SitesBLAST

E189 (= E264) binding Mg(2+)
V190 (= V265) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E271) binding Mg(2+)
G241 (= G316) binding L-glutamate
H245 (= H320) binding Mg(2+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
59 G→R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
62 Important for inhibition by glutamine; R→A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
132 binding Mg(2+)
134 binding Mg(2+)
302 G→E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
304 E→A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
306 P→H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
333 binding Mg(2+)
424 E→K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
36% identity, 13% coverage: 246:335/695 of query aligns to 170:259/443 of 4lnkA

query
sites
4lnkA

D

D

V

I

N

V

V

L

E

E

A

L

W

E

K

E

L

M

G

G

I

F

A

E

A

I

K

E

T

A

Q

S

H

H

K

H

E
|
E

V
|
V

A

A

P

P

G

G

Q

Q

Y

H

E
|
E

I

I

A

D

P
x
F

I

K

F
x
Y

S

A

V

G

T

A

N

V

V

R

A

S

T

C

D

D

H

D

N

I

Q

Q

L

T

I

F

M

K

D

L

I

V

L

V

K

K

K

T

V

I

A

A

N

R

R

K

H

H

G

G

L

L

V

H

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

L

A

F

G

G

V

V

N
|
N

G
|
G

S

S

G
|
G

K

M

H
|
H

N

C

N
|
N

W

L

A
x
S

L

L

G

F

T

K

N

N

S

G

G

V

K

N

N

A

L

F

D

D

active site: E188 (= E264), E195 (= E271), H244 (= H320)
binding adenosine-5'-diphosphate: F198 (≠ P274), Y200 (≠ F276), N246 (= N322), S248 (≠ A324)
binding glutamic acid: E188 (= E264), V189 (= V265), N239 (= N315), G240 (= G316), G242 (= G318)
binding magnesium ion: E188 (= E264), E195 (= E271), H244 (= H320)

Sites not aligning to the query:

active site: 52, 131, 133, 315, 332, 334
binding adenosine-5'-diphosphate: 43, 50, 324, 328, 330
binding glutamic acid: 133, 303
binding magnesium ion: 131, 332

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
36% identity, 13% coverage: 246:335/695 of query aligns to 170:259/443 of 4lniA

query
sites
4lniA

D

D

V

I

N

V

V

L

E

E

A

L

W

E

K

E

L

M

G

G

I

F

A

E

A

I

K
x
E

T

A

Q

S

H

H

K

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

Y

H

E
|
E

I

I

A
x
D

P

F

I

K

F
x
Y

S

A

V

G

T

A

N

V

V

R

A

S

T

C

D

D

H

D

N

I

Q

Q

L

T

I

F

M

K

D

L

I

V

L

V

K

K

K

T

V

I

A

A

N

R

R

K

H

H

G

G

L

L

V

H

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

L

A

F

G

G

V

V

N

N

G

G

S

S

G

G

K

M

H
|
H

N

C

N
|
N

W

L

A
x
S

L

L

G

F

T

K

N

N

S

G

G

V

K

N

N

A

L

F

D

D

active site: E188 (= E264), E195 (= E271), H244 (= H320)
binding adenosine-5'-diphosphate: E183 (≠ K259), D197 (≠ A273), Y200 (≠ F276), N246 (= N322), S248 (≠ A324)
binding magnesium ion: E188 (= E264), E195 (= E271), E195 (= E271), H244 (= H320)
binding l-methionine-s-sulfoximine phosphate: E188 (= E264), H244 (= H320)

Sites not aligning to the query:

active site: 52, 131, 133, 315, 332, 334
binding adenosine-5'-diphosphate: 131, 320, 330
binding magnesium ion: 131, 131, 133, 332
binding l-methionine-s-sulfoximine phosphate: 133, 297, 303, 315, 334

4s0rD Structure of gs-tnra complex (see paper)
36% identity, 13% coverage: 246:335/695 of query aligns to 174:263/447 of 4s0rD

query
sites
4s0rD

D

D

V

I

N

V

V

L

E

E

A

L

W

E

K

E

L

M

G

G

I

F

A

E

A

I

K

E

T

A

Q

S

H

H

K

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

Y

H

E
|
E

I

I

A

D

P

F

I

K

F

Y

S

A

V

G

T

A

N

V

V

R

A

S

T

C

D

D

H

D

N

I

Q

Q

L

T

I

F

M

K

D

L

I

V

L

V

K

K

K

T

V

I

A

A

N

R

R

K

H

H

G

G

L

L

V

H

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

L

A

F

G
|
G

V
|
V

N
|
N

G

G

S

S

G

G

K

M

H
|
H

N

C

N

N

W

L

A

S

L

L

G

F

T

K

N

N

S

G

G

V

K

N

N

A

L

F

D

D

active site: E192 (= E264), E199 (= E271), H248 (= H320)
binding glutamine: E192 (= E264)
binding magnesium ion: E199 (= E271), H248 (= H320), H248 (= H320)
binding : G241 (= G313), V242 (= V314), N243 (= N315)

Sites not aligning to the query:

active site: 56, 135, 137, 319, 336, 338
binding glutamine: 137, 301, 307
binding magnesium ion: 66, 135, 135, 336, 419
binding : 63, 64, 65, 66, 161, 305, 306, 376, 426, 430

7tf6A Glutamine synthetase (see paper)
33% identity, 16% coverage: 246:355/695 of query aligns to 165:274/438 of 7tf6A

query
sites
7tf6A

D

D

V

I

N

V

V

L

E

E

A

L

W

E

K

D

L

M

G

G

I

F

A

D

A

I

K

E

T

A

Q

S

H

H

K

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

Y

H

E
|
E

I

I

A

D

P

F

I

K

F

Y

S

A

V

D

T

A

N

V

V

T

A

A

T

C

D

D

H

N

N

I

Q

Q

L

T

I

F

M

K

D

L

I

V

L

V

K

K

K

T

V

I

A

A

N

R

R

K

H

H

G

N

L

L

V

H

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

L

A

F

G
|
G

V

V

N
|
N

G
|
G

S

S

G

G

K

M

H
|
H

N

F

N

N

W

V

A

S

L

L

G

F

T

K

N

G

S

K

G

E

K

N

N

A

L

F

D

D

P

P

G

N

R

T

T

E

P

M

H

G

E

L

N

T

A

E

Q

T

F

A

L

Y

T

Q

F

F

L

T

C

A

A

G

V

V

L

L

K

K

binding glutamine: E183 (= E264), G235 (= G316), H239 (= H320)
binding magnesium ion: E183 (= E264), E190 (= E271), H239 (= H320)
binding : G232 (= G313), N234 (= N315)

Sites not aligning to the query:

binding glutamine: 128, 292, 298
binding magnesium ion: 126, 128, 327
binding : 58, 60, 296, 297, 310, 367, 421, 433, 437

7tdvC Glutamine synthetase (see paper)
32% identity, 16% coverage: 245:355/695 of query aligns to 169:279/443 of 7tdvC

query
sites
7tdvC

H

R

D

D

V

I

N

V

V

L

E

E

A

L

W

E

K

D

L

M

G

G

I

F

A

D

A

I

K
x
E

T

A

Q

S

H

H

K

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

Y

H

E
|
E

I

I

A
x
D

P
x
F

I
x
K

F
x
Y

S

A

V

D

T

A

N

V

V

T

A

A

T

C

D

D

H

N

N

I

Q

Q

L

T

I

F

M

K

D

L

I

V

L

V

K

K

K

T

V

I

A

A

N

R

R

K

H

H

G

N

L

L

V

H

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

L

A

F

G

G

V

V

N

N

G
|
G

S

S

G

G

K

M

H
|
H

N

F

N
|
N

W
x
V

A
x
S

L

L

G

F

T

K

N

G

S

K

G

E

K

N

N

A

L

F

D

D

P

P

G

N

R

T

T

E

P

M

H

G

E

L

N

T

A

E

Q

T

F

A

L

Y

T

Q

F

F

L

T

C

A

A

G

V

V

L

L

K

K

binding adenosine-5'-diphosphate: E183 (≠ K259), D197 (≠ A273), F198 (≠ P274), K199 (≠ I275), Y200 (≠ F276), N246 (= N322), V247 (≠ W323), S248 (≠ A324)
binding magnesium ion: E188 (= E264), E195 (= E271), E195 (= E271), H244 (= H320)
binding l-methionine-s-sulfoximine phosphate: E188 (= E264), E195 (= E271), G240 (= G316), H244 (= H320)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 129, 131, 320, 328, 330
binding magnesium ion: 131, 131, 133, 332
binding l-methionine-s-sulfoximine phosphate: 131, 133, 297, 303, 315

7tfaB Glutamine synthetase (see paper)
30% identity, 21% coverage: 181:325/695 of query aligns to 117:247/441 of 7tfaB

query
sites
7tfaB

E

E

T

L

T

G

R

Y

I

T

S

S

S

M

T

N

V

V

G

G

P

P

E
|
E

Q

P

E
|
E

Y

F

F

F

L

L

I

F

D

K

K

T

A

-

Y

-

E

-

K

-

R

-

M

-

D

-

L

-

V

-

L

-

T

-

G

-

R

-

T

-

L

-

F

-

G

D

A

E

K

K

S

G

P

D

K

P

G

T

Q

T

E

E

M

L

E

N

D

D

Q

Q

-

G

Y
|
Y

F

F

G

D

-

L

-

A

-

P

-

M

S

D

I

L

N

G

E

E

R

N

V

C

S

R

A

-

F

-

M

-

H

R

D

E

V

I

N

V

V

L

E

K

A

L

W

E

K

E

L

M

G

G

I

F

A

E

A

I

K

E

T

A

Q

S

H

H

K

H

E
|
E

V
|
V

A

A

P

P

G

G

Q

Q

Y

H

E
|
E

I

I

A

D

P

F

I

K

F

Y

S

A

V

D

T

A

N

V

V

K

A

A

T

A

D

D

H

Q

N

I

Q

Q

L

T

I

F

M

K

D

L

I

V

L

V

K

K

K

T

V

I

A

A

N

R

R

Q

H

H

G

G

L

L

V

H

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

L

A

F

G

G

V

V

N
|
N

G
|
G

S

S

G

G

K

M

H
|
H

N

C

N

N

W

Q

A

S

L

L

binding glutamine: E131 (= E195), Y153 (= Y232), E186 (= E264), G238 (= G316), H242 (= H320)
binding magnesium ion: E129 (= E193), E131 (= E195), E186 (= E264), E193 (= E271), H242 (= H320)
binding : V187 (= V265), N237 (= N315)

Sites not aligning to the query:

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
30% identity, 21% coverage: 181:325/695 of query aligns to 115:245/439 of 7tdpA

query
sites
7tdpA

E

E

T

L

T

G

R

Y

I

T

S

S

S

M

T
x
N

V

V

G
|
G

P

P

E
|
E

Q

P

E
|
E

Y

F

F

F

L

L

I

F

D

K

K

T

A

-

Y

-

E

-

K

-

R

-

M

-

D

-

L

-

V

-

L

-

T

-

G

-

R

-

T

-

L

-

F

-

G

D

A

E

K

K

S

G

P

D

K

P

G

T

Q

T

E

E

M

L

E

N

D

D

Q

Q

-

G

Y

Y

F

F

G

D

-

L

-

A

-

P

-

M

S

D

I

L

N

G

E

E

R

N

V

C

S

R

A

-

F

-

M

-

H

R

D

E

V

I

N

V

V

L

E

K

A

L

W

E

K

E

L

M

G

G

I

F

A

E

A

I

K
x
E

T

A

Q

S

H

H

K

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

Y

H

E
|
E

I

I

A
x
D

P

F

I

K

F
x
Y

S

A

V

D

T

A

N

V

V

K

A

A

T

A

D

D

H

Q

N

I

Q

Q

L

T

I

F

M

K

D

L

I

V

L

V

K

K

K

T

V

I

A

A

N

R

R

Q

H

H

G

G

L

L

V

H

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

L

A

F

G

G

V

V

N

N

G
|
G

S

S

G

G

K

M

H
|
H

N

C

N
|
N

W

Q

A
x
S

L

L

binding adenosine-5'-diphosphate: N123 (≠ T189), G125 (= G191), E127 (= E193), E179 (≠ K259), D193 (≠ A273), Y196 (≠ F276), N242 (= N322), S244 (≠ A324)
binding magnesium ion: E127 (= E193), E127 (= E193), E129 (= E195), E184 (= E264), E191 (= E271), E191 (= E271), H240 (= H320)
binding l-methionine-s-sulfoximine phosphate: E127 (= E193), E129 (= E195), E184 (= E264), E191 (= E271), G236 (= G316), H240 (= H320)

Sites not aligning to the query:

8tfkA Glutamine synthetase (see paper)
29% identity, 26% coverage: 182:359/695 of query aligns to 117:280/440 of 8tfkA

query
sites
8tfkA

E

E

T

K

T

M

R

G

I

F

S

S

S

M

T

N

V

V

G

G

P

P

E
|
E

Q

L

E
|
E

Y

F

F

F

L

L

I

F

D

-

K

-

A

-

Y

-

E

-

K

-

R

K

M

L

D

D

L

-

V

-

L

-

T

-

G

-

R

-

T

-

L

-

F

-

G

-

A

A

K

N

S

G

P

N

K

P

G

T

Q

T

E

E

M

L

E

T

D

D

Q

Q

-

G

Y

Y

F

F

G

D

-

F

S

A

I

P

N

L

E

D

R

R

V

A

S

Q

A

D

F

V

M

R

H

R

D

D

V

I

N

D

V

Y

E

A

A

L

W

E

K

H

L

M

G

G

I

F

A

Q

A

I

K

E

T

A

Q

S

H

H

K

H

E
|
E

V

V

A

A

P

P

G

S

Q

Q

Y

H

E
|
E

I

I

A
x
D

P
x
F

I

R

F
|
F

S

G

V

D

T

V

N

L

V

C

A

T

T

A

D

D

H

N

N

V

Q

V

L

T

I

F

M

K

D

Y

I

V

L

V

K

K

K

S

V

I

A

A

N

Y

R

H

H

K

G

G

L

Y

V

Y

C

A

L

S

L

F

H

M

E

P

K

K

P

P

F

L

A

F

G

G

V

V

N

N

G
|
G

S

S

G

G

K

M

H
|
H

N

S

N
|
N

W

Q

A

S

L

L

G

F

T

K

N

D

S

-

G

G

K

K

N

N

L

V

L

F

D

Y

P

D

G

P

R

D

T

T

P

P

H

T

E

K

N

L

A

S

Q

Q

-

D

F

A

L

M

T

Y

F

Y

L

I

C

G

A

G

V

L

L

L

K

K

A

H

V

I

D

R

E

E

binding adenosine-5'-diphosphate: E128 (= E193), D194 (≠ A273), F195 (≠ P274), F197 (= F276), N243 (= N322)
binding magnesium ion: E128 (= E193), E128 (= E193), E130 (= E195), E185 (= E264), E192 (= E271), E192 (= E271), H241 (= H320)
binding l-methionine-s-sulfoximine phosphate: E128 (= E193), E130 (= E195), E185 (= E264), E192 (= E271), G237 (= G316), H241 (= H320)

Sites not aligning to the query:

8ufjB Glutamine synthetase (see paper)
28% identity, 26% coverage: 182:359/695 of query aligns to 121:284/444 of 8ufjB

query
sites
8ufjB

E

E

T

K

T

M

R

G

I

F

S

S

S

M

T

N

V

V

G

G

P

P

E
|
E

Q

L

E
|
E

Y

F

F

F

L

L

I

F

D

K

-

L

K

D

A

A

Y

N

Y

G

E

N

K

P

R

T

M

T

D

E

L

L

V

T

L

D

T

Q

G

G

R

G

T

Y

L

F

F

D

G

F

A

A

K

P

S

L

P

D

K

L

G

G

Q

Q

E

D

M

V

E

R

D

-

Q

-

Y

-

F

-

G

-

S

-

I

-

N

-

E

-

R

-

V

-

S

-

A

-

F

-

M

-

H

R

D

D

V

I

N

D

V

Y

E

A

A

L

W

E

K

H

L

M

G

G

I

F

A

Q

A

I

K

E

T

A

Q

S

H

H

K

H

E
|
E

V

V

A

A

P

P

G

S

Q

Q

Y

H

E
|
E

I

I

A

D

P

F

I

R

F

F

S

G

V

D

T

V

N

L

V

C

A

T

T

A

D

D

H

N

N

V

Q

V

L

T

I

F

M

K

D

Y

I

V

L

V

K

K

K

S

V

I

A

A

N

Y

R

H

H

K

G

G

L

Y

V

Y

C

A

L

S

L

F

H

M

E

P

K

K

P

P

F

L

A

F

G

G

V

V

N

N

G

G

S

S

G

G

K

M

H
|
H

N

S

N

N

W

Q

A

S

L

L

G

F

T

K

N

D

S

-

G

G

K

K

N

N

L

V

L

F

D

Y

P

D

G

P

R

D

T

T

P

P

H

T

E

K

N

L

A

S

Q

Q

-

D

F

A

L

M

T

Y

F

Y

L

I

C

G

A

G

V

L

L

L

K

K

A

H

V

I

D

R

E

E

binding magnesium ion: E132 (= E193), E134 (= E195), E189 (= E264), E196 (= E271), H245 (= H320)

Sites not aligning to the query:

binding magnesium ion: 333

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
25% identity, 24% coverage: 190:355/695 of query aligns to 133:284/447 of 8oooA

query
sites
8oooA

V

V

G

G

P

P

E
|
E

Q

P

E

E

Y

F

F

F

L

L

I

L

D

K

K

K

A

D

Y

P

Y

H

E

N

K

P

R

H

M

K

D

Y

L

I

V

P

L

A

T

D

G

D

R

G

T

G

L

Y

F

F

G

-

A

-

K

-

S

-

P

-

K

-

G

-

Q

-

E

D

M

L

E

E

D

P

Q

M

Y

-

F

-

G

-

S

-

I

-

N

-

E

D

R

E

V

A

S
x
P

A

D

F

I

M
x
R

H
x
R

D

D

V

I

N

V

V

F

E

A

A

L

W

E

K

N

L

L

G

G

I

F

A

H

A

V

K
x
E

T

A

Q
x
S

H

H

K

H

E

E

V

V

A

A

P

P

G

G

Q

Q

Y

H

E

E

I

V

A

D

P
x
F

I
x
K

F
|
F

S

D

V

D

T

A

N

L

V

K

A

T

T

A

D

D

H

S

N

V

Q

I

L

T

I

F

M

K

D

T

I

T

L

I

K

K

K

T

V

I

A

A

N

E

R

Q

H

H

G

G

L

L

V

K

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

F

A

F

G

G

V

M

N

N

G

G

S

S

G

G

K

M

H

H

N

C

N
x
H

W

Q

A
x
S

L

I

G

W

T

L

N

N

S

G

G

E

K

P

N

S

L

F

L

Y

D

D

P

E

G

N

R

A

T

P

P

Y

H

Q

E

L

N

S

A

E

Q

T

F

C

L

M

T

N

F

Y

L

V

C

A

A

G

V

I

L

L

K

K

binding 2-oxoglutaric acid: P170 (≠ S241), R173 (≠ M244), R174 (≠ H245), S190 (≠ Q261)
binding adenosine-5'-triphosphate: E136 (= E193), E188 (≠ K259), F203 (≠ P274), K204 (≠ I275), F205 (= F276), H251 (≠ N322), S253 (≠ A324)

Sites not aligning to the query:

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
25% identity, 24% coverage: 190:355/695 of query aligns to 132:283/446 of 8ooqB

query
sites
8ooqB

V

V

G

G

P

P

E

E

Q

P

E
|
E

Y

F

F

F

L

L

I

L

D

K

K

K

A

D

Y

P

Y

H

E

N

K

P

R

H

M

K

D

Y

L

I

V

P

L

A

T

D

G

D

R

G

T

G

L

Y

F

F

G

-

A

-

K

-

S

-

P

-

K

-

G

-

Q

-

E

D

M

L

E

E

D

P

Q

M

Y

-

F

-

G

-

S

-

I

-

N

-

E

D

R

E

V

A

S
x
P

A

D

F

I

M
x
R

H
x
R

D

D

V

I

N

V

V

F

E

A

A

L

W

E

K

N

L

L

G

G

I

F

A

H

A

V

K

E

T

A

Q
x
S

H

H

K

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

Y

H

E
|
E

I

V

A

D

P

F

I

K

F

F

S

D

V

D

T

A

N

L

V

K

A

T

T

A

D

D

H

S

N

V

Q

I

L

T

I

F

M

K

D

T

I

T

L

I

K

K

K

T

V

I

A

A

N

E

R

Q

H

H

G

G

L

L

V

K

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

F

A

F

G

G

V

M

N

N

G

G

S

S

G

G

K

M

H

H

N

C

N

H

W

Q

A

S

L

I

G

W

T

L

N

N

S

G

G

E

K

P

N

S

L

F

L

Y

D

D

P

E

G

N

R

A

T

P

P

Y

H

Q

E

L

N

S

A

E

Q

T

F

C

L

M

T

N

F

Y

L

V

C

A

A

G

V

I

L

L

K

K

binding 2-oxoglutaric acid: P169 (≠ S241), R172 (≠ M244), R173 (≠ H245), S189 (≠ Q261)
binding magnesium ion: E137 (= E195), E192 (= E264), E199 (= E271)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18, 32, 86, 92

P0A9C5 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Escherichia coli (strain K12) (see 3 papers)
28% identity, 30% coverage: 162:371/695 of query aligns to 103:315/469 of P0A9C5

query
sites
P0A9C5

R

R

S

D

M

P

E

R

A

S

L

I

S

A

K

K

Q

R

A

A

V

E

R

D

I

Y

L

L

R

R

L

-

F

-

G

-

D

-

E

-

T

S

T

T

R

G

I

I

S

A

S

D

T

T

V

V

-

L

-

F

G

G

P

P

E

E

Q

P

E

E

Y

F

F

F

L

L

I

F

D

D

K

-

A

-

Y

-

E

D

K

I

R

R

M

F

D

G

L

S

V

S

L

I

T

S

G

G

R

S

T

H

L

V

F

A

G

I

A

D

K

D

S

I

P

E

K

G

G

A

Q

W

E

N

M

S

E

S

D

T

Q

Q

Y

Y

F

E

G

G

S

G

I

N

N

K

E

G

R

H

V

R

S

P

A

A

F

V

-

K

-

G

-

Y

-

F

-

P

-

V

-

P

-

V

-

D

-

S

M

A

H

Q

D

D

V

I

N

R

V

S

E

E

A

M

W

C

-

L

-

V

-

M

-

E

K

Q

L

M

G

G

I

L

A

V

K

E

T

A

Q

H

H

H

K

H

E

E

V

V

A

A

-

T

P

A

G

G

Q

Q

Y

N

E

E

I

V

A

A

P

T

I

R

F

F

S

N

V

T

T

M

N

T

V

K

A

K

T

A

D

D

H

E

N

I

Q

Q

L

I

I

Y

M

K

D

Y

I

V

L

V

K

H

K

N

V

V

A

A

N

H

R

R

H

F

G

G

L

K

V

T

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

M

A

F

G

G

V

D

N

N

G

G

S

S

G

G

K

M

H

H

N

C

N

H

W

M

A

S

L

L

G

S

T

K

N

N

S

-

G

G

K

V

N

N

L

L

L

F

D

A

P

G

G

D

R

K

T

Y

P

A

H

G

E

L

N

S

A

E

Q

Q

F

A

L

L

T

Y

F

Y

L

I

C

G

A

G

V

V

L

I

K

K

A

-

V

-

D

-

E

-

H

H

A

A

D

K

L

A

L

I

R

N

A

A

S

-

A

L

A

A

N

N

P

P

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
398 modified: O-AMP-tyrosine

P0A1P6 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
33% identity, 17% coverage: 253:371/695 of query aligns to 202:315/469 of P0A1P6

query
sites
P0A1P6

K

Q

L

M

G

G

I

L

A

V

K
x
E

T

A

Q

H

H

H

K

H

E
|
E

V

V

A

A

-

T

P

A

G

G

Q

Q

Y

N

E
|
E

I

V

A

A

P

T

I

R

F

F

S

N

V

T

T

M

N

T

V

K

A

K

T

A

D

D

H

E

N

I

Q

Q

L

I

I

Y

M

K

D

Y

I

V

L

V

K

H

K

N

V

V

A

A

N

H

R

R

H

F

G

G

L

K

V

T

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

M

A

F

G

G

V

D

N
|
N

G
|
G

S

S

G

G

K

M

H
|
H

N

C

N
x
H

W
x
M

A
x
S

L

L

G

A

T

K

N

N

S

-

G

G

K

T

N

N

L

L

L

F

D

S

P

G

G

D

R

K

T

Y

P

A

H

G

E

L

N

S

A

E

Q

Q

F

A

L

L

T

Y

F

Y

L

I

C

G

A

G

V

V

L

I

K

K

A

-

V

-

D

-

E

-

H

H

A

A

D

K

L

A

L

I

R

N

A

A

S

-

A

L

A

A

N

N

P

P

E208 (≠ K259) binding ATP
E213 (= E264) binding Mn(2+)
E221 (= E271) binding Mn(2+)
NG 265:266 (= NG 315:316) binding L-glutamate
H270 (= H320) binding Mn(2+)
HMS 272:274 (≠ NWA 322:324) binding ATP

Sites not aligning to the query:

130 binding Mn(2+)
132 binding Mn(2+)
322 binding L-glutamate
358 binding Mn(2+)
360 binding L-glutamate

2lgsA Feedback inhibition of fully unadenylylated glutamine synthetase from salmonella typhimurium by glycine, alanine, and serine (see paper)
33% identity, 17% coverage: 253:371/695 of query aligns to 195:308/445 of 2lgsA

query
sites
2lgsA

K

Q

L

M

G

G

I

L

A

V

K

E

T

A

Q

H

H

H

K

H

E
|
E

V

V

A

A

-

T

P

A

G

G

Q

Q

Y

N

E
|
E

I

V

A

A

P

T

I

R

F

F

S

N

V

T

T

M

N

T

V

K

A

K

T

A

D

D

H

E

N

I

Q

Q

L

I

I

Y

M

K

D

Y

I

V

L

V

K

H

K

N

V

V

A

A

N

H

R

R

H

F

G

G

L

K

V

T

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

M

A

F

G

G

V

D

N
|
N

G
|
G

S

S

G
|
G

K

M

H
|
H

N

C

N

H

W

M

A

S

L

L

G

A

T

K

N

N

S

-

G

G

K

T

N

N

L

L

L

F

D

S

P

G

G

D

R

K

T

Y

P

A

H

G

E

L

N

S

A

E

Q

Q

F

A

L

L

T

Y

F

Y

L

I

C

G

A

G

V

V

L

I

K

K

A

-

V

-

D

-

E

-

H

H

A

A

D

K

L

A

L

I

R

N

A

A

S

-

A

L

A

A

N

N

P

P

binding glutamic acid: N258 (= N315), G259 (= G316), G261 (= G318), H263 (= H320)
binding manganese (ii) ion: E206 (= E264), E214 (= E271), H263 (= H320)

Sites not aligning to the query:

1lgrA Interactions of nucleotides with fully unadenylylated glutamine synthetase from salmonella typhimurium (see paper)
33% identity, 17% coverage: 253:371/695 of query aligns to 195:308/445 of 1lgrA

query
sites
1lgrA

K

Q

L

M

G

G

I

L

A

V

K
x
E

T

A

Q

H

H

H

K

H

E
|
E

V

V

A

A

-

T

P

A

G

G

Q

Q

Y

N

E
|
E

I

V

A

A

P
x
T

I

R

F
|
F

S

N

V

T

T

M

N

T

V

K

A

K

T

A

D

D

H

E

N

I

Q

Q

L

I

I

Y

M

K

D

Y

I

V

L

V

K

H

K

N

V

V

A

A

N

H

R

R

H

F

G

G

L

K

V

T

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

M

A

F

G

G

V

D

N

N

G

G

S

S

G

G

K

M

H
|
H

N

C

N
x
H

W

M

A
x
S

L

L

G

A

T

K

N

N

S

-

G

G

K

T

N

N

L

L

L

F

D

S

P

G

G

D

R

K

T

Y

P

A

H

G

E

L

N

S

A

E

Q

Q

F

A

L

L

T

Y

F

Y

L

I

C

G

A

G

V

V

L

I

K

K

A

-

V

-

D

-

E

-

H

H

A

A

D

K

L

A

L

I

R

N

A

A

S

-

A

L

A

A

N

N

P

P

binding adenosine monophosphate: E201 (≠ K259), T217 (≠ P274), F219 (= F276), H265 (≠ N322), S267 (≠ A324)
binding manganese (ii) ion: E206 (= E264), E214 (= E271), H263 (= H320)

Sites not aligning to the query:

1fpyA Crystal structure of glutamine synthetase from salmonella typhimurium with inhibitor phosphinothricin (see paper)
33% identity, 17% coverage: 253:371/695 of query aligns to 201:314/468 of 1fpyA

query
sites
1fpyA

K

Q

L

M

G

G

I

L

A

V

K
x
E

T

A

Q

H

H

H

K

H

E
|
E

V

V

A

A

-

T

P

A

G

G

Q

Q

Y

N

E
|
E

I

V

A

A

P
x
T

I

R

F
|
F

S

N

V

T

T

M

N

T

V

K

A

K

T

A

D

D

H

E

N

I

Q

Q

L

I

I

Y

M

K

D

Y

I

V

L

V

K

H

K

N

V

V

A

A

N

H

R

R

H

F

G

G

L

K

V

T

C

A

L

T

L

F

H

M

E

P

K

K

P

P

F

M

A

F

G

G

V

D

N

N

G
|
G

S

S

G

G

K

M

H
|
H

N

C

N
x
H

W

M

A
x
S

L

L

G

A

T

K

N

N

S

-

G

G

K

T

N

N

L

L

L

F

D

S

P

G

G

D

R

K

T

Y

P

A

H

G

E

L

N

S

A

E

Q

Q

F

A

L

L

T

Y

F

Y

L

I

C

G

A

G

V

V

L

I

K

K

A

-

V

-

D

-

E

-

H

H

A

A

D

K

L

A

L

I

R

N

A

A

S

-

A

L

A

A

N

N

P

P

binding adenosine-5'-diphosphate: E207 (≠ K259), T223 (≠ P274), F225 (= F276), H271 (≠ N322), S273 (≠ A324)
binding manganese (ii) ion: E212 (= E264), E220 (= E271), H269 (= H320)
binding phosphinothricin: E212 (= E264), G265 (= G316), H269 (= H320)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 127, 129, 355, 357
binding manganese (ii) ion: 129, 131, 357
binding phosphinothricin: 131, 321, 327, 359

Query Sequence

>WP_015943100.1 NCBI__GCF_000021925.1:WP_015943100.1
MKIFGMNVFNDNVMRERLPKNIYKSLKNTIDNGLPLEPSVAEVVASAMKDWAIEKGATHY
THWFQPLTGLTAEKHDSFIAPTNDGRVIMEFSGKELIKGEPDASSFPNGGIRSTFEARGY
TAWDCTSPAFLREEGTNIVLCIPTAFCAYTGEALDNKTPLLRSMEALSKQAVRILRLFGD
EETTRISSTVGPEQEYFLIDKAYYEKRMDLVLTGRTLFGAKSPKGQEMEDQYFGSINERV
SAFMHDVNVEAWKLGIAAKTQHKEVAPGQYEIAPIFSVTNVATDHNQLIMDILKKVANRH
GLVCLLHEKPFAGVNGSGKHNNWALGTNSGKNLLDPGRTPHENAQFLTFLCAVLKAVDEH
ADLLRASAANPGNDHRLGANEAPPAIISVFLGDQLSDILAQLQNGGLTHSIAESRMNIGV
PTLPVLRKDSTDRNRTSPFAFTGNKFEFRMVPSSLSIAGPNIVLNSIVAEALCQMADRLE
QAEDFNADLRKLLKEIAVKHSRIVFDGNNYSGEWVEEAERRGLPNIRSTVDAIPAWVTEK
AVALFEKHGVLSGTELHSRYEILLEQYNKIINIEAQTMLYMVKRQIMPAGLKMIQNLATG
IAAVKAVSGALVSVQERLLNQVSSVMVAVDESLQALEGKTNEALAMGDVFEQGVFYRDEV
FTAMNTLRAAVDQLEELVDEEYWPLPSYADMLFKL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory