SitesBLAST
Comparing WP_015739275.1 NCBI__GCF_000024605.1:WP_015739275.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 5 hits to proteins with known functional sites (download)
1dbfA Chorismate mutase from bacillus subtilis at 1.30 angstrom (see paper)
49% identity, 90% coverage: 6:128/137 of query aligns to 3:126/127 of 1dbfA
- active site: R7 (= R10), R63 (= R66), C75 (= C77), E78 (= E80), R90 (= R92), Y108 (= Y110), R116 (= R118)
- binding glycerol: R7 (= R10), K25 (= K28), E64 (= E67), Q103 (≠ E105), Y108 (= Y110), L115 (= L117), L121 (= L123)
- binding sulfate ion: E13 (≠ K16), R63 (= R66), T74 (≠ L76), C75 (= C77), E78 (= E80), R90 (= R92), K111 (≠ E113)
P19080 Chorismate mutase AroH; CM; EC 5.4.99.5 from Bacillus subtilis (strain 168) (see 2 papers)
48% identity, 90% coverage: 6:128/137 of query aligns to 3:126/127 of P19080
- R7 (= R10) binding prephenate
- TCMQE 74:78 (≠ LCARE 76:80) binding prephenate
- E78 (= E80) mutation to A: No chorismate mutase activity.
- R90 (= R92) binding prephenate; mutation to A: No chorismate mutase activity.; mutation to G: 2-fold decrease in affinity and very low decrease in catalytic efficiency.; mutation to K: Low decrease in catalytic efficiency and in affinity.
- Y108 (= Y110) binding prephenate
3zp7A Arg90cit chorismate mutase of bacillus subtilis in complex with chorismate and prephenate (see paper)
50% identity, 83% coverage: 6:119/137 of query aligns to 3:117/117 of 3zp7A
- active site: R7 (= R10), R63 (= R66), C75 (= C77), E78 (= E80), V107 (≠ L109), L115 (= L117)
- binding (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid: R7 (= R10), F57 (= F60), A59 (= A62), K60 (≠ L63), R63 (= R66), V73 (≠ L75), T74 (≠ L76), C75 (= C77), E78 (= E80), R90 (= R92), L115 (= L117)
- binding prephenic acid: R7 (= R10), F57 (= F60), A59 (= A62), R63 (= R66), V73 (≠ L75), T74 (≠ L76), C75 (= C77), E78 (= E80), R90 (= R92), Y108 (= Y110), L115 (= L117)
2chtA Crystal structures of the monofunctional chorismate mutase from bacillus subtilis and its complex with a transition state analog (see paper)
49% identity, 82% coverage: 6:117/137 of query aligns to 2:114/114 of 2chtA
- active site: R6 (= R10), R62 (= R66), C74 (= C77), E77 (= E80), R89 (= R92), Y107 (= Y110)
- binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R6 (= R10), F56 (= F60), A58 (= A62), K59 (≠ L63), R62 (= R66), V72 (≠ L75), T73 (≠ L76), C74 (= C77), R89 (= R92), Y107 (= Y110), L114 (= L117)
1comA The monofunctional chorismate mutase from bacillus subtilis: structure determination of chorismate mutase and its complexes with a transition state analog and prephenate, and implications on the mechanism of enzymatic reaction (see paper)
49% identity, 82% coverage: 6:117/137 of query aligns to 2:114/114 of 1comA
- active site: R6 (= R10), R62 (= R66), C74 (= C77), E77 (= E80), R89 (= R92), Y107 (= Y110)
- binding prephenic acid: R6 (= R10), F56 (= F60), A58 (= A62), V72 (≠ L75), T73 (≠ L76), C74 (= C77), E77 (= E80), R89 (= R92)
Query Sequence
>WP_015739275.1 NCBI__GCF_000024605.1:WP_015739275.1
MVLIPVRGIRGAITVKENTREAILEATKELLLALVRENKLAVEDIAGIFFTLTPDLNAEF
PALAARELGWHYVPLLCAREIDVPGSLPRVIRVLLLANTEKSQEEIKHLYLREAAVLRPD
LALPQNSQQEERGDGTK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory