SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_015888109.1 NCBI__GCF_000018545.1:WP_015888109.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8i01A Crystal structure of escherichia coli glyoxylate carboligase (see paper)
74% identity, 99% coverage: 1:587/594 of query aligns to 2:586/594 of 8i01A

query
sites
8i01A

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binding flavin-adenine dinucleotide: R155 (= R154), G212 (= G211), G213 (= G212), G214 (= G213), N217 (= N216), T238 (= T237), L239 (= L238), M240 (= M239), V256 (≠ C255), G257 (= G256), Q259 (= Q258), T260 (= T259), G279 (= G278), N280 (= N279), R281 (= R280), A283 (= A282), R285 (= R284), H286 (= H285), D303 (= D302), I304 (= I303), Q308 (= Q307), D322 (= D321), A323 (= A322), G417 (= G416)
binding magnesium ion: D447 (= D446), F452 (= F451), E455 (= E454), N474 (= N473), Y476 (= Y475)
binding thiamine diphosphate: G395 (= G394), L396 (= L395), S397 (= S396), L422 (= L421), G446 (= G445), D447 (= D446), F448 (≠ Y447), D449 (= D448), N474 (= N473), Y476 (= Y475), L477 (= L476), G478 (= G477), L479 (= L478), I480 (= I479)
binding 2,3-dimethoxy-5-methyl-1,4-benzoquinone: Q354 (≠ R353), C492 (≠ E491), Q494 (≠ S493)

Sites not aligning to the query:

binding 2,3-dimethoxy-5-methyl-1,4-benzoquinone: 589

8beoB Crystal structure of e. Coli glyoxylate carboligase mutant i393a with map
74% identity, 99% coverage: 2:587/594 of query aligns to 1:584/592 of 8beoB

query
sites
8beoB

A

A

K

K

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M

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I

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S

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L

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E

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R

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T

N

N

I

I

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M

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G

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A

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T

T

binding methyl hydrogen (s)-acetylphosphonate: C490 (≠ E491), Q492 (≠ S493)
binding flavin-adenine dinucleotide: R153 (= R154), P154 (= P155), G210 (= G211), G211 (= G212), G212 (= G213), N215 (= N216), T236 (= T237), L237 (= L238), M238 (= M239), V254 (≠ C255), G255 (= G256), Q257 (= Q258), T258 (= T259), G277 (= G278), N278 (= N279), R279 (= R280), A281 (= A282), R283 (= R284), H284 (= H285), D301 (= D302), I302 (= I303), Q306 (= Q307), D320 (= D321), A321 (= A322), G415 (= G416)
binding magnesium ion: D445 (= D446), F450 (= F451), L451 (= L452), E453 (= E454), N472 (= N473), Y474 (= Y475)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(1s)-1-hydroxy-1-[(r)-hydroxy(methoxy)phosphoryl]ethyl}-5-(2-{[(s)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: R283 (= R284), G393 (= G394), L394 (= L395), S395 (= S396), L420 (= L421), G444 (= G445), D445 (= D446), F446 (≠ Y447), D447 (= D448), N472 (= N473), Y474 (= Y475), L475 (= L476), G476 (= G477), L477 (= L478), I478 (= I479)
binding 2,3-dimethoxy-5-methyl-1,4-benzoquinone: Q352 (≠ R353), R356 (= R357)

Sites not aligning to the query:

binding 2,3-dimethoxy-5-methyl-1,4-benzoquinone: 587

8beoA Crystal structure of e. Coli glyoxylate carboligase mutant i393a with map
74% identity, 99% coverage: 2:587/594 of query aligns to 1:584/592 of 8beoA

query
sites
8beoA

A

A

K

K

M

M

R

R

A

A

V

V

D

D

A

A

A

A

V

M

H

Y

V

V

L

L

E

E

K

K

E

E

G

G

V

I

D

T

C

T

A

A

F

F

G

G

V

V

P

P

G

G

A

A

A

A

I

I

N

N

P

P

L

F

Y

Y

S

S

A

A

L

M

R

R

A

K

R

H

G

G

S

G

I

I

R

R

H

H

V

I

L

L

A

A

R

R

H

H

V

V

E

E

G

G

A

A

S

S

H

H

M

M

A

A

E

E

G

G

Y

Y

T

T

R

R

A

A

K

T

H

A

G

G

N

N

I

I

G

G

I

V

C

C

I

L

G

G

T

T

S

S

G

G

P

P

A

A

G

G

T

T

D

D

M

M

I

I

T

T

G

A

L

L

Y

Y

S

S

A

A

S

S

A

A

D

D

S

S

I

I

P

P

I

I

L

L

C

C

I

I

T

T

G

G

Q

Q

A

A

P

P

R

R

A

A

R

R

L

L

D

H

K

K

E

E

D

D

F

F

Q

Q

A

A

V

V

D

D

I

I

A

E

A

A

I

I

A

A

G

K

P

P

V

V

T

S

K

K

W

M

A

A

V

V

T

T

V

V

M

R

E

E

P

A

A

A

L

L

V

V

P

P

F

R

V

V

F

L

Q

Q

K

Q

A

A

F

F

H

H

L

L

M

M

R

R

S

S

G

G

R
|
R

P
|
P

G

G

P

P

V

V

L

L

I

V

D

D

L

L

P

P

V

F

D

D

V

V

Q

Q

L

V

A

A

E

E

I

I

E

E

F

F

D

D

P

P

E

D

T

M

Y

Y

E

E

P

P

L

L

A

P

P

V

Y

Y

K

K

P

P

A

A

A

A

T

S

R

R

A

M

Q

Q

A

I

E

E

K

K

A

A

L

V

A

E

M

M

L

L

N

I

A

Q

S

A

E

E

R

R

P

P

L

V

I

I

V

V

A

A

G
|
G

G
|
G

G
|
G

I

V

I

I

N
|
N

A

A

D

D

A

A

S

A

D

A

L

L

L

L

T

Q

E

Q

F

F

A

A

E

E

I

L

T

T

G

S

V

V

P

P

V

V

I

I

P

P

T
|
T

L
|
L

M
|
M

G

G

W

W

G

G

T

C

I

I

P

P

D

D

D

D

H

H

P
x
E

L

L

M

M

A

A

G

G

M

M

C
x
V

G
|
G

L

L

Q
|
Q

T
|
T

S

A

H

H

R

R

Y

Y

G

G

N

N

A

A

T

T

L

L

L

L

A

A

S

S

D

D

F

M

V

V

F

F

G

G

I

I

G
|
G

N
|
N

R
|
R

W

F

A
|
A

N

N

R
|
R

H
|
H

T

T

G

G

N

S

V

V

P

E

T

K

Y

Y

T

T

E

E

G

G

R

R

K

K

F

I

I

V

H

H

V

I

D
|
D

I
|
I

E

E

P

P

T

T

Q
|
Q

I

I

G

G

R

R

V

V

F

L

A

C

P

P

D

D

F

L

G

G

I

I

V

V

S

S

D
|
D

A
|
A

A

K

A

A

A

A

L

L

K

T

L

L

F

L

L

V

D

E

V

V

A

A

T

Q

E

E

W

M

K

Q

T

K

A

A

G

G

K

R

L

L

R

P

D

C

W

R

S

K

A

E

W

W

A

V

D

A

E

D

C

C

R
x
Q

E

Q

R

R

K

K

R

R

T

T

M

L

L

L

R

R

K

K

T

T

H

H

F

F

D

D

Q

N

T

V

P

P

L

V

K

K

P

P

Q

Q

R

R

V

V

Y

Y

E

E

E

E

M

M

N

N

K

K

A

A

F

F

G

G

R
|
R

D

D

T

V

C

C

F

Y

V

V

S

T

T

T

I

A

G
|
G

L
|
L

S
|
S

Q

Q

I
|
I

A

A

G

A

A

A

Q

Q

F

M

L

L

H

H

V
|
V

Y
x
F

R

K

P

D

R

R

N
x
H

W

W

I

I

N

N

C

C

G
|
G

Q

Q

A

A

G

G

P

P

L
|
L

G

G

W

W

T

T

L

I

P

P

A

A

A

A

L

L

G

G

V

V

R

C

A

A

A

A

D

D

P

P

N

K

R

R

P

N

I

V

V

V

A

A

L

I

S

S

G
|
G

D
|
D

Y
x
F

D
|
D

F

F

Q

Q

F
|
F

L
|
L

I

I

E
|
E

E

E

L

L

A

A

V

V

G

G

A

A

Q

Q

H

F

K

N

L

I

P

P

Y

Y

L

I

H

H

V

V

V

L

V

V

N

N

N
|
N

S

A

Y
|
Y

L
|
L

G
|
G

L
|
L

I
|
I

R

R

Q

Q

A

S

Q

Q

R

R

G

A

F

F

E

D

M

M

D

D

F

Y

E

C

V

V

S

Q

L

L

A

A

F

F

D

E

N

N

I

I

N

N

A

S

S

S

G

-

D

-

A

E

E

V

K

N

G

G

Y

Y

G

G

V

V

D

D

H

H

V

V

A

K

V

V

A

A

E

E

G

G

L

L

G

G

C

C

K

K

A

A

I

I

R

R

V

V

K

F

S

K

P

P

N

E

E

D

F

I

A

A

D

P

A

A

F

F

E

E

R

Q

A

A

Q

K

A

A

L

L

M

M

E

A

E

Q

H

Y

R

R

V

V

P

P

V

V

V

V

I

V

E

E

F

V

I

I

L

L

E

E

R

R

V

V

T

T

N

N

I

I

A

S

M

M

G

G

A

S

D

E

I

L

N

D

A

N

V

V

V

M

E

E

F

F

E

E

E

D

L

I

A

A

A

D

R

N

G

A

E

A

D

D

A

A

P

P

T

T

binding flavin-adenine dinucleotide: R153 (= R154), P154 (= P155), G210 (= G211), G211 (= G212), G212 (= G213), N215 (= N216), T236 (= T237), L237 (= L238), M238 (= M239), V254 (≠ C255), G255 (= G256), Q257 (= Q258), T258 (= T259), G277 (= G278), N278 (= N279), R279 (= R280), A281 (= A282), R283 (= R284), H284 (= H285), D301 (= D302), I302 (= I303), Q306 (= Q307), D320 (= D321), A321 (= A322), I397 (= I398), G415 (= G416)
binding magnesium ion: R384 (= R385), V405 (= V406), F406 (≠ Y407), H410 (≠ N411), D445 (= D446), F450 (= F451), L451 (= L452), E453 (= E454), N472 (= N473), Y474 (= Y475)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(1s)-1-hydroxy-1-[(r)-hydroxy(methoxy)phosphoryl]ethyl}-5-(2-{[(s)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: R283 (= R284), G393 (= G394), L394 (= L395), S395 (= S396), L420 (= L421), G444 (= G445), D445 (= D446), F446 (≠ Y447), D447 (= D448), N472 (= N473), Y474 (= Y475), L475 (= L476), G476 (= G477), L477 (= L478), I478 (= I479)
binding 2,3-dimethoxy-5-methyl-1,4-benzoquinone: E248 (≠ P249), Q352 (≠ R353)

Sites not aligning to the query:

binding 2,3-dimethoxy-5-methyl-1,4-benzoquinone: 587

8i07D Crystal structure of escherichia coli glyoxylate carboligase double mutant in complex with glycolaldehyde (see paper)
74% identity, 99% coverage: 1:587/594 of query aligns to 2:586/594 of 8i07D

query
sites
8i07D

M

M

A

A

K

K

M

M

R

R

A

A

V

V

D

D

A

A

A

A

V

M

H

Y

V

V

L

L

E

E

K

K

E

E

G

G

V

I

D

T

C

T

A

A

F

F

G

G

V

V

P

P

G

G

A

A

A

A

I

I

N

N

P

P

L

F

Y

Y

S

S

A

A

L

M

R

R

A

K

R

H

G

G

S

G

I

I

R

R

H

H

V

I

L

L

A

A

R

R

H

H

V
|
V

E

E

G

G

A

A

S

S

H

H

M

M

A

A

E

E

G

G

Y

Y

T

T

R

R

A

A

K

T

H

A

G

G

N

N

I

I

G

G

I

V

C

C

I

L

G

G

T
|
T

S

S

G

G

P

P

A

A

G

G

T

T

D

D

M

M

I

I

T

T

G

A

L

L

Y

Y

S

S

A

A

S

S

A

A

D

D

S

S

I

I

P

P

I

I

L

L

C

C

I

I

T

T

G

G

Q

Q

A

A

P

P

R

R

A

A

R

R

L

L

D

H

K

K

E

E

D

D

F

F

Q

Q

A

A

V

V

D

D

I

I

A

E

A

A

I

I

A

A

G

K

P

P

V

V

T

S

K

K

W

M

A

A

V

V

T

T

V

V

M

R

E

E

P

A

A

A

L

L

V

V

P

P

F

R

V

V

F

L

Q

Q

K

Q

A

A

F

F

H

H

L

L

M

M

R

R

S

S

G

G

R
|
R

P
|
P

G

G

P

P

V

V

L

L

I

V

D

D

L

L

P

P

V

F

D

D

V

V

Q

Q

L

V

A

A

E

E

I

I

E

E

F

F

D

D

P

P

E

D

T

M

Y

Y

E

E

P

P

L

L

A

P

P

V

Y

Y

K

K

P

P

A

A

A

A

T

S

R

R

A

M

Q

Q

A

I

E

E

K

K

A

A

L

V

A

E

M

M

L

L

N

I

A

Q

S

A

E

E

R

R

P

P

L

V

I

I

V

V

A

A

G
|
G

G
|
G

G
|
G

I

V

I

I

N
|
N

A

A

D

D

A

A

S

A

D

A

L

L

L

L

T

Q

E

Q

F

F

A

A

E

E

I

L

T

T

G

S

V

V

P

P

V

V

I

I

P

P

T
|
T

L
|
L

M
|
M

G

G

W

W

G

G

T

C

I

I

P

P

D

D

D

D

H

H

P

E

L

L

M

M

A

A

G

G

M

M

C
x
V

G
|
G

L

L

Q
|
Q

T
|
T

S

A

H

H

R

R

Y

Y

G

G

N

N

A

A

T

T

L

L

L

L

A

A

S

S

D

D

F

M

V

V

F

F

G

G

I

I

G
|
G

N
|
N

R
|
R

W

F

A

A

N

Q

R
|
R

H
|
H

T

T

G

G

N

S

V

V

P

E

T

K

Y

Y

T

T

E

E

G

G

R

R

K

K

F

I

I

V

H

H

V

I

D
|
D

I
|
I

E

E

P

P

T

T

Q
|
Q

I

I

G

G

R

R

V

V

F

L

A

C

P

P

D

D

F

L

G

G

I

I

V

V

S

S

D
|
D

A
|
A

A

K

A

A

A

A

L

L

K

T

L

L

F

L

L

V

D

E

V

V

A

A

T

Q

E

E

W

M

K

Q

T

K

A

A

G

G

K

R

L

L

R

P

D

C

W

R

S

K

A

E

W

W

A

V

D

A

E

D

C

C

R
x
Q

E

Q

R

R

K

K

R
|
R

T

T

M

L

L

L

R

R

K

K

T

T

H

H

F

F

D

D

Q

N

T

V

P

P

L

V

K

K

P

P

Q

Q

R

R

V

V

Y

Y

E

E

E

E

M

M

N

N

K

K

A

A

F

F

G

G

R

R

D

D

T

V

C

C

F

Y

V

V

S

T

T

T

I

I

G
|
G

L
|
L

S
|
S

Q

Q

I
|
I

A

A

G

A

A

A

Q

Q

F

M

L

L

H

H

V

V

Y

F

R

K

P

D

R

R

N

H

W

W

I

I

N

N

C

C

G
|
G

Q

Q

A

A

G

G

P

P

L
|
L

G

G

W

W

T

T

L

I

P

P

A

A

A

A

L

L

G

G

V

V

R

C

A

A

A

A

D

D

P

P

N

K

R

R

P

N

I

V

V

V

A

A

L

I

S

S

G
|
G

D
|
D

Y
x
F

D
|
D

F

F

Q

Q

F
|
F

L
|
L

I

I

E
|
E

E

E

L

L

A

A

V

V

G

G

A

A

Q

Q

H

F

K

N

L

I

P

P

Y

Y

L

I

H

H

V

V

V

L

V

V

N

N

N
|
N

S

A

Y
|
Y

L
|
L

G
|
G

L
|
L

I
|
I

R

R

Q

Q

A

S

Q

Q

R

M

G

A

F

F

E

D

M

M

D

D

F

Y

E
x
C

V

V

S
x
Q

L

L

A

A

F

F

D

E

N

N

I

I

N

N

A

S

S

S

G

-

D

-

A

E

E

V

K

N

G

G

Y

Y

G

G

V

V

D

D

H

H

V

V

A

K

V

V

A

A

E

E

G

G

L

L

G

G

C

C

K

K

A

A

I

I

R

R

V

V

K

F

S

K

P

P

N

E

E

D

F

I

A

A

D

P

A

A

F

F

E

E

R

Q

A

A

Q

K

A

A

L

L

M

M

E

A

E

Q

H

Y

R

R

V

V

P

P

V

V

V

V

I

V

E

E

F

V

I

I

L

L

E

E

R

R

V

V

T

T

N

N

I

I

A

S

M

M

G

G

A

S

D

E

I

L

N

D

A

N

V

V

V

M

E

E

F

F

E

E

E

D

L

I

A

A

A

D

R

N

G

A

E

A

D

D

A

A

P

P

T

T

binding 2-oxidanylethanal: R285 (= R284), I480 (= I479)
binding flavin-adenine dinucleotide: R155 (= R154), P156 (= P155), G212 (= G211), G213 (= G212), G214 (= G213), N217 (= N216), T238 (= T237), L239 (= L238), M240 (= M239), V256 (≠ C255), G257 (= G256), Q259 (= Q258), T260 (= T259), G279 (= G278), N280 (= N279), R281 (= R280), R285 (= R284), H286 (= H285), D303 (= D302), I304 (= I303), Q308 (= Q307), D322 (= D321), A323 (= A322), I399 (= I398), G417 (= G416)
binding magnesium ion: D447 (= D446), F452 (= F451), L453 (= L452), E455 (= E454), N474 (= N473), Y476 (= Y475)
binding thiamine diphosphate: V52 (= V51), T76 (= T75), G395 (= G394), L396 (= L395), S397 (= S396), L422 (= L421), G446 (= G445), D447 (= D446), F448 (≠ Y447), D449 (= D448), N474 (= N473), Y476 (= Y475), L477 (= L476), G478 (= G477), L479 (= L478), I480 (= I479)
binding ubiquinone-1: Q354 (≠ R353), R358 (= R357), C492 (≠ E491), Q494 (≠ S493)

Sites not aligning to the query:

binding ubiquinone-1: 589

8i07A Crystal structure of escherichia coli glyoxylate carboligase double mutant in complex with glycolaldehyde (see paper)
74% identity, 99% coverage: 1:587/594 of query aligns to 2:586/594 of 8i07A

query
sites
8i07A

M

M

A

A

K

K

M

M

R

R

A

A

V

V

D

D

A

A

A

A

V

M

H

Y

V

V

L

L

E

E

K

K

E

E

G

G

V

I

D

T

C

T

A

A

F

F

G

G

V

V

P

P

G

G

A

A

A

A

I

I

N

N

P

P

L

F

Y

Y

S

S

A

A

L

M

R

R

A

K

R

H

G

G

S

G

I

I

R

R

H

H

V

I

L

L

A

A

R

R

H

H

V

V

E

E

G

G

A

A

S

S

H

H

M

M

A

A

E

E

G

G

Y

Y

T

T

R

R

A

A

K

T

H

A

G

G

N

N

I

I

G

G

I

V

C

C

I

L

G

G

T

T

S

S

G

G

P

P

A

A

G

G

T

T

D

D

M

M

I

I

T

T

G

A

L

L

Y

Y

S

S

A

A

S

S

A

A

D

D

S

S

I

I

P

P

I

I

L

L

C

C

I

I

T

T

G

G

Q

Q

A

A

P

P

R

R

A

A

R

R

L

L

D

H

K

K

E

E

D

D

F

F

Q

Q

A

A

V

V

D

D

I

I

A

E

A

A

I

I

A

A

G

K

P

P

V

V

T

S

K

K

W

M

A

A

V

V

T

T

V

V

M

R

E

E

P

A

A

A

L

L

V

V

P

P

F

R

V

V

F

L

Q

Q

K

Q

A

A

F

F

H

H

L

L

M

M

R

R

S

S

G

G

R
|
R

P
|
P

G

G

P

P

V

V

L

L

I

V

D

D

L

L

P

P

V

F

D

D

V

V

Q

Q

L

V

A

A

E

E

I

I

E

E

F

F

D

D

P

P

E

D

T

M

Y

Y

E

E

P

P

L

L

A

P

P

V

Y

Y

K

K

P

P

A

A

A

A

T

S

R

R

A

M

Q

Q

A

I

E

E

K

K

A

A

L

V

A

E

M

M

L

L

N

I

A

Q

S

A

E

E

R

R

P

P

L

V

I

I

V

V

A

A

G
|
G

G
|
G

G
|
G

I

V

I

I

N
|
N

A

A

D

D

A

A

S

A

D

A

L

L

L

L

T

Q

E

Q

F

F

A

A

E

E

I

L

T

T

G

S

V

V

P

P

V

V

I

I

P

P

T
|
T

L
|
L

M
|
M

G

G

W

W

G

G

T

C

I

I

P

P

D

D

D

D

H

H

P

E

L

L

M

M

A

A

G

G

M

M

C
x
V

G
|
G

L

L

Q
|
Q

T
|
T

S

A

H

H

R

R

Y

Y

G

G

N

N

A

A

T

T

L

L

L

L

A

A

S

S

D

D

F

M

V

V

F

F

G

G

I

I

G
|
G

N
|
N

R
|
R

W

F

A

A

N

Q

R
|
R

H
|
H

T

T

G

G

N

S

V

V

P

E

T

K

Y

Y

T

T

E

E

G

G

R

R

K

K

F

I

I

V

H

H

V

I

D
|
D

I
|
I

E

E

P

P

T

T

Q
|
Q

I

I

G

G

R

R

V

V

F

L

A

C

P

P

D

D

F

L

G

G

I

I

V

V

S

S

D
|
D

A
|
A

A

K

A

A

A

A

L

L

K

T

L

L

F

L

L

V

D

E

V

V

A

A

T

Q

E

E

W

M

K

Q

T

K

A

A

G

G

K

R

L

L

R

P

D

C

W

R

S

K

A

E

W

W

A

V

D

A

E

D

C

C

R
x
Q

E

Q

R

R

K

K

R

R

T

T

M

L

L

L

R

R

K

K

T

T

H

H

F

F

D

D

Q

N

T

V

P

P

L

V

K

K

P

P

Q

Q

R

R

V

V

Y

Y

E

E

E

E

M

M

N

N

K

K

A

A

F

F

G

G

R

R

D

D

T

V

C

C

F

Y

V

V

S

T

T

T

I
|
I

G
|
G

L
|
L

S
|
S

Q

Q

I
|
I

A

A

G

A

A

A

Q

Q

F

M

L

L

H

H

V

V

Y

F

R

K

P

D

R

R

N

H

W

W

I

I

N

N

C

C

G
|
G

Q

Q

A

A

G

G

P

P

L
|
L

G

G

W

W

T

T

L

I

P

P

A

A

A

A

L

L

G

G

V

V

R

C

A

A

A

A

D

D

P

P

N

K

R

R

P

N

I

V

V

V

A

A

L

I

S

S

G
|
G

D
|
D

Y
x
F

D
|
D

F

F

Q

Q

F
|
F

L
|
L

I

I

E
|
E

E

E

L

L

A

A

V

V

G

G

A

A

Q

Q

H

F

K

N

L

I

P

P

Y

Y

L

I

H

H

V

V

V

L

V

V

N

N

N
|
N

S

A

Y
|
Y

L
|
L

G
|
G

L
|
L

I
|
I

R

R

Q

Q

A

S

Q

Q

R

M

G

A

F

F

E

D

M

M

D

D

F

Y

E
x
C

V

V

S
x
Q

L

L

A

A

F

F

D

E

N

N

I

I

N

N

A

S

S

S

G

-

D

-

A

E

E

V

K

N

G

G

Y

Y

G

G

V

V

D

D

H

H

V

V

A

K

V

V

A

A

E

E

G

G

L

L

G

G

C

C

K

K

A

A

I

I

R

R

V

V

K

F

S

K

P

P

N

E

E

D

F

I

A

A

D

P

A

A

F

F

E

E

R

Q

A

A

Q

K

A

A

L

L

M

M

E

A

E

Q

H

Y

R

R

V

V

P

P

V

V

V

V

I

V

E

E

F

V

I

I

L

L

E

E

R

R

V

V

T

T

N

N

I

I

A

S

M

M

G

G

A

S

D

E

I

L

N

D

A

N

V

V

V

M

E

E

F

F

E

E

E

D

L

I

A

A

A

D

R

N

G

A

E

A

D

D

A

A

P

P

T

T

binding flavin-adenine dinucleotide: R155 (= R154), P156 (= P155), G212 (= G211), G213 (= G212), G214 (= G213), N217 (= N216), T238 (= T237), L239 (= L238), M240 (= M239), V256 (≠ C255), G257 (= G256), Q259 (= Q258), T260 (= T259), G279 (= G278), N280 (= N279), R281 (= R280), R285 (= R284), H286 (= H285), D303 (= D302), I304 (= I303), Q308 (= Q307), D322 (= D321), A323 (= A322), I394 (= I393), I399 (= I398), G417 (= G416)
binding magnesium ion: D447 (= D446), F452 (= F451), L453 (= L452), E455 (= E454), N474 (= N473), Y476 (= Y475)
binding thiamine diphosphate: I394 (= I393), G395 (= G394), L396 (= L395), S397 (= S396), L422 (= L421), G446 (= G445), D447 (= D446), F448 (≠ Y447), D449 (= D448), N474 (= N473), Y476 (= Y475), L477 (= L476), G478 (= G477), L479 (= L478), I480 (= I479)
binding ubiquinone-1: Q354 (≠ R353), C492 (≠ E491), Q494 (≠ S493)

Sites not aligning to the query:

binding ubiquinone-1: 589

6lpiB Crystal structure of ahas holo-enzyme (see paper)
34% identity, 80% coverage: 11:486/594 of query aligns to 13:468/539 of 6lpiB

query
sites
6lpiB

V

V

H

H

V

F

L

L

E

E

K

Q

E

Q

G

G

V

I

D

K

C

I

A

V

F

T

G

G

V
x
I

P

P

G
|
G

A
x
G

A
x
S

I
|
I

N

L

P

P

L

V

Y

Y

S

D

A

A

L

L

R

S

A

Q

R

S

G

T

S

Q

I

I

R

R

H

H

V

I

L

L

A

A

R

R

H

H

V
x
E

E

Q

G

G

A

A

S

G

H

F

M

I

A

A

E

Q

G

G

Y

M

T

A

R

R

A

T

K

D

H

-

G

G

N

K

I

P

G

A

I

V

C

C

I

M

G

A

T
x
C

S

S

G

G

P
|
P

A

G

G

A

T

T

D

N

M

L

I

V

T

T

G

A

L

I

Y

A

S

D

A

A

S

R

A

L

D

D

S

S

I

I

P

P

I

L

L

I

C

C

I

I

T

T

G

G

Q

Q

A

V

P

P

R

A

A

S

R

M

L

I

D

G

K

T

E

D

D

A

F
|
F

Q
|
Q

A
x
E

V

V

D

D

I

T

A

Y

A

G

I

I

A

S

G

I

P

P

V

I

T

T

K

K

W

H

A

N

V

Y

T

L

V

V

M

R

E

H

P

I

A

E

L

E

V

L

P

P

F

Q

V

V

F

M

Q

S

K

D

A

A

F

F

H

R

L

I

M

A

R

Q

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

W

I

I

D

D

L

I

P

P

V
x
K

D

D

V

V

Q

Q

L

T

A

A

E

V

I

F

E

E

F

I

D

E

P

T

E

Q

T

P

Y

A

E

M

P

A

L

E

A

K

P

A

Y

A

K

A

P

P

A

A

A

F

T

S

R

E

A

E

Q

S

A

I

E

R

K

D

A

A

L

A

A

A

M

M

L

I

N

N

A

A

S

A

E

K

R

R

P

P

L

V

I

L

V

Y

A

L

G
|
G

G
|
G

G
|
G

I

V

I

I

N

N

A

A

D

P

A

A

S

R

D

-

L

-

L

V

T

R

E

E

F

L

A

A

E

E

I

K

T

A

G

Q

V

L

P

P

V

T

I

T

P

M

T
|
T

L
|
L

M
|
M

G

A

W

L

G

G

T

M

I

L

P

P

D

K

D

A

H

H

P

P

L

L

M

S

A

L

G

G

M

M

C
x
L

G

G

L
x
M

Q
x
H

T

G

S

V

H

-

R

R

Y

S

G

T

N

N

A

Y

T

I

L

L

L

Q

A

E

S

A

D

D

F

L

V

L

F

I

G

V

I

L

G
|
G

N
x
A

R
|
R

W

F

A
x
D

N

D

R
|
R

H
x
A

T

I

G

G

N

K

V

T

P

E

T

Q

Y

F

T

C

E

P

G

N

R

A

K

K

F

I

I

I

H

H

V

V

D
|
D

I
|
I

E

D

P

R

T

A

Q

E

I

L

G

G

R

K

V

I

F

K

A

Q

P

P

D

H

F

V

G

A

I

I

V

Q

S

A

D
|
D

A
x
V

A

D

A

D

A

V

L

L

K

A

L

Q

F

L

L

I

D

P

V

L

A

V

T

E

E

A

W

Q

K

P

T

R

A

A

G

-

K

-

L

-

R

-

D

E

W

W

S

H

A

Q

W

L

-

V

A

A

D

D

E

L

C

Q

R

R

E

E

R

-

K

-

R

-

T

-

M

-

L

-

R

-

K

-

T

-

H

-

F

-

D

-

Q

-

T

D

P

P

L

L

K

S

P

H

Q

Y

R

G

V

L

Y

I

E

N

E

A

M

V

N

A

K

A

A

C

F

V

G

D

R

D

D

N

T

A

C

I

F

I

V

T

S

T

T

D

I
x
V

G
|
G

L
x
Q

S
x
H

Q

Q

I
x
M

A

W

G

T

A

A

Q

Q

F

A

L

Y

H

P

V

L

Y

N

R

R

P

P

R

R

N

Q

W

W

I

L

N

T

C

S

G
|
G

Q

G

A

L

G
|
G

P

T

L
x
M

G

G

W

F

T

G

L

L

P

P

A

A

A

A

L

I

G

G

V

A

R

A

A

L

A

A

D

N

P

P

N

D

R

R

P

K

I

V

V

L

A

C

L

F

S

S

G
|
G

D
|
D

Y
x
G

D
x
S

F

L

Q

M

F
x
M

L

N

I

I

E

Q

E

E

L

M

A

A

V

T

G

A

A

S

Q

E

H

N

K

Q

L

L

P

D

Y

V

L

K

H

I

V

I

V

L

V

M

N

N

N
|
N

S

E

Y
x
A

L
|
L

G
|
G

L
|
L

I
x
V

R

H

Q

Q

A
x
Q

Q

Q

R

S

G

L

F

F

active site: I27 (≠ V25), G29 (= G27), G30 (≠ A28), S31 (≠ A29), I32 (= I30), E53 (≠ V51), C76 (≠ T75), F115 (= F114), Q116 (= Q115), E117 (≠ A116), K165 (≠ V164), M256 (≠ L257), A283 (≠ H285), V375 (≠ I393), G401 (= G419), M403 (≠ L421), D428 (= D446), N455 (= N473), A457 (≠ Y475), L458 (= L476), L460 (= L478), V461 (≠ I479), Q464 (≠ A482)
binding flavin-adenine dinucleotide: R155 (= R154), G212 (= G211), G213 (= G212), G214 (= G213), T236 (= T237), L237 (= L238), M238 (= M239), L254 (≠ C255), M256 (≠ L257), H257 (≠ Q258), G276 (= G278), A277 (≠ N279), R278 (= R280), D280 (≠ A282), R282 (= R284), A283 (≠ H285), D300 (= D302), I301 (= I303), D319 (= D321), V320 (≠ A322), M380 (≠ I398), G398 (= G416)
binding magnesium ion: D428 (= D446), N455 (= N473)
binding thiamine diphosphate: E53 (≠ V51), C76 (≠ T75), P79 (= P78), G376 (= G394), Q377 (≠ L395), H378 (≠ S396), G401 (= G419), M403 (≠ L421), G427 (= G445), D428 (= D446), G429 (≠ Y447), S430 (≠ D448), M433 (≠ F451), N455 (= N473), A457 (≠ Y475), L458 (= L476), G459 (= G477), L460 (= L478), V461 (≠ I479)

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
33% identity, 90% coverage: 5:539/594 of query aligns to 95:624/667 of P09342

query
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C161 (= C72) modified: Disulfide link with 307
P194 (= P105) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ I214) modified: Disulfide link with 161

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
33% identity, 81% coverage: 5:486/594 of query aligns to 13:493/582 of 8et4A

query
sites
8et4A

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