SitesBLAST
Comparing WP_015887793.1 NCBI__GCF_000018545.1:WP_015887793.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3bfjA Crystal structure analysis of 1,3-propanediol oxidoreductase (see paper)
30% identity, 99% coverage: 6:377/377 of query aligns to 1:382/382 of 3bfjA
P31005 NAD-dependent methanol dehydrogenase; MDH; MEDH; Type 3 alcohol dehydrogenase; EC 1.1.1.244 from Bacillus methanolicus (see 3 papers)
31% identity, 95% coverage: 18:377/377 of query aligns to 12:381/381 of P31005
- G13 (= G19) mutation to A: Shows a reduced dehydrogenase activity.
- G15 (= G21) mutation to A: Shows almost the same dehydrogenase activity as the wild-type.
- D88 (= D87) mutation to N: Shows almost the same dehydrogenase activity as the wild-type.
- G95 (= G94) mutation to A: Shows a 10-fold decreased affinity for NAD and NADH and a strongly reduced dehydrogenase activity. Completely insensitive to the stimulating effect of the activator protein Act.
- S97 (= S96) mutation to G: Shows an increase of the dehydrogenase activity and a decrease of the affinity for NAD and NADH. Completely insensitive to the stimulating effect of the activator protein Act. It does not bind NAD.; mutation to T: Shows an increase of the dehydrogenase activity and affinity for NAD and NADH.
- D100 (= D99) mutation to N: Loss of dehydrogenase activity. It still binds NADH.
- K103 (= K102) mutation to R: Loss of dehydrogenase activity. It does not bind NADH.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
P0DJA2 Alcohol dehydrogenase 2; Alcohol dehydrogenase II; ADH II; EC 1.1.1.1 from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) (see 2 papers)
30% identity, 97% coverage: 13:377/377 of query aligns to 9:383/383 of P0DJA2
- D39 (= D43) binding NAD(+)
- N71 (≠ E68) binding NAD(+)
- G98 (= G95) binding NAD(+)
- S99 (= S96) binding NAD(+)
- T138 (= T136) binding NAD(+)
- T139 (= T137) binding NAD(+)
- T147 (≠ G145) binding NAD(+)
- F149 (≠ R147) binding NAD(+)
- K160 (= K158) binding NAD(+)
- L179 (= L177) binding NAD(+)
- G182 (≠ T180) binding NAD(+)
- M183 (≠ V181) binding NAD(+)
- D194 (= D192) binding Fe(2+)
- H198 (= H196) binding Fe(2+)
- H263 (= H261) binding Fe(2+)
- H267 (≠ Y265) binding NAD(+)
- H277 (= H275) binding Fe(2+); binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3ox4A Structures of iron-dependent alcohol dehydrogenase 2 from zymomonas mobilis zm4 complexed with NAD cofactor (see paper)
30% identity, 97% coverage: 13:377/377 of query aligns to 8:382/382 of 3ox4A
- binding fe (ii) ion: D193 (= D192), H197 (= H196), H262 (= H261), H276 (= H275)
- binding nicotinamide-adenine-dinucleotide: D38 (= D43), F40 (= F45), M41 (vs. gap), N70 (≠ E68), G96 (= G94), G97 (= G95), S98 (= S96), T137 (= T136), T138 (= T137), F148 (≠ R147), I150 (≠ L149), G181 (≠ T180), M182 (≠ V181), L186 (≠ V185), H276 (= H275)
3owoA Structures of iron-dependent alcohol dehydrogenase 2 from zymomonas mobilis zm4 with and without NAD cofactor (see paper)
30% identity, 97% coverage: 13:377/377 of query aligns to 8:382/382 of 3owoA
6jkpA Crystal structure of sulfoacetaldehyde reductase from bifidobacterium kashiwanohense in complex with NAD+ (see paper)
28% identity, 96% coverage: 12:374/377 of query aligns to 8:370/376 of 6jkpA
- binding nicotinamide-adenine-dinucleotide: F42 (≠ N46), G96 (= G95), D100 (= D99), T137 (= T136), T138 (= T137), T141 (= T140), S143 (= S142), T146 (≠ G145), S181 (≠ T180), V182 (= V181), P183 (= P182)
6jkoA Crystal structure of sulfoacetaldehyde reductase from bifidobacterium kashiwanohense (see paper)
28% identity, 96% coverage: 12:374/377 of query aligns to 8:370/376 of 6jkoA
5yvmA Crystal structure of the archaeal halo-thermophilic red sea brine pool alcohol dehydrogenase adh/d1 bound to nzq (see paper)
31% identity, 96% coverage: 16:377/377 of query aligns to 15:403/403 of 5yvmA
- binding manganese (ii) ion: D207 (= D192), H211 (= H196), H276 (= H261), H291 (= H275)
- binding 5,6-dihydroxy-nadp: G41 (≠ S42), N44 (vs. gap), M45 (vs. gap), P73 (≠ A67), N74 (≠ E68), G100 (= G94), G101 (= G95), S102 (= S96), D105 (= D99), S151 (≠ T136), T152 (= T137), T155 (= T140), T160 (≠ G145), Y162 (≠ R147), V164 (≠ L149), K173 (= K158), E195 (≠ T180), L200 (≠ V185), H211 (= H196), H276 (= H261), H280 (≠ Y265), H291 (= H275)
5yvrA Crystal structure of the h277a mutant of adh/d1, an archaeal halo- thermophilic red sea brine pool alcohol dehydrogenase (see paper)
31% identity, 96% coverage: 16:377/377 of query aligns to 15:403/403 of 5yvrA
- binding manganese (ii) ion: D207 (= D192), H211 (= H196), H276 (= H261), H291 (= H275)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G41 (≠ S42), S43 (≠ A44), N44 (vs. gap), M45 (vs. gap), G100 (= G94), G101 (= G95), S102 (= S96), D105 (= D99), S151 (≠ T136), T152 (= T137), T155 (= T140), T160 (≠ G145), Y162 (≠ R147), V164 (≠ L149), K173 (= K158), E195 (≠ T180), M196 (≠ V181), L200 (≠ V185), D207 (= D192), H211 (= H196), H291 (= H275)
1vhdA Crystal structure of an iron containing alcohol dehydrogenase (see paper)
30% identity, 97% coverage: 13:377/377 of query aligns to 8:358/361 of 1vhdA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S39 (vs. gap), S40 (= S42), E69 (≠ D70), N70 (≠ T71), G96 (= G94), G97 (= G95), S98 (= S96), D101 (= D99), T137 (= T136), T138 (= T137), T141 (= T140), S143 (= S142), T146 (≠ G145), Y148 (≠ R147), I150 (≠ L149), K158 (≠ A157), S178 (≠ T180), M179 (≠ V181), L183 (≠ V185), D190 (= D192), H194 (= H196), H271 (= H275)
- binding zinc ion: D190 (= D192), H194 (= H196), H257 (= H261), H271 (= H275)
1o2dA Crystal structure of alcohol dehydrogenase, iron-containing (tm0920) from thermotoga maritima at 1.30 a resolution (see paper)
30% identity, 97% coverage: 13:377/377 of query aligns to 7:357/359 of 1o2dA
- binding fe (iii) ion: D189 (= D192), H193 (= H196), H256 (= H261), H270 (= H275)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S38 (vs. gap), S39 (= S42), E68 (≠ D70), N69 (≠ T71), G95 (= G94), G96 (= G95), S97 (= S96), D100 (= D99), T136 (= T136), T137 (= T137), T140 (= T140), S142 (= S142), Y147 (≠ R147), I149 (≠ L149), K157 (≠ A157), S177 (≠ T180), M178 (≠ V181), L182 (≠ V185), D189 (= D192), H193 (= H196), H270 (= H275)
3zdrA Structure of the alcohol dehydrogenase (adh) domain of a bifunctional adhe dehydrogenase from geobacillus thermoglucosidasius ncimb 11955 (see paper)
30% identity, 83% coverage: 66:377/377 of query aligns to 65:400/403 of 3zdrA