SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_015931290.1 NCBI__GCF_000022085.1:WP_015931290.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7kb1C Complex of o-acety-l-homoserine aminocarboxypropyltransferase (mety) from thermotoga maritima and a key reaction intermediate (see paper)
55% identity, 98% coverage: 7:424/426 of query aligns to 6:423/428 of 7kb1C

query
sites
7kb1C

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binding pyridoxal-5'-phosphate: Y57 (= Y57), R59 (= R59)
binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: G87 (= G87), Q88 (≠ H88), Y112 (= Y112), N160 (= N159), D185 (= D184), S206 (= S206), T208 (= T208), K209 (= K209), N369 (= N370), I370 (= I371), R404 (= R405)

7kb1A Complex of o-acety-l-homoserine aminocarboxypropyltransferase (mety) from thermotoga maritima and a key reaction intermediate (see paper)
55% identity, 98% coverage: 7:424/426 of query aligns to 6:423/428 of 7kb1A

query
sites
7kb1A

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binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: Y57 (= Y57), R59 (= R59), G87 (= G87), Q88 (≠ H88), Y112 (= Y112), N160 (= N159), D185 (= D184), S206 (= S206), T208 (= T208), K209 (= K209), N369 (= N370), I370 (= I371), R404 (= R405)

8wkoA Crystal structure of o-acetylhomoserine sulfhydrylase from lactobacillus plantarum in the closed form (see paper)
52% identity, 98% coverage: 8:425/426 of query aligns to 9:423/425 of 8wkoA

query
sites
8wkoA

F

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I

S

S

I

V

G

G

I

V

E

E

D

N

P

A

A

D

D

D

L

L

I

I

A

A

D

D

L

L

D

D

Q

Q

A

A

L

L

A

A

binding (2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid: G87 (= G87), S88 (≠ H88), Y112 (= Y112), E155 (= E155), D184 (= D184), T186 (= T186), S206 (= S206), A207 (= A207), T208 (= T208), F209 (= F210), G212 (= G213), M217 (≠ I218), V369 (≠ I371), A370 (≠ G372)
binding proline: H213 (= H214), Q284 (≠ N286), S288 (≠ T290)

2ctzA Crystal structure of o-acetyl homoserine sulfhydrylase from thermus thermophilus hb8
52% identity, 98% coverage: 8:425/426 of query aligns to 3:421/421 of 2ctzA

query
sites
2ctzA

F

F

D

E

T

T

L

L

A

Q

I

L

H

H

A

A

G

G

A

Y

A

E

P

P

D

E

A

P

A

T

T

T

G

L

A

S

R

R

A

Q

T

V

P

P

I

I

Y

Y

Q

P

T

T

T

T

S

S

F

Y

V

V

F

F

D

K

D

S

V

P

D

E

H

H

A

A

A

A

S

N

L

L

F

F

G

A

L

L

Q

K

A

E

F

F

G

G

N

N

I

I

Y

Y

S

S

R
|
R

I

I

G

M

N

N

P

P

T

T

N

V

A

D

V

V

L

L

E

E

E

K

R

R

V

L

A

A

A

A

L

L

E

E

G

G

G

G

T

K

A

A

A

A

L

L

A

A

V

T

A

A

S
|
S

G
|
G

H
|
H

A

A

A

A

Q
|
Q

F

F

L

L

V

A

M

L

H

T

A

T

L

L

L

A

Q

Q

P

A

G

G

D

D

E

N

F

I

I

V

A

S

A

T

N

P

K

N

L

L

Y
|
Y

G

G

G

G

S

T

I

F

N

N

Q

Q

F

F

N

K

H

V

S

T

Y

L

K

K

N

R

F

L

G

G

W

I

S

E

V

V

A

R

W

F

A

T

D

S

T

R

D

E

D

E

-

R

P

P

A

E

S

E

F

F

E

L

A

A

A

L

I

T

T

D

P

E

R

K

T

T

K

R

A

A

I

W

F

W

V

V

E

E

S

S

I

I

A

G

N

N

P

P

G

A

G

L

V

N

I

I

T

P

D

D

L

L

A

E

A

A

I

L

A

A

A

Q

V

A

A

A

K

R

R

E

H

K

N

G

I

V

P

A

F

L

I

I

V

V

D
|
D

N

N

T
|
T

M

F

A

G

T

M

P

G

-

G

Y

Y

L

L

I

L

R

R

P

P

F

L

E

A

H

W

G

G

A

A

D

A

I

L

V

V

V

T

H

H

S
|
S

A

L

T
|
T

K
|
K

F

W

L

V

G

G

G

G

H

H

G

G

N

A

S

V

I

I

G

A

G

G

L

A

I

I

V

V

D

D

G

G

G

G

T

N

F

F

A

P

W

W

A

E

G

G

D

G

D

-

R

R

Y

Y

P

P

M

L

L

L

S

T

K

E

P

P

R

Q

P

P

E

G

Y

Y

G

H

G

G

M

L

V

R

L

L

S

T

E

E

T

A

F

F

G

G

N

E

F

L

G

A

F

F

A

I

I

V

A

K

V

A

R

R

V

V

L

D

G

G

L

L

R

R

D

D

L

Q

G

G

P

Q

A

A

L

L

S

G

P

P

F

F

N

E

A

A

F

W

L

V

I

V

L

L

N

L

G

G

I

M

E

E

T

T

L

L

P

S

L

L

R

R

M

A

Q

E

R

R

H

H

S

V

D

E

N

N

A

T

R

L

V

H

V

L

A

A

Q

H

H

W

L

L

E

L

R

E

H

Q

P

P

E

Q

V

V

S

A

W

W

V

V

S

N

Y

Y

P

P

G

G

L

L

E

P

T

H

D

H

R

P

Y

H

H

H

A

D

L

R

H

A

K

Q

R

K

Y

Y

C

F

P

K

L

G

G

K

A

P

G

G

A

A

V

V

F

L

T

T

F

F

G

G

L

L

K

K

G

G

G

G

Y

Y

E

E

A

A

G

A

V

K

K

R

L

F

V

I

S

S

N

R

L

L

K

K

L

L

F

I

S

S

H

H

L

L

A

A

N

N

I

V

G

G

D

D

T

T

R

R

S

T

L

L

V

A

I

I

H

H

P

P

A

A

S

S

T

T

T

T

H

H

R

S

Q

Q

L

L

T

S

D

P

E

E

Q

E

K

Q

V

A

R

Q

A

A

G

G

A

V

G

S

P

P

E

E

V

M

V

V

R

R

L

L

S

S

I

V

G

G

I

L

E

E

D

H

P

V

A

E

D

D

L

L

I

K

A

A

D

E

L

L

D

K

Q

E

A

A

L

L

A

A

active site: R54 (= R59), Y107 (= Y112), D180 (= D184), K206 (= K209)
binding pyridoxal-5'-phosphate: S81 (= S86), G82 (= G87), H83 (= H88), Q86 (= Q91), Y107 (= Y112), D180 (= D184), T182 (= T186), S203 (= S206), T205 (= T208), K206 (= K209)

Q5SK88 O-acetyl-L-homoserine sulfhydrylase 1; OAH-sulfhydrylase 1; EC 2.5.1.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
52% identity, 98% coverage: 8:425/426 of query aligns to 3:421/421 of Q5SK88

query
sites
Q5SK88

F

F

D

E

T

T

L

L

A

Q

I

L

H

H

A

A

G

G

A

Y

A

E

P

P

D

E

A

P

A

T

T

T

G

L

A

S

R

R

A

Q

T

V

P

P

I

I

Y

Y

Q

P

T

T

T

T

S

S

F

Y

V

V

F

F

D

K

D

S

V

P

D

E

H

H

A

A

A

A

S

N

L

L

F

F

G

A

L

L

Q

K

A

E

F

F

G

G

N

N

I

I

Y

Y

S

S

R

R

I

I

G

M

N

N

P

P

T

T

N

V

A

D

V

V

L

L

E

E

E

K

R

R

V

L

A

A

A

A

L

L

E

E

G

G

G

G

T

K

A

A

A

A

L

L

A

A

V

T

A

A

S

S

G

G

H

H

A

A

A

A

Q

Q

F

F

L

L

V

A

M

L

H

T

A

T

L

L

L

A

Q

Q

P

A

G

G

D

D

E

N

F

I

I

V

A

S

A

T

N

P

K

N

L

L

Y

Y

G

G

G

G

S

T

I

F

N

N

Q

Q

F

F

N

K

H

V

S

T

Y

L

K

K

N

R

F

L

G

G

W

I

S

E

V

V

A

R

W

F

A

T

D

S

T

R

D

E

D

E

-

R

P

P

A

E

S

E

F

F

E

L

A

A

A

L

I

T

T

D

P

E

R

K

T

T

K

R

A

A

I

W

F

W

V

V

E

E

S

S

I

I

A

G

N

N

P

P

G

A

G

L

V

N

I

I

T

P

D

D

L

L

A

E

A

A

I

L

A

A

A

Q

V

A

A

A

K

R

R

E

H

K

N

G

I

V

P

A

F

L

I

I

V

V

D

D

N

N

T

T

M

F

A

G

T

M

P

G

-

G

Y

Y

L

L

I

L

R

R

P

P

F

L

E

A

H

W

G

G

A

A

D

A

I

L

V

V

V

T

H

H

S

S

A

L

T

T

K
|
K

F

W

L

V

G

G

G

G

H

H

G

G

N

A

S

V

I

I

G

A

G

G

L

A

I

I

V

V

D

D

G

G

G

G

T

N

F

F

A

P

W

W

A

E

G

G

D

G

D

-

R

R

Y

Y

P

P

M

L

L

L

S

T

K

E

P

P

R

Q

P

P

E

G

Y

Y

G

H

G

G

M

L

V

R

L

L

S

T

E

E

T

A

F

F

G

G

N

E

F

L

G

A

F

F

A

I

I

V

A

K

V

A

R

R

V

V

L

D

G

G

L

L

R

R

D

D

L

Q

G

G

P

Q

A

A

L

L

S

G

P

P

F

F

N

E

A

A

F

W

L

V

I

V

L

L

N

L

G

G

I

M

E

E

T

T

L

L

P

S

L

L

R

R

M

A

Q

E

R

R

H

H

S

V

D

E

N

N

A

T

R

L

V

H

V

L

A

A

Q

H

H

W

L

L

E

L

R

E

H

Q

P

P

E

Q

V

V

S

A

W

W

V

V

S

N

Y

Y

P

P

G

G

L

L

E

P

T

H

D

H

R

P

Y

H

H

H

A

D

L

R

H

A

K

Q

R

K

Y

Y

C

F

P

K

L

G

G

K

A

P

G

G

A

A

V

V

F

L

T

T

F

F

G

G

L

L

K

K

G

G

G

G

Y

Y

E

E

A

A

G

A

V

K

K

R

L

F

V

I

S

S

N

R

L

L

K

K

L

L

F

I

S

S

H

H

L

L

A

A

N

N

I

V

G

G

D

D

T

T

R

R

S

T

L

L

V

A

I

I

H

H

P

P

A

A

S

S

T

T

T

T

H

H

R

S

Q

Q

L

L

T

S

D

P

E

E

Q

E

K

Q

V

A

R

Q

A

A

G

G

A

V

G

S

P

P

E

E

V

M

V

V

R

R

L

L

S

S

I

V

G

G

I

L

E

E

D

H

P

V

A

E

D

D

L

L

I

K

A

A

D

E

L

L

D

K

Q

E

A

A

L

L

A

A

K206 (= K209) modified: N6-(pyridoxal phosphate)lysine

O13326 Homocysteine synthase; O-acetylhomoserine sulfhydrylase; OAH SHL; OAH sulfhydrylase; EC 2.5.1.49 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
51% identity, 98% coverage: 8:424/426 of query aligns to 8:426/429 of O13326

query
sites
O13326

F

F

D

E

T

T

L

L

A

Q

I

L

H

H

A

A

G

G

A

Q

A

E

P

P

D

D

A

A

A

A

T

T

G

S

A

S

R

R

A

A

T

V

P

P

I

I

Y

Y

Q

A

T

T

T

T

S

S

F

Y

V

V

F

F

D

R

D

D

V

C

D

D

H

H

A

G

A

G

S

R

L

L

F

F

G

G

L

L

Q

Q

A

E

F

P

G

G

N

Y

I

I

Y

Y

S

S

R

R

I

M

G

M

N

N

P

P

T

T

N

A

A

D

V

V

L

F

E

E

E

K

R

R

V

I

A

A

A

A

L

L

E

E

G

H

G

G

T

A

A

A

A

A

L

I

A

A

V

T

A

S

S

S

G

G

H

T

A

S

A

A

Q

L

F

F

L

M

V

A

M

L

H

T

A

T

L

L

L

A

Q

K

P

A

G

G

D

D

E

N

F

I

I

V

A

S

A

T

N

S

K

Y

L

L

Y

Y

G

G

G

G

S

T

I

Y

N

N

Q

L

F

F

N

K

H

V

S

T

Y

L

K

P

N

R

F

L

G

G

W

I

S

T

V

T

A

K

W

F

A

V

D

N

T

G

D

D

D

D

P

P

A

N

S

D

F

L

E

A

A

A

A

Q

I

I

T

D

P

E

R

N

T

T

K

K

A

A

I

V

F

Y

V

V

E

E

S

S

I

I

A

G

N

N

P

P

G

M

G

Y

V

N

I

V

T

P

D

D

L

F

A

E

A

R

I

I

A

A

A

E

V

V

A

A

K

H

R

A

H

A

N

G

I

V

P

P

F

L

I

M

V

V

D

D

N

N

T

T

M

F

A

G

T

G

P

G

-

G

Y

Y

L

L

I

V

R

R

P

P

F

I

E

D

H

H

G

G

A

A

D

D

I

I

V

V

V

T

H

H

S

S

A

A

T

T

K

K

F

W

L

I

G

G

G

G

H

H

G

G

N

T

S

T

I

I

G

G

G

G

L

V

I

I

V

V

D

D

G

S

G

G

T

K

F

F

A

D

W

W

A

K

G

K

D

N

D

S

-

K

R

R

Y

F

P

P

M

E

L

F

S

N

K

E

P

P

R

H

P

P

E

G

Y

Y

G

H

G

G

M

M

V

V

L

F

S

T

E

E

T

T

F

F

G

G

N

N

F

L

G

A

F

Y

A

A

I

F

A

A

V

C

R

R

V

T

L

Q

G

T

L

L

R

R

D

D

L

V

G

G

P

G

A

N

L

A

S

N

P

P

F

F

N

G

A

V

F

F

L

L

I

L

L

L

N

Q

G

G

I

L

E

E

T

T

L

L

P

S

L

L

R

R

M

M

Q

E

R

R

H

H

S

V

D

Q

N

N

A

A

R

F

V

A

V

L

A

A

Q

K

H

Y

L

L

E

E

R

K

H

H

P

P

E

K

V

V

S

N

W

W

V

V

S

S

Y

Y

P

P

G

G

L

L

E

E

T

S

D

H

R

V

Y

S

H

H

A

K

L

L

H

A

K

K

R

K

Y

Y

C

L

P

K

L

N

G

G

A

Y

G

G

A

A

V

V

F

L

T

S

F

F

G

G

L

A

K

K

G

G

G

G

Y

P

E

D

A

Q

G

S

V

R

K

K

L

V

V

V

S

N

N

A

L

L

K

K

L

L

F

A

S

S

H

Q

L

L

A

A

N

N

I

V

G

G

D

D

T

A

R

K

S

T

L

L

V

V

I

I

H

A

P

P

A

A

S

Y

T

T

T

T

H

H

R

L

Q

Q

L

L

T

T

D

D

E

E

Q

E

K

Q

V

I

R

S

A

A

G

G

A

V

G

T

P

K

E

D

V

L

V

I

R

R

L

V

S

A

I

V

G
|
G

I

I

E

E

D

H

P

I

A

D

D

D

L

I

I

I

A

A

D

D

L

F

D

A

Q

Q

A

A

L

L

G411 (= G409) mutation to D: Impairs homocysteine synthase activity.

8erjB Crystal structure of fub7 in complex with e-2-aminocrotonate (see paper)
48% identity, 98% coverage: 8:424/426 of query aligns to 5:423/428 of 8erjB

query
sites
8erjB

F

F

D

E

T

T

L

L

A

Q

I

L

H

H

A

A

G

G

A

Y

A

T

P

P

D

D

A

P

A

H

T

T

G

R

A

S

R

T

A

A

T

V

P

P

I

I

Y

Y

Q

A

T

T

T

S

S

S

F

Y

V

T

F

F

D

N

D

D

V

S

D

A

H

H

A

G

A

A

S

R

L

L

F

F

G

G

L

L

Q

K

A

E

F

L

G

G

N

N

I

I

Y

Y

S

S

R

R

I

L

G

M

N

N

P

P

T

T

N

V

A

D

V

V

L

F

E

E

E

K

R

R

V

I

A

A

A

A

L

L

E

E

G

G

G

G

T

I

A

A

A

A

L

A

A

A

V

T

A

S

S

S

G
|
G

H
x
Q

A

A

A

A

Q
|
Q

F

F

L

L

V

T

M

I

H

A

A

T

L

L

L

A

Q

K

P

A

G

G

D

D

E

N

F

I

I

V

A

A

A

S

N

S

K

H

L

L

Y
|
Y

G

G

G

G

S

T

I

Y

N

N

Q

Q

F

L

N

N

H

V

S

L

Y

L

K

P

N

R

F

F

G

G

W

I

S

K

V

T

A

K

W

F

A

V

D

R

T

S

D

G

D

K

P

L

A

E

S

D

F

Y

E

A

A

A

A

A

I

I

T

D

P

D

R

Q

T

T

K

R

A

A

I

I

F

Y

V

V

E

E

S

S

I

M

A

S

N

N

P

P

G

D

G

Y

V

V

I

V

T

P

D

D

L

F

A

E

A

G

I

I

A

A

A

K

V

I

A

A

K

H

R

E

H

H

N

G

I

I

P

P

F

L

I

V

V

V

D
|
D

N

N

T

T

M

L

-

G

A

A

T

G

P

G

Y

Y

L

Y

I

I

R

R

P

P

F

I

E

E

H

H

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

A

T

T

K
|
K

F

W

L

I

G

G

G

G

H

H

G

G

N

T

S

T

I

I

G

G

G

G

L

V

I

I

V

V

D

D

G

S

G

G

T

R

F

F

A

N

W

W

A

N

G

K

-

H

D

S

D

D

R

R

Y

F

P

P

M

E

L

M

S

V

K

E

P

P

R

S

P

P

E

S

Y

Y

G

H

G

G

M

L

V

K

L

Y

S

W

E

E

T

A

F

F

G

G

N

P

F

A

G

T

F

F

A

I

I

T

A

R

V

I

R

R

V

V

L

E

G

M

L

L

R

R

D

D

L

I

G

G

P

A

A

C

L

L

S

S

P

P

F

F

N

S

A

A

F

Q

L

Q

I

L

L

L

N

L

G

G

I

I

E

E

T

T

L

L

P

G

L

L

R

R

M

A

Q

E

R

R

H

H

S

A

D

Q

N

N

A

T

R

E

V

K

V

L

A

S

Q

K

H

Y

L

F

E

E

R

S

H

S

P

P

E

N

V

V

S

S

W

W

V

V

S

L

Y

W

P

P

G

G

L

S

E

E

T

S

D

H

R

P

Y

T

H

Y

A

S

L

Q

H

A

K

K

R

K

Y

Y

C

L

P

T

L

R

G

G

A

F

G

G

A

A

V

M

F

L

T

S

F

I

G

G

L

V

K

K

G

G

G

D

Y

A

E

S

A

A

G

G

V

S

K

K

L

V

V

V

S

D

N

G

L

L

K

K

L

L

F

V

S

S

H

N

L

L

A
|
A

N
|
N

I

V

G

G

D

D

T

A

R

K

S

S

L

L

V

A

I

I

H

H

P

P

A

W

S

S

T

T

T
|
T

H

H

R

E

Q

Q

L

L

T

S

D

E

E

D

Q

E

K

R

V

L

R

A

A

S

G

G

A

V

G

T

P

E

E

D

V

M

V

I

R
|
R

L

I

S

S

I

V

G

G

I

I

E

E

D

H

P

V

A

D

D

D

L

I

I

I

A

A

D

D

L

F

D

E

Q

Q

A

S

L

F

binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]but-3-enoic acid: G84 (= G87), Q85 (≠ H88), Q88 (= Q91), Y109 (= Y112), D181 (= D184), S204 (= S206), K207 (= K209), A368 (= A369), N369 (= N370), T384 (= T385), R404 (= R405)

8erjA Crystal structure of fub7 in complex with e-2-aminocrotonate (see paper)
48% identity, 98% coverage: 8:424/426 of query aligns to 5:423/428 of 8erjA

query
sites
8erjA

F

F

D

E

T

T

L

L

A

Q

I

L

H

H

A

A

G

G

A

Y

A

T

P

P

D

D

A

P

A

H

T

T

G

R

A

S

R

T

A

A

T

V

P

P

I

I

Y

Y

Q

A

T

T

T

S

S

S

F

Y

V

T

F

F

D

N

D

D

V

S

D

A

H

H

A

G

A

A

S

R

L

L

F

F

G

G

L

L

Q

K

A

E

F

L

G

G

N

N

I

I

Y

Y

S

S

R

R

I

L

G

M

N

N

P

P

T

T

N

V

A

D

V

V

L

F

E

E

E

K

R

R

V

I

A

A

A

A

L

L

E

E

G

G

G

G

T

I

A

A

A

A

L

A

A

A

V

T

A

S

S
|
S

G
|
G

H
x
Q

A

A

A

A

Q
|
Q

F

F

L

L

V

T

M

I

H

A

A

T

L

L

L

A

Q

K

P

A

G

G

D

D

E

N

F

I

I

V

A

A

A

S

N

S

K

H

L

L

Y
|
Y

G

G

G

G

S

T

I

Y

N

N

Q

Q

F

L

N

N

H

V

S

L

Y

L

K

P

N

R

F

F

G

G

W

I

S

K

V

T

A

K

W

F

A

V

D

R

T

S

D

G

D

K

P

L

A

E

S

D

F

Y

E

A

A

A

A

A

I

I

T

D

P

D

R

Q

T

T

K

R

A

A

I

I

F

Y

V

V

E

E

S

S

I

M

A

S

N
|
N

P

P

G

D

G

Y

V

V

I

V

T

P

D

D

L

F

A

E

A

G

I

I

A

A

A

K

V

I

A

A

K

H

R

E

H

H

N

G

I

I

P

P

F

L

I

V

V

V

D
|
D

N

N

T

T

M

L

-

G

A

A

T

G

P

G

Y

Y

L

Y

I

I

R

R

P

P

F

I

E

E

H

H

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

A

T
|
T

K
|
K

F

W

L

I

G

G

G

G

H

H

G

G

N

T

S

T

I

I

G

G

G

G

L

V

I

I

V

V

D

D

G

S

G

G

T

R

F

F

A

N

W

W

A

N

G

K

-

H

D

S

D

D

R

R

Y

F

P

P

M

E

L

M

S

V

K

E

P

P

R

S

P

P

E

S

Y

Y

G

H

G

G

M

L

V

K

L

Y

S

W

E

E

T

A

F

F

G

G

N

P

F

A

G

T

F

F

A

I

I

T

A

R

V

I

R

R

V

V

L

E

G

M

L

L

R

R

D

D

L

I

G

G

P

A

A

C

L

L

S

S

P

P

F

F

N

S

A

A

F

Q

L

Q

I

L

L

L

N

L

G

G

I

I

E

E

T

T

L

L

P

G

L

L

R

R

M

A

Q

E

R

R

H

H

S

A

D

Q

N

N

A

T

R

E

V

K

V

L

A

S

Q

K

H

Y

L

F

E

E

R

S

H

S

P

P

E

N

V

V

S

S

W

W

V

V

S

L

Y

W

P

P

G

G

L

S

E

E

T

S

D

H

R

P

Y

T

H

Y

A

S

L

Q

H

A

K

K

R

K

Y

Y

C

L

P

T

L

R

G

G

A

F

G

G

A

A

V

M

F

L

T

S

F

I

G

G

L

V

K

K

G

G

G

D

Y

A

E

S

A

A

G

G

V

S

K

K

L

V

V

V

S

D

N

G

L

L

K

K

L

L

F

V

S

S

H

N

L

L

A

A

N

N

I

V

G

G

D

D

T

A

R

K

S

S

L

L

V

A

I

I

H

H

P

P

A

W

S

S

T

T

T

T

H

H

R

E

Q

Q

L

L

T

S

D

E

E

D

Q

E

K

R

V

L

R

A

A

S

G

G

A

V

G

T

P

E

E

D

V

M

V

I

R
|
R

L

I

S

S

I

V

G

G

I

I

E

E

D

H

P

V

A

D

D

D

L

I

I

I

A

A

D

D

L

F

D

E

Q

Q

A

S

L

F

binding (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid: S83 (= S86), G84 (= G87), Q85 (≠ H88), Q88 (= Q91), Y109 (= Y112), N156 (= N159), D181 (= D184), S204 (= S206), T206 (= T208), K207 (= K209), R404 (= R405)

8erbK Crystal structure of fub7 in complex with vinylglycine ketimine (see paper)
48% identity, 98% coverage: 8:424/426 of query aligns to 6:424/429 of 8erbK

query
sites
8erbK

F

F

D

E

T

T

L

L

A

Q

I

L

H

H

A

A

G

G

A

Y

A

T

P

P

D

D

A

P

A

H

T

T

G

R

A

S

R

T

A

A

T

V

P

P

I

I

Y

Y

Q

A

T

T

T

S

S

S

F

Y

V

T

F

F

D

N

D

D

V

S

D

A

H

H

A

G

A

A

S

R

L

L

F

F

G

G

L

L

Q

K

A

E

F

L

G

G

N

N

I

I

Y
|
Y

S

S

R
|
R

I

L

G

M

N

N

P

P

T

T

N

V

A

D

V

V

L

F

E

E

E

K

R

R

V

I

A

A

A

A

L

L

E

E

G

G

G

G

T

I

A

A

A

A

L

A

A

A

V

T

A

S

S

S

G
|
G

H
x
Q

A

A

A

A

Q
|
Q

F

F

L

L

V

T

M

I

H

A

A

T

L

L

L

A

Q

K

P

A

G

G

D

D

E

N

F

I

I

V

A

A

A

S

N

S

K

H

L

L

Y
|
Y

G

G

G

G

S

T

I

Y

N

N

Q

Q

F

L

N

N

H

V

S

L

Y

L

K

P

N

R

F

F

G

G

W

I

S

K

V

T

A

K

W

F

A

V

D

R

T

S

D

G

D

K

P

L

A

E

S

D

F

Y

E

A

A

A

A

A

I

I

T

D

P

D

R

Q

T

T

K

R

A

A

I

I

F

Y

V

V

E

E

S

S

I

M

A

S

N
|
N

P

P

G

D

G

Y

V

V

I

V

T

P

D

D

L

F

A

E

A

G

I

I

A

A

A

K

V

I

A

A

K

H

R

E

H

H

N

G

I

I

P

P

F

L

I

V

V

V

D
|
D

N

N

T

T

M

L

-

G

A

A

T

G

P

G

Y

Y

L

Y

I

I

R

R

P

P

F

I

E

E

H

H

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

A

T
|
T

K
|
K

F

W

L

I

G

G

G

G

H

H

G

G

N

T

S

T

I

I

G

G

G

G

L

V

I

I

V

V

D

D

G

S

G

G

T

R

F

F

A

N

W

W

A

N

G

K

-

H

D

S

D

D

R

R

Y

F

P

P

M

E

L

M

S

V

K

E

P

P

R

S

P

P

E

S

Y

Y

G

H

G

G

M

L

V

K

L

Y

S

W

E

E

T

A

F

F

G

G

N

P

F

A

G

T

F

F

A

I

I

T

A

R

V

I

R

R

V

V

L

E

G

M

L

L

R

R

D

D

L

I

G

G

P

A

A

C

L

L

S

S

P

P

F

F

N

S

A

A

F

Q

L

Q

I

L

L

L

N

L

G

G

I

I

E

E

T

T

L

L

P

G

L

L

R

R

M

A

Q

E

R

R

H

H

S

A

D

Q

N

N

A

T

R

E

V

K

V

L

A

S

Q

K

H

Y

L

F

E

E

R

S

H

S

P

P

E

N

V

V

S

S

W

W

V

V

S

L

Y

W

P

P

G

G

L

S

E

E

T

S

D

H

R

P

Y

T

H

Y

A

S

L

Q

H

A

K

K

R

K

Y

Y

C

L

P

T

L

R

G

G

A

F

G

G

A

A

V

M

F

L

T

S

F

I

G

G

L

V

K

K

G

G

G

D

Y

A

E

S

A

A

G

G

V

S

K

K

L

V

V

V

S

D

N

G

L

L

K

K

L

L

F

V

S

S

H

N

L

L

A

A

N

N

I

V

G

G

D

D

T

A

R

K

S

S

L

L

V

A

I

I

H

H

P

P

A

W

S

S

T

T

T
|
T

H

H

R

E

Q

Q

L

L

T

S

D

E

E

D

Q

E

K

R

V

L

R

A

A

S

G

G

A

V

G

T

P

E

E

D

V

M

V

I

R
|
R

L

I

S

S

I

V

G

G

I

I

E

E

D

H

P

V

A

D

D

D

L

I

I

I

A

A

D

D

L

F

D

E

Q

Q

A

S

L

F

binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: Y55 (= Y57), R57 (= R59), G85 (= G87), Q86 (≠ H88), Q89 (= Q91), Y110 (= Y112), N157 (= N159), D182 (= D184), S205 (= S206), T207 (= T208), K208 (= K209), T385 (= T385), R405 (= R405)

4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
39% identity, 99% coverage: 4:424/426 of query aligns to 4:392/396 of 4omaA

query
sites
4omaA

R

R

L

T

P

Y

G

G

F

F

D

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

A

Q

P

P

D

D

A

P

A

S

T

T

G

G

A

A

R

L

A

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

F

F

V

V

F

F

D

D

D

S

V

A

D

E

H

Q

A

G

A

A

S

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

S

T

R
|
R

I

L

G

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

H
x
I

A

S

A

A

Q

I

F

T

L

T

V

T

M

L

H

L

A

T

L

L

L

C

Q

Q

P

Q

G

G

D

D

E

H

F

I

I

V

A

S

A

A

N

S

K

A

L

I

Y
|
Y

G

G

G

C

S

T

I

H

N

A

Q

F

F

L

N

S

H

H

S

S

Y

M

K

P

N

K

F

F

G

G

W

I

S

N

V

V

A

S

W

F

A

V

D

D

T

A

D

A

D

K

P

P

A

E

S

E

F

I

E

R

A

A

A

A

I

M

T

R

P

P

R

E

T

T

K

K

A

V

I

V

F

Y

V

I

E

E

S

T

I

P

A

A

N

N

P

P

G

T

G

L

V

S

I

L

T

V

D

D

L

I

A

E

A

T

I

V

A

A

A

G

V

I

A

A

K

H

R

Q

H

Q

N

G

I

A

P

L

F

L

I

V

V

V

D
|
D

N

N

T

T

M

F

A

M

T

S

P

P

Y

Y

L

C

I

Q

R

Q

P

P

F

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

V

T
|
T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

I

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

A

-

W

-

A

-

G

-

D

-

D

-

R

-

Y

-

P

-

M

-

L

-

S

-

K

-

P

-

R

-

P

-

E

-

Y

-

G

-

G

-

M

-

V

-

L

-

S

-

E

-

T

-

F

-

G

G

N

K

F

Q

G

E

F

F

A

I

I

D

A

Q

V

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

N

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

R

L

V

K

V

I

A

A

Q

R

H

F

L

L

E

E

R

G

H

H

P

P

E

S

V

I

S

T

W

R

V

V

S

Y

Y

Y

P

P

G

G

L

L

E

S

T

S

D

H

R

P

Y

Q

H

Y

A

E

L

L

H

G

K

Q

R

R

Y

Q

C

M

P

S

L

L

G

-

A

P

G

G

A

G

V

I

F

I

T

S

F

F

G

E

L

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

V

R

K

R

L

M

V

I

S

N

N

S

L

V

K

E

L

L

F

C

S

L

H

L

L

A

A
x
V

N
x
S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

T

A

D

P

E

E

Q

E

K

R

V

L

R

K

A

A

G

G

A

I

G

T

P

D

E

G

V

L

V

I

R
|
R

L

L

S

S

I

V

G

G

I

L

E

E

D

D

P

P

A

E

D

D

L

I

I

I

A

N

D

D

L

L

D

E

Q

H

A

A

L

I

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G87), I88 (≠ H88), Y112 (= Y112), D184 (= D184), S206 (= S206), T208 (= T208), K209 (= K209), V337 (≠ A369), S338 (≠ N370), R373 (= R405)

3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
39% identity, 99% coverage: 4:424/426 of query aligns to 4:392/396 of 3jwbA

query
sites
3jwbA

R

R

L

T

P

Y

G

G

F

F

D

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

A

Q

P

P

D

D

A

P

A

S

T

T

G

G

A

A

R

L

A

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

F

F

V

V

F

F

D

D

D

S

V

A

D

E

H

Q

A

G

A

A

S

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

S

T

R
|
R

I

L

G

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G

G

H

I

A

S

A

A

Q

I

F

T

L

T

V

T

M

L

H

L

A

T

L

L

L

C

Q

Q

P

Q

G

G

D

D

E

H

F

I

I

V

A

S

A

A

N

S

K

A

L

I

Y
|
Y

G

G

G

C

S

T

I

H

N

A

Q

F

F

L

N

S

H

H

S

S

Y

M

K

P

N

K

F

F

G

G

W

I

S

N

V

V

A

S

W

F

A

V

D

D

T

A

D

A

D

K

P

P

A

E

S

E

F

I

E

R

A

A

A

A

I

M

T

R

P

P

R

E

T

T

K

K

A

V

I

V

F

Y

V

I

E

E

S

T

I

P

A

A

N

N

P

P

G

T

G

L

V

S

I

L

T

V

D

D

L

I

A

E

A

T

I

V

A

A

A

G

V

I

A

A

K

H

R

Q

H

Q

N

G

I

A

P

L

F

L

I

V

V

V

D
|
D

N

N

T

T

M

F

A

M

T

S

P

P

Y

Y

L

C

I

Q

R

Q

P

P

F

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S

S

A

V

T

T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

I

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

A

-

W

-

A

-

G

-

D

-

D

-

R

-

Y

-

P

-

M

-

L

-

S

-

K

-

P

-

R

-

P

-

E

-

Y

-

G

-

G

-

M

-

V

-

L

-

S

-

E

-

T

-

F

-

G

G

N

K

F

Q

G

E

F

F

A

I

I

D

A

Q

V

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

N

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

R

L

V

K

V

I

A

A

Q

R

H

F

L

L

E

E

R

G

H

H

P

P

E

S

V

I

S

T

W

R

V

V

S

Y

Y

Y

P

P

G

G

L

L

E

S

T

S

D

H

R

P

Y

Q

H

Y

A

E

L

L

H

G

K

Q

R

R

Y

Q

C

M

P

S

L

L

G

-

A

P

G

G

A

G

V

I

F

I

T

S

F

F

G

E

L

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

V

R

K

R

L

M

V

I

S

N

N

S

L

V

K

E

L

L

F

C

S

L

H

L

L

A

A

V

N
x
S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

T

A

D

P

E

E

Q

E

K

R

V

L

R

K

A

A

G

G

A

I

G

T

P

D

E

G

V

L

V

I

R
|
R

L

L

S

S

I

V

G

G

I

L

E

E

D

D

P

P

A

E

D

D

L

I

I

I

A

N

D

D

L

L

D

E

Q

H

A

A

L

I

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding norleucine: Y112 (= Y112), K209 (= K209), S338 (≠ N370), R373 (= R405)

3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
39% identity, 99% coverage: 4:424/426 of query aligns to 4:392/396 of 3jwaA

query
sites
3jwaA

R

R

L

T

P

Y

G

G

F

F

D

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

A

Q

P

P

D

D

A

P

A

S

T

T

G

G

A

A

R

L

A

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

F

F

V

V

F

F

D

D

D

S

V

A

D

E

H

Q

A

G

A

A

S

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

S

T

R
|
R

I

L

G

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G

G

H

I

A

S

A

A

Q

I

F

T

L

T

V

T

M

L

H

L

A

T

L

L

L

C

Q

Q

P

Q

G

G

D

D

E

H

F

I

I

V

A

S

A

A

N

S

K

A

L

I

Y
|
Y

G

G

G

C

S

T

I

H

N

A

Q

F

F

L

N

S

H

H

S

S

Y

M

K

P

N

K

F

F

G

G

W

I

S

N

V

V

A

S

W

F

A

V

D

D

T

A

D

A

D

K

P

P

A

E

S

E

F

I

E

R

A

A

A

A

I

M

T

R

P

P

R

E

T

T

K

K

A

V

I

V

F

Y

V

I

E

E

S

T

I

P

A

A

N

N

P

P

G

T

G

L

V

S

I

L

T

V

D

D

L

I

A

E

A

T

I

V

A

A

A

G

V

I

A

A

K

H

R

Q

H

Q

N

G

I

A

P

L

F

L

I

V

V

V

D
|
D

N

N

T

T

M

F

A

M

T

S

P

P

Y

Y

L

C

I

Q

R

Q

P

P

F

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S

S

A

V

T

T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

I

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

A

-

W

-

A

-

G

-

D

-

D

-

R

-

Y

-

P

-

M

-

L

-

S

-

K

-

P

-

R

-

P

-

E

-

Y

-

G

-

G

-

M

-

V

-

L

-

S

-

E

-

T

-

F

-

G

G

N

K

F

Q

G

E

F

F

A

I

I

D

A

Q

V

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

N

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

R

L

V

K

V

I

A

A

Q

R

H

F

L

L

E

E

R

G

H

H

P

P

E

S

V

I

S

T

W

R

V

V

S

Y

Y

Y

P

P

G

G

L

L

E

S

T

S

D

H

R

P

Y

Q

H

Y

A

E

L

L

H

G

K

Q

R

R

Y

Q

C

M

P

S

L

L

G

-

A

P

G

G

A

G

V

I

F

I

T

S

F

F

G

E

L

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

V

R

K

R

L

M

V

I

S

N

N

S

L

V

K

E

L

L

F

C

S

L

H

L

L

A

A
x
V

N
x
S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

T

A

D

P

E

E

Q

E

K

R

V

L

R

K

A

A

G

G

A

I

G

T

P

D

E

G

V

L

V

I

R
|
R

L

L

S

S

I

V

G

G

I

L

E

E

D

D

P

P

A

E

D

D

L

I

I

I

A

N

D

D

L

L

D

E

Q

H

A

A

L

I

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding (1-amino-3-methylsulfanyl-propyl)-phosphinic acid: Y112 (= Y112), K209 (= K209), V337 (≠ A369), S338 (≠ N370), R373 (= R405)

3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
39% identity, 99% coverage: 4:424/426 of query aligns to 4:392/396 of 3jw9A

query
sites
3jw9A

R

R

L

T

P

Y

G

G

F

F

D

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

A

Q

P

P

D

D

A

P

A

S

T

T

G

G

A

A

R

L

A

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

F

F

V

V

F

F

D

D

D

S

V

A

D

E

H

Q

A

G

A

A

S

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

S

T

R
|
R

I

L

G

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G

G

H

I

A

S

A

A

Q

I

F

T

L

T

V

T

M

L

H

L

A

T

L

L

L

C

Q

Q

P

Q

G

G

D

D

E

H

F

I

I

V

A

S

A

A

N

S

K

A

L

I

Y
|
Y

G

G

G

C

S

T

I

H

N

A

Q

F

F

L

N

S

H

H

S

S

Y

M

K

P

N

K

F

F

G

G

W

I

S

N

V

V

A

S

W

F

A

V

D

D

T

A

D

A

D

K

P

P

A

E

S

E

F

I

E

R

A

A

A

A

I

M

T

R

P

P

R

E

T

T

K

K

A

V

I

V

F

Y

V

I

E

E

S

T

I

P

A

A

N

N

P

P

G

T

G

L

V

S

I

L

T

V

D

D

L

I

A

E

A

T

I

V

A

A

A

G

V

I

A

A

K

H

R

Q

H

Q

N

G

I

A

P

L

F

L

I

V

V

V

D
|
D

N

N

T

T

M

F

A

M

T

S

P

P

Y

Y

L

C

I

Q

R

Q

P

P

F

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S

S

A

V

T

T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

I

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

A

-

W

-

A

-

G

-

D

-

D

-

R

-

Y

-

P

-

M

-

L

-

S

-

K

-

P

-

R

-

P

-

E

-

Y

-

G

-

G

-

M

-

V

-

L

-

S

-

E

-

T

-

F

-

G

G

N

K

F

Q

G

E

F

F

A

I

I

D

A

Q

V

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

N

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

R

L

V

K

V

I

A

A

Q

R

H

F

L

L

E

E

R

G

H

H

P

P

E

S

V

I

S

T

W

R

V

V

S

Y

Y

Y

P

P

G

G

L

L

E

S

T

S

D

H

R

P

Y

Q

H

Y

A

E

L

L

H

G

K

Q

R

R

Y

Q

C

M

P

S

L

L

G

-

A

P

G

G

A

G

V

I

F

I

T

S

F

F

G

E

L

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

V

R

K

R

L

M

V

I

S

N

N

S

L

V

K

E

L

L

F

C

S

L

H

L

L

A

A
x
V

N
x
S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

T

A

D

P

E

E

Q

E

K

R

V

L

R

K

A

A

G

G

A

I

G

T

P

D

E

G

V

L

V

I

R

R

L

L

S

S

I

V

G

G

I

L

E

E

D

D

P

P

A

E

D

D

L

I

I

I

A

N

D

D

L

L

D

E

Q

H

A

A

L

I

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding S-ethyl-L-cysteine: Y112 (= Y112), K209 (= K209), V337 (≠ A369), S338 (≠ N370)

5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
39% identity, 99% coverage: 4:424/426 of query aligns to 3:391/395 of 5m3zA

query
sites
5m3zA

R

R

L

T

P

Y

G

G

F

F

D

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

A

Q

P

P

D

D

A

P

A

S

T

T

G

G

A

A

R

L

A

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

F

F

V

V

F

F

D

D

D

S

V

A

D

E

H

Q

A

G

A

A

S

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

S

T

R
|
R

I

L

G

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

H
x
I

A

S

A

A

Q

I

F

T

L

T

V

T

M

L

H

L

A

T

L

L

L

C

Q

Q

P

Q

G

G

D

D

E

H

F

I

I

V

A

S

A

A

N

S

K

A

L

I

Y
|
Y

G

G

G
x
H

S

T

I

H

N

A

Q

F

F

L

N

S

H

H

S

S

Y

M

K

P

N

K

F

F

G

G

W

I

S

N

V

V

A

S

W

F

A

V

D

D

T

A

D

A

D

K

P

P

A

E

S

E

F

I

E

R

A

A

A

A

I

M

T

R

P

P

R

E

T

T

K

K

A

V

I

V

F

Y

V

I

E
|
E

S

T

I

P

A

A

N

N

P

P

G

T

G

L

V

S

I

L

T

V

D

D

L

I

A

E

A

T

I

V

A

A

A

G

V

I

A

A

K

H

R

Q

H

Q

N

G

I

A

P

L

F

L

I

V

V

V

D
|
D

N

N

T
|
T

M

F

A

M

T

S

P

P

Y

Y

L

C

I

Q

R

Q

P

P

F

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

V

T
|
T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

I

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

A

-

W

-

A

-

G

-

D

-

D

-

R

-

Y

-

P

-

M

-

L

-

S

-

K

-

P

-

R

-

P

-

E

-

Y

-

G

-

G

-

M

-

V

-

L

-

S

-

E

-

T

-

F

-

G

G

N

K

F

Q

G

E

F

F

A

I

I

D

A

Q

V

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

N

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

R

L

V

K

V

I

A

A

Q

R

H

F

L

L

E

E

R

G

H

H

P

P

E

S

V

I

S

T

W

R

V

V

S

Y

Y

Y

P

P

G

G

L

L

E

S

T

S

D

H

R

P

Y

Q

H

Y

A

E

L

L

H

G

K

Q

R

R

Y

Q

C

M

P

S

L

L

G

-

A

P

G

G

A

G

V

I

F

I

T

S

F

F

G

E

L

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

V

R

K

R

L

M

V

I

S

N

N

S

L

V

K

E

L

L

F

C

S

L

H

L

L

A

A
x
V

N
x
S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

T

A

D

P

E

E

Q

E

K

R

V

L

R

K

A

A

G

G

A

I

G

T

P

D

E

G

V

L

V

I

R
|
R

L

L

S

S

I

V

G

G

I

L

E

E

D

D

P

P

A

E

D

D

L

I

I

I

A

N

D

D

L

L

D

E

Q

H

A

A

L

I

active site: R58 (= R59), Y111 (= Y112), D183 (= D184), K208 (= K209)
binding norleucine: Y111 (= Y112), H113 (≠ G114), K208 (= K209), V336 (≠ A369), S337 (≠ N370)
binding pyridoxal-5'-phosphate: G86 (= G87), I87 (≠ H88), Y111 (= Y112), E154 (= E155), D183 (= D184), T185 (= T186), S205 (= S206), T207 (= T208), K208 (= K209)
binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G87), I87 (≠ H88), Y111 (= Y112), D183 (= D184), S205 (= S206), T207 (= T208), K208 (= K209), V336 (≠ A369), S337 (≠ N370), R372 (= R405)

6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
39% identity, 99% coverage: 4:424/426 of query aligns to 4:392/396 of 6egrA

query
sites
6egrA

R

R

L

T

P

Y

G

G

F

F

D

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

A

Q

P

P

D

D

A

P

A

S

T

T

G

G

A

A

R

L

A

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

F

F

V

V

F

F

D

D

D

S

V

A

D

E

H

Q

A

G

A

A

S

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

S

T

R

R

I

L

G

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

H
x
I

A

S

A

A

Q

I

F

T

L

T

V

T

M

L

H

L

A

T

L

L

L

C

Q

Q

P

Q

G

G

D

D

E

H

F

I

I

V

A

S

A

A

N

S

K

A

L

I

Y
|
Y

G

G

G

C

S

T

I

H

N

A

Q

F

F

L

N

S

H

H

S

S

Y

M

K

P

N

K

F

F

G

G

W

I

S

N

V

V

A

S

W

F

A

V

D

D

T

A

D

A

D

K

P

P

A

E

S

E

F

I

E

R

A

A

A

A

I

M

T

R

P

P

R

E

T

T

K

K

A

V

I

V

F

Y

V

I

E
|
E

S

T

I

P

A

A

N

N

P

P

G

T

G

L

V

S

I

L

T

V

D

D

L

I

A

E

A

T

I

V

A

A

A

G

V

I

A

A

K

H

R

Q

H

Q

N

G

I

A

P

L

F

L

I

V

V

V

D
|
D

N

N

T
|
T

M
x
F

A

M

T

S

P

P

Y

Y

L

C

I

Q

R

Q

P

P

F

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

V

T
|
T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

I

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

A

-

W

-

A

-

G

-

D

-

D

-

R

-

Y

-

P

-

M

-

L

-

S

-

K

-

P

-

R

-

P

-

E

-

Y

-

G

-

G

-

M

-

V

-

L

-

S

-

E

-

T

-

F

-

G

G

N

K

F

Q

G

E

F

F

A

I

I

D

A

Q

V

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

N

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

R

L

V

K

V

I

A

A

Q

R

H

F

L

L

E

E

R

G

H

H

P

P

E

S

V

I

S

T

W

R

V

V

S

Y

Y

Y

P

P

G

G

L

L

E

S

T

S

D

H

R

P

Y

Q

H

Y

A

E

L

L

H

G

K

Q

R

R

Y

Q

C

M

P

S

L

L

G

-

A

P

G

G

A

G

V

I

F

I

T

S

F

F

G

E

L

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

V

R

K

R

L

M

V

I

S

N

N

S

L

V

K

E

L

L

F

C

S

L

H

L

L

A

A

V

N

S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

Y

T

A

D

P

E

E

Q

E

K

R

V

L

R

K

A

A

G

G

A

I

G

T

P

D

E

G

V

L

V

I

R

R

L

L

S

S

I

V

G

G

I

L

E

E

D

D

P

P

A

E

D

D

L

I

I

I

A

N

D

D

L

L

D

E

Q

H

A

A

L

I

binding pyridoxal-5'-phosphate: G87 (= G87), I88 (≠ H88), Y112 (= Y112), E155 (= E155), D184 (= D184), T186 (= T186), F187 (≠ M187), S206 (= S206), T208 (= T208), K209 (= K209)

4hf8A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with glycine (see paper)
39% identity, 99% coverage: 4:424/426 of query aligns to 4:392/396 of 4hf8A

query
sites
4hf8A

R

R

L

T

P

Y

G

G

F

F

D

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

A

Q

P

P

D

D

A

P

A

S

T

T

G

G

A

A

R

L

A

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

F

F

V

V

F

F

D

D

D

S

V

A

D

E

H

Q

A

G

A

A

S

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

S

T

R
|
R

I

L

G

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

H
x
I

A

S

A

A

Q

I

F

T

L

T

V

T

M

L

H

L

A

T

L

L

L

C

Q

Q

P

Q

G

G

D

D

E

H

F

I

I

V

A

S

A

A

N

S

K

A

L

I

Y
|
Y

G

G

G

C

S

T

I

H

N

A

Q

F

F

L

N

S

H

H

S

S

Y

M

K

P

N

K

F

F

G

G

W

I

S

N

V

V

A

R

W

F

A

V

D

D

T

A

D

A

D

K

P

P

A

E

S

E

F

I

E

R

A

A

A

A

I

M

T

R

P

P

R

E

T

T

K

K

A

V

I

V

F

Y

V

I

E
|
E

S

T

I

P

A

A

N
|
N

P

P

G

T

G

L

V

S

I

L

T

V

D

D

L

I

A

E

A

T

I

V

A

A

A

G

V

I

A

A

K

H

R

Q

H

Q

N

G

I

A

P

L

F

L

I

V

V

V

D
|
D

N

N

T

T

M

F

A

M

T

S

P

P

Y

Y

L

C

I

Q

R

Q

P

P

F

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

V

T

T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

I

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

A

-

W

-

A

-

G

-

D

-

D

-

R

-

Y

-

P

-

M

-

L

-

S

-

K

-

P

-

R

-

P

-

E

-

Y

-

G

-

G

-

M

-

V

-

L

-

S

-

E

-

T

-

F

-

G

G

N

K

F

Q

G

E

F

F

A

I

I

D

A

Q

V

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

N

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

R

L

V

K

V

I

A

A

Q

R

H

F

L

L

E

E

R

G

H

H

P

P

E

S

V

I

S

T

W

R

V

V

S

Y

Y

Y

P

P

G

G

L

L

E

S

T

S

D

H

R

P

Y

Q

H

Y

A

E

L

L

H

G

K

Q

R

R

Y

Q

C

M

P

S

L

L

G

-

A

P

G

G

A

G

V

I

F

I

T

S

F

F

G

E

L

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

V

R

K

R

L

M

V

I

S

N

N

S

L

V

K

E

L

L

F

C

S

L

H

L

L

A

A

V

N
x
S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

T

A

D

P

E

E

Q

E

K

R

V

L

R

K

A

A

G

G

A

I

G

T

P

D

E

G

V

L

V

I

R
|
R

L

L

S

S

I

V

G

G

I

L

E

E

D

D

P

P

A

E

D

D

L

I

I

I

A

N

D

D

L

L

D

E

Q

H

A

A

L

I

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: G87 (= G87), I88 (≠ H88), Y112 (= Y112), E155 (= E155), N159 (= N159), D184 (= D184), S206 (= S206), K209 (= K209), S338 (≠ N370), R373 (= R405)

1pg8A Crystal structure of l-methionine alpha-, gamma-lyase
41% identity, 99% coverage: 2:424/426 of query aligns to 4:394/398 of 1pg8A

query
sites
1pg8A

T

S

D

N

R

K

L

L

P

P

G

G

F

F

D

A

T

T

L

R

A

A

I

I

H

H

A

H

G

G

A

Y

A

D

P

P

D

Q

A

D

A

H

T

G

G

G

A

A

R

L

A

V

T

P

P

P

I

V

Y

Y

Q

Q

T

T

T

A

S

T

F

F

V

T

F

F

D

P

D

T

V

V

D

E

H

Y

A

G

A

A

S

A

L

C

F

F

G

A

L

G

Q

E

A

Q

F

A

G

G

N

H

I

F

Y
|
Y

S

S

R
|
R

I

I

G

S

N

N

P

P

T

T

N

L

A

N

V

L

L

L

E

E

E

A

R

R

V

M

A

A

A

S

L

L

E

E

G

G

G

G

T

E

A

A

A

G

L

L

A

A

V

L

A

A

S
|
S

G
|
G

H
x
M

A

G

A

A

Q

I

F

T

L

S

V

T

M

L

H

W

A

T

L

L

L

L

Q

R

P

P

G

G

D

D

E

E

F

V

I

L

A

L

A

G

N

N

K

T

L

L

Y
|
Y

G

G

G

C

S

T

I

F

N

A

Q

F

F

L

N

H

H

H

S

G

Y

I

K

G

N

E

F

F

G

G

W

V

S

K

V

L

A

R

W

H

A

V

D

D

T

M

D

A

D

D

P

L

A

Q

S

A

F

L

E

E

A

A

A

A

I

M

T

T

P

P

R

A

T

T

K

R

A

V

I

I

F

Y

V

F

E

E

S

S

I

P

A

A

N

N

P

P

G

N

G

M

V

H

I

M

T

A

D

D

L

I

A

A

A

G

I

V

A

A

A

K

V

I

A

A

K

R

R

K

H

H

N

G

I

A

P

T

F

V

I

V

V

V

D
|
D

N

N

T

T

M

Y

A

C

T

T

P

P

Y

Y

L

L

I

Q

R

R

P

P

F

L

E

E

H

L

G

G

A

A

D

D

I

L

V

V

V

V

H

H

S
|
S

A

A

T
|
T

K
|
K

F

Y

L

L

G

S

G

G

H

H

G

G

N

D

S

I

I

T

G

A

G

G

L

I

I

V

V

V

D

-

G

-

G

-

T

-

F

-

A

-

W

-

A

-

G

-

D

-

D

-

R

-

Y

-

P

-

M

-

L

-

S

-

K

-

P

-

R

-

P

-

E

-

Y

-

G

-

G

-

M

-

V

-

L

-

S

-

E

-

T

-

F

-

G

G

N

S

F

Q

G

A

F

L

A

V

I

D

A

R

V

I

R

R

V

L

L

Q

G

G

L

L

R

K

D

D

L

M

-

T

G

G

P

A

A

V

L

L

S

S

P

P

F

H

N

D

A

A

F

A

L

L

I

L

L

M

N

R

G

G

I

I

E

K

T

T

L

L

P

N

L

L

R

R

M

M

Q

D

R

R

H

H

S

C

D

A

N

N

A

A

R

Q

V

V

V

L

A

A

Q

E

H

F

L

L

E

A

R

R

H

Q

P

P

E

Q

V

V

S

E

W

L

V

I

S

H

Y

Y

P

P

G

G

L

L

E

A

T

S

D

F

R

P

Y

Q

H

Y

A

T

L

L

H

A

K

R

R

Q

Y

Q

C

M

P

S

L

-

G

Q

A

P

G

G

A

G

V

M

F

I

T

A

F

F

G

E

L

L

K

K

G

G

G

G

Y

I

E

G

A

A

G

G

V

R

K

R

L

F

V

M

S

N

N

A

L

L

K

Q

L

L

F

F

S

S

H

R

L

A

A

V

N

S

I

L

G

G

D

D

T

A

R

E

S

S

L

L

V

A

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

S

L

Y

T

T

D

P

E

E

Q

E

K

R

V

A

R

H

A

Y

G

G

A

I

G

S

P

E

E

G

V

L

V

V

R

R

L

L

S

S

I

V

G

G

I

L

E

E

D

D

P

I

A

D

D

D

L

L

I

L

A

A

D

D

L

V

D

Q

Q

Q

A

A

L

L

active site: R61 (= R59), Y114 (= Y112), D186 (= D184), K211 (= K209)
binding pyridoxal-5'-phosphate: Y59 (= Y57), R61 (= R59), S88 (= S86), G89 (= G87), M90 (≠ H88), Y114 (= Y112), D186 (= D184), S208 (= S206), T210 (= T208), K211 (= K209)

P13254 L-methionine gamma-lyase; MGL; Homocysteine desulfhydrase; L-methioninase; EC 4.4.1.11; EC 4.4.1.2 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
41% identity, 99% coverage: 2:424/426 of query aligns to 4:394/398 of P13254

query
sites
P13254

T

S

D

N

R

K

L

L

P

P

G

G

F

F

D

A

T

T

L

R

A

A

I

I

H

H

A

H

G

G

A

Y

A

D

P

P

D

Q

A

D

A

H

T

G

G

G

A

A

R

L

A

V

T

P

P

P

I

V

Y

Y

Q

Q

T

T

T

A

S

T

F

F

V

T

F

F

D

P

D

T

V

V

D

E

H

Y

A

G

A

A

S

A

L

C

F

F

G

A

L

G

Q

E

A

Q

F

A

G

G

N

H

I

F

Y
|
Y

S
|
S

R
|
R

I

I

G

S

N

N

P

P

T

T

N

L

A

N

V

L

L

L

E

E

E

A

R

R

V

M

A

A

A

S

L

L

E

E

G

G

G

G

T

E

A

A

A

G

L

L

A

A

V

L

A

A

S

S

G
|
G

H
x
M

A

G

A

A

Q

I

F

T

L

S

V

T

M

L

H

W

A

T

L

L

L

L

Q

R

P

P

G

G

D

D

E

E

F

V

I

L

A

L

A

G

N

N

K

T

L

L

Y
|
Y

G

G

G
x
C

S

T

I

F

N

A

Q

F

F

L

N

H

H

H

S

G

Y

I

K

G

N

E

F

F

G

G

W

V

S

K

V

L

A

R

W

H

A

V

D

D

T

M

D

A

D

D

P

L

A

Q

S

A

F

L

E

E

A

A

A

A

I

M

T

T

P

P

R

A

T

T

K

R

A

V

I

I

F

Y

V

F

E

E

S

S

I

P

A

A

N

N

P

P

G

N

G

M

V

H

I

M

T

A

D

D

L

I

A

A

A

G

I

V

A

A

A

K

V

I

A

A

K

R

R

K

H

H

N

G

I

A

P

T

F

V

I

V

V

V

D

D

N

N

T

T

M

Y

A

C

T

T

P

P

Y

Y

L

L

I

Q

R

R

P

P

F

L

E

E

H

L

G

G

A

A

D

D

I

L

V

V

V

V

H

H

S
|
S

A
|
A

T
|
T

K
|
K

F

Y

L

L

G

S

G

G

H

H

G

G

N

D

S

I

I

T

G

A

G

G

L

I

I

V

V

V

D

-

G

-

G

-

T

-

F

-

A

-

W

-

A

-

G

-

D

-

D

-

R

-

Y

-

P

-

M

-

L

-

S

-

K

-

P

-

R

-

P

-

E

-

Y

-

G

-

G

-

M

-

V

-

L

-

S

-

E

-

T

-

F

-

G

G

N

S

F

Q

G

A

F

L

A

V

I

D

A

R

V

I

R

R

V

L

L

Q

G

G

L

L

R
x
K

D
|
D

L

M

-

T

G

G

P

A

A

V

L

L

S

S

P

P

F

H

N

D

A

A

F

A

L

L

I

L

L

M

N

R

G

G

I

I

E

K

T

T

L

L

P

N

L

L

R

R

M

M

Q

D

R

R

H

H

S

C

D

A

N

N

A

A

R

Q

V

V

V

L

A

A

Q

E

H

F

L

L

E

A

R

R

H

Q

P

P

E

Q

V

V

S

E

W

L

V

I

S

H

Y

Y

P

P

G

G

L

L

E

A

T

S

D

F

R

P

Y

Q

H

Y

A

T

L

L

H

A

K

R

R

Q

Y

Q

C

M

P

S

L

-

G

Q

A

P

G

G

A

G

V

M

F

I

T

A

F

F

G

E

L

L

K

K

G

G

G

G

Y

I

E

G

A

A

G

G

V

R

K

R

L

F

V

M

S

N

N

A

L

L

K

Q

L

L

F

F

S

S

H

R

L

A

A

V

N

S

I

L

G

G

D

D

T

A

R

E

S

S

L

L

V

A

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

S

L

Y

T

T

D

P

E

E

Q

E

K

R

V

A

R

H

A

Y

G

G

A

I

G

S

P

E

E

G

V

L

V

V

R
|
R

L

L

S

S

I

V

G

G

I

L

E

E

D

D

P

I

A

D

D

D

L

L

I

L

A

A

D

D

L

V

D

Q

Q

Q

A

A

L

L

YSR 59:61 (= YSR 57:59) binding pyridoxal 5'-phosphate
R61 (= R59) mutation R->A,E,F: Loss of elimination activity against L-methionine.
GM 89:90 (≠ GH 87:88) binding in other chain
Y114 (= Y112) binding substrate
C116 (≠ G114) mutation to H: Drastic decrease of the catalytic efficiency of the elimination reaction with L-methionine, by 6700-fold, and increases that with L-cysteine by 7-fold, mainly due to changes in kcat. Loss of ability to catalyze replacement reaction between L-methionine and 2-mercaptoethanol.; mutation to S: 9% of wild-type elimination activity against L-methionine.; mutation to T: 40% of wild-type elimination activity against L-methionine.
SAT 208:210 (= SAT 206:208) binding in other chain
K211 (= K209) modified: N6-(pyridoxal phosphate)lysine
K240 (≠ R270) mutation K->D,E: Marked decrease in elimination activity against both L-methionine and DL-homocysteine.; mutation to M: 50% reduction in alpha,gamma-elimination activity against DL-homocysteine, while retaining elimination activity against L-methionine and L-cysteine.
D241 (= D271) mutation D->H,R: 5 to 14-fold reduction in alpha,gamma-elimination activity against L-methionine, while no change in affinity for L-methionine.
R375 (= R405) binding substrate

3vk3A Crystal structure of l-methionine gamma-lyase from pseudomonas putida c116h mutant complexed with l-methionine (see paper)
41% identity, 99% coverage: 2:424/426 of query aligns to 3:393/397 of 3vk3A

query
sites
3vk3A

T

S

D

N

R

K

L

L

P

P

G

G

F

F

D

A

T

T

L

R

A

A

I

I

H

H

A

H

G

G

A

Y

A

D

P

P

D

Q

A

D

A

H

T

G

G

G

A

A

R

L

A

V

T

P

P

P

I

V

Y

Y

Q

Q

T

T

T

A

S

T

F

F

V

T

F

F

D

P

D

T

V

V

D

E

H

Y

A

G

A

A

S

A

L

C

F

F

G

A

L

G

Q

E

A

Q

F

A

G

G

N

H

I

F

Y

Y

S

S

R
|
R

I

I

G

S

N

N

P

P

T

T

N

L

A

N

V

L

L

L

E

E

E

A

R

R

V

M

A

A

A

S

L

L

E

E

G

G

G

G

T

E

A

A

A

G

L

L

A

A

V

L

A

A

S

S

G

G

H

M

A

G

A

A

Q

I

F

T

L

S

V

T

M

L

H

W

A

T

L

L

L

L

Q

R

P

P

G

G

D

D

E

E

F

V

I

L

A

L

A

G

N

N

K

T

L

L

Y
|
Y

G

G

G

H

S

T

I

F

N

A

Q

F

F

L

N

H

H

H

S

G

Y

I

K

G

N

E

F

F

G

G

W

V

S

K

V

L

A

R

W

H

A

V

D

D

T

M

D

A

D

D

P

L

A

Q

S

A

F

L

E

E

A

A

A

A

I

M

T

T

P

P

R

A

T

T

K

R

A

V

I

I

F

Y

V

F

E

E

S

S

I

P

A

A

N

N

P

P

G

N

G

M

V

H

I

M

T

A

D

D

L

I

A

A

A

G

I

V

A

A

A

K

V

I

A

A

K

R

R

K

H

H

N

G

I

A

P

T

F

V

I

V

V

V

D
|
D

N

N

T

T

M

Y

A

C

T

T

P

P

Y

Y

L

L

I

Q

R

R

P

P

F

L

E

E

H

L

G

G

A

A

D

D

I

L

V

V

V

V

H

H

S

S

A

A

T

T

K
|
K

F

Y

L

L

G

S

G

G

H

H

G

G

N

D

S

I

I

T

G

A

G

G

L

I

I

V

V

V

D

-

G

-

G

-

T

-

F

-

A

-

W

-

A

-

G

-

D

-

D

-

R

-

Y

-

P

-

M

-

L

-

S

-

K

-

P

-

R

-

P

-

E

-

Y

-

G

-

G

-

M

-

V

-

L

-

S

-

E

-

T

-

F

-

G

G

N

S

F

Q

G

A

F

L

A

V

I

D

A

R

V

I

R

R

V

L

L

Q

G

G

L

L

R

K

D

D

L

M

-

T

G

G

P

A

A

V

L

L

S

S

P

P

F

H

N

D

A

A

F

A

L

L

I

L

L

M

N

R

G

G

I

I

E

K

T

T

L

L

P

N

L

L

R

R

M

M

Q

D

R

R

H

H

S

C

D

A

N

N

A

A

R

Q

V

V

V

L

A

A

Q

E

H

F

L

L

E

A

R

R

H

Q

P

P

E

Q

V

V

S

E

W

L

V

I

S

H

Y

Y

P

P

G

G

L

L

E

A

T

S

D

F

R

P

Y

Q

H

Y

A

T

L

L

H

A

K

R

R

Q

Y

Q

C

M

P

S

L

-

G

Q

A

P

G

G

A

G

V

M

F

I

T

A

F

F

G

E

L

L

K

K

G

G

G

G

Y

I

E

G

A

A

G

G

V

R

K

R

L

F

V

M

S

N

N

A

L

L

K

Q

L

L

F

F

S

S

H

R

L

A

A
x
V

N
x
S

I
x
L

G

G

D

D

T

A

R

E

S

S

L

L

V

A

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

S

L

Y

T

T

D

P

E

E

Q

E

K

R

V

A

R

H

A

Y

G

G

A

I

G

S

P

E

E

G

V

L

V

V

R
|
R

L

L

S

S

I

V

G

G

I

L

E

E

D

D

P

I

A

D

D

D

L

L

I

L

A

A

D

D

L

V

D

Q

Q

Q

A

A

L

L

active site: R60 (= R59), Y113 (= Y112), D185 (= D184), K210 (= K209)
binding methionine: Y113 (= Y112), K210 (= K209), V338 (≠ A369), S339 (≠ N370), L340 (≠ I371), R374 (= R405)

5x30C Crystal structure of pseudomonas putida methionine gamma-lyase c116h mutant with l-homocysteine intermediates. (see paper)
41% identity, 99% coverage: 4:424/426 of query aligns to 1:389/393 of 5x30C

query
sites
5x30C

R

K

L

L

P

P

G

G

F

F

D

A

T

T

L

R

A

A

I

I

H

H

A

H

G

G

A

Y

A

D

P

P

D

Q

A

D

A

H

T

G

G

G

A

A

R

L

A

V

T

P

P

P

I

V

Y

Y

Q

Q

T

T

T

A

S

T

F

F

V

T

F

F

D

P

D

T

V

V

D

E

H

Y

A

G

A

A

S

A

L

C

F

F

G

A

L

G

Q

E

A

Q

F

A

G

G

N

H

I

F

Y
|
Y

S

S

R
|
R

I

I

G

S

N

N

P

P

T

T

N

L

A

N

V

L

L

L

E

E

E

A

R

R

V

M

A

A

A

S

L

L

E

E

G

G

G

G

T

E

A

A

A

G

L

L

A

A

V

L

A

A

S

S

G

G

H

M

A

G

A

A

Q

I

F

T

L

S

V

T

M

L

H

W

A

T

L

L

L

L

Q

R

P

P

G

G

D

D

E

E

F

V

I

L

A

L

A

G

N

N

K

T

L

L

Y
|
Y

G

G

G

H

S

T

I

F

N

A

Q

F

F

L

N

H

H

H

S

G

Y

I

K

G

N

E

F

F

G

G

W

V

S

K

V

L

A

R

W

H

A

V

D

D

T

M

D

A

D

D

P

L

A

Q

S

A

F

L

E

E

A

A

A

A

I

M

T

T

P

P

R

A

T

T

K

R

A

V

I

I

F

Y

V

F

E

E

S

S

I

P

A

A

N

N

P

P

G

N

G

M

V

H

I

M

T

A

D

D

L

I

A

A

A

G

I

V

A

A

A

K

V

I

A

A

K

R

R

K

H

H

N

G

I

A

P

T

F

V

I

V

V

V

D
|
D

N

N

T

T

M

Y

A

C

T

T

P

P

Y

Y

L

L

I

Q

R

R

P

P

F

L

E

E

H

L

G

G

A

A

D

D

I

L

V

V

V

V

H

H

S

S

A

A

T

T

K
|
K

F

Y

L

L

G

S

G

G

H

H

G

G

N

D

S

I

I

T

G

A

G

G

L

I

I

V

V

V

D

-

G

-

G

-

T

-

F

-

A

-

W

-

A

-

G

-

D

-

D

-

R

-

Y

-

P

-

M

-

L

-

S

-

K

-

P

-

R

-

P

-

E

-

Y

-

G

-

G

-

M

-

V

-

L

-

S

-

E

-

T

-

F

-

G

G

N

S

F

Q

G

A

F

L

A

V

I

D

A

R

V

I

R

R

V

L

L

Q

G

G

L

L

R

K

D

D

L

M

-

T

G

G

P

A

A

V

L

L

S

S

P

P

F

H

N

D

A

A

F

A

L

L

I

L

L

M

N

R

G

G

I

I

E

K

T

T

L

L

P

N

L

L

R

R

M

M

Q

D

R

R

H

H

S

C

D

A

N

N

A

A

R

Q

V

V

V

L

A

A

Q

E

H

F

L

L

E

A

R

R

H

Q

P

P

E

Q

V

V

S

E

W

L

V

I

S

H

Y

Y

P

P

G

G

L

L

E

A

T

S

D

F

R

P

Y

Q

H

Y

A

T

L

L

H

A

K

R

R

Q

Y

Q

C

M

P

S

L

-

G

Q

A

P

G

G

A

G

V

M

F

I

T

A

F

F

G

E

L

L

K

K

G

G

G

G

Y

I

E

G

A

A

G

G

V

R

K

R

L

F

V

M

S

N

N

A

L

L

K

Q

L

L

F

F

S

S

H

R

L

A

A

V

N

S

I

L

G

G

D

D

T

A

R

E

S

S

L

L

V

A

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

S

L

Y

T

T

D

P

E

E

Q

E

K

R

V

A

R

H

A

Y

G

G

A

I

G

S

P

E

E

G

V

L

V

V

R

R

L

L

S

S

I

V

G

G

I

L

E

E

D

D

P

I

A

D

D

D

L

L

I

L

A

A

D

D

L

V

D

Q

Q

Q

A

A

L

L

active site: R56 (= R59), Y109 (= Y112), D181 (= D184), K206 (= K209)
binding (2~{S})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]-4-sulfanyl-butanoic acid: Y54 (= Y57), R56 (= R59)

Query Sequence

>WP_015931290.1 NCBI__GCF_000022085.1:WP_015931290.1
MTDRLPGFDTLAIHAGAAPDAATGARATPIYQTTSFVFDDVDHAASLFGLQAFGNIYSRI
GNPTNAVLEERVAALEGGTAALAVASGHAAQFLVMHALLQPGDEFIAANKLYGGSINQFN
HSYKNFGWSVAWADTDDPASFEAAITPRTKAIFVESIANPGGVITDLAAIAAVAKRHNIP
FIVDNTMATPYLIRPFEHGADIVVHSATKFLGGHGNSIGGLIVDGGTFAWAGDDRYPMLS
KPRPEYGGMVLSETFGNFGFAIAVRVLGLRDLGPALSPFNAFLILNGIETLPLRMQRHSD
NARVVAQHLERHPEVSWVSYPGLETDRYHALHKRYCPLGAGAVFTFGLKGGYEAGVKLVS
NLKLFSHLANIGDTRSLVIHPASTTHRQLTDEQKVRAGAGPEVVRLSIGIEDPADLIADL
DQALAG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory