SitesBLAST

E190 (= E186) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A188) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G192) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P205) mutation to A: No effect.

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
28% identity, 66% coverage: 47:239/292 of query aligns to 239:454/514 of P02916

query
sites
P02916

S

S

I

I

K

T

P

A

D

D

D

G

Q

N

L

W

I

G

D

D

M

-

E

E

T

K

Y

L

N

S

P

P

F

G

F

Y

V

T

V

V

S

T

P

T

T

G

L

W

K

K

H

N

I

F

T

T

K

R

L

V

L

F

F

T

E

D

T

E

-

G

-

I

Q

Q

Y

K

P

P

-

F

-

L

-

A

L

I

W

F

L

V

W

W

N

-

T

T

M

V

L

V

V

F

S

S

V

L

A

I

A

T

T

V

V

F

L

L

S

T

L

V

F

A

A

V

S

G

V

M

L

V

A

L

A

A

Y

C

A

L

I

V

V

Q

R

W

I

E

R

A

Y

L

R

R

G

G

A

K

A

A

A

V

V

Y

G

R

G

V

A

L

I

L

F

I

L

L

A

P

Y

Y

L

A

V

V

P

P

P

S

S

F

I

I

-

S

L

I

F
x
L

I

I

P

F

L

K

A

G

T

L

I

F

I

N

Q

Q

A

S

Y

F

G

G

L

E

F

I

D

N

S

M

P

M

L

L

-

S

A

A

L

L

I

F

L

G

V

V

Y

K

P

P

-

A

-

W

-

F

-

S

-

D

-

P

-

T

-

A

-

R

T

T

I

M

L
|
L

I

I

P

I

F

V

S
x
N

T

T

W

W

L

L

-
x
G

-

Y

-

P

-

Y

-

M

-

I

-

L

L

C

M

M

G

G

Y

L

F

L

K

K

T

A

I

I

P

P

Y

D

E

D

L

L

E

Y

E
|
E

C

A

A
x
S

L

A

I

M

D

D

G
|
G

A

A

G

G

R

P

W

F

Q

Q

I

N

L

F

T

F

R

K

I

I

I

T

L

L

P
|
P

L

L

A

L

V

I

P

K

G

P

L

L

I

T

S

P

A

L

G

M

I

I

F

A

S

S

L

F

T

A

L

F

C

N

W

F

N

N

E

N

F

F

I

V

Y

L

A

I

L

Q

T

L

F

L

L

T

S

N

S

G

T

G

P

P

N

D

K

R

L334 (≠ F138) mutation to W: Ability to transport lactose in a saturable manner.
L372 (= L165) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ S169) mutation N->K,H: No growth on maltose.
G380 (vs. gap) mutation G->C,S: No growth on maltose.
E401 (= E186) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A188) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G192) mutation G->A,P: No effect.
P420 (= P205) mutation to A: No effect.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
26% identity, 65% coverage: 49:239/292 of query aligns to 218:439/490 of 4ki0F

query
sites
4ki0F

K

R

P

P

D

N

Q

Q

L

I

I

G

D

F

M

Y

E

Q

T

S

Y

I

N

N

-

W

-

G

-

D

-

E

-

K

-

L

-

S

P

P

F

G

F

Y

V

T

V

V

S

T

P

T

T

G

L

W

K

K

H

N

I

F

T

T

K

R

L

V

L

F

F

T

E

D

T

E

-

G

-

I

Q

Q

Y

K

P

P

-

F

-

L

-

A

L

I

W

F

L

V

W

W

N

-

T

T

M

V

L

V

V

F

S

S

V

L

A

I

A

T

T

V

V

F

L

L

S

T

L

V

F

A

A

V

S

G

V

M

L

V

A

L

A

A

Y

C

A

L

I

V

V

Q

R

W

I

E

R

A

Y

L

R

R

G

G

A

K

A

A

A

V

V

Y

G

R

G

V

A

L

I

L

F

I

L

L

A

P

Y
|
Y

L

A

V

V

P

P

P

S

S

F

I

I

-

S

L

I

F

L

I

I

P

F

L

K

A

G

T

L

I

F

I

N

Q

Q

A

S

Y

F

G

G

L

E

F

I

D

N

S

M

P

M

L

L

-

S

A

A

L

L

I

F

L

G

V

V

Y

K

P

P

-

A

-

W

-

F

-

S

-

D

-

P

-

T

-

A

-

R

T

T

I

M

L

L

I

I

P

I

F

V

S
x
N

T

T

W

W

L

L

-
x
G

-

Y

-

P

-
x
Y

-

M

-

I

-

L

L

C

M

M

G

G

Y

L

F

L

K

K

T

A

I

I

P

P

Y

D

E

D

L

L

E

Y

E

E

C

A

A

S

L

A

I

M

D

D

G

G

A

A

G

G

R

P

W

F

Q

Q

I

N

L

F

T

F

R

K

I

I

I

T

L

L

P

P

L

L

A

L

V

I

P

K

G

P

L

L

I

T

S

P

A

L

G

M

I

I

F

A

S
|
S

L

F

T

A

L

F

C
x
N

W

F

N

N

E

N

F

F

I

V

Y

L

A

I

L

Q

T

L

F

L

L

T

S

N

S

G

T

G

P

P

N

D

K

R

binding alpha-D-glucopyranose: Y310 (= Y130), N361 (≠ S169), G365 (vs. gap), Y368 (vs. gap), S418 (= S218), N422 (≠ C222)

Query Sequence

>WP_015928110.1 NCBI__GCF_000022085.1:WP_015928110.1
MAQATADHSEGMAYLETLPRRLVTVYLPLTIILVVLLFPFYWMALTSIKPDDQLIDMETY
NPFFVVSPTLKHITKLLFETQYPLWLWNTMLVSVAATVLSLFASVLAAYAIVRIRYRGAA
AVGGAIFLAYLVPPSILFIPLATIIQAYGLFDSPLALILVYPTILIPFSTWLLMGYFKTI
PYELEECALIDGAGRWQILTRIILPLAVPGLISAGIFSLTLCWNEFIYALTFLSSTPNKT
VPVAVVSEFVDGDIYRWGSLMAGALVGSLPLVILYSFFVEHYVSAMTGAVKE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory