SitesBLAST
Comparing WP_015739363.1 NCBI__GCF_000024605.1:WP_015739363.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 12 hits to proteins with known functional sites (download)
6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
33% identity, 80% coverage: 15:215/251 of query aligns to 21:210/291 of 6n2oD
- binding magnesium ion: D101 (= D94), N129 (= N122), I131 (= I124)
- binding succinyl-coenzyme a: I57 (≠ V50), R62 (≠ F55), L134 (≠ M127), K136 (≠ G129)
- binding iron/sulfur cluster: W24 (≠ Y18), C25 (= C19), C28 (= C22), C59 (= C52), C208 (= C213), T210 (= T215)
- binding thiamine diphosphate: I57 (≠ V50), G58 (= G51), C59 (= C52), S60 (≠ A53), H76 (= H69), G102 (= G95), D103 (= D96), N129 (= N122), I131 (= I124), G133 (= G126), L134 (≠ M127), T135 (= T128)
Sites not aligning to the query:
6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
33% identity, 80% coverage: 15:215/251 of query aligns to 21:210/291 of 6n2oB
- binding 2-oxoglutaric acid: R62 (≠ F55), L134 (≠ M127)
- binding coenzyme a: K136 (≠ G129), Y150 (≠ T143)
- binding magnesium ion: D101 (= D94), N129 (= N122), I131 (= I124)
- binding iron/sulfur cluster: W24 (≠ Y18), C25 (= C19), C28 (= C22), C59 (= C52), C208 (= C213), T210 (= T215)
- binding thiamine diphosphate: I57 (≠ V50), G58 (= G51), C59 (= C52), S60 (≠ A53), H76 (= H69), G102 (= G95), D103 (= D96), N129 (= N122), I131 (= I124), Y132 (= Y125), G133 (= G126), L134 (≠ M127), T135 (= T128)
Sites not aligning to the query:
6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
33% identity, 80% coverage: 15:215/251 of query aligns to 21:210/291 of 6n2nB
- binding magnesium ion: D101 (= D94), N129 (= N122), I131 (= I124)
- binding iron/sulfur cluster: W24 (≠ Y18), C25 (= C19), C28 (= C22), H30 (= H24), C59 (= C52), C208 (= C213), T210 (= T215)
- binding thiamine diphosphate: I57 (≠ V50), G58 (= G51), C59 (= C52), S60 (≠ A53), H76 (= H69), G100 (= G93), D101 (= D94), G102 (= G95), D103 (= D96), N129 (= N122), I131 (= I124), Y132 (= Y125), G133 (= G126), L134 (≠ M127), T135 (= T128)
Sites not aligning to the query:
5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
33% identity, 92% coverage: 18:249/251 of query aligns to 8:228/301 of 5b46B
- binding magnesium ion: D87 (= D94), N115 (= N122), V117 (≠ I124)
- binding iron/sulfur cluster: W8 (≠ Y18), C9 (= C19), C12 (= C22), C43 (= C52), C194 (= C213), T196 (= T215), Y197 (≠ N216)
- binding thiamine diphosphate: I41 (≠ V50), G42 (= G51), C43 (= C52), S44 (≠ A53), H62 (= H69), G86 (= G93), G88 (= G95), D89 (= D96), N115 (= N122), V117 (≠ I124), Y118 (= Y125), G119 (= G126), L120 (≠ M127), T121 (= T128)
Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
33% identity, 92% coverage: 18:249/251 of query aligns to 11:231/304 of Q96XT4
- C12 (= C19) binding [4Fe-4S] cluster
- C15 (= C22) binding [4Fe-4S] cluster
- IGCS 44:47 (≠ VGCA 50:53) binding thiamine diphosphate
- C46 (= C52) binding [4Fe-4S] cluster
- D90 (= D94) binding Mg(2+)
- GD 91:92 (= GD 95:96) binding thiamine diphosphate
- N118 (= N122) binding Mg(2+)
- V120 (≠ I124) binding Mg(2+)
- GL 122:123 (≠ GM 126:127) binding thiamine diphosphate
- C197 (= C213) binding [4Fe-4S] cluster
Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
32% identity, 89% coverage: 18:241/251 of query aligns to 11:222/305 of Q96Y68
- C12 (= C19) binding [4Fe-4S] cluster
- C15 (= C22) binding [4Fe-4S] cluster
- C46 (= C52) binding [4Fe-4S] cluster
- K49 (≠ F55) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
- D90 (= D94) binding Mg(2+)
- GD 91:92 (= GD 95:96) binding thiamine diphosphate
- N118 (= N122) binding Mg(2+)
- V120 (≠ I124) binding Mg(2+)
- GL 122:123 (≠ GM 126:127) binding thiamine diphosphate
- K125 (≠ G129) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
- K173 (≠ I185) mutation to A: Same oxidoreductase activity as the wild-type.
- C197 (= C213) binding [4Fe-4S] cluster
P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
32% identity, 89% coverage: 18:241/251 of query aligns to 11:222/305 of P72579
- K49 (≠ F55) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
- L123 (≠ M127) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.
5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
30% identity, 90% coverage: 18:244/251 of query aligns to 4:222/275 of 5b47B
- binding magnesium ion: D83 (= D94), N111 (= N122)
- binding iron/sulfur cluster: C5 (= C19), C8 (= C22), C39 (= C52), C179 (= C213)
- binding thiamine diphosphate: I37 (≠ V50), G38 (= G51), C39 (= C52), S40 (≠ A53), H58 (= H69), G84 (= G95), D85 (= D96), N111 (= N122), V113 (≠ I124), Y114 (= Y125), L116 (≠ M127)
5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
33% identity, 79% coverage: 18:215/251 of query aligns to 7:185/286 of 5b48B
- binding magnesium ion: D84 (= D94), N112 (= N122), V114 (≠ I124)
- binding iron/sulfur cluster: C8 (= C19), C11 (= C22), C42 (= C52), C183 (= C213), T185 (= T215)
- binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (≠ V50), G41 (= G51), C42 (= C52), S43 (≠ A53), H59 (= H69), G85 (= G95), D86 (= D96), N112 (= N122), V114 (≠ I124), Y115 (= Y125), G116 (= G126), L117 (≠ M127)
5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
24% identity, 92% coverage: 19:249/251 of query aligns to 24:267/310 of 5exdF
- active site: N143 (≠ M127)
- binding magnesium ion: D110 (= D94), N138 (= N122), S140 (≠ I124)
- binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ V50), G51 (= G51), C52 (= C52), I74 (≠ H69), D110 (= D94), G111 (= G95), Y136 (≠ V120), N138 (= N122), S140 (≠ I124), Y141 (= Y125), A142 (≠ G126), N143 (≠ M127), T144 (= T128)
- binding iron/sulfur cluster: C24 (= C19), C27 (= C22), P29 (≠ H24), C52 (= C52), A142 (≠ G126), C225 (= C213), K227 (≠ T215)
5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
24% identity, 92% coverage: 19:249/251 of query aligns to 24:267/314 of 5exeC
- active site: N143 (≠ M127)
- binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ V50), G51 (= G51), C52 (= C52), I74 (≠ H69), G109 (= G93), D110 (= D94), G111 (= G95), Y136 (≠ V120), N138 (= N122), S140 (≠ I124), Y141 (= Y125), A142 (≠ G126), N143 (≠ M127), T144 (= T128)
- binding magnesium ion: D110 (= D94), D130 (≠ K114), N138 (= N122), S140 (≠ I124), L211 (≠ F195), Q213 (≠ V197)
- binding iron/sulfur cluster: C24 (= C19), C27 (= C22), P29 (≠ H24), C52 (= C52), C225 (= C213), P226 (= P214), K227 (≠ T215)
5c4iC Structure of an oxalate oxidoreductase (see paper)
24% identity, 92% coverage: 19:249/251 of query aligns to 24:267/312 of 5c4iC
- active site: N143 (≠ M127)
- binding magnesium ion: D110 (= D94), N138 (= N122), S140 (≠ I124)
- binding iron/sulfur cluster: C24 (= C19), C27 (= C22), P29 (≠ H24), C52 (= C52), A142 (≠ G126), C225 (= C213), P226 (= P214), K227 (≠ T215)
- binding thiamine diphosphate: T50 (≠ V50), G51 (= G51), C52 (= C52), I74 (≠ H69), G109 (= G93), D110 (= D94), G111 (= G95), Y136 (≠ V120), N138 (= N122), S140 (≠ I124), Y141 (= Y125), A142 (≠ G126), N143 (≠ M127), T144 (= T128)
Query Sequence
>WP_015739363.1 NCBI__GCF_000024605.1:WP_015739363.1
MRVLFERTRGLTSKPFHYCPGCTHGIIHRLVAEVLVELDVLEKTVGVCPVGCAVFAYDYF
NCDMIEAAHGRAPAVATGVKRVLPDRVVFTYQGDGDLAAIGTAEIVHAAARGEKITTIFV
NNAIYGMTGGQMAPTTLLGQKTTTTPFGRRAEEAGYPLRVAEMLATIEGAAYVARVAVNS
PKNVIQAKRVIRRAFEVQLAGLGFSLVEVLSTCPTNWGMSPPEALRWLEEKMIPVYPLGE
FKVPRKEEKHA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory