SitesBLAST
Comparing WP_015798930.1 NCBI__GCF_000023265.1:WP_015798930.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
40% identity, 96% coverage: 5:453/468 of query aligns to 4:458/468 of 8i9iA
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
40% identity, 96% coverage: 5:453/468 of query aligns to 4:458/471 of P04805
- C98 (= C99) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (= C101) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (= C122) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (= H124) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ G126) mutation to Q: No change in activity or in zinc content.
- H131 (≠ G128) mutation to Q: No change in activity or in zinc content.
- H132 (≠ P129) mutation to Q: No change in activity or in zinc content.
- C138 (vs. gap) mutation to S: No change in activity or in zinc content.
- S239 (= S233) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
36% identity, 96% coverage: 5:452/468 of query aligns to 3:475/485 of 4griB
- active site: S9 (= S11), K253 (= K234)
- binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (≠ D43), Y194 (= Y175), R212 (= R193), W216 (≠ H197)
- binding zinc ion: C105 (= C99), C107 (= C101), Y128 (= Y118), C132 (= C122)
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
39% identity, 94% coverage: 5:443/468 of query aligns to 4:464/485 of Q8DLI5
- R6 (= R7) binding L-glutamate
- Y192 (= Y175) binding L-glutamate
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
39% identity, 94% coverage: 5:443/468 of query aligns to 3:463/484 of 2cfoA
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
36% identity, 96% coverage: 5:452/468 of query aligns to 104:555/564 of 3al0C
- active site: S110 (= S11), K335 (= K234)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R7), A108 (= A9), P109 (= P10), G118 (= G19), T122 (= T23), E142 (≠ D43), Y276 (= Y175), R294 (= R193), G295 (≠ A194), D297 (≠ E196), H298 (= H197), L324 (≠ F223), I325 (≠ L224), L333 (= L232)
- binding : T144 (= T45), D145 (= D46), R148 (= R49), Y208 (vs. gap), P213 (≠ C101), K252 (≠ R151), M255 (≠ V154), I266 (≠ V165), K269 (= K168), S270 (= S169), Y276 (= Y175), D297 (≠ E196), H298 (= H197), L299 (= L198), S300 (≠ P199), N301 (≠ T200), K304 (= K203), R330 (= R229), P332 (≠ K231), G363 (= G262), W364 (= W263), R365 (≠ S264), E370 (= E270), S387 (≠ Q287), K389 (≠ S289), V391 (≠ A291), I392 (≠ F292), K397 (= K297), W400 (≠ A300), R407 (≠ Q307), E446 (≠ P346), K447 (≠ R347), Q453 (≠ E353), I457 (≠ A356), R509 (= R406), K520 (≠ R417), Q524 (≠ A421), R527 (= R424), V535 (= V432), T536 (≠ G433), G538 (≠ P435), L539 (= L436)
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
35% identity, 97% coverage: 1:452/468 of query aligns to 1:462/488 of 8vc5A
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
41% identity, 79% coverage: 3:370/468 of query aligns to 2:361/380 of 4g6zA
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
39% identity, 96% coverage: 6:453/468 of query aligns to 4:464/468 of 1g59A
- binding : D44 (= D46), R45 (≠ E47), A46 (≠ E48), R47 (= R49), P109 (≠ R103), V145 (≠ A134), R163 (= R151), V166 (= V154), E172 (≠ S160), V177 (= V165), K180 (= K168), S181 (= S169), D182 (≠ T170), E207 (= E195), E208 (= E196), R237 (≠ V225), K241 (≠ R229), T242 (≠ R230), K243 (= K231), M273 (≠ L261), G274 (= G262), E282 (= E270), S299 (≠ Q287), L300 (≠ H288), P303 (≠ A291), V304 (≠ F292), K309 (= K297), W312 (≠ A300), R319 (vs. gap), P357 (= P346), R358 (= R347), R417 (= R406), K426 (≠ S415), L427 (= L416), Q432 (≠ A421), R435 (= R424), L442 (≠ R431), E443 (≠ V432), T444 (≠ G433), P445 (= P434), G446 (≠ P435), L447 (= L436), F448 (= F437)
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
39% identity, 96% coverage: 6:453/468 of query aligns to 4:464/468 of 2cv2A
- active site: K246 (= K234)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R7), A7 (= A9), S9 (= S11), G17 (= G19), I21 (≠ T23), E41 (≠ D43), Y187 (= Y175), R205 (= R193), A206 (= A194), E208 (= E196), W209 (≠ H197), L235 (≠ F223), L236 (= L224)
- binding : S9 (= S11), T43 (= T45), D44 (= D46), R47 (= R49), V145 (≠ A134), R163 (= R151), Y168 (≠ F156), E172 (≠ S160), V177 (= V165), K180 (= K168), S181 (= S169), Y187 (= Y175), E207 (= E195), E208 (= E196), W209 (≠ H197), V211 (≠ P199), R237 (≠ V225), K241 (≠ R229), L272 (= L260), M273 (≠ L261), G274 (= G262), E282 (= E270), S299 (≠ Q287), P303 (≠ A291), V304 (≠ F292), K309 (= K297), W312 (≠ A300), R319 (vs. gap), P357 (= P346), R358 (= R347), R417 (= R406), Q432 (≠ A421), R435 (= R424), L442 (≠ R431), E443 (≠ V432), T444 (≠ G433), G446 (≠ P435), L447 (= L436), F448 (= F437)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
39% identity, 96% coverage: 6:453/468 of query aligns to 4:464/468 of 2cv1A
- active site: K246 (= K234)
- binding adenosine-5'-triphosphate: P8 (= P10), S9 (= S11), G17 (= G19), T18 (≠ S20), I21 (≠ T23), R47 (= R49), A206 (= A194), W209 (≠ H197), L235 (≠ F223), L236 (= L224)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R7), A7 (= A9), E41 (≠ D43), Y187 (= Y175), R205 (= R193), W209 (≠ H197)
- binding : S9 (= S11), E41 (≠ D43), T43 (= T45), D44 (= D46), R47 (= R49), V145 (≠ A134), R163 (= R151), V166 (= V154), E172 (≠ S160), V177 (= V165), K180 (= K168), S181 (= S169), Y187 (= Y175), E207 (= E195), E208 (= E196), W209 (≠ H197), V211 (≠ P199), R237 (≠ V225), K241 (≠ R229), K243 (= K231), M273 (≠ L261), G274 (= G262), S276 (= S264), E282 (= E270), S299 (≠ Q287), P303 (≠ A291), V304 (≠ F292), K309 (= K297), W312 (≠ A300), R319 (vs. gap), P357 (= P346), R358 (= R347), R417 (= R406), L427 (= L416), Q432 (≠ A421), R435 (= R424), L442 (≠ R431), E443 (≠ V432), T444 (≠ G433), G446 (≠ P435), L447 (= L436), F448 (= F437)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
39% identity, 96% coverage: 6:453/468 of query aligns to 4:464/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
39% identity, 96% coverage: 6:453/468 of query aligns to 4:464/468 of 1n78A
- active site: K246 (= K234)
- binding glutamol-amp: R5 (= R7), A7 (= A9), P8 (= P10), S9 (= S11), G17 (= G19), T18 (≠ S20), I21 (≠ T23), E41 (≠ D43), Y187 (= Y175), N191 (= N179), R205 (= R193), A206 (= A194), E208 (= E196), W209 (≠ H197), L235 (≠ F223), L236 (= L224)
- binding : S9 (= S11), T43 (= T45), D44 (= D46), R47 (= R49), V145 (≠ A134), R163 (= R151), V166 (= V154), Y168 (≠ F156), E172 (≠ S160), V177 (= V165), K180 (= K168), S181 (= S169), Y187 (= Y175), E207 (= E195), E208 (= E196), W209 (≠ H197), L210 (= L198), V211 (≠ P199), R237 (≠ V225), K241 (≠ R229), M273 (≠ L261), G274 (= G262), E282 (= E270), R297 (= R285), P303 (≠ A291), V304 (≠ F292), K309 (= K297), W312 (≠ A300), R319 (vs. gap), P357 (= P346), R358 (= R347), R417 (= R406), L427 (= L416), Q432 (≠ A421), R435 (= R424), L442 (≠ R431), E443 (≠ V432), T444 (≠ G433), G446 (≠ P435), L447 (= L436), F448 (= F437)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
39% identity, 96% coverage: 6:453/468 of query aligns to 4:464/468 of 1j09A
- active site: K246 (= K234)
- binding adenosine-5'-triphosphate: H15 (= H17), E208 (= E196), L235 (≠ F223), L236 (= L224), K243 (= K231), I244 (≠ L232), S245 (= S233), K246 (= K234), R247 (= R235)
- binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (≠ D43), Y187 (= Y175), N191 (= N179), R205 (= R193), W209 (≠ H197)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
39% identity, 96% coverage: 6:453/468 of query aligns to 4:464/468 of P27000
- R358 (= R347) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
32% identity, 97% coverage: 5:460/468 of query aligns to 4:502/502 of 6brlA
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
33% identity, 57% coverage: 7:273/468 of query aligns to 7:268/290 of 4a91A
- active site: S11 (= S11), K229 (= K234)
- binding glutamic acid: R7 (= R7), A9 (= A9), S11 (= S11), E43 (≠ D43), Y170 (= Y175), R188 (= R193), L192 (≠ H197)
- binding zinc ion: C99 (= C99), C101 (= C101), Y113 (= Y118), C117 (= C122)
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
35% identity, 49% coverage: 7:236/468 of query aligns to 19:243/308 of P27305
- E55 (≠ D43) binding L-glutamate
- Y182 (= Y175) binding L-glutamate
- R200 (= R193) binding L-glutamate
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
35% identity, 47% coverage: 6:224/468 of query aligns to 13:232/455 of 3aiiA
Sites not aligning to the query:
4h3sA The structure of glutaminyl-tRNA synthetase from saccharomyces cerevisiae (see paper)
34% identity, 26% coverage: 5:125/468 of query aligns to 39:158/585 of 4h3sA
Sites not aligning to the query:
Query Sequence
>WP_015798930.1 NCBI__GCF_000023265.1:WP_015798930.1
MAPERVRFAPSPTGMFHVGSARTALYNWLIARQSGGTFILRIDDTDEERNREAWVDGILR
AMEWLGLDWDEGPLRQSSRAERYRAVAERLVSEGHAYWCDCTRERVEARKQPGEAPGYDG
FCRHRGLGPGPGRALRFRVPDHDVVVTDLVRGEVRFPAGSIEDFVIVKSTGQPLYVLANV
VDDIDLAITLVLRAEEHLPTTPKAVLLHEALAAPPPRFAHLSFLVNDQRRKLSKRRDRVA
VEDYRQMGILPWALLNYVALLGWSPGTDEEFFSREELIARFDLARVQHSPAFFDERKLLA
FNQHYLQALPLAELLEQGHDHFASIGAFATQQATELLTQLLPLVVPRARTLSEAAAMSAF
LFADPPIAPNEIEALRSESASWLGAIAARLGELERFDAPSIEAALRALADELGTSLRHLQ
APLRVAVTGSRVGPPLFESLALVGPEATLRRIHALRVTLEDRTQAHAG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory