SitesBLAST

K100 (= K66) mutation to A: Abolishes activity on mandelamide.
S180 (= S146) mutation to A: Significantly decreases activity on mandelamide.
S181 (= S147) mutation to A: Significantly decreases activity on mandelamide.
G202 (≠ D168) mutation to A: Increase in KM values for aromatic substrates, but not aliphatic substrates. Active against lactamide but not against mandelamide; when associated with H-207 and E-382.; mutation to V: Increase in KM values for aromatic substrates, but not aliphatic substrates.
S204 (= S170) mutation to A: Abolishes activity on mandelamide.
Q207 (≠ C173) mutation to H: Increases activity on lactamide, does not affect activity on mandelamide; when associated with E-382. Active against lactamide but not against mandelamide; when associated with A-202 and E-382. More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with S-316 and N-437.

Sites not aligning to the query:

31 T→I: More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with N-437.
316 S→N: More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with H-207 and N-437.
382 Q→H: Increases activity on lactamide, does not affect activity on mandelamide; when associated with H-207. Active against lactamide but not against mandelamide; when associated with A-202 and H-207.
437 I→N: More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers. More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with I-31. More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with H-207 and N-316.

3h0mA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
28% identity, 84% coverage: 57:447/466 of query aligns to 63:469/478 of 3h0mA

query
sites
3h0mA

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active site: K72 (= K66), S147 (= S146), S148 (= S147), S166 (≠ T165), T168 (= T167), G169 (≠ D168), G170 (= G169), S171 (= S170), Q174 (≠ C173)
binding glutamine: M122 (≠ N116), G123 (= G121), D167 (≠ E166), T168 (= T167), G169 (≠ D168), G170 (= G169), S171 (= S170), F199 (≠ I198), Y302 (≠ E285), R351 (vs. gap), D418 (= D392)

3h0lA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
28% identity, 84% coverage: 57:447/466 of query aligns to 63:469/478 of 3h0lA

query
sites
3h0lA

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active site: K72 (= K66), S147 (= S146), S148 (= S147), S166 (≠ T165), T168 (= T167), G169 (≠ D168), G170 (= G169), S171 (= S170), Q174 (≠ C173)
binding asparagine: G123 (= G121), S147 (= S146), G169 (≠ D168), G170 (= G169), S171 (= S170), Y302 (≠ E285), R351 (vs. gap), D418 (= D392)

Q9FR37 Amidase 1; AtAMI1; Translocon at the outer membrane of chloroplasts 64-I; AtTOC64-I; EC 3.5.1.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 82% coverage: 58:439/466 of query aligns to 28:417/425 of Q9FR37

query
sites
Q9FR37

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T

T

M

-

D

-

V

V

A

P

W

G

P

P

I

P

D

P

L

H

L

L

R

Q

G

A

D

N

P

V

N

A

S

A

P

L

A

E

G

S

Y

F

R

R

A

S

-

R

-

A

-

F

-

S

-

L

-

S

-

I

A

A

A

G

V

V

A

S

G

G

G

F

T

C

S

Q

V

V

S

S

V

I

P

P

A

L

G

G

R

L

I

H

G

E

H

N

L

L

P

P

V

V

G

S

V

V

C

S

L

L

S

V

A

A

P

K

A

Y

G

G

H

S

E

D

E

G

T

F

I

L

L

L

E

S

L

L

K36 (= K66) active site, Charge relay system; mutation to A: Loss of catalytic activity.; mutation to R: Reduces catalytic activity 10-fold.
S113 (= S146) active site, Charge relay system; mutation S->A,T: Loss of catalytic activity.
S114 (= S147) mutation to A: Loss of catalytic activity.; mutation to T: Reduces catalytic activity 400-fold.
D133 (≠ E166) mutation to A: Loss of catalytic activity.; mutation to E: Reduces catalytic activity 600-fold.
S137 (= S170) active site, Acyl-ester intermediate; mutation to A: Reduces catalytic activity 170-fold.; mutation to T: Loss of catalytic activity.
C145 (≠ N178) mutation C->A,S: Reduces catalytic activity 10-fold.
S214 (= S261) mutation to T: Slightly reduces catalytic activity.

6c6gA An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme. Inhibitor bound complex. (see paper)
43% identity, 34% coverage: 57:215/466 of query aligns to 65:219/457 of 6c6gA

query
sites
6c6gA

P

P

L

L

A

A

G

G

V

L

P

P

V

Y

L

A

V

V

K

K

D

N

T

L

I

F

D

D

T

I

A

E

D

G

L

V

S

T

T

T

T

L

A

A

G

G

S

S

L

K

V

I

L

N

P

R

E

T

E

L

P

P

A

A

-

R

T

A

D

D

A

A

W

V

I

L

V

V

D

Q

A

R

L

L

R

K

A

A

G

G

A

A

T

V

I

L

A

L

A

G

K

G

T

L

T

N

C

M

S

D

E

E

W

F

A

A

-
x
Y

N

G

F

F

R

T

G

T

E

E

G

-

S

-

I

-

S

-

G

-

W

-

N

N

A

T

R

H

Y

Y

G

G

L

P

A

T

R

R

N

N

P

P

Y

H

D

D

P

T

T

G

R

R

S

I

A

A

G

G

S

S

S

G

G

G

S

S

A

G

I

A

A

A

V

I

A

A

G

G

M

Q

S

V

P

P

I

L

A

S

I

L

G

G

T

S

E

D

T

T

D
x
N

G

G

S

S

M

I

L

R

C

V

P

P

A

A

S

S

L

L

N

C

G

G

V

V

I

W

G

G

F

L

K

K

T

P

A

T

P

F

K

G

R

R

L

L

D

S

R

R

S

R

G

G

V

T

I

Y

P

P

I
x
F

S

V

S

H

T

S

Q

I

D

D

S

H

L

L

G

G

I

P

F

L

A

A

T

D

S

S

A

V

D

E

D

G

L

L

binding calcium ion: Y125 (vs. gap), N172 (≠ D168), F202 (≠ I198)

5h6sC Crystal structure of hydrazidase s179a mutant complexed with a substrate (see paper)
41% identity, 29% coverage: 56:190/466 of query aligns to 67:196/457 of 5h6sC

query
sites
5h6sC

G

G

P

P

L

L

A

D

G

G

V

V

P

P

V

L

L

S

V

I

K
|
K

D

D

T

S

I

Y

D

S

T

V

A

A

D

G

L

L

S

H

T

R

T

T

A

D

G

G

S

L

L

P

V

V

L

N

P

A

E

D

E

V

P

L

P

D

A

A

T

Q

D

D

A

D

W

V

I

A

V

T

D

A

A

R

L

L

R

R

A

A

G

G

A

G

T

L

I

V

A

L

A

G

K

H

T

A

-

G

-

I

-

P

-

D

T

L

C
|
C

S

I

E
x
R

W
|
W

A

-

N

-

F

-

R

-

G

-

E

-

G

N

S

S

I

V

S

S

G

G

W

L

N

-

A

-

R

-

Y

Y

G

G

L

A

A

V

R

R

N

N

P

P

Y

R

D

D

P

L

T

S

R

R

S

T

A

A

G

G

S
|
S

S

G

G

G

S

D

A

A

I

A

A

N

V

V

A

A

G

G

M

F

S

A

P

T

I

I

A

G

I

L

G

G

T

G

E

D

T
x
L

D
x
G

G
|
G

S
|
S

M

I

L

R

C

V

P

P

A

A

S

S

L

W

N

C

G

G

V

V

I

Y

G

G

F

F

K

R

T

T

A

G

P

P

K

G

R

R

L

I

active site: K77 (= K66), S152 (= S146), S153 (= S147), L173 (≠ T167), G174 (≠ D168), G175 (= G169), S176 (= S170)
binding 4-oxidanylbenzohydrazide: C126 (= C111), R128 (≠ E113), W129 (= W114), S152 (= S146), L173 (≠ T167), G174 (≠ D168), S176 (= S170)

Sites not aligning to the query:

binding 4-oxidanylbenzohydrazide: 306, 338

Q936X2 Allophanate hydrolase; EC 3.5.1.54 from Pseudomonas sp. (strain ADP) (see paper)
38% identity, 39% coverage: 42:221/466 of query aligns to 64:240/605 of Q936X2

query
sites
Q936X2

A

A

L

L

A

A

A

M

I

L

A

A

D

D

D

A

P

Q

Q

Q

P

R

A

K

A

D

H

K

G

G

-

E

-

A

-

L

P

P

L

L

A

F

G

G

V

I

P

P

V

F

L

G

V

V

K
|
K

D

D

T

N

I

I

D

D

T

V

A

A

D

G

L

L

S

P

T

T

A

A

G

G

S

C

L

T

V

G

L

F

P

A

E

R

E

T

P

-

P

P

A

R

T

Q

D

H

A

A

W

F

I

V

V

V

D

Q

A

R

L

L

R

V

A

D

A

A

G

G

A

A

T

I

I

P

A

I

A

G

K

K

T

T

T

N

C

L

S

D

E

Q

W

F

A

A

N

T

F

-

R

-

G

-

E

-

G

-

S

G

I

L

S

N

G

G

W

T

N

R

A

T

R

P

Y

F

G

G

L

I

A

P

R

R

N

C

P

V

Y

F

D

N

P

E

T

N

R

Y

S

V

A

S

G

G

S
|
S

S

S

G

G

S

S

A

A

I

V

A

A

V

V

A

A

A

N

G

G

M

T

S

V

P

P

I

F

A

S

I

L

G

G

T

T

E

D

T

T

D

A

G

G

S
|
S

M

G

L

R

C

I

P

P

A

A

S

A

L

F

N

N

G

N

V

L

I

V

G

G

F

L

K

K

T

P

A

T

P

K

K

G

R

L

L

F

D

S

R

G

S

S

G

G

V

L

I

V

P

P

I

A

S

A

S

R

T

S

Q

L

D

D

S

C

L

I

G

S

I

V

F

L

A

A

T

H

S

T

A

V

D

D

L

A

L

L

V

A

V

V

A

A

S

R

V

V

K91 (= K66) mutation to A: Loss of activity.
S165 (= S146) mutation to A: Loss of activity.
S189 (= S170) mutation to A: Loss of activity.

1o9oA Crystal structure of the s131a mutant of malonamidase e2 complexed with malonamate from bradyrhizobium japonicum (see paper)
38% identity, 38% coverage: 40:215/466 of query aligns to 36:200/412 of 1o9oA

query
sites
1o9oA

L

V

H

H

A

A

L

F

A

V

A

R

I

H

A

D

D

K

D

S

P

A

Q

R

P

A

A

Q

A

A

H

S

G

G

P

P

L

L

A

R

G

G

V

I

P

A

V

V

L

G

V

I

K
|
K

D

D

T

I

I

I

D

D

T

T

A

A

D

N

L

M

S

P

T

T

T

E

A

M

G

G

S

S

L

E

V

I

L

Y

P

R

E

G

E

W

P

Q

P

P

A

R

T

S

D

D

A

A

W

P

I

V

V

V

D

M

A

M

L

L

R

K

A

R

A

A

G

G

A

A

T

T

I

I

A

I

A

G

K

K

T

T

C

T

S

T

E

A

W

F

A

A

N

S

F

R

R

D

G

P

E

T

G

A

S

T

I

L

S

-

G

-

W

-

N

-

A

-

R

-

Y

-

G

-

L

-

A

-

R

-

N

N

P

P

Y

H

D

N

P

T

T

G

R

H

S

S

A

P

G

G

G
|
G

S
x
A

S
|
S

S

S

G

G

S

S

A

A

I

A

A

A

V

V

A

G

A

A

G

G

M

M

S

I

P

P

I

L

A

A

I

L

G

G

T
|
T

E

Q

T
|
T

D
x
G

G
|
G

S
|
S

M

V

L

I

C
x
R

P

P

A

A

S

A

L

Y

N

C

G

G

V

T

I

A

G

A

F

I

K

K

T

P

A

S

P

F

K

R

R

M

L

L

D

P

R

T

S

V

G

G

V

V

I

K

P

C

I

Y

S

S

S

W

T

A

Q

L

D

D

S

T

L

V

G

G

I

L

F

F

A

G

T

A

S

R

A

A

D

E

D

D

L

L

active site: K62 (= K66), A131 (≠ S146), S132 (= S147), T150 (= T165), T152 (= T167), G153 (≠ D168), G154 (= G169), S155 (= S170), R158 (≠ C173)
binding 3-amino-3-oxopropanoic acid: G130 (= G145), T152 (= T167), G153 (≠ D168), G154 (= G169), S155 (= S170), R158 (≠ C173)

Sites not aligning to the query:

binding 3-amino-3-oxopropanoic acid: 359

1ocmA The crystal structure of malonamidase e2 covalently complexed with pyrophosphate from bradyrhizobium japonicum (see paper)
39% identity, 38% coverage: 40:215/466 of query aligns to 36:200/412 of 1ocmA

query
sites
1ocmA

L

V

H

H

A

A

L

F

A

V

A

R

I

H

A

D

D

K

D

S

P

A

Q

R

P

A

A

Q

A

A

H

S

G

G

P

P

L

L

A

R

G

G

V

I

P

A

V

V

L

G

V

I

K
|
K

D

D

T

I

I

I

D

D

T

T

A

A

D

N

L

M

S

P

T

T

T

E

A

M

G

G

S

S

L

E

V

I

L

Y

P

R

E

G

E

W

P

Q

P

P

A

R

T

S

D

D

A

A

W

P

I

V

V

V

D

M

A

M

L

L

R

K

A

R

A

A

G

G

A

A

T

T

I

I

A

I

A

G

K

K

T

T

C

T

S

T

E

A

W

F

A

A

N

S

F
x
R

R

D

G

P

E

T

G

A

S

T

I

L

S

-

G

-

W

-

N

-

A

-

R

-

Y

-

G

-

L

-

A

-

R

-

N

N

P

P

Y

H

D

N

P

T

T

G

R

H

S

S

A

P

G

G

S
|
S

S

S

G

G

S

S

A

A

I

A

A

A

V

V

A

G

A

A

G

G

M

M

S

I

P

P

I

L

A

A

I

L

G

G

T

T

E
x
Q

T
|
T

D
x
G

G
|
G

S
|
S

M

V

L

I

C
x
R

P

P

A

A

S

A

L

Y

N

C

G

G

V

T

I

A

G

A

F

I

K

K

T

P

A

S

P

F

K

R

R

M

L

L

D

P

R

T

S

V

G

G

V

V

I

K

P

C

I

Y

S

S

S

W

T

A

Q

L

D

D

S

T

L

V

G

G

I

L

F

F

A

G

T

A

S

R

A

A

D

E

D

D

L

L

active site: K62 (= K66), S131 (= S146), S132 (= S147), T152 (= T167), G153 (≠ D168), G154 (= G169), S155 (= S170)
binding pyrophosphate 2-: R113 (≠ F117), S131 (= S146), Q151 (≠ E166), T152 (= T167), G153 (≠ D168), G154 (= G169), S155 (= S170), R158 (≠ C173)

Sites not aligning to the query:

binding pyrophosphate 2-: 359

4n0iA Crystal structure of s. Cerevisiae mitochondrial gatfab in complex with glutamine (see paper)
32% identity, 48% coverage: 51:273/466 of query aligns to 23:262/450 of 4n0iA

query
sites
4n0iA

Q

N

P

P

A

S

A

P

H

Y

G

S

P

T

L

L

A

T

G

G

V

C

P

V

V

A

L

S

V

I

K
|
K

D

D

T

N

I

I

D

V

T

T

A

K

D

D

L

F

S

P

T

T

A

C

G

A

S

S

L

H

V

I

L

L

P

E

E

N

E

F

P

K

P

S

A

P

T

F

D

D

A

A

W

T

I

V

V

V

D

K

A

L

L

L

R

K

A

Q

A

A

G

G

A

V

T

H

I

I

A

L

A

G

K

K

T

T

T

N

C

L

S

D

E

E

W

F

A

-

N

-

F

-

R

-

G

G

E

M

G
|
G

S

S

I

-

S

G

G

G

W

V

N

H

A

S

R

I

Y

R

G

G

L

P

A

V

R

I

N

N

P

P

Y

L

D

Y

P

P

-

H

-

E

-

D

T

K

R

K

S

I

A

M

G

G

S
|
S

S

S

G

G

S

A

A

A

I

A

A

S

V

V

A

A

A

C

G

D

M

L

S

V

P

D

I

F

A

A

I

L

G

G

T
|
T

E

D

T
|
T

D
x
G

G
|
G

S
|
S

M

V

L

R

C
x
L

P

P

A

A

S

C

L

Y

N

G

G

S

V

V

I

L

G

G

F

F

K

K

T

P

A

S

P

Y

K

G

R

R

L

L

D

S

R

R

S

F

G

G

V

V

I

I

P

A

I
x
Y

S

S

S

Q

T

S

Q

L

D

D

S

T

L

V

G

G

I

I

F

L

A

S

T

K

S

K

A

I

D

N

D

V

L

L

L

R

V

K

V

V

A

F

-

H

-

T

-

L

-

D

-

K

-

Y

-

D

-

M

-

K

-

D

-

P

S

T

V

S

L

L

G

S

I

V

E

E

A

L

T

R

T

E

L

L

T

I

E

E

-

G

-

N

-

K

-

V

-

R

P

P

V

L

R

K

L

V

C

G

V

I

V

V

E

K

E

E

S

F

L

S

N

H

G

E

F

S

H

M

P

P

R

I

T

G

L

F

A

H

R

R

F

L

H

Y

-

L

D

S

V

L

L

L

E

E

L

K

L

L

S

I

R

N

A

L

G

G

V

L

D

E

V

I

V

Y

T

P

L

V

S

S

A

I

P

P

active site: K38 (= K66), S116 (= S146), S117 (= S147), T135 (= T165), T137 (= T167), G138 (≠ D168), G139 (= G169), S140 (= S170), L143 (≠ C173)
binding glutamine: G89 (= G121), T137 (= T167), G138 (≠ D168), S140 (= S170), Y168 (≠ I198)

Sites not aligning to the query:

binding glutamine: 271, 272, 320, 404

4gysB Granulibacter bethesdensis allophanate hydrolase co-crystallized with malonate (see paper)
30% identity, 89% coverage: 49:465/466 of query aligns to 55:459/461 of 4gysB

query
sites
4gysB

D

D

P

A

Q

S

P

P

A

A

A

T

H

G

G

K

P

P

L

L

A

Y

G

G

V

V

P

P

V

F

L

A

V

V

K
|
K

D

D

T

N

I

I

D

D

T

V

A

A

D

G

L

L

S

P

T

C

T

S

A

A

G

A

S

C

L

P

V

A

L

F

P

T

E

Y

E

E

P

P

P

D

A

-

T

R

D

D

A

A

W

T

I

V

V

V

D

A

A

R

L

L

R

R

A

A

G

G

A

A

T

I

I

V

A

L

A

G

K

K

T

T

T

N

C

L

S

D

E

Q

W

F

A
|
A

N

T

F

-

R

-

G

-

E

-

G

-

S
x
G

I

L

S

V

G

G

W

T

N

R

A

S

R

P

Y

F

G

G

L

A

A

P

R

R

N

C

P

V

Y

F

D

D

P

Q

T

D

R

Y

S

I

A

S

G

G

S
|
S

S

S

G

G

S

S

A

A

I

V

A

A

V

V

A

A

G

G

M

L

S

V

P

A

I

F

A

S

I

L

G

G

T
|
T

E

D

T
|
T

D
x
A

G
|
G

S
|
S

M

G

L

R

C
x
V

P

P

A

A

S

A

L

F

N

N

G

N

V

L

I

V

G

G

F

V

K

K

T

P

A

T

P

K

K

G

R

L

L

L

D

S

R

T

S
|
S

G
|
G

V
|
V

I

V

P

P

I

A

S

C

S
x
R

T

S

Q

L

D

D

S

C

L

V

G

T

I

V

F

F

A

A

T

A

S

S

A

V

D

A

D

E

L

G

L

T

V

L

V

I

A

R

S

R

V

I

L

-

G

-

I

A

E

E

A

G

T

Y

T

D

L

A

T

A

E

D

P

P

V

Y

R

S

L

R

C

P

V

S

V

Q

E

K

E

R

S

R

L

L

N

P

G

H

F

V

H

G

P

L

R

R

T

V

L

G

A

V

R

P

F

R

H

Q

D

D

V

Q

L

R

E

E

L

F

L

Y

S

G

R

N

A

T

G

A

V

Y

D

-

V

-

T

-

L

-

S

-

A

-

P

-

R

-

G

A

T

A

L

L

E

Y

P

Q

S

R

D

A

E

L

D

D

E

E

L

M

I

I

V

S

L

L

R

D

T

A

E

E

L

L

H

-

V

V

E

E

L

I

D

D

R

-

Y

-

L

-

D

-

R

-

A

-

I

F

P

A

G

P

L

F

R

R

S

D

L

A

A

A

D

K

V

L

A
x
Y

A

G

N

G

D

P

A

W

L

V

A

A

D

E

R
|
R

E

-

L

L

A

E

Y

A

F

V

G

G

Q

-

E

D

H

H

F

L

V

S

A

R

A

A

L

-

E

-

A

P

D

D

P

S

A

F

D

D

P

P

A

V

Y

V

R

R

A

S

A

I

R

V

-

E

-

T

-

A

-

K

-

T

-

L

-

S

-

A

-

V

-

D

-

A

-

F

-

R

-

G

Q

Q

R

Y

N

E

L

L

E

A

R

A

T

L

T

T

E

Q

G

Q

I

A

E

N

S

A

S

Q

L

W

A

A

A

R

T

M

D

D

A

I

L

L

A

-

I

-

L

L

A

L

P

P

T

T

M

A

D

P

V

T

A

I

W

H

P

K

I

V

D

E

L

A

L

V

R

M

G

A

D

D

P

P

-

V

-

R

-

L

N

N

S

S

P

Q

A

L

G

G

Y

H

R

Y

A

T

A

N

A

F

V

V

A

N

-

L

G

D

G

C

T

A

S

A

V

I

S

A

V

V

P

P

A

A

G

G

R

F

I

I

-

E

G

T

H

G

L

L

P

P

V

F

G

G

V

V

C

T

L

L

S

V

A

G

P

P

A

A

G

F

H

S

E

D

T

S

I

M

L

A

E

L

L

I

T

A

A

D

L

R

L

L

E

H

R

R

A

R

L

L

A

E

P

P

L

G

A

Y

G

G

L

Q

A

D

G

R

R

A

E

S

R

L

P

P

T

D

T

P

L

V

L

L

P

E

E

E

active site: K72 (= K66), S146 (= S146), S147 (= S147), T165 (= T165), T167 (= T167), A168 (≠ D168), G169 (= G169), S170 (= S170), V173 (≠ C173)
binding malonate ion: A120 (= A115), G122 (≠ S122), S146 (= S146), T167 (= T167), A168 (≠ D168), S170 (= S170), S193 (= S193), G194 (= G194), V195 (= V195), R200 (≠ S200), Y297 (≠ A317), R305 (= R325)

3a1iA Crystal structure of rhodococcus sp. N-771 amidase complexed with benzamide (see paper)
34% identity, 39% coverage: 41:222/466 of query aligns to 60:246/508 of 3a1iA

query
sites
3a1iA

H

H

A

A

L

V

A

P

A

S

I

A

A

S

D

E

D

N

P

P

-

L

-

S

-

A

-

W

-

Y

-

V

-

T

-

S

-

I

Q

P

P

P

A

T

A

S

H

D

G

G

P

V

L

L

A

T

G

G

V

R

P

R

V

V

L

A

V

I

K
|
K

D

D

T

N

I

V

D

T

T

V

A

A

D

G

L

V

S

P

T

M

A

N

G

G

S

S

L

R

V

T

L

V

P

E

E

G

E

F

P

T

P

P

A

S

T

R

D

D

A

A

W

T

I

V

V

V

D

T

A

R

L

L

R

L

A

A

G

G

A

A

T

T

I

V

A

A

A

G

K

K

T

A

T

V

C

C

S

E

E

D

W

L

A

C

N

-

F
|
F

R
x
S

G
|
G

E

-

G

-

S

-

I

-

S

S

G

S

W

F

N

T

A

P

R

A

Y

S

G

G

L

P

A

V

R

R

N

N

P

P

Y

W

D

D

P

R

T

Q

R

R

S

E

A

A

G

G

S
|
S

S

G

G

G

S

S

A

A

I

A

A

L

V

V

A

A

A

N

G

G

M

D

S

V

P

D

I

F

A

A

I

I

G

G

T
x
G

E

D

T
x
Q

D
x
G

G
|
G

S
x
A

M

I

L

R

C
x
I

P

P

A

A

S

A

L

F

N

C

G

G

V

V

I

V

G

G

F

H

K

K

T

P

A

T

P

F

K

G

R

L

L

V

D

P

R

Y

S

T

G

G

V

A

I

F

P

P

I

I

S

E

S

R

T

T

Q

I

D

D

S

H

L

L

G

G

I

P

F

I

A

T

T

R

S

T

A

V

D

H

D

D

L

A

V

L

V

M

A

L

S

S

V

V

L

I

active site: K95 (= K66), S170 (= S146), S171 (= S147), G189 (≠ T165), Q191 (≠ T167), G192 (≠ D168), G193 (= G169), A194 (≠ S170), I197 (≠ C173)
binding benzamide: F145 (= F117), S146 (≠ R118), G147 (= G119), Q191 (≠ T167), G192 (≠ D168), G193 (= G169), A194 (≠ S170)

Sites not aligning to the query:

binding benzamide: 327

2f2aA Structure of tRNA-dependent amidotransferase gatcab complexed with gln (see paper)
33% identity, 35% coverage: 56:218/466 of query aligns to 69:226/485 of 2f2aA

query
sites
2f2aA

G

G

P

K

L

L

A

F

G

G

V

I

P

P

V

M

L

G

V

I

K
|
K

D

D

T

N

I

I

D

I

T

T

A

N

D

G

L

L

S

E

T

T

A

C

G

A

S

S

L

K

V

M

L

L

P

E

E

G

E

F

P

V

P

P

A

I

T

Y

D

E

A

S

W

T

I

V

V

M

D

E

A

K

L

L

R

H

A

K

A

E

G

N

A

A

T

V

I

L

A

I

A

G

K

K

T

L

T

N

C

M

S

D

E

E

W

F

A

A

N

-

F

-

R

M

G
|
G

E

G

G

S

S

T

I

E

S

T

G

S

W

Y

N

F

A

K

R

K

Y

-

G

-

L

-

A

T

R

V

N

N

P

P

Y

F

D

D

P

H

T

K

R

A

S

V

A

P

G

G

S
|
S

S

G

G

G

S

S

A

A

I

A

A

A

V

V

A

A

G

G

M

L

S

V

P

P

I

L

A

S

I

L

G

G

T
x
S

E
x
D

T
|
T

D
x
G

G
|
G

S
|
S

M

I

L

R

C
x
Q

P

P

A

A

S

A

L

Y

N

C

G

G

V

V

I

V

G

G

F

M

K

K

T

P

A

T

P

Y

K

G

R

R

L

V

D

S

R

R

S

F

G

G

V

L

I

V

P

A

I
x
F

S

A

S

S

T

S

Q

L

D

D

S

Q

L

I

G

G

I

P

F

L

A

T

T

R

S

N

A

V

D

K

D

D

L

N

L

A

V

I

V

V

active site: K79 (= K66), S154 (= S146), S155 (= S147), S173 (≠ T165), T175 (= T167), G176 (≠ D168), G177 (= G169), S178 (= S170), Q181 (≠ C173)
binding glutamine: G130 (= G119), S154 (= S146), D174 (≠ E166), T175 (= T167), G176 (≠ D168), S178 (= S170), F206 (≠ I198)

Sites not aligning to the query:

binding glutamine: 309, 310, 358, 425

2dqnA Structure of tRNA-dependent amidotransferase gatcab complexed with asn (see paper)
33% identity, 35% coverage: 56:218/466 of query aligns to 69:226/485 of 2dqnA

query
sites
2dqnA

G

G

P

K

L

L

A

F

G

G

V

I

P

P

V

M

L

G

V

I

K
|
K

D

D

T

N

I

I

D

I

T

T

A

N

D

G

L

L

S

E

T

T

A

C

G

A

S

S

L

K

V

M

L

L

P

E

E

G

E

F

P

V

P

P

A

I

T

Y

D

E

A

S

W

T

I

V

V

M

D

E

A

K

L

L

R

H

A

K

A

E

G

N

A

A

T

V

I

L

A

I

A

G

K

K

T

L

T

N

C

M

S

D

E

E

W

F

A

A

N

-

F

-

R
x
M

G
|
G

E

G

G

S

S

T

I

E

S

T

G

S

W

Y

N

F

A

K

R

K

Y

-

G

-

L

-

A

T

R

V

N

N

P

P

Y

F

D

D

P

H

T

K

R

A

S

V

A

P

G

G

S
|
S

S

G

G

G

S

S

A

A

I

A

A

A

V

V

A

A

G

G

M

L

S

V

P

P

I

L

A

S

I

L

G

G

T
x
S

E

D

T
|
T

D
x
G

G
|
G

S
|
S

M

I

L

R

C
x
Q

P

P

A

A

S

A

L

Y

N

C

G

G

V

V

I

V

G

G

F

M

K

K

T

P

A

T

P

Y

K

G

R

R

L

V

D

S

R

R

S

F

G

G

V

L

I

V

P

A

I

F

S

A

S

S

T

S

Q

L

D

D

S

Q

L

I

G

G

I

P

F

L

A

T

T

R

S

N

A

V

D

K

D

D

L

N

L

A

V

I

V

V

active site: K79 (= K66), S154 (= S146), S155 (= S147), S173 (≠ T165), T175 (= T167), G176 (≠ D168), G177 (= G169), S178 (= S170), Q181 (≠ C173)
binding asparagine: M129 (≠ R118), G130 (= G119), T175 (= T167), G176 (≠ D168), S178 (= S170)

Sites not aligning to the query:

binding asparagine: 309, 310, 358, 425

6te4A Structural insights into pseudomonas aeruginosa type six secretion system exported effector 8: tse8 in complex with a peptide (see paper)
32% identity, 36% coverage: 56:222/466 of query aligns to 72:236/564 of 6te4A

query
sites
6te4A

G

G

P

P

L

L

A

D

G

G

V

I

P

P

V

Y

L

T

V

A

K

K

D

D

T

S

I

Y

D

L

T

V

A

K

D

G

L

L

S

T

T

A

A

S

G

G

S

S

L

P

V

A

L

F

P

K

E

D

E

L

P

V

P

A

A

Q

T

R

D

D

A

A

W

F

I

T

V

V

D

E

A

R

L

L

R

R

A

A

G

G

A

A

T

I

I

C

A

L

A

G

K

K

T

T

T

N

C

M

S

P

E

P

W

M

A

A

N
|
N

F
x
G

R

G

G

M

E

Q

G

R

S

G

I

V

S

-

G

-

W

-

N

-

A

-

R

-

Y

Y

G

G

L

R

A

A

R

E

N

S

P

P

Y

Y

D

N

P

A

T

A

R

Y

-

L

-

T

-

A

-

P

S

F

A

A

G

S

G

G

S

S

S

N

G

G

S

A

A

G

I

T

A

A

V

T

A

A

G

S

M

F

S

A

P

A

I

F

A

G

I

L

G

A

T

E

E

E

T

T

D
x
W

G

S

S

S

M

G

L

R

C

G

P

P

A

A

S

S

L

N

N

N

G

G

V

L

I

C

G

A

F

Y

K

T

T

P

A

S

P

R

K

G

R

V

L

I

D

S

R

V

S

R

G

G

V

N

I

W

P

P

I
x
L

S
x
T

S

P

T

T

Q
x
M

D

D

S

V

L

V

G

V

I

P

F

Y

A

A

T

R

S

S

A

M

D

A

D

D

L

L

V

E

V

I

A

L

S

D

V

V

L

V

binding : N132 (= N116), G133 (≠ F117), W182 (≠ D168), L212 (≠ I198), T213 (≠ S199), M216 (≠ Q202)

Sites not aligning to the query:

binding : 367, 371, 375, 414

3a2qA Structure of 6-aminohexanoate cyclic dimer hydrolase complexed with substrate (see paper)
34% identity, 36% coverage: 54:222/466 of query aligns to 57:226/482 of 3a2qA

query
sites
3a2qA

A

A

H

S

G

G

P

P

L

F

A

A

G

G

V

V

P

P

V

Y

L

L

V

L

K
|
K

D

D

-

L

-

T

T

V

I

V

D

S

T

Q

A

G

D

D

L

I

S

N

T

T

T

S

A

S

G

I

S

K

L

G

V

M

L

K

P

E

E

S

E

G

P

Y

P

R

A

A

T

D

-

H

D

D

A

A

W

Y

I

F

V

V

D

Q

A

R

L

M

R

R

A

A

G

G

A

F

T

V

I

L

A

L

A

G

K

K

T

T

T

N

C

T

S

P

E

E

W

M

A
x
G

N
|
N

F

-

R

-

G

-

E

-

G

-

S

Q

I

V

S

T

G

T

W

E

N

P

A

E

R

A

Y

W

G

G

L

A

A

T

R

R

N

N

P

P

Y

W

D

N

P

L

T

G

R

R

S

S

A

V

G

G

S
|
S

S

G

G

G

S

S

A

G

I

A

A

A

V

V

A

A

G

A

M

L

S

S

P

P

I

V

A

A

I

H

G

G

T
x
N

E

D

T
x
A

D
x
A

G
|
G

S
x
A

M

V

L

R

C
x
I

P

P

A

A

S

S

L

V

N

C

G

G

V

V

I

V

G

G

F

L

K

K

T

P

A

T

P

R

K

G

R

R

L

I

D

S

R

P

S

G

G

-

V

-

I

-

P

P

I

L

S

V

S

T

T

D

Q

S

D

D

S

N

L

V

-

A

-

G

-

A

-

H

-

E

G

G

I

L

F

F

A

A

T

R

S

S

A

V

D

R

D

D

L

I

L

A

V

A

V

L

A

L

S

D

V

V

L

V

active site: K69 (= K66), S147 (= S146), S148 (= S147), N166 (≠ T165), A168 (≠ T167), A169 (≠ D168), G170 (= G169), A171 (≠ S170), I174 (≠ C173)
binding 6-aminohexanoic acid: G121 (≠ A115), G121 (≠ A115), N122 (= N116), S147 (= S146), A168 (≠ T167), A168 (≠ T167), A169 (≠ D168), A171 (≠ S170)

Sites not aligning to the query:

binding 6-aminohexanoic acid: 313

Query Sequence

>WP_015798864.1 NCBI__GCF_000023265.1:WP_015798864.1
MTDVLSPFTPVTIDRTRTEFPGIGAAWRAHALRIDDRLGLHALAAIADDPQPAAHGPLAG
VPVLVKDTIDTADLSTTAGSLVLPEEPPATDAWIVDALRAAGATIAAKTTCSEWANFRGE
GSISGWNARYGLARNPYDPTRSAGGSSSGSAIAVAAGMSPIAIGTETDGSMLCPASLNGV
IGFKTAPKRLDRSGVIPISSTQDSLGIFATSADDLLVVASVLGIEATTLTEPVRLCVVEE
SLNGFHPRTLARFHDVLELLSRAGVDVVTLSAPPRGTLEPSDEDELIVLRTELHVELDRY
LDRRAIPGLRSLADVVAANDALADRELAYFGQEHFVAALEADPADPAYRAARQRNLERTT
EGIESSLAATDALAILAPTMDVAWPIDLLRGDPNSPAGYRAAAVAGGTSVSVPAGRIGHL
PVGVCLSAPAGHEETILELTALLERALAPLAGLAGRERPTTLLPEA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory