SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_015927721.1 NCBI__GCF_000022085.1:WP_015927721.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

3tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2-amino- 6-oxopimelate and coenzyme a (see paper)
65% identity, 98% coverage: 6:280/281 of query aligns to 4:271/274 of 3tdtA

query
sites
3tdtA

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active site: D141 (= D145), G166 (= G170), E189 (= E193)
binding 2-amino-6-oxopimelic acid: R104 (= R108), M124 (= M128), M139 (= M143), D141 (= D145)
binding coenzyme a: G185 (= G189), A186 (= A190), S203 (= S207), M204 (= M208), R217 (= R221), V234 (= V238), K254 (= K263), K259 (≠ G268), T260 (= T269), K263 (= K272)

2tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2- aminopimelate and coenzyme a (see paper)
65% identity, 98% coverage: 6:280/281 of query aligns to 4:271/274 of 2tdtA

query
sites
2tdtA

L

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active site: D141 (= D145), G166 (= G170), E189 (= E193)
binding coenzyme a: S159 (= S163), F183 (= F187), G185 (= G189), S203 (= S207), M204 (= M208), R217 (= R221), K254 (= K263), K259 (≠ G268), T260 (= T269), K263 (= K272)
binding (2S)-2-aminoheptanedioic acid: R104 (= R108), M124 (= M128), M139 (= M143), D141 (= D145)

1kgtA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with pimelate and succinyl-coa (see paper)
65% identity, 98% coverage: 6:280/281 of query aligns to 4:271/274 of 1kgtA

query
sites
1kgtA

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active site: D141 (= D145), G166 (= G170), E189 (= E193)
binding pimelic acid: R104 (= R108), M124 (= M128), M139 (= M143)
binding succinyl-coenzyme a: D141 (= D145), S159 (= S163), A186 (= A190), S203 (= S207), R217 (= R221), K259 (≠ G268), T260 (= T269), K263 (= K272)

1kgqA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with l-2-aminopimelate and succinamide-coa (see paper)
65% identity, 98% coverage: 6:280/281 of query aligns to 4:271/274 of 1kgqA

query
sites
1kgqA

L

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active site: D141 (= D145), G166 (= G170), E189 (= E193)
binding (2S)-2-aminoheptanedioic acid: R104 (= R108), M124 (= M128), M139 (= M143), D141 (= D145)
binding succinamide-coa: F183 (= F187), G185 (= G189), S203 (= S207), M204 (= M208), R217 (= R221), V234 (= V238), K254 (= K263), K259 (≠ G268), T260 (= T269), K263 (= K272)

3r8yA Structure of the bacillus anthracis tetrahydropicolinate succinyltransferase
50% identity, 32% coverage: 108:198/281 of query aligns to 77:168/203 of 3r8yA

query
sites
3r8yA

R

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I

L

A

A

G
|
G

V

V

L

I

E

E

P

P

L

P

Q

S

A

A

N

K

P

P

V

V

I

I

I

V

E

E

D

D

N

D

C

V

F

V

I

I

G

G

A

A

R

N

A

V

E
x
V

V

V

A

L

E

E

G

G

V

V

active site: D115 (= D145), G140 (= G170), V163 (≠ E193)
binding calcium ion: G121 (= G151), G122 (≠ S152)

Query Sequence

>WP_015927721.1 NCBI__GCF_000022085.1:WP_015927721.1
MSHADLARTIEAAWEDRASVGPTTQGTVREAVEAALALLDSGQARVAEKSGSADWQVNQW
LKKAVLLSFRLNDMSVIPGGPGGAAWWDKVPSKFEGWDADRFRAAGFRAVPGAVVRRGSY
IAPGAVLMPSFVNLGAYVGEGTMVDTWATIGSCAQVGKNCHISGGAGIAGVLEPLQANPV
IIEDNCFIGARAEVAEGVIVGEGSVLSMGVYIGASTKIIDRATGEVMYGRVPPYSVVVSG
TQPGKPLPDGTPGPSLYCAVIVKRVDAGTRAKTGINELLRD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory