SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_015888332.1 NCBI__GCF_000018545.1:WP_015888332.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4k28A 2.15 angstrom resolution crystal structure of a shikimate dehydrogenase family protein from pseudomonas putida kt2440 in complex with NAD+ (see paper)
41% identity, 93% coverage: 9:266/277 of query aligns to 2:262/266 of 4k28A

query
sites
4k28A

G

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binding manganese (ii) ion: C153 (≠ I160), C164 (≠ I171), V176 (≠ A183), F180 (≠ L187)
binding nicotinamide-adenine-dinucleotide: I129 (≠ V136), G130 (= G137), G132 (= G139), G133 (= G140), V134 (≠ A141), C153 (≠ I160), D154 (≠ E161), P155 (≠ R162), R159 (= R166), S193 (= S200), P194 (= P201), V222 (≠ A226), G245 (= G249), M248 (= M252), A249 (≠ T253)

Q9KVT3 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
30% identity, 90% coverage: 16:264/277 of query aligns to 10:256/278 of Q9KVT3

query
sites
Q9KVT3

L

V

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F

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SKS 18:20 (≠ VKS 24:26) binding shikimate
N90 (= N97) binding shikimate
D106 (= D113) binding shikimate
NRTFAK 154:159 (≠ ERDRAR 161:166) binding NADP(+)
Q248 (= Q256) binding shikimate

3pgjA 2.49 angstrom resolution crystal structure of shikimate 5- dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate
30% identity, 90% coverage: 16:264/277 of query aligns to 6:252/272 of 3pgjA

query
sites
3pgjA

L

V

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binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S14 (≠ V24), S16 (= S26), N59 (≠ S70), T61 (= T72), K65 (= K76), N86 (= N97), D102 (= D113), Q244 (= Q256)

1nvtB Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
32% identity, 93% coverage: 7:264/277 of query aligns to 7:267/287 of 1nvtB

query
sites
1nvtB

I

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A

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active site: K75 (= K76), D111 (= D113)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I72 (≠ M73), G135 (= G137), G137 (= G139), G138 (= G140), A139 (= A141), N157 (vs. gap), R158 (vs. gap), T159 (≠ V159), K162 (≠ R162), A200 (= A199), T201 (≠ S200), P202 (= P201), I203 (≠ A202), M205 (= M204), L229 (≠ A226), Y231 (≠ T228), M255 (= M252), L256 (≠ T253)
binding zinc ion: E22 (= E22), H23 (≠ Q23)

1nvtA Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
32% identity, 93% coverage: 7:264/277 of query aligns to 7:267/287 of 1nvtA

query
sites
1nvtA

I

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I

V

I

A

Y

F

A

E

L

L

A

A

E

K

A

D

G

N

A

N

A

I

T

I

I

I

V

A

-
x
N

-
x
R

V
x
T

I

V

E

E

R
x
K

D

A

R

E

A

A

R

L

S

A

D

K

R

E

L

I

I

A

G

E

Q

K

L

L

H

N

R

K

D

K

Y

F

P

G

Q

E

L

E

Q

V

A

K

Y

F

A

S

F

G

L

L

P

D

D

V

D

D

A

L

D

D

-

G

V

V

D

D

I

I

A

I

I

I

N

N

A
|
A

S
x
T

P
|
P

A
x
I

G

G

M
|
M

D

Y

P

P

-

N

A

I

D

D

P

V

H

E

P

P

F

I

-

V

P

K

L

A

E

E

R

K

L

L

T

R

K

E

A

D

E

M

I

V

V

V

A

M

D

D

A
x
L

V

I

T
x
Y

K

N

P

P

P

L

V

E

T

T

P

V

W

L

L

L

E

K

E

E

A

A

R

K

R

V

G

N

I

A

A

K

I

T

Q

I

T

N

G
|
G

A

L

E

G

M
|
M

T
x
L

L

I

A

Y

Q

Q

L

G

P

A

T

V

Q

A

I

F

A

K

F

I

W

W

active site: K75 (= K76), D111 (= D113)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G135 (= G137), A139 (= A141), N157 (vs. gap), R158 (vs. gap), T159 (≠ V159), K162 (≠ R162), A200 (= A199), T201 (≠ S200), P202 (= P201), I203 (≠ A202), M205 (= M204), L229 (≠ A226), Y231 (≠ T228), G252 (= G249), M255 (= M252), L256 (≠ T253)

Q5HNV1 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Staphylococcus epidermidis (strain ATCC 35984 / RP62A) (see paper)
29% identity, 90% coverage: 16:264/277 of query aligns to 5:247/269 of Q5HNV1

query
sites
Q5HNV1

L

V

I

I

G

G

H

N

P

P

V

I

E

S

Q

H

V
x
S

K
x
L

S
|
S

P

P

G

L

P

M

V

H

N

H

A

A

W

N

F

F

S

Q

-

S

-

L

-

N

-

L

D

E

N

N

D

T

V

Y

G

E

A

A

V

I

L

N

I

V

P

P

V

V

D

N

I

Q

F

F

P

Q

E

D

K

-

V

-

P

-

A

-

F

-

L

I

D

K

A

K

I

I

R

I

G

S

T

E

P

K

N

S

C

I

P

D

G

G

V

F

S

N

V

V

T
|
T

M

I

P

P

H

H

K

K

Q

E

A

R

A

I

C

I

A

P

G

Y

V

L

D

D

E

D

L

I

T

N

E

E

R

Q

A

A

R

K

R

S

A

V

E

G

A

A

V

V

N
|
N

I

T

I

V

R

L

R

V

N

-

A

K

D

D

G

G

T

K

L

W

V

I

G

G

D

Y

M

N

V

T

D
|
D

G

G

D

I

A

G

M

Y

V

V

A

N

A

G

L

L

A

K

K

Q

N

I

G

Y

V

E

G

G

V

I

R

E

G

D

K

A

T

Y

V

I

L

L

V

I

V

L

G

G

A

A

G

G

A

A

G

S

M

K

A

G

I

I

I

A

Y

N

A

E

L

L

A

Y

E

K

A

I

G

V

A

R

A

P

T

T

I

L

V

T

V

V

I

A

E

N

R

R

D

T

R

M

A

S

R

R

S

F

D

N

R

N

L

W

-

S

-

L

-

N

I

I

G

N

Q

K

L

I

H

N

R

L

D

S

Y

H

P

A

Q

E

L

S

Q

H

A

L

Y

D

A

E

F

F

L

-

P

-

D

-

A

-

D

-

V

-

D

D

I

I

A

I

I

I

N

N

A

T

S

T

P

P

A

A

G

G

M

M

D

N

P

G

A

N

D

T

P

D

H

S

P

V

F

I

P

S

L

L

E

N

R

R

L

L

T

A

K

S

A

H

E

T

I

L

V

V

A

S

D

D

A

I

V

V

T
x
Y

K

N

P

P

P

Y

V

K

T

T

P

P

W

I

L

L

E

I

E

E

A

A

R

E

R

Q

R

R

G

G

I

N

A

P

I

I

Q

Y

T

N

G

G

A

L

E

D

M

M

T

F

L

V

A

H

Q
|
Q

L

G

P

A

T

E

Q

S

I

F

A

K

F

I

W

W

SLS 13:15 (≠ VKS 24:26) binding shikimate
T60 (= T72) binding shikimate
N85 (= N97) binding shikimate
D100 (= D113) binding shikimate
Y211 (≠ T228) Plays a major role in the catalytic process and a minor role in the substrate binding; mutation to F: Leads to a 345-fold decrease in the catalytic efficiency and a 3-fold decrease in the affinity binding for shikimate.
Q239 (= Q256) binding shikimate

Q58484 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
32% identity, 93% coverage: 7:264/277 of query aligns to 2:262/282 of Q58484

query
sites
Q58484

I

I

T

N

G

A

K

K

T

T

V

K

F

V

I

I

P

G

L

L

I

I

G

G

H

H

P

P

V

V

E

E

Q

H

V

S

K

F

S

S

P

P

G

I

P

M

V

H

N

N

A

A

W

A

F

F

S

K

D

D

N

K

D

G

V

L

G

N

A

Y

V

V

L

Y

I

V

P

A

V

F

D

D

I

V

F

L

P

P

E

E

K

N

V

L

P

K

A

Y

F

V

L

I

D

D

A

G

I

A

R

K

G

A

T

L

P

-

N

G

C

I

P

V

G

G

V

F

S

N

V

V

T

T

M

I

P

P

H

H

K

K

Q

I

A

E

A

I

C

M

A

K

G

Y

V

L

D

D

E

E

L

I

T

D

E

K

R

D

A

A

R

Q

R

L

A

I

E

G

A

A

V

V

N

N

I

T

I

I

R

K

R

I

N

-

A

E

D

D

G

G

T

K

L

A

V

I

G

G

D

Y

M

N

V

T

D

D

G

G

D

I

A

G

M

A

V

R

A

M

A

A

L

L

A

E

K

E

N

E

G

I

V

G

G

R

V

V

R

K

G

D

K

K

T

N

V

I

L

V

V

I

V

Y

G
|
G

A
|
A

G
|
G

A
|
A

G

A

M

R

A

A

I

V

I

A

Y

F

A

E

L

L

A

A

E

K

A

D

G

N

A

N

A

I

T

I

I

I

V

A

-
x
N

-
x
R

V
x
T

I
x
V

E
|
E

R
x
K

D

A

R

E

A

A

R

L

S

A

D

K

R

E

L

I

I

A

G

E

Q

K

L

L

H

N

R

K

D

K

Y

F

P

G

Q

E

L

E

Q

V

A

K

Y

F

A

S

F

G

L

L

P

D

D

V

D

D

A

L

D

D

-

G

V

V

D

D

I

I

A

I

I

I

N

N

A

A

S
x
T

P

P

A

I

G

G

M
|
M

D

Y

P

P

-

N

A

I

D

D

P

V

H

E

P

P

F

I

-

V

P

K

L

A

E

E

R

K

L

L

T

R

K

E

A

D

E

M

I

V

V

V

A

M

D

D

A
x
L

V

I

T

Y

K

N

P

P

P

L

V

E

T

T

P

V

W

L

L

L

E

K

E

E

A

A

R

K

R

V

G

N

I

A

A

K

I

T

Q

I

T

N

G

G

A

L

E

G

M

M

T

L

L

I

A

Y

Q

Q

L

G

P

A

T

V

Q

A

I

F

A

K

F

I

W

W

GAGGA 130:134 (= GAGGA 137:141) binding NADP(+)
NRTVEK 152:157 (≠ --VIER 159:162) binding NADP(+)
T196 (≠ S200) binding NADP(+)
M200 (= M204) binding NADP(+)
L224 (≠ A226) binding NADP(+)

1npdB X-ray structure of shikimate dehydrogenase complexed with NAD+ from e.Coli (ydib) northeast structural genomics research consortium (nesg) target er24 (see paper)
28% identity, 94% coverage: 6:264/277 of query aligns to 2:270/288 of 1npdB

query
sites
1npdB

E

D

I

V

T

T

G

A

K

K

T

Y

V

E

F

L

I

I

P

G

L

L

I

M

G

A

H

Y

P

P

V

I

E

R

Q

H

V

S

K

L

S

S

P

P

G

E

P

M

V

Q

N

N

A

K

W

A

F

L

S

E

D

K

N

A

D

G

V

L

G

P

A

F

V

T

L

Y

I

M

P

A

V

F

D

E

I

V

F

D

P

N

E

D

K

S

V

F

P

P

A

G

F

A

L

I

D

E

A

G

I

L

R

K

G

A

T

L

P

K

N

-

C

M

P

R

G

G

V

T

S

G

V

V

T

S

M

M

P

P

H

N

K

K

Q

Q

A

L

A

A

C

C

A

E

G

Y

V

V

D

D

E

E

L

L

T

T

E

P

R

A

A

A

R

K

R

L

A

V

E

G

A

A

V

I

N

N

I

T

I

I

R

V

R

-

N

N

A

D

D

D

G

G

T

Y

L

L

V

R

G

G

D

Y

M

N

V

T

D

D

G

G

D

T

A

G

M

H

V

I

A

R

A

A

L

I

A

K

K

E

N

S

G

G

V

F

G

D

V

I

R

K

G

G

K

K

T

T

V

M

L

V

V

L

G

G

A
|
A

G
|
G

A
|
A

G

S

M

T

A

A

I

I

I

G

Y

A

A

Q

L

G

A

A

E

I

A

E

G

G

A

L

A

K

T

E

I

I

V

K

V

L

I

F

E
x
N

R
|
R

D

R

R
x
D

A

E

R
x
F

S

F

D

D

R

K

L

A

I

L

G

A

Q

F

L

A

H

Q

R

R

-

V

-

N

-

E

-

N

-

T

-

D

-

C

-

V

-

T

-

V

-

T

D

D

Y

L

P

A

Q

D

L

Q

Q

Q

A

A

Y

F

A

A

F

-

L

-

P

E

D

A

D

L

A

A

D

S

V

A

D

D

I

I

A

L

I

T

N

N

A

G

S
x
T

P
x
K

A
x
V

G

G

M
|
M

D

K

P

P

A

L

D

E

P

N

H

E

P

S

F

L

-

V

-

N

P

D

L

I

E

S

R

L

L

L

T

H

K

P

A

G

E

L

I

L

V

V

A

T

D

E

A
x
C

V

V

T

Y

K

N

P

P

P

H

V

M

T

T

P

K

W

L

L

L

E

Q

A

A

R

Q

R

A

G

G

I

C

A

K

I

T

Q

I

T

D

G

G

A

Y

E

G

M
|
M

T
x
L

L

L

A

W

Q

Q

L

G

P

A

T

E

Q

Q

I

F

A

T

F

L

W

W

binding nicotinamide-adenine-dinucleotide: A132 (= A138), G133 (= G139), G134 (= G140), A135 (= A141), N155 (≠ E161), R156 (= R162), D158 (≠ R164), F160 (≠ R166), T204 (≠ S200), K205 (≠ P201), V206 (≠ A202), M208 (= M204), C232 (≠ A226), M258 (= M252), L259 (≠ T253)

P0A6D5 Quinate/shikimate dehydrogenase; NAD-dependent shikimate 5-dehydrogenase; EC 1.1.1.282 from Escherichia coli (strain K12) (see 4 papers)
28% identity, 94% coverage: 6:264/277 of query aligns to 2:270/288 of P0A6D5

query
sites
P0A6D5

E

D

I

V

T

T

G

A

K

K

T

Y

V

E

F

L

I

I

P

G

L

L

I

M

G

A

H

Y

P

P

V

I

E

R

Q

H

V

S

K

L

S
|
S

P

P

G

E

P

M

V

Q

N

N

A

K

W

A

F

L

S

E

D

K

N

A

D

G

V

L

G

P

A

F

V

T

L
x
Y

I

M

P

A

V

F

D

E

I

V

F

D

P

N

E

D

K

S

V

F

P

P

A

G

F

A

L

I

D

E

A

G

I

L

R

K

G

A

T

L

P

K

N

-

C

M

P

R

G

G

V

T

S

G

V

V

T
x
S

M

M

P

P

H

N

K
|
K

Q

Q

A

L

A

A

C

C

A

E

G

Y

V

V

D

D

E

E

L

L

T

T

E

P

R

A

A

A

R

K

R

L

A

V

E

G

A

A

V

I

N
|
N

I

T

I

I

R

V

R

-

N

N

A

D

D

D

G

G

T

Y

L

L

V

R

G

G

D

Y

M

N

V
x
T

D
|
D

G

G

D

T

A

G

M

H

V

I

A

R

A

A

L

I

A

K

K

E

N

S

G

G

V

F

G

D

V

I

R

K

G

G

K

K

T

T

V

M

L

V

V

L

G

G

A
|
A

G
|
G

A
|
A

G

S

M

T

A

A

I

I

I

G

Y

A

A

Q

L

G

A

A

E

I

A

E

G

G

A

L

A

K

T

E

I

I

V

K

V

L

I

F

E
x
N

R
|
R

D
x
R

R
x
D

A

E

R

F

S

F

D

D

R

K

L

A

I

L

G

A

Q

F

L

A

H

Q

R

R

-

V

-

N

-

E

-

N

-

T

-

D

-

C

-

V

-

T

-

V

-

T

D

D

Y

L

P

A

Q

D

L

Q

Q

Q

A

A

Y

F

A

A

F

-

L

-

P

E

D

A

D

L

A

A

D

S

V

A

D

D

I

I

A

L

I

T

N

N

A

G

S

T

P
x
K

A

V

G

G

M

M

D

K

P

P

A

L

D

E

P

N

H

E

P

S

F

L

-

V

-

N

P

D

L

I

E

S

R

L

L

L

T

H

K

P

A

G

E

L

I

L

V

V

A

T

D

E

A
x
C

V
|
V

T
x
Y

K
x
N

P

P

P

H

V

M

T

T

P

K

W

L

L

L

E

Q

A

A

R

Q

R

A

G

G

I

C

A

K

I

T

Q

I

T

D

G
|
G

A

Y

E

G

M

M

T

L

L

L

A

W

Q
|
Q

L

G

P

A

T

E

Q

Q

I

F

A

T

F

L

W

W

S22 (= S26) mutation to A: Kinetically unchanged as compared with the wild-type.
Y39 (≠ L43) mutation to F: Kinetically unchanged as compared with the wild-type.
S67 (≠ T72) mutation to A: Reduces activity towards quinate about 6-fold, but has a little effect on shikimate conversion.
K71 (= K76) mutation to A: 3200-fold decrease in the affinity for quinate. 170-fold decrease in the affinity for shikimate.; mutation to G: 10-fold greater reduction in catalytic efficiency is observed with quinate than with shikimate.
N92 (= N97) mutation to A: Alters protein structure. Loss of activity for both substrates.
T106 (≠ V112) mutation to A: 2000-fold decrease in the affinity for quinate. 70-fold decrease in the affinity for shikimate. 10-fold greater reduction in catalytic efficiency is observed with quinate than with shikimate.
D107 (= D113) mutation to A: Loss of activity towards quinate. 20000-fold decrease in the affinity for shikimate.
AGGA 132:135 (= AGGA 138:141) binding NAD(+)
NRRD 155:158 (≠ ERDR 161:164) binding NAD(+)
K205 (≠ P201) binding NAD(+)
CVYN 232:235 (≠ AVTK 226:229) binding NAD(+)
G255 (= G249) binding NAD(+)
Q262 (= Q256) mutation to A: 3-fold reduction in catalytic efficiency for both substrates.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3sefA 2.4 angstrom resolution crystal structure of shikimate 5-dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate and NADPH
32% identity, 71% coverage: 68:264/277 of query aligns to 53:248/268 of 3sefA

query
sites
3sefA

G

G

V

C

S

N

V

V

T

T

M

V

P

P

H

F

K

K

Q

E

A

E

A

A

C

Y

A

R

G

F

V

A

D

D

E

R

L

L

T

T

E

E

R

R

A

A

R

R

R

L

A

A

E

G

A

A

V

V

N

N

I

T

I

L

R

K

N

L

A

D

D

D

G

G

T

E

L

I

V

L

G

G

D

D

M

N

V

T

D

D

G

G

D

E

A

G

M

L

V

V

A

Q

A

D

L

L

A

L

K

A

N

Q

G

Q

V

V

G

L

V

L

R

K

G

G

K

A

T

T

V

I

L

L

V

L

V

I

G

G

A

A

G
|
G

G

G

A
|
A

G

A

M

R

A

G

I

V

I

L

Y

K

A

P

L

L

A

L

E

D

A

Q

G

Q

A

P

A

A

T

S

I

I

V

T

V

V

I

T

E
x
N

R
|
R

D
x
T

R

F

A

A

R

K

S

A

D

E

R

Q

L

L

I

-

G

A

Q

E

L

L

H

V

R

A

D

A

Y

Y

P

G

Q

E

L

V

Q

K

A

A

Y

Q

A

A

F

F

L

E

P

Q

D

L

D

K

A

Q

D

S

V

Y

D

D

I

V

A

I

I

I

N

N

A
x
S

S
x
T

P
x
S

A
|
A

G

S

M

L

D

D

P

G

A

E

D

L

P

P

H

A

P

I

F

D

P

P

L

V

E

-

R

I

L

F

T

S

K

S

A

R

E

S

I

V

V

C

A

Y

D

D

A

M

V
x
M

T

Y

K

G

P

K

P

G

V

Y

T

T

P

V

W

F

L

N

E

Q

E

W

A

A

R

R

R

Q

R

H

G

G

I

C

A

A

I

Q

Q

A

T

I

-

D

G

G

A

L

E

G

M

M

T

L

L

V

A

G

Q

Q

L

A

P

A

T

E

Q

S

I

F

A

M

F

L

W

W

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G124 (= G139), A126 (= A141), N146 (≠ E161), R147 (= R162), T148 (≠ D163), S183 (≠ A199), T184 (≠ S200), S185 (≠ P201), A186 (= A202), M210 (≠ V227)

3sefC 2.4 angstrom resolution crystal structure of shikimate 5-dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate and NADPH
33% identity, 69% coverage: 16:206/277 of query aligns to 6:192/244 of 3sefC

query
sites
3sefC

L

V

I

F

G

G

H

N

P

P

V

I

E

N

Q

H

V
x
S

K

K

S
|
S

P

P

G

-

P

F

V

I

N

H

A

T

W

L

F

F

S

A

D

R

N

Q

D

T

V

Q

G

Q

A

S

V

M

L

I

-

Y

-

T

-

A

-

Q

-

C

I

V

P

P

V

V

D

D

I

G

F

F

P

T

E

E

K

A

V

A

P

K

A

H

F

F

L

F

D

-

A

-

I

A

R

Q

G

G

T

G

P

R

N

G

C

C

P

-

G

-

V

-

S

N

V

V

T

T

M

V

P

P

H

F

K
|
K

Q

E

A

E

A

A

C

Y

A

R

G

F

V

A

D

D

E

R

L

L

T

T

E

E

R

R

A

A

R

R

R

L

A

A

E

G

A

A

V

V

N

N

I

T

I

L

R

K

N

D

A

-

D

D

G

G

T

E

L

I

V

L

G

G

D

D

M

N

V

T

D
|
D

G

G

D

E

A

G

M

L

V

V

A

Q

A

D

L

L

A

L

K

A

N

Q

G

Q

V

V

G

L

V

L

R

K

G

G

K

A

T

T

V

I

L

L

V

L

V

I

G

G

A

A

G

G

A

A

G

A

M

R

A

G

I

V

I

L

Y

K

A

P

L

L

A

L

E

D

A

Q

G

Q

A

P

A

A

T

S

I

I

V

T

V

V

I

T

E

N

R

R

D

T

R

F

A

A

R

K

S

A

D

E

R

Q

L

L

I

-

G

A

Q

E

L

L

H

V

R

A

D

A

Y

Y

P

G

Q

E

L

V

Q

K

A

A

Y

Q

A

A

F

F

L

E

P

Q

D

L

D

K

A

Q

D

S

V

Y

D

D

I

V

A

I

I

I

N

N

A

S

S

T

P

S

A

A

G

-

M

I

D

D

P

P

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S14 (≠ V24), S16 (= S26), K65 (= K76), D101 (= D113)

Sites not aligning to the query:

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 217

1o9bA Quinate/shikimate dehydrogenase ydib complexed with nadh (see paper)
28% identity, 91% coverage: 13:264/277 of query aligns to 3:264/280 of 1o9bA

query
sites
1o9bA

F

L

I

I

P

G

L

L

I

M

G

A

H

Y

P

P

V

I

E

R

Q

H

V

S

K

L

S

S

P

P

G

E

P

M

V

Q

N

N

A

K

W

A

F

L

S

E

D

K

N

A

D

G

V

L

G

P

A

F

V

T

L

Y

I

M

P

A

V

F

D

E

I

V

F

D

P

N

E

D

K

S

V

F

P

P

A

G

F

A

L

I

D

E

A

G

I

L

R

K

G

A

T

L

P

K

N

-

C

M

P

R

G

G

V

T

S

G

V

V

T

S

M

M

P

P

H

N

K

K

Q

Q

A

L

A

A

C

C

A

E

G

Y

V

V

D

D

E

E

L

L

T

T

E

P

R

A

A

A

R

K

R

L

A

V

E

G

A

A

V

I

N

N

I

T

I

I

R

V

R

-

N

N

A

D

D

D

G

G

T

Y

L

L

V

R

G

G

D

Y

M

N

V

T

D

D

G

G

D

T

A

G

M

H

V

I

A

R

A

A

L

I

A

K

K

E

N

S

G

G

V

F

G

D

V

I

R

K

G

G

K

K

T

T

V

M

L

V

V

L

G

G

A
|
A

G
|
G

A
|
A

G

S

M

T

A

A

I

I

I

G

Y

A

A

Q

L

G

A

A

E

I

A

E

G

G

A

L

A

K

T

E

I

I

V

K

V

L

I

F

E

N

R
|
R

D

R

R

D

A

E

R
x
F

S

F

D

D

R

K

L

A

I

L

G

A

Q

F

L

A

H

Q

R

R

-

V

-

N

-

E

-

N

-

T

-

D

-

C

-

V

-

T

-

V

-

T

D

D

Y

L

P

A

Q

D

L

Q

Q

Q

A

A

Y

F

A

A

F

-

L

-

P

E

D

A

D

L

A

A

D

S

V

A

D

D

I

I

A

L

I

T

N

N

A

G

S

T

P
x
K

A
x
V

G

G

M
|
M

D

K

P

P

A

L

D

E

P

N

H

E

P

S

F

L

-

V

-

N

P

D

L

I

E

S

R

L

L

L

T

H

K

P

A

G

E

L

I

L

V

V

A

T

D

E

A
x
C

V

V

T
x
Y

K

N

P

P

P

H

V

M

T

T

P

K

W

L

L

L

E

Q

A

A

R

Q

R

A

G

G

I

C

A

K

I

T

Q

I

T

D

G

G

A

Y

E

G

M
|
M

T
x
L

L

L

A

W

Q

Q

L

G

P

A

T

E

Q

Q

I

F

A

T

F

L

W

W

binding 1,4-dihydronicotinamide adenine dinucleotide: A126 (= A138), G127 (= G139), G128 (= G140), A129 (= A141), R150 (= R162), F154 (≠ R166), K199 (≠ P201), V200 (≠ A202), M202 (= M204), C226 (≠ A226), Y228 (≠ T228), M252 (= M252), L253 (≠ T253)

3dooA Crystal structure of shikimate dehydrogenase from staphylococcus epidermidis complexed with shikimate (see paper)
28% identity, 90% coverage: 16:264/277 of query aligns to 5:238/258 of 3dooA

query
sites
3dooA

L

V

I

I

G

G

H

N

P

P

V

I

E

S

Q

H

V
x
S

K

L

S
|
S

P

P

G

L

P

M

V

H

N

H

A

A

W

N

F

F

S

Q

-

S

-

L

-

N

-

L

D

E

N

N

D

T

V

Y

G

E

A

A

V

I

L

N

I

V

P

P

V

V

D

N

I

Q

F

F

P

Q

E

D

K

-

V

-

P

-

A

-

F

-

L

I

D

K

A

K

I

I

R

I

G

S

T

E

P

K

N

S

C

I

P

D

G

G

V

F

S
x
N

V

V

T
|
T

M

I

P

P

H

H

K
|
K

Q

E

A

R

A

I

C

I

A

P

G

Y

V

L

D

D

E

D

L

I

T

N

E

E

R

Q

A

A

R

K

R

S

A

V

E

G

A

A

V

V

N
|
N

I

T

I

V

R

L

R

V

N

-

A

K

D

D

G

G

T

K

L

W

V

I

G

G

D

Y

M

N

V

T

D
|
D

G

G

D

I

A

G

M

Y

V

V

A

N

A

G

L

L

A

K

K

Q

N

I

G

Y

V

E

G

G

V

I

R

E

G

D

K

A

T

Y

V

I

L

L

V

I

V

L

G

G

A

A

G

G

A

A

G

S

M

K

A

G

I

I

I

A

Y

N

A

E

L

L

A

Y

E

K

A

I

G

V

A

R

A

P

T

T

I

L

V

T

V

V

I

A

E

N

R

R

D

T

R

M

A

S

R

R

S

F

D

N

R

N

L

W

-

S

-

L

-

N

I

I

G

N

Q

K

L

I

H

N

R

L

D

S

Y

H

P

A

Q

E

L

S

Q

H

A

L

Y

D

A

E

F

F

L

-

P

-

D

-

A

-

D

-

V

-

D

D

I

I

A

I

I

I

N

N

A

T

S

T

P

P

A

V

G

-

M

-

D

-

P

-

A

-

D

-

P

-

H

-

P

-

F

I

P

S

L

L

E

N

R

R

L

L

T

A

K

S

A

H

E

T

I

L

V

V

A

S

D

D

A

I

V

V

T

Y

K

N

P

P

P

Y

V

K

T

T

P

P

W

I

L

L

E

I

E

E

A

A

R

E

R

Q

R

R

G

G

I

N

A

P

I

I

Q

Y

T

N

G

G

A

L

E

D

M

M

T
x
F

L

V

A

H

Q
|
Q

L

G

P

A

T

E

Q

S

I

F

A

K

F

I

W

W

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S13 (≠ V24), S15 (= S26), N58 (≠ S70), T60 (= T72), K64 (= K76), N85 (= N97), D100 (= D113), F227 (≠ T253), Q230 (= Q256)

2hk9A Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
26% identity, 94% coverage: 7:265/277 of query aligns to 2:251/269 of 2hk9A

query
sites
2hk9A

I

I

T

N

G

A

K

Q

T

T

V

Q

F

L

I

Y

P

G

L

V

I

I

G

G

H

F

P

P

V

V

E

K

Q

H

V
x
S

K

L

S
|
S

P

P

G

V

P

F

V

Q

N

N

A

A

W

L

F

I

S

R

D

Y

N

A

D

G

V

L

G

N

A

A

V

V

L

Y

I

L

P

A

V

F

D

E

I

I

F

N

P

P

E

E

K

E

V

L

P

K

A

K

F

A

L

F

D

E

A

G

I

F

R

K

G

A

T

L

P

-

N

K

C

V

P

K

G

G

V

I

S
x
N

V

V

T

T

M
x
V

P

P

H

F

K
|
K

Q

E

A

E

A

I

C

I

A

P

G

L

V

L

D

D

E

Y

L

V

T

E

E

D

R

T

A

A

R

K

R

E

A

I

E

G

A

A

V

V

N
|
N

I

T

I

V

R

K

R

F

N

E

A

-

D

N

G

G

T

K

L

A

V

Y

G

G

D

Y

M

N

V

T

D
|
D

G

W

D

I

A

G

M

F

V

L

A

K

A

S

L

L

A

K

K

S

N

L

G

I

V

P

G

E

V

V

R

K

G

E

K

K

T

S

V

I

L

L

V

V

V

L

G

G

A

A

G
|
G

A
|
A

G

S

M

R

A

A

I

V

I

I

Y

Y

A

A

L

L

A

V

E

K

A

E

G

G

A

A

A

K

T

V

I

F

V

L

V

-

I

-

E

-

R

W

D
x
N

R
|
R

A
x
T

R

K

S

E

D

K

R

A

L

I

I

-

G

-

Q

K

L

L

H

A

R

Q

D

K

Y

F

P

P

Q

L

L

E

Q

V

A

V

Y

N

A

S

F

-

L

-

P

P

D

E

A

V

-

I

-

D

D

K

V

V

D

Q

I

V

A

I

I

V

N

N

A

T

S
x
T

P
x
S

A
x
V

G

G

M

L

D

K

P

D

A

E

D

D

P

P

H

E

P

I

F

F

P

N

L

Y

E

D

R

L

L

I

T

K

K

K

A

D

E

H

I

V

V

V

A

V

D

D

A

I

V

I

T
x
Y

K

K

P

E

P

-

V

-

T

T

P

K

W

L

L

L

E

K

A

A

R

K

R

E

R

K

G

G

I

A

A

K

I

L

Q

L

T

D

G

G

A

L

E

P

M

M

T
x
L

L

L

A

W

Q
|
Q

L

G

P

I

T

E

Q

A

I

F

A

K

F

I

W

W

G

N

binding 2'-monophosphoadenosine-5'-diphosphate: V67 (≠ M73), G132 (= G139), G133 (= G140), A134 (= A141), N153 (≠ D163), R154 (= R164), T155 (≠ A165), T188 (≠ S200), S189 (≠ P201), V190 (≠ A202)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (≠ V24), S21 (= S26), N64 (≠ S70), K70 (= K76), N91 (= N97), D106 (= D113), Y216 (≠ T228), L239 (≠ T253), Q242 (= Q256)

2hk9B Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
26% identity, 94% coverage: 7:265/277 of query aligns to 2:251/267 of 2hk9B

query
sites
2hk9B

I

I

T

N

G

A

K

Q

T

T

V

Q

F

L

I

Y

P

G

L

V

I

I

G

G

H

F

P

P

V

V

E

K

Q

H

V
x
S

K

L

S
|
S

P

P

G

V

P

F

V

Q

N

N

A

A

W

L

F

I

S

R

D

Y

N

A

D

G

V

L

G

N

A

A

V

V

L

Y

I

L

P

A

V

F

D

E

I

I

F

N

P

P

E

E

K

E

V

L

P

K

A

K

F

A

L

F

D

E

A

G

I

F

R

K

G

A

T

L

P

-

N

K

C

V

P

K

G

G

V

I

S
x
N

V

V

T
|
T

M
x
V

P

P

H

F

K
|
K

Q

E

A

E

A

I

C

I

A

P

G

L

V

L

D

D

E

Y

L

V

T

E

E

D

R

T

A

A

R

K

R

E

A

I

E

G

A

A

V

V

N
|
N

I

T

I

V

R

K

R

F

N

E

A

-

D

N

G

G

T

K

L

A

V

Y

G

G

D

Y

M

N

V

T

D
|
D

G

W

D

I

A

G

M

F

V

L

A

K

A

S

L

L

A

K

K

S

N

L

G

I

V

P

G

E

V

V

R

K

G

E

K

K

T

S

V

I

L

L

V

V

V

L

G
|
G

A

A

G

G

G
|
G

A
|
A

G

S

M

R

A

A

I

V

I

I

Y

Y

A

A

L

L

A

V

E

K

A

E

G

G

A

A

A

K

T

V

I

F

V

L

V

-

I

-

E

-

R

W

D
x
N

R
|
R

A
x
T

R

K

S

E

D
x
K

R

A

L

I

I

-

G

-

Q

K

L

L

H

A

R

Q

D

K

Y

F

P

P

Q

L

L

E

Q

V

A

V

Y

N

A

S

F

-

L

-

P

P

D

E

A

V

-

I

-

D

D

K

V

V

D

Q

I

V

A

I

I

V

N

N

A

T

S
x
T

P
x
S

A
x
V

G

G

M

L

D

K

P

D

A

E

D

D

P

P

H

E

P

I

F

F

P

N

L

Y

E

D

R

L

L

I

T

K

K

K

A

D

E

H

I

V

V

V

A

V

D

D

A
x
I

V

I

T
x
Y

K

K

P

E

P

-

V

-

T

T

P

K

W

L

L

L

E

K

A

A

R

K

R

E

R

K

G

G

I

A

A

K

I

L

Q

L

T

D

G

G

A

L

E

P

M
|
M

T
x
L

L

L

A

W

Q
|
Q

L

G

P

I

T

E

Q

A

I

F

A

K

F

I

W

W

G

N

binding nadp nicotinamide-adenine-dinucleotide phosphate: V67 (≠ M73), G130 (= G137), G133 (= G140), A134 (= A141), N153 (≠ D163), R154 (= R164), T155 (≠ A165), K158 (≠ D168), T188 (≠ S200), S189 (≠ P201), V190 (≠ A202), I214 (≠ A226), M238 (= M252), L239 (≠ T253)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (≠ V24), S21 (= S26), N64 (≠ S70), T66 (= T72), K70 (= K76), N91 (= N97), D106 (= D113), Y216 (≠ T228), L239 (≠ T253), Q242 (= Q256)

O67049 Shikimate dehydrogenase (NADP(+)); SD; SDH; EC 1.1.1.25 from Aquifex aeolicus (strain VF5) (see paper)
26% identity, 94% coverage: 7:265/277 of query aligns to 2:251/269 of O67049

query
sites
O67049

I

I

T

N

G

A

K

Q

T

T

V

Q

F

L

I

Y

P

G

L

V

I

I

G

G

H

F

P

P

V

V

E

K

Q

H

V
x
S

K
x
L

S
|
S

P

P

G

V

P

F

V

Q

N

N

A

A

W

L

F

I

S

R

D

Y

N

A

D

G

V

L

G

N

A

A

V

V

L

Y

I

L

P

A

V

F

D

E

I

I

F

N

P

P

E

E

K

E

V

L

P

K

A

K

F

A

L

F

D

E

A

G

I

F

R

K

G

A

T

L

P

-

N

K

C

V

P

K

G

G

V

I

S

N

V

V

T

T

M

V

P

P

H

F

K

K

Q

E

A

E

A

I

C

I

A

P

G

L

V

L

D

D

E

Y

L

V

T

E

E
x
D

R

T

A

A

R

K

R

E

A

I

E

G

A

A

V

V

N
|
N

I

T

I

V

R

K

R

F

N

E

A

-

D

N

G

G

T

K

L

A

V

Y

G

G

D

Y

M

N

V

T

D
|
D

G

W

D

I

A

G

M

F

V

L

A

K

A

S

L

L

A

K

K

S

N

L

G

I

V

P

G

E

V

V

R

K

G

E

K

K

T

S

V

I

L

L

V

V

V

L

G
|
G

A
|
A

G
|
G

A
|
A

G

S

M

R

A

A

I

V

I

I

Y

Y

A

A

L

L

A

V

E

K

A

E

G

G

A

A

A

K

T

V

I

F

V

L

V

-

I

-

E

-

R

W

D

N

R

R

A

T

R

K

S

E

D

K

R

A

L

I

I

-

G

-

Q

K

L

L

H

A

R

Q

D

K

Y

F

P

P

Q

L

L

E

Q

V

A

V

Y

N

A

S

F

-

L

-

P

P

D

E

A

V

-

I

-

D

D

K

V

V

D

Q

I

V

A

I

I

V

N

N

A

T

S

T

P

S

A

V

G

G

M

L

D

K

P

D

A

K

D

D

P

P

H

E

P

I

F

F

P

N

L

Y

E

D

R

L

L

I

T

K

K

K

A

D

E

H

I

V

V

V

A

V

D

D

A
x
I

V

I

T
x
Y

K

K

P

E

P

-

V

-

T

T

P

K

W

L

L

L

E

K

A

A

R

K

R

E

R

K

G

G

I

A

A

K

I

L

Q

F

T

D

G
|
G

A

L

E

P

M

M

T

L

L

L

A

W

Q
|
Q

L

G

P

I

T

E

Q

A

I

F

A

K

F

I

W

W

G

N

SLS 19:21 (≠ VKS 24:26) binding shikimate
D82 (≠ E88) binding NADP(+)
N91 (= N97) binding shikimate
D106 (= D113) binding shikimate
GAGGA 130:134 (= GAGGA 137:141) binding NADP(+)
I214 (≠ A226) binding NADP(+)
Y216 (≠ T228) binding shikimate
G235 (= G249) binding NADP(+)
Q242 (= Q256) binding shikimate

3tnlA 1.45 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with shikimate and NAD.
28% identity, 93% coverage: 7:264/277 of query aligns to 6:273/288 of 3tnlA

query
sites
3tnlA

I

I

T

T

G

G

K

H

T

T

V

E

F

L

I

I

P

G

L

L

I

I

G

A

H

T

P

P

V

I

E

R

Q

H

V
x
S

K

L

S
|
S

P

P

G

T

P

M

V

H

N

N

A

E

W

A

F

F

S

A

D

K

N

L

D

G

V

L

G

D

A

Y

V

V

L

Y

I

L

P

A

V

F

D

E

I

V

F

G

P

D

E

K

K

E

V

L

P

K

A

D

F

V

L

V

D

Q

A

G

I

F

R

R

G

A

T

M

P

-

N

N

C

L

P

R

G

G

V

W

S
x
N

V

V

T
x
S

M
|
M

P

P

H

N

K
|
K

Q

T

A

N

A

I

C

H

A

K

G

Y

V

L

D

D

E

K

L

L

T

S

E

P

R

A

A

A

R

E

R

L

A

V

E

G

A

A

V

V

N
|
N

I

T

I

V

R

V

R

-

N

N

A

D

D

D

G

G

T

V

L

L

V

T

G

G

D

H

M

I

V

T

D
|
D

G

G

D

T

A

G

M

Y

V

M

A

R

A

A

L

L

A

K

K

E

N

A

G

G

V

H

G

D

V

I

R

I

G

G

K

K

T

K

V

M

L

T

V

I

V

C

G
|
G

A
|
A

G
|
G

A
|
A

G

A

M

T

A

A

I

I

I

C

Y

I

A

Q

L

A

A

A

E

L

A

D

G

G

A

V

A

K

T

E

I

I

V

S

V

I

I

F

E
x
N

R
|
R

D

K

-
x
D

-

D

-
x
F

R

Y

A

A

R

N

S

A

D

E

R

K

L

T

I

V

G

E

Q

K

L

I

H

N

-

S

-

K

-

T

-

D

-

C

-

K

-

A

-

Q

-

L

-

F

-

D

-

I

R

E

D

D

Y

H

P

E

Q

Q

L

L

Q

R

A

-

Y

-

A

-

F

-

L

-

P

K

D

E

D

I

A

A

D

E

V

S

D

V

I

I

A

F

I

T

N

N

A

A

S
x
T

P

G

A
x
V

G

G

M
|
M

D

K

P

P

A
x
F

D

E

P

G

H

E

P

T

-

L

F

L

P

P

L

S

E

A

R

D

L

M

T

L

K

R

A

P

E

E

-

L

I

I

V

V

A

S

D

D

A
x
V

V

V

T
x
Y

K

K

P

P

P

T

V

K

T

T

P

R

W

L

L

L

E

E

E

I

A

A

R

E

R

Q

G

G

I

C

A

Q

I

T

Q

L

T

N

G

G

A

L

E

G

M
|
M

T
x
M

L

L

A

W

Q
|
Q

L

G

P

A

T

K

Q

A

I

F

A

E

F

I

W

W

binding nicotinamide-adenine-dinucleotide: M71 (= M73), G134 (= G137), A135 (= A138), G136 (= G139), G137 (= G140), A138 (= A141), N158 (≠ E161), R159 (= R162), D161 (vs. gap), F163 (vs. gap), T207 (≠ S200), V209 (≠ A202), M211 (= M204), F214 (≠ A207), V235 (≠ A226), Y237 (≠ T228), M261 (= M252), M262 (≠ T253)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S23 (≠ V24), S25 (= S26), N68 (≠ S70), S70 (≠ T72), K74 (= K76), N95 (= N97), D110 (= D113), Q265 (= Q256)

3tozA 2.2 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with NAD.
28% identity, 93% coverage: 7:264/277 of query aligns to 9:276/291 of 3tozA

query
sites
3tozA

I

I

T

T

G

G

K

H

T

T

V

E

F

L

I

I

P

G

L

L

I

I

G

A

H

T

P

P

V

I

E

R

Q

H

V

S

K

L

S

S

P

P

G

T

P

M

V

H

N

N

A

E

W

A

F

F

S

A

D

K

N

L

D

G

V

L

G

D

A

Y

V

V

L

Y

I

L

P

A

V

F

D

E

I

V

F

G

P

D

E

K

K

E

V

L

P

K

A

D

F

V

L

V

D

Q

A

G

I

F

R

R

G

A

T

M

P

-

N

N

C

L

P

R

G

G

V

W

S

N

V

V

T

S

M

M

P

P

H

N

K

K

Q

T

A

N

A

I

C

H

A

K

G

Y

V

L

D

D

E

K

L

L

T

S

E

P

R

A

A

A

R

E

R

L

A

V

E

G

A

A

V

V

N

N

I

T

I

V

R

V

R

-

N

N

A

D

D

D

G

G

T

V

L

L

V

T

G

G

D

H

M

I

V

T

D

D

G

G

D

T

A

G

M

Y

V

M

A

R

A

A

L

L

A

K

K

E

N

A

G

G

V

H

G

D

V

I

R

I

G

G

K

K

T

K

V

M

L

T

V

I

V

C

G
|
G

A
|
A

G
|
G

A
|
A

G

A

M

T

A

A

I

I

I

C

Y

I

A

Q

L

A

A

A

E

L

A

D

G

G

A

V

A

K

T

E

I

I

V

S

V

I

I

F

E
x
N

R
|
R

D

K

-
x
D

-

D

-
x
F

R

Y

A

A

R

N

S

A

D

E

R

K

L

T

I

V

G

E

Q

K

L

I

H

N

-

S

-

K

-

T

-

D

-

C

-

K

-

A

-

Q

-

L

-

F

-

D

-

I

R

E

D

D

Y

H

P

E

Q

Q

L

L

Q

R

A

-

Y

-

A

-

F

-

L

-

P

K

D

E

D

I

A

A

D

E

V

S

D

V

I

I

A

F

I

T

N

N

A

A

S
x
T

P
x
G

A
x
V

G

G

M
|
M

D

K

P

P

A
x
F

D

E

P

G

H

E

P

T

-

L

F

L

P

P

L

S

E

A

R

D

L

M

T

L

K

R

A

P

E

E

-

L

I

I

V

V

A

S

D

D

A
x
V

V

V

T
x
Y

K

K

P

P

P

T

V

K

T

T

P

R

W

L

L

L

E

E

E

I

A

A

R

E

R

Q

G

G

I

C

A

Q

I

T

Q

L

T

N

G
|
G

A

L

E

G

M
|
M

T
x
M

L

L

A

W

Q

Q

L

G

P

A

T

K

Q

A

I

F

A

E

F

I

W

W

binding nicotinamide-adenine-dinucleotide: G137 (= G137), A138 (= A138), G139 (= G139), G140 (= G140), A141 (= A141), N161 (≠ E161), R162 (= R162), D164 (vs. gap), F166 (vs. gap), T210 (≠ S200), G211 (≠ P201), V212 (≠ A202), M214 (= M204), F217 (≠ A207), V238 (≠ A226), Y240 (≠ T228), G261 (= G249), M264 (= M252), M265 (≠ T253)

Q8Y9N5 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
28% identity, 93% coverage: 7:264/277 of query aligns to 9:276/291 of Q8Y9N5

query
sites
Q8Y9N5

I

I

T

T

G

G

K

H

T

T

V

E

F

L

I

I

P

G

L

L

I

I

G

A

H

T

P

P

V

I

E

R

Q

H

V
x
S

K
x
L

S
|
S

P

P

G

T

P

M

V

H

N

N

A

E

W

A

F

F

S

A

D

K

N

L

D

G

V

L

G

D

A

Y

V

V

L

Y

I

L

P

A

V

F

D

E

I

V

F

G

P

D

E

K

K

E

V

L

P

K

A

D

F

V

L

V

D

Q

A

G

I

F

R

R

G

A

T

M

P

-

N

N

C

L

P

R

G

G

V

W

S

N

V

V

T

S

M

M

P

P

H

N

K

K

Q

T

A

N

A

I

C

H

A

K

G

Y

V

L

D

D

E

K

L

L

T

S

E

P

R

A

A

A

R

E

R

L

A

V

E

G

A

A

V

V

N

N

I

T

I

V

R

V

R

-

N

N

A

D

D

D

G

G

T

V

L

L

V

T

G

G

D

H

M

I

V

T

D

D

G

G

D

T

A

G

M

Y

V

M

A

R

A

A

L

L

A

K

K

E

N

A

G

G

V

H

G

D

V

I

R

I

G

G

K

K

T

K

V

M

L

T

V

I

V

C

G

G

A

A

G

G

A

A

G

A

M

T

A

A

I

I

I

C

Y

I

A

Q

L

A

A

A

E

L

A

D

G

G

A

V

A

K

T

E

I

I

V

S

V

I

I

F

E
x
N

R
|
R

D
x
K

-
x
D

-

D

-

F

R

Y

A

A

R

N

S

A

D

E

R

K

L

T

I

V

G

E

Q

K

L

I

H

N

-

S

-

K

-

T

-

D

-

C

-

K

-

A

-

Q

-

L

-

F

-

D

-

I

R

E

D

D

Y

H

P

E

Q

Q

L

L

Q

R

A

-

Y

-

A

-

F

-

L

-

P

K

D

E

D

I

A

A

D

E

V

S

D

V

I

I

A

F

I

T

N

N

A

A

S

T

P

G

A

V

G

G

M
|
M

D

K

P

P

A

F

D

E

P

G

H

E

P

T

-

L

F

L

P

P

L

S

E

A

R

D

L

M

T

L

K

R

A

P

E

E

-

L

I

I

V

V

A

S

D

D

A

V

V

V

T

Y

K

K

P

P

P

T

V

K

T

T

P

R

W

L

L

L

E

E

E

I

A

A

R

E

R

Q

G

G

I

C

A

Q

I

T

Q

L

T

N

G

G

A

L

E

G

M

M

T

M

L

L

A

W

Q

Q

L

G

P

A

T

K

Q

A

I

F

A

E

F

I

W

W

SLS 26:28 (≠ VKS 24:26) binding shikimate
NRKD 161:164 (≠ ERD- 161:163) binding NAD(+)
M214 (= M204) binding NADP(+)

1nytA Shikimate dehydrogenase aroe complexed with NADP+ (see paper)
29% identity, 90% coverage: 16:264/277 of query aligns to 6:252/271 of 1nytA

query
sites
1nytA

L

V

I

F

G

G

H

N

P

P

V

I

E

A

Q

H

V

S

K

K

S

S

P

P

G

-

P

F

V

I

N

H

A

Q

W

Q

F

F

S

A

-

Q

-

L

-

N

-

I

D

E

N

H

D

P

V

Y

G

G

A

R

V

V

L

L

I

A

P

P

V

I

D

N

I

D

F

F

P

I

E

N

K

T

V

L

P

N

A

A

F

F

L

F

D

S

A

A

I

-

R

-

G

-

T

-

P

-

N

G

C

G

P

K

G

G

V

A

S

N

V

V

T

T

M

V

P

P

H

F

K
|
K

Q

E

A

E

A

A

C

F

A

A

G

R

V

A

D

D

E

E

L

L

T

T

E

E

R

R

A

A

R

A

R

L

A

A

E

G

A

A

V

V

N

N

I

T

I

L

R

M

R

R

N

L

A

E

D

D

G

G

T

R

L

L

V

L

G

G

D

D

M

N

V

T

D
|
D

G

G

D

V

A

G

M

L

V

L

A

S

A

D

L

L

A

E

K

R

N

L

G

S

V

F

G

I

V

R

R

P

G

G

K

L

T

R

V

I

L

L

V

L

V

I

G

G

A

A

G
|
G

A
|
A

G

S

M

R

A

G

I

V

I

L

Y

L

A

P

L

L

A

L

E

S

A

L

G

D

A

C

A

A

T

-

I

V

V

T

V

I

I

T

E
x
N

R
|
R

D
x
T

R

V

A

S

R
|
R

S

A

D

E

R

E

L

L

I

-

G

A

Q

K

L

L

H

F

R

A

D

H

Y

T

P

G

Q

S

L

I

Q

Q

A

A

Y

L

A

S

F

M

L

D

P

E

-

L

D

E

D

G

A

H

D

E

V

F

D

D

I

L

A

I

I

I

N

N

A

A

S
x
T

P
x
S

A
x
S

G

G

M

I

D

S

P

G

A

D

D

I

P

P

H

-

P

A

F

I

P

P

L

S

E

S

R

L

L

I

T

H

K

P

A

G

E

I

I

Y

V

C

A

Y

D

D

A
x
M

V

F

T

Y

K

Q

P

K

P

G

V

K

T

T

P

P

W

F

L

L

E

A

E

W

A

C

R

E

R

Q

R

R

G

G

I

S

A

K

I

R

Q

N

T

A

-

D

G
|
G

A

L

E

G

M
|
M

T
x
L

L

V

A

A

Q

Q

L

A

P

A

T

H

Q

A

I

F

A

L

F

L

W

W

binding nadp nicotinamide-adenine-dinucleotide phosphate: K65 (= K76), D102 (= D113), G128 (= G139), G129 (= G140), A130 (= A141), N149 (≠ E161), R150 (= R162), T151 (≠ D163), R154 (= R166), T188 (≠ S200), S189 (≠ P201), S190 (≠ A202), M213 (≠ A226), G237 (= G249), M240 (= M252), L241 (≠ T253)

Query Sequence

>WP_015888332.1 NCBI__GCF_000018545.1:WP_015888332.1
MPPVPEITGKTVFIPLIGHPVEQVKSPGPVNAWFSDNDVGAVLIPVDIFPEKVPAFLDAI
RGTPNCPGVSVTMPHKQAACAGVDELTERARRAEAVNIIRRNADGTLVGDMVDGDAMVAA
LAKNGVGVRGKTVLVVGAGGAGMAIIYALAEAGAATIVVIERDRARSDRLIGQLHRDYPQ
LQAYAFLPDDADVDIAINASPAGMDPADPHPFPLERLTKAEIVADAVTKPPVTPWLEEAR
RRGIAIQTGAEMTLAQLPTQIAFWGLDKAGGQEAKRR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory