

E

G

G

I

I

K

K

N

N

K

K

I

I

E

D

P

P

P

G

P

E

P

P

V

T

D

N

R

V

N

N

I

I

Y

F

E

H

L

L

S

S

A

D

E

K

E

E

R

R

K

E

A

E

L

R

G

G

I

I

D

R

C

S

L

L

P

P

G

A

S

D

L

L

I

K

E

E

A

A

L

I

Q

D

E

E

L

M

E

K

K

G

D

S

E

K

V

F

I

V

K

K

E

E

A

A

L

L

G

G

P

E

H

H

I

V

Y

F

E

S

K

H

F

Y

M

L

E

C

A

A

K

K

L

E

K

M

E

E

W

W

H

D

E

E

Y

Y

S

K

T

A

R

V

V

V

H

H

P

P

W

W

E

E

I

L

E

S

T

R

Y

Y

L

L

binding magnesium ion: E132 (= E132), E134 (= E134), E189 (= E189), E196 (= E196), H245 (= H245), E333 (= E333)

8tfkA Glutamine synthetase (see paper)
61% identity, 98% coverage: 7:441/444 of query aligns to 3:437/440 of 8tfkA

query
sites
8tfkA

R

K

E

E

E

D

V

V

L

L

R

E

R

A

A

V

R

K

E

E

E

R

G

D

V

V

K

K

F

F

I

I

R

R

L

T

Q

Q

F

F

T

T

D

D

I

T

L

L

G

G

V

I

L

I

K

K

N

S

V

W

A

A

I

I

P

P

I

A

D

E

Q

Q

L

L

E

E

K

E

A

A

L

F

N

E

G

N

E

G

L

V

M

M

F

F

D

D

G

G

S

S

I

I

E

Q

G

G

F

F

V

T

R

R

I

I

E

E

S

S

D

D

M

M

Y

K

L

L

R

A

P

L

D

D

P

P

R

S

T

T

F

F

V

R

I

I

L

L

P

P

W

W

R

R

P

P

R

A

E

T

G

G

A

A

V

V

A

A

R

R

L

I

I

L

C

G

D

D

V

V

Y

Y

N

L

P

P

D

D

G

G

T

N

P

P

F

F

L

K

G

G

D

D

P

P

R

R

Y

Y

A

V

L

L

K

K

R

T

V

A

L

I

E

K

E

E

A

A

A

E

E

K

L

M

G

G

Y

F

T

S

C

M

N

N

A

V

G

G

P

P

E
|
E

L

L

E
|
E

F

F

L

L

F

F

H

K

L

L

D

D

E

A

K

N

G

G

Q

N

P

P

T

T

I

E

T

L

H

T

D

D

Q

Q

A

G

S

G

Y

Y

F

F

D

D

L

F

S

A

P

P

I

L

D

D

L

R

G

A

E

Q

E

D

A

V

R

R

E

D

I

I

I

D

L

Y

T

A

L

L

E

E

K

H

M

M

G

G

F

F

E

Q

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

S

Q

Q

H

H

E
|
E

I

I

D
|
D

F
|
F

K

R

Y
x
F

S

G

D

D

A

V

L

L

D

C

V

T

A

A

D

D

K

N

I

V

M

V

T

T

F

F

K

K

M

Y

V

V

R

K

V

S

I

I

A

A

Q

Y

R

H

H

K

G

G

L

Y

H

Y

A

A

T

S

F

F

M

M

P

P

K

K

P

P

L

L

Y

F

G

G

V

V

N

N

G
|
G

S

S

G

G

M

M

H
|
H

T

S

N
|
N

L

Q

S

S

L

L

S

F

R

K

N

D

G

G

E

K

N

N

A

V

F

F

Y

Y

D

D

P

P

D

D

R

T

P

P

L

T

Q

K

L

L

S

S

E

Q

V

D

A

A

Y

M

Y

Y

F

Y

I

I

G

G

I

L

K

K

H

H

I

I

K

R

A

E

I

F

T

T

A

A

V

V

T

T

N

N

P

P

T

V

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

V

V

Y

Y

I

I

A

S

W

W

S

S

C

A

R

Q

N

N

R
|
R

S

S

P

S

L

L

I

I

R
|
R

I

I

P

P

A

A

K

T

R

R

G

G

P

N

S
x
G

T

T

R
|
R

I

I

E
|
E

L

L

R
|
R

S

C

P

P

D

D

P

P

S

A

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

A

L

I

M

I

L

K

R

A

A

G

G

L

L

D

E

G

G

I

I

K

K

N

N

K

K

I

I

E

D

P

P

P

G

P

E

P

P

V

T

D

N

R

V

N

N

I

I

Y

F

E

H

L

L

S

S

A

D

E

K

E

E

R

R

K

E

A

E

L

R

G

G

I

I

D

R

C

S

L

L

P

P

G

A

S

D

L

L

I

K

E

E

A

A

L

I

Q

D

E

E

L

M

E

K

K

G

D

S

E

K

V

F

I

V

K

K

E

E

A

A

L

L

G

G

P

E

H

H

I

V

Y

F

E

S

K

H

F

Y

M

L

E

C

A

A

K

K

L

E

K

M

E

E

W

W

H

D

E

E

Y

Y

S

K

T

A

R

V

V

V

H

H

P

P

W

W

E

E

I

L

E

S

T

R

Y

Y

L

L

binding adenosine-5'-diphosphate: E128 (= E132), D194 (= D198), F195 (= F199), F197 (≠ Y201), N243 (= N247), R312 (= R316), R317 (= R321), G325 (≠ S329), R327 (= R331)
binding magnesium ion: E128 (= E132), E128 (= E132), E130 (= E134), E185 (= E189), E192 (= E196), E192 (= E196), H241 (= H245), E329 (= E333)
binding l-methionine-s-sulfoximine phosphate: E128 (= E132), E130 (= E134), E185 (= E189), E192 (= E196), G237 (= G241), H241 (= H245), R294 (= R298), E300 (= E304), R312 (= R316), R331 (= R335)

8oozA Glutamine synthetase (see paper)
61% identity, 99% coverage: 6:444/444 of query aligns to 2:430/430 of 8oozA

query
sites
8oozA

K

K

R

E

E

D

E

E

V

I

L

F

R

R

R

I

A

V

R

E

E

E

E

K

G

N

V

V

K

R

F

F

I

V

R

R

L

L

Q

Q

F

F

T

V

D

D

I

V

L

Q

G

G

V

I

L

P

K

K

N

N

V

V

A

A

I

I

P

P

I

V

D

G

Q

Q

L

L

E

E

K

K

A

A

L

L

N

G

G

P

E

G

L

I

M

H

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

-

V

-

R

-

I

-

E

-

S

S

D

D

M

M

Y

V

L

L

R

R

P

P

D

D

P

P

R

D

T

T

F

F

V

R

I

V

L

L

P

P

W

W

R

S

P

T

R

A

E

E

G

-

A

-

V

-

A

A

R

R

L

L

I

I

C

C

D

D

V

I

Y

E

N

L

P

P

D

D

G

G

T

K

P

P

F

F

L

M

G

G

D

C

P

P

R

R

Y

Q

A

V

L

L

K

K

R

K

V

N

L

M

E

E

A

A

E

K

L

L

G

G

Y

Y

T

V

C

M

N

N

A

T

G
|
G

P

P

E
|
E

L

M

E

E

F

F

L

L

F

F

H

K

L

-

D

R

E

Q

K

D

G

G

Q

M

P

P

T

T

T

N

I

I

T

P

H

Q

D

D

Q

R

A

G

S

G

Y

Y

F

F

D

D

L

L

S

A

P

P

I

I

D

D

L

L

G

A

E

E

A

I

R

K

R

R

E

E

I

I

I

V

L

L

T

V

L

L

E

E

K

E

M

M

G

G

F

F

E

E

I

V

E
|
E

A

A

S

A

H

H

E

E

V

V

A

A

P

F

G

G

Q

Q

H

H

E

E

I

I

D

D

F
|
F

K
|
K

Y
|
Y

S

D

D

N

A

A

L

L

D

A

V

T

A

A

D

D

K

N

I

V

M

I

T

T

F

L

K

K

M

Y

V

V

V

A

R

K

V

T

I

L

A

A

Q

L

R

Q

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

I

Y

F

G

G

V

V

N

N

G

G

S

S

G

G

M

M

H
|
H

T

T

N
|
N

L

T

S
|
S

L

L

S

F

R

K

N

D

G

G

E

K

N

N

A

A

F

F

Y

Y

D

D

P

P

D

D

R

A

P

P

L

D

Q

Q

L

I

S

S

E

D

V

T

A

L

Y

R

Y

Y

F

F

I

V

G

G

I

V

I

L

K

K

H

H

I

I

K

R

A

A

I

I

T

T

A

A

V

I

T

T

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

Y

Y

E

E

A

A

P

P

V

V

Y

Y

I

I

A

T

W

W

S

S

C

G

R

P

N

N

R

R

S

S

P

S

L

L

I

I

R

R

I

V

P

P

A

A

K

P

R

R

G

G

P

N

S
|
S

T

T

R
|
R

I

I

E
|
E

L

I

R

R

S

S

P

P

D

D

P

P

S

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

I

I

I

L

K

A

A

A

G

G

L

L

D

D

G

G

I

V

K

K

N

N

K

K

I

I

E

E

P

P

P

E

P

R

V

V

D

E

R

K

N

N

I

I

Y

Y

E

K

L

L

S

T

A

E

E

E

R

R

K

E

A

K

L

L

G

G

I

I

D

G

C

M

L

L

P

P

G

G

S

T

L

L

I

K

E

E

A

A

L

I

Q

E

E

C

L

F

E

K

K

E

D

D

E

E

V

L

I

L

K

V

E

S

A

A

L

L

G

G

P

E

H

H

I

V

Y

S

E

Q

K

S

F

I

M

I

E

N

A

V

K

A

L

M

K

A

E

D

W

W

H

D

E

S

Y

Y

S

R

T

T

R

Q

V

V

H

H

P

Q

W

W

E

E

I

L

E

D

T

R

Y

Y

L

L

V

Q

R

T

Y

Y

binding adenosine-5'-triphosphate: G117 (= G130), E170 (= E184), F185 (= F199), K186 (= K200), Y187 (= Y201), N233 (= N247), S235 (= S249), S315 (= S329), R317 (= R331)
binding magnesium ion: E119 (= E132), H231 (= H245), E319 (= E333)

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
56% identity, 98% coverage: 8:441/444 of query aligns to 3:444/446 of 8ooqB

query
sites
8ooqB

E

E

E

Q

V

V

L

L

R

E

R

Y

A

V

R

K

E

S

E

N

G

N

V

V

K

K

F
|
F

I

M

R
|
R

L

F

Q

Q

F

F

T

V

D

D

I

I

L

L

G

G

V

V

L

P

K

K

N

N

V

V

A
|
A

I

F

P

P

I

I

-

K

-

A

-

G

-

E

-

K

-

G

-

I

D

E

Q

E

L

L

E

R

K

D

A

V

L

L

N

E

G

N

E

G

L

L

M

Y

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

G

I

I

E

N

E

E

S

S

D

D

M

M

Y

M

L

L

R

K

P

P

D

D

P

L

R

S

T

T

F

F

V

S

I

V

L

L

P

P

W

W

R
|
R

P

P

R

S

E

E

G

K

A

S

V
|
V

A

A

R

R

L

V

I

I

C

C

D

D

V

V

Y

Y

N

T

P

T

D

K

G

G

T

K

P

P

F

F

L

E

G

G

D

D

P

P

R

R

Y

G

A

C

L

L

K

K

R

R

V

V

L

M

E

E

-

F

A

K

E

E

L

F

G

N

Y

G

T

E

C

Y

N

F

A

V

G

G

P

P

E

E

L

P

E
|
E

F

F

L

L

F

L

H

K

L

K

D

D

E

P

K

H

G

N

Q

P

P

H

T

K

T

Y

I

I

T

P

H

A

D

D

Q

D

A

G

S

G

Y

Y

F

F

D

D

L

L

S

E

P

P

I

M

D

D

L

E

G

A

E
x
P

E

D

A

I

R
|
R

E

D

I

I

I

V

L

F

T

A

L

L

E

E

K

N

M

L

G

G

F

F

E

H

I

V

E

E

A

A

S
|
S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

V

D

D

F

F

K

K

Y

F

S

D

D

D

A

A

L

L

D

K

V

T

A

A

D

D

K

S

I

V

M

I

T

T

F

F

K

K

M

T

V

T

V

I

R

K

V

T

I

I

A

A

Q

E

R

Q

H

H

G

G

L

L

H

K

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

F

Y

F

G

G

V

M

N

N

G

G

S

S

G

G

M

M

H

H

T

C

N

H

L

Q

S

S

L

I

S

W

R

L

N

N

G

G

E

E

N

P

A

S

F

F

Y

Y

D

D

P

E

D

N

R

A

P

P

L

Y

Q

Q

L

L

S

S

E

E

V

T

A

C

Y

M

Y

N

F

Y

I

V

G

A

G

G

I

I

I

L

K

K

H

H

I

A

K

K

A

A

I

I

T

V

A

A

V

I

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

Y

Y

E

E

A

A

P

P

V

V

Y

N

I

I

A

A

W

W

S

A

C

N

R

S

N

N

R

R

S

S

P

A

L

I

I

I

R

R

I

V

P

P

A

A

K

A

R

R

G

G

P

K

S

G

T

T

R

R

I

I

E

E

L

F

R

R

S

A

P

P

D

D

P

P

S

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

T

A

V

I

M

I

L

K

A

A

A

G

G

L

L

D

D

G

G

I

V

K

K

N

N

K

K

I

L

E

D

P

A

P

P

P

E

P

P

V

V

D

E

R

R

N

N

I

I

Y

F

E

A

L

M

S

S

A

E

E

A

E

E

R

K

K

K

A

E

L

L

G

G

I

I

D

E

C

S

L

V

P

P

G

A

S

N

L

L

I

K

E

A

A

A

L

L

Q

D

E

E

L

L

E

E

K

N

D

N

E

D

V

V

I

L

K

K

E

N

A

A

L

L

G

G

P

K

H

H

I

I

Y

F

E

E

K

S

F

F

M

L

E

E

A

I

K

K

L

N

K

A

E

E

W

W

H

D

E

S

Y

F

S

R

T

T

R

S

V

V

H

T

P

D

W

W

E

E

I

T

E

T

T

A

Y

Y

L

L

binding 2-oxoglutaric acid: F16 (= F21), R18 (= R23), A32 (= A37), R86 (= R84), V92 (= V90), P169 (≠ E166), R172 (= R169), R173 (= R170), S189 (= S186)
binding magnesium ion: E137 (= E134), E192 (= E189), E199 (= E196)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
56% identity, 98% coverage: 8:441/444 of query aligns to 4:445/447 of 8oooA

query
sites
8oooA

E

E

E

Q

V

V

L

L

R

E

R

Y

A

V

R

K

E

S

E

N

G

N

V

V

K

K

F
|
F

I

M

R
|
R

L

F

Q

Q

F

F

T

V

D

D

I

I

L

L

G

G

V

V

L

P

K

K

N

N

V

V

A
|
A

I

F

P

P

I

I

-

K

-

A

-

G

-

E

-

K

-

G

-

I

D

E

Q

E

L

L

E

R

K

D

A

V

L

L

N

E

G

N

E

G

L

L

M

Y

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

G

I

I

E

N

E

E

S

S

D

D

M

M

Y

M

L

L

R

K

P

P

D

D

P

L

R

S

T

T

F

F

V

S

I

V

L

L

P

P

W

W

R
|
R

P

P

R

S

E

E

G

K

A

S

V
|
V

A

A

R

R

L

V

I

I

C

C

D

D

V

V

Y

Y

N

T

P

T

D

K

G

G

T

K

P

P

F

F

L

E

G

G

D

D

P

P

R

R

Y

G

A

C

L

L

K

K

R

R

V

V

L

M

E

E

-

F

A

K

E

E

L

F

G

N

Y

G

T

E

C

Y

N

F

A

V

G

G

P

P

E
|
E

L

P

E

E

F

F

L

L

F

L

H

K

L

K

D

D

E

P

K

H

G

N

Q

P

P

H

T

K

T

Y

I

I

T

P

H

A

D

D

Q

D

A

G

S

G

Y

Y

F

F

D

D

L

L

S

E

P

P

I

M

D

D

L

E

G

A

E
x
P

E

D

A

I

R
|
R

E

D

I

I

I

V

L

F

T

A

L

L

E

E

K

N

M

L

G

G

F

F

E

H

I

V

E
|
E

A

A

S
|
S

H

H

E

E

V

V

A

A

P

P

G

G

Q

Q

H

H

E

E

I

V

D

D

F
|
F

K
|
K

Y
x
F

S

D

D

D

A

A

L

L

D

K

V

T

A

A

D

D

K

S

I

V

M

I

T

T

F

F

K

K

M

T

V

T

V

I

R

K

V

T

I

I

A

A

Q

E

R

Q

H

H

G

G

L

L

H

K

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

F

Y

F

G

G

V

M

N

N

G

G

S

S

G

G

M

M

H

H

T

C

N
x
H

L

Q

S
|
S

L

I

S

W

R

L

N

N

G

G

E

E

N

P

A

S

F

F

Y

Y

D

D

P

E

D

N

R

A

P

P

L

Y

Q

Q

L

L

S

S

E

E

V

T

A

C

Y

M

Y

N

F

Y

I

V

G

A

G

G

I

I

I

L

K

K

H

H

I

A

K

K

A

A

I

I

T

V

A

A

V

I

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

Y

Y

E

E

A

A

P

P

V

V

Y

N

I

I

A

A

W

W

S

A

C

N

R

S

N

N

R

R

S

S

P

A

L

I

I

I

R
|
R

I

V

P

P

A

A

K

A

R

R

G

G

P

K

S

G

T

T

R
|
R

I

I

E

E

L

F

R

R

S

A

P

P

D

D

P

P

S

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

T

A

V

I

M

I

L

K

A

A

A

G

G

L

L

D

D

G

G

I

V

K

K

N

N

K

K

I

L

E

D

P

A

P

P

P

E

P

P

V

V

D

E

R

R

N

N

I

I

Y

F

E

A

L

M

S

S

A

E

E

A

E

E

R

K

K

K

A

E

L

L

G

G

I

I

D

E

C

S

L

V

P

P

G

A

S

N

L

L

I

K

E

A

A

A

L

L

Q

D

E

E

L

L

E

E

K

N

D

N

E

D

V

V

I

L

K

K

E

N

A

A

L

L

G

G

P

K

H

H

I

I

Y

F

E

E

K

S

F

F

M

L

E

E

A

I

K

K

L

N

K

A

E

E

W

W

H

D

E

S

Y

F

S

R

T

T

R

S

V

V

H

T

P

D

W

W

E

E

I

T

E

T

T

A

Y

Y

L

L

binding 2-oxoglutaric acid: F17 (= F21), R19 (= R23), A33 (= A37), R87 (= R84), V93 (= V90), P170 (≠ E166), R173 (= R169), R174 (= R170), S190 (= S186)
binding adenosine-5'-triphosphate: E136 (= E132), E188 (= E184), F203 (= F199), K204 (= K200), F205 (≠ Y201), H251 (≠ N247), S253 (= S249), R325 (= R321), R335 (= R331)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
47% identity, 98% coverage: 6:441/444 of query aligns to 4:444/446 of A0R083

query
sites
A0R083

K

Q

R

K

E

E

E

F

V

V

L

L

R

R

R

T

A

L

R

E

E

E

E

R

G

D

V

I

K

R

F

F

I

V

R

R

L

L

Q

W

F

F

T

T

D

D

I

V

L

L

G

G

V

Y

L

L

K

K

N

S

V

V

A

A

I

I

P

A

I

P

D

A

Q

E

L

L

E

E

K

G

A

A

L

F

N

E

G

E

E

G

L

I

M

G

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

A

R

R

I

V

E

F

E

E

S

S

D

D

M

T

Y

V

L

A

R

R

P

P

D

D

P

P

R

S

T

T

F

F

V

Q

I

V

L

L

P

P

W

W

R

K

P

T

R

S

E

D

G

G

A

N

-

H

-

Y

V

S

A

A

R

R

L

M

I

F

C

C

D

D

V

I

Y

T

N

M

P

P

D

D

G

G

T

S

P

P

F

S

L

W

G

A

D

D

P

S

R

R

Y

H

A

V

L

L

K

R

R

R

V

Q

L

L

E

A

E

K

A

A

A

S

E

D

L

L

G

G

Y

F

T

T

C

C

N

Y

A

V

G

H

P

P

E

E

L

I

E

E

F

F

L

L

F

L

H

K

L

P

D

G

E

P

K

N

G

D

Q

G

P

T

T

P

I

E

T

P

H

A

D

D

Q

N

A

G

S

G

Y

Y

F

F

D

D

L

Q

S

A

P

V

I

H

D

D

L

A

G

A

E

P

E

N

A

F

R

R

E

H

I

A

I

I

L

E

T

A

L

L

E

E

K

Q

M

M

G

G

F

I

E

S

I

V

E

E

A

F

S

S

H

H

E

E

V

G

A

A

P

P

G

G

Q

Q

H

Q

E

E

I

I

D

D

F

L

K

R

Y

Y

S

A

D

D

A

A

L

L

D

S

V

M

A

A

D

D

K

N

I

V

M

M

T

T

F

F

K

R

M

Y

V

L

V

V

R

K

V

E

I

V

A

A

Q

L

R

A

H

D

G

G

L

V

H

R

A

A

T

S

F

F

M

M

P

P

K

K

P

P

L

F

Y

A

G

E

V

H

N

P

G

G

S

S

G

A

M

M

H

H

T

T

N

H

L

M

S

S

L

L

S

F

R

E

N

G

G

D

E

T

N

N

A

A

F

F

Y

H

D

S

P

P

D

D

R

D

P

P

L

L

Q

Q

L

L

S

S

E

D

V

V

A

A

Y

K

Y

S

F

F

I

I

G

A

G

G

I

I

I

L

K

E

H

H

I

A

K

N

A

E

I

I

T

S

A

A

V

V

T

T

N

N

P

Q

T

W

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

H

G

G

Y

G

E

E

A

A

P

P

V

T

Y

A

I

A

A

S

W

W

S

G

C

A

R

A

N

N

R

R

S

S

P

A

L

L

I

V

R

R

I

V

P

P

A

M

-

Y

-

T

-

P

K

H

R

K

G

V

P

S

S

S

T

R

R

R

I

V

E

E

L

V

R

R

S

S

P

P

D

D

P

S

S

A

C

C

N

N

P

P

Y

Y

L

L

A

T

L

F

A

A

A

V

I

L

K

A

A

A

G

G

L

L

D

R

G

G

I

V

K

E

N
x
K

K

G

I

Y

E

V

P

L

P

G

P

P

P

Q

V

A

D

E

R

D

N

N

I

V

Y

W

E

S

L

L

S

T

A

Q

E

E

R

R

K

R

A

A

L

M

G

G

I

Y

D

R

C

E

L

L

P

P

G

T

S

S

L

L

I

G

E

N

A

A

L

L

Q

E

E

S

L

M

E

E

K

N

D

S

E

E

V

L

I

V

K

A

E

E

A

A

L

L

G

G

P

E

H

H

I

V

Y

F

E

D

K

Y

F

F

M

L

E

R

A

N

K

K

L

R

K

S

E

E

W

W

H

E

E

N

Y

Y

S

R

T

S

R

H

V

V

H

T

P

P

W

Y

E

E

I

L

E

K

T

N

Y

Y

L

L

K363 (≠ N360) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
47% identity, 98% coverage: 6:441/444 of query aligns to 4:444/446 of P9WN37

query
sites
P9WN37

K

Q

R

K

E

E

E

F

V

V

L

L

R

R

R

T

A

L

R

E

E

E

E

R

G

D

V

I

K

R

F

F

I

V

R

R

L

L

Q

W

F

F

T

T

D

D

I

V

L

L

G

G

V

F

L

L

K

K

N

S

V

V

A

A

I

I

P

A

I

P

D

A

Q

E

L

L

E

E

K

G

A

A

L

F

N

E

G

E

E

G

L

I

M

G

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

A

R

R

I

V

E

S

E

E

S

S

D

D

M

T

Y

V

L

A

R

H

P

P

D

D

P

P

R

S

T

T

F

F

V

Q

I

V

L

L

P

P

W

W

R

A

P

T

R

S

E

S

G

G

A

H

-

H

V

S

A

A

R

R

L

M

I

F

C

C

D

D

V

I

Y

T

N

M

P

P

D

D

G

G

T

S

P

P

F

S

L

W

G

A

D

D

P

P

R

R

Y

H

A

V

L

L

K

R

R

R

V

Q

L

L

E

T

E

K

A

A

A

G

E

E

L

L

G

G

Y

F

T

S

C

C

N

Y

A

V

G

H

P

P

E

E

L

I

E

E

F

F

L

L

F

L

H

K

L

P

D

G

E

P

K

E

G

D

Q

G

P

S

T

V

T

P

I

V

T

P

H

V

D

D

Q

N

A

A

S

G

Y

Y

F

F

D

D

L

Q

S

A

P

V

I

H

D

D

L

S

G

A

E

L

E

N

A

F

R

R

E

H

I

A

I

I

L

D

T

A

L

L

E

E

K

F

M

M

G

G

F

I

E

S

I

V

E

E

A

F

S

S

H

H

E

E

V

G

A

A

P

P

G

G

Q

Q

H

Q

E

E

I

I

D

D

F

L

K

R

Y

F

S

A

D

D

A

A

L

L

D

S

V

M

A

A

D

D

K

N

I

V

M

M

T

T

F

F

K

R

M

Y

V

V

V

I

R

K

V

E

I

V

A

A

Q

L

R

E

H

E

G

G

L

A

H

R

A

A

T

S

F

F

M

M

P

P

K

K

P

P

L

F

Y

G

G

Q

V

H

N

P

G

G

S

S

G

A

M

M

H

H

T

T

N

H

L

M

S

S

L

L

S

F

R

E

N

G

G

D

E

V

N

N

A

A

F

F

Y

H

D

S

P

A

D

D

R

D

P

P

L

L

Q

Q

L

L

S

S

E

E

V

V

A

G

Y

K

Y

S

F

F

I

I

G

A

G

G

I

I

I

L

K

E

H

H

I

A

K

C

A

E

I

I

T

S

A

A

V

V

T

T

N

N

P

Q

T

W

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

Q

G

G

Y

G

E

E

A

A

P

P

V

T

Y

A

I

A

A

S

W

W

S

G

C

A

R

A

N

N

R

R

S

S

P

A

L

L

I

V

R

R

I

V

P

P

A

M

-

Y

-

T

-

P

K

H

R

K

G

T

P

S

S

S

T

R

R

R

I

V

E

E

L

V

R

R

S

S

P

P

D

D

P

S

S

A

C

C

N

N

P

P

Y

Y

L

L

A

T

L

F

A

A

A

V

I

L

K

A

A

A

G

G

L

L

D

R

G

G

I

V

K

E

N
x
K

K

G

I

Y

E

V

P

L

P

G

P

P

P

Q

V

A

D

E

R

D

N

N

I

V

Y

W

E

D

L

L

S

T

A

P

E

E

R

R

K

R

A

A

L

M

G

G

I

Y

D

R

C

E

L

L

P

P

G

S

S

S

L

L

I

D

E

S

A

A

L

L

Q

R

E

A

L

M

E

E

K

A

D

S

E

E

V

L

I

V

K

A

E

E

A

A

L

L

G

G

P

E

H

H

I

V

Y

F

E

D

K

F

F

F

M

L

E

R

A

N

K

K

L

R

K

T

E

E

W

W

H

A

E

N

Y

Y

S

R

T

S

R

H

V

V

H

T

P

P

W

Y

E

E

I

L

E

R

T

T

Y

Y

L

L

K363 (≠ N360) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P0A9C5 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Escherichia coli (strain K12) (see 3 papers)
44% identity, 98% coverage: 5:440/444 of query aligns to 1:466/469 of P0A9C5

query
sites
P0A9C5

M
|
M

K

S

R

A

E

E

E

H

V

V

L

L

R

T

R

M

A

L

R

N

E

E

E

H

G

E

V

V

K

K

F

F

I

V

R

D

L

L

Q

R

F

F

T

T

D

D

I

T

L

K

G

G

V

K

L

E

K

Q

N

H

V

V

A

T

I

I

P

P

I

A

D

H

Q

Q

L

V

E

N

K

A

A

E

L

F

N

F

G

E

E

E

-

G

L

K

M

M

F

F

D

D

G

G

S

S

I

I

E

G

G

G

F

W

V

K

R

G

I

I

E

N

E

E

S

S

D

D

M

M

Y

V

L

L

R

M

P

P

D

D

P

A

R

S

T

T

F

A

V

V

I

I

L

D

P

P

W

F

R

F

P

A

R

D

E

S

G

T

A

L

V

I

A

I

R

R

L

-

I

-

C

C

D

D

V

I

Y

L

N

E

P

P

D

-

G

G

T

T

-

L

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Y

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D

G

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D

D

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A

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K

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D

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Y

A

L

A

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G

G

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I

-

A

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D

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G

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P

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F

F

L

L

F

F

H

D

L

D

D

I

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K

F

G

G

Q

S

P

S

T

I

T

S

I

G

T

S

H

H

D

V

-

A

-

I

-

D

-

I

-

E

-

G

-

A

-

W

-

N

-

S

-

T

-

Q

-

Y

-

E

-

G

-

N

-

K

-

G

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H

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-

A

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Q

K

A

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G

Y

Y

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F

M

M

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K

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F

G

G

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D

N

N

G

G

S

S

G

G

M

M

H

H

T

C

N

H

L

M

S

S

L

L

S

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N

N

G

G

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N

A

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A

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N

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M

A

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G

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L

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N

N

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D

D

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N

N

I

L

Y
|
Y

E

D

L

L

S

P

A

P

E

E

R

A

K

K

A

E

L

-

G

-

I

I

D

P

C

Q

L

V

P

A

G

G

S

S

L

L

I

E

E

E

A

A

L

L

Q

N

E

E

L

L

E

D

K

L

D

D

E

R

V

E

I

F

K

L

E

K

A

A

L

G

G

G

P

V

H

F

I

T

Y

D

E

E

K

A

F

-

M

I

E

D

A

A

K

Y

L

I

K

A

E

L

W

R

H

R

E

E

Y

E

S

D

T

D

R

R

V

V

-

R

-

M

-

T

-

P

H

H

P

P

W

V

E

E

I

F

E

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T

L

Y

Y

M1 (= M5) modified: Initiator methionine, Removed
Y398 (= Y373) modified: O-AMP-tyrosine

1fpyA Crystal structure of glutamine synthetase from salmonella typhimurium with inhibitor phosphinothricin (see paper)
44% identity, 98% coverage: 8:440/444 of query aligns to 3:465/468 of 1fpyA

query
sites
1fpyA

E

E

E

H

V

V

L

L

R

T

R

M

A

L

R

N

E

E

E

H

G

E

V

V

K

K

F

F

I

V

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D

L

L

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R

F

F

T

T

D

D

I

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G

G

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K

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K

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N

H

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V

A

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I

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P

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D

H

Q

Q

L

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N

K

A

A

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L

F

N

F

G

E

E

E

-

G

L

K

M

M

F

F

D

D

G

G

S

S

I

I

E

G

G

G

F

W

V

K

R

G

I

I

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N

E

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S

S

D

D

M

M

Y

V

L

L

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P

D

D

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F

A

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V

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W

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A

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A

L

V

I

A

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-

I

-

C

C

D

D

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Y

L

N

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P

P

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-

G

G

T

T

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L

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A

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A

K

K

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-

Y

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A

A

A

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G

L

I

G

A

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D

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V

N

L

A

F

G
|
G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

H

D

L

D

D

I

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K

F

G

G

Q

A

P

S

T

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S

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S

H

H

D

V

-

A

-

I

-

D

-

I

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E

-

G

-

A

-

W

-

N

-

S

-

T

-

K

-

Y

-

E

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G

-

N

-

K

-

G

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H

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Q

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A

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G

Y

Y

F

F

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A

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L

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M

G

G

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L

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I

V

E
|
E

A

A

S

H

H

H

E
|
E

V

V

A

A

-

T

P

A

G

G

Q

Q

H

N

E
|
E

I

V

D

A

F
x
T

K

R

Y
x
F

S

N

D

T

A

M

L

T

D

K

V

K

A

A

D

D

K

E

I

I

M

Q

T

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Y

K

K

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Y

V

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N

I

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A

A

Q

H

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G

G

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K

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A

A

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T

F

F

M

M

P

P

K

K

P

P

L

M

Y

F

G

G

V

D

N

N

G
|
G

S

S

G

G

M

M

H
|
H

T

C

N
x
H

L

M

S
|
S

L

L

S

A

R

K

N

N

G

G

E

T

N

N

A

L

F

F

Y

-

D

S

P

G

D

D

R

K

P

Y

L

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A

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L

Y

Y

F

Y

I

I

G

G

I

V

I

I

K

K

H

H

I

A

K

K

A

A

I

I

T

N

A

A

V

L

T

A

N

N

P

P

T

T

V

T

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

V

V

Y

M

I

L

A

A

W

Y

S

S

C

A

R

R

N

N

R

R

S

S

P

A

L

S

I

I

R

R

I

I

P

P

A

V

K

V

R

A

G

S

P

P

-

K

S

A

T

R

R
|
R

I

I

E
|
E

L

V

R
|
R

S

F

P

P

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

C

L

F

A

A

I

L

K

M

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

N

K

K

I

I

E

H

P

P

P

G

P

E

P

P

V

M

D

D

R

K

N

N

I

L

Y

Y

E

D

L

L

S

P

A

P

E

E

R

A

K

K

A

E

L

-

G

-

I

I

D

P

C

Q

L

V

P

A

G

G

S

S

L

L

I

E

E

E

A

A

L

L

Q

N

E

A

L

L

E

D

K

L

D

D

E

R

V

E

I

F

K

L

E

K

A

A

L

G

G

G

P

V

H

F

I

T

Y

D

E

E

K

A

F

-

M

I

E

D

A

A

K

Y

L

I

K

A

E

L

W

R

H

R

E

E

Y

E

S

D

T

D

R

R

V

V

-

R

-

M

-

T

-

P

H

H

P

P

W

V

E

E

I

F

E

E

T

L

Y

Y

binding adenosine-5'-diphosphate: G127 (= G130), E129 (= E132), E207 (= E184), T223 (≠ F199), F225 (≠ Y201), H271 (≠ N247), S273 (= S249), R355 (= R331), E357 (= E333)
binding manganese (ii) ion: E129 (= E132), E131 (= E134), E212 (= E189), E220 (= E196), H269 (= H245), E357 (= E333)
binding phosphinothricin: E131 (= E134), E212 (= E189), G265 (= G241), H269 (= H245), R321 (= R298), E327 (= E304), R359 (= R335)

Query Sequence

>WP_015738418.1 NCBI__GCF_000024605.1:WP_015738418.1
MEELMKREEVLRRAREEGVKFIRLQFTDILGVLKNVAIPIDQLEKALNGELMFDGSSIEG
FVRIEESDMYLRPDPRTFVILPWRPREGAVARLICDVYNPDGTPFLGDPRYALKRVLEEA
AELGYTCNAGPELEFFLFHLDEKGQPTTITHDQASYFDLSPIDLGEEARREIILTLEKMG
FEIEASHHEVAPGQHEIDFKYSDALDVADKIMTFKMVVRVIAQRHGLHATFMPKPLYGVN
GSGMHTNLSLSRNGENAFYDPDRPLQLSEVAYYFIGGIIKHIKAITAVTNPTVNSYKRLV
PGYEAPVYIAWSCRNRSPLIRIPAKRGPSTRIELRSPDPSCNPYLALAAIIKAGLDGIKN
KIEPPPPVDRNIYELSAEERKALGIDCLPGSLIEALQELEKDEVIKEALGPHIYEKFMEA
KLKEWHEYSTRVHPWEIETYLVRY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory