SitesBLAST
Comparing WP_015799002.1 NCBI__GCF_000023265.1:WP_015799002.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4q9dB X-ray structure of a putative thiamin diphosphate-dependent enzyme isolated from mycobacterium smegmatis (see paper)
28% identity, 31% coverage: 334:492/506 of query aligns to 351:502/519 of 4q9dB
- active site: M374 (≠ D357), S376 (≠ A359), G401 (= G384), I403 (= I386), D432 (= D409), N459 (= N436), E461 (≠ G438), Y462 (= Y439), I464 (= I441)
- binding magnesium ion: D432 (= D409), N459 (= N436), E461 (≠ G438)
Sites not aligning to the query:
- active site: 24, 26, 27, 28, 29, 47, 48, 71, 107, 108, 110, 111, 112, 113, 161, 252, 279, 519
5d6rB Acetolactate synthase from klebsiella pneumoniae in complex with mechanism-based inhibitor
25% identity, 97% coverage: 2:492/506 of query aligns to 6:518/548 of 5d6rB
- active site: I26 (≠ N22), G28 (= G24), A29 (≠ T25), K30 (≠ S26), I31 (≠ E27), E51 (= E47), T74 (≠ H69), H113 (≠ P108), Q114 (≠ L109), S115 (≠ T110), Q163 (≠ S167), L254 (= L259), E281 (≠ D283), M386 (≠ A365), Q412 (≠ G384), M414 (≠ I386), D439 (= D409), D466 (≠ N436), G468 (= G438), Y469 (= Y439), M471 (≠ I441), V472 (≠ L442), Q475 (≠ E445)
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(Z)-2-fluoro-1-hydroxy-2-phosphonoethenyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: M386 (≠ A365), G387 (≠ L366), S388 (≠ Y367), Q412 (≠ G384), M414 (≠ I386), D439 (= D409), G440 (= G410), G468 (= G438), Y469 (= Y439), N470 (≠ R440), M471 (≠ I441)
- binding magnesium ion: R63 (= R59), Q212 (≠ E209), D439 (= D409), D466 (≠ N436), G468 (= G438)
Sites not aligning to the query:
7orxCCC jostii RHA1 thiamine diphosphate-dependent 4-hydroxybenzoylformate decarboxylase
27% identity, 31% coverage: 334:492/506 of query aligns to 354:511/531 of 7orxCCC
- active site: S379 (≠ A359), G404 (= G384), L406 (≠ I386), D431 (= D409), N458 (= N436), T460 (≠ G438), Y461 (= Y439), A463 (≠ I441), L464 (= L442), F467 (≠ E445)
- binding thiamine diphosphate: T380 (≠ R360), S381 (≠ T361), G404 (= G384), L406 (≠ I386), G430 (≠ A408), G432 (= G410), S433 (≠ G411), Y436 (= Y414), N458 (= N436), T460 (≠ G438), Y461 (= Y439), G462 (≠ R440)
Sites not aligning to the query:
- active site: 26, 28, 29, 30, 31, 50, 73, 112, 113, 114, 115, 163, 257, 284
- binding thiamine diphosphate: 26, 29, 50, 73
5dx6B Acetolactate synthase from klebsiella pneumoniae soaked with beta- fluoropyruvate
24% identity, 97% coverage: 2:492/506 of query aligns to 18:524/557 of 5dx6B
- active site: I38 (≠ N22), G40 (= G24), A41 (≠ T25), K42 (≠ S26), I43 (≠ E27), E63 (= E47), T86 (≠ H69), H125 (≠ P108), Q126 (≠ L109), S127 (≠ T110), Q175 (≠ S167), L268 (= L259), E295 (≠ D283), M392 (≠ A365), Q418 (≠ G384), M420 (≠ I386), D445 (= D409), D472 (≠ N436), G474 (= G438), Y475 (= Y439), M477 (≠ I441), V478 (≠ L442), Q481 (≠ E445)
- binding 3-fluoro-2-oxopropanoic acid: G264 (≠ A255), R265 (= R256), Q272 (≠ R263), A400 (= A373), R401 (= R374), Y404 (vs. gap)
- binding magnesium ion: S135 (≠ E118), T138 (≠ D121), D445 (= D409), D472 (≠ N436), G474 (= G438)
- binding thiamine diphosphate: G393 (≠ L366), S394 (≠ Y367), F395 (≠ E368), Q418 (≠ G384), M420 (≠ I386), G444 (≠ A408), D445 (= D409), G446 (= G410), D472 (≠ N436), G474 (= G438), Y475 (= Y439), N476 (≠ R440), M477 (≠ I441), V478 (≠ L442)
Sites not aligning to the query:
1ozgA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate (see paper)
24% identity, 97% coverage: 2:492/506 of query aligns to 7:521/549 of 1ozgA
- active site: I27 (≠ N22), G29 (= G24), A30 (≠ T25), K31 (≠ S26), I32 (≠ E27), E52 (= E47), T75 (≠ H69), H114 (≠ P108), Q115 (≠ L109), S116 (≠ T110), Q164 (≠ S167), L257 (= L259), E284 (≠ D283), M389 (≠ A365), Q415 (≠ G384), M417 (≠ I386), D442 (= D409), D469 (≠ N436), G471 (= G438), Y472 (= Y439), M474 (≠ I441), V475 (≠ L442), Q478 (≠ E445)
- binding 2-hydroxyethyl dihydrothiachrome diphosphate: M389 (≠ A365), G390 (≠ L366), S391 (≠ Y367), F392 (≠ E368), Q415 (≠ G384), M417 (≠ I386), G441 (≠ A408), D442 (= D409), G443 (= G410), D469 (≠ N436), G471 (= G438), Y472 (= Y439), N473 (≠ R440), M474 (≠ I441), V475 (≠ L442)
- binding magnesium ion: D442 (= D409), D469 (≠ N436), G471 (= G438)
- binding phosphate ion: G253 (≠ A255), R254 (= R256), Q261 (≠ R263), R347 (≠ D331), R398 (= R374), Y401 (vs. gap)
Sites not aligning to the query:
4rjkG Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
34% identity, 28% coverage: 3:145/506 of query aligns to 3:146/553 of 4rjkG
Sites not aligning to the query:
- binding magnesium ion: 437, 464, 466
- binding thiamine diphosphate: 384, 385, 386, 387, 410, 412, 436, 437, 438, 439, 466, 467, 468, 469, 470, 533
4rjiC Acetolactate synthase from bacillus subtilis bound to thdp - crystal form i (see paper)
34% identity, 28% coverage: 3:145/506 of query aligns to 4:147/555 of 4rjiC
Sites not aligning to the query:
- binding magnesium ion: 438, 465, 467
- binding thiamine diphosphate: 387, 388, 411, 437, 438, 439, 440, 467, 468, 469, 470, 471, 534
2ezuA Pyruvate oxidase variant f479w in complex with reaction intermediate 2-acetyl-thiamin diphosphate (see paper)
27% identity, 54% coverage: 11:284/506 of query aligns to 13:301/585 of 2ezuA
- active site: I24 (≠ N22), G26 (= G24), G27 (≠ T25), S28 (= S26), I29 (≠ E27), E51 (= E47), S74 (≠ H69), F113 (≠ P108), Q114 (≠ L109), E115 (≠ T110), V162 (= V158), R256 (= R247), E283 (vs. gap)
- binding flavin-adenine dinucleotide: H93 (= H88), G212 (vs. gap), I213 (≠ L203), G214 (= G204), T236 (= T231), Y237 (≠ F232), P238 (= P233), A254 (≠ I245), N255 (≠ E246), R256 (= R247), V257 (≠ L248), G276 (= G269), N277 (≠ T270), N278 (≠ D271), P280 (= P273), F281 (≠ S274), D298 (≠ E281), I299 (≠ Q282)
Sites not aligning to the query:
- active site: 386, 412, 414, 439, 466, 468, 469, 471, 472, 475, 538
- binding flavin-adenine dinucleotide: 303, 317, 318, 409
- binding 2-acetyl-thiamine diphosphate: 386, 388, 414, 438, 440, 466, 468, 469, 470, 471, 472
- binding magnesium ion: 439, 466, 468
- binding pyruvic acid: 547, 549, 550, 554
2ez9A Pyruvate oxidase variant f479w in complex with reaction intermediate analogue 2-phosphonolactyl-thiamin diphosphate (see paper)
27% identity, 54% coverage: 11:284/506 of query aligns to 13:301/585 of 2ez9A
- active site: I24 (≠ N22), G26 (= G24), G27 (≠ T25), S28 (= S26), I29 (≠ E27), E51 (= E47), S74 (≠ H69), F113 (≠ P108), Q114 (≠ L109), E115 (≠ T110), V162 (= V158), R256 (= R247), E283 (vs. gap)
- binding flavin-adenine dinucleotide: H93 (= H88), G212 (vs. gap), I213 (≠ L203), G214 (= G204), T236 (= T231), Y237 (≠ F232), P238 (= P233), A254 (≠ I245), N255 (≠ E246), R256 (= R247), V257 (≠ L248), G276 (= G269), N277 (≠ T270), N278 (≠ D271), P280 (= P273), F281 (≠ S274), D298 (≠ E281), I299 (≠ Q282)
Sites not aligning to the query:
- active site: 386, 412, 414, 439, 466, 468, 469, 471, 472, 475, 538
- binding flavin-adenine dinucleotide: 303, 317, 318, 409
- binding magnesium ion: 439, 466, 468
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(1s)-1-hydroxy-1-[(r)-hydroxy(methoxy)phosphoryl]ethyl}-5-(2-{[(s)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 386, 388, 414, 438, 440, 466, 468, 469, 470, 471, 472, 475
2ez8A Pyruvate oxidase variant f479w in complex with reaction intermediate 2-lactyl-thiamin diphosphate (see paper)
27% identity, 54% coverage: 11:284/506 of query aligns to 13:301/585 of 2ez8A
- active site: I24 (≠ N22), G26 (= G24), G27 (≠ T25), S28 (= S26), I29 (≠ E27), E51 (= E47), S74 (≠ H69), F113 (≠ P108), Q114 (≠ L109), E115 (≠ T110), V162 (= V158), R256 (= R247), E283 (vs. gap)
- binding flavin-adenine dinucleotide: H93 (= H88), G212 (vs. gap), I213 (≠ L203), G214 (= G204), T236 (= T231), Y237 (≠ F232), P238 (= P233), A254 (≠ I245), N255 (≠ E246), R256 (= R247), V257 (≠ L248), G276 (= G269), N277 (≠ T270), N278 (≠ D271), P280 (= P273), F281 (≠ S274), D298 (≠ E281), I299 (≠ Q282)
Sites not aligning to the query:
- active site: 386, 412, 414, 439, 466, 468, 469, 471, 472, 475, 538
- binding flavin-adenine dinucleotide: 303, 317, 318, 390, 409
- binding magnesium ion: 439, 466, 468
- binding pyruvic acid: 547, 549, 550, 553, 554
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 388, 414, 438, 440, 466, 468, 469, 470, 471, 472
2ez4B Pyruvate oxidase variant f479w (see paper)
27% identity, 54% coverage: 11:284/506 of query aligns to 13:301/585 of 2ez4B
- active site: I24 (≠ N22), G26 (= G24), G27 (≠ T25), S28 (= S26), I29 (≠ E27), E51 (= E47), S74 (≠ H69), F113 (≠ P108), Q114 (≠ L109), E115 (≠ T110), V162 (= V158), R256 (= R247), E283 (vs. gap)
- binding flavin-adenine dinucleotide: H93 (= H88), G212 (vs. gap), I213 (≠ L203), G214 (= G204), T236 (= T231), Y237 (≠ F232), P238 (= P233), A254 (≠ I245), N255 (≠ E246), R256 (= R247), V257 (≠ L248), G276 (= G269), N277 (≠ T270), N278 (≠ D271), P280 (= P273), F281 (≠ S274), D298 (≠ E281), I299 (≠ Q282)
Sites not aligning to the query:
- active site: 386, 412, 414, 439, 466, 468, 469, 471, 472, 475, 538
- binding flavin-adenine dinucleotide: 303, 317, 318, 409
- binding magnesium ion: 439, 466, 468
- binding phosphate ion: 471, 475
- binding thiamine diphosphate: 388, 412, 414, 438, 439, 440, 441, 466, 468, 469, 470, 471, 472
4rjkF Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
34% identity, 28% coverage: 3:145/506 of query aligns to 3:146/552 of 4rjkF
Sites not aligning to the query:
- binding magnesium ion: 437, 464, 466
- binding thiamine diphosphate: 385, 386, 387, 410, 412, 436, 437, 438, 439, 466, 467, 468, 469, 470, 533
1powA The refined structures of a stabilized mutant and of wild-type pyruvate oxidase from lactobacillus plantarum (see paper)
27% identity, 55% coverage: 11:287/506 of query aligns to 13:294/585 of 1powA
- active site: I24 (≠ N22), G26 (= G24), G27 (≠ T25), S28 (= S26), I29 (≠ E27), E51 (= E47), S74 (≠ H69), F113 (≠ P108), Q114 (≠ L109), E115 (≠ T110), V162 (= V158), R256 (= R247), E283 (≠ W276)
- binding flavin-adenine dinucleotide: H93 (= H88), G212 (vs. gap), I213 (≠ L203), G214 (= G204), T236 (= T231), Y237 (≠ F232), A254 (≠ I245), V257 (≠ L248), G276 (= G269), N277 (≠ T270), N278 (≠ D271), Y279 (≠ A272), P280 (= P273), F281 (≠ S274)
Sites not aligning to the query:
- active site: 386, 412, 414, 439, 466, 468, 469, 471, 472, 475, 538
- binding flavin-adenine dinucleotide: 298, 299, 303, 317, 318, 409
- binding magnesium ion: 439, 466, 468
- binding thiamine diphosphate: 388, 414, 440, 466, 468, 469, 470, 471, 472
4feeA High-resolution structure of pyruvate oxidase in complex with reaction intermediate 2-hydroxyethyl-thiamin diphosphate carbanion-enamine, crystal b (see paper)
27% identity, 55% coverage: 11:287/506 of query aligns to 13:294/586 of 4feeA
- binding flavin-adenine dinucleotide: H93 (= H88), G212 (vs. gap), I213 (≠ L203), G214 (= G204), T236 (= T231), Y237 (≠ F232), P238 (= P233), A254 (≠ I245), N255 (≠ E246), V257 (≠ L248), G276 (= G269), N277 (≠ T270), N278 (≠ D271), P280 (= P273), F281 (≠ S274)
- binding pyruvic acid: N255 (≠ E246), R256 (= R247)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 298, 299, 303, 317, 318, 390, 409
- binding magnesium ion: 439, 466, 468
- binding pyruvic acid: 547, 549, 550, 553, 554
- binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: 386, 388, 412, 414, 438, 440, 466, 468, 469, 470, 471, 472
1ozfA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors (see paper)
25% identity, 97% coverage: 2:492/506 of query aligns to 6:517/545 of 1ozfA
- active site: I26 (≠ N22), G28 (= G24), A29 (≠ T25), K30 (≠ S26), I31 (≠ E27), E51 (= E47), T74 (≠ H69), H113 (≠ P108), Q114 (≠ L109), S115 (≠ T110), Q163 (≠ S167), L253 (= L259), E280 (≠ D283), M385 (≠ A365), Q411 (≠ G384), M413 (≠ I386), D438 (= D409), D465 (≠ N436), G467 (= G438), Y468 (= Y439), M470 (≠ I441), V471 (≠ L442), Q474 (≠ E445)
- binding magnesium ion: D438 (= D409), D465 (≠ N436), G467 (= G438)
- binding phosphate ion: G249 (≠ A255), R250 (= R256), Q257 (≠ R263), R343 (≠ D331), R394 (= R374), L396 (vs. gap), Y397 (vs. gap)
- binding thiamine diphosphate: G386 (≠ L366), S387 (≠ Y367), F388 (≠ E368), Q411 (≠ G384), M413 (≠ I386), G437 (≠ A408), D438 (= D409), G439 (= G410), D465 (≠ N436), G467 (= G438), Y468 (= Y439), N469 (≠ R440), M470 (≠ I441), V471 (≠ L442)
Sites not aligning to the query:
6a50A Structure of benzoylformate decarboxylases in complex with cofactor tpp
26% identity, 30% coverage: 354:505/506 of query aligns to 371:525/527 of 6a50A
- binding magnesium ion: D428 (= D409), N455 (= N436), T457 (≠ G438)
- binding thiamine diphosphate: T377 (≠ R360), S378 (≠ T361), G401 (= G384), L403 (≠ I386), G427 (≠ A408), D428 (= D409), G429 (= G410), S430 (≠ G411), Y433 (= Y414), N455 (= N436), T457 (≠ G438), Y458 (= Y439), G459 (≠ R440), M460 (≠ I441), L461 (= L442)
Sites not aligning to the query:
1y9dD Pyruvate oxidase variant v265a from lactobacillus plantarum (see paper)
27% identity, 54% coverage: 11:284/506 of query aligns to 13:276/560 of 1y9dD
- active site: I24 (≠ N22), G26 (= G24), G27 (≠ T25), S28 (= S26), I29 (≠ E27), E51 (= E47), S74 (≠ H69), E108 (≠ R103), V155 (= V158), R241 (= R247)
- binding flavin-adenine dinucleotide: I198 (≠ L203), G199 (= G204), T221 (= T231), P223 (= P233), G261 (= G269), N262 (≠ T270), N263 (≠ D271), D273 (≠ E281), I274 (≠ Q282)
- binding thiamine diphosphate: E51 (= E47), S74 (≠ H69), P77 (= P72), H81 (≠ N76)
Sites not aligning to the query:
- active site: 361, 387, 389, 414, 441, 443, 444, 446, 447, 450, 513
- binding flavin-adenine dinucleotide: 278, 292, 293
- binding magnesium ion: 414, 441, 443
- binding thiamine diphosphate: 363, 389, 413, 415, 441, 443, 444, 445, 446, 447
3f6bX Crystal structure of benzoylformate decarboxylase in complex with the pyridyl inhibitor paa (see paper)
26% identity, 30% coverage: 354:505/506 of query aligns to 370:524/525 of 3f6bX
Sites not aligning to the query:
- active site: 24, 46, 108, 109, 110, 111
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-2-[(1S,2E)-1-hydroxy-3-pyridin-3-ylprop-2-en-1-yl]-4-methyl-1,3-thiazol-3-ium: 22, 23, 24, 25, 46, 69
5deiA Benzoylformate decarboxylase from pseudomonas putida
26% identity, 30% coverage: 354:505/506 of query aligns to 370:524/524 of 5deiA
- active site: S375 (≠ A359), G400 (= G384), L402 (≠ I386), D427 (= D409), N454 (= N436), T456 (≠ G438), Y457 (= Y439), A459 (≠ I441), L460 (= L442), F463 (≠ I448), S524 (= S505)
- binding calcium ion: D427 (= D409), N454 (= N436), T456 (≠ G438)
- binding thiamine diphosphate: T376 (≠ R360), S377 (≠ T361), L402 (≠ I386), G426 (≠ A408), D427 (= D409), G428 (= G410), S429 (≠ G411), Y432 (= Y414), T456 (≠ G438), Y457 (= Y439), G458 (≠ R440), A459 (≠ I441), L460 (= L442)
Sites not aligning to the query:
- active site: 22, 24, 25, 26, 27, 46, 69, 108, 109, 110, 111, 159, 253, 280
- binding bicarbonate ion: 25, 69, 109
- binding calcium ion: 185, 186
- binding magnesium ion: 116, 117, 119
- binding thiamine diphosphate: 22, 23, 46, 69
3fsjX Crystal structure of benzoylformate decarboxylase in complex with the inhibitor mbp (see paper)
26% identity, 30% coverage: 354:505/506 of query aligns to 370:524/524 of 3fsjX
- active site: G400 (= G384), D427 (= D409), N454 (= N436), T456 (≠ G438), Y457 (= Y439), A459 (≠ I441), L460 (= L442), S524 (= S505)
- binding calcium ion: D427 (= D409), N454 (= N436), T456 (≠ G438)
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(S)-hydroxy[(R)-hydroxy(methoxy)phosphoryl]phenylmethyl}-5-(2-{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: T376 (≠ R360), S377 (≠ T361), F396 (≠ G380), G400 (= G384), L402 (≠ I386), G426 (≠ A408), D427 (= D409), G428 (= G410), S429 (≠ G411), Y432 (= Y414), T456 (≠ G438), Y457 (= Y439), G458 (≠ R440), A459 (≠ I441), L460 (= L442), F463 (≠ I448), V523 (= V504), S524 (= S505)
Sites not aligning to the query:
- active site: 24, 25, 27, 46, 69, 108, 109, 110, 111, 280
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(S)-hydroxy[(R)-hydroxy(methoxy)phosphoryl]phenylmethyl}-5-(2-{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 280
Query Sequence
>WP_015799002.1 NCBI__GCF_000023265.1:WP_015799002.1
MNGAELAALTLSDLGVGVVFANPGTSELVLVDALERRGPRPILVTHELVAMGAADAHGRL
GRLGAALVHLGPGLSNGLAFVHDARRAHTPMLVLVGEHPRSHRALDTPLTMRIASLAEAI
DVPMIAIDDPALIAPQLRAAATIATTQRTPVVVTLDAVAMEREATPSDPSAPPTTAHEAP
SIPRARLEAAIDLVRAGECTLLLGARVLEPDARALARAIADDTGLRLLAETFPASHRYGE
GAGRIERLAYLPLMARPQLATARSVLVLGTDAPSAWFAHLEQDPRLWPLAAPLVRLDGQD
EDPRVVLEHLAKALGVTPAPPPTSQPIPAPDPHAALDGSTLAAVIARAVQADDVVIDEAR
TAAPALYEQLADARRHHYIGHPGGAIGEGASLALGAATLGASVLAVVADGGFLYAPQAIW
TMVREHLDVTVVLAVNGGYRILEIEQTIAGLDLHPELTRLDDPGWDFAGLAAAMGARTTT
ATTVAELEAALRDRHGVHVIVATVSQ
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory