SitesBLAST

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
38% identity, 98% coverage: 3:182/184 of query aligns to 2:181/183 of 3nz2C

query
sites
3nz2C

S

S

E

E

L

L

E

E

K

K

M

M

V

L

A

K

G

G

E

E

P

H

Y

F

D

D

A

G

H

A

C

S

E

A

E

E

M

I

L

E

E

A

I

L

R

R

T

S

R

Q

V

A

K

G

R

R

I

L

L

K

H

L

K

E

L

I

N

N

V

Q

T

S

E

L

Y

D

Y

E

T

A

E

E

N

R

F

Y

K

-

A

A

V

L

T

Q

H

R

E

E

L

L

C

F

P

G

N

H

S

L

A

G

K

H

D

K

L

S

H

C

L

V

E

Q

P

P

F

F

Y

H

C

C

D

E

Y

F

G

G

E

K

N

T

I

I

H

R

A

I

A

G

E

D

G

H

V

T

F

F

I

I

N

N

F

M

G

N

A

V

V

V

I

M

L

L

D

D

G

G

S

A

T

P

V

I

T

T

I

I

G

G

K

D

K

H

T

V

L

L

I

I

A

G

P

P

G

S

V

T

H

Q

I

F

Y
|
Y

T

T

N

A

R

S

H
|
H

P

S

V

L

E

D

V

Y

K

R

E

R

R

R

R

Q

E

A

W

W

E

E

D

T

-

I

C

C

A

K

P

P

V

I

T

V

I

I

G

E

E

D

C

V

W

W

I

I

G
|
G

G

G

H

N

S

V

T

V

I

I

C

N

P

Q

G

G

V

V

T

T

I

I

G

G

D

A

R

R

A

S

V

V

I

V

G
x
A

A
|
A

G
x
N

S

S

V

V

V
x
N

K

Q

D

D

I

V

P

P

A

P

D

D

S

T

L

L

A

V

V
x
G

G

G

N
x
T

P
|
P

A

A

K

R

V

I

V
x
L

K
x
R

K

S

L

L

binding acetyl coenzyme *a: Y107 (= Y109), H111 (= H113), G137 (= G138), A155 (≠ G156), A156 (= A157), N161 (≠ V162), G171 (≠ V172), T173 (≠ N174), P174 (= P175), L178 (≠ V179), R179 (≠ K180)
binding magnesium ion: N157 (≠ G158), T173 (≠ N174)

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
38% identity, 98% coverage: 3:182/184 of query aligns to 5:184/185 of 3nz2J

query
sites
3nz2J

S

S

E

E

L

L

E

E

K

K

M

M

V

L

A

K

G

G

E

E

P

H

Y

F

D

D

A

G

H

A

C

S

E

A

E

E

M

I

L

E

E

A

I

L

R

R

T

S

R

Q

V

A

K

G

R

R

I

L

L

K

H

L

K

E

L

I

N

N

V

Q

T

S

E

L

Y

D

Y

E

T

A

E

E

N

R

F

Y

K

-

A

A

V

L

T

Q

H

R

E

E

L

L

C

F

P

G

N

H

S

L

A

G

K

H

D

K

L

S

H

C

L

V

E

Q

P

P

F

F

Y

H

C

C

D

E

Y

F

G

G

E

K

N

T

I

I

H

R

A

I

A

G

E

D

G

H

V

T

F

F

I

I

N

N

F

M

G

N

A

V

V

V

I

M

L

L

D

D

G

G

S

A

T

P

V

I

T

T

I

I

G

G

K

D

K

H

T

V

L

L

I

I

A
x
G

P

P

G

S

V

T

H

Q

I

F

Y
|
Y

T

T

N

A

R

S

H
|
H

P

S

V

L

E

D

V

Y

K

R

E

R

R

R

R

Q

E

A

W

W

E

E

D

T

-

I

C

C

A

K

P

P

V

I

T

V

I

I

G

E

E

D

C

V

W
|
W

I

I

G
|
G

G

G

H

N

S

V

T

V

I

I

C

N

P

Q

G

G

V

V

T

T

I

I

G

G

D

A

R

R

A

S

V

V

I

V

G
x
A

A
|
A

G

N

S

S

V

V

V
x
N

K

Q

D

D

I

V

P

P

A

P

D

D

S

T

L

L

A

V

V
x
G

G

G

N
x
T

P
|
P

A

A

K

R

V

I

V

L

K
x
R

K

S

L

L

binding acetyl coenzyme *a: G104 (≠ A103), Y110 (= Y109), H114 (= H113), W138 (= W136), G140 (= G138), A158 (≠ G156), A159 (= A157), N164 (≠ V162), G174 (≠ V172), T176 (≠ N174), P177 (= P175), R182 (≠ K180)

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
41% identity, 99% coverage: 3:184/184 of query aligns to 2:186/190 of 5u2kA

query
sites
5u2kA

S

T

E

E

L

K

E

E

K

K

M

M

V

L

A

A

G

E

E

K

P

W

Y

Y

D

D

A

A

H

N

C

F

E

D

E

Q

-

Y

M

L

L

I

E

N

I

E

R

R

T

A

R

R

V

A

K

K

R

D

I

I

L

C

H

F

K

E

L

L

N

N

V

H

T

T

E

R

-

P

Y

S

Y

A

T

T

E

N

N

K

F

R

K

K

A

E

V

L

T

I

H

D

E

Q

L

L

C

F

P

Q

N

T

S

T

A

T

K

D

D

N

L

V

H

S

L

I

E

S

P

I

P

P

F

F

Y

D

C

T

D

D

Y

Y

G

G

E

W

N

N

I

V

H

K

A

L

A

G

E

K

G

N

V

V

F

Y

I

V

N

N

F

T

G

N

A

C

V

Y

I

F

L
x
M

D

D

G

G

S

G

T

Q

V

I

T

T

I

I

G

G

K

D

K

N

T

V

L

F

I

I

A

G

P

P

G

N

V

C

H

G

I

F

Y
|
Y

T

T

N
x
A

R

T

H
|
H

P

P

V

L

E

N

V

F

K

H

E

H

R

R

R

N

E

E

-

G

W

F

E

E

D

K

C

A

A

G

P

P

V

I

T

H

I

I

G

G

E

S

E

N

C

T

W

W

I

F

G

G

H

H

S

V

T

A

I

V

C
x
L

P

P

G

G

V

V

T

T

I

I

G

G

D

E

R

G

A

S

V

V

I

I

G

G

A

A

G

G

S

S

V

V

V
x
T

K
|
K

D

D

I

I

P

P

A

P

D

H

S

S

L

L

A

A

V

V

G

G

N

N

P

P

A

C

K

K

V

V

K

R

K

K

L

I

N

D

Q

N

binding coenzyme a: M90 (≠ L89), Y110 (= Y109), A112 (≠ N111), H114 (= H113), L146 (≠ C144), T164 (≠ V162), K165 (= K163)

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
40% identity, 97% coverage: 6:184/184 of query aligns to 6:186/200 of 1krrA

query
sites
1krrA

E

E

K

R

M

I

V

R

A

A

G

G

E

K

P

L

Y

F

D

T

A

D

H

M

C

C

E

E

E

G

M

L

L

P

E

E

I

K

R

R

T

L

R

R

V

G

K

K

R

T

I

L

L

M

H

Y

K

E

L

F

N

N

V

H

T

S

E

H

-

P

Y

S

Y

E

T

V

E

E

N

K

F

R

K

E

A

S

V

L

T

I

H

K

E

E

L

M

C

F

P

A

N

T

S

V

A

G

K

E

D

N

L

A

H

W

L

V

E

E

P

P

F

V

Y

Y

C

F

D

S

Y

Y

G

G

E

S

N

N

I

I

H

H

A

I

A

G

E

R

G

N

V

F

F

Y

I

A

N
|
N

F

F

G

N

A

L

V

T

I

I

L

V

D

D

G

D

S

Y

T

T

V

V

T

T

I

I

G

G

K

D

K

N

T

V

L

L

I
|
I

A
|
A

P
|
P

G

N

V

V

H

T

I

L

Y

S

T

V

N
x
T

R

G

H
|
H

P

P

V

V

E

H

V

H

K

E

E

L

R

R

R

K

E

N

W

G

E

E

D

M

C

Y

A

S

-

F

P

P

V

I

T

T

I

I

G

G

E

N

C

V

W

W

I

I

G
|
G

G
x
S

H

H

S

V

T

V

I

I

C
x
N

P

P

G

G

V

V

T

T

I

I

G

G

D

D

R

N

A

S

V

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

V

T

K

K

D

D

I

I

P

P

A

P

D

N

S

V

L

V

A

A

V
x
A

G

G

N

V

P
|
P

A

C

K

R

V

V

V

I

K
x
R

K

E

L

I

N

N

Q

D

binding acetyl coenzyme *a: N84 (= N83), I103 (= I102), A104 (= A103), P105 (= P104), T112 (≠ N111), H114 (= H113), G140 (= G138), S141 (≠ G139), N146 (≠ C144), G158 (= G156), A159 (= A157), A174 (≠ V172), P177 (= P175), R182 (≠ K180)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
40% identity, 97% coverage: 6:184/184 of query aligns to 7:187/203 of P07464

query
sites
P07464

E

E

K

R

M

I

V

R

A

A

G

G

E

K

P

L

Y

F

D

T

A
x
D

H

M

C

C

E

E

E

G

M

L

L

P

E

E

I

K

R

R

T

L

R

R

V

G

K

K

R

T

I

L

L

M

H

Y

K

E

L

F

N

N

V

H

T

S

E

H

-

P

Y

S

Y

E

T

V

E

E

N

K

F

R

K

E

A

S

V

L

T

I

H

K

E

E

L

M

C

F

P

A

N

T

S

V

A

G

K

E

D

N

L

A

H

W

L

V

E

E

P

P

F

V

Y

Y

C

F

D
x
S

Y

Y

G

G

E

S

N

N

I

I

H

H

A

I

A

G

E

R

G

N

V

F

F

Y

I

A

N
|
N

F

F

G

N

A

L

V

T

I

I

L

V

D

D

G
x
D

S

Y

T

T

V

V

T

T

I

I

G

G

K

D

K

N

T

V

L

L

I

I

A

A

P

P

G

N

V

V

H

T

I

L

Y

S

T

V

N

T

R

G

H
|
H

P

P

V

V

E

H

V

H

K

E

E

L

R

R

R

K

E

N

W

G

E

E

D

M

C

Y

A

S

-

F

P

P

V

I

T

T

I

I

G

G

E

N

C

V

W

W

I

I

G

G

G
x
S

H

H

S

V

T

V

I

I

C

N

P

P

G

G

V

V

T

T

I

I

G

G

D

D

R

N

A

S

V

V

I

I

G

G

A
|
A

G

G

S

S

V

I

V

V

V
x
T

K
|
K

D

D

I

I

P

P

A

P

D

N

S

V

L

V

A

A

V

A

G

G

N

V

P

P

A

C

K
x
R

V

V

V

I

K
x
R

K

E

L

I

N

N

Q

D

D17 (≠ A16) binding substrate
S71 (≠ D69) binding substrate
N85 (= N83) binding in other chain; binding in other chain
D93 (≠ G91) binding substrate
H115 (= H113) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ G139) binding in other chain
A160 (= A157) binding in other chain
TK 165:166 (≠ VK 162:163) binding acetyl-CoA
R180 (≠ K177) binding acetyl-CoA
R183 (≠ K180) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
40% identity, 97% coverage: 6:184/184 of query aligns to 6:186/201 of 1krvA

query
sites
1krvA

E

E

K

R

M

I

V

R

A

A

G

G

E

K

P

L

Y

F

D

T

A
x
D

H

M

C

C

E

E

E

G

M

L

L

P

E

E

I

K

R
|
R

T

L

R

R

V

G

K

K

R

T

I

L

L

M

H

Y

K

E

L

F

N

N

V

H

T

S

E

H

-

P

Y

S

Y

E

T

V

E

E

N

K

F

R

K

E

A

S

V

L

T

I

H

K

E

E

L

M

C

F

P

A

N

T

S

V

A

G

K

E

D

N

L

A

H

W

L

V

E

E

P

P

F

V

Y

Y

C

F

D
x
S

Y

Y

G

G

E

S

N

N

I

I

H

H

A

I

A

G

E

R

G

N

V

F

F
x
Y

I

A

N
|
N

F

F

G

N

A

L

V

T

I

I

L
x
V

D

D

G
x
D

S

Y

T

T

V

V

T

T

I

I

G

G

K

D

K

N

T

V

L

L

I

I

A
|
A

P

P

G

N

V

V

H

T

I

L

Y
x
S

T

V

N
x
T

R

G

H

H

P

P

V

V

E

H

V

H

K

E

E

L

R

R

R

K

E

N

W

G

E

E

D
x
M

C

Y

A

S

-

F

P

P

V

I

T

T

I

I

G

G

E

N

C

V

W
|
W

I

I

G
|
G

G
x
S

H

H

S

V

T

V

I

I

C
x
N

P

P

G

G

V

V

T

T

I

I

G

G

D

D

R

N

A

S

V

V

I

I

G

G

A
|
A

G

G

S

S

V

I

V

V

V

T

K

K

D

D

I

I

P

P

A

P

D

N

S

V

L

V

A

A

V

A

G

G

N

V

P
|
P

A

C

K
x
R

V

V

V

I

K
x
R

K

E

L

I

N

N

Q

D

binding 4-nitrophenyl beta-D-galactopyranoside: D16 (≠ A16), R25 (= R25), S70 (≠ D69), Y82 (≠ F81), N84 (= N83), V90 (≠ L89), D92 (≠ G91), M126 (≠ D125)
binding coenzyme a: A104 (= A103), S110 (≠ Y109), T112 (≠ N111), W138 (= W136), G140 (= G138), S141 (≠ G139), N146 (≠ C144), A159 (= A157), P177 (= P175), R179 (≠ K177), R182 (≠ K180)

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
40% identity, 97% coverage: 6:184/184 of query aligns to 6:186/201 of 1kruA

query
sites
1kruA

E

E

K

R

M

I

V

R

A

A

G

G

E

K

P

L

Y

F

D

T

A
x
D

H

M

C

C

E

E

E

G

M

L

L
x
P

E

E

I

K

R
|
R

T

L

R

R

V

G

K

K

R

T

I

L

L

M

H

Y

K

E

L

F

N

N

V

H

T

S

E

H

-

P

Y

S

Y

E

T

V

E

E

N

K

F

R

K

E

A

S

V

L

T

I

H

K

E

E

L

M

C

F

P

A

N

T

S

V

A

G

K

E

D

N

L

A

H
x
W

L

V

E

E

P

P

F

V

Y

Y

C

F

D
x
S

Y

Y

G

G

E

S

N

N

I

I

H

H

A

I

A

G

E

R

G

N

V

F

F
x
Y

I

A

N
|
N

F

F

G

N

A

L

V

T

I

I

L
x
V

D

D

G
x
D

S

Y

T

T

V

V

T

T

I

I

G

G

K

D

K

N

T

V

L
|
L

I

I

A

A

P

P

G

N

V

V

H

T

I

L

Y

S

T

V

N

T

R

G

H
|
H

P

P

V

V

E

H

V

H

K

E

E

L

R

R

R

K

E

N

W

G

E

E

D
x
M

C

Y

A

S

-

F

P

P

V

I

T

T

I

I

G

G

E

N

C

V

W
|
W

I

I

G
|
G

G
x
S

H

H

S

V

T

V

I

I

C

N

P

P

G

G

V

V

T

T

I

I

G

G

D

D

R

N

A

S

V

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

V

T

K

K

D

D

I

I

P

P

A

P

D

N

S

V

L

V

A

A

V

A

G

G

N

V

P
|
P

A

C

K

R

V

V

V

I

K
x
R

K

E

L

I

N

N

Q

D

binding coenzyme a: H114 (= H113), W138 (= W136), G140 (= G138), S141 (≠ G139), G158 (= G156), A159 (= A157), P177 (= P175), R182 (≠ K180)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: D16 (≠ A16), P22 (≠ L22), R25 (= R25), W62 (≠ H61), S70 (≠ D69), Y82 (≠ F81), Y82 (≠ F81), N84 (= N83), V90 (≠ L89), D92 (≠ G91), L102 (= L101), H114 (= H113), M126 (≠ D125)

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
36% identity, 97% coverage: 5:182/184 of query aligns to 1:174/176 of 3ectA

query
sites
3ectA

L

L

E

E

K

K

M

M

V

L

A

K

G

G

E

H

P

A

Y

-

D

-

A

-

H

-

C

S

E

A

E

E

M

I

L

E

E

A

I

L

R

R

T

S

R

Q

V

A

K

G

R

R

I

L

L

K

H

L

K

E

L

I

N

N

V

Q

T

S

E

L

Y

D

Y

E

T

A

E

E

N

R

F

Y

K

-

A

A

V

L

T

Q

H

R

E

E

L

L

C

F

P

G

N

H

S

L

A

G

K

H

D

K

L

S

H

C

L

V

E

Q

P

P

F

F

Y

H

C

C

D

E

Y

F

G

G

E

K

N

T

I

I

H

R

A

I

A

G

E

D

G

H

V

T

F

F

I

I

N

N

F

M

G

N

A

V

V

V

I

M

L

L

D

D

G

G

S

A

T

P

V

I

T

T

I

I

G

G

K

D

K

H

T

V

L

L

I

I

A

G

P

P

G

S

V

T

H

Q

I

F

Y

Y

T

T

N

A

R

S

H

H

P

S

V

L

E

D

V

Y

K

R

E

R

R

R

R

Q

E

A

W

W

E

E

D

T

-

I

C

C

A

K

P

P

V

I

T

V

I

I

G

E

E

D

C

V

W

W

I

I

G

G

H

N

S

V

T

V

I

I

C

N

P

Q

G
|
G

V

V

T

T

I

I

G

G

D

A

R

R

A

S

V

V

I

V

G

A

A

A

G

N

S

S

V

V

V

N

K

Q

D
|
D

I

V

P

P

A

P

D

D

S

T

L

L

A

V

V

G

G

G

N

T

P

P

A

A

K

R

V

I

V

L

K

R

K

S

L

L

binding calcium ion: G138 (= G146), D156 (= D164)

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
37% identity, 52% coverage: 87:181/184 of query aligns to 183:279/307 of A1ADJ6

query
sites
A1ADJ6

V

V

I

V

L

T

D

D

G

K

S

C

T

N

V

V

T

T

I

I

G

G

K

H

K

D

T

C

L

M

I

I

A

A

P

R

G

D

V

V

H

I

I

L

Y

R

-

A

T

S

N

D

R

G

H
|
H

P

P

V

I

-

F

-

D

-

I

E

H

V

S

K

K

E

K

R

R

R

I

E

N

W

W

E

-

D

-

C

A

A

K

P

D

V

I

T

I

I

I

G

S

E

S

E

Y

C

V

W
|
W

I

V

G

G

G

R

H

N

S

V

T

S

I

I

C

M

P

K

G

G

V

V

T

S

I

V

G

G

D

S

R

G

A

S

V

V

I

I

G

G

A

Y

G

G

S

S

V

I

V

V

V

T

K

K

D

D

I

V

P

P

A

S

D

M

S

C

L

A

A

A

V

A

G

G

N

N

P

P

A

A

K

K

V

I

K

K

K

R

H210 (= H113) mutation to A: Loss of activity.
W234 (= W136) mutation to A: Loss of activity.

8vr7E Crystal structure of the pcryo_0619 n-acetyltransferase from psychrobacter cryohalolentis k5 int he presence of acetyl coenzyme a
42% identity, 42% coverage: 102:179/184 of query aligns to 91:169/180 of 8vr7E

query
sites
8vr7E

I

I

A
x
S

P

A

G

G

V

V

H

Q

I

I

Y
|
Y

T

T

N

-

R

-

H

H

P
x
D

V
x
T

E

V

V

R

K

K

E

S

R

L

R

S

E

G

W

G

E

K

-

A

-

D

-

I

D

D

C

K

A

A

P

S

V

T

T

R

I

I

G

G

E

S

E

D

C

C

W
x
Y

I

L

G

G

G

P

H

N

S

T

T

I

I

I

C
x
V

P

K

G

G

V

V

T

K

I

I

G

G

D

D

R

R

A

V

V

V

I

V

G
|
G

A
|
A

G

N

S

S

V
x
L

V

V

V
x
L

K

K

D

D

I

I

P

P

A

S

D

D

S

C

L
x
K

A

V

V
x
F

G

G

N
x
S

P
|
P

A

A

K

V

V

I

binding acetyl coenzyme *a: S92 (≠ A103), Y98 (= Y109), D101 (≠ P114), T102 (≠ V115), Y126 (≠ W136), V134 (≠ C144), G146 (= G156), A147 (= A157), L150 (≠ V160), L152 (≠ V162), K160 (≠ L170), F162 (≠ V172), S164 (≠ N174), P165 (= P175)

Sites not aligning to the query:

8vr6A Crystal structure of the pcryo_0619 n-acetryltransferase from psychrobacter cryohalolentis k5 in the presence of coa-disulfide
42% identity, 42% coverage: 102:179/184 of query aligns to 90:168/177 of 8vr6A

query
sites
8vr6A

I

I

A

S

P

A

G

G

V

V

H

Q

I

I

Y
|
Y

T

T

N

-

R

-

H
|
H

P
x
D

V
x
T

E

V

V

R

K

K

E

S

R

L

R

S

E

G

W

G

E

K

-

A

-

D

-

I

D

D

C

K

A

A

P

S

V

T

T

R

I

I

G

G

E

S

E

D

C

C

W

Y

I

L

G

G

G

P

H

N

S

T

T

I

I

I

C
x
V

P

K

G

G

V

V

T

K

I

I

G

G

D

D

R

R

A

V

V

V

I

V

G

G

A

A

G

N

S

S

V
x
L

V

V

V
x
L

K

K

D

D

I

I

P

P

A

S

D

D

S

C

L

K

A

V

V

F

G

G

N
x
S

P
|
P

A

A

K

V

V

I

binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: Y97 (= Y109), H99 (= H113), D100 (≠ P114), T101 (≠ V115), V133 (≠ C144), L149 (≠ V160), L151 (≠ V162), S163 (≠ N174), P164 (= P175)

8vr7B Crystal structure of the pcryo_0619 n-acetyltransferase from psychrobacter cryohalolentis k5 int he presence of acetyl coenzyme a
42% identity, 42% coverage: 102:179/184 of query aligns to 91:169/177 of 8vr7B

query
sites
8vr7B

I

I

A

S

P

A

G

G

V

V

H

Q

I

I

Y
|
Y

T

T

N

-

R

-

H

H

P
x
D

V
x
T

E

V

V

R

K

K

E

S

R

L

R

S

E

G

W

G

E

K

-

A

-

D

-

I

D

D

C

K

A

A

P

S

V

T

T
x
R

I

I

G

G

E

S

E

D

C

C

W
x
Y

I

L

G

G

G
x
P

H

N

S

T

T

I

I

I

C

V

P

K

G

G

V

V

T

K

I

I

G

G

D

D

R

R

A

V

V

V

I

V

G
|
G

A
|
A

G

N

S

S

V
x
L

V

V

V
x
L

K

K

D

D

I

I

P

P

A

S

D

D

S

C

L
x
K

A

V

V
x
F

G

G

N

S

P
|
P

A

A

K

V

V

I

binding acetyl coenzyme *a: Y98 (= Y109), D101 (≠ P114), T102 (≠ V115), L150 (≠ V160), L152 (≠ V162), P165 (= P175)
binding coenzyme a: Y126 (≠ W136), P129 (≠ G139), G146 (= G156), A147 (= A157), K160 (≠ L170), F162 (≠ V172)
binding 3'-phosphate-adenosine-5'-diphosphate: R120 (≠ T130)

Sites not aligning to the query:

8j40A Crystal structure of catb8 in complex with chloramphenicol (see paper)
37% identity, 41% coverage: 107:181/184 of query aligns to 88:162/209 of 8j40A

query
sites
8j40A

H

F

I

F

Y
|
Y

T

M

N

Q

R

E

H

E

P

P

V

A

E

F

V

S

K

R

E

A

R

L

R

D

E

A

W

F

E

Q

D

R

C

A

A

G

P

D

V

T

T

V

I

I

G

G

E

N

E

D

C

V

W
|
W

I

I

G

G

G

S

H

E

S

A

T

M

I

I

C

M

P

P

G

G

V

I

T

K

I

I

G

G

D

D

R

G

A

A

V
|
V

I

I

G

G

A
x
S

G

R

S

S

V

L

V

V

V

T

K

K

D

D

I

V

P

E

A

P

D

Y

S

A

L

I

A

I

V

G

G

G

N

N

P

P

A

A

K

K

V

Q

V

I

K

K

binding chloramphenicol: Y90 (= Y109), W117 (= W136), V135 (= V154), S138 (≠ A157)

Sites not aligning to the query:

binding chloramphenicol: 7, 8, 9, 29, 31, 47, 50, 187, 190, 191, 202, 203, 206

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
30% identity, 55% coverage: 77:178/184 of query aligns to 50:139/290 of 4mzuB

query
sites
4mzuB

A

G

E

D

G

N

V

V

F

T

I

I

N

K

F

S

G
|
G

A

V

V

Q

I

I

L

W

D

D

G

G

S

-

T

-

V

I

T

H

I

I

G

Q

K

D

T

V

L
x
F

I

I

A

G

P

P

G
x
N

V

V

H

T

I

F

Y
x
T

T
x
N

N
x
D

R
x
K

H

Q

P
|
P

V

R

E

S

V

L

K

K

E

-

R

-

E

-

W

-

E

-

D

-

C

-

A

-

P

-

V

T

T

I

I

V

G

K

E

K

E

G

C

A

W

S

I

I

G
|
G

G
x
A

H

N

S

S

T

T

I

I

C

L

P

P

G

G

V

I

T

L

I

I

G

G

D

E

R

N

A

A

V

M

I

V

G
|
G

A
|
A

G

G

S

A

V

V

V

I

V
x
T

K
|
K

D

N

I

V

P

P

A

D

D

N

S

A

L

I

A

V

V
x
I

G

G

N

N

P

P

A

G

K
x
R

V

I

binding coenzyme a: F72 (≠ L101), T80 (≠ Y109), N81 (≠ T110), D82 (≠ N111), K83 (≠ R112), P85 (= P114), G99 (= G138), A100 (≠ G139), G117 (= G156), A118 (= A157), T123 (≠ V162), K124 (= K163), I133 (≠ V172), R138 (≠ K177)
binding magnesium ion: G58 (= G85), N76 (≠ G105)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymine: 20, 21, 38, 39
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
38% identity, 48% coverage: 94:181/184 of query aligns to 61:166/203 of 3dhoA

query
sites
3dhoA

V

L

T

K

I

I

G

G

K

K

K

F

T

C

L

S

I

I

A

G

P

P

G

G

V

V

H

T

I

I

Y

I

T

M

N

N

-

G

-

A

-

N

-

H

-

R

-
x
M

-

D

-

G

-

S

R

T

H

Y

P

P

V

F

E

N

V

L

K

-

E

F

R

G

R

N

E

G

W

W

E

E

D

K

C

H

A

M

P

P

-

K

-

L

-

D

-

Q

-

L

-

P

-

I

-

K

-

G

-

D

V

T

T

I

I

I

G

G

E

N

E

D

C

V

W

W

I

I

G

G

G

K

H

D

S

V

T

V

I

I

C

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

R

G

A

A

V
x
I

I

V

G
x
A

A

A

G

N

S

S

V

V

V
|
V

K

K

D

D

I

I

P

A

A

P

D

Y

S

M

L
|
L

A

A

V
x
G

G

G

N

N

P
|
P

A

A

K

N

V

E

V
x
I

K

K

K

Q

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: M84 (vs. gap), I139 (≠ V154), A141 (≠ G156), V147 (= V162), L155 (= L170), G157 (≠ V172), P160 (= P175), I164 (≠ V179)

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
38% identity, 48% coverage: 94:181/184 of query aligns to 61:166/204 of 1mrlA

query
sites
1mrlA

V

L

T

K

I

I

G

G

K

K

K

F

T

C

L

S

I

I

A

G

P

P

G

G

V

V

H

T

I

I

Y

I

T

M

N

N

-

G

-

A

-
x
N

-
x
H

-

R

-

M

-

D

-

G

-

S

R

T

H

Y

P

P

V

F

E

N

V
x
L

K

-

E

F

R

G

R

N

E

G

W

W

E

E

D

K

C

H

A
x
M

P

P

-

K

-

L

-

D

-

Q

-
x
L

-

P

-

I

-

K

-

G

-

D

V

T

T

I

I

I

G

G

E

N

E

D

C

V

W

W

I

I

G

G

G

K

H

D

S

V

T

V

I

I

C

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

R

G

A

A

V

I

I

V

G

A

A

A

G

N

S

S

V

V

K

K

D

D

I

I

P

A

A

P

D

Y

S

M

L

L

A

A

V

G

G

G

N

N

P

P

A

A

K

N

V

E

V

I

K

K

K

Q

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: N81 (vs. gap), H82 (vs. gap), L93 (≠ V117), M102 (≠ A127), L108 (vs. gap)

Sites not aligning to the query:

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: 37, 39, 54, 56

1kk4A Crystal structure of vat(d) in complex with acetyl-coa (see paper)
38% identity, 48% coverage: 94:181/184 of query aligns to 61:166/205 of 1kk4A

query
sites
1kk4A

V

L

T

K

I

I

G

G

K

K

K

F

T

C

L

S

I
|
I

A
x
G

P

P

G

G

V

V

H

T

I

I

Y

I

T

M

N

N

-

G

-

A

-

N

-

H

-

R

-

M

-

D

-

G

-

S

R

T

H

Y

P

P

V

F

E

N

V

L

K

-

E

F

R

G

R

N

E

G

W

W

E

E

D

K

C

H

A

M

P

P

-

K

-

L

-

D

-

Q

-

L

-

P

-

I

-
x
K

-

G

-

D

V

T

T

I

I

I

G

G

E

N

E

D

C

V

W
|
W

I

I

G
|
G

G
x
K

H

D

S

V

T

V

I

I

C

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

R

G

A

A

V

I

I

V

G
x
A

A
|
A

G

N

S

S

V

V

V
|
V

K
|
K

D

D

I

I

P

A

A

P

D

Y

S

M

L
|
L

A

A

V
x
G

G
|
G

N

N

P
|
P

A

A

K

N

V

E

V
x
I

K

K

K

Q

binding acetyl coenzyme *a: I69 (= I102), G70 (≠ A103), K111 (vs. gap), W121 (= W136), G123 (= G138), K124 (≠ G139), A141 (≠ G156), A142 (= A157), V147 (= V162), K148 (= K163), L155 (= L170), G157 (≠ V172), G158 (= G173), P160 (= P175), I164 (≠ V179)

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
38% identity, 48% coverage: 94:181/184 of query aligns to 61:166/206 of 1khrA

query
sites
1khrA

V

L

T

K

I

I

G

G

K

K

K

F

T

C

L

S

I

I

A

G

P

P

G

G

V

V

H

T

I

I

Y

I

T

M

N

N

-

G

-
x
A

-
x
N

-
x
H

-

R

-
x
M

-

D

-

G

-

S

R

T

H

Y

P

P

V

F

E

N

V
x
L

K

-

E

F

R

G

R

N

E

G

W

W

E

E

D

K

C

H

A

M

P

P

-

K

-

L

-

D

-

Q

-

L

-

P

-

I

-

K

-

G

-

D

V

T

T

I

I

I

G

G

E

N

E

D

C

V

W
|
W

I

I

G

G

G

K

H

D

S

V

T

V

I

I

C

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

R

G

A

A

V

I

I

V

G
x
A

A
|
A

G

N

S

S

V

V

V
|
V

K

K

D

D

I

I

P

A

A

P

D

Y

S

M

L
|
L

A

A

V
x
G

G

G

N

N

P
|
P

A

A

K

N

V

E

V
x
I

K

K

K

Q

binding coenzyme a: H82 (vs. gap), M84 (vs. gap), W121 (= W136), A141 (≠ G156), A142 (= A157), V147 (= V162), L155 (= L170), G157 (≠ V172), P160 (= P175), I164 (≠ V179)
binding virginiamycin m1: A80 (vs. gap), N81 (vs. gap), H82 (vs. gap), L93 (≠ V117)

Sites not aligning to the query:

binding virginiamycin m1: 37, 39, 54

Query Sequence

>WP_015816780.1 NCBI__GCF_000023025.1:WP_015816780.1
MKSELEKMVAGEPYDAHCEEMLEIRTRVKRILHKLNVTEYYTENFKAVTHELCPNSAKDL
HLEPPFYCDYGENIHAAEGVFINFGAVILDGSTVTIGKKTLIAPGVHIYTNRHPVEVKER
REWEDCAPVTIGEECWIGGHSTICPGVTIGDRAVIGAGSVVVKDIPADSLAVGNPAKVVK
KLNQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory