SitesBLAST

Q96XT2 2-oxoacid:ferredoxin oxidoreductase 2, subunit alpha; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
27% identity, 99% coverage: 3:353/353 of query aligns to 230:618/628 of Q96XT2

query
sites
Q96XT2

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T257 (= T30) binding substrate
R344 (= R106) binding substrate

Q96Y66 2-oxoacid:ferredoxin oxidoreductase 1, subunit alpha; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
37% identity, 53% coverage: 3:190/353 of query aligns to 229:424/627 of Q96Y66

query
sites
Q96Y66

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R

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x
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L

T349 (≠ L111) mutation to L: Same oxidoreductase activity as the wild-type.

Sites not aligning to the query:

41 S→A: Same oxidoreductase activity as the wild-type.
468 D→A: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.

P72578 2-oxoacid:ferredoxin oxidoreductase subunit alpha; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see 2 papers)
37% identity, 53% coverage: 3:190/353 of query aligns to 229:424/632 of P72578

query
sites
P72578

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L

Y253 (= Y27) mutation Y->A,W: Loss of oxidoreductase activity.; mutation to F: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
YPITP 253:257 (= YPITP 27:31) YPITP motif
P254 (= P28) mutation to G: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
I255 (= I29) mutation I->L,M,S,V: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
T256 (= T30) mutation T->A,S,V: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
P257 (= P31) mutation P->A,G,V: Loss of oxidoreductase activity.
R344 (= R106) mutation R->A,K: Loss of oxidoreductase activity.
T353 (≠ I114) Plays a key role in the broad substrate specificity; mutation T->I,V: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.

5b48A 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
37% identity, 49% coverage: 3:174/353 of query aligns to 197:380/576 of 5b48A

query
sites
5b48A

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N

binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: Y221 (= Y27), P222 (= P28), I223 (= I29), E262 (= E56), R312 (= R106)

5exeA Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
27% identity, 97% coverage: 6:349/353 of query aligns to 6:361/394 of 5exeA

query
sites
5exeA

M

I

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S

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I

L

N

E

S

-

L

-

Y

-

I

-

E

-

P

-

E

E

E

A

L

C

E

D

E

E

V

F

N

A

L

R

R

I

L

F

K

G

A

R

K

K

Y

Y

R

D

R

P

M

Y

E

L

E

D

E

E

E

-

K

-

R

-

W

-

E

-

T

-

Y

Y

L

L

L

T

E

D

A

A

E

E

L

V

V

I

L

I

V

F

A

G

F

Q

G

G

T

A

M

H

A

M

R

E

V

T

A

A

R

K

A

A

A

V

V

A

E

R

R

R

L

L

R

R

E

N

E

L

G

G

I

E

A

K

A

V

G

G

L

V

I

A

R

R

P

L

I

R

T

T

L

F

Y

R

P

P

F

F

P

P

D

T

A

E

P

Q

F

I

H

K

A

E

A

R

F

L

S

S

R

K

A

F

R

K

A

A

F

I

-

G

-

V

L

L

V

D

V

V

E

S

M

A

N

N

L

F

G

G

Q

I

M

S

V

C

E

S

D

G

V

G

R

V

L

L

A

L

V

S

E

E

G

L

R

R

C

A

P

A

V

L

H

Y

F

D

Y

Y

G

G

R

D

T

K

G

-

V

-

V

V

P

K

T

T

V

V

A

G

E

F

V

V

V

A

S

G

F

L

A

G

K

G

E

E

V

V

active site: R30 (≠ T30), E58 (= E56), R108 (= R106)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: Y27 (= Y27), P28 (= P28), I29 (= I29), E58 (= E56), V82 (≠ P80), R108 (= R106), F116 (≠ I114)

5exdD Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
27% identity, 97% coverage: 6:349/353 of query aligns to 6:361/394 of 5exdD

query
sites
5exdD

M

I

K

S

G

G

N

C

E

V

A

A

F

V

G

A

E

H

G

G

A

V

I

R

R

L

A

A

G

D

C

V

R

D

Y

V

F

I

F

C

G

S

Y
|
Y

P
|
P

I
|
I

T
x
R

P

P

Q

Y

S

T

E

G

L

I

P

M

H

S

Y

E

L

L

A

A

R

R

R

M

L

V

P

A

-

D

-

G

E

E

V

L

G

D

G

A

V

E

Y

F

L

V

Q

H

A

G

E

E

S

G

E
|
E

V

H

A

A

A

Q

I

L

N

S

M

V

V

V

Y

Y

G

G

A

A

A

S

A

A

G

G

A

A

R

R

V

V

M

F

T

T

S

G

S

S

S

G

P

V

G

G

I

V

S

T

L

Y

M

A

Q

M

E

E

G

V

I

Y

S

S

Y

P

L

I

A

S

G

G

A

E

E

R

L

L

P

P

C

V

V

Q

I

M

V

A

N

I

V

A

M

D

R
|
R

G

T

G

L

P

D

G

P

L

P

G

G

N
x
D

I

F

A

G

P

E

A

E

Q

H

S

T

D

D

Y

A

F

-

Q

E

A

C

V

C

K

R

G

D

G

Q

G

G

H

-

G

-

D

-

Y

-

R

-

L

W

L

I

C

Q

L

G

A

W

P

A

S

S

S

T

V

P

Q

Q

E

E

I

A

L

L

D

D

L

N

M

T

G

L

L

I

A

Y

F

Y

D

R

L

V

A

G

D

E

K

D

Y

Q

R

R

N

V

-

L

P

P

V

Q

M

Y

V

A

L

C

G

L

D

D

G

G

-

Y

-

F

-

V

-

S

-

H

I

I

L

L

G

G

Q

P

M

V

M

D

E

I

P

P

V

D

E

E

-

A

-

Q

-

V

-

K

-

E

-

F

L

L

P

P

Y

Y

S

K

G

N

E

H

L

H

K

V

L

L

P

D

P

P

K

R

P

K

W

P

A

Q

T

I

G

G

-

P

-

Q

-

I

-

E

-

P

-

A

-

M

-

G

-

P

-

L

-

Q

-

Y

-

Q

-

R

-

Y

T

Q

A

A

G

V

R

K

G

G

T

V

R

H

N

K

I

V

I

L

N

E

S

-

L

-

Y

-

I

-

E

-

P

-

E

E

E

A

L

C

E

D

E

E

V

F

N

A

L

R

R

I

L

F

K

G

A

R

K

K

Y

Y

R

D

R

P

M

Y

E

L

E

D

E

E

E

-

K

-

R

-

W

-

E

-

T

-

Y

Y

L

L

L

T

E

D

A

A

E

E

L

V

V

I

L

I

V

F

A

G

F

Q

G

G

T

A

M

H

A

M

R

E

V

T

A

A

R

K

A

A

A

V

V

A

E

R

R

R

L

L

R

R

E

N

E

L

G

G

I

E

A

K

A

V

G

G

L

V

I

A

R

R

P

L

I

R

T

T

L

F

Y

R

P

P

F

F

P

P

D

T

A

E

P

Q

F

I

H

K

A

E

A

R

F

L

S

S

R

K

A

F

R

K

A

A

F

I

-

G

-

V

L

L

V

D

V

V

E

S

M

A

N

N

L

F

G

G

Q

I

M

S

V

C

E

S

D

G

V

G

R

V

L

L

A

L

V

S

E

E

G

L

R

R

C

A

P

A

V

L

H

Y

F

D

Y

Y

G

G

R

D

T

K

G

-

V

-

V

V

P

K

T

T

V

V

A

G

E

F

V

V

V

A

S

G

F

L

A

G

K

G

E

E

V

V

active site: R30 (≠ T30), E58 (= E56), R108 (= R106)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: Y27 (= Y27), P28 (= P28), I29 (= I29), R30 (≠ T30), E58 (= E56), R108 (= R106), D115 (≠ N113)

5c4iA Structure of an oxalate oxidoreductase (see paper)
27% identity, 97% coverage: 6:349/353 of query aligns to 6:361/394 of 5c4iA

query
sites
5c4iA

M

I

K

S

G

G

N

C

E

V

A

A

F

V

G

A

E

H

G

G

A

V

I

R

R

L

A

A

G

D

C

V

R

D

Y

V

F

I

F

C

G

S

Y
|
Y

P

P

I
|
I

T
x
R

P

P

Q

Y

S

T

E

G

L

I

P

M

H

S

Y

E

L

L

A

A

R

R

R

M

L

V

P

A

-

D

-

G

E

E

V

L

G

D

G

A

V

E

Y

F

L

V

Q

H

A

G

E

E

S

G

E
|
E

V

H

A

A

A

Q

I

L

N

S

M

V

V

V

Y

Y

G

G

A

A

A

S

A

A

G

G

A

A

R

R

V

V

M

F

T

T

S

G

S

S

S

G

P
x
V

G

G

I

V

S

T

L

Y

M

A

Q

M

E

E

G

V

I

Y

S

S

Y

P

L

I

A

S

G

G

A

E

E

R

L

L

P

P

C

V

V

Q

I

M

V

A

N

I

V

A

M

D

R
|
R

G

T

G

L

P

D

G

P

L

P

G

G

N

D

I

F

A

G

P

E

A

E

Q

H

S

T

D

D

Y

A

F

-

Q

E

A

C

V

C

K

R

G

D

G

Q

G

G

H

-

G

-

D

-

Y

-

R

-

L

W

L

I

C

Q

L

G

A

W

P

A

S

S

S

T

V

P

Q

Q

E

E

I

A

L

L

D

D

L

N

M

T

G

L

L

I

A

Y

F

Y

D

R

L

V

A

G

D

E

K

D

Y

Q

R

R

N

V

-

L

P

P

V

Q

M

Y

V

A

L

C

G

L

D

D

G

G

-

Y

-

F

-

V

-

S

-

H

I

I

L

L

G

G

Q

P

M

V

M

D

E

I

P

P

V

D

E

E

-

A

-

Q

-

V

-

K

-

E

-

F

L

L

P

P

Y

Y

S

K

G

N

E

H

L

H

K

V

L

L

P

D

P

P

K

R

P

K

W

P

A

Q

T

I

G

G

-

P

-

Q

-

I

-

E

-

P

-

A

-

M

-

G

-

P

-

L

-

Q

-

Y

-

Q

-

R

-

Y

T

Q

A

A

G

V

R

K

G

G

T

V

R

H

N

K

I

V

I

L

N

E

S

-

L

-

Y

-

I

-

E

-

P

-

E

E

E

A

L

C

E

D

E

E

V

F

N

A

L

R

R

I

L

F

K

G

A

R

K

K

Y

Y

R

D

R

P

M

Y

E

L

E

D

E

E

E

-

K

-

R

-

W

-

E

-

T

-

Y

Y

L

L

L

T

E

D

A

A

E

E

L

V

V

I

L

I

V

F

A

G

F

Q

G

G

T

A

M

H

A

M

R

E

V

T

A

A

R

K

A

A

A

V

V

A

E

R

R

R

L

L

R

R

E

N

E

L

G

G

I

E

A

K

A

V

G

G

L

V

I

A

R

R

P

L

I

R

T

T

L

F

Y

R

P

P

F

F

P

P

D

T

A

E

P

Q

F

I

H

K

A

E

A

R

F

L

S

S

R

K

A

F

R

K

A

A

F

I

-

G

-

V

L

L

V

D

V

V

E

S

M

A

N

N

L

F

G

G

Q

I

M

S

V

C

E

S

D

G

V

G

R

V

L

L

A

L

V

S

E

E

G

L

R

R

C

A

P

A

V

L

H

Y

F

D

Y

Y

G

G

R

D

T

K

G

-

V

-

V

V

P

K

T

T

V

V

A

G

E

F

V

V

V

A

S

G

F

L

A

G

K

G

E

E

V

V

active site: R30 (≠ T30), E58 (= E56), R108 (= R106)
binding thiamine diphosphate: Y27 (= Y27), I29 (= I29), E58 (= E56), V82 (≠ P80)

Q2RMD6 Pyruvate:ferredoxin oxidoreductase; PFOR; Pyruvate synthase; EC 1.2.7.1 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
26% identity, 83% coverage: 1:292/353 of query aligns to 1:311/1171 of Q2RMD6

query
sites
Q2RMD6

M

M

P

P

R

K

V

Q

L

T

M

L

K

D

G

G

N

N

E

T

A

A

F

A

G

A

E

H

G

V

A

A

I

Y

R

-

A

A

G

M

C

S

R

E

Y

V

F

A

F

T

G

I

Y

Y

P

P

I

I

T

T

P

P

Q

S

S

S

E

P

L

M

-

A

-

E

-

I

-

A

P

D

H

E

Y

W

L

A

A

A

R

H

R

G

L

R

P

K

E

N

V

I

G

F

G

G

V

K

Y

T

L

L

Q

Q

A

V

-

A

-

E

-

M

E

Q

S

S

E

E

V

A

A

G

A

A

I

A

N

G

M

A

V

V

Y

H

G

G

A

S

A

L

A

A

G

G

A

A

R

L

V

T

M

T

T

T

S

F

S

T

S

A

S

S

P

Q

G

G

I

L

S

L

L

L

M

M

Q

I

E

P

G

N

I

M

S

Y

Y

K

L

I

A

A

G

G

A

E

E

L

L

L

P

P

C

C

V

V

I

F

V

H

N

V

V

A

M

A

R

R

G

A

-

L

G

S

P

T

G

H

L

A

G

L

N

S

I

I

A

F

P

G

A

D

Q

H

S

A

D

D

Y

V

F

M

Q

A

A

A

V

R

K

Q

G

T

G

G

G

-

H

-

G

-

D

-

Y

-

R

-

L

F

L

A

C

M

L

L

A

S

P

S

S

A

S

S

V

V

Q

Q

E

E

I

V

L

M

D

D

L

L

M

A

G

L

L

V

A

A

F

H

D

L

L

A

A

T

D

L

K

K

Y

A

R

R

N

V

P

P

V

F

M

V

V

H

L

F

G

F

D

D

G

G

I

F

-

R

L

T

G

S

Q

H

M

E

M

V

E

Q

P

K

V

I

E

D

L

V

P

I

P

E

Y

Y

S

E

G

D

E

M

L

A

K

K

-

L

-

V

-

D

-

W

-

D

-

A

-

I

-

R

-

A

-

F

-

R

-

Q

-

R

-

A

L

L

P

N

P

P

K

E

P

H

W

P

A

H

T

Q

T

R

G

G

T

T

A

A

G

Q

R

N

-

P

-

D

-

I

-

Y

-

F

G

Q

T

S

R

R

N

E

I

A

N

N

S

P

L

Y

Y

Y

-

L

-

A

-

T

-

P

-

G

I

I

E

V

P

A

E

Q

E

V

L

M

E

E

E

Q

V

V

N

-

L

A

R

G

L

L

K

T

A

G

K

R

Y

H

R

Y

R

H

M

L

E

-

K

-

R

-

W

F

E

D

T

Y

Y

A

L

G

L

A

E

P

E

D

A

A

E

E

L

R

V

V

L

I

V

V

A

S

F

M

G

G

T

S

M

S

A

C

R

E

V

V

A

I

R

E

A

E

A

T

V

V

E

N

R

Y

L

L

R

V

E

E

E

K

G

G

I

E

A

K

A

V

G

G

L

L

I

I

R

K

P

V

I

R

T

L

L

F

Y

R

P

P

F

F

P

S

D

A

A

E

P

H

F

F

Sites not aligning to the query:

424:428 binding CoA
456 binding CoA
556 binding CoA
598 binding CoA
686 binding [4Fe-4S] cluster
689 binding [4Fe-4S] cluster
692 binding [4Fe-4S] cluster
696 binding [4Fe-4S] cluster
742 binding [4Fe-4S] cluster
745 binding [4Fe-4S] cluster
748 binding [4Fe-4S] cluster
752 binding [4Fe-4S] cluster
809 binding [4Fe-4S] cluster
812 binding [4Fe-4S] cluster
814 binding thiamine diphosphate
837 binding [4Fe-4S] cluster; binding thiamine diphosphate
967 binding Mg(2+)
967:969 binding thiamine diphosphate
995 binding Mg(2+)
995:1000 binding thiamine diphosphate
997 binding Mg(2+)
1075 binding [4Fe-4S] cluster

6ciqA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with coenzyme a bound (see paper)
26% identity, 75% coverage: 27:292/353 of query aligns to 25:310/1169 of 6ciqA

query
sites
6ciqA

Y

Y

P
|
P

I
|
I

T
|
T

P

P

Q

S

S

S

E

P

L

M

-

A

-

E

-

I

-

A

P

D

H

E

Y

W

L

A

A

A

R

H

R

G

L

R

P

K

E

N

V

I

G

F

G

G

V

K

Y

T

L

L

Q

Q

A

V

-

A

-

E

-

M

E

Q

S

S

E
|
E

V

A

A

G

A

A

I

A

N

G

M

A

V

V

Y

H

G

G

A

S

A

L

A

A

G

G

A

A

R

L

V

T

M

T

T

T

S

F

S

T

S

A

S

S

P
x
Q

G

G

I

L

S

L

L

L

M

M

Q

I

E

P

G

N

I

M

S

Y

Y

K

L

I

A

A

G

G

A

E

E

L

L

L

P

P

C

C

V

V

I

F

V

H

N

V

V

A

M

A

R
|
R

G

A

-

L

G

S

P

T

G

H

L

A

G

L

N

S

I

I

A

F

P

G

A

D

Q

H

S

A

D

D

Y

V

F

M

Q

A

A

A

V

R

K

Q

G

T

G

G

G

-

H

-

G

-

D

-

Y

-

R

-

L

F

L

A

C

M

L

L

A

S

P

S

S

A

S

S

V

V

Q

Q

E

E

I

V

L

M

D

D

L

L

M

A

G

L

L

V

A

A

F

H

D

L

L

A

A

T

D

L

K

K

Y

A

R

R

N

V

P

P

V

F

M

V

V

H

L

F

G

F

D

D

G

G

I

F

-

R

L

T

G

S

Q

H

M

E

M

V

E

Q

P

K

V

I

E

D

L

V

P

I

P

E

Y

Y

S

E

G

D

E

M

L

A

K

K

-

L

-

V

-

D

-

W

-

D

-

A

-

I

-

R

-

A

-

F

-

R

-

Q

-

R

-

A

L

L

P

N

P

P

K

E

P

H

W

P

A

H

T

Q

T

R

G

G

T

T

A

A

G

Q

R

N

-

P

-

D

-

I

-

Y

-

F

G

Q

T

S

R

R

N

E

I

A

N

N

S

P

L

Y

Y

Y

-

L

-

A

-

T

-

P

-

G

I

I

E

V

P

A

E

Q

E

V

L

M

E

E

E

Q

V

V

N

-

L

A

R

G

L

L

K

T

A

G

K

R

Y

H

R

Y

R

H

M

L

E

-

K

-

R

-

W

F

E

D

T

Y

Y

A

L

G

L

A

E

P

E

D

A

A

E

E

L

R

V

V

L

I

V

V

A

S

F

M

G

G

T

S

M

S

A

C

R

E

V

V

A

I

R

E

A

E

A

T

V

V

E

N

R

Y

L

L

R

V

E

E

E

K

G

G

I

E

A

K

A

V

G

G

L

L

I

I

R

K

P

V

I

R

T

L

L

F

Y

R

P

P

F

F

P

S

D

A

A

E

P

H

F

F

active site: T28 (= T30), E61 (= E56), R111 (= R106)
binding coenzyme a: T28 (= T30)
binding thiamine diphosphate: P26 (= P28), I27 (= I29), E61 (= E56), Q85 (≠ P80)

Sites not aligning to the query:

active site: 999
binding coenzyme a: 423, 424, 427, 428, 553, 586, 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 696, 700, 734, 741, 742, 743, 744, 745, 747, 751, 757, 807, 808, 811, 813, 836, 998, 1074, 1075
binding thiamine diphosphate: 813, 835, 836, 865, 965, 966, 967, 996, 997, 998, 999, 1000

6cinB Crystal structure of pyruvate:ferredoxin oxidoreductase from moorella thermoacetica (see paper)
26% identity, 75% coverage: 27:292/353 of query aligns to 25:310/1169 of 6cinB

query
sites
6cinB

Y

Y

P
|
P

I
|
I

T
|
T

P

P

Q

S

S

S

E

P

L

M

-

A

-

E

-

I

-

A

P

D

H

E

Y

W

L

A

A

A

R

H

R

G

L

R

P

K

E

N

V

I

G

F

G

G

V

K

Y

T

L

L

Q

Q

A

V

-

A

-

E

-

M

E

Q

S

S

E
|
E

V

A

A

G

A

A

I

A

N

G

M

A

V

V

Y

H

G

G

A

S

A

L

A

A

G

G

A

A

R

L

V

T

M

T

T

T

S

F

S

T

S

A

S

S

P

Q

G

G

I

L

S

L

L

L

M

M

Q

I

E

P

G

N

I

M

S

Y

Y

K

L

I

A

A

G

G

A

E

E

L

L

L

P

P

C

C

V

V

I

F

V

H

N

V

V

A

M

A

R
|
R

G

A

-

L

G

S

P

T

G

H

L

A

G

L

N

S

I

I

A

F

P

G

A

D

Q

H

S

A

D

D

Y

V

F

M

Q

A

A

A

V

R

K

Q

G

T

G

G

G

-

H

-

G

-

D

-

Y

-

R

-

L

F

L

A

C

M

L

L

A

S

P

S

S

A

S

S

V

V

Q

Q

E

E

I

V

L

M

D

D

L

L

M

A

G

L

L

V

A

A

F

H

D

L

L

A

A

T

D

L

K

K

Y

A

R

R

N

V

P

P

V

F

M

V

V

H

L

F

G

F

D

D

G

G

I

F

-

R

L

T

G

S

Q

H

M

E

M

V

E

Q

P

K

V

I

E

D

L

V

P

I

P

E

Y

Y

S

E

G

D

E

M

L

A

K

K

-

L

-

V

-

D

-

W

-

D

-

A

-

I

-

R

-

A

-

F

-

R

-

Q

-

R

-

A

L

L

P

N

P

P

K

E

P

H

W

P

A

H

T

Q

T

R

G

G

T

T

A

A

G

Q

R

N

-

P

-

D

-

I

-

Y

-

F

G

Q

T

S

R

R

N

E

I

A

N

N

S

P

L

Y

Y

Y

-

L

-

A

-

T

-

P

-

G

I

I

E

V

P

A

E

Q

E

V

L

M

E

E

E

Q

V

V

N

-

L

A

R

G

L

L

K

T

A

G

K

R

Y

H

R

Y

R

H

M

L

E

-

K

-

R

-

W

F

E

D

T

Y

Y

A

L

G

L

A

E

P

E

D

A

A

E

E

L

R

V

V

L

I

V

V

A

S

F

M

G

G

T

S

M

S

A

C

R

E

V

V

A

I

R

E

A

E

A

T

V

V

E

N

R

Y

L

L

R

V

E

E

E

K

G

G

I

E

A

K

A

V

G

G

L

L

I

I

R

K

P

V

I

R

T

L

L

F

Y

R

P

P

F

F

P

S

D

A

A

E

P

H

F

F

active site: T28 (= T30), E61 (= E56), R111 (= R106)
binding thiamine diphosphate: P26 (= P28), I27 (= I29), E61 (= E56)

Sites not aligning to the query:

active site: 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 455, 678, 685, 686, 687, 688, 689, 691, 695, 700, 741, 742, 743, 744, 745, 746, 747, 751, 752, 757, 758, 807, 808, 811, 813, 836, 998, 1074, 1075
binding thiamine diphosphate: 834, 835, 836, 865, 965, 966, 967, 996, 997, 998, 999, 1000

6cipA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with acetyl-tpp bound (see paper)
26% identity, 75% coverage: 27:292/353 of query aligns to 25:310/1165 of 6cipA

query
sites
6cipA

Y

Y

P

P

I
|
I

T
|
T

P

P

Q

S

S

S

E

P

L

M

-

A

-

E

-

I

-

A

P

D

H

E

Y

W

L

A

A

A

R

H

R

G

L

R

P

K

E

N

V

I

G

F

G

G

V

K

Y

T

L

L

Q

Q

A

V

-

A

-

E

-

M

E

Q

S

S

E
|
E

V

A

A

G

A

A

I

A

N

G

M

A

V

V

Y

H

G

G

A

S

A

L

A

A

G

G

A

A

R

L

V

T

M

T

T

T

S

F

S

T

S

A

S

S

P
x
Q

G

G

I

L

S

L

L

L

M

M

Q

I

E

P

G

N

I

M

S

Y

Y

K

L

I

A

A

G

G

A

E

E

L

L

L

P

P

C

C

V

V

I

F

V

H

N

V

V

A

M

A

R
|
R

G

A

-

L

G

S

P

T

G

H

L

A

G

L

N

S

I

I

A

F

P

G

A

D

Q

H

S

A

D

D

Y

V

F

M

Q

A

A

A

V

R

K

Q

G

T

G

G

G

-

H

-

G

-

D

-

Y

-

R

-

L

F

L

A

C

M

L

L

A

S

P

S

S

A

S

S

V

V

Q

Q

E

E

I

V

L

M

D

D

L

L

M

A

G

L

L

V

A

A

F

H

D

L

L

A

A

T

D

L

K

K

Y

A

R

R

N

V

P

P

V

F

M

V

V

H

L

F

G

F

D

D

G

G

I

F

-

R

L

T

G

S

Q

H

M

E

M

V

E

Q

P

K

V

I

E

D

L

V

P

I

P

E

Y

Y

S

E

G

D

E

M

L

A

K

K

-

L

-

V

-

D

-

W

-

D

-

A

-

I

-

R

-

A

-

F

-

R

-

Q

-

R

-

A

L

L

P

N

P

P

K

E

P

H

W

P

A

H

T

Q

T

R

G

G

T

T

A

A

G

Q

R

N

-

P

-

D

-

I

-

Y

-

F

G

Q

T

S

R

R

N

E

I

A

N

N

S

P

L

Y

Y

Y

-

L

-

A

-

T

-

P

-

G

I

I

E

V

P

A

E

Q

E

V

L

M

E

E

E

Q

V

V

N

-

L

A

R

G

L

L

K

T

A

G

K

R

Y

H

R

Y

R

H

M

L

E

-

K

-

R

-

W

F

E

D

T

Y

Y

A

L

G

L

A

E

P

E

D

A

A

E

E

L

R

V

V

L

I

V

V

A

S

F

M

G

G

T

S

M

S

A

C

R

E

V

V

A

I

R

E

A

E

A

T

V

V

E

N

R

Y

L

L

R

V

E

E

E

K

G

G

I

E

A

K

A

V

G

G

L

L

I

I

R

K

P

V

I

R

T

L

L

F

Y

R

P

P

F

F

P

S

D

A

A

E

P

H

F

F

active site: T28 (= T30), E61 (= E56), R111 (= R106)
binding 2-acetyl-thiamine diphosphate: I27 (= I29), E61 (= E56), Q85 (≠ P80), R111 (= R106)

Sites not aligning to the query:

active site: 999
binding 2-acetyl-thiamine diphosphate: 813, 834, 835, 836, 865, 965, 966, 967, 996, 998, 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836, 998, 1074

6cioA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with lactyl-tpp bound (see paper)
26% identity, 75% coverage: 27:292/353 of query aligns to 25:310/1164 of 6cioA

query
sites
6cioA

Y

Y

P
|
P

I
|
I

T
|
T

P

P

Q

S

S

S

E

P

L

M

-

A

-

E

-

I

-

A

P

D

H

E

Y

W

L

A

A

A

R

H

R

G

L

R

P

K

E

N

V

I

G

F

G

G

V

K

Y

T

L

L

Q

Q

A

V

-

A

-

E

-

M

E

Q

S

S

E
|
E

V

A

A

G

A

A

I

A

N

G

M

A

V

V

Y

H

G

G

A

S

A

L

A

A

G

G

A

A

R

L

V

T

M

T

T

T

S

F

S

T

S

A

S

S

P
x
Q

G

G

I

L

S

L

L

L

M

M

Q

I

E

P

G

N

I

M

S

Y

Y

K

L

I

A

A

G

G

A

E

E

L

L

L

P

P

C

C

V

V

I

F

V

H

N

V

V

A

M

A

R
|
R

G

A

-

L

G

S

P

T

G

H

L

A

G

L

N

S

I

I

A

F

P

G

A

D

Q

H

S

A

D

D

Y

V

F

M

Q

A

A

A

V

R

K

Q

G

T

G

G

G

-

H

-

G

-

D

-

Y

-

R

-

L

F

L

A

C

M

L

L

A

S

P

S

S

A

S

S

V

V

Q

Q

E

E

I

V

L

M

D

D

L

L

M

A

G

L

L

V

A

A

F

H

D

L

L

A

A

T

D

L

K

K

Y

A

R

R

N

V

P

P

V

F

M

V

V

H

L

F

G

F

D

D

G

G

I

F

-

R

L

T

G

S

Q

H

M

E

M

V

E

Q

P

K

V

I

E

D

L

V

P

I

P

E

Y

Y

S

E

G

D

E

M

L

A

K

K

-

L

-

V

-

D

-

W

-

D

-

A

-

I

-

R

-

A

-

F

-

R

-

Q

-

R

-

A

L

L

P

N

P

P

K

E

P

H

W

P

A

H

T

Q

T

R

G

G

T

T

A

A

G

Q

R

N

-

P

-

D

-

I

-

Y

-

F

G

Q

T

S

R

R

N

E

I

A

N

N

S

P

L

Y

Y

Y

-

L

-

A

-

T

-

P

-

G

I

I

E

V

P

A

E

Q

E

V

L

M

E

E

E

Q

V

V

N

-

L

A

R

G

L

L

K

T

A

G

K

R

Y

H

R

Y

R

H

M

L

E

-

K

-

R

-

W

F

E

D

T

Y

Y

A

L

G

L

A

E

P

E

D

A

A

E

E

L

R

V

V

L

I

V

V

A

S

F

M

G

G

T

S

M

S

A

C

R

E

V

V

A

I

R

E

A

E

A

T

V

V

E

N

R

Y

L

L

R

V

E

E

E

K

G

G

I

E

A

K

A

V

G

G

L

L

I

I

R

K

P

V

I

R

T

L

L

F

Y

R

P

P

F

F

P

S

D

A

A

E

P

H

F

F

active site: T28 (= T30), E61 (= E56), R111 (= R106)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: P26 (= P28), I27 (= I29), T28 (= T30), E61 (= E56), Q85 (≠ P80), R111 (= R106)

Sites not aligning to the query:

active site: 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836, 1074, 1075
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 813, 834, 835, 836, 865, 965, 966, 967, 996, 997, 998, 999, 1000

Query Sequence

>WP_015739364.1 NCBI__GCF_000024605.1:WP_015739364.1
MPRVLMKGNEAFGEGAIRAGCRYFFGYPITPQSELPHYLARRLPEVGGVYLQAESEVAAI
NMVYGAAAAGARVMTSSSSPGISLMQEGISYLAGAELPCVIVNVMRGGPGLGNIAPAQSD
YFQAVKGGGHGDYRLLCLAPSSVQEILDLMGLAFDLADKYRNPVMVLGDGILGQMMEPVE
LPPYSGELKLPPKPWATTGTAGRGTRNIINSLYIEPEELEEVNLRLKAKYRRMEEEEKRW
ETYLLEEAELVLVAFGTMARVARAAVERLREEGIAAGLIRPITLYPFPDAPFHAAFSRAR
AFLVVEMNLGQMVEDVRLAVEGRCPVHFYGRTGGVVPTVAEVVSFAKEVWHKS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory