SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_015902166.1 NCBI__GCF_000021725.1:WP_015902166.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
55% identity, 96% coverage: 17:643/651 of query aligns to 16:640/648 of Q89WV5

query
sites
Q89WV5

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G263 (= G261) mutation to I: Loss of activity.
G266 (= G264) mutation to I: Great decrease in activity.
K269 (= K267) mutation to G: Great decrease in activity.
E414 (= E412) mutation to Q: Great decrease in activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
54% identity, 92% coverage: 47:643/651 of query aligns to 46:643/652 of P27550

query
sites
P27550

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K609 (= K610) modified: N6-acetyllysine; by autocatalysis

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
55% identity, 92% coverage: 47:643/651 of query aligns to 42:637/641 of 2p20A

query
sites
2p20A

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active site: T260 (= T259), T412 (= T411), E413 (= E412), N517 (= N520), R522 (= R525), K605 (= K610)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G382), E384 (= E383), P385 (= P384), T408 (= T407), W409 (= W408), W410 (= W409), Q411 (= Q410), T412 (= T411), D496 (= D499), I508 (= I511), R511 (= R514), R522 (= R525)

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
55% identity, 92% coverage: 47:643/651 of query aligns to 46:643/652 of Q8ZKF6

query
sites
Q8ZKF6

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R194 (≠ S191) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T306) binding CoA
N335 (≠ T330) binding CoA
A357 (= A352) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D516) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S522) binding CoA
G524 (= G523) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R525) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (≠ K585) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K610) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
55% identity, 92% coverage: 47:643/651 of query aligns to 42:636/640 of 5jrhA

query
sites
5jrhA

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|
E

A

A

F

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

A

P

E

E

A

A

L

V

K

A

D
x
G

R

R

I

I

L

I

D

D

A

S

E

S

A

S

K

R

V

L

V

V

I

I

T

T

A

A

D

D

G

E

A

G

F

V

R
|
R

K

A

G

G

S

R

P

S

Y

I

M

P

L

L

K

K

P

K

T

N

V

V

D

D

I

D

A

A

L

L

E

K

G

N

V

P

D

N

I

V

-

T

-

S

V

V

E

E

K

H

V

V

I

I

V

V

V

L

E

K

R

R

N

T

N

G

E

S

D

D

I

I

E

D

W

W

V

Q

S

E

G

G

R

R

D

D

I

L

S

W

Y

W

N

R

D

D

L

L

I

I

V

E

N

K

Q

A

S

S

D

P

E

E

C

H

P

Q

A

P

E

E

I

A

M

M

D

N

S

A

E

E

D

D

P

P

L

L

F

F

L

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

V

V

Q

L

H

H

A

T

Q

T

A

G

G

G

Y

Y

I

L

L

V

W

Y

A

A

Q

A

L

T

T

T

M

F

E

K

W

Y

V

V

F

F

D

D

I

Y

K

H

D

P

N

G

D

D

T

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

I

V

T

T

G

G

H

H

T

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

A

C

G

G

A

A

T

T

L

L

M

M

F

F

E

E

G

G

V

V

L

P

T

N

Y

W

P

P

D

T

S

P

G

A

R

R

A

M

W

C

K

Q

M

V

V

V

E

D

E

K

Y

H

K

Q

V

V

N

N

Q

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

L

A

L

L

N

M

K

A

M

E

G

G

P

D

D

K

E

A

P

I

K

E

K

G

Y

T

D

D

L

R

S

S

L

L

R

R

I

I

L

L

G

G

T

S

V

V

G
|
G

E
|
E

P
|
P

I

I

D

N

P

P

P

E

A

A

W

W

K

E

W

W

Y

Y

Y

W

E

K

V

K

V

I

G

G

G

K

G

E

R

K

C

C

S

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

M

M

I

I

T

T

P

P

L

L

P

P

A

G

A

A

T

I

P

E

V

L

K

K

P

A

A

G

S

S

A

A

T

T

F

R

P

P

L

F

P

F

G

G

I

V

F

Q

A

P

E

A

I

L

I

V

D

D

E

N

T

E

G

G

E

H

R

P

M

Q

P

E

A

G

H

A

E

T

K

E

G

G

L

N

L

L

C

V

I

I

T

T

K

D

P

S

W

W

P

P

S

G

M

Q

I

A

R

R

T

T

I

L

W

F

G

G

D

D

P

H

E

E

R

R

F

F

V

E

K

Q

S

T

Y

Y

F

F

S

S

T

T

A

F

K

K

K

N

M

M

G

-

E

-

P

-

V

-

Y

Y

F

F

S

S

G

G

D
|
D

G

G

A

A

M

R

Y

R

D

D

E

E

D

D

G

G

Y

Y

I

Y

W

W

I
|
I

T

T

G

G

R

R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

L

L

G

G

T

T

A

A

E

E

I

I

E

E

A

S

A

A

I

L

K
x
V

K

A

H
|
H

P

P

L

K

I
|
I

A

A

E

E

V

A

A

A

V

V

G

G

R

I

P

P

D

H

E

A

I

I

K

K

G

G

E

Q

S

A

V

I

F

Y

A

A

Y

Y

V

V

V

T

L

L

K

N

-

H

G

G

E

E

D

E

T

-

V

-

A

-

E

P

E

S

M

P

E

E

I

L

I

Y

K

A

E

E

I

V

N

R

E

N

I

W

V

V

K
x
R

E

K

E

E

I

I

G

G

A
x
P

I

L

A

A

K

T

V

P

D

D

T

V

V

L

A

H

F

W

V

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

A

K

I

I

A

A

K

A

G

G

E

D

A

-

I

-

T

L

Q

G

D

D

T

T

S

S

T

T

L

L

E

A

D

D

P

P

K

G

V

V

E

E

A

K

I

L

E

E

active site: T260 (= T259), T412 (= T411), E413 (= E412), N517 (= N520), R522 (= R525), K605 (= K610)
binding (r,r)-2,3-butanediol: W93 (= W95), E140 (= E141), G169 (≠ D170), K266 (= K265), P267 (= P266)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G382), E384 (= E383), P385 (= P384), T408 (= T407), W409 (= W408), W410 (= W409), Q411 (= Q410), T412 (= T411), D496 (= D499), I508 (= I511), N517 (= N520), R522 (= R525)
binding coenzyme a: F159 (= F160), G160 (= G161), G161 (= G162), R187 (= R188), S519 (= S522), R580 (≠ K585), P585 (≠ A590)
binding magnesium ion: V533 (≠ K536), H535 (= H538), I538 (= I541)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
54% identity, 92% coverage: 47:643/651 of query aligns to 41:633/637 of 2p2fA

query
sites
2p2fA

I

L

T

D

W

W

F

I

E

T

D

P

F

Y

K

Q

Q

K

T

V

L

K

D

N

T

T

S

S

N

F

A

A

P

P

-

G

-

N

-

V

F

S

Y

I

K

K

W

W

F

Y

V

E

G

D

G

G

K

T

L

L

N

N

V

L

A

A

Y

A

Q

N

C

C

I

L

D

D

R

R

H

H

L

L

E

Q

N

E

R

N

K

G

N

D

K

R

A

T

A

A

I

I

W

W

E

E

G

G

D

D

N

D

-

T

G

S

E

Q

K

S

R

K

I

H

I

I

T

S

Y

Y

L

R

E

E

L

L

Y

H

R

R

E

D

V

V

N

C

R

R

F

F

A

A

N

N

L

T

L

L

K

L

S

D

L

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

V

A

I

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

F

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

G

G

F

F

S

S

A

P

E

E

A

A

L

V

K

A

D

G

R

R

I

I

L

I

D

D

A

S

E

S

A

S

K

R

V

L

V

V

I

I

T

T

A

A

D

D

G

E

A

G

F

V

R
|
R

K

A

G

G

S

R

P

S

Y

I

M

P

L

L

K

K

P

K

T

N

V

V

D

D

I

D

A

A

L

L

E

K

G

N

V

P

D

N

I

V

-

T

-

S

V

V

E

E

K

H

V

V

I

I

V

V

V

L

E

K

R

R

N

T

N

G

E

S

D

D

I

I

E

D

W

W

V

Q

S

E

G

G

R

R

D

D

I

L

S

W

Y

W

N

R

D

D

L

L

I

I

V

E

N

K

Q

A

S

S

D

P

E

E

C

H

P

Q

A

P

E

E

I

A

M

M

D

N

S

A

E

E

D

D

P

P

L

L

F

F

L

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

Q

L

H

H

A

T

Q

T

A

G

G

G

Y

Y

I

L

L

V

W

Y

A

A

Q

A

L

T

T

T

M

F

E

K

W

Y

V

V

F

F

D

D

I

Y

K

H

D

P

N

G

D

D

T

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W
|
W

I

V

T
|
T

G

G

H

H

T

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

A

C

G

G

A

A

T

T

L

L

M

M

F

F

E

E

G

G

V

V

L
x
P

T

N

Y

W

P

P

D

T

S

P

G

A

R

R

A

M

W

C

K

Q

M

V

V

V

E

D

E

K

Y

H

K

Q

V

V

N

N

Q

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A
|
A

I

I

R

R

L

A

L

L

N

M

K

A

M

E

G

G

P

D

D

K

E

A

P

I

K

E

K

G

Y

T

D

D

L

R

S

S

L

L

R

R

I

I

L

L

G

G

T

S

V

V

G
|
G

E
|
E

P
|
P

I

I

D

N

P

P

P

E

A

A

W

W

K

E

W

W

Y

Y

Y

W

E

K

V

K

V

I

G

G

G

K

G

E

R

K

C

C

S

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

M

M

I

I

T

T

P

P

L

L

P

P

A

G

A

A

T

I

P

E

V

L

K

K

P

A

A

G

S

S

A

A

T

T

F

R

P

P

L

F

P

F

G

G

I

V

F

Q

A

P

E

A

I

L

I

V

D

D

E

N

T

E

G

G

E

H

R

P

M

Q

P

E

A

G

H

A

E

T

K

E

G

G

L

N

L

L

C

V

I

I

T

T

K

D

P

S

W

W

P

P

S

G

M

Q

I

A

R

R

T

T

I

L

W

F

G

G

D

D

P

H

E

E

R

R

F

F

V

E

K

Q

S

T

Y

Y

F

F

S

S

T

T

A

F

K

K

K

N

M

M

G

-

E

-

P

-

V

-

Y

Y

F

F

S

S

G

G

D
|
D

G

G

A

A

M

R

Y

R

D

D

E

E

D

D

G

G

Y

Y

I

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

L

L

G

G

T

T

A

A

E

E

I

I

E

E

A

S

A

A

I

L

K

V

K

A

H

H

P

P

L

K

I

I

A

A

E

E

V

A

A

A

V

V

G

G

R

I

P

P

D

H

E

A

I

I

K

K

G

G

E

Q

S

A

V

I

F

Y

A

A

Y

Y

V

V

V

T

L

L

K

N

-

H

G

G

E

E

D

E

T

-

V

-

A

-

E

P

E

S

M

P

E

E

I

L

I

Y

K

A

E

E

I

V

N

R

E

N

I

W

V

V

K
x
R

E

K

E

E

I

I

G

G

A
x
P

I

L

A

A

K

T

V

P

D

D

T

V

V

L

A

H

F

W

V

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

A

K

I

I

A

A

K

A

G

G

E

D

A

T

I

-

T

-

Q

-

D

-

T

S

S

S

T

T

L

L

E

A

D

D

P

P

K

G

V

V

E

E

A

K

I

L

E

E

active site: T259 (= T259), T411 (= T411), E412 (= E412), N516 (= N520), R521 (= R525), K604 (= K610)
binding adenosine monophosphate: G382 (= G382), E383 (= E383), P384 (= P384), T407 (= T407), W408 (= W408), W409 (= W409), Q410 (= Q410), T411 (= T411), D495 (= D499), I507 (= I511), R510 (= R514), N516 (= N520), R521 (= R525)
binding coenzyme a: F158 (= F160), R186 (= R188), W304 (= W304), T306 (= T306), P329 (≠ L329), A352 (= A352), A355 (= A355), S518 (= S522), R579 (≠ K585), P584 (≠ A590)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
54% identity, 92% coverage: 47:643/651 of query aligns to 42:630/634 of 1pg3A

query
sites
1pg3A

I

L

T

D

W

W

F

I

E

T

D

P

F

Y

K

Q

Q

K

T

V

L

K

D

N

T

T

S

S

N

F

A

A

P

P

-

G

-

N

-

V

F

S

Y

I

K

K

W

W

F

Y

V

E

G

D

G

G

K

T

L

L

N

N

V

L

A

A

Y

A

Q

N

C

C

I

L

D

D

R

R

H

H

L

L

E

Q

N

E

R

N

K

G

N

D

K

R

A

T

A

A

I

I

W

W

E

E

G

G

D

D

N

D

-

T

G

S

E

Q

K

S

R

K

I

H

I

I

T

S

Y

Y

L

R

E

E

L

L

Y

H

R

R

E

D

V

V

N

C

R

R

F

F

A

A

N

N

L

T

L

L

K

L

S

D

L

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

V

A

I

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

F

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

G

G

F

F

S

S

A

P

E

E

A

A

L

V

K

A

D

G

R

C

I

I

L

I

D

D

A

S

E

S

A

S

K

R

V

L

V

V

I

I

T

T

A

A

D

D

G

E

A

G

F

V

R
|
R

K

A

G

G

S
x
R

P

S

Y

I

M

P

L

L

K

K

P

K

T

N

V

V

D

D

I

D

A

A

L

L

E

K

G

N

V

P

D

N

I

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-

T

-

S

V

V

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E

K

H

V

V

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I

V

V

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R

N

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W

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L

L

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F

L

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L

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Y

Y

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|
T

S

S

G

G

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S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

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L

H

H

A

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Y

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A

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T

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x
P

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N

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Y

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P

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A
|
A

I

I

R

R

L

A

L

L

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M

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A

M

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G

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D

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D

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L

L

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I

L

L

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|
G

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|
E

P
|
P

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N

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|
T

W
|
W

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|
Q

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|
T

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|
E

T

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G

G

H

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M

M

I

I

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|
D

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|
R

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L

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G

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T

A

A

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I

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V

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|
H

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|
I

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A

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Y

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x
R

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G

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x
P

I

L

A

A

K

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L

L

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K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

A

K

I

I

A

A

K

A

G

G

E

D

A

-

I

-

T

-

Q

-

D

-

T

-

S

-

T

-

L

L

E

A

D

D

P

P

K

G

V

V

E

E

A

K

I

L

E

E

active site: T260 (= T259), T412 (= T411), E413 (= E412), N517 (= N520), R522 (= R525), K605 (= K610)
binding coenzyme a: F159 (= F160), G160 (= G161), R187 (= R188), R190 (≠ S191), A301 (= A300), T307 (= T306), P330 (≠ L329), A356 (= A355), S519 (= S522), R580 (≠ K585), P585 (≠ A590)
binding magnesium ion: V533 (≠ K536), H535 (= H538), I538 (= I541)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G382), E384 (= E383), P385 (= P384), T408 (= T407), W409 (= W408), W410 (= W409), Q411 (= Q410), T412 (= T411), D496 (= D499), R511 (= R514), R522 (= R525)

P9WQD1 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
51% identity, 98% coverage: 7:641/651 of query aligns to 12:647/651 of P9WQD1

query
sites
P9WQD1

Y

Y

P

P

K

P

E

A

M

H

F

F

K

A

D

E

P

H

A

A

F

N

K

A

N

R

M

A

C

-

E

-

Y

-

H

-

E

E

L

L

V

Y

E

R

E

E

F

A

E

E

K

E

D

D

Y

R

E

L

G

A

T

F

W

W

A

A

K

K

F

Q

A

A

R

-

E

N

K

R

I

L

T

S

W

W

F

T

E

T

D

P

F

F

K

T

Q

E

T

V

L

L

D

D

T

W

S

S

N

G

A

A

P

P

F

F

Y

A

K

K

W

W

F

F

V

V

G

G

K

E

L

L

N

N

V

V

A

A

Y

Y

Q

N

C

C

I

V

D

D

R

R

H

H

L

V

E

E

-

A

N

G

R

H

K

G

N

D

K

R

A

V

A

A

I

I

I

H

W

W

E

E

G

G

D

E

N

P

-

V

G

G

E

D

K

R

R

R

I

T

I

L

T

T

Y

Y

L

S

E

D

L

L

Y

L

R

A

E

E

V

V

N

S

R

K

F

A

A

A

N

N

L

A

L

L

K

T

S

D

L

L

G

G

V

L

K

V

K

A

G

G

D

D

R

R

V

V

V

A

I

I

Y

Y

M

L

P

P

M

L

I

I

P

P

E

E

A

A

A

V

F

I

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

L

G

G

A

I

I

M

H

H

S

S

V

V

F

F

G

G

F

F

S

T

A

A

E

A

A

A

L

L

K

Q

D

A

R

R

I

I

L

V

D

D

A

A

E

Q

A

A

K

K

V

L

I

I

T

T

A

A

D

D

G

G

A

Q

F

F

R

R

K

R

G

G

S

K

P

P

Y

S

M

P

L

L

K

K

P

A

T

A

V

A

D

D

I

E

A

A

L

L

E

A

G

A

V

I

D

P

-

D

-

C

I

S

V

V

E

E

K

H

V

V

I

L

V

V

E

R

R

R

N

T

N

G

E

I

D

E

I

M

E

A

W

W

V

S

S

E

G

G

R

R

D

D

I

L

S

W

Y

W

N

H

D

H

L

V

I

V

V

G

N

S

Q

A

S

S

D

P

E

A

C

H

P

T

A

P

E

E

I

P

M

F

D

D

S

S

E

E

D

H

P

P

L

L

F

F

L

L

Y

Y

T

T

S

S

G

G

S

T

T

T

G

G

K

K

P

P

K

K

G

G

V

I

Q

M

H

H

A

T

Q

S

A

G

G

G