SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_015926901.1 NCBI__GCF_000022085.1:WP_015926901.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 16 hits to proteins with known functional sites (download)

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
29% identity, 85% coverage: 4:503/590 of query aligns to 1:443/557 of P78753

query
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P78753

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S391 (≠ V452) modified: Phosphoserine

Sites not aligning to the query:

489 modified: Phosphoserine

1ct9A Crystal structure of asparagine synthetase b from escherichia coli (see paper)
28% identity, 84% coverage: 27:520/590 of query aligns to 27:433/497 of 1ct9A

query
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active site: L50 (= L50), N74 (= N75), G75 (= G76), T305 (≠ S347), R308 (≠ H348), E332 (≠ Q371), M366 (≠ L416)
binding adenosine monophosphate: L232 (= L266), L233 (= L267), S234 (= S268), S239 (= S273), A255 (≠ S292), V256 (≠ I293), D263 (≠ E305), M316 (≠ L356), S330 (= S369), G331 (= G370), E332 (≠ Q371)
binding glutamine: R49 (= R49), L50 (= L50), I52 (= I52), V53 (≠ L53), N74 (= N75), G75 (= G76), E76 (≠ C77), D98 (= D100)

Sites not aligning to the query:

active site: 1
binding glutamine: 1

P22106 Asparagine synthetase B [glutamine-hydrolyzing]; AS-B; EC 6.3.5.4 from Escherichia coli (strain K12) (see 2 papers)
28% identity, 88% coverage: 1:520/590 of query aligns to 1:453/554 of P22106

query
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P22106

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E

M

L

T

R

E

Q

S

A

D

-

W

-

C

-

E

-

A

-

V

-

L

-

A

-

T

-

L

L

R

E

K

D

A

S

V

V

E

K

R

S

R

H

L

L

V

M

S

S

D

D

V

V

P

P

V

Y

G

G

V

V

L

L

S

S

G

G

L

L

D

D

S

S

I

I

V

I

V

S

A

A

L

I

L

T

-

K

-

Y

-

A

-

R

-

V

-

E

-

D

-

Q

-

E

-

R

A

S

E

E

A

A

G

W

Q

W

R

P

G

Q

L

L

K

H

T

S

F

F

S

A

I

V

G

G

F

L

E

-

S

-

V

-

G

-

E

P

A

G

G

S

D

P

E

D

F

L

T

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Y

A

S

A

D

Q

I

E

I

V

A

A

E

N

R

H

F

L

A

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T

T

D

V

H

H

R

E

I

I

R

H

I

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D

T

S

V

A

Q

R

E

A

G

L

L

P

D

A

A

L

I

H

R

G

D

A

V

V

I

A

Y

A

H

M

I

S

E

E

T

P

Y

M

D

V

V

S

T

-

T

-

I

H

R

D

A

A

S

V

T

G

P

F

M

Y

Y

L

L

L

M

S

S

E

R

E

K

V

I

S

K

K

A

H

M

-

G

V

I

K

K

V

M

V

V

Q

L

S

S

G

G

Q
x
E

G

G

A

S

D

D

E

E

I

V

F

F

G

G

Y

Y

H

L

W

Y

Y

F

P

H

P

K

M

A

M

P

Q

N

S

A

T

K

D

E

A

L

V

-

A

-

D

-

Y

-

A

-

R

-

V

-

F

-

D

-

R

-

D

-

H

H

A

E

E

E

-

T

M

V

R

R

E

K

A

L

L

L

S

A

P

L

S

H

L

M

V

Y

D

D

G

-

D

-

V

-

S

-

R

-

A

-

F

-

V

-

E

-

A

-

H

-

F

-

A

-

R

-

A

-

G

-

A

-

P

-

R

-

P

-

I

-

D

-

K

-

A

-

L

-

R

-

I

-

D

-

T

-

E

-

I

-

M

-

L

-

V

-

D

-

P

-

V

C

K

A

R

R

V

A

D

N

N

K

M

A

T

M

M

S

A

A

W

W

G

G

L

V

E

E

A

A

R

R

V

V

P

P

F

F

L

L

D

D

H

K

D

K

L

F

V

L

E

D

L

V

A

A

G

M

R

R

V

I

P

N

A

P

D

Q

L

D

K

K

I

M

R

C

D

G

G

N

G

G

K

K

F

M

-

E

-

K

-

H

I

I

L

L

K

R

Q

E

A

C

G

F

R

E

R

A

V

Y

V

L

P

P

S

A

E

S

V

V

I

A

D

W

R

R

P

Q

K

K

G

E

Y

Q

F

F

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (≠ A29) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D33) mutation D->N,E: Little effect on the kinetic properties.
H81 (≠ F81) mutation to A: 5-fold decrease in glutamine affinity.
A105 (≠ K106) mutation to H: Little effect on the kinetic properties.
E349 (≠ Q371) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
25% identity, 88% coverage: 1:520/590 of query aligns to 1:470/561 of P08243

query
sites
P08243

M

M

C
|
C

G

G

I

I

C

W

G
x
A

E

L

I

F

R

G

L

S

D

D

G

-

A

-

P

-

D

D

A

C

A

L

A

S

V

V

A

Q

A

C

M

L

-

S

A

A

V

M

P

K

L

I

R

A

A

H

R

R

G

G

P

P

D

D

A

A

-

F

-

R

-

F

G

E

G

N

S

V

F

N

A

G

Q

Y

G

T

G

N

I

C

A

C

F

F

G

G

H

F

R

H

R

R

L

L

S

A

I

V

L

V

D

D

L

P

S

L

P

F

A

G

S

M

Q

Q

Q

P

P

I

M

R

V

V

D

K

P

K

G

Y

L

P

G

Y

L

L

A

W

I

L

V

C

F

Y

N

N

G

G

C

E

I

I

Y

Y

N

N

F

H

R

K

S

K

L

M

R

Q

E

Q

E

H

L

F

R

E

A

-

K

-

G

-

Y

F

A

E

F

Y

F

Q

S

T

E

K

G

V

D

D

T

G

E

E

V

I

I

I

L

L

K

H

A

L

Y

Y

H

D

A

K

W

G

G

G

-

I

P

E

R

Q

C

T

V

I

E

C

R

M

F

L

Y

D

G

G

M

V

F

F

A

A

F

F

V

V

L

L

W

L

E

D

R

T

D

A

S

N

G

K

R

K

V

V

V

F

I

L

A

G

R

R

D

D

R

T

L

Y

G

G

I

V

K

R

P

P

L

L

Y

F

Y

K

A

A

-

M

-

T

E

E

S

D

G

G

S

F

R

-

L

L

R

A

F

V

A

C

S

S

S

E

L

A

P

K

A

G

L

L

L

V

A

T

A

-

G

-

D

-

L

L

D

K

R

H

S

S

I

A

D

T

P

P

A

-

L

-

H

-

Y

F

M

L

S

K

F

V

H

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A

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V

F

V

L

P

P

A

G

P

H

L

Y

T

E

I

V

L

L

-

D

-

L

K

K

G

P

V

N

R

G

K

K

V

V

P

A

P

S

A

V

T

E

I

M

I

V

T

K

I

Y

E

H

P

H

D

C

G

R

R

D

R
x
V

R

P

Q

L

E

H

T

A

Y

L

W

Y

E

D

A

N

V

V

V

E

T

K

E

L

R

F

P

P

D

G

D

F

R

E

H

-

M

-

T

-

E

-

S

-

D

-

W

-

C

I

E

E

A

T

V

V

L

K

A

N

T

N

L

L

R

R

-

I

-

L

-

F

-

N

K

N

A

A

V

V

E

K

R

K

R

R

L

L

V

M

S

T

D

D

V

R

P

R

V

I

G

G

V

C

L

L

S

S

G

G

L

L

D

D

S

S

I

L

V

V

V

A

A

A

L

T

L

L

A

L

E

K

A

Q

G

L

Q

K

R

E

G

A

L

Q

K

V

T

Q

F

Y

S

P

I

L

G

Q

F

T

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F

S

A

V

I

G

G

G

M

E

E

A

D

G

S

D

P

E

D

F

L

T

L

Y

A

S

A

D

R

I

K

I

V

A

A

E

D

R

H

F

I

A

G

T

S

D

E

H

H

H

Y

R

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I

V

R

L

I

F

D

N

S

S

A

E

R

E

A

G

L

I

P

Q

A

A

L

L

H

D

G

E

A

V

V

I

A

F

A

S

M

L

S

E

E

T

P

Y

M

D

V

I

S

T

-

T

-

V

H

R

D

A

A

S

V

V

G

G

F

M

Y

Y

L

L

L

I

S

S

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K

E

Y

V

I

S

R

K

K

H

N

V

T

K

D

-

S

-

V

V

V

V

I

Q
x
F

S

S

G

G

Q

E

G

G

A

S

D

D

E

E

I

L

F

T

G

Q

G

G

Y

Y

H

-

W

-

Y

-

P

-

M

-

Q

-

S

-

T

-

D

-

A

-

V

-

A

-

D

-

Y

-

A

-

R

-

V

-

F

-

D

-

R

-

D

-

H

-

A

-

E

-

M

-

R

-

E

-

A

-

L

-

S

-

P

-

S

-

L

-

V

-

D

-

G

-

D

-

V

-

S

-

R

-

A

-

F

-

V

-

E

-

A

I

H

Y

F

F

A

H

R

K

A

A

G

P

A

S

P

P

R

E

P

K

I

A

D

E

K

E

A

E

L

S

R

E

I

R

D

L

T

L

E

R

I

E

M

L

L

Y

V

L

D

F

D

D

P

-

V

V

K

L

R

R

V

A

D

D

N

R

M

T

T

T

M

A

A

A

W

H

G

G

L

L

E

E

A

L

R

R

V

V

P

P

F

F

L

L

D

D

H

H

D

R

L

F

V

S

E

S

L

Y

A

Y

G

L

R

S

V

L

P

P

A

P

D

E

L

M

K

R

I

I

R

P

D

K

G

N

G

G

-

I

-

E

K

K

F

H

I

L

L

L

K

R

Q

E

A

T

-

F

-

E

G

D

R

S

R

N

V

L

V

I

P

P

S

K

E

E

V

I

I

L

D

W

R

R

P

P

K

K

G

E

Y

A

F

F

C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ G6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (≠ R217) to E: in dbSNP:rs1049674
F362 (≠ Q368) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
24% identity, 87% coverage: 5:519/590 of query aligns to 1:456/509 of 6gq3A

query
sites
6gq3A

C
|
C

G

G

E

I

I
x
W

R

A

L

L

D

F

G

G

A

S

P

D

D

C

A

L

A

S

A

V

V

Q

A

C

A

L

M

S

A

A

V

M

P

K

L

I

R

A

A

H

R

R

G

G

P

P

D

D

A

A

-

F

-

R

-

F

G

E

G

N

S

V

F

N

A

G

Q

Y

G

T

G

N

I

C

A

C

F

F

G

G

H

F

R

H

R
|
R

L
|
L

S

A

I
x
V

L
x
V

D

D

L

P

S

L

P

F

A

G

S

M

Q

Q

Q

P

P

I

M

R

V

V

D

K

P

K

G

Y

L

P

G

Y

L

L

A

W

I

L

V

C

F
x
Y

N
|
N

G
|
G

C
x
E

I

I

Y

Y

N

N

F

H

R

K

S

K

L

M

R

Q

E

Q

E

H

L

F

R

E

A

-

K

-

G

-

Y

F

A

E

F

Y

F

Q

S

T

E

K

G
x
V

D
|
D

T

G

E

E

V

I

I

I

L

L

K

H

A

L

Y

Y

H

D

A

K

W

G

G

G

-

I

P

E

R

Q

C

T

V

I

E

C

R

M

F

L

Y

D

G

G

M

V

F

F

A

A

F

F

V

V

L

L

W

L

E

D

R

T

D

A

S

N

G

K

R

K

V

V

V

F

I

L

A

G

R

R

D

D

R

T

L

Y

G

G

I

V

K

R

P

P

L

L

Y

F

Y

K

A

A

-

M

-

T

E

E

S

D

G

G

S

F

R

-

L

L

R

A

F

V

A

C

S

S

S

E

L

A

P

K

A

G

L

L

L

V

A

T

A

-

G

-

D

-

L

L

D

K

R

H

S

S

I

A

D

T

P

P

A

-

L

-

H

-

Y

F

M

L

S

K

F

V

H

E

A

P

V

F

V

L

P

P

A

G

P

H

L

Y

T

E

I

V

L

L

K

D

G

-

V

-

R

-

K

-

V

-

P

-

A

-

T

-

I

-

T

-

I

L

E

K

P

P

D

N

G

G

R

K

R

V

R

A

Q

S

E

-

T

-

Y

-

W

-

E

-

A

V

V

E

V

M

T

V

E

K

R

Y

P

H

D

H

D

C

R

R

H

D

M

V

T

F

E

P

S

G

D

F

W

E

C

I

E

E

A

T

V

V

L

K

A

N

T

N

L

L

R

R

-

I

-

L

-

F

-

N

K

N

A

A

V

V

E

K

R

K

R

R

L

L

V

M

S

T

D

D

V

R

P

R

V

I

G

G

V

C

L

L

S

S

G

G

L

L

D

D

S

S

I

L

V

V

V

A

A

A

L

T

L

L

A

L

E

K

A

Q

G

L

Q

K

R

E

G

A

L

Q

K

V

T

Q

F

Y

S

P

I

L

G

Q

F

T

E

F

S

A

V

I

G

G

G

M

E

E

A

D

G

S

D

P

E

D

F

L

T

L

Y

A

S

A

D

R

I

K

I

V

A

A

E

D

R

H

F

I

A

G

T

S

D

E

H

H

H

Y

R

E

I

V

R

L

I

F

D

N

S

S

A

E

R

E

A

G

L

I

P

Q

A

A

L

L

H

D

G

E

A

V

V

I

A

F

A

S

M

L

S

E

E

T

P

Y

M

D

V

I

S
x
T

-

T

-

V

H
x
R

D

A

A

S

V

V

G

G

F

M

Y

Y

L

L

L

I

S

S

E

K

E

Y

V

I

S

R

K

K

H

N

V

T

K

D

-

S

-

V

V

V

V

I

Q

F

S

S

G

G

Q

E

G

G

A

S

D

D

E

E

I

L

F

T

G

Q

G

G

Y

Y

H

-

W

-

Y

-

P

-

M

-

Q

-

S

-

T

-

D

-

A

-

V

-

A

-

D

-

Y

-

A

-

R

-

V

-

F

-

D

-

R

-

D

-

H

-

A

-

E

-

M

-

R

-

E

-

A

-

L

-

S

-

P

-

S

-

L

-

V

-

D

-

G

-

D

-

V

-

S

-

R

-

A

-

F

-

V

-

E

-

A

I

H

Y

F

F

A

H

R

K

A

A

G

P

A

S

P

P

R

E

P

K

I

A

D

E

K

E

A

E

L

S

R

E

I

R

D

L

T

L

E

R

I

E

M

L

L

Y

V

L

D

F

D

D

P

-

V

V

K

L

R

R

V

A

D

D

N

R

M

T

T

T

M

A

A

A

W

H

G

G

L

L

E

E

A

L

R

R

V

V

P

P

F

F

L

L

D

D

H

H

D

R

L

F

V

S

E

S

L

Y

A

Y

G

L

R

S

V

L

P

P

A

P

D

E

L

M

K

R

I

I

R

P

D

K

G

N

G

G

-

I

-

E

K

K

F

H

I

L

L

L

K

R

Q

E

A

T

-

F

-

E

G

D

R

S

R

N

V

L

V

I

P

P

S

K

E

E

V

I

I

L

D

W

R

R

P

P

K

S

G

W

Y

F

active site: W4 (≠ I8), L49 (= L50), N74 (= N75), G75 (= G76), T324 (≠ S347), R327 (≠ H348)
binding 5-oxo-l-norleucine: C1 (= C5), R48 (= R49), V51 (≠ I52), V52 (≠ L53), Y73 (≠ F74), N74 (= N75), G75 (= G76), E76 (≠ C77), V95 (≠ G99), D96 (= D100)

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
29% identity, 52% coverage: 73:381/590 of query aligns to 67:347/496 of 1mbzA

query
sites
1mbzA

V

V

F

L

N
x
A

G
|
G

C

E

I

I

Y

Y

N

N

F

R

R

D

S

E

L

L

R

L

E

S

E

V

L

L

R

P

A

A

K

-

G

G

Y

P

A

A

F

-

S

P

E

E

G

G

D

D

T

A

E

E

V

L

I

V

L

L

K

R

A

L

Y

L

H

E

A

R

W

Y

G

D

P

L

R

H

C

A

V

F

E

R

R

L

F

V

Y

N

G

G

M

R

F

F

A

A

F

T

V

V

L

V

W

-

E

-

R

R

D

T

S

G

G

D

R

R

V

V

V

L

I

L

A

A

R

T

D

D

R

H

L

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

A

C

E

V

S

A

G

P

S

G

R

E

L

V

R

R

F

A

A

S

S

T

S

E

L

A

P

K

A

A

L

L

L

A

A

A

A

H

G

G

D

-

L

-

D

-

R

-

S

-

I

-

D

-

P

-

A

-

L

-

H

-

Y

-

M

-

S

-

F

F

H

P

A

L

V

A

V

D

P

A

A

R

P

R

L

V

T

A

I

G

L

L

K

T

G

G

V

V

R

Y

K

Q

V

V

P

P

P

A

A

G

T

A

I

V

I

M

T

D

I

I

E

D

-

L

P

G

D

S

G

G

R

T

R

A

R

V

Q

T

E

H

T

R

Y

T

W

W

E

T

A

P

V

G

V

L

T

S

E

R

R

R

P

I

D

-

R

-

H

-

M

L

T

P

E

E

S

G

D

E

W

A

C

V

E

A

A

A

V

V

L

R

A

A

T

A

L

L

R

E

K

K

A

A

V

V

E

A

R

Q

R

R

L

V

V

T

-

P

S

G

D

D

V

T

P

P

V

L

G

-

V

V

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

I

G

V

V

V

A

A

A

L

C

L

A

A

H

E

R

A

A

G

A

Q

G

R

E

G

-

L

L

K

D

T

T

F

V

S
|
S

I
x
M

G

G

F

T

E

D

S

T

V

-

G

-

E

-

A

-

G

S

D

N

E

E

F

F

T

R

Y

E

S

A

D

R

I

A

I

V

A

V

E

D

R

H

F

L

A

R

T

T

D

R

H

H

H

R

R

E

I

I

R

T

I

I

D

P

S

T

A

T

R

E

A

L

L

L

P

A

A

Q

L

L

H

P

G

Y

A

A

V

V

A

W

A

A

M

S

S

E

E

S

P

-

M

-

V

V

S

D

H

P

D
|
D

A

I

V

I

G

E

F
x
Y

Y

L

L

L

-

P

-
x
L

-

T

-

A

L

L

S

Y

E

R

E

A

V

L

S

D

K

G

H

P

V

E

K

R

V

R

V

I

Q

L

S

T

G
|
G

Q
x
Y

G
|
G

A

A

D
|
D

E
x
I

I

P

F

L

G

G

Y

M

H

H

active site: A69 (≠ N75), G70 (= G76), D311 (= D349), Y337 (≠ Q371)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ L266), L237 (= L267), S238 (= S268), S243 (= S273), S261 (= S292), M262 (≠ I293), Y315 (≠ F353), L319 (vs. gap), G336 (= G370), Y337 (≠ Q371), G338 (= G372), D340 (= D374), I341 (≠ E375)
binding magnesium ion: D242 (= D272), D340 (= D374)
binding pyrophosphate 2-: S238 (= S268), G240 (= G270), D242 (= D272), S243 (= S273), D340 (= D374)

Sites not aligning to the query:

active site: 371, 432
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: 362, 371, 432, 434, 435
binding pyrophosphate 2-: 412, 432, 433

1jgtB Crystal structure of beta-lactam synthetase (see paper)
29% identity, 52% coverage: 73:381/590 of query aligns to 71:355/500 of 1jgtB

query
sites
1jgtB

V

V

F

L

N
x
A

G
|
G

C

E

I

I

Y

Y

N

N

F

R

R

D

S

E

L

L

R

L

E

S

E

V

L

L

R

P

A

A

K

-

G

G

Y

P

A

A

F

-

S

P

E

E

G

G

D

D

T

A

E

E

V

L

I

V

L

L

K

R

A

L

Y

L

H

E

A

R

W

Y

G

D

P

L

R

H

C

A

V

F

E

R

R

L

F

V

Y

N

G

G

M

R

F

F

A

A

F

T

V

V

L

V

W

-

E

-

R

R

D

T

S

G

G

D

R

R

V

V

V

L

I

L

A

A

R

T

D

D

R

H

L

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

A

C

E

V

S

A

G

P

S

G

R

E

L

V

R

R

F

A

A

S

S

T

S

E

L

A

P

K

A

A

L

L

L

A

A

A

A

H

G

R

D

D

L

-

D

-

R

-

S

-

I

-

D

-

P

-

A

-

L

-

H

-

Y

P

M

K

S

G

F

F

H

P

A

L

V

A

V

D

P

A

A

R

P

R

L

V

T

A

I

G

L

L

K

T

G

G

V

V

R

Y

K

Q

V

V

P

P

P

A

A

G

T

A

I

V

I

M

T

D

I

I

E

D

-

L

P

G

D

S

G

G

R

T

R

A

R

V

Q

T

E

H

T

R

Y

T

W

W

E

T

A

P

V

G

V

L

T

S

E

R

R

R

P

I

D

-

R

-

H

-

M

L

T

P

E

E

S

G

D

E

W

A

C

V

E

A

A

A

V

V

L

R

A

A

T

A

L

L

R

E

K

K

A

A

V

V

E

A

R

Q

R

R

L

V

V

T

-

P

S

G

D

D

V

T

P

P

V

L

G

-

V

V

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L
x
I

D
|
D

S
|
S

S

S

I

G

V

V

V

A

A

A

L

C

L

A

A

H

E

R

A

A

G

A

Q

G

R

E

G

-

L

L

K

D

T

T

F

V

S
|
S

I
x
M

G

G

F

T

E

D

S

T

V

-

G

-

E

-

A

-

G

S

D

N

E

E

F

F

T

R

Y

E

S

A

D

R

I

A

I

V

A

V

E

D

R

H

F

L

A

R

T

T

D

R

H

H

H

R

R

E

I

I

R

T

I

I

D

P

S

T

A

T

R

E

A

L

L

L

P

A

A

Q

L

L

H

P

G

Y

A

A

V

V

A

W

A

A

M

S

S

E

E

S

P

-

M

-

V

V

S

D

H

P

D
|
D

A

I

V

I

G

E

F
x
Y

Y

L

L

L

-

P

-
x
L

-

T

-

A

L

L

S

Y

E

R

E

A

V

L

S

D

K

G

H

P

V

E

K

R

V

R

V

I

Q

L

S

T

G
|
G

Q
x
Y

G
|
G

A

A

D
|
D

E
x
I

I

P

F

L

G

G

Y
x
M

H

H

active site: A73 (≠ N75), G74 (= G76), D319 (= D349), Y345 (≠ Q371)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ L266), L245 (= L267), S246 (= S268), G248 (= G270), I249 (≠ L271), D250 (= D272), S251 (= S273), S269 (= S292), M270 (≠ I293), L327 (vs. gap), G344 (= G370), Y345 (≠ Q371), D348 (= D374)
binding n2-(carboxyethyl)-l-arginine: Y323 (≠ F353), Y345 (≠ Q371), G346 (= G372), D348 (= D374), I349 (≠ E375), M354 (≠ Y380)
binding magnesium ion: D250 (= D272), D348 (= D374)

Sites not aligning to the query:

active site: 379, 440
binding diphosphomethylphosphonic acid adenosyl ester: 420, 440
binding n2-(carboxyethyl)-l-arginine: 370, 379

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
29% identity, 52% coverage: 73:381/590 of query aligns to 63:342/491 of 1mc1A

query
sites
1mc1A

V

V

F

L

N
x
A

G
|
G

C

E

I

I

Y

Y

N

N

F

R

R

D

S

E

L

L

R

L

E

S

E

V

L

L

R

P

A

A

K

-

G

G

Y

P

A

A

F

-

S

P

E

E

G

G

D

D

T

A

E

E

V

L

I

V

L

L

K

R

A

L

Y

L

H

E

A

R

W

Y

G

D

P

L

R

H

C

A

V

F

E

R

R

L

F

V

Y

N

G

G

M

R

F

F

A

A

F

T

V

V

L

V

W

-

E

-

R

R

D

T

S

G

G

D

R

R

V

V

V

L

I

L

A

A

R

T

D

D

R

H

L

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

A

C

E

V

S

A

G

P

S

G

R

E

L

V

R

R

F

A

A

S

S

T

S

E

L

A

P

K

A

A

L

L

L

A

A

A

A

G

G

F

D

P

L

L

D

A

R

D

S

A

I

R

D

R

P

V

A

A

A

G

L

L

H

-

Y

-

M

-

S

-

F

-

H

-

A

-

V

-

P

-

A

-

P

-

L

-

T

-

I

-

L

-

K

T

G

G

V

V

R

Y

K

Q

V

V

P

P

P

A

A

G

T

A

I

V

I

M

T

D

I

I

E

D

-

L

P

G

D

S

G

G

R

T

R

A

R

V

Q

T

E

H

T

R

Y

T

W

W

E

T

A

P

V

G

V

L

T

S

E

R

R

R

P

I

D

-

R

-

H

-

M

L

T

P

E

E

S

G

D

E

W

A

C

V

E

A

A

A

V

V

L

R

A

A

T

A

L

L

R

E

K

K

A

A

V

V

E

A

R

Q

R

R

L

V

V

T

-

P

S

G

D

D

V

T

P

P

V

L

G

-

V

V

L
x
V

L

L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

I

G

V

V

V

A

A

A

L

C

L

A

A

H

E

R

A

A

G

A

Q

G

R

E

G

-

L

L

K

D

T

T

F

V

S
|
S

I
x
M

G

G

F

T

E

D

S

T

V

-

G

-

E

-

A

-

G

S

D

N

E

E

F

F

T

R

Y

E

S

A

D

R

I

A

I

V

A

V

E

D

R

H

F

L

A

R

T

T

D

R

H

H

H

R

R

E

I

I

R

T

I

I

D

P

S

T

A

T

R

E

A

L

L

L

P

A

A

Q

L

L

H

P

G

Y

A

A

V

V

A

W

A

A

M

S

S

E

E

S

P

-

M

-

V

V

S

D

H

P

D
|
D

A

I

V

I

G

E

F
x
Y

Y

L

L

L

-

P

-

L

-

T

-

A

L

L

S

Y

E

R

E

A

V

L

S

D

K

G

H

P

V

E

K

R

V

R

V

I

Q

L

S

T

G
|
G

Q
x
Y

G
|
G

A

A

D
|
D

E
x
I

I

P

F

L

G

G

Y

M

H

H

active site: A65 (≠ N75), G66 (= G76), D306 (= D349), Y332 (≠ Q371)
binding adenosine monophosphate: V231 (≠ L266), S233 (= S268), S238 (= S273), S256 (= S292), M257 (≠ I293), G331 (= G370)
binding magnesium ion: D237 (= D272), D335 (= D374)
binding deoxyguanidinoproclavaminic acid: Y310 (≠ F353), Y332 (≠ Q371), G333 (= G372), I336 (≠ E375)
binding pyrophosphate 2-: S233 (= S268), G235 (= G270), D237 (= D272), S238 (= S273), D335 (= D374)

Sites not aligning to the query:

active site: 366, 427
binding adenosine monophosphate: 427, 430
binding deoxyguanidinoproclavaminic acid: 357, 366, 427
binding pyrophosphate 2-: 407, 427, 428

1mb9A Beta-lactam synthetase complexed with atp (see paper)
30% identity, 52% coverage: 73:381/590 of query aligns to 68:346/485 of 1mb9A

query
sites
1mb9A

V

V

F

L

N
x
A

G
|
G

C

E

I

I

Y

Y

N

N

F

R

R

D

S

E

L

L

R

L

E

S

E

V

L

L

R

P

A

A

K

-

G

G

Y

P

A

A

F

-

S

P

E

E

G

G

D

D

T

A

E

E

V

L

I

V

L

L

K

R

A

L

Y

L

H

E

A

R

W

Y

G

D

P

L

R

H

C

A

V

F

E

R

R

L

F

V

Y

N

G

G

M

R

F

F

A

A

F

T

V

V

L

V

W

-

E

-

R

R

D

T

S

G

G

D

R

R

V

V

V

L

I

L

A

A

R

T

D

D

R

H

L

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

-

A

-

E

-

S

-

G

-

S

-

R

-

L

-

R

-

F

-

A

-

S

-

L

-

P

-

A

T

L

C

L

V

A

A

A

P

G

G

D

E

L

V

D

R

R

A

S

S

I

T

D

E

P

A

A

K

A

A

L

L

H

A

Y

F

M

P

S

L

F

A

H

D

A

A

V

R

P

V

A

A

P

G

L

L

T

T

I

-

L

-

K

-

G

G

V

V

R

Y

K

Q

V

V

P

P

P

A

A

G

T

A

I

V

I

M

T

D

I

I

E

D

-

L

P

G

D

S

G

G

R

T

R

A

R

V

Q

T

E

H

T

R

Y

T

W

W

E

T

A

P

V

G

V

L

T

S

E

R

R

R

P

-

D

-

R

-

H

I

M

L

T

P

E

E

S

G

D

E

W

A

C

V

E

A

A

A

V

V

L

R

A

A

T

A

L

L

R

E

K

K

A

A

V

V

E

A

R

Q

R

R

L

V

V

T

-

P

S

G

D

D

V

T

P

P

V

L

G

-

V

V

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

I

G

V

V

V

A

A

A

L

C

L

A

A

H

E

R

A

A

G

A

Q

G

R

E

G

-

L

L

K

D

T

T

F

V

S
|
S

I
x
M

G

G

F

T

E

D

S

T

V

-

G

-

E

-

A

-

G

S

D

N

E

E

F

F

T

R

Y

E

S

A

D

R

I

A

I

V

A

V

E

D

R

H

F

L

A

R

T

T

D

R

H

H

H

R

R

E

I

I

R

T

I

I

D

P

S

T

A

T

R

E

A

L

L

L

P

A

A

Q

L

L

H

P

G

Y

A

A

V

V

A

W

A

A

M

S

S

E

E

S

P

-

M

-

V

V

S

D

H

P

D
|
D

A

I

V

I

G

E

F
x
Y

Y

L

L

L

-

P

-
x
L

-

T

-

A

L

L

S

Y

E

R

E

A

V

L

S

D

K

G

H

P

V

E

K

R

V

R

V

I

Q

L

S

T

G
|
G

Q
x
Y

G

G

A

A

D
|
D

E

I

I

P

F

L

G

G

Y

M

H

H

active site: A70 (≠ N75), G71 (= G76), D310 (= D349), Y336 (≠ Q371)
binding adenosine monophosphate: V235 (≠ L266), L236 (= L267), S242 (= S273), S260 (= S292), M261 (≠ I293), Y314 (≠ F353), L318 (vs. gap), G335 (= G370), Y336 (≠ Q371)
binding adenosine-5'-triphosphate: V235 (≠ L266), L236 (= L267), S237 (= S268), G239 (= G270), D241 (= D272), S242 (= S273), S260 (= S292), M261 (≠ I293), L318 (vs. gap), G335 (= G370), D339 (= D374)
binding magnesium ion: D241 (= D272), D339 (= D374)
binding pyrophosphate 2-: S237 (= S268), G239 (= G270), D241 (= D272), S242 (= S273), D339 (= D374)

Sites not aligning to the query:

active site: 370, 431
binding adenosine-5'-triphosphate: 411, 431
binding pyrophosphate 2-: 411, 431

1q19A Carbapenam synthetase (see paper)
22% identity, 72% coverage: 91:517/590 of query aligns to 72:442/500 of 1q19A

query
sites
1q19A

K

E

G

G

Y

E

A

A

F

Y

F

L

S

A

E

N

G

-

D

D

T

A

E

E

V

L

I

L

L

A

K

L

A

L

Y

F

H

T

A

R

W

L

G

G

P

A

R

N

C

A

V

L

E

A

R

L

F

A

Y

E

G

G

M

D

F

F

A

C

F

F

V

F

L

I

W

D

E

E

R

P

D

N

S

G

G

E

R

L

V

T

V

V

I

I

A

T

R

E

D

S

R

R

L

-

G

G

I

F

K

S

P

P

L

V

Y

H

Y

V

A

V

E

Q

S

-

G

G

S

K

R

K

L

A

R

W

F

M

A

T

S

N

S

S

L

L

P

K

A

L

L

V

L

T

A

A

-

E

G

G

D

E

L

G

D

A

R

L

S

W

I

F

D

E

P

E

A

E

A

A

L

L

H

V

H

C

Y

Q

M

S

S

L

F

M

H

R

A

A

V

D

V

T

P

Y

A

T

P

P

L

V

T

-

I

-

L

-

K

K

G

N

V

A

R

Q

K

R

V

L

P

K

P

P

A

G

T

A

I

V

I

H

T

V

I

L

E

T

P

H

D

D

G

S

R

E

R

G

R

Y

Q

-

E

-

T

S

Y

F

W

V

E

E

A

S

V

R

V

T

T

L

E

T

R

T

P

P

D

A

D

S

R

N

H

Q

M

L

T

L

E

A

S

L

D

P

W

R

C

-

E

E

A

P

V

L

L

L

A

A

T

L

L

I

R

D

K

R

A

Y

V

L

E

N

R

A

R

P

L

L

V

E

S

D

D

L

V

A

P

P

-

R

-

F

-

D

-

T

V

V

G

G

V

I

L
x
P

L
|
L

S
|
S

G

G

L

L

D
|
D

S
|
S

S

S

I

L

V

V

V

T

A

A

L

L

L

A

A

S

E

R

A

H

G

F

Q

K

R

K

G

-

L

L

K

N

T

T

F

Y

S
|
S

I
|
I

G

-

F

-

E

-

S

-

V

-

G

G

G

T

E

E

A

L

G

S

D

N

E

E

F

F

T

E

Y

F

S

S

D

Q

I

Q

I

V

A

A

E

D

R

A

F

L

A

G

T

T

D

-

H

H

R

Q

I

M

R

K

I

I

D

L

S

S

A

E

R

T

A

E

L

V

P

-

A

-

L

I

H

N

G

G

A

I

V

I

A

E

A

S

M

I

S

Y

-

Y

-

N

-

E

-

I

-

F

E

D

P

G

M
x
L

V

S

S

A

H
x
E

D

I

A

Q

V

S

G

G

F

L

Y

F

L

N

L

V

S

Y

E

R

E

Q

V

A

S

Q

K

G

H

Q

V

V

K

S

V

C

V

M

Q

L

S
x
T

G
|
G

Q
x
Y

G
|
G

A

S

D
|
D

E
x
L

I

L

F

F

G

G

Y

I

H

-

W

-

Y

L

P

K

P

P

M

G

M

A

Q

Q

S

-

T

-

D

-

A

-

V

-

A

-

D

-

Y

-

A

-

R

-

V

-

F

-

F

Y

D

D

R

N

D

P

H

N

A

Q

E

L

M

L

R

A

E

E

A

-

L

-

S

-

P

-

S

-

L

-

V

-

D

-

G

-

D

Q

V

V

S

Y

R
|
R

A

T

F

R

V

W

E

T

A

G

H
x
E

F

F

A

A

R

T

A

H

G

G

A

A

P

S

R

-

P

-

I

-

D

-

K

-

A

-

L

-

R

-

I

-

D

-

T

-

E

-

I

-

M

-

L

-

V

-

D

-

P

-

V

-

K

-

R

-

V

-

D

-

N

-

M

-

T

-

M

-

A

C

W

Y

G

G

L

I

E

D

A

I

R

R

V

H

P

P

F

F

L

W

D

S

H

H

D

S

L

L

V

I

E

S

L

L

A

C

G

H

R

A

V

L

P

H

A

P

D

D

L

Y

K

K

I

I

R

F

D

D

G

N

G

E

-

V

K

K

F

N

I

I

L

L

K

R

Q

E

A

Y

G

A

R

D

-

S

-

L

R

Q

V

L

P

P

S

K

E

D

V

I

I

V

D

W

R

R

P

K

K
|
K

active site: L318 (≠ M345), E321 (≠ H348), Y344 (≠ Q371), E379 (≠ H429), K442 (= K517)
binding diphosphomethylphosphonic acid adenosyl ester: P243 (≠ L266), L244 (= L267), S245 (= S268), D249 (= D272), S250 (= S273), S268 (= S292), I269 (= I293), T342 (≠ S369), G343 (= G370), D347 (= D374), K442 (= K517)
binding (2s,5s)-5-carboxymethylproline: Y344 (≠ Q371), G345 (= G372), L348 (≠ E375), R373 (= R423), E379 (≠ H429)

Sites not aligning to the query:

active site: 56
binding diphosphomethylphosphonic acid adenosyl ester: 443, 444, 445

Q9XB61 Carbapenam-3-carboxylate synthase; Carbapenam-3-carboxylate ligase; EC 6.3.3.6 from Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora) (see 3 papers)
22% identity, 72% coverage: 91:517/590 of query aligns to 73:443/503 of Q9XB61

query
sites
Q9XB61

K

E

G

G

Y

E

A

A

F

Y

F

L

S

A

E

N

G

-

D

D

T

A

E

E

V

L

I

L

L

A

K

L

A

L

Y

F

H

T

A

R

W

L

G

G

P

A

R

N

C

A

V

L

E

A

R

L

F

A

Y

E

G

G

M

D

F

F

A

C

F

F

V

F

L

I

W

D

E

E

R

P

D

N

S

G

G

E

R

L

V

T

V

V

I

I

A

T

R

E

D

S

R

R

L

-

G

G

I

F

K

S

P

P

L

V

Y

H

Y

V

A

V

E

Q

S

-

G

G

S

K

R

K

L

A

R

W

F

M

A

T

S

N

S

S

L

L

P

K

A

L

L

V

L

T

A

A

-

E

G

G

D

E

L

G

D

A

R

L

S

W

I

F

D

E

P

E

A

E

A

A

L

L

H

V

H

C

Y

Q

M

S

S

L

F

M

H

R

A

A

V

D

V

T

P

Y

A

T

P

P

L

V

T

-

I

-

L

-

K

K

G

N

V

A

R

Q

K

R

V

L

P

K

P

P

A

G

T

A

I

V

I

H

T

V

I

L

E

T

P

H

D

D

G

S

R

E

R

G

R

Y

Q

-

E

-

T

S

Y

F

W

V

E

E

A

S

V

R

V

T

T

L

E

T

R

T

P

P

D

A

D

S

R

N

H

Q

M

L

T

L

E

A

S

L

D

P

W

R

C

-

E

E

A

P

V

L

L

L

A

A

T

L

L

I

R

D

K

R

A

Y

V

L

E

N

R

A

R

P

L

L

V

E

S

D

D

L

V

A

P

P

-

R

-

F

-

D

-

T

V

V

G

G

V

I

L
x
P

L
|
L

S
|
S

G
|
G

L
|
L

D
|
D

S
|
S

S

S

I

L

V

V

V

T

A

A

L

L

L

A

A

S

E

R

A

H

G

F

Q

K

R

K

G

-

L

L

K

N

T

T

F

Y

S

S

I
|
I

G

-

F

-

E

-

S

-

V

-

G

G

G

T

E

E

A

L

G

S

D

N

E

E

F

F

T

E

Y

F

S

S

D

Q

I

Q

I

V

A

A

E

D

R

A

F

L

A

G

T

T

D

-

H

H

R

Q

I

M

R

K

I

I

D

L

S

S

A

E

R

T

A

E

L

V

P

-

A

-

L

I

H

N

G

G

A

I

V

I

A

E

A

S

M

I

S

Y

-

Y

-

N

-

E

-

I

-

F

E

D

P

G

M

L

V

S

S

A

H

E

D

I

A

Q

V

S

G

G

F

L

Y

F

L

N

L

V

S

Y

E

R

E

Q

V

A

S

Q

K

G

H

Q

V

V

K

S

V

C

V

M

Q

L

S

T

G
|
G

Q
x
Y

G
|
G

A
x
S

D
|
D

E

L

I

L

F

F

G

G

Y

I

H

-

W

-

Y

L

P

K

P

P

M

G

M

A

Q

Q

S

-

T

-

D

-

A

-

V

-

A

-

D

-

Y

-

A

-

R

-

V

-

F

-

F

Y

D

D

R

N

D

P

H

N

A

Q

E

L

M

L

R

A

E

E

A

-

L

-

S

-

P

-

S

-

L

-

V

-

D

-

G

-

D
x
Q

V

V

S

Y

R
|
R

A

T

F

R

V

W

E

T

A

G

H
x
E

F

F

A

A

R

T

A

H

G

G

A

A

P

S

R

-

P

-

I

-

D

-

K

-

A

-

L

-

R

-

I

-

D

-

T

-

E

-

I

-

M

-

L

-

V

-

D

-

P

-

V

-

K

-

R

-

V

-

D

-

N

-

M

-

T

-

M

-

A

C

W

Y

G

G

L

I

E

D

A

I

R

R

V

H

P

P

F

F

L

W

D

S

H

H

D

S

L

L

V

I

E

S

L

L

A

C

G

H

R

A

V

L

P

H

A

P

D

D

L

Y

K

K

I

I

R

F

D

D

G

N

G

E

-

V

K
|
K

F

N

I

I

L

L

K

R

Q

E

A

Y

G

A

R

D

-

S

-

L

R

Q

V

L

P

P

S

K

E

D

V

I

I

V

D

W

R

R

P

K

K
|
K

244:251 (vs. 266:273, 88% identical) binding ATP
I270 (= I293) binding ATP
GYGSD 344:348 (≠ GQGAD 370:374) binding ATP
Y345 (≠ Q371) mutation to A: Loss of activity.; mutation to F: Reduces catalytic efficiency.
G346 (= G372) binding substrate
Q371 (≠ D420) binding substrate
R374 (= R423) binding substrate
E380 (≠ H429) mutation to A: Loss of activity.; mutation to D: Reduces catalytic efficiency.; mutation to Q: Reduces catalytic efficiency.
K421 (= K497) binding ATP
K443 (= K517) mutation K->A,M: Loss of activity.

Sites not aligning to the query:

444:446 binding ATP

1xfgA Glutaminase domain of glucosamine 6-phosphate synthase complexed with l-glu hydroxamate (see paper)
42% identity, 11% coverage: 40:104/590 of query aligns to 64:127/238 of 1xfgA

query
sites
1xfgA

Q

H

G

G

I

T

A

G

F

I

G

A

H

H

R

T

R
|
R

L
x
W

S

A

I
x
T

L

H

-

G

D

E

L

P

S

S

P

E

A

V

S

N

Q

A

Q
x
H

P

P

M

H

V

V

D

S

P

E

G

H

L

I

G

-

L

-

A

V

I

V

V

V

F

H

N
|
N

G
|
G

C

I

I

I

Y

E

N

N

F

H

R

E

S

P

L

L

R

R

E

E

L

L

R

K

A

A

K

R

G

G

Y

Y

A

T

F

F

F

V

S

S

E

E

G

T

D
|
D

T

T

E

E

V

V

I

I

active site: W74 (≠ L50), N98 (= N75), G99 (= G76)
binding glutamine hydroxamate: R73 (= R49), W74 (≠ L50), T76 (≠ I52), H86 (≠ Q61), N98 (= N75), G99 (= G76), D123 (= D100)

Sites not aligning to the query:

active site: 1, 26, 27
binding glutamine hydroxamate: 1

1xffA Glutaminase domain of glucosamine 6-phosphate synthase complexed with glutamate (see paper)
42% identity, 11% coverage: 40:104/590 of query aligns to 64:127/238 of 1xffA

query
sites
1xffA

Q

H

G

G

I

T

A

G

F

I

G

A

H

H

R

T

R
|
R

L
x
W

S

A

I
x
T

L

H

-

G

D

E

L

P

S

S

P

E

A

V

S

N

Q

A

Q
x
H

P

P

M

H

V

V

D

S

P

E

G

H

L

I

G

-

L

-

A

V

I

V

V

V

F

H

N
|
N

G
|
G

C

I

I

I

Y

E

N

N

F

H

R

E

S

P

L

L

R

R

E

E

L

L

R

K

A

A

K

R

G

G

Y

Y

A

T

F

F

F

V

S

S

E

E

G

T

D
|
D

T

T

E

E

V

V

I

I

active site: W74 (≠ L50), N98 (= N75), G99 (= G76)
binding glutamic acid: R73 (= R49), W74 (≠ L50), T76 (≠ I52), H86 (≠ Q61), N98 (= N75), G99 (= G76), D123 (= D100)

Sites not aligning to the query:

active site: 1, 26, 27
binding glutamic acid: 1

2j6hA E. Coli glucosamine-6-p synthase in complex with glucose-6p and 5-oxo- l-norleucine (see paper)
42% identity, 11% coverage: 40:104/590 of query aligns to 64:127/608 of 2j6hA

query
sites
2j6hA

Q

H

G

G

I

T

A

G

F

I

G

A

H

H

R

T

R
|
R

L
x
W

S

A

I
x
T

L

H

-

G

D

E

L

P

S

S

P

E

A

V

S

N

Q

A

Q
x
H

P

P

M

H

V

V

D

S

P

E

G

H

L

I

G

-

L

-

A

V

I

V

V

V

F

H

N
|
N

G
|
G

C

I

I

I

Y

E

N

N

F

H

R

E

S

P

L

L

R

R

E

E

L

L

R

K

A

A

K

R

G

G

Y

Y

A

T

F

F

F

V

S

S

E

E

G

T

D
|
D

T

T

E

E

V

V

I

I

active site: W74 (≠ L50), N98 (= N75), G99 (= G76)
binding 5-oxo-l-norleucine: R73 (= R49), W74 (≠ L50), T76 (≠ I52), H86 (≠ Q61), N98 (= N75), G99 (= G76), D123 (= D100)

Sites not aligning to the query:

active site: 1, 26, 27, 248, 481, 485, 488, 504, 603
binding glucose-6-phosphate: 302, 347, 348, 349, 352, 399, 401, 488
binding 5-oxo-l-norleucine: 1

4amvA E.Coli glucosamine-6p synthase in complex with fructose-6p (see paper)
42% identity, 11% coverage: 40:104/590 of query aligns to 64:127/608 of 4amvA

query
sites
4amvA

Q

H

G

G

I

T

A

G

F

I

G

A

H

H

R

T

R

R

L
x
W

S

A

I

T

L

H

-

G

D

E

L

P

S

S

P

E

A

V

S

N

Q

A

Q

H

P

P

M

H

V

V

D

S

P

E

G

H

L

I

G

-

L

-

A

V

I

V

V

V

F

H

N
|
N

G
|
G

C

I

I

I

Y

E

N

N

F

H

R

E

S

P

L

L

R

R

E

E

L

L

R

K

A

A

K

R

G

G

Y

Y

A

T

F

F

F

V

S

S

E

E

G

T

D

D

T

T

E

E

V

V

I

I

active site: W74 (≠ L50), N98 (= N75), G99 (= G76)

Sites not aligning to the query:

active site: 1, 26, 27, 248, 481, 485, 488, 504, 603
binding fructose -6-phosphate: 301, 302, 303, 347, 348, 349, 352, 401, 485, 488

1jxaA Glucosamine 6-phosphate synthase with glucose 6-phosphate (see paper)
42% identity, 11% coverage: 40:104/590 of query aligns to 64:127/608 of 1jxaA

query
sites
1jxaA

Q

H

G

G

I

T

A

G

F

I

G

A

H

H

R

T

R

R

L
x
W

S

A

I

T

L

H

-

G

D

E

L

P

S

S

P

E

A

V

S

N

Q

A

Q

H

P

P

M

H

V

V

D

S

P

E

G

H

L

I

G

-

L

-

A

V

I

V

V

V

F

H

N
|
N

G
|
G

C

I

I

I

Y

E

N

N

F

H

R

E

S

P

L

L

R

R

E

E

L

L

R

K

A

A

K

R

G

G

Y

Y

A

T

F

F

F

V

S

S

E

E

G

T

D

D

T

T

E

E

V

V

I

I

active site: W74 (≠ L50), N98 (= N75), G99 (= G76)

Sites not aligning to the query:

active site: 1, 26, 27, 248, 481, 485, 488, 504, 603
binding glucose-6-phosphate: 302, 303, 347, 348, 349, 352, 401, 485, 488

Query Sequence

>WP_015926901.1 NCBI__GCF_000022085.1:WP_015926901.1
MCGICGEIRLDGAPPDAAAVAAMAVPLRARGPDAGGSFAQGGIAFGHRRLSILDLSPASQ
QPMVDPGLGLAIVFNGCIYNFRSLREELRAKGYAFFSEGDTEVILKAYHAWGPRCVERFY
GMFAFVLWERDSGRVVIARDRLGIKPLYYAESGSRLRFASSLPALLAAGDLDRSIDPAAL
HHYMSFHAVVPAPLTILKGVRKVPPATIITIEPDGRRRQETYWEAVVTERPDDRHMTESD
WCEAVLATLRKAVERRLVSDVPVGVLLSGGLDSSIVVALLAEAGQRGLKTFSIGFESVGG
EAGDEFTYSDIIAERFATDHHRIRIDSARALPALHGAVAAMSEPMVSHDAVGFYLLSEEV
SKHVKVVQSGQGADEIFGGYHWYPPMMQSTDAVADYARVFFDRDHAEMREALSPSLVDGD
VSRAFVEAHFARAGAPRPIDKALRIDTEIMLVDDPVKRVDNMTMAWGLEARVPFLDHDLV
ELAGRVPADLKIRDGGKFILKQAGRRVVPSEVIDRPKGYFPVPALKYLRGPFLDLVRDVL
NEPVARERGLFNRAYVDRLLAEPEAHMTPLRGSKLWQVALLEFWLQTHAI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory