SitesBLAST
Comparing WP_015932725.1 NCBI__GCF_000022085.1:WP_015932725.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P12995 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Escherichia coli (strain K12) (see 4 papers)
52% identity, 97% coverage: 9:409/415 of query aligns to 13:418/429 of P12995
- Y17 (= Y13) mutation to F: Severely reduces the aminotransferase activity.
- W52 (= W48) binding substrate
- GS 112:113 (= GS 108:109) binding pyridoxal 5'-phosphate
- Y144 (= Y141) mutation to F: Severely reduces the aminotransferase activity.
- D147 (= D144) mutation to N: Loss of aminotransferase activity.
- D245 (= D235) binding pyridoxal 5'-phosphate
- R253 (= R243) mutation to A: Has only a small effect on the rate of reaction with DAPA.; mutation to K: Increases aminotransferase activity toward SAM.; mutation to M: Loss of aminotransferase activity.; mutation to Q: Increases aminotransferase activity toward SAM.
- K274 (= K264) binding substrate; modified: N6-(pyridoxal phosphate)lysine
- G307 (= G298) binding substrate
- PT 308:309 (= PT 299:300) binding pyridoxal 5'-phosphate
- R391 (= R382) binding substrate; mutation to A: Reduces aminotransferase activity.
6ed7A Crystal structure of 7,8-diaminopelargonic acid synthase bound to inhibitor mac13772
52% identity, 97% coverage: 9:409/415 of query aligns to 13:418/429 of 6ed7A
- active site: Y17 (= Y13), Y144 (= Y141), D245 (= D235), K274 (= K264)
- binding 2-[(2-nitrophenyl)sulfanyl]acetohydrazide: Y17 (= Y13), W52 (= W48), W52 (= W48), Y144 (= Y141), D147 (= D144), A217 (= A207), K274 (= K264), R391 (= R382), F393 (= F384), F393 (= F384)
- binding pyridoxal-5'-phosphate: G112 (= G108), S113 (= S109), Y144 (= Y141), H145 (= H142), D245 (= D235), I247 (≠ V237), K274 (= K264)
1dtyA Crystal structure of adenosylmethionine-8-amino-7-oxonanoate aminotransferase with pyridoxal phosphate cofactor.
52% identity, 97% coverage: 9:409/415 of query aligns to 13:418/429 of 1dtyA
- active site: Y17 (= Y13), Y144 (= Y141), E211 (= E201), D245 (= D235), A248 (≠ F238), K274 (= K264), Y398 (= Y389)
- binding pyridoxal-5'-phosphate: G112 (= G108), S113 (= S109), Y144 (= Y141), H145 (= H142), D245 (= D235), I247 (≠ V237), K274 (= K264)
1mlzA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the trans-isomer of amiclenomycin. (see paper)
52% identity, 96% coverage: 11:409/415 of query aligns to 15:417/427 of 1mlzA
- active site: Y17 (= Y13), Y144 (= Y141), E210 (= E201), D244 (= D235), A247 (≠ F238), K273 (= K264), Y397 (= Y389)
- binding pyridoxal-5'-phosphate: G112 (= G108), S113 (= S109), Y144 (= Y141), H145 (= H142), D244 (= D235), I246 (≠ V237), K273 (= K264), P307 (= P299), T308 (= T300)
- binding trans-amiclenomycin: W52 (= W48), W53 (= W49), Y144 (= Y141), K273 (= K264), R390 (= R382), F392 (= F384)
1mlyA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the cis isomer of amiclenomycin (see paper)
52% identity, 96% coverage: 11:409/415 of query aligns to 15:417/427 of 1mlyA
- active site: Y17 (= Y13), Y144 (= Y141), E210 (= E201), D244 (= D235), A247 (≠ F238), K273 (= K264), Y397 (= Y389)
- binding cis-amiclenomycin: W52 (= W48), W53 (= W49), K273 (= K264), R390 (= R382), F392 (= F384)
- binding pyridoxal-5'-phosphate: G112 (= G108), S113 (= S109), Y144 (= Y141), H145 (= H142), D244 (= D235), I246 (≠ V237), K273 (= K264), P307 (= P299), T308 (= T300)
1qj3A Crystal structure of 7,8-diaminopelargonic acid synthase in complex with 7-keto-8-aminopelargonic acid (see paper)
51% identity, 96% coverage: 11:409/415 of query aligns to 15:406/416 of 1qj3A
- active site: Y17 (= Y13), Y144 (= Y141), E201 (= E201), D235 (= D235), A238 (≠ F238), K264 (= K264), Y386 (= Y389)
- binding 7-keto-8-aminopelargonic acid: Y17 (= Y13), W52 (= W48), Y144 (= Y141), K264 (= K264), R379 (= R382), F381 (= F384)
- binding pyridoxal-5'-phosphate: G112 (= G108), S113 (= S109), Y144 (= Y141), H145 (= H142), G146 (= G143), D235 (= D235), I237 (≠ V237), A238 (≠ F238), K264 (= K264)
4cxqA Mycobaterium tuberculosis transaminase bioa complexed with substrate kapa (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:421/427 of 4cxqA
- active site: Y18 (= Y13), Y149 (= Y141), E212 (= E201), D246 (= D235), A249 (≠ F238), K275 (= K264), Y399 (= Y389)
- binding 7-keto-8-aminopelargonic acid: W56 (= W48), Y149 (= Y141), G308 (= G298), T310 (= T300), R392 (= R382)
- binding pyridoxal-5'-phosphate: G116 (= G108), S117 (= S109), Y149 (= Y141), H150 (= H142), G151 (= G143), E212 (= E201), D246 (= D235), I248 (≠ V237), K275 (= K264), P309 (= P299), T310 (= T300)
4w1vA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a thiazole inhibitor (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:419/425 of 4w1vA
- active site: Y18 (= Y13), Y147 (= Y141), E210 (= E201), D244 (= D235), A247 (≠ F238), K273 (= K264), Y397 (= Y389)
- binding dimethyl (2R)-5-(3-fluorophenyl)-1H-pyrrolo[1,2-c][1,3]thiazole-6,7-dicarboxylate 2-oxide: P17 (= P12), Y18 (= Y13), W54 (= W48), M81 (= M75), G83 (= G77), Y147 (= Y141), G306 (= G298), P307 (= P299), T308 (= T300), F392 (= F384)
- binding pyridoxal-5'-phosphate: G114 (= G108), S115 (= S109), Y147 (= Y141), H148 (= H142), E210 (= E201), D244 (= D235), I246 (≠ V237), K273 (= K264)
4cxrA Mycobaterium tuberculosis transaminase bioa complexed with 1-(1,3- benzothiazol-2-yl)methanamine (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:419/425 of 4cxrA
- active site: Y18 (= Y13), Y147 (= Y141), E210 (= E201), D244 (= D235), A247 (≠ F238), K273 (= K264), Y397 (= Y389)
- binding 1-(1,3-benzothiazol-2-yl)methanamine: Y18 (= Y13), W54 (= W48), W55 (= W49), A216 (= A207)
- binding pyridoxal-5'-phosphate: G114 (= G108), S115 (= S109), Y147 (= Y141), H148 (= H142), E210 (= E201), D244 (= D235), I246 (≠ V237), K273 (= K264), P307 (= P299), T308 (= T300)
5te2A Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a mechanism-based inhibitor (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:422/428 of 5te2A
- active site: Y18 (= Y13), Y150 (= Y141), E213 (= E201), D247 (= D235), A250 (≠ F238), K276 (= K264), Y400 (= Y389)
- binding [5-hydroxy-4-({[6-(3-hydroxypropyl)-4-oxo-1,4-dihydropyridin-3-yl]amino}methyl)-6-methylpyridin-3-yl]methyl dihydrogen phosphate: Y18 (= Y13), W57 (= W48), G117 (= G108), S118 (= S109), Y150 (= Y141), H151 (= H142), G152 (= G143), D247 (= D235), I249 (≠ V237), K276 (= K264), G309 (= G298), P310 (= P299), T311 (= T300)
4xjoA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:422/428 of 4xjoA
- active site: Y18 (= Y13), Y150 (= Y141), E213 (= E201), D247 (= D235), A250 (≠ F238), K276 (= K264), Y400 (= Y389)
- binding 5-[4-(3-chlorobenzoyl)piperazin-1-yl]-1H-inden-1-one: P17 (= P12), Y18 (= Y13), W57 (= W48), M84 (= M75), G86 (= G77), Y150 (= Y141), G165 (≠ E156), G166 (= G157), A219 (= A207), P310 (= P299), T311 (= T300)
- binding pyridoxal-5'-phosphate: G117 (= G108), S118 (= S109), Y150 (= Y141), H151 (= H142), G152 (= G143), E213 (= E201), D247 (= D235), I249 (≠ V237), K276 (= K264), P310 (= P299), T311 (= T300)
4xewA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a hts lead compound (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:422/428 of 4xewA
- active site: Y18 (= Y13), Y150 (= Y141), E213 (= E201), D247 (= D235), A250 (≠ F238), K276 (= K264), Y400 (= Y389)
- binding 6-(2-fluorophenyl)[1,3]dioxolo[4,5-g]quinolin-8(5H)-one: P17 (= P12), Y18 (= Y13), W57 (= W48), Y150 (= Y141), P310 (= P299), T311 (= T300), R393 (= R382), F395 (= F384)
- binding pyridoxal-5'-phosphate: G117 (= G108), S118 (= S109), Y150 (= Y141), H151 (= H142), G152 (= G143), E213 (= E201), D247 (= D235), I249 (≠ V237), K276 (= K264), P310 (= P299), T311 (= T300)
4wyfA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a dsf fragment hit (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:422/428 of 4wyfA
- active site: Y18 (= Y13), Y150 (= Y141), E213 (= E201), D247 (= D235), A250 (≠ F238), K276 (= K264), Y400 (= Y389)
- binding N-(1-oxo-1H-inden-5-yl)acetamide: M84 (= M75), G86 (= G77), G309 (= G298), P310 (= P299), T311 (= T300)
- binding pyridoxal-5'-phosphate: G117 (= G108), S118 (= S109), Y150 (= Y141), H151 (= H142), G152 (= G143), E213 (= E201), D247 (= D235), I249 (≠ V237), K276 (= K264), P310 (= P299), T311 (= T300)
4wycA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a thiazole benzamide inhibitor (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:422/428 of 4wycA
- active site: Y18 (= Y13), Y150 (= Y141), E213 (= E201), D247 (= D235), A250 (≠ F238), K276 (= K264), Y400 (= Y389)
- binding 4-(1H-imidazol-1-yl)benzamide: P17 (= P12), Y18 (= Y13), W57 (= W48), M84 (= M75), G86 (= G77), Y150 (= Y141), G309 (= G298)
- binding pyridoxal-5'-phosphate: G117 (= G108), S118 (= S109), Y150 (= Y141), H151 (= H142), G152 (= G143), E213 (= E201), D247 (= D235), I249 (≠ V237), K276 (= K264), P310 (= P299), T311 (= T300)
4mqrA Mycobaterium tuberculosis transaminase bioa complexed with e)-5- hydroxy-4-(((z)-isonicotinoyldiazenyl)methylene)-6-methyl-1,4- dihydropyridin-3-yl)methyl phosphate (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:422/428 of 4mqrA
- active site: Y18 (= Y13), Y150 (= Y141), E213 (= E201), D247 (= D235), A250 (≠ F238), K276 (= K264), Y400 (= Y389)
- binding [(4Z)-5-hydroxy-6-methyl-4-{[(E)-(pyridin-4-ylcarbonyl)diazenyl]methylidene}-1,4-dihydropyridin-3-yl]methyl dihydrogen phosphate: Y18 (= Y13), W57 (= W48), G117 (= G108), S118 (= S109), Y150 (= Y141), H151 (= H142), D247 (= D235), I249 (≠ V237), K276 (= K264), G309 (= G298), P310 (= P299), T311 (= T300)
4mqqA Mycobaterium tuberculosis transaminase bioa complexed with benzo[d]thiazole-2-carbohydrazide (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:422/428 of 4mqqA
- active site: Y18 (= Y13), Y150 (= Y141), E213 (= E201), D247 (= D235), A250 (≠ F238), K276 (= K264), Y400 (= Y389)
- binding (4-{[(E)-(1,3-benzothiazol-2-ylcarbonyl)diazenyl]methyl}-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate: Y18 (= Y13), W57 (= W48), W58 (= W49), G117 (= G108), S118 (= S109), Y150 (= Y141), H151 (= H142), D247 (= D235), I249 (≠ V237), K276 (= K264), P310 (= P299), T311 (= T300)
4mqpA Mycobaterium tuberculosis transaminase bioa complexed with 2- hydrazinylbenzo[d]thiazole (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:422/428 of 4mqpA
- active site: Y18 (= Y13), Y150 (= Y141), E213 (= E201), D247 (= D235), A250 (≠ F238), K276 (= K264), Y400 (= Y389)
- binding (4-{[(E)-1,3-benzothiazol-2-yldiazenyl]methyl}-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate: W57 (= W48), W58 (= W49), G117 (= G108), S118 (= S109), Y150 (= Y141), H151 (= H142), E213 (= E201), A219 (= A207), D247 (= D235), I249 (≠ V237), K276 (= K264), Y400 (= Y389)
5kgtA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead: 1-[4-[4-(3-chlorophenyl)carbonylpiperidin-1- yl]phenyl]ethanone (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:422/429 of 5kgtA
- active site: Y18 (= Y13), Y150 (= Y141), E213 (= E201), D247 (= D235), A250 (≠ F238), K276 (= K264), Y400 (= Y389)
- binding 1-[4-[4-(3-chlorophenyl)carbonylpiperidin-1-yl]phenyl]ethanone: M84 (= M75), G86 (= G77), G309 (= G298), T311 (= T300)
- binding pyridoxal-5'-phosphate: S116 (= S107), G117 (= G108), S118 (= S109), Y150 (= Y141), H151 (= H142), G152 (= G143), E213 (= E201), D247 (= D235), I249 (≠ V237), K276 (= K264)
5kgsA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead: 5-[4-(1,3-benzodioxol-5-ylcarbonyl)piperazin-1-yl]-2, 3-dihydroinden-1-one (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:422/429 of 5kgsA
- active site: Y18 (= Y13), Y150 (= Y141), E213 (= E201), D247 (= D235), A250 (≠ F238), K276 (= K264), Y400 (= Y389)
- binding 5-[4-(1,3-benzodioxol-5-ylcarbonyl)piperazin-1-yl]-2,3-dihydroinden-1-one: P17 (= P12), Y18 (= Y13), W57 (= W48), M84 (= M75), G86 (= G77), Y150 (= Y141), D162 (= D153), G165 (≠ E156), G166 (= G157), P310 (= P299), T311 (= T300), F395 (= F384)
- binding pyridoxal-5'-phosphate: G117 (= G108), S118 (= S109), Y150 (= Y141), H151 (= H142), G152 (= G143), E213 (= E201), D247 (= D235), I249 (≠ V237), K276 (= K264)
4xjpA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead (see paper)
49% identity, 97% coverage: 9:411/415 of query aligns to 14:422/429 of 4xjpA
- active site: Y18 (= Y13), Y150 (= Y141), E213 (= E201), D247 (= D235), A250 (≠ F238), K276 (= K264), Y400 (= Y389)
- binding 1-{4-[4-(1,3-benzodioxol-5-ylcarbonyl)piperazin-1-yl]phenyl}ethanone: P17 (= P12), Y18 (= Y13), W57 (= W48), M84 (= M75), G86 (= G77), Y150 (= Y141), G165 (≠ E156), G166 (= G157), A219 (= A207), G220 (= G208), G309 (= G298), F395 (= F384)
- binding pyridoxal-5'-phosphate: G117 (= G108), S118 (= S109), Y150 (= Y141), H151 (= H142), G152 (= G143), E213 (= E201), D247 (= D235), I249 (≠ V237), K276 (= K264), P310 (= P299), T311 (= T300)
Query Sequence
>WP_015932725.1 NCBI__GCF_000022085.1:WP_015932725.1
MSEPRDDLLWRPYTQMKTAAPPLRAARTQGSRIVLEDGRELVDGIASWWTACHGYNHPHI
RQAVAAQLDAMPHVMFGGLTHAPAEDLARRLAALLPGDLDHVFFSDSGSVAVEVALKAAA
QVWLNRGVAGRSRFAAFRGGYHGDTMGAMSVCDPEEGMHRRFGRYLPEQLFFDLPDTRAR
ETAVDAGLARHRDTLAGVIVEPLVQGAGGMRMHPPEVLATVARLARRHGLILILDEVFTG
FGRTGTLFACEQAGVVPDLICLSKALTGGTLPLAATVATAEIFAAFWSDDPAAALMHGPT
FMANPLACAAANASLDLFAREPRLAQANRIAAQLEEGLAPLRGRPGIRDVRVLGAIGAVQ
LVPPTDLAGMKAAFLERGAWVRPFGDIVYLTPALTIPEEDLARLTGAMREILGAA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory