SitesBLAST
Comparing WP_015930053.1 NCBI__GCF_000022085.1:WP_015930053.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
48% identity, 97% coverage: 3:245/250 of query aligns to 4:245/248 of Q9KJF1
- S15 (≠ R14) binding NAD(+)
- D36 (= D35) binding NAD(+)
- D62 (= D62) binding NAD(+)
- I63 (≠ V63) binding NAD(+)
- N89 (= N89) binding NAD(+)
- Y153 (= Y153) binding NAD(+)
- K157 (= K157) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
48% identity, 97% coverage: 3:245/250 of query aligns to 3:244/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), M16 (≠ I16), D35 (= D35), I36 (≠ L36), I62 (≠ V63), N88 (= N89), G90 (= G91), I138 (= I139), S140 (= S141), Y152 (= Y153), K156 (= K157), I185 (≠ V186)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
44% identity, 96% coverage: 7:245/250 of query aligns to 11:247/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G11), R18 (= R14), G19 (= G15), I20 (= I16), D39 (= D35), R40 (≠ L36), C63 (≠ A61), I65 (≠ V63), N91 (= N89), G93 (= G91), I94 (= I92), V114 (= V112), Y155 (= Y153), K159 (= K157), I188 (≠ V186), T190 (= T188), T193 (≠ F191)
6t62A Crystal structure of acinetobacter baumannii fabg in complex with NADPH at 1.8 a resolution (see paper)
41% identity, 96% coverage: 3:242/250 of query aligns to 4:240/244 of 6t62A
- active site: G16 (= G15), S138 (= S141), Y151 (= Y153)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), S14 (≠ A13), R15 (= R14), A36 (= A39), T37 (≠ A40), L58 (≠ A61), D59 (= D62), V60 (= V63), N86 (= N89), A87 (= A90), G88 (= G91), I89 (= I92), I136 (= I139), S137 (= S140), S138 (= S141), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184), I184 (≠ V186), M188 (≠ L190)
6wprA Crystal structure of a putative 3-oxoacyl-acp reductase (fabg) with NADP(h) from acinetobacter baumannii (see paper)
41% identity, 96% coverage: 3:242/250 of query aligns to 4:240/244 of 6wprA
- active site: G16 (= G15), S138 (= S141), Y151 (= Y153)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), S14 (≠ A13), R15 (= R14), T37 (≠ A40), L58 (≠ A61), D59 (= D62), V60 (= V63), N86 (= N89), A87 (= A90), G88 (= G91), I89 (= I92), I136 (= I139), Y151 (= Y153), K155 (= K157), P181 (= P183)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
42% identity, 96% coverage: 3:242/250 of query aligns to 7:243/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G11), S17 (≠ A13), R18 (= R14), I20 (= I16), T40 (≠ A40), N62 (≠ D62), V63 (= V63), N89 (= N89), A90 (= A90), I92 (= I92), V139 (≠ I139), S141 (= S141), Y154 (= Y153), K158 (= K157), P184 (= P183), G185 (= G184), I187 (≠ V186), T189 (= T188), M191 (≠ L190)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
41% identity, 96% coverage: 3:242/250 of query aligns to 4:240/244 of 6t77A
- active site: G16 (= G15), S138 (= S141), Y151 (= Y153)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), S14 (≠ A13), R15 (= R14), T37 (≠ A40), L58 (≠ A61), N59 (≠ D62), V60 (= V63), A87 (= A90), G88 (= G91), I89 (= I92)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
42% identity, 96% coverage: 3:242/250 of query aligns to 6:241/244 of 4nbuB
- active site: G18 (= G15), N111 (= N113), S139 (= S141), Q149 (= Q150), Y152 (= Y153), K156 (= K157)
- binding acetoacetyl-coenzyme a: D93 (= D95), K98 (= K100), S139 (= S141), N146 (≠ G147), V147 (≠ F148), Q149 (= Q150), Y152 (= Y153), F184 (≠ I185), M189 (≠ L190), K200 (≠ R201)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G11), N17 (≠ R14), G18 (= G15), I19 (= I16), D38 (= D35), F39 (≠ L36), V59 (≠ A61), D60 (= D62), V61 (= V63), N87 (= N89), A88 (= A90), G89 (= G91), I90 (= I92), T137 (≠ I139), S139 (= S141), Y152 (= Y153), K156 (= K157), P182 (= P183), F184 (≠ I185), T185 (≠ V186), T187 (= T188), M189 (≠ L190)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
42% identity, 96% coverage: 3:242/250 of query aligns to 7:239/243 of 4i08A
- active site: G19 (= G15), N113 (= N113), S141 (= S141), Q151 (= Q150), Y154 (= Y153), K158 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G11), S17 (≠ A13), R18 (= R14), I20 (= I16), T40 (≠ A40), N62 (≠ D62), V63 (= V63), N89 (= N89), A90 (= A90), G140 (≠ S140), S141 (= S141), Y154 (= Y153), K158 (= K157), P184 (= P183), G185 (= G184), T189 (= T188)
4nbwA Crystal structure of fabg from plesiocystis pacifica (see paper)
48% identity, 98% coverage: 4:247/250 of query aligns to 1:253/253 of 4nbwA
- active site: G12 (= G15), S146 (= S141), Y159 (= Y153), K163 (= K157)
- binding nicotinamide-adenine-dinucleotide: G8 (= G11), N11 (≠ R14), G12 (= G15), I13 (= I16), D32 (= D35), L33 (= L36), V57 (≠ T60), D58 (= D62), V59 (= V63), N85 (= N89), A86 (= A90), G87 (= G91), S146 (= S141), Y159 (= Y153), K163 (= K157), I192 (≠ V186), T194 (= T188)
P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
40% identity, 96% coverage: 3:242/250 of query aligns to 4:240/244 of P0AEK2
- GASR 12:15 (≠ GAAR 11:14) binding NADP(+)
- T37 (≠ A40) binding NADP(+)
- NV 59:60 (≠ DV 62:63) binding NADP(+)
- N86 (= N89) binding NADP(+)
- Y151 (= Y153) mutation to F: Defect in the affinity for NADPH.
- YAAAK 151:155 (≠ YSAAK 153:157) binding NADP(+)
- A154 (= A156) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
- K155 (= K157) mutation to A: Defect in the affinity for NADPH.
- I184 (≠ V186) binding NADP(+)
- E233 (≠ Q235) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.
1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
40% identity, 96% coverage: 3:242/250 of query aligns to 3:239/243 of 1q7bA
- active site: G15 (= G15), E101 (≠ D105), S137 (= S141), Q147 (= Q150), Y150 (= Y153), K154 (= K157)
- binding calcium ion: E232 (≠ Q235), T233 (≠ L236)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G11), S13 (≠ A13), R14 (= R14), T36 (≠ A40), N58 (≠ D62), V59 (= V63), N85 (= N89), A86 (= A90), G87 (= G91), I88 (= I92), S137 (= S141), Y150 (= Y153), K154 (= K157), P180 (= P183), G181 (= G184), I183 (≠ V186)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
43% identity, 94% coverage: 7:242/250 of query aligns to 7:243/246 of 3osuA
P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
40% identity, 96% coverage: 3:242/250 of query aligns to 4:240/244 of P0A2C9
- M125 (= M128) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
- A223 (= A225) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
- S224 (≠ G226) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
43% identity, 95% coverage: 6:242/250 of query aligns to 7:244/247 of 4jroC
- active site: G16 (= G15), S142 (= S141), Q152 (= Q150), Y155 (= Y153), K159 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), S14 (≠ A13), R15 (= R14), G16 (= G15), I17 (= I16), N35 (vs. gap), Y36 (≠ L34), N37 (≠ D35), G38 (≠ L36), S39 (≠ D37), N63 (≠ D62), V64 (= V63), N90 (= N89), A91 (= A90), I93 (= I92), I113 (≠ V112), S142 (= S141), Y155 (= Y153), K159 (= K157), P185 (= P183), I188 (≠ V186), T190 (= T188)
1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
39% identity, 96% coverage: 3:242/250 of query aligns to 3:239/243 of 1q7cA
- active site: G15 (= G15), S137 (= S141), Q147 (= Q150), F150 (≠ Y153), K154 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G11), S13 (≠ A13), R14 (= R14), A35 (= A39), T36 (≠ A40), L57 (≠ A61), N58 (≠ D62), V59 (= V63), G87 (= G91), I88 (= I92)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
43% identity, 94% coverage: 7:242/250 of query aligns to 4:236/239 of 3sj7A
- active site: G12 (= G15), S138 (= S141), Q148 (= Q150), Y151 (= Y153), K155 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), S10 (≠ A13), R11 (= R14), I13 (= I16), N31 (vs. gap), Y32 (≠ L34), A33 (≠ D35), G34 (≠ L36), S35 (≠ D37), A58 (= A61), N59 (≠ D62), V60 (= V63), N86 (= N89), A87 (= A90), T109 (≠ V112), S138 (= S141), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184)
7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
38% identity, 96% coverage: 3:242/250 of query aligns to 3:239/243 of 7emgB
4cqlI Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD (see paper)
40% identity, 95% coverage: 6:242/250 of query aligns to 10:248/251 of 4cqlI
- active site: G19 (= G15), S146 (= S141), Y159 (= Y153), K163 (= K157)
- binding nicotinamide-adenine-dinucleotide: S18 (≠ R14), G19 (= G15), I20 (= I16), D39 (= D35), L40 (= L36), A64 (= A61), D65 (= D62), V66 (= V63), C93 (≠ N89), A94 (= A90), G95 (= G91), I96 (= I92), V116 (= V112), I144 (= I139), S146 (= S141), Y159 (= Y153), K163 (= K157), P189 (= P183), G190 (= G184), I192 (≠ V186), T194 (= T188), M196 (≠ L190)
Q92506 (3R)-3-hydroxyacyl-CoA dehydrogenase; 17-beta-hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; HSD17B8; 3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit; KAR alpha subunit; 3-oxoacyl-[acyl-carrier-protein] reductase; Estradiol 17-beta-dehydrogenase 8; Protein Ke6; Ke6; Short chain dehydrogenase/reductase family 30C member 1; Testosterone 17-beta-dehydrogenase 8; EC 1.1.1.n12; EC 1.1.1.62; EC 1.1.1.239 from Homo sapiens (Human) (see 2 papers)
40% identity, 95% coverage: 6:242/250 of query aligns to 13:258/261 of Q92506
- 15:23 (vs. 8:16, 67% identical) binding NAD(+)
- D42 (= D35) mutation to A: Reduced NADH-dependent reductase activity with acetoacetyl-CoA. Reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Increases NADPH-dependent reductase activities. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- DL 42:43 (= DL 35:36) binding NAD(+)
- ADV 74:76 (= ADV 61:63) binding NAD(+)
- R148 (≠ Y133) mutation to E: No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- V158 (≠ R142) to L: in a breast cancer sample; somatic mutation
- Y169 (= Y153) mutation to A: Strongly reduced NADH-dependent reductase activity with acetoacetyl-CoA. Strongly reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Decreases NADPH-dependent reductase activity with acetoacetyl-CoA, but increases NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- YAASK 169:173 (≠ YSAAK 153:157) binding NAD(+)
- K173 (= K157) mutation to A: Abolishes NADH-dependent reductase activity with acetoacetyl-CoA. Strongly reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Slightly decreases NADPH-dependent reductase activity with acetoacetyl-CoA, but increases NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- R189 (= R173) mutation to E: No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- IAT 202:204 (≠ VET 186:188) binding NAD(+)
Query Sequence
>WP_015930053.1 NCBI__GCF_000022085.1:WP_015930053.1
MAEKDVALITGAARGIGFGIAESLARAGYDIALLDLDRAAVEGAARQIGDLGGGRVVATT
ADVTNRTEVEAAVAAVHAAFGRIDVLVNNAGIVRDRRLVKMDESDWDAVIAVNLKSQFLM
CKAVVPGMIERAYGRIVNISSRAWLGGFGQANYSAAKGGVVSLTRSLAIELARNGITVNA
IAPGIVETPLFQNFEPEVQERLKKSVPMQRIGTPKDIAQAAVFFAGRASSYITGQLIYVC
GGRSLSSPSV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory