SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_015927792.1 NCBI__GCF_000022085.1:WP_015927792.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ij6A Crystal structure of a novel-type phosphoserine phosphatase mutant (h9a) from hydrogenobacter thermophilus tk-6 in complex with l-phosphoserine (see paper)
32% identity, 82% coverage: 22:185/199 of query aligns to 18:186/207 of 4ij6A

query
sites
4ij6A

G

G

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Y

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x
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active site: R58 (= R59), E82 (= E83), H150 (= H149)
binding phosphoserine: Q21 (≠ P25), R58 (= R59), E82 (= E83), H85 (≠ F86), H150 (= H149), T151 (≠ A150)

Sites not aligning to the query:

active site: 8, 9, 15
binding phosphoserine: 8

6m1xC Crystal structure of phosphoserine phosphatase in complex with 3- phosphoglyceric acid from entamoeba histolytica (see paper)
28% identity, 91% coverage: 3:183/199 of query aligns to 2:180/196 of 6m1xC

query
sites
6m1xC

T

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binding 3-phosphoglyceric acid: R8 (= R9), H9 (= H10), Q21 (≠ P25), R57 (= R59), E79 (= E83), H146 (= H149), G147 (≠ A150)

1h2fA Bacillus stearothermophilus phoe (previously known as yhfr) in complex with trivanadate (see paper)
31% identity, 78% coverage: 3:158/199 of query aligns to 2:159/207 of 1h2fA

query
sites
1h2fA

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active site: R8 (= R9), H9 (= H10), N15 (≠ T19), R58 (= R59), E82 (= E83), H150 (= H149)
binding phosphate ion: T2 (= T3), T3 (= T4), G142 (≠ E141), E143 (≠ A142)
binding trivanadate: R8 (= R9), H9 (= H10), N15 (≠ T19), Q21 (≠ P25), R58 (= R59), E82 (= E83), H150 (= H149), G151 (≠ A150), V152 (≠ D151)

Sites not aligning to the query:

binding phosphate ion: 1

1h2eA Bacillus stearothermophilus phoe (previously known as yhfr) in complex with phosphate (see paper)
31% identity, 78% coverage: 3:158/199 of query aligns to 2:159/207 of 1h2eA

query
sites
1h2eA

T

T

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active site: R8 (= R9), H9 (= H10), N15 (≠ T19), R58 (= R59), E82 (= E83), H150 (= H149)
binding phosphate ion: R8 (= R9), H9 (= H10), R58 (= R59), E82 (= E83), H150 (= H149)

5zr2C Crystal structure of phosphoserine phosphatase mutant (h9a) from entamoeba histolytica in complex with phosphoserine (see paper)
27% identity, 85% coverage: 14:183/199 of query aligns to 10:180/198 of 5zr2C

query
sites
5zr2C

G

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binding phosphoserine: Q21 (≠ P25), G22 (= G26), R57 (= R59), E79 (= E83), H146 (= H149), G147 (≠ A150)

Sites not aligning to the query:

binding phosphoserine: 8

1k6mA Crystal structure of human liver 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase (see paper)
27% identity, 98% coverage: 4:199/199 of query aligns to 214:404/432 of 1k6mA

query
sites
1k6mA

T

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A

active site: R219 (= R9), H220 (= H10), N226 (≠ T19), R269 (= R59), E289 (= E83), H354 (= H149)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 13, 14, 15, 16, 17, 18, 92, 120, 131, 134, 135, 136, 184, 210

1tipA The bisphosphatase domain of the bifunctional rat liver 6- phosphofructo-2-kinase/fructose-2,6-bisphosphatase (see paper)
27% identity, 95% coverage: 4:193/199 of query aligns to 3:187/191 of 1tipA

query
sites
1tipA

T

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H

A

Y

D

P

P

E

R

E

W

F

T

A

L

L

W
x
R

N

D

T

Q

A

D

R
x
K

H

Y

V

R

T

Y

R

R

P

Y

P

P

G

K

G

G

E

E

T

S

I
x
Y

L

E

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

E

A

R

S

-

H

-

G

-

E

Q

A

E

R

N

I

V

A

L

L

V

V

I

S

C

H
|
H

A

Q

D

A

L

V

I

M

K
x
R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

S

P

S

D

D

A

E

L

L

G

P

R

Y

F

L

E

K

V

C

S

P

P

L

A

H

S

T

I

V

S

L

A

K

V

L

V

T

L

P

G

V

E

A

W

Y

G

G

A

C

K

R

V

V

E

E

S

S

I

I

active site: R8 (= R9), H9 (= H10), N15 (≠ T19), R58 (= R59), E78 (= E83), H143 (= H149)
binding 6-O-phosphono-beta-D-fructofuranose: I20 (≠ M24), Y89 (≠ F94), R103 (≠ W106), K107 (≠ R110), Y118 (≠ I121), R148 (≠ K154)

1c81A Michaelis complex of fructose-2,6-bisphosphatase
27% identity, 95% coverage: 4:193/199 of query aligns to 3:187/191 of 1c81A

query
sites
1c81A

T

S

F

I

F

Y

L

L

V

C

R
|
R

H
|
H

A

-

H

-

G

G

H

E

L

S

D

E

R

L

T
x
N

L

L

C

R

G

G

R

R

M
x
I

P

G

G

G

I

D

R

S

-

G

L

L

G

S

E

A

E

R

G

G

R

K

A

Q

Q

Y

A

A

R

Y

A

A

L

L

A

A

A

N

R

F

L

I

K

R

S

S

E

Q

R

G

L

I

D

S

A

S

-

L

-

K

V

V

Y

W

A

T

S

S

P

H

L

M

E

K

R
|
R

A

T

Q

I

E

Q

T

T

G

A

E

E

P

-

I

-

A

-

A

A

A

L

G

G

V

V

P

P

M

Y

R

E

V

Q

L

W

P

K

A

A

L

L

N

N

E
|
E

I

I

D

D

F

A

G

G

A

V

W

C

S

E

G

E

C

M

S

T

F

Y

E

E

L

I

-

Q

-

E

H

H

A

Y

D

P

P

E

R

E

W

F

T

A

L

L

W

R

N

D

T

Q

A

D

R

K

H

Y

V

R

T

Y

R

R

P

Y

P

P

G

K

G

G

E

E

T

S

I

Y

L

E

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

E

A

R

S

-

H

-

G

-

E

Q

A

E

R

N

I

V

A

L

L

V

V

I

S

C

H
|
H

A
x
Q

D

A

L

V

I

M

K
x
R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

S

P

S

D

D

A

E

L

L

G

P

R

Y

F

L

E

K

V

C

S

P

P

L

A

H

S

T

I

V

S

L

A

K

V

L

V

T

L

P

G

V

E

A

W

Y

G

G

A

C

K

R

V

V

E

E

S

S

I

I

active site: R8 (= R9), H9 (= H10), N15 (≠ T19), R58 (= R59), E78 (= E83), H143 (= H149)
binding 2,5-anhydro-1-deoxy-1-phosphono-6-O-phosphono-D-glucitol: R8 (= R9), H9 (= H10), N15 (≠ T19), I20 (≠ M24), R58 (= R59), E78 (= E83), H143 (= H149), Q144 (≠ A150), R148 (≠ K154)

1c80A Regulatory complex of fructose-2,6-bisphosphatase
27% identity, 95% coverage: 4:193/199 of query aligns to 3:187/191 of 1c80A

query
sites
1c80A

T

S

F

I

F

Y

L

L

V

C

R
|
R

H
|
H

A

-

H

-

G

G

H

E

L

S

D

E

R

L

T
x
N

L

L

C

R

G

G

R

R

M
x
I

P

G

G

G

I

D

R

S

-

G

L

L

G

S

E

A

E

R

G

G

R

K

A

Q

Q

Y

A

A

R

Y

A

A

L

L

A

A

A

N

R

F

L

I

K

R

S

S

E

Q

R

G

L

I

D

S

A

S

-

L

-

K

V

V

Y

W

A

T

S

S

P

H

L

M

E

K

R
|
R

A

T

Q

I

E

Q

T

T

G

A

E

E

P

-

I

-

A

-

A

A

A

L

G

G

V

V

P

P

M

Y

R

E

V

Q

L

W

P

K

A

A

L

L

N

N

E
|
E

I
|
I

D

D

F

A

G

G

A

V

W

C

S

E

G

E

C

M

S

T

F
x
Y

E

E

L

I

-

Q

-

E

H

H

A

Y

D

P

P

E

R

E

W
x
F

T

A

L

L

W
x
R

N

D

T

Q

A

D

R

K

H

Y

V

R

T

Y

R

R

P
x
Y

P

P

G

K

G

G

E

E

T

S

I
x
Y

L

E

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

E

A

R

S

-

H

-

G

-

E

Q

A

E

R

N

I

V

A

L

L

V

V

I

S

C

H
|
H

A
x
Q

D
x
A

L

V

I

M

K
x
R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

S

P

S

D

D

A

E

L

L

G

P

R

Y

F

L

E

K

V

C

S

P

P

L

A

H

S

T

I

V

S

L

A

K

V

L

V

T

L

P

G

V

E

A

W

Y

G

G

A

C

K

R

V

V

E

E

S

S

I

I

active site: R8 (= R9), H9 (= H10), N15 (≠ T19), R58 (= R59), E78 (= E83), H143 (= H149)
binding guanosine-5'-triphosphate: I20 (≠ M24), E78 (= E83), I79 (= I84), Y89 (≠ F94), F100 (≠ W103), R103 (≠ W106), Y112 (≠ P115), Y118 (≠ I121), Q144 (≠ A150), A145 (≠ D151), R148 (≠ K154)

1c7zA Regulatory complex of fructose-2,6-bisphosphatase
27% identity, 95% coverage: 4:193/199 of query aligns to 3:187/191 of 1c7zA

query
sites
1c7zA

T

S

F

I

F

Y

L

L

V

C

R
|
R

H
|
H

A

-

H

-

G

G

H

E

L

S

D

E

R

L

T
x
N

L

L

C

R

G

G

R

R

M

I

P

G

G

G

I

D

R

S

-

G

L

L

G

S

E

A

E

R

G

G

R

K

A

Q

Q

Y

A

A

R

Y

A

A

L

L

A

A

A

N

R

F

L

I

K

R

S

S

E

Q

R

G

L

I

D

S

A

S

-

L

-

K

V

V

Y

W

A

T

S

S

P

H

L

M

E

K

R
|
R

A

T

Q

I

E

Q

T

T

G

A

E

E

P

-

I

-

A

-

A

A

A

L

G

G

V

V

P

P

M

Y

R

E

V

Q

L

W

P

K

A

A

L

L

N

N

E
|
E

I

I

D

D

F

A

G

G

A

V

W

C

S

E

G

E

C

M

S

T

F
x
Y

E

E

L

I

-

Q

-

E

H

H

A

Y

D

P

P

E

R

E

W

F

T

A

L

L

W
x
R

N

D

T

Q

A

D

R
x
K

H

Y

V

R

T

Y

R

R

P

Y

P

P

G

K

G

G

E

E

T

S

I
x
Y

L

E

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

E

A

R

S

-

H

-

G

-

E

Q

A

E

R

N

I

V

A

L

L

V

V

I

S

C

H
|
H

A
x
Q

D
x
A

L

V

I

M

K
x
R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

S

P

S

D

D

A

E

L

L

G

P

R

Y

F

L

E

K

V

C

S

P

P

L

A

H

S

T

I

V

S

L

A

K

V

L

V

T

L

P

G

V

E

A

W

Y

G

G

A

C

K

R

V

V

E

E

S

S

I

I

active site: R8 (= R9), H9 (= H10), N15 (≠ T19), R58 (= R59), E78 (= E83), H143 (= H149)
binding glyceraldehyde-3-phosphate: Y89 (≠ F94), R103 (≠ W106), K107 (≠ R110), Y118 (≠ I121), Q144 (≠ A150), A145 (≠ D151), R148 (≠ K154)

1fbtA The bisphosphatase domain of the bifunctional rat liver 6- phosphofructo-2-kinase/fructose-2,6-bisphosphatase (see paper)
27% identity, 95% coverage: 4:193/199 of query aligns to 2:186/190 of 1fbtA

query
sites
1fbtA

T

S

F

I

F

Y

L

L

V

C

R
|
R

H
|
H

A

-

H

-

G

G

H

E

L

S

D

E

R

L

T
x
N

L

L

C

R

G

G

R

R

M

I

P

G

G

G

I

D

R

S

-

G

L

L

G

S

E

A

E

R

G

G

R

K

A

Q

Q

Y

A

A

R

Y

A

A

L

L

A

A

A

N

R

F

L

I

K

R

S

S

E

Q

R

G

L

I

D

S

A

S

-

L

-

K

V

V

Y

W

A

T

S

S

P

H

L

M

E

K

R
|
R

A

T

Q

I

E

Q

T

T

G

A

E

E

P

-

I

-

A

-

A

A

A

L

G

G

V

V

P

P

M

Y

R

E

V

Q

L

W

P

K

A

A

L

L

N

N

E
|
E

I

I

D

D

F

A

G

G

A

V

W

C

S

E

G

E

C

M

S

T

F

Y

E

E

L

I

-

Q

-

E

H

H

A

Y

D

P

P

E

R

E

W

F

T

A

L

L

W

R

N

D

T

Q

A

D

R

K

H

Y

V

R

T

Y

R

R

P

Y

P

P

G

K

G

G

E

E

T

S

I

Y

L

E

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

E

A

R

S

-

H

-

G

-

E

Q

A

E

R

N

I

V

A

L

L

V

V

I

S

C

H
|
H

A
x
Q

D

A

L

V

I

M

K

R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

S

P

S

D

D

A

E

L

L

G

P

R

Y

F

L

E

K

V

C

S

P

P

L

A

H

S

T

I

V

S

L

A

K

V

L

V

T

L

P

G

V

E

A

W

Y

G

G

A

C

K

R

V

V

E

E

S

S

I

I

active site: R7 (= R9), H8 (= H10), N14 (≠ T19), R57 (= R59), E77 (= E83), H142 (= H149)
binding phosphate ion: R7 (= R9), H8 (= H10), R57 (= R59), E77 (= E83), H142 (= H149), Q143 (≠ A150)

5htkA Human heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (pfkfb2) (see paper)
27% identity, 98% coverage: 4:199/199 of query aligns to 221:411/425 of 5htkA

query
sites
5htkA

T

T

F

I

F

Y

L

L

V

C

R
|
R

H
|
H

A

-

H

-

G

G

H

E

L

S

D

E

R

F

T
x
N

L

L

C

L

G

G

R

K

M
x
I

P
x
G

G

G

I

D

R

S

-

G

L

L

G

S

E

V

E

R

G

G

R

K

A

Q

Q

F

A

A

R

Q

A

A

L

L

A

R

A

K

R

F

L

L

K

E

S

E

E

Q

R

E

L

I

D

T

-

D

-

L

A

K

V

V

Y

W

A

T

S

S

P

Q

L

L

E

K

R
|
R

A

T

Q

I

E

Q

T

T

G

A

E

E

P

S

I

L

A

-

G

G

V

V

P

P

M

Y

R

E

V

Q

L

W

P

K

A

I

L

L

N

N

E
|
E

I

I

D

D

F

A

G

G

A

V

W

C

S

E

G

E

C

M

S

T

F
x
Y

E

A

E

E

L

I

H

E

A

K

D

R

-

Y

-

P

P

E

R

E

W

F

T

A

L

L

W
x
R

N

D

T

Q

A

E

R
x
K

H

Y

V

L

T

Y

R

R

P

Y

P

P

G

G

E

E

T

S

I
x
Y

L

Q

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

-

A

-

S

-

H

E

G

R

E

Q

A

G

R

N

I

V

A

L

L

V

V

I

S

S

H
|
H

A
x
Q

D

A

L

V

I

M

K
x
R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

G

P

A

D

D

A

E

L

L

G

P

R

Y

F

L

E

R

V

C

S

P

P

L

A

H

S

T

I

I

S

F

A

K

V

L

V

T

L

P

G

V

E
x
A

W
x
Y

G

G

A

C

K

K

V

V

E

E

S

T

I

I

N

K

E

L

R

N

V

V

S

E

A

A

active site: R226 (= R9), H227 (= H10), N233 (≠ T19), R276 (= R59), E296 (= E83), H361 (= H149)
binding adenosine-5'-triphosphate: A397 (≠ E185), Y398 (≠ W186)
binding 6-O-phosphono-beta-D-fructofuranose: H227 (= H10), I238 (≠ M24), G239 (≠ P25), E296 (= E83), Y307 (≠ F94), R321 (≠ W106), K325 (≠ R110), Y336 (≠ I121), Q362 (≠ A150), R366 (≠ K154)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 17, 18, 19, 20, 21, 22, 140, 144, 145, 146
binding citrate anion: 48, 61, 72, 99, 100, 167

1bifA 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase bifunctional enzyme complexed with atp-g-s and phosphate (see paper)
26% identity, 98% coverage: 4:199/199 of query aligns to 214:404/432 of 1bifA

query
sites
1bifA

T

S

F

I

F

Y

L

L

V

C

R
|
R

H
|
H

A

-

H

-

G

G

H

E

L

S

D

E

R

L

T
x
N

L

L

C

K

G

G

R

R

M

I

-

G

-

D

P

P

G

G

I

-

R

-

L

L

G

S

E

P

E

R

G

G

R

R

A

E

Q

F

A

S

R

K

A

H

L

L

A

A

A

Q

R

F

L

I

K

S

S

D

E

Q

R

N

L

I

D

K

-

D

-

L

A

K

V

V

Y

F

A

T

S

S

P

Q

L

M

E

K

R
|
R

A

T

Q

I

E

Q

T

T

G

A

E

E

P

-

I

-

A

-

A

A

A

L

G

S

V

V

P

P

M

Y

R

E

V

Q

L

F

P

K

A

V

L

L

N

N

E
|
E

I

I

D

D

F

A

G

G

A

V

W

C

S

E

G

E

C

M

S

T

F
x
Y

E

E

L

I

-

Q

-

D

H

H

A

Y

D

P

P

L

R

E

W

F

T

A

L

L

W
x
R

N

D

T

Q

A

D

R
x
K

H

Y

V

R

T

Y

R

R

P

Y

P

P

G

K

G

G

E

E

T

S

I
x
Y

L

E

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

E

A

R

S

Q

H

E

G

N

E

-

A

-

R

-

I

V

A

L

L

V

V

I

S

C

H
|
H

A

Q

D

A

L

V

I

M

K
x
R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

A

P

A

D

E

A

E

L

L

G

P

R

Y

F

L

E

K

V

C

S

P

P

L

A

H

S

T

I

V

S

L

A

K

V

L

V

T

L

P

G

V

E

A

W
x
Y

G

G

A

C

K

K

V

V

E

E

S

S

I

I

N

F

E

L

R

N

V

V

S

A

A

A

active site: R219 (= R9), H220 (= H10), N226 (≠ T19), R269 (= R59), E289 (= E83), H354 (= H149)
binding phosphothiophosphoric acid-adenylate ester: Y391 (≠ W186)
binding phosphate ion: R219 (= R9), H220 (= H10), R269 (= R59), E289 (= E83), Y300 (≠ F94), R314 (≠ W106), K318 (≠ R110), Y329 (≠ I121), H354 (= H149), R359 (≠ K154)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 11, 12, 13, 14, 15, 16, 17, 131, 134, 136, 184, 210

P25114 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4; 6PF-2-K/Fru-2,6-P2ase 4; PFK/FBPase 4; 6PF-2-K/Fru-2,6-P2ase testis-type isozyme; EC 2.7.1.105; EC 3.1.3.46 from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 98% coverage: 4:199/199 of query aligns to 251:441/469 of P25114

query
sites
P25114

T

S

F

I

F

Y

L

L

V

C

R

R

H
|
H

A

-

H

-

G

G

H

E

L

S

D

E

R

L

T

N

L

L

C

K

G

G

R

R

M

I

-
x
G

-

G

-

D

P

P

G

G

I

-

R

-

L

L

G

S

E

P

E

R

G

G

R

R

A

E

Q

F

A

S

R

K

A

H

L

L

A

A

A

Q

R

F

L

I

K

S

S

D

E

Q

R

N

L

I

D

K

-

D

-

L

A

K

V

V

Y

W

A

T

S

S

P

Q

L

M

E

K

R

R

A

T

Q

I

E

Q

T

T

G

A

E

E

P

-

I

-

A

-

A

A

A

L

G

S

V

V

P

P

M

Y

R

E

V

Q

L

W

P

K

A

V

L

L

N

N

E
|
E

I

I

D

D

F

A

G

G

A

V

W

C

S

E

G

E

C

M

S

T

F
x
Y

E

E

L

I

-

Q

-

D

H

H

A

Y

D

P

P

L

R

E

W

F

T

A

L

L

W
x
R

N

D

T

Q

A

D

R
x
K

H

Y

V

R

T

Y

R

R

P

Y

P

P

G

K

G

G

E

E

T

S

I
x
Y

L

E

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

E

A

R

S

Q

H

E

G

N

E

-

A

-

R

-

I

V

A

L

L

V

V

I

S

C

H

H

A
x
Q

D

A

L

V

I

M

K
x
R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

A

P

A

D

E

A

E

L

L

G

P

R

Y

F

L

E

K

V

C

S

P

P

L

A

H

S

T

I

V

S

L

A

K

V

L

V

T

L

P

G

V

E

A

W
x
Y

G

G

A

C

K

K

V

V

E

E

S

S

I

I

N

F

E

L

R

N

V

V

S

A

A

A

H257 (= H10) active site, Tele-phosphohistidine intermediate
G269 (vs. gap) binding
E326 (= E83) active site, Proton donor/acceptor
Y337 (≠ F94) binding
R351 (≠ W106) binding
K355 (≠ R110) binding
Y366 (≠ I121) binding
Q392 (≠ A150) binding
R396 (≠ K154) binding
Y428 (≠ W186) binding

Sites not aligning to the query:

46:54 binding
79 binding
131 binding
137 binding
168:173 binding
198 binding

2bifA 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase h256a mutant with f6p in phosphatase active site (see paper)
26% identity, 93% coverage: 14:199/199 of query aligns to 221:404/432 of 2bifA

query
sites
2bifA

G

G

H

E

L

S

D

E

R

L

T
x
N

L

L

C

K

G

G

R

R

M
x
I

-
x
G

-

G

-

D

P

P

G

G

I

-

R

-

L

L

G

S

E

P

E

R

G

G

R

R

A

E

Q

F

A

S

R

K

A

H

L

L

A

A

A

Q

R

F

L

I

K

S

S

D

E

Q

R

N

L

I

D

K

-

D

-

L

A

K

V

V

Y

F

A

T

S

S

P

Q

L

M

E

K

R
|
R

A

T

Q

I

E

Q

T

T

G

A

E

E

P

-

I

-

A

-

A

A

A

L

G

S

V

V

P

P

M

Y

R

E

V

Q

L

F

P

K

A

V

L

L

N

N

E
|
E

I

I

D

D

F

A

G

G

A

V

W

C

S

E

G

E

C

M

S

T

F
x
Y

E

E

L

I

-

Q

-

D

H

H

A

Y

D

P

P

L

R

E

W

F

T

A

L

L

W
x
R

N

D

T

Q

A

D

R
x
K

H

Y

V

R

T

Y

R

R

P

Y

P

P

G

K

G

G

E

E

T

S

I
x
Y

L

E

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

E

A

R

S

Q

H

E

G

N

E

-

A

-

R

-

I

V

A

L

L

V

V

I

S

C

H
|
H

A
x
Q

D

A

L

V

I

M

K
x
R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

A

P

A

D

E

A

E

L

L

G

P

R

Y

F

L

E

K

V

C

S

P

P

L

A

H

S

T

I

V

S

L

A

K

V

L

V

T

L

P

G

V

E

A

W
x
Y

G

G

A

C

K

K

V

V

E

E

S

S

I

I

N

F

E

L

R

N

V

V

S

A

A

A

active site: N226 (≠ T19), R269 (= R59), E289 (= E83), H354 (= H149)
binding phosphoaminophosphonic acid-adenylate ester: Y391 (≠ W186)
binding 6-O-phosphono-beta-D-fructofuranose: I231 (≠ M24), G232 (vs. gap), E289 (= E83), Y300 (≠ F94), R314 (≠ W106), K318 (≠ R110), Y329 (≠ I121), Q355 (≠ A150), R359 (≠ K154)
binding phosphate ion: N226 (≠ T19), R269 (= R59), H354 (= H149)

Sites not aligning to the query:

active site: 219, 220
binding phosphoaminophosphonic acid-adenylate ester: 11, 12, 13, 14, 15, 16, 17, 131, 184
binding 6-O-phosphono-beta-D-fructofuranose: 407
binding magnesium ion: 15, 16, 92
binding phosphate ion: 219

6ibxA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 5 (see paper)
25% identity, 95% coverage: 4:193/199 of query aligns to 230:414/429 of 6ibxA

query
sites
6ibxA

T

T

F

I

F

Y

L

L

V

C

R

R

H

H

A

G

A

E

H

N

G

E

H

H

L

-

D

-

R

-

T

N

L

L

C

Q

G

G

R

R

M

I

P
x
G

G

G

I

D

R

S

-

G

L

L

G

S

E

S

E

R

G

G

R

K

A

K

Q

F

A

A

R

S

A

A

L

L

A

S

A

K

R

F

L

V

K

E

S

E

E

Q

R

N

L

L

D

K

A

D

-

L

-

R

V

V

Y

W

A

T

S

S

P

Q

L

L

E

K

R

S

A

T

Q

I

E

Q

T

T

G

A

E

E

P

-

I

-

A

-

A

A

A

L

G

R

V

L

P

P

M

Y

R

E

V

Q

L

W

P

K

A

A

L

L

N

N

E
|
E

I

I

D

D

F

A

G

G

A

V

W

C

S

E

G

E

C

L

S

T

F
x
Y

E

E

L

I

H

R

A

D

D

T

-

Y

-

P

P

E

R

E

W

Y

T

A

L

L

W
x
R

N

E

T

Q

A

D

R
x
K

H

Y

V

Y

T

Y

R

R

P

Y

P

P

G

T

G

G

E

E

T

S

I
x
Y

L

Q

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

E

A

R

S

Q

H

E

G

N

E

-

A

-

R

-

I

V

A

L

L

V

V

I

S

C

H

H

A
x
Q

D

A

L

V

I

L

K
x
R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

S

P

A

D

E

A

E

L

M

G

P

R

Y

F

L

E

K

V

C

S

P

P

L

A

H

S

T

I

V

S

L

A

K

V

L

V

T

L

P

G

V

E

A

W
x
Y

G

G

A

C

K

R

V

V

E

E

S

S

I

I

binding 6-O-phosphono-beta-D-fructofuranose: G248 (≠ P25), E305 (= E83), Y316 (≠ F94), R330 (≠ W106), K334 (≠ R110), Y345 (≠ I121), Q371 (≠ A150), R375 (≠ K154)
binding pyrophosphate 2-: Y407 (≠ W186)

Sites not aligning to the query:

binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-[(3~{S})-1-methylpiperidin-3-yl]pyridine-4-carboxamide: 28, 32, 33, 135, 137, 142, 146, 149, 150, 197, 224
binding pyrophosphate 2-: 27, 28, 29, 30, 31, 32, 151

6ibzA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 7 (see paper)
25% identity, 95% coverage: 4:193/199 of query aligns to 232:416/431 of 6ibzA

query
sites
6ibzA

T

T

F

I

F

Y

L

L

V

C

R

R

H

H

A

G

A

E

H

N

G

E

H

H

L

-

D

-

R

-

T

N

L

L

C

Q

G

G

R

R

M

I

P
x
G

G

G

I

D

R

S

-

G

L

L

G

S

E

S

E

R

G

G

R

K

A

K

Q

F

A

A

R

S

A

A

L

L

A

S

A

K

R

F

L

V

K

E

S

E

E

Q

R

N

L

L

D

K

A

D

-

L

-

R

V

V

Y

W

A

T

S

S

P

Q

L

L

E

K

R

S

A

T

Q

I

E

Q

T

T

G

A

E

E

P

-

I

-

A

-

A

A

A

L

G

R

V

L

P

P

M

Y

R

E

V

Q

L

W

P

K

A

A

L

L

N

N

E
|
E

I

I

D

D

F

A

G

G

A

V

W

C

S

E

G

E

C

L

S

T

F
x
Y

E

E

L

I

H

R

A

D

D

T

-

Y

-

P

P

E

R

E

W

Y

T

A

L

L

W
x
R

N

E

T

Q

A

D

R
x
K

H

Y

V

Y

T

Y

R

R

P

Y

P

P

G

T

G

G

E

E

T

S

I
x
Y

L

Q

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

E

A

R

S

Q

H

E

G

N

E

-

A

-

R

-

I

V

A

L

L

V

V

I

S

C

H

H

A
x
Q

D

A

L

V

I

L

K
x
R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

S

P

A

D

E

A

E

L

M

G

P

R

Y

F

L

E

K

V

C

S

P

P

L

A

H

S

T

I

V

S

L

A

K

V

L

V

T

L

P

G

V

E

A

W

Y

G

G

A

C

K

R

V

V

E

E

S

S

I

I

binding 6-O-phosphono-beta-D-fructofuranose: G250 (≠ P25), E307 (= E83), Y318 (≠ F94), R332 (≠ W106), K336 (≠ R110), Y347 (≠ I121), Q373 (≠ A150), R377 (≠ K154)

Sites not aligning to the query:

binding 2-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-(pyrimidin-5-ylmethyl)benzenesulfonamide: 30, 34, 35, 137, 139, 144, 148, 152, 199, 202, 226, 228
binding phosphate ion: 28, 29, 30, 31, 32, 33, 153

6hviA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 2 (see paper)
25% identity, 95% coverage: 4:193/199 of query aligns to 234:418/433 of 6hviA

query
sites
6hviA

T

T

F

I

F

Y

L

L

V

C

R
|
R

H
|
H

A

G

A

E

H

N

G

E

H

H

L

-

D

-

R

-

T
x
N

L

L

C

Q

G

G

R

R

M

I

P
x
G

G

G

I

D

R

S

-

G

L

L

G

S

E

S

E

R

G

G

R

K

A

K

Q

F

A

A

R

S

A

A

L

L

A

S

A

K

R

F

L

V

K

E

S

E

E

Q

R

N

L

L

D

K

A

D

-

L

-

R

V

V

Y

W

A

T

S

S

P

Q

L

L

E

K

R
x
S

A

T

Q

I

E

Q

T

T

G

A

E

E

P

-

I

-

A

-

A

A

A

L

G

R

V

L

P

P

M

Y

R

E

V

Q

L

W

P

K

A

A

L

L

N

N

E
|
E

I

I

D

D

F

A

G

G

A

V

W

C

S

E

G

E

C

L

S

T

F
x
Y

E

E

L

I

H

R

A

D

D

T

-

Y

-

P

P

E

R

E

W

Y

T

A

L

L

W
x
R

N

E

T

Q

A

D

R
x
K

H

Y

V

Y

T

Y

R

R

P

Y

P

P

G

T

G

G

E

E

T

S

I
x
Y

L

Q

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

E

A

R

S

Q

H

E

G

N

E

-

A

-

R

-

I

V

A

L

L

V

V

I

S

C

H
|
H

A
x
Q

D

A

L

V

I

L

K
x
R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

S

P

A

D

E

A

E

L

M

G

P

R

Y

F

L

E

K

V

C

S

P

P

L

A

H

S

T

I

V

S

L

A

K

V

L

V

T

L

P

G

V

E

A

W
x
Y

G

G

A

C

K

R

V

V

E

E

S

S

I

I

active site: R239 (= R9), H240 (= H10), N246 (≠ T19), S289 (≠ R59), E309 (= E83), H374 (= H149)
binding 6-O-phosphono-beta-D-fructofuranose: G252 (≠ P25), E309 (= E83), Y320 (≠ F94), R334 (≠ W106), K338 (≠ R110), Y349 (≠ I121), Q375 (≠ A150), R379 (≠ K154)
binding pyrophosphate 2-: Y411 (≠ W186)

Sites not aligning to the query:

binding 8-[3-(dimethylamino)phenyl]-~{N}-(4-methylsulfonylpyridin-3-yl)quinoxalin-6-amine: 32, 33, 36, 37, 139, 141, 146, 150, 153, 201, 204, 228, 230
binding pyrophosphate 2-: 31, 32, 33, 34, 35, 36, 155

6hvhA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 1 (see paper)
25% identity, 95% coverage: 4:193/199 of query aligns to 232:416/431 of 6hvhA

query
sites
6hvhA

T

T

F

I

F

Y

L

L

V

C

R
|
R

H
|
H

A

G

A

E

H

N

G

E

H

H

L

-

D

-

R

-

T
x
N

L

L

C

Q

G

G

R

R

M
x
I

P
x
G

G

G

I

D

R

S

-

G

L

L

G

S

E

S

E

R

G

G

R

K

A

K

Q

F

A

A

R

S

A

A

L

L

A

S

A

K

R

F

L

V

K

E

S

E

E

Q

R

N

L

L

D

K

A

D

-

L

-

R

V

V

Y

W

A

T

S

S

P

Q

L

L

E

K

R
x
S

A

T

Q

I

E

Q

T

T

G

A

E

E

P

-

I

-

A

-

A

A

A

L

G

R

V

L

P

P

M

Y

R

E

V

Q

L

W

P

K

A

A

L

L

N

N

E
|
E

I

I

D

D

F

A

G

G

A

V

W

C

S

E

G

E

C

L

S

T

F
x
Y

E

E

L

I

H

R

A

D

D

T

-

Y

-

P

P

E

R

E

W

Y

T

A

L

L

W
x
R

N

E

T

Q

A

D

R
x
K

H

Y

V

Y

T

Y

R

R

P

Y

P

P

G

T

G

G

E

E

T

S

I
x
Y

L

Q

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

E

A

R

S

Q

H

E

G

N

E

-

A

-

R

-

I

V

A

L

L

V

V

I

S

C

H
|
H

A
x
Q

D

A

L

V

I

L

K
x
R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

S

P

A

D

E

A

E

L

M

G

P

R

Y

F

L

E

K

V

C

S

P

P

L

A

H

S

T

I

V

S

L

A

K

V

L

V

T

L

P

G

V

E

A

W
x
Y

G

G

A

C

K

R

V

V

E

E

S

S

I

I

active site: R237 (= R9), H238 (= H10), N244 (≠ T19), S287 (≠ R59), E307 (= E83), H372 (= H149)
binding 6-O-phosphono-beta-D-fructofuranose: I249 (≠ M24), G250 (≠ P25), E307 (= E83), Y318 (≠ F94), R332 (≠ W106), K336 (≠ R110), Y347 (≠ I121), Q373 (≠ A150), R377 (≠ K154)
binding pyrophosphate 2-: Y409 (≠ W186)

Sites not aligning to the query:

binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-(oxan-4-yl)pyridine-4-carboxamide: 30, 34, 35, 137, 139, 144, 148, 151, 152, 199, 202, 226, 228
binding pyrophosphate 2-: 29, 30, 31, 32, 33, 34, 153

6ic0A Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 4 (see paper)
25% identity, 95% coverage: 4:193/199 of query aligns to 230:414/428 of 6ic0A

query
sites
6ic0A

T

T

F

I

F

Y

L

L

V

C

R

R

H

H

A

G

A

E

H

N

G

E

H

H

L

-

D

-

R

-

T

N

L

L

C

Q

G

G

R

R

M

I

P
x
G

G

G

I

D

R

S

-

G

L

L

G

S

E

S

E

R

G

G

R

K

A

K

Q

F

A

A

R

S

A

A

L

L

A

S

A

K

R

F

L

V

K

E

S

E

E

Q

R

N

L

L

D

K

A

D

-

L

-

R

V

V

Y

W

A

T

S

S

P

Q

L

L

E

K

R

S

A

T

Q

I

E

Q

T

T

G

A

E

E

P

-

I

-

A

-

A

A

A

L

G

R

V

L

P

P

M

Y

R

E

V

Q

L

W

P

K

A

A

L

L

N

N

E
|
E

I

I

D

D

F

A

G

G

A

V

W

C

S

E

G

E

C

L

S

T

F
x
Y

E

E

L

I

H

R

A

D

D

T

-

Y

-

P

P

E

R

E

W

Y

T

A

L

L

W
x
R

N

E

T

Q

A

D

R
x
K

H

Y

V

Y

T

Y

R

R

P

Y

P

P

G

T

G

G

E

E

T

S

I
x
Y

L

Q

E

D

A

L

Q

V

A

Q

R

R

A

L

V

E

G

P

A

V

L

I

E

M

E

E

L

L

R

E

A

R

S

Q

H

E

G

N

E

-

A

-

R

-

I

V

A

L

L

V

V

I

S

C

H

H

A
x
Q

D

A

L

V

I

L

K
x
R

L

C

V

L

L

L

A

A

F

Y

A

F

L

L

G

D

L

K

A

S

P

A

D

E

A

E

L

M

G

P

R

Y

F

L

E

K

V

C

S

P

P

L

A

H

S

T

I

V

S

L

A

K

V

L

V

T

L
x
P

G

V

E

A

W
x
Y

G

G

A

C

K

R

V

V

E

E

S

S

I

I

binding 6-O-phosphono-beta-D-fructofuranose: G248 (≠ P25), E305 (= E83), Y316 (≠ F94), R330 (≠ W106), K334 (≠ R110), Y345 (≠ I121), Q371 (≠ A150), R375 (≠ K154)
binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-pyrimidin-5-yl-pyridine-4-carboxamide: P404 (≠ L183)
binding pyrophosphate 2-: Y407 (≠ W186)

Sites not aligning to the query:

binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-pyrimidin-5-yl-pyridine-4-carboxamide: 28, 29, 32, 33, 135, 137, 146, 149, 197, 200, 224, 226
binding pyrophosphate 2-: 26, 27, 28, 29, 30, 31, 32, 151

Query Sequence

>WP_015927792.1 NCBI__GCF_000022085.1:WP_015927792.1
MTTTFFLVRHAAHGHLDRTLCGRMPGIRLGEEGRAQARALAARLKSERLDAVYASPLERA
QETGEPIAAAAGVPMRVLPALNEIDFGAWSGCSFEELHADPRWTLWNTARHVTRPPGGET
ILEAQARAVGALEELRASHGEARIALVSHADLIKLVLAFALGLAPDALGRFEVSPASISA
VVLGEWGAKVESINERVSA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory