SitesBLAST
Comparing WP_015820561.1 NCBI__GCF_000023025.1:WP_015820561.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8bcjB Crystal structure of short-chain dehydrogenase pa3128 from pseudomonas aeruginosa pao1 in complex with NADP+
59% identity, 98% coverage: 6:249/250 of query aligns to 6:249/250 of 8bcjB
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G11), S13 (= S13), R14 (= R14), G15 (= G15), I16 (= I16), L36 (= L36), R37 (≠ Q37), N38 (≠ D38), A61 (= A61), D62 (= D62), V63 (= V63), N89 (= N89), A90 (= A90), G91 (= G91), T113 (≠ V113), V143 (= V143), S145 (= S145), Y159 (= Y159), K163 (= K163), P189 (= P189), G190 (= G190), I192 (= I192), T194 (= T194), I196 (≠ M196), H197 (= H197)
4iqgD Crystal structure of bpro0239 oxidoreductase from polaromonas sp. Js666 in NADP bound form
56% identity, 98% coverage: 4:249/250 of query aligns to 2:247/248 of 4iqgD
- active site: G13 (= G15), N112 (= N114), S143 (= S145), Y154 (= Y156), Y157 (= Y159), K161 (= K163)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G11), S11 (= S13), R12 (= R14), G13 (= G15), I14 (= I16), N32 (= N34), A34 (≠ L36), S35 (≠ Q37), N36 (≠ D38), A59 (= A61), D60 (= D62), V61 (= V63), N87 (= N89), A88 (= A90), G89 (= G91), V141 (= V143), S143 (= S145), Y157 (= Y159), K161 (= K163), P187 (= P189), G188 (= G190), I190 (= I192), T192 (= T194), I194 (≠ M196), H195 (= H197)
7v0hG Crystal structure of putative glucose 1-dehydrogenase from burkholderia cenocepacia in complex with NADP and a potential reaction product
38% identity, 98% coverage: 5:250/250 of query aligns to 12:252/253 of 7v0hG
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G11), S20 (= S13), K21 (≠ R14), G22 (= G15), I23 (= I16), A43 (≠ L36), S44 (≠ Q37), S45 (≠ D38), G68 (≠ A61), D69 (= D62), V70 (= V63), N96 (= N89), S97 (≠ A90), G98 (= G91), Y100 (≠ L93), I144 (≠ V143), S146 (= S145), Y159 (= Y159), K163 (= K163), P189 (= P189), G190 (= G190), M191 (≠ L191), I192 (= I192), T194 (= T194), G196 (≠ M196), T197 (≠ H197)
- binding (2R)-2-(hydroxymethyl)pentanedioic acid: S146 (= S145), Y159 (= Y159), M191 (≠ L191), I202 (vs. gap)
9clyB Crystal structure of the 3-ketoacyl-(acyl-carrier-protein) reductase, cylg, from streptococcus agalactiae 2603v/r
37% identity, 98% coverage: 5:250/250 of query aligns to 4:239/242 of 9clyB
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G10 (= G11), R13 (= R14), G14 (= G15), I15 (= I16), Y34 (= Y35), N35 (≠ L36), S36 (≠ Q37), C56 (≠ A61), N57 (≠ D62), I58 (≠ V63), N84 (= N89), I135 (≠ V143), S137 (= S145), Y150 (= Y159), K154 (= K163), G181 (= G190), I183 (= I192), T185 (= T194), M187 (= M196)
3i3oA 2.06 angstrom resolution crystal structure of a short chain dehydrogenase from bacillus anthracis str. 'Ames ancestor' in complex with NAD-acetone
33% identity, 99% coverage: 3:250/250 of query aligns to 37:278/282 of 3i3oA
- active site: G49 (= G15), S174 (= S145), L184 (≠ Y156), Y187 (= Y159), K191 (= K163), K232 (≠ N204)
- binding magnesium ion: D47 (≠ S13), S48 (≠ R14), E72 (≠ D38)
- binding nicotinamide adenine dinucleotide acetone adduct: G45 (= G11), D47 (≠ S13), S48 (≠ R14), G49 (= G15), I50 (= I16), Y69 (= Y35), L70 (= L36), E72 (≠ D38), G95 (≠ A61), D96 (= D62), L97 (≠ V63), N123 (= N89), V124 (≠ A90), A125 (≠ G91), Q126 (≠ I92), Q127 (≠ L93), I147 (≠ V113), T172 (≠ V143), S174 (= S145), Y187 (= Y159), K191 (= K163), P217 (= P189), G218 (= G190), I220 (= I192), T222 (= T194), L224 (≠ M196)
3ijrF 2.05 angstrom resolution crystal structure of a short chain dehydrogenase from bacillus anthracis str. 'Ames ancestor' in complex with NAD+
33% identity, 99% coverage: 3:250/250 of query aligns to 45:286/290 of 3ijrF
- active site: G57 (= G15), S182 (= S145), L192 (≠ Y156), Y195 (= Y159), K199 (= K163), K240 (≠ N204)
- binding magnesium ion: D55 (≠ S13), S56 (≠ R14), E80 (≠ D38)
- binding nicotinamide-adenine-dinucleotide: D55 (≠ S13), S56 (≠ R14), G57 (= G15), I58 (= I16), Y77 (= Y35), L78 (= L36), E80 (≠ D38), G103 (≠ A61), D104 (= D62), L105 (≠ V63), N131 (= N89), V132 (≠ A90), A133 (≠ G91), Q134 (≠ I92), I155 (≠ V113), T180 (≠ V143), S182 (= S145), Y195 (= Y159), K199 (= K163), P225 (= P189), G226 (= G190), P227 (≠ L191), I228 (= I192), T230 (= T194), L232 (≠ M196)
Sites not aligning to the query:
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
35% identity, 100% coverage: 1:250/250 of query aligns to 1:244/246 of 3osuA
P40288 Glucose 1-dehydrogenase; EC 1.1.1.47 from Priestia megaterium (Bacillus megaterium) (see 2 papers)
38% identity, 98% coverage: 5:250/250 of query aligns to 8:250/261 of P40288
- 11:35 (vs. 8:32, 36% identical) binding NADP(+)
- E96 (≠ R94) mutation E->A,G,K: Heat stable.
- D108 (≠ R106) mutation to N: Heat stable.
- V112 (= V110) mutation to A: Heat stable.
- E133 (≠ T131) mutation to K: Heat stable.
- V183 (= V184) mutation to I: Heat stable.
- P194 (≠ E195) mutation to Q: Heat stable.
- E210 (= E210) mutation to K: Heat stable.
- Y217 (≠ R217) mutation to H: Heat stable.
Sites not aligning to the query:
- 252 Q→L: Heat stable.
- 253 Y→C: Heat stable.
- 258 A→G: Heat stable.
1g6kA Crystal structure of glucose dehydrogenase mutant e96a complexed with NAD+
38% identity, 98% coverage: 5:250/250 of query aligns to 8:250/261 of 1g6kA
- active site: G18 (= G15), S145 (= S145), Y158 (= Y159), K162 (= K163)
- binding nicotinamide-adenine-dinucleotide: T17 (≠ R14), G18 (= G15), L19 (≠ I16), R39 (≠ L36), D65 (= D62), V66 (= V63), N92 (= N89), A93 (= A90), G94 (= G91), M143 (≠ V143), S145 (= S145), Y158 (= Y159), P188 (= P189), G189 (= G190), I191 (= I192), T193 (= T194)
3ay6B Crystal structure of bacillus megaterium glucose dehydrogenase 4 a258f mutant in complex with nadh and d-glucose (see paper)
36% identity, 99% coverage: 3:250/250 of query aligns to 12:256/267 of 3ay6B
- active site: G24 (= G15), S151 (= S145), Y164 (= Y159), K168 (= K163)
- binding beta-D-glucopyranose: E102 (≠ R94), S151 (= S145), H153 (≠ A147), W158 (≠ S152), Y164 (= Y159), N202 (≠ H197), K205 (≠ G200)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G11), T23 (≠ R14), G24 (= G15), L25 (≠ I16), Y45 (≠ L36), D71 (= D62), V72 (= V63), N98 (= N89), A99 (= A90), G100 (= G91), V101 (≠ I92), M149 (≠ V143), S151 (= S145), Y164 (= Y159), K168 (= K163), P194 (= P189), G195 (= G190), M197 (≠ I192), T199 (= T194), P200 (≠ E195), I201 (≠ M196), N202 (≠ H197)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
35% identity, 98% coverage: 5:250/250 of query aligns to 2:237/239 of 3sj7A
- active site: G12 (= G15), S138 (= S145), Q148 (≠ E155), Y151 (= Y159), K155 (= K163)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), S10 (= S13), R11 (= R14), I13 (= I16), N31 (= N34), Y32 (= Y35), A33 (≠ L36), G34 (≠ Q37), S35 (≠ D38), A58 (= A61), N59 (≠ D62), V60 (= V63), N86 (= N89), A87 (= A90), T109 (≠ V113), S138 (= S145), Y151 (= Y159), K155 (= K163), P181 (= P189), G182 (= G190)
4iinA Crystal structure of a putative 3-oxoacyl-[acyl-carrier protein]reductase from helicobacter pylori 26695 complexed with NAD+
37% identity, 98% coverage: 5:250/250 of query aligns to 7:234/236 of 4iinA
- active site: G17 (= G15), S143 (= S145), Y156 (= Y159), K160 (= K163)
- binding nicotinamide-adenine-dinucleotide: G13 (= G11), G17 (= G15), I18 (= I16), N36 (= N34), R38 (≠ L36), A65 (≠ V63), N91 (= N89), A92 (= A90), G93 (= G91), V141 (= V143), S143 (= S145), Y156 (= Y159), K160 (= K163), P186 (= P189)
8w0oA Gdh-105 crystal structure
34% identity, 99% coverage: 3:250/250 of query aligns to 6:250/259 of 8w0oA
- binding nicotinamide-adenine-dinucleotide: G14 (= G11), S17 (≠ R14), G18 (= G15), L19 (≠ I16), Y39 (≠ L36), Q43 (≠ A40), D65 (= D62), V66 (= V63), N92 (= N89), A93 (= A90), G94 (= G91), M143 (≠ V143), Y158 (= Y159), K162 (= K163), P188 (= P189), G189 (= G190), I191 (= I192), T193 (= T194)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
34% identity, 99% coverage: 3:250/250 of query aligns to 4:245/247 of 4jroC
- active site: G16 (= G15), S142 (= S145), Q152 (≠ E155), Y155 (= Y159), K159 (= K163)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), S14 (= S13), R15 (= R14), G16 (= G15), I17 (= I16), N35 (= N34), Y36 (= Y35), N37 (≠ L36), G38 (≠ Q37), S39 (≠ D38), N63 (≠ D62), V64 (= V63), N90 (= N89), A91 (= A90), I93 (= I92), I113 (≠ V113), S142 (= S145), Y155 (= Y159), K159 (= K163), P185 (= P189), I188 (= I192), T190 (= T194)
4cqmF Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD and NADP (see paper)
38% identity, 99% coverage: 4:250/250 of query aligns to 6:237/241 of 4cqmF
- active site: G17 (= G15), S139 (= S145), Q149 (≠ E155), Y152 (= Y159), K156 (= K163)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G11), R16 (= R14), G17 (= G15), I18 (= I16), A37 (≠ V33), R38 (≠ N34), N39 (≠ Y35), D60 (= D62), V61 (= V63), A87 (≠ N89), A88 (= A90), G89 (= G91), V137 (= V143), S139 (= S145), Y152 (= Y159), K156 (= K163), V185 (≠ I192), T187 (= T194), M189 (= M196)
8jfgA Crystal structure of 3-oxoacyl-acp reductase fabg in complex with NADP+ and 3-keto-octanoyl-acp from helicobacter pylori (see paper)
36% identity, 98% coverage: 5:250/250 of query aligns to 7:246/248 of 8jfgA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G11), K16 (≠ R14), I18 (= I16), R38 (≠ L36), A65 (≠ V63), N91 (= N89), A92 (= A90), G93 (= G91), A142 (≠ S144), S143 (= S145), Y156 (= Y159), K160 (= K163), P186 (= P189), I189 (= I192), T191 (= T194), M193 (= M196), N194 (≠ H197)
- binding ~{S}-[2-[3-[[(2~{S})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] 3-oxidanylideneoctanethioate: D97 (≠ Q95), S143 (= S145), I145 (≠ A147), M151 (≠ P153), G152 (≠ N154), Q153 (≠ E155), Y156 (= Y159), G187 (= G190), F188 (≠ L191), Y205 (≠ R208)
Q8N4T8 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-[acyl-carrier-protein] reductase beta subunit; KAR beta subunit; Carbonyl reductase family member 4; CBR4; Quinone reductase CBR4; Short chain dehydrogenase/reductase family 45C member 1; EC 1.1.1.100; EC 1.6.5.10 from Homo sapiens (Human) (see 4 papers)
38% identity, 99% coverage: 4:250/250 of query aligns to 2:233/237 of Q8N4T8
- G9 (= G11) mutation to S: Unable to restore growth of an OAR1-deficient yeast mutant.
- SRGI 11:14 (= SRGI 13:16) binding NADP(+)
- R12 (= R14) mutation to A: Strongly reduced ability to restore growth of an OAR1-deficient yeast mutant.
- R34 (≠ N34) mutation to A: Strongly reduced ability to restore growth of an OAR1-deficient yeast mutant. Strongly reduces NADPH-dependent reductase activity with acetoacetyl-CoA and 9,10-phenanthrene quinone. No effect on NADH-dependent reductase activities.
- RN 34:35 (≠ NY 34:35) binding NADP(+)
- D56 (= D62) binding NADP(+)
- L70 (≠ V76) to M: in dbSNP:rs2877380
- AAG 83:85 (≠ NAG 89:91) binding NADP(+)
- S135 (= S145) mutation to A: Unable to restore growth of an OAR1-deficient yeast mutant.
- Y148 (= Y159) binding NADP(+); mutation to A: Unable to restore growth of an OAR1-deficient yeast mutant.
- K152 (= K163) binding NADP(+); mutation to A: Unable to restore growth of an OAR1-deficient yeast mutant. Abolishes NADPH-dependent reductase activity with acetoacetyl-CoA. Strongly reduces NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on NADH-dependent reductase activities.
- R168 (≠ A179) mutation to E: Strongly reduced ability to restore growth of an OAR1-deficient yeast mutant. Increases NADPH-dependent reductase activity with acetoacetyl-CoA. Reduces NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on NADH-dependent reductase activities.
- K169 (≠ E180) mutation to E: Unable to restore growth of an OAR1-deficient yeast mutant. Increases NADPH-dependent reductase activity with acetoacetyl-CoA. Reduces NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on NADH-dependent reductase activities.
- VHT 181:183 (≠ IYT 192:194) binding NADP(+)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
37% identity, 98% coverage: 5:250/250 of query aligns to 7:244/247 of P73574
- A14 (≠ G12) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (= P153) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K163) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ L191) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ R205) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
3r3sA Structure of the ygha oxidoreductase from salmonella enterica
33% identity, 95% coverage: 3:240/250 of query aligns to 46:279/292 of 3r3sA
- active site: G58 (= G15), S184 (= S145), L194 (≠ Y156), Y197 (= Y159), K201 (= K163), Q242 (vs. gap)
- binding magnesium ion: D56 (≠ S13), S57 (≠ R14), E82 (≠ D38)
- binding nicotinamide-adenine-dinucleotide: D56 (≠ S13), S57 (≠ R14), G58 (= G15), I59 (= I16), L79 (= L36), E82 (≠ D38), D106 (= D62), L107 (≠ V63), V133 (≠ N89), A134 (= A90), G135 (= G91), S184 (= S145), Y197 (= Y159), K201 (= K163), P227 (= P189), G228 (= G190), I230 (= I192), T232 (= T194), L234 (≠ M196), Q235 (≠ H197)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
33% identity, 100% coverage: 1:250/250 of query aligns to 2:242/244 of 4nbuB
- active site: G18 (= G15), N111 (= N114), S139 (= S145), Q149 (≠ E155), Y152 (= Y159), K156 (= K163)
- binding acetoacetyl-coenzyme a: D93 (≠ Q95), K98 (≠ E101), S139 (= S145), N146 (≠ S152), V147 (≠ P153), Q149 (≠ E155), Y152 (= Y159), F184 (≠ L191), M189 (= M196), K200 (≠ R208)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G11), N17 (≠ R14), G18 (= G15), I19 (= I16), D38 (≠ Y35), F39 (≠ L36), V59 (≠ A61), D60 (= D62), V61 (= V63), N87 (= N89), A88 (= A90), G89 (= G91), I90 (= I92), T137 (≠ V143), S139 (= S145), Y152 (= Y159), K156 (= K163), P182 (= P189), F184 (≠ L191), T185 (≠ I192), T187 (= T194), M189 (= M196)
Query Sequence
>WP_015820561.1 NCBI__GCF_000023025.1:WP_015820561.1
MSRNKVAIITGGSRGIGAETAKLLALNGYAVCVNYLQDDAAAQSLKREISDMDVKCIAVK
ADVSRFSDVSRLFETVDKELGSLSVLVNNAGILRQQCRLDEISEDRFSEVLRVNVMGCFL
CCKEAVKRMSTKYGGVGGAIVNVSSRASVTGSPNEYVDYAASKGAIDTLTRGLALEVAAE
GIRVNGVRPGLIYTEMHASGGESNRVKRLENKIPMQRGGEPSEVAEAIYWLVSNKSAFVT
GSFIEPSGGL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory