SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_015798939.1 NCBI__GCF_000023265.1:WP_015798939.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
50% identity, 99% coverage: 1:306/308 of query aligns to 2:306/306 of 4whxA

query
sites
4whxA

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active site: F37 (= F37), K160 (= K159), E194 (= E193), L217 (= L217)
binding alanine: Y97 (= Y96), K160 (= K159), A258 (= A258)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
52% identity, 97% coverage: 8:307/308 of query aligns to 6:305/305 of 2ej0B

query
sites
2ej0B

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active site: F35 (= F37), G37 (= G39), K158 (= K159), E192 (= E193), L215 (= L217)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R60), Y163 (= Y164), E192 (= E193), G195 (= G196), E196 (= E197), L215 (= L217), G217 (= G219), I218 (= I220), T219 (= T221), G254 (= G256), T255 (= T257)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
50% identity, 97% coverage: 9:307/308 of query aligns to 5:304/304 of 1iyeA

query
sites
1iyeA

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active site: F33 (= F37), G35 (= G39), K156 (= K159), A157 (≠ V160), E190 (= E193), L214 (= L217)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R60), Y92 (= Y96), Y126 (= Y129), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), N195 (= N198), L214 (= L217), G216 (= G219), I217 (= I220), T218 (= T221), G253 (= G256), T254 (= T257), A255 (= A258)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
50% identity, 97% coverage: 9:307/308 of query aligns to 5:304/304 of 1iydA

query
sites
1iydA

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active site: F33 (= F37), G35 (= G39), K156 (= K159), A157 (≠ V160), E190 (= E193), L214 (= L217)
binding glutaric acid: Y92 (= Y96), Y126 (= Y129), A255 (= A258)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), L214 (= L217), G216 (= G219), I217 (= I220), T218 (= T221), T254 (= T257)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
50% identity, 97% coverage: 9:307/308 of query aligns to 5:304/304 of 1i1mA

query
sites
1i1mA

I

I

W

W

M

F

D

N

G

G

E

E

V

M

V

V

P

R

W

W

G

E

E

D

A

A

K

K

V

V

H

H

V

V

L

M

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

Y

T

G

S

V

V

F

F

E

E

G

G

I

I

R

R

A

C

Y

Y

D

D

T

S

P

H

R

K

G

G

S

P

A

V

V

V

F

F

R

R

L

H

R

R

E

E

H

H

L

M

V

Q

R
|
R

L

L

H

H

R

D

S

S

A

A

R

K

M

I

L

Y

L

R

M

F

E

P

I

V

P

S

F

Q

S

S

V

I

D

D

E

E

L

L

I

M

E

E

A

A

T

C

R

R

T

D

V

V

V

I

A

R

A

K

N

N

G

N

E

L

P

T

A

S

C

A

Y
|
Y

I

I

R

R

P

P

V

L

A

I

F

F

T

V

A

G

Y

D

G

V

E

G

M

M

G

G

L

V

S

N

P

P

L

P

S

A

S

G

-

Y

S

S

I

T

S

D

V

V

S

I

I

I

A

A

T

A

W

F

P

P

W

W

G

G

A

A

Y

Y

L

L

G

G

D

A

E

E

G

A

I

L

A

E

K

Q

G

G

V

I

R

D

A

A

K

M

V

V

S

S

W

W

R

N

R

R

H

A

D

A

P

P

N

N

V

T

I

I

P

P

P

T

A

A

K
|
K

V

A

T

G

G

G

G

N

Y
|
Y

V

L

N

S

S

S

A

L

L

L

A

V

K

G

A

S

E

E

A

A

I

R

K

R

A

H

G

G

Y

Y

D

Q

E

E

A

G

I

I

L

A

L

L

S

D

P

V

Q

N

G

G

Y

Y

V

I

S

S

E
|
E

C

G

T

A

G
|
G

E
|
E

N

N

L

L

F

F

A

E

V

V

F

K

D

D

G

G

A

V

I

L

V

F

T

T

P

P

I

F

A

T

A

S

G

S

A

A

L
|
L

A

P

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

A

I

I

M

I

T

K

L

L

A

A

A

K

D

E

A

L

G

G

I

I

P

E

V

V

R

R

E

E

A

Q

N

V

L

L

L

S

R

R

S

E

D

S

L

L

Y

Y

I

L

A

A

D

D

E

E

V

V

F

F

L

M

S

S

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

V

T

P

P

I

V

A

R

S

S

V

V

D

D

D

G

R

I

P

Q

V

V

G

G

T

E

G

G

E

R

P

C

G

G

P

P

L

V

A

T

R

K

E

R

L

I

Q

Q

R

Q

R

A

Y

F

A

G

A

L

V

F

H

T

G

G

E

E

D

T

P

E

Q

D

H

K

E

W

S

G

W

W

L

L

T

D

Y

Q

V

V

H

N

active site: K156 (= K159)
binding 4-methyl valeric acid: Y92 (= Y96), K156 (= K159), T254 (= T257), A255 (= A258)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), L214 (= L217), G216 (= G219), I217 (= I220), T218 (= T221), G253 (= G256), T254 (= T257)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
50% identity, 97% coverage: 9:307/308 of query aligns to 5:304/304 of 1i1lA

query
sites
1i1lA

I

I

W

W

M

F

D

N

G

G

E

E

V

M

V

V

P

R

W

W

G

E

E

D

A

A

K

K

V

V

H

H

V

V

L

M

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

Y

T

G

S

V

V

F

F

E

E

G

G

I

I

R

R

A

C

Y

Y

D

D

T

S

P

H

R

K

G

G

S

P

A

V

V

V

F

F

R

R

L

H

R

R

E

E

H

H

L

M

V

Q

R
|
R

L

L

H

H

R

D

S

S

A

A

R

K

M

I

L

Y

L

R

M

F

E

P

I

V

P

S

F

Q

S

S

V

I

D

D

E

E

L

L

I

M

E

E

A

A

T

C

R

R

T

D

V

V

V

I

A

R

A

K

N

N

G

N

E

L

P

T

A

S

C

A

Y
|
Y

I

I

R

R

P

P

V

L

A

I

F

F

T

V

A

G

Y

D

G

V

E

G

M

M

G

G

L

V

S

N

P

P

L

P

S

A

S

G

-

Y

S

S

I

T

S

D

V

V

S

I

I

I

A

A

T

A

W

F

P

P

W

W

G

G

A

A

Y

Y

L

L

G

G

D

A

E

E

G

A

I

L

A

E

K

Q

G

G

V

I

R

D

A

A

K

M

V

V

S

S

W

W

R

N

R

R

H

A

D

A

P

P

N

N

V

T

I

I

P

P

P

T

A

A

K
|
K

V

A

T

G

G

G

G

N

Y
|
Y

V

L

N

S

S

S

A

L

L

L

A

V

K

G

A

S

E

E

A

A

I

R

K

R

A

H

G

G

Y

Y

D

Q

E

E

A

G

I

I

L

A

L

L

S

D

P

V

Q

N

G

G

Y

Y

V

I

S

S

E
|
E

C

G

T

A

G
|
G

E

E

N

N

L

L

F

F

A

E

V

V

F

K

D

D

G

G

A

V

I

L

V

F

T

T

P

P

I

F

A

T

A

S

G

S

A

A

L

L

A

P

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

A

I

I

M

I

T

K

L

L

A

A

A

K

D

E

A

L

G

G

I

I

P

E

V

V

R

R

E

E

A

Q

N

V

L

L

L

S

R

R

S

E

D

S

L

L

Y

Y

I

L

A

A

D

D

E

E

V

V

F

F

L

M

S

S

G

G

T
|
T

A
|
A

A

A

E

E

V

I

V

T

P

P

I

V

A

R

S

S

V

V

D

D

D

G

R

I

P

Q

V

V

G

G

T

E

G

G

E

R

P

C

G

G

P

P

L

V

A

T

R

K

E

R

L

I

Q

Q

R

Q

R

A

Y

F

A

G

A

L

V

F

H

T

G

G

E

E

D

T

P

E

Q

D

H

K

E

W

S

G

W

W

L

L

T

D

Y

Q

V

V

H

N

active site: K156 (= K159)
binding 2-methylleucine: Y92 (= Y96), K156 (= K159), T254 (= T257), A255 (= A258)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), G216 (= G219), I217 (= I220), T218 (= T221), T254 (= T257)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
50% identity, 97% coverage: 8:307/308 of query aligns to 6:297/297 of 2ej3A

query
sites
2ej3A

V

L

I

I

W

W

M

M

D

N

G

G

E

A

V

F

V

V

P

P

W

Q

G

E

E

E

A

A

K

K

V

T

H

S

V

V

L

L

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

Y

T

G

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

D

E

T

T

P

A

R

K

G

G

S

P

A

A

V

I

F

F

R

R

L

L

R

K

E

E

H

H

L

V

V

K

R
|
R

L

F

H

Y

R

N

S

S

A

A

R

K

M

V

L

L

L

R

M

M

E

E

I

I

P

P

F

F

S

A

V

P

D

E

E

E

L

L

I

E

E

E

A

A

T

I

R

K

T

E

V

V

A

R

N

N

G

G

E

Y

P

R

A

S

C

C

Y

Y

I

I

R

R

P

P

V

L

A

A

F

W

T

M

A

G

Y

A

G

K

E

A

M

L

G

G

L

V

S

N

P

P

L

L

-

P

S

N

S

P

I

A

S

E

V

V

S

M

I

V

A

A

T

A

W

W

P

E

W

W

G

-

A

-

Y

-

L

-

G

-

D

-

E

-

G

-

I

V

A

R

K

K

G

G

V

A

R

R

A

L

K

I

V

T

S

S

W

W

R

A

R

R

H

F

D

P

P

A

N

N

V

V

I

M

P

P

P

G

A

K

A

A

K
|
K

V

V

T

G

G

G

G

N

Y
|
Y

V

V

N

N

S

S

A

A

L

L

A

A

K

K

A

M

E

E

A

A

I

V

K

A

A

A

G

G

Y

A

D

D

E

E

A

A

I

L

L

L

S

D

P

E

Q

E

G

G

Y

Y

V

V

S

A

E
|
E

C

G

T

S

G
|
G

E

E

N

N

L

L

F

F

A

F

V

V

F

R

D

D

G

G

A

V

I

I

V

Y

T

A

P

L

P

E

I

H

A

S

A

V

G

N

A

-

L
|
L

A

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

I

V

M

I

T

R

L

I

A

A

A

K

D

D

A

L

G

G

I

Y

P

E

V

V

R

Q

E

V

A

V

N

R

L

A

L

T

R

R

S

D

D

Q

L

L

Y

Y

I

M

A

A

D

D

E

E

V

V

F

F

L

M

S

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

V

V

T

P

P

I

V

A

S

S

M

V

I

D

D

D

W

R

R

P

P

V

I

G

G

T

K

G

G

E

T

P

A

G

G

P

P

L

V

A

A

R

L

E

R

L

L

Q

R

R

E

R

V

Y

Y

Y

L

A

E

A

A

V

V

H

T

G

G

E

R

D

R

P

P

Q

E

H

Y

E

E

S

G

W

W

L

L

T

T

Y

Y

V

V

H

N

active site: F35 (= F37), G37 (= G39), K150 (= K159), E184 (= E193), L207 (= L217)
binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G196), G246 (= G256), T247 (= T257), A248 (= A258)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), L207 (= L217), G209 (= G219), I210 (= I220), T211 (= T221), G246 (= G256), T247 (= T257)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
50% identity, 97% coverage: 8:307/308 of query aligns to 6:297/297 of 2eiyA

query
sites
2eiyA

V

L

I

I

W

W

M

M

D

N

G

G

E

A

V

F

V

V

P

P

W

Q

G

E

E

E

A

A

K

K

V

T

H

S

V

V

L

L

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

Y

T

G

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

D

E

T

T

P

A

R

K

G

G

S

P

A

A

V

I

F

F

R

R

L

L

R

K

E

E

H

H

L

V

V

K

R
|
R

L

F

H

Y

R

N

S

S

A

A

R

K

M

V

L

L

L

R

M

M

E

E

I

I

P

P

F

F

S

A

V

P

D

E

E

E

L

L

I

E

E

E

A

A

T

I

R

K

T

E

V

V

A

R

N

N

G

G

E

Y

P

R

A

S

C

C

Y
|
Y

I

I

R

R

P

P

V

L

A

A

F

W

T

M

A

G

Y

A

G

K

E

A

M

L

G

G

L

V

S

N

P

P

L

L

-

P

S

N

S

P

I

A

S

E

V

V

S

M

I

V

A

A

T

A

W

W

P

E

W

W

G

-

A

-

Y

-

L

-

G

-

D

-

E

-

G

-

I

V

A

R

K

K

G

G

V

A

R

R

A

L

K

I

V

T

S

S

W

W

R

A

R

R

H

F

D

P

P

A

N

N

V

V

I

M

P

P

P

G

A

K

A

A

K
|
K

V

V

T

G

G

G

G

N

Y
|
Y

V

V

N

N

S

S

A

A

L

L

A

A

K

K

A

M

E

E

A

A

I

V

K

A

A

A

G

G

Y

A

D

D

E

E

A

A

I

L

L

L

S

D

P

E

Q

E

G

G

Y

Y

V

V

S

A

E
|
E

C

G

T

S

G
|
G

E
|
E

N

N

L

L

F

F

A

F

V

V

F

R

D

D

G

G

A

V

I

I

V

Y

T

A

P

L

P

E

I

H

A

S

A

V

G

N

A

-

L
|
L

A

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

I

V

M

I

T

R

L

I

A

A

A

K

D

D

A

L

G

G

I

Y

P

E

V

V

R

Q

E

V

A

V

N

R

L

A

L

T

R

R

S

D

D

Q

L

L

Y

Y

I

M

A

A

D

D

E

E

V

V

F

F

L

M

S

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

V

V

T

P

P

I

V

A

S

S

M

V

I

D

D

D

W

R

R

P

P

V

I

G

G

T

K

G

G

E

T

P

A

G

G

P

P

L

V

A

A

R

L

E

R

L

L

Q

R

R

E

R

V

Y

Y

Y

L

A

E

A

A

V

V

H

T

G

G

E

R

D

R

P

P

Q

E

H

Y

E

E

S

G

W

W

L

L

T

T

Y

Y

V

V

H

N

active site: F35 (= F37), G37 (= G39), K150 (= K159), E184 (= E193), L207 (= L217)
binding 4-methyl valeric acid: F35 (= F37), Y94 (= Y96), T247 (= T257), A248 (= A258)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), E188 (= E197), L207 (= L217), G209 (= G219), I210 (= I220), T211 (= T221), G246 (= G256), T247 (= T257)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
50% identity, 97% coverage: 8:307/308 of query aligns to 6:297/297 of 1wrvA

query
sites
1wrvA

V

L

I

I

W

W

M

M

D

N

G

G

E

A

V

F

V

V

P

P

W

Q

G

E

E

E

A

A

K

K

V

T

H

S

V

V

L

L

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

Y

T

G

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

D

E

T

T

P

A

R

K

G

G

S

P

A

A

V

I

F

F

R

R

L

L

R

K

E

E

H

H

L

V

V

K

R
|
R

L

F

H

Y

R

N

S

S

A

A

R

K

M

V

L

L

L

R

M

M

E

E

I

I

P

P

F

F

S

A

V

P

D

E

E

E

L

L

I

E

E

E

A

A

T

I

R

K

T

E

V

V

A

R

N

N

G

G

E

Y

P

R

A

S

C

C

Y

Y

I

I

R

R

P

P

V

L

A

A

F

W

T

M

A

G

Y

A

G

K

E

A

M

L

G

G

L

V

S

N

P

P

L

L

-

P

S

N

S

P

I

A

S

E

V

V

S

M

I

V

A

A

T

A

W

W

P

E

W

W

G

-

A

-

Y

-

L

-

G

-

D

-

E

-

G

-

I

V

A

R

K

K

G

G

V

A

R

R

A

L

K

I

V

T

S

S

W

W

R

A

R

R

H

F

D

P

P

A

N

N

V

V

I

M

P

P

P

G

A

K

A

A

K
|
K

V

V

T

G

G

G

G

N

Y
|
Y

V

V

N

N

S

S

A

A

L

L

A

A

K

K

A

M

E

E

A

A

I

V

K

A

A

A

G

G

Y

A

D

D

E

E

A

A

I

L

L

L

S

D

P

E

Q

E

G

G

Y

Y

V

V

S

A

E
|
E

C

G

T

S

G
|
G

E

E

N

N

L

L

F

F

A

F

V

V

F

R

D

D

G

G

A

V

I

I

V

Y

T

A

P

L

P

E

I

H

A

S

A

V

G

N

A

-

L
|
L

A

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

I

V

M

I

T

R

L

I

A

A

A

K

D

D

A

L

G

G

I

Y

P

E

V

V

R

Q

E

V

A

V

N

R

L

A

L

T

R

R

S

D

D

Q

L

L

Y

Y

I

M

A

A

D

D

E

E

V

V

F

F

L

M

S

T

G

G

T
|
T

A

A

E

E

V

V

V

T

P

P

I

V

A

S

S

M

V

I

D

D

D

W

R

R

P

P

V

I

G

G

T

K

G

G

E

T

P

A

G

G

P

P

L

V

A

A

R

L

E

R

L

L

Q

R

R

E

R

V

Y

Y

Y

L

A

E

A

A

V

V

H

T

G

G

E

R

D

R

P

P

Q

E

H

Y

E

E

S

G

W

W

L

L

T

T

Y

Y

V

V

H

N

active site: F35 (= F37), G37 (= G39), K150 (= K159), E184 (= E193), L207 (= L217)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), L207 (= L217), G209 (= G219), I210 (= I220), T211 (= T221), T247 (= T257)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
50% identity, 97% coverage: 8:307/308 of query aligns to 6:294/294 of 2ej2A

query
sites
2ej2A

V

L

I

I

W

W

M

M

D

N

G

G

E

A

V

F

V

V

P

P

W

Q

G

E

E

E

A

A

K

K

V

T

H

S

V

V

L

L

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

Y

T

G

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

D

E

T

T

P

A

R

K

G

G

S

P

A

A

V

I

F

F

R

R

L

L

R

K

E

E

H

H

L

V

V

K

R
|
R

L

F

H

Y

R

N

S

S

A

A

R

K

M

V

L

L

L

R

M

M

E

E

I

I

P

P

F

F

S

A

V

P

D

E

E

E

L

L

I

E

E

E

A

A

T

I

R

K

T

E

V

V

A

R

N

N

G

G

E

Y

P

R

A

S

C

C

Y
|
Y

I

I

R

R

P

P

V

L

A

A

F

W

T

M

A

G

Y

A

G

K

E

A

M

L

G

G

L

V

S

N

P

P

L

L

-

P

S

N

S

P

I

A

S

E

V

V

S

M

I

V

A

A

T

A

W

W

P

E

W

W

G

G

A

A

Y

-

L

-

G

-

D

-

E

-

G

-

I

-

A

-

K

-

G

-

V

-

R

R

A

L

K

I

V

T

S

S

W

W

R

A

R

R

H

F

D

P

P

A

N

N

V

V

I

M

P

P

P

G

A

K

A

A

K
|
K

V

V

T

G

G

G

G

N

Y
|
Y

V

V

N

N

S

S

A

A

L

L

A

A

K

K

A

M

E

E

A

A

I

V

K

A

A

A

G

G

Y

A

D

D

E

E

A

A

I

L

L

L

S

D

P

E

Q

E

G

G

Y

Y

V

V

S

A

E
|
E

C

G

T

S

G
|
G

E
|
E

N

N

L

L

F

F

A

F

V

V

F

R

D

D

G

G

A

V

I

I

V

Y

T

A

P

L

P

E

I

H

A

S

A

V

G

N

A

-

L
|
L

A

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

I

V

M

I

T

R

L

I

A

A

A

K

D

D

A

L

G

G

I

Y

P

E

V

V

R

Q

E

V

A

V

N

R

L

A

L

T

R

R

S

D

D

Q

L

L

Y

Y

I

M

A

A

D

D

E

E

V

V

F

F

L

M

S

T

G

G

T
|
T

A
|
A

A

A

E

E

V

V

V

T

P

P

I

V

A

S

S

M

V

I

D

D

D

W

R

R

P

P

V

I

G

G

T

K

G

G

E

T

P

A

G

G

P

P

L

V

A

A

R

L

E

R

L

L

Q

R

R

E

R

V

Y

Y

Y

L

A

E

A

A

V

V

H

T

G

G

E

R

D

R

P

P

Q

E

H

Y

E

E

S

G

W

W

L

L

T

T

Y

Y

V

V

H

N

active site: F35 (= F37), G37 (= G39), K147 (= K159), E181 (= E193), L204 (= L217)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R60), Y94 (= Y96), Y152 (= Y164), E181 (= E193), G184 (= G196), E185 (= E197), L204 (= L217), G206 (= G219), I207 (= I220), T208 (= T221), T244 (= T257), A245 (= A258)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
45% identity, 97% coverage: 9:307/308 of query aligns to 9:301/301 of 6thqB

query
sites
6thqB

I

V

W

W

M

L

D

D

G

G

E

R

V

L

V

V

P

D

W

E

G

E

E

E

A

A

K

K

V

V

H

T

V

V

L

L

T

S

H

P

A

S

L

L

H

N

Y

Y

G

G

Y

F

G

G

V

V

F
|
F

E

E

G

G

I

I

R

R

A

A

Y

Y

D

W

T

N

P

G

R

E

G

N

S

L

A

Y

V

V

F

F

R

R

L

L

R

R

E

D

H

H

L

M

V

E

R
|
R

L

L

H

L

R

R

S

S

A

A

R

K

M

I

L

I

L

G

M

L

E

D

I

V

P

P

F

Y

S

T

V

A

D

E

E

E

L

L

I

S

E

K

A

A

T

V

R

V

T

E

V

T

V

V

A

R

A

A

N

N

G

G

-

F

E

K

P

E

A

D

C

L

Y
|
Y

I

I

R

R

P

P

V

V

A

A

F

Y

T

I

A

S

Y

K

G

P

E

Q

M

I

G

S

L

L

S

D

P

V

L

R

S

G

S

L

S

Q

I

A

S

S

V

V

S

A

I

I

A

A

T

A

W

I

P

P

W

F

G

G

A

K

Y

Y

L

L

G

K

D

V

E

E

G

G

I

-

A

-

K

-

G

-

V

V

R

R

A

A

K

A

V

V

S

V

S

S

W

W

R

R

H

V

D

H

P

T

N

S

V

M

I

M

P

P

P

V

A

M

A

A

K
|
K

V

A

T

T

G

G

G

I

Y
|
Y

V

L

N

N

S

S

A

I

L

M

A

A

K

A

A

V

E

E

A

A

I

R

K

A

A

R

G

G

Y

Y

D

D

E

E

A

A

I

I

L

M

L

L

S

N

P

A

Q

E

G

G

Y

K

V

V

S

V

E
|
E

C

G

T

S

G
|
G

E
|
E

N
|
N

L

I

F

F

A

I

V

V

F

R

D

R

G

G

A

V

I

L

V

M

T

T

P

P

I

L

A

E

A

D

G

G

A

I

L
|
L

A

E

G
|
G

I
|
I

T
|
T

R

R

Q

E

T

T

I

V

M

I

T

S

L

I

A

A

A

G

D

D

A

L

G

G

I

I

P

P

V

L

R

L

E

E

A

K

N

S

L

I

L

T

R

R

S

E

D

E

L

L

Y

Y

I

A

A

A

D

D

E

E

V

A

F

F

L

F

S

V

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

V

T

P

P

I

I

A

I

S

E

V

I

D

D

D

G

R

R

P

V

V

L

G

-

T

-

G

-

E

Q

P

R

G

G

P

P

L

I

A

T

R

Q

E

K

L

I

Q

A

R

E

R

T

Y

Y

Y

R

A

R

A

I

V

V

H

L

G

G

E

K

D

E

P

E

Q

K

H

Y

E

L

S

P

W

W

L

L

T

T

Y

P

V

V

H

Y

active site: F37 (= F37), K156 (= K159), E190 (= E193), L214 (= L217)
binding pyridoxal-5'-phosphate: R60 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), N195 (= N198), L214 (= L217), G216 (= G219), I217 (= I220), T218 (= T221), T254 (= T257)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R60), Y97 (= Y96), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), N195 (= N198), G216 (= G219), I217 (= I220), T218 (= T221), G253 (= G256), T254 (= T257), A255 (= A258)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
43% identity, 93% coverage: 9:295/308 of query aligns to 4:284/290 of 5mr0D

query
sites
5mr0D

I

V

W

Y

M

M

D

D

G

G

E

E

V

F

V

V

P

P

W

E

G

N

E

E

A

A

K

K

V

V

H

S

V

I

L

F

T

D

H

H

A

G

L

F

H

L

Y

Y

G

G

Y

D

G

G

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

D

N

T

-

P

-

R

-

G

-

S

G

A

R

V

V

F

F

R

R

L

L

R

K

E

E

H

H

L

I

V

D

R
|
R

L

L

H

Y

R

D

S

S

A

A

R

K

M

A

L

I

L

D

M

L

E

E

I

I

P

P

F

I

S

T

V

K

D

E

E

E

L

F

I

M

E

E

A

I

T

I

R

L

T

E

V

T

V

L

A

R

A

K

N

N

G

N

E

L

P

R

A

D

C

A

Y

Y

I

I

R

R

P

P

V

I

A

V

F

T

T

R

A

G

Y

I

G

G

E

D

M

L

G
|
G

L
|
L

S

D

P

P

L

R

S

K

-

C

S

Q

S

N

I

P

S

S

V

I

S

I

I

V

A

I

T

T

W

K

P

P

W

W

G

G

A

K

Y

L

L

Y

G

G

D

D

E

L

G

-

I

Y

A

E

K

K

G

G

V

L

R

T

A

A

K

I

V

T

S

V

S

A

W

V

R

R

H

N

D

S

P

F

N

D

V

A

I

L

P

P

A

N

A

I

K
|
K

V

-

T

S

G

L

G

N

Y
|
Y

V

L

N

N

S

N

A

I

L

L

A

A

K

K

A

I

E

E

A

A

I

N

K

A

A

K

G

G

Y

G

D

D

E

E

A

A

I

I

L

F

L

L

S

D

P

R

Q

N

G

G

Y

Y

V

V

S

S

E
|
E

C

G

T

S

G
|
G

E
x
D

N

N

L

I

F

F

A

V

V

V

F

K

D

N

G

G

A

A

I

I

V

T

T

T

P

P

I

-

A

T

A

I

G

N

A

N

L
|
L

A

R

G

G

I
|
I

T
|
T

R

R

Q

E

T

A

I

V

M

I

T

E

L

I

A

I

A

N

D

R

A

L

G

G

I

I

P

P

V

F

R

K

E

E

A

T

N

N

L

I

L

G

R

L

S

Y

D

D

L

L

Y

Y

I

T

A

A

D

D

E

E

V

V

F

F

L

V

S

T

G
|
G

T
|
T

A

A

E

E

V

I

V

A

P

P

I

I

A

V

S

V

V

I

D

D

D

G

R

R

P

K

V

I

G

G

T

D

G

G

E

K

P

P

G

G

P

E

L

I

A

T

R

R

E

K

L

L

Q

M

R

E

Y

F

Y

S

A

K

A

L

V

T

H

E

G

S

E

E

active site: F32 (= F37), G34 (= G39), K150 (= K159), E183 (= E193), L206 (= L217)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R60), G100 (= G109), L101 (= L110), K150 (= K159), Y154 (= Y164), E183 (= E193), G186 (= G196), D187 (≠ E197), L206 (= L217), I209 (= I220), T210 (= T221), G245 (= G256), T246 (= T257)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
43% identity, 94% coverage: 8:295/308 of query aligns to 4:285/290 of 5e25A

query
sites
5e25A

V

L

I

V

W

Y

M

M

D

N

G

G

E

E

V

F

V

V

P

P

W

E

G

S

E

Q

A

A

K

K

V

V

H

S

V

V

L

F

T

D

H

H

A

G

L

F

H

L

Y

Y

G

G

Y

D

G

G

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

D

N

T

-

P

-

R

-

G

-

S

G

A

K

V

V

F

F

R

K

L

L

R

Y

E

E

H

H

L

I

V

D

R
|
R

L

L

H

Y

R

D

S

C

A

A

R

R

M

V

L

I

L

D

M

L

E

K

I

I

P

P

F

L

S

S

V

K

D

E

E

E

L

F

I

A

E

E

A

A

T

I

R

L

T

E

V

T

V

L

A

R

N

N

G

N

E

L

P

R

A

D

C

A

Y
|
Y

I

I

R

R

P

P

V

I

A

V

F

T

T

R

A

G

Y

A

G

G

E

D

M

L

G

G

L

L

S

D

P

P

L

R

S

K

S

C

-

P

S

S

I

P

S

N

V

V

S

I

I

I

A

I

T

T

W

K

P

P

W

W

G

G

A

K

Y

L

L

Y

G

G

D

D

E

L

G

-

I

Y

A

E

K

K

G

G

V

L

R

K

A

A

K

I

V

T

S

V

S

A

W

I

R

R

H

N

D

A

P

I

N

D

V

S

I

L

P

P

A

N

A

I

K
|
K

V

-

T

S

G

L

G

N

Y
|
Y

V

L

N

N

S

N

A

I

L

L

A

A

K

K

A

I

E

E

A

A

I

N

K

A

A

K

G

G

Y

G

D

D

E

E

A

A

I

I

L

F

L

L

S

D

P

H

Q

N

G

G

Y

Y

V

I

S

S

E
|
E

C

G

T

S

G
|
G

E
x
D

N

N

L

I

F

F

A

I

V

V

F

K

D

N

G

G

A

T

I

I

V

T

T

T

P

P

I

-

A

T

A

L

G

N

A

N

L
|
L

A

K

G
|
G

I
|
I

T
|
T

R

R

Q

Q

T

V

I

V

M

I

T

E

L

L

A

I

A

N

D

E

A

L

G

E

I

I

P

P

V

F

R

R

E

E

A

A

N

N

L

I

L

G

R

L

S

F

D

D

L

L

Y

Y

I

S

A

A

D

D

E

E

V

I

F

F

L

V

S

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

V

A

P

P

I

V

A

T

S

Y

V

I

D

D

D

G

R

R

P

T

V

V

G

G

T

N

G

G

E

K

P

P

G

G

P

K

L

V

A

T

R

K

E

M

L

L

Q

M

R

E

R

K

Y

F

Y

R

A

E

A

R

V

T

H

E

G

N

E

E

active site: F33 (= F37), G35 (= G39), K151 (= K159), E184 (= E193), L207 (= L217)
binding 2-oxoglutaric acid: Y88 (= Y96), K151 (= K159), T247 (= T257), A248 (= A258)
binding pyridoxal-5'-phosphate: R52 (= R60), K151 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), D188 (≠ E197), L207 (= L217), G209 (= G219), I210 (= I220), T211 (= T221), G246 (= G256), T247 (= T257)

6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
36% identity, 97% coverage: 10:307/308 of query aligns to 8:307/308 of 6h65C

query
sites
6h65C

W

W

M

M

D

N

G

G

E

Q

V

C

V

I

P

P

W

W

G

D

E

Q

A

C

K

S

V

L

H

H

V

V

L

S

T

T

H

Q

A

A

L

A

H

F

Y

F

G

G

Y

A

G

S

V

L

F
|
F

E

E

G

G

I

V

R

R

A

A

Y

Y

-

W

D

N

T

A

P

E

R

R

G

E

S

Q

A

L

-

Y

V

V

F

F

R

R

L

L

R

D

E

E

H

H

L

L

V

R

R
|
R

L

L

H

E

R

Q

S

S

A

A

R

K

M

M

L

L

L

R

M

M

E

K

I

L

P

S

F

M

S

P

V

I

D

A

E

D

L

I

I

R

E

Q

A

G

T

V

R

L

T

E

V

L

A

R

A

A

N

N

-

E

-

F

-

R

G

S

E

D

P

V

A

H

C

L

Y

Y

I

V

R

A

P

S

V

Y

A

F

F

G

T

I

A

N

Y

H

G

D

E

P

M

D

G

P

L

L

S

F

P

P

L

T

S

D

S

T

I

-

S

-

V

-

S

-

I

-

A

-

T

-

W

-

P

-

W

-

G

G

A

V

Y

Y

L

V

G

T

D

G

E

T

G

A

I

V

A

S

K

R

-

L

-

P

-

L

-

V

-

H

-

T

G

G

V

I

R

S

A

A

K

C

V

M

S

S

W

W

R

R

H

I

D

S

P

D

N

D

V

S

I

V

P

P

A

R

A

I

K
|
K

V

I

T

G

G

A

G

N

Y
|
Y

V

Q

N

N

S

S

A

R

L

L

A

A

K

Q

A

T

E

E

A

A

I

R

K

V

A

N

G

G

Y

Y

D

H

E

T

A

S

I

V

L

L

S

N

P

S

Q

R

G

G

Y

K

V

V

S

S

E
|
E

C

T

T

P

G

G

E
x
A

N

C

L

L

F

L

A

M

V

V

F

R

D

D

G

G

A

R

I

V

V

I

T

S

P

P

I

V

A

T

A

A

G

D

A

I

L
|
L

A

E

G
x
S

I
x
V

T
|
T

R

R

Q

K

T

T

I

L

M

M

T

S

L

L

A

S

-

E

A

A

D

E

A

L

G

D

I

S

P

P

V

V

R

I

E

E

A

R

N

D

L

M

L

D

R

R

S

T

D

E

L

L

Y

Y

I

I

A

A

D

E

E

E

V

V

F

F

L

L

S

C

G
|
G

T
|
T

A

I

A

A

E

E

V

I

V

L

P

P

I

V

A

T

S

T

V

I

D

D

D

R

R

I

P

Q

V

V

G

G

T

D

G

G

E

E

P

V

G

G

P

P

L

V

A

T

R

R

E

R

L

L

Q

Q

R

E

R

L

Y

Y

Y

F

A

G

A

V

V

T

H

S

G

G

E

Q

D

L

P

E

Q

A

H

Y

E

K

S

S

W

W

L

L

T

L

Y

P

V

V

H

Y

active site: F35 (= F37), K158 (= K159), E192 (= E193), L216 (= L217)
binding pyridoxal-5'-phosphate: R60 (= R60), K158 (= K159), Y163 (= Y164), E192 (= E193), A196 (≠ E197), L216 (= L217), S218 (≠ G219), V219 (≠ I220), T220 (= T221), G256 (= G256), T257 (= T257)

6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
38% identity, 97% coverage: 7:304/308 of query aligns to 4:302/307 of 6q8eA

query
sites
6q8eA

K

K

V

Y

I

L

W

W

M

W

D

N

G

H

E

R

V

I

P

P

W

W

G

E

E

E

A

A

K

T

V

V

H

H

V

L

L

T

T

D

H

Y

A

W

L

W

H

A

Y

S

G

V

Y

T

G

A

V

V

F
|
F

E

E

G

G

I

I

R

R

A

G

Y

Y

-

W

D

N

T

N

P

A

R

E

G

G

S

E

A

M

-

Y

V

I

F

F

R

R

L

L

R

E

E

D

H

H

L

A

V

R

R
|
R

L

L

H

E

R

Q

S

S

A

M

R

Q

M

L

L

I

L

R

M

M

E

P

I

K

P

E

F

F

S

T

V

V

D

D

E

E

L

I

I

C