SitesBLAST
Comparing WP_015933040.1 NCBI__GCF_000022085.1:WP_015933040.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5itoA Structure of the periplasmic binding protein m117n-noct from a. Tumefaciens in complex with octopine (see paper)
33% identity, 89% coverage: 28:254/256 of query aligns to 5:252/255 of 5itoA
- binding octopine: E10 (= E33), Y13 (≠ N36), W51 (= W73), A68 (= A90), A69 (≠ S91), G71 (≠ A93), R76 (= R98), Q139 (≠ A139), T142 (≠ S142), S143 (≠ P143), H144 (≠ Q144), S181 (≠ D181)
5otcA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with noroctopinic acid. (see paper)
33% identity, 89% coverage: 28:254/256 of query aligns to 4:251/256 of 5otcA
- binding (2~{S})-5-azanyl-2-(2-hydroxy-2-oxoethylamino)pentanoic acid: E9 (= E33), Y12 (≠ N36), W50 (= W73), A68 (≠ S91), G70 (≠ A93), R75 (= R98), Q138 (≠ A139), T141 (≠ S142), S142 (≠ P143), H143 (≠ Q144), S180 (≠ D181)
5ovzA High resolution structure of the pbp noct in complex with nopaline (see paper)
33% identity, 89% coverage: 28:254/256 of query aligns to 5:252/259 of 5ovzA
- binding N-[(1S)-4-carbamimidamido-1-carboxybutyl]-D-glutamic acid: E10 (= E33), Y13 (≠ N36), W51 (= W73), A68 (= A90), A69 (≠ S91), G71 (≠ A93), R76 (= R98), M91 (≠ S113), Q139 (≠ A139), T142 (≠ S142), S143 (≠ P143), H144 (≠ Q144), S181 (≠ D181), V213 (= V215)
5otaA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with octopinic acid (see paper)
33% identity, 89% coverage: 28:254/256 of query aligns to 4:251/254 of 5otaA
- binding (2~{S})-5-azanyl-2-[[(2~{R})-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]pentanoic acid: E9 (= E33), Y12 (≠ N36), W50 (= W73), A68 (≠ S91), G70 (≠ A93), R75 (= R98), M90 (≠ S113), Q138 (≠ A139), T141 (≠ S142), S142 (≠ P143), H143 (≠ Q144), S180 (≠ D181)
5ot9A Structure of the periplasmic binding protein (pbp) noct from a.Tumefaciens c58 in complex with histopine. (see paper)
33% identity, 89% coverage: 28:254/256 of query aligns to 4:251/254 of 5ot9A
- binding Histopine: E9 (= E33), Y12 (≠ N36), W50 (= W73), A67 (= A90), A68 (≠ S91), R75 (= R98), M90 (≠ S113), Q138 (≠ A139), T141 (≠ S142), S142 (≠ P143), H143 (≠ Q144), S180 (≠ D181)
4powA Structure of the pbp noct in complex with pyronopaline (see paper)
33% identity, 89% coverage: 28:254/256 of query aligns to 4:251/254 of 4powA
- binding 1-[(1S)-4-carbamimidamido-1-carboxybutyl]-5-oxo-D-proline: E9 (= E33), Y12 (≠ N36), W50 (= W73), A67 (= A90), A68 (≠ S91), G70 (≠ A93), R75 (= R98), M90 (≠ S113), Q138 (≠ A139), T141 (≠ S142), S142 (≠ P143), H143 (≠ Q144), S180 (≠ D181)
P02911 Lysine/arginine/ornithine-binding periplasmic protein; LAO-binding protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
34% identity, 99% coverage: 1:254/256 of query aligns to 1:256/260 of P02911
- D33 (≠ E33) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- Y36 (≠ N36) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- D52 (≠ E51) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- C60 (= C59) modified: Disulfide link with 67
- C67 (= C66) modified: Disulfide link with 60
- F74 (≠ W73) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- S91 (≠ A90) binding L-arginine; binding L-ornithine; mutation to A: Has no effect on the arginine and histidine binding affinity.
- S92 (= S91) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- S94 (≠ A93) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- R99 (= R98) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- L139 (≠ A139) mutation to K: Changes ligand specificity. Can bind glutamine. Still able to bind basic amino acids, however, with 1000-fold less affinity for arginine.; mutation to Q: Does not affect ligand preference.
- T143 (≠ P143) binding L-arginine; binding L-lysine; binding L-ornithine
- D183 (= D181) binding L-arginine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
5orgA Structure of the periplasmic binding protein (pbp) occj from a. Tumefaciens b6 in complex with octopine. (see paper)
35% identity, 89% coverage: 28:254/256 of query aligns to 6:253/257 of 5orgA
- binding octopine: E11 (= E33), Y14 (≠ N36), W52 (= W73), A69 (= A90), A70 (≠ S91), M71 (≠ L92), S72 (≠ A93), R77 (= R98), N92 (≠ S113), Q140 (≠ A139), T143 (≠ S142), T144 (≠ P143), N183 (≠ D181)
P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
35% identity, 90% coverage: 24:254/256 of query aligns to 24:256/260 of P02910
- E40 (≠ N40) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
- K42 (≠ V42) mutation to E: Increases ATPase-inducing activity and histidine transport; when associated with K-47.
- E47 (≠ K46) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
- D171 (= D169) mutation D->A,N: Strong decrease in ATPase-inducing activity and histidine transport.
- R176 (= R174) mutation R->D,S: Strong decrease in ATPase-inducing activity and histidine transport.
- D178 (= D176) mutation to A: Slight decrease in ATPase-inducing activity and histidine transport.
Sites not aligning to the query:
1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
35% identity, 90% coverage: 24:254/256 of query aligns to 2:234/238 of 1hslA
1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand (see paper)
35% identity, 90% coverage: 24:254/256 of query aligns to 2:234/238 of 1lstA
1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
35% identity, 90% coverage: 24:254/256 of query aligns to 2:234/238 of 1lahE
1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
35% identity, 90% coverage: 24:254/256 of query aligns to 2:234/238 of 1lagE
- binding histidine: Y14 (≠ N36), F52 (≠ W73), S69 (≠ A90), S70 (= S91), L71 (= L92), S72 (≠ A93), R77 (= R98), L117 (≠ A139), S120 (= S142), Q122 (= Q144), D161 (= D181)
1lafE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
35% identity, 90% coverage: 24:254/256 of query aligns to 2:234/238 of 1lafE
- binding arginine: D11 (≠ E33), Y14 (≠ N36), F52 (≠ W73), S69 (≠ A90), S70 (= S91), S72 (≠ A93), R77 (= R98), L117 (≠ A139), S120 (= S142), T121 (≠ P143), Q122 (= Q144), D161 (= D181)
P0AEU0 Histidine-binding periplasmic protein; HBP from Escherichia coli (strain K12) (see 3 papers)
34% identity, 90% coverage: 24:254/256 of query aligns to 24:256/260 of P0AEU0
- C60 (= C59) modified: Disulfide link with 67
- C67 (= C66) modified: Disulfide link with 60
- S91 (≠ A90) binding L-histidine
- S92 (= S91) binding L-histidine
- S94 (≠ A93) binding L-histidine
- R99 (= R98) binding L-histidine
- T143 (≠ P143) binding L-histidine
- D183 (= D181) binding L-histidine
Sites not aligning to the query:
3i6vA Crystal structure of a periplasmic his/glu/gln/arg/opine family- binding protein from silicibacter pomeroyi in complex with lysine
38% identity, 89% coverage: 28:254/256 of query aligns to 2:214/218 of 3i6vA
- binding lysine: E7 (= E33), Y10 (≠ N36), W48 (= W73), G66 (≠ S91), M67 (≠ L92), S68 (≠ A93), R73 (= R98), Q107 (≠ A139), T110 (≠ S142), I111 (≠ P143), Q112 (= Q144)
4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
31% identity, 88% coverage: 28:251/256 of query aligns to 6:223/225 of 4zv2A
5owfA Structure of a lao-binding protein mutant with glutamine (see paper)
34% identity, 89% coverage: 28:254/256 of query aligns to 3:231/235 of 5owfA
4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
31% identity, 88% coverage: 28:251/256 of query aligns to 6:225/226 of 4zv1A