SitesBLAST

399 C→A: Strongly decreased nickel inhibition; when associated with A-402, A-410 and A-413.; C→S: No effect on inostol transport or nickel inhibition. No effect on inostol transport or nickel inhibition; when associated with S-410.
402 C→A: Strongly decreased nickel inhibition; when associated with A-399, A-410 and A-413.
410 C→A: Strongly decreased nickel inhibition; when associated with A-399, A-402 and A-413.; C→S: No effect on inostol transport or nickel inhibition; when associated with S-399.
413 C→A: Strongly decreased nickel inhibition; when associated with A-399, A-402 and A-410.

O15244 Solute carrier family 22 member 2; Organic cation transporter 2; hOCT2 from Homo sapiens (Human) (see 8 papers)
24% identity, 65% coverage: 75:378/468 of query aligns to 158:467/555 of O15244

query
sites
O15244

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M165 (≠ W85) to I: lower Vmax for MPP(+) transport; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177507
Y169 (≠ W89) mutation to F: No change in TEA uptake.
T201 (≠ Q121) to M: in dbSNP:rs145450955
Y241 (≠ Q169) mutation to F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-362 and F-377.
Y257 (≠ T185) mutation to F: No change in TEA uptake.
S270 (≠ G208) to A: decreased Ki value for TBA inhibition of MPP(+); no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs316019
Y279 (= Y217) mutation to F: No change in TEA uptake.
Y280 (≠ L218) mutation to F: No change in TEA uptake.
P284 (vs. gap) mutation to A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.
PESPR 284:288 (≠ ----R 219) Proline-rich sequence
S286 (vs. gap) mutation to A: No change in TEA and metformin uptake. No change in tyrosine phosphorylation.
P287 (vs. gap) mutation to A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.
Y362 (≠ M299) mutation to F: Decreased TEA uptake and YES1-mediated tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-241. Strong decrease in TEA uptake; when associated with F-377. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-377.
Y377 (≠ G311) mutation to F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-362.
R400 (= R335) to C: lower Vmax and reduced Ki value for TBA inhibition of MPP(+); lower transport efficiency (Vmax/Km) and clearance of cyclo(his-pro); no change in transport efficiency (Vmax/Km) and clearance of salsolinol; dbSNP:rs8177516
K432 (vs. gap) to Q: lower Km value for MPP(+) and reduced Ki value for TBA inhibition of MPP; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177517
Y458 (= Y369) mutation to F: No change in TEA uptake.

Sites not aligning to the query:

54 P → S: in dbSNP:rs8177504
73 Y→F: No change in TEA uptake.
92 Y→F: No change in TEA uptake.
128 Y→F: No change in TEA uptake.
544 Y→F: No change in TEA uptake.

P11168 Solute carrier family 2, facilitated glucose transporter member 2; Glucose transporter type 2, liver; GLUT-2 from Homo sapiens (Human) (see 6 papers)
33% identity, 22% coverage: 88:191/468 of query aligns to 116:212/524 of P11168

query
sites
P11168

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V197 (= V174) to I: in NIDDM; abolishes transport activity of the transporter expressed in Xenopus oocytes; dbSNP:rs121909741

Sites not aligning to the query:

68 P → L: in dbSNP:rs7637863
110 T → I: in dbSNP:rs5400
322 I→V: Reduced fructose transport.

8fvzA Pipt y150a
24% identity, 45% coverage: 24:235/468 of query aligns to 2:214/433 of 8fvzA

query
sites
8fvzA

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binding phosphate ion: F145 (≠ G166)

Sites not aligning to the query:

binding phosphate ion: 235, 239, 243, 346

Q9Y7Q9 Probable metabolite transporter C2H8.02 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
27% identity, 46% coverage: 22:238/468 of query aligns to 36:259/583 of Q9Y7Q9

query
sites
Q9Y7Q9

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Sites not aligning to the query:

267 modified: Phosphoserine
269 modified: Phosphoserine
289 modified: Phosphoserine
290 modified: Phosphoserine
292 modified: Phosphoserine
330 modified: Phosphoserine

Query Sequence

>WP_015933119.1 NCBI__GCF_000022085.1:WP_015933119.1
MSEPLAPSYPGGLSLQVRRRGRERVRAMLGACAGNLVEWYDFFVYAYASIYFAGAFFPKG
DQTSQLLATAGVFAVGFFMRPIGGWLFGWIADTRGRKIAMVISVMMMSAGSLLIAILPTY
QAIGIAAPILLVMARMFQGLSVGAEYGTGATYISEIATKGRRGFYGSFQYFTIVAGQFLA
LSTVTALQHLLTLDELRAWGWRIPFIIGALSAVVVFYLRRTMTETGSEKAMHRKEAGSLK
GLLAHKRAVLLVLAFTAGGSLYFYTFTTYMQKFLVVSAGMKPETVSIVMTVSLVSFMLMQ
PVFGLLSDRLGVKIHMLLFTGLAALFVVPILTLIRSVTDPVAATGLVIAGLAIAAFYTPV
SGLVKADLYPASVRALGVGFPYAIANAMFGGTAEYVALFLRSNGAESWFYVYVAVLAAFA
FVASVAMPELTRHGYLDGSGTIKGNTGFRRDPARRAKALPAEVDTGSA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory