SitesBLAST

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
41% identity, 98% coverage: 6:570/578 of query aligns to 99:660/670 of P17597

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P17597

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A122 (= A29) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ L31) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E51) binding thiamine diphosphate
S186 (= S93) binding FAD
P197 (≠ V104) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ D106) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q114) binding thiamine diphosphate
K220 (= K127) binding (R)-imazaquin
R246 (= R153) binding (R)-imazaquin; binding FAD
K256 (= K163) binding chlorimuron-ethyl
G308 (= G215) binding FAD
TL 331:332 (= TL 240:241) binding FAD
C340 (≠ G249) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (= LGMH 258:261) binding FAD
GVRFD 371:375 (≠ GARFD 280:284) binding FAD
DR 376:377 (= DR 285:286) binding chlorimuron-ethyl
DI 395:396 (= DI 304:305) binding FAD
DV 414:415 (≠ PV 323:324) binding FAD
QH 487:488 (= QH 401:402) binding thiamine diphosphate
GG 508:509 (= GG 422:423) binding FAD
GAM 511:513 (≠ GTM 425:427) binding thiamine diphosphate
D538 (≠ E452) binding Mg(2+)
DGS 538:540 (≠ EGS 452:454) binding thiamine diphosphate
N565 (= N479) binding Mg(2+)
NQHLGM 565:570 (≠ NQALGM 479:484) binding thiamine diphosphate
H567 (≠ A481) binding Mg(2+)
W574 (= W488) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ P563) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
41% identity, 98% coverage: 6:570/578 of query aligns to 14:575/582 of 7tzzA

query
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7tzzA

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V

binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M260), R292 (= R286), W489 (= W488), S568 (≠ P563)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), G452 (= G451), D453 (≠ E452), G454 (= G453), S455 (= S454), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), M263 (= M257), L264 (= L258), M266 (= M260), H267 (= H261), G286 (= G280), R288 (= R282), V293 (= V287), D310 (= D304), I311 (= I305), D329 (≠ P323), V330 (= V324), M405 (= M404), G423 (= G422)
binding magnesium ion: A37 (= A29), T82 (= T74), S83 (= S75), Q122 (= Q114), Y381 (≠ E380), D453 (≠ E452), M458 (= M457), Q461 (= Q460), N480 (= N479), H482 (≠ A481), K533 (≠ R526)

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
41% identity, 98% coverage: 6:570/578 of query aligns to 14:575/583 of 5k3sA

query
sites
5k3sA

G

G

G

A

D

D

I

I

L

L

I

V

R

E

S

A

L

L

A

E

D

R

E

Q

G

G

V

V

E

E

T

T

V

V

F

F

G

A

Y
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Y

P

P

G
|
G

A
|
A

A
x
S

L

M

H

E

I

I

Y

H

D

Q

A

A

I

L

F

T

R

R

Q

S

K

S

Q

S

V

I

K

R

H

N

V

V

L

L

V

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R

R

H

H

E
|
E

Q

Q

A

G

T

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

S

S

T

S

G

G

K

K

T

P

G

G

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V

C

L

I

V

A

T
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T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

I

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

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D

S

S

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V

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P

M

L

V

V

V

A

I

I

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G

G

Q

Q

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V

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S

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D

R

K

M

I

I

G

G

E

T

D

D

A

A

F
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F

Q
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Q

E
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E

T

T

D

P

M

I

V

V

G

E

V

V

S

T

R

R

P

S

I

I

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T

K

K

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Y

L

L

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K

M

D

D

Q

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K

E

D

D

I

I

A

P

E

R

T

I

V

I

K

E

A

A

F

F

Y

F

I

L

A

A

S

T

T

S

G

G

R
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R

P

P

G

G

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P

V

V

V

L

I

V

D

D

I

V

P

P

K
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K

D

D

I

I

T

Q

N

Q

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Q

V

L

D

A

K

I

V

P

E

N

Y

W

H

E

Y

-

P

-

E

Q

K

A

I

M

K

R

L

L

R

P

S

G

Y

Y

-

M

-

S

-

R

-

M

-

P

T

K

P

P

A

P

S

E

K

D

G

S

H

H

S

L

G

E

Q

Q

I

I

K

-

A

-

V

V

Q

R

L

L

M

I

L

S

T

E

A

S

R

K

P

P

M

V

I

L

Y

Y

A

V

G
|
G

V

C

V

L

Q

-

G

-

N

N

A

S

S

S

A

D

Q

E

L

L

T

G

A

R

I

F

A

V

Q

E

M

L

L

T

N

G

F

I

P

P

V

V

T

A

N

S

T
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T

L
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L

M
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M

G

G

L

L

G

G

A

S

Y

Y

P

P

G

C

T

D

D

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S

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L

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H

M

M

L
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L

G

G

M
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M

H

H

G

G

T

T

V

V

E

Y

A

A

N

N

T

Y

A

A

M

V

H

E

H

H

A

S

D

D

V

L

I

L

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L

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A

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F

G
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G

A

V

R
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R

F

F

D
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D

D

D

R
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R

V
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V

T

T

N

G

T

K

P

L

S

E

K

A

F

F

C

A

P

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S

R

A

A

K

K

I

I

I

V

H

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I

D
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D

I
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I

D

D

P

S

A

A

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E

I

I

S

G

K

K

T

N

V

K

A

T

A

P

D

H

I

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P

S

I

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V

C

G

G

P
x
D

V
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V

E

K

S

L

V

A

L

L

D

Q

E

G

M

M

L

N

S

K

L

V

L

L

N

E

E

-

T

-

N

N

E

R

R

A

P

E

D

E

A

L

K

K

A

L

I

D

A

F

G

G

W

V

W

W

K

R

-

N

E

E

I

L

D

N

E

V

W

Q

R

K

N

Q

R

K

Y

F

G

P

I

L

L

-

T

-

K

-

P

-

R

S

Y

F

D

K

T

T

S

F

G

G

D

E

M

A

L

I

M

P

P

P

Q

Q

E

Y

V

A

V

I

R

K

A

V

L

L

F

D

E

E

V

L

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T

N

D

G

G

D

K

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A

Y

I

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I

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S

S

T

D

G

V
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V

G
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G

Q
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Q

H
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H

Q

Q

M
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M

F

W

S

A

A

A

Q

Q

Y

F

Y

Y

Q

N

F

Y

D

K

K

K

P

P

R

R

R

Q

W

W

I

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N

S

S

S

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G

G

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L

L

G
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G

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A

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M

G

G

F

F

G

G

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L

P

P

A

A

M

I

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G

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A

Q

S

M

V

A

A

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N

R

P

D

D

A

A

V

I

V

V

A

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C

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V

I

T

D

G

G

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x
D

G
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G

S
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S

I

F

Q

I

M

M

C

N

I

V

Q

Q

E

E

L

L

S

A

T

T

C

I

T

R

Q

V

Y

E

H

N

L

L

P

P

V

V

K

K

I

V

I

L

C

L

L

L

N

N

N
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N

Q

Q

A
x
H

L
|
L

G
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G

M
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M

V
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V

K

M

Q

Q

W
|
W

Q

E

D

D

M

R

Q

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

E

H

S

T

L

F

Y

L

E

G

D

D

S

P

-

A

-

Q

-

E

-

D

-

E

-

I

L

F

P

P

D

N

F

M

V

L

K

L

L

F

M

A

D

A

A

A

Y

C

G

G

H

I

V

P

G

A

M

A

R

R

V

V

T

T

K

K

R

K

D

A

E

D

L

L

K

R

A

E

K

A

L

I

E

Q

E

T

C

M

F

L

A

D

M

T

K

P

D

G

R

-

C

P

V

Y

F

L

M

L

D

D

I

V

Y

I

V

C

D

P

P
x
H

K

Q

E

E

H

H

V

V

Y

L

P

P

M

M

Q

-

V

I

A

P

P

S

N
x
G

G

G

S

T

M

F

R

N

D

D

M

V

active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ A30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (= V287), V400 (= V399), G426 (= G425), M428 (= M427), D453 (≠ E452), N480 (= N479), H482 (≠ A481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ P552)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R286), M485 (= M484), W489 (= W488), G569 (≠ N564)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), L264 (= L258), M266 (= M260), G286 (= G280), R288 (= R282), D290 (= D284), V293 (= V287), D310 (= D304), I311 (= I305), D329 (≠ P323), V330 (= V324), M405 (= M404), G423 (= G422)
binding magnesium ion: D453 (≠ E452), N480 (= N479), H482 (≠ A481)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), D453 (≠ E452), G454 (= G453), S455 (= S454), N480 (= N479), H482 (≠ A481), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
41% identity, 98% coverage: 6:570/578 of query aligns to 14:575/582 of 8et4A

query
sites
8et4A

G

G

G

A

D

D

I

I

L

L

I

V

R

E

S

A

L

L

A

E

D

R

E

Q

G

G

V

V

E

E

T

T

V

V

F

F

G

A

Y

Y

P

P

G

G

A

A

A

S

L

M

H

E

I

I

Y

H

D

Q

A

A

I

L

F

T

R

R

Q

S

K

S

Q

S

V

I

K

R

H

N

V

V

L

L

V

P

R

R

H

H

E

E

Q

Q

A

G

T

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

S

S

T

S

G

G

K

K

T

P

G

G

V

I

V

C

L

I

V

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

I

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

I

V

P

P

M

L

V

V

V

A

I

I

S

T

G

G

Q

Q

V

V

V

P

S

R

D

R

K

M

I

I

G

G

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T

D

D

A

A

F

F

Q

Q

E

E

T

T

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P

M

I

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V

G

E

V

V

S

T

R

R

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S

I

I

V

T

K

K

H

H

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N

F

Y

L

L

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D

D

Q

V

K

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D

D

I

I

A

P

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R

T

I

V

I

K

E

A

A

F

F

Y

F

I

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A

A

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T

S

G

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R

P

P

G

G

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P

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V

L

I

V

D

D

I

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K

D

D

I

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N

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W

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A

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M

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L

Y

Y

A

V

G
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G

V

C

V

L

Q

-

G

-

N

N

A

S

S

S

A

D

Q

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L

L

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A

R

I

F

A

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Q

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M

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T

L
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L

M
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M

G

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L

G

G

A

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Y

Y

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D

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M

L
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L

G

G

M
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M

H
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H

G

G

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T

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V

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Y

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A

N

N

T

Y

A

A

M

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H

H

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G

A
x
V

R
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R

F

F

D
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D

D

D

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R

V
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V

T

T

N

G

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K

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D

I
|
I

D

D

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A

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K

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K

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D

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V

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M

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L

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H
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W

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M

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K

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N
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N

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x
H

L
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L

G
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G

M
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M

V
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K

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K

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H

V

V

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L

P

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M

M

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S

N

G

G

G

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N

D

D

M

V

binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), G452 (= G451), D453 (≠ E452), G454 (= G453), S455 (= S454), M458 (= M457), N480 (= N479), H482 (≠ A481), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), L264 (= L258), M266 (= M260), H267 (= H261), G286 (= G280), V287 (≠ A281), R288 (= R282), D290 (= D284), R292 (= R286), V293 (= V287), D310 (= D304), I311 (= I305), D329 (≠ P323), V330 (= V324), M405 (= M404), G423 (= G422)
binding magnesium ion: F370 (≠ Y369), D453 (≠ E452), M458 (= M457), Q461 (= Q460), N480 (= N479), H482 (≠ A481), K533 (≠ R526)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M260), R292 (= R286), M485 (= M484), W489 (= W488), S568 (≠ P563)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
41% identity, 98% coverage: 6:570/578 of query aligns to 14:575/582 of 5wj1A

query
sites
5wj1A

G

G

G

A

D

D

I

I

L

L

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L

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R

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K

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I

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G

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N

N

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L

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I

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x
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D

E

A

L

K

K

A

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A

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W

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N

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Q

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C

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x
H

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L

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M

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V

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E

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F

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Y

D

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G

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-

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-

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-

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-

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-

I

L

F

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P

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N

F

M

V

L

K

L

L

F

M

A

D

A

A

A

Y

C

G

G

H

I

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P

G

A

M

A

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R

V

V

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T

K

K

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K

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A

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R

A

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K

A

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E

Q

E

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M

F

L

A

D

M

T

K

P

D

G

R

-

C

P

V

Y

F

L

M

L

D

D

I

V

Y

I

V

C

D

P

P
x
H

K

Q

E

E

H

H

V

V

Y

L

P

P

M

M

Q

-

V

I

A

P

P
x
S

N

G

G

G

S

T

M

F

R

N

D

D

M

V

active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ A30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (= V287), V400 (= V399), G426 (= G425), M428 (= M427), D453 (≠ E452), N480 (= N479), H482 (≠ A481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ P552)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), M263 (= M257), L264 (= L258), G286 (= G280), R288 (= R282), V293 (= V287), D310 (= D304), I311 (= I305), D329 (≠ P323), V330 (= V324), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (≠ E452), N480 (= N479), H482 (≠ A481)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M260), D291 (= D285), R292 (= R286), M485 (= M484), W489 (= W488), S568 (≠ P563)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), M428 (= M427), D453 (≠ E452), G454 (= G453), S455 (= S454), M458 (= M457), N480 (= N479), H482 (≠ A481), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
41% identity, 98% coverage: 6:570/578 of query aligns to 14:575/582 of 5k6tA

query
sites
5k6tA

G

G

G

A

D

D

I

I

L

L

I

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A

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L

A

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D

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G

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V

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E

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T

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F

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Y

P

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A
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A
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A

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E

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A

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A

A

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E

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Y

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A

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S

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G

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P

G

G

A

A

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T

N

N

A

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P

P
x
H

K

Q

E

E

H

H

V

V

Y

L

P

P

M

M

Q

-

V

I

A

P

P
x
S

N

G

G

G

S

T

M

F

R

N

D

D

M

V

active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ A30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (= V287), V400 (= V399), G426 (= G425), M428 (= M427), D453 (≠ E452), N480 (= N479), H482 (≠ A481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ P552)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H261), R292 (= R286), M485 (= M484), W489 (= W488), S568 (≠ P563)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), L264 (= L258), G286 (= G280), R288 (= R282), D290 (= D284), R292 (= R286), V293 (= V287), D310 (= D304), I311 (= I305), D329 (≠ P323), V330 (= V324), Q404 (= Q403), M405 (= M404), G423 (= G422)
binding magnesium ion: D453 (≠ E452), N480 (= N479), H482 (≠ A481)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), G452 (= G451), G454 (= G453), S455 (= S454), N480 (= N479), H482 (≠ A481), L483 (= L482), G484 (= G483)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
41% identity, 98% coverage: 6:570/578 of query aligns to 14:575/582 of 5k6rA

query
sites
5k6rA

G

G

G

A

D

D

I

I

L

L

I

V

R

E

S

A

L

L

A

E

D

R

E

Q

G

G

V

V

E

E

T

T

V

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

A
x
S

L

M

H

E

I

I

Y

H

D

Q

A

A

I

L

F

T

R

R

Q

S

K

S

Q

S

V

I

K

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

A

G

T

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

S

S

T

S

G

G

K

K

T

P

G

G

V

I

V

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

I

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

I

V

P

P

M

L

V

V

V

A

I

I

S

T

G

G

Q

Q

V

V

V

P

S

R

D

R

K

M

I

I

G

G

E

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

T

D

P

M

I

V

V

G

E

V

V

S

T

R

R

P

S

I

I

V

T

K

K

H

H

S

N

F

Y

L

L

V

V

K

M

D

D

Q

V

K

E

D

D

I

I

A

P

E

R

T

I

V

I

K

E

A

A

F

F

Y

F

I

L

A

A

S

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

N

Q

P

Q

V

L

D

A

K

I

V

P

E

N

Y

W

H

E

Y

-

P

-

E

Q

K

A

I

M

K

R

L

L

R

P

S

G

Y

Y

-

M

-

S

-

R

-

M

-

P

T

K

P

P

A

P

S

E

K

D

G

S

H

H

S

L

G

E

Q

Q

I

I

K

-

A

-

V

V

Q

R

L

L

M

I

L

S

T

E

A

S

R

K

P

P

M

V

I

L

Y

Y

A

V

G
|
G

V

C

V

L

Q

-

G

-

N

N

A

S

S

S

A

D

Q

E

L

L

T

G

A

R

I

F

A

V

Q

E

M

L

L

T

N

G

F

I

P

P

V

V

T

A

N

S

T
|
T

L
|
L

M
|
M

G

G

L

L

G

G

A

S

Y

Y

P

P

G

C

T

D

D

D

R

E

Q

L

F

S

L

L

G

H

M

M

L
|
L

G

G

M
|
M

H

H

G

G

T

T

V

V

E

Y

A

A

N

N

T

Y

A

A

M

V

H

E

H

H

A

S

D

D

V

L

I

L

F

L

A

A

V

F

G
|
G

A

V

R
|
R

F

F

D

D

R
|
R

V
|
V

T

T

N

G

T

K

P

L

S

E

K

A

F

F

C

A

P

S

S

R

A

A

K

K

I

I

I

V

H

H

V

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

T

N

V

K

A

T

A

P

D

H

I

V

P

S

I

V

V

C

G
|
G

P
x
D

V
|
V

E

K

S

L

V

A

L

L

D

Q

E

G

M

M

L

N

S

K

L

V

L

L

N

E

E

-

T

-

N

N

E

R

R

A

P

E

D

E

A

L

K

K

A

L

I

D

A

F

G

G

W

V

W

W

K

R

-

N

E

E

I

L

D

N

E

V

W

Q

R

K

N

Q

R

K

Y

F

G

P

I

L

L

-

T

-

K

-

P

-

R

S

Y

F

D

K

T

T

S

F

G

G

D

E

M

A

L

I

M

P

P

P

Q

Q

E

Y

V

A

V

I

R

K

A

V

L

L

F

D

E

E

V

L

T

T

N

D

G

G

D

K

A

A

Y

I

V

I

C

S

S

T

D

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

F

W

S

A

A

A

Q

Q

Y

F

Y

Y

Q

N

F

Y

D

K

K

K

P

P

R

R

R

Q

W

W

I

L

N

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

V

A

Q

S

M

V

A

A

H

N

R

P

D

D

A

A

V

I

V

V

A

V

C

D

V

I

T

D

G

G

E
x
D

G
|
G

S
|
S

I

F

Q

I

M
|
M

C

N

I

V

Q

Q

E

E

L

L

S

A

T

T

C

I

T

R

Q

V

Y

E

H

N

L

L

P

P

V

V

K

K

I

V

I

L

C

L

L

L

N

N

N
|
N

Q

Q

A
x
H

L
|
L

G
|
G

M
|
M

V
|
V

K

M

Q

Q

W
|
W

Q

E

D

D

M

R

Q

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

E

H

S

T

L

F

Y

L

E

G

D

D

S

P

-

A

-

Q

-

E

-

D

-

E

-

I

L

F

P

P

D

N

F

M

V

L

K

L

L

F

M

A

D

A

A

A

Y

C

G

G

H

I

V

P

G

A

M

A

R

R

V

V

T

T

K

K

R

K

D

A

E

D

L

L

K

R

A

E

K

A

L

I

E

Q

E

T

C

M

F

L

A

D

M

T

K

P

D

G

R

-

C

P

V

Y

F

L

M

L

D

D

I

V

Y

I

V

C

D

P

P
x
H

K

Q

E

E

H

H

V

V

Y

L

P

P

M

M

Q

-

V

I

A

P

P
x
S

N

G

G

G

S

T

M

F

R

N

D

D

M

V

active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ A30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (= V287), V400 (= V399), G426 (= G425), M428 (= M427), D453 (≠ E452), N480 (= N479), H482 (≠ A481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ P552)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R286), W489 (= W488), S568 (≠ P563)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), L264 (= L258), M266 (= M260), G286 (= G280), R288 (= R282), R292 (= R286), V293 (= V287), D310 (= D304), I311 (= I305), G328 (= G322), D329 (≠ P323), V330 (= V324), M405 (= M404), G423 (= G422)
binding magnesium ion: D453 (≠ E452), N480 (= N479), H482 (≠ A481)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), D453 (≠ E452), G454 (= G453), S455 (= S454), M458 (= M457), N480 (= N479), H482 (≠ A481), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
41% identity, 98% coverage: 6:570/578 of query aligns to 14:575/582 of 1z8nA

query
sites
1z8nA

G

G

G

A

D

D

I

I

L

L

I

V

R

E

S

A

L

L

A

E

D

R

E

Q

G

G

V

V

E

E

T

T

V

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

A
x
S

L

M

H

E

I

I

Y

H

D

Q

A

A

I

L

F

T

R

R

Q

S

K

S

Q

S

V

I

K

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

A

G

T

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

S

S

T

S

G

G

K

K

T

P

G

G

V

I

V

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

I

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

I

V

P

P

M

L

V

V

V

A

I

I

S

T

G

G

Q

Q

V

V

V

P

S

R

D

R

K

M

I

I

G

G

E

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

T

D

P

M

I

V

V

G

E

V

V

S

T

R

R

P

S

I

I

V

T

K
|
K

H

H

S

N

F

Y

L

L

V

V

K

M

D

D

Q

V

K

E

D

D

I

I

A

P

E

R

T

I

V

I

K

E

A

A

F

F

Y

F

I

L

A

A

S

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

N

Q

P

Q

V

L

D

A

K

I

V

P

E

N

Y

W

H

E

Y

-

P

-

E

Q

K

A

I

M

K

R

L

L

R

P

S

G

Y
|
Y

-

M

-

S

-
x
R

-

M

-

P

T

K

P

P

A

P

S

E

K

D

G

S

H

H

S

L

G

E

Q

Q

I

I

K

-

A

-

V

V

Q

R

L

L

M

I

L

S

T

E

A

S

R

K

P

P

M

V

I

L

Y

Y

A

V

G
|
G

G

G

G
|
G

V

C

V

L

Q

-

G

-

N

N

A

S

S

S

A

D

Q

E

L

L

T

G

A

R

I

F

A

V

Q

E

M

L

L

T

N

G

F

I

P

P

V

V

T

A

N

S

T
|
T

L
|
L

M
|
M

G

G

L

L

G

G

A

S

Y

Y

P

P

G

C

T

D

D

D

R

E

Q

L

F

S

L

L

G

H

M

M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

T

T

V

V

E

Y

A

A

N

N

T

Y

A

A

M

V

H

E

H

H

A

S

D

D

V

L

I

L

F

L

A

A

V

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

N

G

T

K

P

L

S

E

K

A

F

F

C

A

P

S

S

R

A

A

K

K

I

I

I

V

H

H

V

I

D
|
D

I
|
I

D
|
D

P

S

A

A

S

E

I

I

S

G

K

K

T

N

V

K

A

T

A

P

D

H

I

V

P

S

I

V

V

C

G

G

P
x
D

V
|
V

E

K

S

L

V

A

L

L

D

Q

E

G

M

M

L

N

S

K

L

V

L

L

N

E

E

-

T

-

N

N

E

R

R

A

P

E

D

E

A

L

K

K

A

L

I

D

A

F

G

G

W

V

W

W

K

R

-

N

E

E

I

L

D

N

E

V

W

Q

R

K

N

Q

R

K

Y

F

G

P

I

L

L

-

T

-

K

-

P

-

R

S

Y

F

D

K

T

T

S

F

G

G

D

E

M

A

L

I

M

P

P

P

Q

Q

E

Y

V

A

V

I

R

K

A

V

L

L

F

D

E

E

V

L

T

T

N

D

G

G

D

K

A

A

Y

I

V

I

C

S

S

T

D

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

F

W

S

A

A

A

Q

Q

Y

F

Y

Y

Q

N

F

Y

D

K

K

K

P

P

R

R

R

Q

W

W

I

L

N

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

V

A

Q

S

M

V

A

A

H

N

R

P

D

D

A

A

V

I

V

V

A

V

C

D

V

I

T

D

G
|
G

E
x
D

G
|
G

S
|
S

I

F

Q

I

M

M

C

N

I

V

Q

Q

E

E

L

L

S

A

T

T

C

I

T

R

Q

V

Y

E

H

N

L

L

P

P

V

V

K

K

I

V

I

L

C

L

L

L

N

N

N
|
N

Q

Q

A
x
H

L
|
L

G
|
G

M
|
M

V
|
V

K

M

Q

Q

W
|
W

Q

E

D

D

M

R

Q

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

E

H

S

T

L

F

Y

L

E

G

D

D

S

P

-

A

-

Q

-

E

-

D

-

E

-

I

L

F

P

P

D

N

F

M

V

L

K

L

L

F

M

A

D

A

A

A

Y

C

G

G

H

I

V

P

G

A

M

A

R

R

V

V

T

T

K

K

R

K

D

A

E

D

L

L

K

R

A

E

K

A

L

I

E

Q

E

T

C

M

F

L

A

D

M

T

K

P

D

G

R

-

C

P

V

Y

F

L

M

L

D

D

I

V

Y

I

V

C

D

P

P
x
H

K

Q

E

E

H

H

V

V

Y

L

P

P

M

M

Q

-

V

I

A

P

P

S

N
x
G

G

G

S

T

M

F

R

N

D

D

M

V

active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ A30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (= V287), V400 (= V399), G426 (= G425), M428 (= M427), D453 (≠ E452), N480 (= N479), H482 (≠ A481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ P552)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K127), R161 (= R153), Y191 (= Y185), R194 (vs. gap), D291 (= D285), R292 (= R286), D312 (= D306), W489 (= W488), G569 (≠ N564)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), L264 (= L258), G265 (= G259), M266 (= M260), H267 (= H261), G286 (= G280), V287 (≠ A281), R288 (= R282), D290 (= D284), R292 (= R286), V293 (= V287), D310 (= D304), I311 (= I305), D329 (≠ P323), V330 (= V324), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (≠ E452), N480 (= N479)
binding thiamine diphosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), G452 (= G451), G454 (= G453), S455 (= S454), N480 (= N479), H482 (≠ A481), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
41% identity, 98% coverage: 6:570/578 of query aligns to 14:575/582 of 1yi1A

query
sites
1yi1A

G

G

G

A

D

D

I

I

L

L

I

V

R

E

S

A

L

L

A

E

D

R

E

Q

G

G

V

V

E

E

T

T

V

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

A
x
S

L

M

H

E

I

I

Y

H

D

Q

A

A

I

L

F

T

R

R

Q

S

K

S

Q

S

V

I

K

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

A

G

T

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

S

S

T

S

G

G

K

K

T

P

G

G

V

I

V

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

I

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

I

V

P

P

M

L

V

V

V

A

I

I

S

T

G

G

Q

Q

V

V

V

P

S

R

D

R

K

M

I

I

G

G

E

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

T

D

P

M

I

V

V

G

E

V

V

S

T

R

R

P

S

I

I

V

T

K

K

H

H

S

N

F

Y

L

L

V

V

K

M

D

D

Q

V

K

E

D

D

I

I

A

P

E

R

T

I

V

I

K

E

A

A

F

F

Y

F

I

L

A

A

S

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

N

Q

P

Q

V

L

D

A

K

I

V

P

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N

Y

W

H

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Y

-

P

-

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Q

K

A

I

M

K

R

L

L

R

P

S

G

Y

Y

-

M

-

S

-

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-

M

-

P

T

K

P

P

A

P

S

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K

D

G

S

H

H

S

L

G

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Q

Q

I

I

K

-

A

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V

V

Q

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L

L

M

I

L

S

T

E

A

S

R

K

P

P

M

V

I

L

Y

Y

A

V

G

G

G
|
G

V

C

V

L

Q

-

G

-

N

N

A

S

S

S

A

D

Q

E

L

L

T

G

A

R

I

F

A

V

Q

E

M

L

L

T

N

G

F

I

P

P

V

V

T

A

N

S

T
|
T

L
|
L

M
|
M

G

G

L

L

G

G

A

S

Y

Y

P

P

G

C

T

D

D

D

R

E

Q

L

F

S

L

L

G

H

M
|
M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

T

T

V

V

E

Y

A

A

N

N

T

Y

A

A

M

V

H

E

H

H

A

S

D

D

V

L

I

L

F

L

A

A

V

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

N

G

T

K

P

L

S

E

K

A

F

F

C

A

P

S

S

R

A

A

K

K

I

I

I

V

H

H

V

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

T

N

V

K

A

T

A

P

D

H

I

V

P

S

I

V

V

C

G

G

P
x
D

V
|
V

E

K

S

L

V

A

L

L

D

Q

E

G

M

M

L

N

S

K

L

V

L

L

N

E

E

-

T

-

N

N

E

R

R

A

P

E

D

E

A

L

K

K

A

L

I

D

A

F

G

G

W

V

W

W

K

R

-

N

E

E

I

L

D

N

E

V

W

Q

R

K

N

Q

R

K

Y

F

G

P

I

L

L

-

T

-

K

-

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-

R

S

Y

F

D

K

T

T

S

F

G

G

D

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M

A

L

I

M

P

P

P

Q

Q

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Y

V

A

V

I

R

K

A

V

L

L

F

D

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E

V

L

T

T

N

D

G

G

D

K

A

A

Y

I

V

I

C

S

S

T

D

G

V
|
V

G

G

Q

Q

H

H

Q

Q

M
|
M

F

W

S

A

A

A

Q

Q

Y

F

Y

Y

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N

F

Y

D

K

K

K

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P

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R

R

Q

W

W

I

L

N

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

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A

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M

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A

A

H

N

R

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D

D

A

A

V

I

V

V

A

V

C

D

V

I

T

D

G

G

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x
D

G

G

S

S

I

F

Q

I

M

M

C

N

I

V

Q

Q

E

E

L

L

S

A

T

T

C

I

T

R

Q

V

Y

E

H

N

L

L

P

P

V

V

K

K

I

V

I

L

C

L

L

L

N

N

N
|
N

Q

Q

A
x
H

L
|
L

G

G

M
|
M

V
|
V

K

M

Q

Q

W
|
W

Q

E

D

D

M

R

Q

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

E

H

S

T

L

F

Y

L

E

G

D

D

S

P

-

A

-

Q

-

E

-

D

-

E

-

I

L

F

P

P

D

N

F

M

V

L

K

L

L

F

M

A

D

A

A

A

Y

C

G

G

H

I

V

P

G

A

M

A

R

R

V

V

T

T

K

K

R

K

D

A

E

D

L

L

K

R

A

E

K

A

L

I

E

Q

E

T

C

M

F

L

A

D

M

T

K

P

D

G

R

-

C

P

V

Y

F

L

M

L

D

D

I

V

Y

I

V

C

D

P

P
x
H

K

Q

E

E

H

H

V

V

Y

L

P

P

M

M

Q

-

V

I

A

P

P
x
S

N

G

G

G

S

T

M

F

R

N

D

D

M

V

active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ A30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (= V287), V400 (= V399), G426 (= G425), M428 (= M427), D453 (≠ E452), N480 (= N479), H482 (≠ A481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ P552)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (= D285), R292 (= R286), W489 (= W488), S568 (≠ P563)
binding flavin-adenine dinucleotide: R161 (= R153), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), M263 (= M257), L264 (= L258), G265 (= G259), M266 (= M260), H267 (= H261), G286 (= G280), V287 (≠ A281), R288 (= R282), D290 (= D284), V293 (= V287), D310 (= D304), I311 (= I305), D329 (≠ P323), V330 (= V324), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (≠ E452), N480 (= N479), H482 (≠ A481)

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
41% identity, 98% coverage: 6:570/578 of query aligns to 14:575/582 of 1yi0A

query
sites
1yi0A

G

G

G

A

D

D

I

I

L

L

I

V

R

E

S

A

L

L

A

E

D

R

E

Q

G

G

V

V

E

E

T

T

V

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

A
x
S

L

M

H

E

I

I

Y

H

D

Q

A

A

I

L

F

T

R

R

Q

S

K

S

Q

S

V

I

K

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

A

G

T

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

S

S

T

S

G

G

K

K

T

P

G

G

V

I

V

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

I

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

I

V

P

P

M

L

V

V

V

A

I

I

S

T

G

G

Q

Q

V

V

V

P

S

R

D

R

K

M

I

I

G

G

E

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

T

D

P

M

I

V

V

G

E

V

V

S

T

R

R

P

S

I

I

V

T

K

K

H

H

S

N

F

Y

L

L

V

V

K

M

D

D

Q

V

K

E

D

D

I

I

A

P

E

R

T

I

V

I

K

E

A

A

F

F

Y

F

I

L

A

A

S

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

N

Q

P

Q

V

L

D

A

K

I

V

P

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N

Y

W

H

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Y

-

P

-

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K

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M

K

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L

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Y

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M

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M

-

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A

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M

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M

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I

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Y

Y

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G
|
G

V

C

V

L

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G

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N

N

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S

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L

L

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|
T

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|
L

M
|
M

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G

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L

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Y

Y

P

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G

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D

D

D

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L

L

G

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M

M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

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V

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Y

A

A

N

N

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A

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H

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|
G

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V

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|
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|
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|
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N

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|
D

I
|
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D

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K

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|
G

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D

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|
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A

L

L

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M

L

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N

N

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W

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|
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|
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|
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L

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|
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|
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L

L

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P

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V

K

K

I

V

I

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C

L

L

L

N

N

N
|
N

Q

Q

A
x
H

L
|
L

G

G

M
|
M

V
|
V

K

M

Q

Q

W
|
W

Q

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D

D

M

R

Q

F

Y

Y

D

K

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A

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N

Y

R

S

A

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F

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D

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A

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L

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L

L

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A

A

A

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G

G

H

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P

G

A

M

A

R

R

V

V

T

T

K

K

R

K

D

A

E

D

L

L

K

R

A

E

K

A

L

I

E

Q

E

T

C

M

F

L

A

D

M

T

K

P

D

G

R

-

C

P

V

Y

F

L

M

L

D

D

I

V

Y

I

V

C

D

P

P
x
H

K

Q

E

E

H

H

V

V

Y

L

P

P

M

M

Q

-

V

I

A

P

P
x
S

N

G

G

G

S

T

M

F

R

N

D

D

M

V

active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ A30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (= V287), V400 (= V399), G426 (= G425), M428 (= M427), D453 (≠ E452), N480 (= N479), H482 (≠ A481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ P552)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D285), R292 (= R286), W489 (= W488), S568 (≠ P563)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), L264 (= L258), G265 (= G259), M266 (= M260), H267 (= H261), G286 (= G280), V287 (≠ A281), R288 (= R282), D290 (= D284), R292 (= R286), V293 (= V287), D310 (= D304), I311 (= I305), G328 (= G322), D329 (≠ P323), V330 (= V324), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (≠ E452), N480 (= N479), H482 (≠ A481)

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
41% identity, 98% coverage: 6:570/578 of query aligns to 14:575/582 of 1yhzA

query
sites
1yhzA

G

G

G

A

D

D

I

I

L

L

I

V

R

E

S

A

L

L

A

E

D

R

E

Q

G

G

V

V

E

E

T

T

V

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

A
x
S

L

M

H

E

I

I

Y

H

D

Q

A

A

I

L

F

T

R

R

Q

S

K

S

Q

S

V

I

K

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

A

G

T

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

S

S

T

S

G

G

K

K

T

P

G

G

V

I

V

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

I

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

I

V

P

P

M

L

V

V

V

A

I

I

S

T

G

G

Q

Q

V

V

V

P

S

R

D

R

K

M

I

I

G

G

E

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

T

D

P

M

I

V

V

G

E

V

V

S

T

R

R

P

S

I

I

V

T

K

K

H

H

S

N

F

Y

L

L

V

V

K

M

D

D

Q

V

K

E

D

D

I

I

A

P

E

R

T

I

V

I

K

E

A

A

F

F

Y

F

I

L

A

A

S

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

N

Q

P

Q

V

L

D

A

K

I

V

P

E

N

Y

W

H

E

Y

-

P

-

E

Q

K

A

I

M

K

R

L

L

R

P

S

G

Y

Y

-

M

-

S

-

R

-

M

-

P

T

K

P

P

A

P

S

E

K

D

G

S

H

H

S

L

G

E

Q

Q

I

I

K

-

A

-

V

V

Q

R

L

L

M

I

L

S

T

E

A

S

R

K

P

P

M

V

I

L

Y

Y

A

V

G

G

G
|
G

V

C

V

L

Q

-

G

-

N

N

A

S

S

S

A

D

Q

E

L

L

T

G

A

R

I

F

A

V

Q

E

M

L

L

T

N

G

F

I

P

P

V

V

T

A

N

S

T
|
T

L
|
L

M
|
M

G

G

L

L

G

G

A

S

Y

Y

P

P

G

C

T

D

D

D

R

E

Q

L

F

S

L

L

G

H

M

M

L
|
L

G

G

M
|
M

H
|
H

G

G

T

T

V

V

E

Y

A

A

N

N

T

Y

A

A

M

V

H

E

H

H

A

S

D

D

V

L

I

L

F

L

A

A

V

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

N

G

T

K

P

L

S

E

K

A

F

F

C

A

P

S

S

R

A

A

K

K

I

I

I

V

H

H

V

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

T

N

V

K

A

T

A

P

D

H

I

V

P

S

I

V

V

C

G

G

P
x
D

V
|
V

E

K

S

L

V

A

L

L

D

Q

E

G

M

M

L

N

S

K

L

V

L

L

N

E

E

-

T

-

N

N

E

R

R

A

P

E

D

E

A

L

K

K

A

L

I

D

A

F

G

G

W

V

W

W

K

R

-

N

E

E

I

L

D

N

E

V

W

Q

R

K

N

Q

R

K

Y

F

G

P

I

L

L

-

T

-

K

-

P

-

R

S

Y

F

D

K

T

T

S

F

G

G

D

E

M

A

L

I

M

P

P

P

Q

Q

E

Y

V

A

V

I

R

K

A

V

L

L

F

D

E

E

V

L

T

T

N

D

G

G

D

K

A

A

Y

I

V

I

C

S

S

T

D

G

V
|
V

G

G

Q

Q

H

H

Q
|
Q

M
|
M

F

W

S

A

A

A

Q

Q

Y

F

Y

Y

Q

N

F

Y

D

K

K

K

P

P

R

R

R

Q

W

W

I

L

N

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

V

A

Q

S

M

V

A

A

H

N

R

P

D

D

A

A

V

I

V

V

A

V

C

D

V

I

T

D

G

G

E
x
D

G

G

S

S

I

F

Q

I

M

M

C

N

I

V

Q

Q

E

E

L

L

S

A

T

T

C

I

T

R

Q

V

Y

E

H

N

L

L

P

P

V

V

K

K

I

V

I

L

C

L

L

L

N

N

N
|
N

Q

Q

A
x
H

L
|
L

G

G

M
|
M

V
|
V

K

M

Q

Q

W
|
W

Q

E

D

D

M

R

Q

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

E

H

S

T

L

F

Y

L

E

G

D

D

S

P

-

A

-

Q

-

E

-

D

-

E

-

I

L

F

P

P

D

N

F

M

V

L

K

L

L

F

M

A

D

A

A

A

Y

C

G

G

H

I

V

P

G

A

M

A

R

R

V

V

T

T

K

K

R

K

D

A

E

D

L

L

K

R

A

E

K

A

L

I

E

Q

E

T

C

M

F

L

A

D

M

T

K

P

D

G

R

-

C

P

V

Y

F

L

M

L

D

D

I

V

Y

I

V

C

D

P

P
x
H

K

Q

E

E

H

H

V

V

Y

L

P

P

M

M

Q

-

V

I

A

P

P
x
S

N

G

G

G

S

T

M

F

R

N

D

D

M

V

active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ A30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (= V287), V400 (= V399), G426 (= G425), M428 (= M427), D453 (≠ E452), N480 (= N479), H482 (≠ A481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ P552)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (= D285), R292 (= R286), M485 (= M484), W489 (= W488), S568 (≠ P563)
binding flavin-adenine dinucleotide: R161 (= R153), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), L264 (= L258), M266 (= M260), H267 (= H261), G286 (= G280), V287 (≠ A281), R288 (= R282), D290 (= D284), V293 (= V287), D310 (= D304), I311 (= I305), D329 (≠ P323), V330 (= V324), Q404 (= Q403), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (≠ E452), N480 (= N479), H482 (≠ A481)

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
41% identity, 98% coverage: 6:570/578 of query aligns to 14:575/582 of 1yhyA

query
sites
1yhyA

G

G

G

A

D

D

I

I

L

L

I

V

R

E

S

A

L

L

A

E

D

R

E

Q

G

G

V

V

E

E

T

T

V

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

A
x
S

L

M

H

E

I

I

Y

H

D

Q

A

A

I

L

F

T

R

R

Q

S

K

S

Q

S

V

I

K

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

A

G

T

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

S

S

T

S

G

G

K

K

T

P

G

G

V

I

V

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

I

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

I

V

P

P

M

L

V

V

V

A

I

I

S

T

G

G

Q

Q

V

V

V

P

S

R

D

R

K

M

I

I

G

G

E

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

T

D

P

M

I

V

V

G

E

V

V

S

T

R

R

P

S

I

I

V

T

K

K

H

H

S

N

F

Y

L

L

V

V

K

M

D

D

Q

V

K

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D

D

I

I

A

P

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R

T

I

V

I

K

E

A

A

F

F

Y

F

I

L

A

A

S

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

I

V

P

P

K
|
K

D

D

I

I

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Q

N

Q

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Q

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L

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A

K

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Y

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H

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Y

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P

-

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K

A

I

M

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L

L

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Y

Y

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M

-

S

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-

M

-

P

T

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P

P

A

P

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H

H

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K

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A

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V

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L

L

M

I

L

S

T

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A

S

R

K

P

P

M

V

I

L

Y

Y

A

V

G
|
G

V

C

V

L

Q

-

G

-

N

N

A

S

S

S

A

D

Q

E

L

L

T

G

A

R

I

F

A

V

Q

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M

L

L

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N

G

F

I

P

P

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V

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A

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S

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|
T

L
|
L

M
|
M

G

G

L

L

G

G

A

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Y

Y

P

P

G

C

T

D

D

D

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L

L

G

H

M

M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

T

T

V

V

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Y

A

A

N

N

T

Y

A

A

M

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H

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H

H

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D

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L

I

L

F

L

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A

V

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G
|
G

A
x
V

R
|
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F

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|
D

R
|
R

V
|
V

T

T

N

G

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K

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K

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|
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I
|
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|
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|
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N

N
|
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H

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M

V
|
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|
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C

P

V

Y

F

L

M

L

D

D

I

V

Y

I

V

C

D

P

P
x
H

K

Q

E

E

H

H

V

V

Y

L

P

P

M

M

Q

-

V

I

A

P

P
x
S

N

G

G

G

S

T

M

F

R

N

D

D

M

V

active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ A30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (= V287), V400 (= V399), G426 (= G425), M428 (= M427), D453 (≠ E452), N480 (= N479), H482 (≠ A481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ P552)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D285), R292 (= R286), V486 (= V485), W489 (= W488), S568 (≠ P563)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), L264 (= L258), G265 (= G259), M266 (= M260), H267 (= H261), G286 (= G280), V287 (≠ A281), R288 (= R282), D290 (= D284), V293 (= V287), D310 (= D304), I311 (= I305), D329 (≠ P323), V330 (= V324), Q404 (= Q403), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (≠ E452), N480 (= N479), H482 (≠ A481)

Query Sequence

>WP_015818963.1 NCBI__GCF_000023025.1:WP_015818963.1
MEKLSGGDILIRSLADEGVETVFGYPGGAALHIYDAIFRQKQVKHVLVRHEQAATHAADG
YARSTGKTGVVLVTSGPGATNAITGIATAYMDSIPMVVISGQVVSDKIGEDAFQETDMVG
VSRPIVKHSFLVKDQKDIAETVKKAFYIASTGRPGPVVIDIPKDITNPVDKVEYHYPEKI
KLRSYTPASKGHSGQIKKAVQLMLTARRPMIYAGGGVVQGNASAQLTAIAQMLNFPVTNT
LMGLGAYPGTDRQFLGMLGMHGTVEANTAMHHADVIFAVGARFDDRVTNTPSKFCPSAKI
IHVDIDPASISKTVAADIPIVGPVESVLDEMLSLLNETNERPDAKAIAGWWKEIDEWRNR
YGILTKPRYDTSGDMLMPQEVVRALFEVTNGDAYVCSDVGQHQMFSAQYYQFDKPRRWIN
SGGLGTMGFGLPAAMGVQMAHRDAVVACVTGEGSIQMCIQELSTCTQYHLPVKIICLNNQ
ALGMVKQWQDMQYDSRYSESLYEDSLPDFVKLMDAYGHVGMRVTKRDELKAKLEECFAMK
DRCVFMDIYVDPKEHVYPMQVAPNGSMRDMWLSKTERT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory