SitesBLAST
Comparing WP_015929413.1 NCBI__GCF_000022085.1:WP_015929413.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
55% identity, 100% coverage: 2:246/246 of query aligns to 1:244/244 of P0AEK2
- GASR 12:15 (≠ GATG 13:16) binding NADP(+)
- T37 (= T38) binding NADP(+)
- NV 59:60 (≠ NL 61:62) binding NADP(+)
- N86 (= N88) binding NADP(+)
- Y151 (= Y153) mutation to F: Defect in the affinity for NADPH.
- YAAAK 151:155 (= YAAAK 153:157) binding NADP(+)
- A154 (= A156) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
- K155 (= K157) mutation to A: Defect in the affinity for NADPH.
- I184 (= I186) binding NADP(+)
- E233 (≠ H235) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.
1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
55% identity, 99% coverage: 3:246/246 of query aligns to 1:243/243 of 1q7bA
- active site: G15 (= G17), E101 (= E104), S137 (= S140), Q147 (= Q150), Y150 (= Y153), K154 (= K157)
- binding calcium ion: E232 (≠ H235), T233 (= T236)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G13), S13 (≠ T15), R14 (≠ G16), T36 (= T38), N58 (= N61), V59 (≠ L62), N85 (= N88), A86 (= A89), G87 (= G90), I88 (= I91), S137 (= S140), Y150 (= Y153), K154 (= K157), P180 (= P183), G181 (= G184), I183 (= I186)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
53% identity, 100% coverage: 1:246/246 of query aligns to 3:247/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G13), S17 (≠ T15), R18 (≠ G16), I20 (≠ L18), T40 (= T38), N62 (= N61), V63 (≠ L62), N89 (= N88), A90 (= A89), I92 (= I91), V139 (≠ I138), S141 (= S140), Y154 (= Y153), K158 (= K157), P184 (= P183), G185 (= G184), I187 (= I186), T189 (≠ S188), M191 (= M190)
1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
55% identity, 99% coverage: 3:246/246 of query aligns to 1:243/243 of 1q7cA
- active site: G15 (= G17), S137 (= S140), Q147 (= Q150), F150 (≠ Y153), K154 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G13), S13 (≠ T15), R14 (≠ G16), A35 (≠ G37), T36 (= T38), L57 (≠ A60), N58 (= N61), V59 (≠ L62), G87 (= G90), I88 (= I91)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
54% identity, 100% coverage: 2:246/246 of query aligns to 1:244/244 of 6t77A
- active site: G16 (= G17), S138 (= S140), Y151 (= Y153)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ T15), R15 (≠ G16), T37 (= T38), L58 (≠ A60), N59 (= N61), V60 (≠ L62), A87 (= A89), G88 (= G90), I89 (= I91)
P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
54% identity, 100% coverage: 2:246/246 of query aligns to 1:244/244 of P0A2C9
- M125 (= M127) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
- A223 (= A225) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
- S224 (= S226) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
53% identity, 99% coverage: 4:246/246 of query aligns to 2:243/243 of 7emgB
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
52% identity, 100% coverage: 1:246/246 of query aligns to 3:243/243 of 4i08A
- active site: G19 (= G17), N113 (= N112), S141 (= S140), Q151 (= Q150), Y154 (= Y153), K158 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G13), S17 (≠ T15), R18 (≠ G16), I20 (≠ L18), T40 (= T38), N62 (= N61), V63 (≠ L62), N89 (= N88), A90 (= A89), G140 (= G139), S141 (= S140), Y154 (= Y153), K158 (= K157), P184 (= P183), G185 (= G184), T189 (≠ S188)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
48% identity, 97% coverage: 7:245/246 of query aligns to 5:246/246 of 3osuA
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
47% identity, 97% coverage: 7:245/246 of query aligns to 2:239/239 of 3sj7A
- active site: G12 (= G17), S138 (= S140), Q148 (= Q150), Y151 (= Y153), K155 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), S10 (≠ T15), R11 (≠ G16), I13 (≠ L18), N31 (≠ S36), Y32 (vs. gap), A33 (vs. gap), G34 (= G37), S35 (≠ T38), A58 (= A60), N59 (= N61), V60 (≠ L62), N86 (= N88), A87 (= A89), T109 (≠ V111), S138 (= S140), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184)
4ag3A Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (fabg) from pseudomonas aeruginosa in complex with NADPH at 1.8a resolution (see paper)
50% identity, 99% coverage: 2:245/246 of query aligns to 8:253/254 of 4ag3A
- active site: G23 (= G17), S148 (= S140), Y161 (= Y153), K165 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G19 (= G13), S21 (≠ T15), R22 (≠ G16), G23 (= G17), I24 (≠ L18), T44 (= T38), L68 (vs. gap), D69 (≠ N61), V70 (≠ L62), N96 (= N88), A97 (= A89), I146 (= I138), S148 (= S140), Y161 (= Y153), K165 (= K157), P191 (= P183), G192 (= G184), F193 (= F185), I194 (= I186), T196 (≠ S188), M198 (= M190), T199 (= T191)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
48% identity, 98% coverage: 4:245/246 of query aligns to 3:247/247 of 4jroC
- active site: G16 (= G17), S142 (= S140), Q152 (= Q150), Y155 (= Y153), K159 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ T15), R15 (≠ G16), G16 (= G17), I17 (≠ L18), N35 (≠ S36), Y36 (vs. gap), N37 (vs. gap), G38 (= G37), S39 (≠ T38), N63 (= N61), V64 (≠ L62), N90 (= N88), A91 (= A89), I93 (= I91), I113 (≠ V111), S142 (= S140), Y155 (= Y153), K159 (= K157), P185 (= P183), I188 (= I186), T190 (≠ S188)
6wprA Crystal structure of a putative 3-oxoacyl-acp reductase (fabg) with NADP(h) from acinetobacter baumannii (see paper)
46% identity, 96% coverage: 9:245/246 of query aligns to 8:243/244 of 6wprA
- active site: G16 (= G17), S138 (= S140), Y151 (= Y153)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ T15), R15 (≠ G16), T37 (= T38), L58 (≠ A60), D59 (≠ N61), V60 (≠ L62), N86 (= N88), A87 (= A89), G88 (= G90), I89 (= I91), I136 (= I138), Y151 (= Y153), K155 (= K157), P181 (= P183)
6t62A Crystal structure of acinetobacter baumannii fabg in complex with NADPH at 1.8 a resolution (see paper)
46% identity, 96% coverage: 9:245/246 of query aligns to 8:243/244 of 6t62A
- active site: G16 (= G17), S138 (= S140), Y151 (= Y153)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ T15), R15 (≠ G16), A36 (≠ G37), T37 (= T38), L58 (≠ A60), D59 (≠ N61), V60 (≠ L62), N86 (= N88), A87 (= A89), G88 (= G90), I89 (= I91), I136 (= I138), S137 (≠ G139), S138 (= S140), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184), I184 (= I186), M188 (= M190)
4bo4C Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (fabg) from pseudomonas aeruginosa in complex with n-(2-methoxyphenyl)-3,4- dihydro-2h-quinoline-1-carboxamide at 2.7a resolution (see paper)
50% identity, 99% coverage: 2:245/246 of query aligns to 14:254/255 of 4bo4C
3tzcA Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (fabg)(y155f) from vibrio cholerae (see paper)
47% identity, 99% coverage: 1:243/246 of query aligns to 3:223/224 of 3tzcA
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
48% identity, 100% coverage: 1:246/246 of query aligns to 1:247/247 of P73574
- A14 (= A14) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (≠ A148) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K157) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (= F185) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (= N195) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
4dmmB 3-oxoacyl-[acyl-carrier-protein] reductase from synechococcus elongatus pcc 7942 in complex with NADP
49% identity, 99% coverage: 2:245/246 of query aligns to 1:239/240 of 4dmmB
- active site: G16 (= G17), S142 (= S140), Q152 (= Q150), Y155 (= Y153), K159 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ T15), R15 (≠ G16), G16 (= G17), I17 (≠ L18), A37 (vs. gap), S38 (= S36), S39 (≠ G37), A62 (= A60), D63 (≠ N61), V64 (≠ L62), N90 (= N88), A91 (= A89), L113 (≠ V111), I140 (= I138), S142 (= S140), Y155 (= Y153), K159 (= K157), P185 (= P183), G186 (= G184), I188 (= I186), T190 (≠ S188), M192 (= M190)
4bnzA Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (fabg) from pseudomonas aeruginosa in complex with 1-methyl-n-phenylindole- 3-carboxamide at 2.5a resolution (see paper)
48% identity, 99% coverage: 2:245/246 of query aligns to 3:240/241 of 4bnzA
5vt6A Crystal structure of acetoacetyl-coa reductase from burkholderia pseudomallei 1710b complexed with NADP
45% identity, 97% coverage: 7:245/246 of query aligns to 2:244/245 of 5vt6A
- active site: G12 (= G17), D102 (≠ E104), S138 (= S140), Y151 (= Y153), K155 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), G11 (= G16), G12 (= G17), L13 (= L18), H32 (≠ G37), S33 (≠ T38), N36 (≠ D41), V58 (≠ A60), D59 (≠ N61), V60 (≠ L62), N86 (= N88), A87 (= A89), G88 (= G90), I89 (= I91), I136 (= I138), Y151 (= Y153), K155 (= K157), P181 (= P183), Y183 (≠ F185), L184 (≠ I186), T186 (≠ S188)
Query Sequence
>WP_015929413.1 NCBI__GCF_000022085.1:WP_015929413.1
MFDLTGRKALVTGATGGLGGAIARAFHAQGAEVAISGTRRDALDALAAEFGGERVHVVEA
NLADTAAVEGLVPAAEAALGGLDILVNNAGITRDNLFLRMKDEEWDSVIAVNLTAAFRLS
RAAVKGMMRRRYGRIVNIGSVVGSTGNAGQGNYAAAKAGLVGLTKALAAEVASRKITVNC
IAPGFIASPMTDALNEKQREGILATVPMGRLGEGAEVAAAAVYLASDEAAYVTGHTLHVN
GGMAML
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory