SitesBLAST
Comparing WP_015798939.1 NCBI__GCF_000023265.1:WP_015798939.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
50% identity, 99% coverage: 1:306/308 of query aligns to 2:306/306 of 4whxA
2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
52% identity, 97% coverage: 8:307/308 of query aligns to 6:305/305 of 2ej0B
- active site: F35 (= F37), G37 (= G39), K158 (= K159), E192 (= E193), L215 (= L217)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R60), Y163 (= Y164), E192 (= E193), G195 (= G196), E196 (= E197), L215 (= L217), G217 (= G219), I218 (= I220), T219 (= T221), G254 (= G256), T255 (= T257)
1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
50% identity, 97% coverage: 9:307/308 of query aligns to 5:304/304 of 1iyeA
- active site: F33 (= F37), G35 (= G39), K156 (= K159), A157 (≠ V160), E190 (= E193), L214 (= L217)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R60), Y92 (= Y96), Y126 (= Y129), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), N195 (= N198), L214 (= L217), G216 (= G219), I217 (= I220), T218 (= T221), G253 (= G256), T254 (= T257), A255 (= A258)
1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
50% identity, 97% coverage: 9:307/308 of query aligns to 5:304/304 of 1iydA
- active site: F33 (= F37), G35 (= G39), K156 (= K159), A157 (≠ V160), E190 (= E193), L214 (= L217)
- binding glutaric acid: Y92 (= Y96), Y126 (= Y129), A255 (= A258)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), L214 (= L217), G216 (= G219), I217 (= I220), T218 (= T221), T254 (= T257)
1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
50% identity, 97% coverage: 9:307/308 of query aligns to 5:304/304 of 1i1mA
- active site: K156 (= K159)
- binding 4-methyl valeric acid: Y92 (= Y96), K156 (= K159), T254 (= T257), A255 (= A258)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), L214 (= L217), G216 (= G219), I217 (= I220), T218 (= T221), G253 (= G256), T254 (= T257)
1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
50% identity, 97% coverage: 9:307/308 of query aligns to 5:304/304 of 1i1lA
- active site: K156 (= K159)
- binding 2-methylleucine: Y92 (= Y96), K156 (= K159), T254 (= T257), A255 (= A258)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), G216 (= G219), I217 (= I220), T218 (= T221), T254 (= T257)
2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
50% identity, 97% coverage: 8:307/308 of query aligns to 6:297/297 of 2ej3A
- active site: F35 (= F37), G37 (= G39), K150 (= K159), E184 (= E193), L207 (= L217)
- binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G196), G246 (= G256), T247 (= T257), A248 (= A258)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), L207 (= L217), G209 (= G219), I210 (= I220), T211 (= T221), G246 (= G256), T247 (= T257)
2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
50% identity, 97% coverage: 8:307/308 of query aligns to 6:297/297 of 2eiyA
- active site: F35 (= F37), G37 (= G39), K150 (= K159), E184 (= E193), L207 (= L217)
- binding 4-methyl valeric acid: F35 (= F37), Y94 (= Y96), T247 (= T257), A248 (= A258)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), E188 (= E197), L207 (= L217), G209 (= G219), I210 (= I220), T211 (= T221), G246 (= G256), T247 (= T257)
1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
50% identity, 97% coverage: 8:307/308 of query aligns to 6:297/297 of 1wrvA
- active site: F35 (= F37), G37 (= G39), K150 (= K159), E184 (= E193), L207 (= L217)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), L207 (= L217), G209 (= G219), I210 (= I220), T211 (= T221), T247 (= T257)
2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
50% identity, 97% coverage: 8:307/308 of query aligns to 6:294/294 of 2ej2A
- active site: F35 (= F37), G37 (= G39), K147 (= K159), E181 (= E193), L204 (= L217)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R60), Y94 (= Y96), Y152 (= Y164), E181 (= E193), G184 (= G196), E185 (= E197), L204 (= L217), G206 (= G219), I207 (= I220), T208 (= T221), T244 (= T257), A245 (= A258)
6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
45% identity, 97% coverage: 9:307/308 of query aligns to 9:301/301 of 6thqB
- active site: F37 (= F37), K156 (= K159), E190 (= E193), L214 (= L217)
- binding pyridoxal-5'-phosphate: R60 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), N195 (= N198), L214 (= L217), G216 (= G219), I217 (= I220), T218 (= T221), T254 (= T257)
- binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R60), Y97 (= Y96), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), N195 (= N198), G216 (= G219), I217 (= I220), T218 (= T221), G253 (= G256), T254 (= T257), A255 (= A258)
5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
43% identity, 93% coverage: 9:295/308 of query aligns to 4:284/290 of 5mr0D
- active site: F32 (= F37), G34 (= G39), K150 (= K159), E183 (= E193), L206 (= L217)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R60), G100 (= G109), L101 (= L110), K150 (= K159), Y154 (= Y164), E183 (= E193), G186 (= G196), D187 (≠ E197), L206 (= L217), I209 (= I220), T210 (= T221), G245 (= G256), T246 (= T257)
5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
43% identity, 94% coverage: 8:295/308 of query aligns to 4:285/290 of 5e25A
- active site: F33 (= F37), G35 (= G39), K151 (= K159), E184 (= E193), L207 (= L217)
- binding 2-oxoglutaric acid: Y88 (= Y96), K151 (= K159), T247 (= T257), A248 (= A258)
- binding pyridoxal-5'-phosphate: R52 (= R60), K151 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), D188 (≠ E197), L207 (= L217), G209 (= G219), I210 (= I220), T211 (= T221), G246 (= G256), T247 (= T257)
6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
36% identity, 97% coverage: 10:307/308 of query aligns to 8:307/308 of 6h65C
- active site: F35 (= F37), K158 (= K159), E192 (= E193), L216 (= L217)
- binding pyridoxal-5'-phosphate: R60 (= R60), K158 (= K159), Y163 (= Y164), E192 (= E193), A196 (≠ E197), L216 (= L217), S218 (≠ G219), V219 (≠ I220), T220 (= T221), G256 (= G256), T257 (= T257)
6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
38% identity, 97% coverage: 7:304/308 of query aligns to 4:302/307 of 6q8eA
- active site: F34 (= F37), K156 (= K159), E190 (= E193), L214 (= L217)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), S194 (≠ E197), C195 (≠ N198), L214 (= L217), S216 (≠ G219), I217 (= I220), T218 (= T221), G254 (= G256), T255 (= T257)
7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
37% identity, 97% coverage: 7:304/308 of query aligns to 4:302/309 of 7neaA
- active site: F34 (= F37), K156 (= K159), E190 (= E193), L214 (= L217)
- binding pyridoxal-5'-phosphate: R59 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), S194 (≠ E197), L214 (= L217), S216 (≠ G219), I217 (= I220), T218 (= T221), T255 (= T257)
7p3tB Transaminase of gamma-proteobacterium (see paper)
32% identity, 92% coverage: 8:290/308 of query aligns to 5:281/299 of 7p3tB
- binding pyridoxal-5'-phosphate: R53 (= R60), K153 (= K159), R157 (≠ Y164), E186 (= E193), S187 (≠ C194), A188 (≠ T195), A189 (≠ G196), S190 (≠ E197), G210 (= G219), I211 (= I220), T212 (= T221), T248 (= T257)
3ht5A Crystal structure of ilve a branched chain amino acid transaminase from mycobacterium tuberculosis (see paper)
34% identity, 94% coverage: 4:294/308 of query aligns to 12:329/335 of 3ht5A
- active site: K171 (= K159)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R68 (= R60), K171 (= K159), Y176 (= Y164), E207 (= E193), G210 (= G196), M211 (≠ E197), N212 (= N198), L235 (= L217), G237 (= G219), I238 (= I220), T239 (= T221), T281 (= T257)
3dtgA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design
33% identity, 93% coverage: 10:294/308 of query aligns to 45:357/363 of 3dtgA
- active site: K199 (= K159)
- binding O-benzylhydroxylamine: F72 (= F37), Y171 (= Y129), Y204 (= Y164)
- binding pyridoxal-5'-phosphate: R96 (= R60), R189 (≠ V153), K199 (= K159), Y204 (= Y164), E235 (= E193), G238 (= G196), L263 (= L217), I266 (= I220), T267 (= T221), T309 (= T257)
3dtfA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design (see paper)
33% identity, 93% coverage: 10:294/308 of query aligns to 45:357/363 of 3dtfA
Query Sequence
>WP_015798939.1 NCBI__GCF_000023265.1:WP_015798939.1
MAIAEGKVIWMDGEVVPWGEAKVHVLTHALHYGYGVFEGIRAYDTPRGSAVFRLREHLVR
LHRSARMLLMEIPFSVDELIEATRTVVAANGEPACYIRPVAFTAYGEMGLSPLSSSISVS
IATWPWGAYLGDEGIAKGVRAKVSSWRRHDPNVIPPAAKVTGGYVNSALAKAEAIKAGYD
EAILLSPQGYVSECTGENLFAVFDGAIVTPPIAAGALAGITRQTIMTLAADAGIPVREAN
LLRSDLYIADEVFLSGTAAEVVPIASVDDRPVGTGEPGPLARELQRRYYAAVHGEDPQHE
SWLTYVHG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory