SitesBLAST
Comparing WP_015888332.1 NCBI__GCF_000018545.1:WP_015888332.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4k28A 2.15 angstrom resolution crystal structure of a shikimate dehydrogenase family protein from pseudomonas putida kt2440 in complex with NAD+ (see paper)
41% identity, 93% coverage: 9:266/277 of query aligns to 2:262/266 of 4k28A
- binding manganese (ii) ion: C153 (≠ I160), C164 (≠ I171), V176 (≠ A183), F180 (≠ L187)
- binding nicotinamide-adenine-dinucleotide: I129 (≠ V136), G130 (= G137), G132 (= G139), G133 (= G140), V134 (≠ A141), C153 (≠ I160), D154 (≠ E161), P155 (≠ R162), R159 (= R166), S193 (= S200), P194 (= P201), V222 (≠ A226), G245 (= G249), M248 (= M252), A249 (≠ T253)
Q9KVT3 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
30% identity, 90% coverage: 16:264/277 of query aligns to 10:256/278 of Q9KVT3
- SKS 18:20 (≠ VKS 24:26) binding shikimate
- N90 (= N97) binding shikimate
- D106 (= D113) binding shikimate
- NRTFAK 154:159 (≠ ERDRAR 161:166) binding NADP(+)
- Q248 (= Q256) binding shikimate
3pgjA 2.49 angstrom resolution crystal structure of shikimate 5- dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate
30% identity, 90% coverage: 16:264/277 of query aligns to 6:252/272 of 3pgjA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S14 (≠ V24), S16 (= S26), N59 (≠ S70), T61 (= T72), K65 (= K76), N86 (= N97), D102 (= D113), Q244 (= Q256)
1nvtB Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
32% identity, 93% coverage: 7:264/277 of query aligns to 7:267/287 of 1nvtB
- active site: K75 (= K76), D111 (= D113)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I72 (≠ M73), G135 (= G137), G137 (= G139), G138 (= G140), A139 (= A141), N157 (vs. gap), R158 (vs. gap), T159 (≠ V159), K162 (≠ R162), A200 (= A199), T201 (≠ S200), P202 (= P201), I203 (≠ A202), M205 (= M204), L229 (≠ A226), Y231 (≠ T228), M255 (= M252), L256 (≠ T253)
- binding zinc ion: E22 (= E22), H23 (≠ Q23)
1nvtA Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
32% identity, 93% coverage: 7:264/277 of query aligns to 7:267/287 of 1nvtA
- active site: K75 (= K76), D111 (= D113)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G135 (= G137), A139 (= A141), N157 (vs. gap), R158 (vs. gap), T159 (≠ V159), K162 (≠ R162), A200 (= A199), T201 (≠ S200), P202 (= P201), I203 (≠ A202), M205 (= M204), L229 (≠ A226), Y231 (≠ T228), G252 (= G249), M255 (= M252), L256 (≠ T253)
Q5HNV1 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Staphylococcus epidermidis (strain ATCC 35984 / RP62A) (see paper)
29% identity, 90% coverage: 16:264/277 of query aligns to 5:247/269 of Q5HNV1
- SLS 13:15 (≠ VKS 24:26) binding shikimate
- T60 (= T72) binding shikimate
- N85 (= N97) binding shikimate
- D100 (= D113) binding shikimate
- Y211 (≠ T228) Plays a major role in the catalytic process and a minor role in the substrate binding; mutation to F: Leads to a 345-fold decrease in the catalytic efficiency and a 3-fold decrease in the affinity binding for shikimate.
- Q239 (= Q256) binding shikimate
Q58484 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
32% identity, 93% coverage: 7:264/277 of query aligns to 2:262/282 of Q58484
1npdB X-ray structure of shikimate dehydrogenase complexed with NAD+ from e.Coli (ydib) northeast structural genomics research consortium (nesg) target er24 (see paper)
28% identity, 94% coverage: 6:264/277 of query aligns to 2:270/288 of 1npdB
- binding nicotinamide-adenine-dinucleotide: A132 (= A138), G133 (= G139), G134 (= G140), A135 (= A141), N155 (≠ E161), R156 (= R162), D158 (≠ R164), F160 (≠ R166), T204 (≠ S200), K205 (≠ P201), V206 (≠ A202), M208 (= M204), C232 (≠ A226), M258 (= M252), L259 (≠ T253)
P0A6D5 Quinate/shikimate dehydrogenase; NAD-dependent shikimate 5-dehydrogenase; EC 1.1.1.282 from Escherichia coli (strain K12) (see 4 papers)
28% identity, 94% coverage: 6:264/277 of query aligns to 2:270/288 of P0A6D5
- S22 (= S26) mutation to A: Kinetically unchanged as compared with the wild-type.
- Y39 (≠ L43) mutation to F: Kinetically unchanged as compared with the wild-type.
- S67 (≠ T72) mutation to A: Reduces activity towards quinate about 6-fold, but has a little effect on shikimate conversion.
- K71 (= K76) mutation to A: 3200-fold decrease in the affinity for quinate. 170-fold decrease in the affinity for shikimate.; mutation to G: 10-fold greater reduction in catalytic efficiency is observed with quinate than with shikimate.
- N92 (= N97) mutation to A: Alters protein structure. Loss of activity for both substrates.
- T106 (≠ V112) mutation to A: 2000-fold decrease in the affinity for quinate. 70-fold decrease in the affinity for shikimate. 10-fold greater reduction in catalytic efficiency is observed with quinate than with shikimate.
- D107 (= D113) mutation to A: Loss of activity towards quinate. 20000-fold decrease in the affinity for shikimate.
- AGGA 132:135 (= AGGA 138:141) binding NAD(+)
- NRRD 155:158 (≠ ERDR 161:164) binding NAD(+)
- K205 (≠ P201) binding NAD(+)
- CVYN 232:235 (≠ AVTK 226:229) binding NAD(+)
- G255 (= G249) binding NAD(+)
- Q262 (= Q256) mutation to A: 3-fold reduction in catalytic efficiency for both substrates.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3sefA 2.4 angstrom resolution crystal structure of shikimate 5-dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate and NADPH
32% identity, 71% coverage: 68:264/277 of query aligns to 53:248/268 of 3sefA
3sefC 2.4 angstrom resolution crystal structure of shikimate 5-dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate and NADPH
33% identity, 69% coverage: 16:206/277 of query aligns to 6:192/244 of 3sefC
Sites not aligning to the query:
1o9bA Quinate/shikimate dehydrogenase ydib complexed with nadh (see paper)
28% identity, 91% coverage: 13:264/277 of query aligns to 3:264/280 of 1o9bA
- binding 1,4-dihydronicotinamide adenine dinucleotide: A126 (= A138), G127 (= G139), G128 (= G140), A129 (= A141), R150 (= R162), F154 (≠ R166), K199 (≠ P201), V200 (≠ A202), M202 (= M204), C226 (≠ A226), Y228 (≠ T228), M252 (= M252), L253 (≠ T253)
3dooA Crystal structure of shikimate dehydrogenase from staphylococcus epidermidis complexed with shikimate (see paper)
28% identity, 90% coverage: 16:264/277 of query aligns to 5:238/258 of 3dooA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S13 (≠ V24), S15 (= S26), N58 (≠ S70), T60 (= T72), K64 (= K76), N85 (= N97), D100 (= D113), F227 (≠ T253), Q230 (= Q256)
2hk9A Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
26% identity, 94% coverage: 7:265/277 of query aligns to 2:251/269 of 2hk9A
- binding 2'-monophosphoadenosine-5'-diphosphate: V67 (≠ M73), G132 (= G139), G133 (= G140), A134 (= A141), N153 (≠ D163), R154 (= R164), T155 (≠ A165), T188 (≠ S200), S189 (≠ P201), V190 (≠ A202)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (≠ V24), S21 (= S26), N64 (≠ S70), K70 (= K76), N91 (= N97), D106 (= D113), Y216 (≠ T228), L239 (≠ T253), Q242 (= Q256)
2hk9B Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
26% identity, 94% coverage: 7:265/277 of query aligns to 2:251/267 of 2hk9B
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V67 (≠ M73), G130 (= G137), G133 (= G140), A134 (= A141), N153 (≠ D163), R154 (= R164), T155 (≠ A165), K158 (≠ D168), T188 (≠ S200), S189 (≠ P201), V190 (≠ A202), I214 (≠ A226), M238 (= M252), L239 (≠ T253)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (≠ V24), S21 (= S26), N64 (≠ S70), T66 (= T72), K70 (= K76), N91 (= N97), D106 (= D113), Y216 (≠ T228), L239 (≠ T253), Q242 (= Q256)
O67049 Shikimate dehydrogenase (NADP(+)); SD; SDH; EC 1.1.1.25 from Aquifex aeolicus (strain VF5) (see paper)
26% identity, 94% coverage: 7:265/277 of query aligns to 2:251/269 of O67049
- SLS 19:21 (≠ VKS 24:26) binding shikimate
- D82 (≠ E88) binding NADP(+)
- N91 (= N97) binding shikimate
- D106 (= D113) binding shikimate
- GAGGA 130:134 (= GAGGA 137:141) binding NADP(+)
- I214 (≠ A226) binding NADP(+)
- Y216 (≠ T228) binding shikimate
- G235 (= G249) binding NADP(+)
- Q242 (= Q256) binding shikimate
3tnlA 1.45 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with shikimate and NAD.
28% identity, 93% coverage: 7:264/277 of query aligns to 6:273/288 of 3tnlA
- binding nicotinamide-adenine-dinucleotide: M71 (= M73), G134 (= G137), A135 (= A138), G136 (= G139), G137 (= G140), A138 (= A141), N158 (≠ E161), R159 (= R162), D161 (vs. gap), F163 (vs. gap), T207 (≠ S200), V209 (≠ A202), M211 (= M204), F214 (≠ A207), V235 (≠ A226), Y237 (≠ T228), M261 (= M252), M262 (≠ T253)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S23 (≠ V24), S25 (= S26), N68 (≠ S70), S70 (≠ T72), K74 (= K76), N95 (= N97), D110 (= D113), Q265 (= Q256)
3tozA 2.2 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with NAD.
28% identity, 93% coverage: 7:264/277 of query aligns to 9:276/291 of 3tozA
- binding nicotinamide-adenine-dinucleotide: G137 (= G137), A138 (= A138), G139 (= G139), G140 (= G140), A141 (= A141), N161 (≠ E161), R162 (= R162), D164 (vs. gap), F166 (vs. gap), T210 (≠ S200), G211 (≠ P201), V212 (≠ A202), M214 (= M204), F217 (≠ A207), V238 (≠ A226), Y240 (≠ T228), G261 (= G249), M264 (= M252), M265 (≠ T253)
Q8Y9N5 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
28% identity, 93% coverage: 7:264/277 of query aligns to 9:276/291 of Q8Y9N5
1nytA Shikimate dehydrogenase aroe complexed with NADP+ (see paper)
29% identity, 90% coverage: 16:264/277 of query aligns to 6:252/271 of 1nytA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K65 (= K76), D102 (= D113), G128 (= G139), G129 (= G140), A130 (= A141), N149 (≠ E161), R150 (= R162), T151 (≠ D163), R154 (= R166), T188 (≠ S200), S189 (≠ P201), S190 (≠ A202), M213 (≠ A226), G237 (= G249), M240 (= M252), L241 (≠ T253)
Query Sequence
>WP_015888332.1 NCBI__GCF_000018545.1:WP_015888332.1
MPPVPEITGKTVFIPLIGHPVEQVKSPGPVNAWFSDNDVGAVLIPVDIFPEKVPAFLDAI
RGTPNCPGVSVTMPHKQAACAGVDELTERARRAEAVNIIRRNADGTLVGDMVDGDAMVAA
LAKNGVGVRGKTVLVVGAGGAGMAIIYALAEAGAATIVVIERDRARSDRLIGQLHRDYPQ
LQAYAFLPDDADVDIAINASPAGMDPADPHPFPLERLTKAEIVADAVTKPPVTPWLEEAR
RRGIAIQTGAEMTLAQLPTQIAFWGLDKAGGQEAKRR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory