SitesBLAST

R52 (≠ L53) mutation to H: Shows increased expression and forms larger colonies.
H86 (≠ Y86) mutation to R: Can be bound and inactivated by MtfA.
F136 (vs. gap) mutation to A: Decreases association with PtsG EIIB domain.
H247 (= H248) binding Zn(2+)
C257 (= C258) binding Zn(2+); mutation to A: Strongly reduced activity; when associated with A-259.; mutation to S: Strongly reduced activity; when associated with S-259.
C259 (= C260) binding Zn(2+); mutation to A: Strongly reduced activity; when associated with A-257.; mutation to S: Strongly reduced activity; when associated with S-257.
C264 (= C265) binding Zn(2+)
R306 (≠ E309) mutation to G: Forms dimers but not tetramers; when associated with G-310.
L310 (≠ I313) mutation to G: Forms dimers but not tetramers; when associated with G-306.

5f7rA Rok repressor lmo0178 from listeria monocytogenes bound to inducer (see paper)
24% identity, 78% coverage: 86:400/406 of query aligns to 2:305/306 of 5f7rA

query
sites
5f7rA

Y

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binding alpha-D-glucopyranose: K7 (≠ D91), E10 (≠ R94), G70 (= G154), N110 (≠ G196), N110 (≠ G196), S134 (≠ N220), V135 (≠ C221), G138 (= G224), L139 (≠ I225), G140 (≠ R226), E159 (≠ T245), H162 (= H248), E181 (= E267), E253 (≠ P346), W293 (≠ S388)
binding zinc ion: H162 (= H248), C172 (= C258), C174 (= C260), C179 (= C265)

2yhwA High-resolution crystal structures of n-acetylmannosamine kinase: insights about substrate specificity, activity and inhibitor modelling. (see paper)
32% identity, 51% coverage: 145:350/406 of query aligns to 63:266/309 of 2yhwA

query
sites
2yhwA

V

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H

binding calcium ion: N112 (= N195), N115 (= N198), G144 (≠ T227), A161 (≠ D244)
binding zinc ion: H165 (= H248), C175 (= C258), C177 (= C260), C182 (= C265)

Q9Y223 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Homo sapiens (Human) (see 18 papers)
32% identity, 51% coverage: 145:350/406 of query aligns to 467:674/722 of Q9Y223

query
sites
Q9Y223

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H

I472 (= I150) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 50% of the wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to less than 10% of wild-type activity
G476 (= G154) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
R477 (≠ P155) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
T489 (≠ E167) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
N516 (= N195) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
D517 (≠ G196) active site; binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate; mutation D->A,N: Loss of N-acylmannosamine kinase activity. Decreased affinity for N-acyl-D-mannosamine. No effect on structure.
N519 (= N198) to S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs1554658910; mutation to S: Decreased N-acylmannosamine kinase activity.
A524 (= A203) to V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 10% of wild-type activity; decreased N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs764698870
F528 (≠ Y207) to C: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70% of wild-type activity; decreased N-acylmannosamine kinase activity; dbSNP:rs986773986; mutation to C: Decreased N-acylmannosamine kinase activity.
G545 (= G224) binding an N-acyl-D-mannosamine 6-phosphate
E566 (≠ T245) binding an N-acyl-D-mannosamine
H569 (= H248) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate; binding Zn(2+)
V572 (≠ I251) to L: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70-80% of wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to less than 10% of wild-type activity; does not affect homohexamers formation; dbSNP:rs121908632
G576 (= G255) to E: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs121908625
C579 (= C258) binding Zn(2+)
C581 (= C260) binding Zn(2+)
C586 (= C265) binding Zn(2+)
I587 (≠ V266) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs748949603; mutation to T: Decreased N-acylmannosamine kinase activity.
E588 (= E267) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
A630 (≠ E306) to T: in NM; decreased N-acylmannosamine kinase activity; does not affect homohexamers formation; dbSNP:rs1382191649
A631 (= A307) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 80% of wild-type activity; decreased N-acylmannosamine kinase activity; retains 75% of wild-type activity; dbSNP:rs121908626; to V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70% of wild-type activity; decreased N-acylmannosamine kinase activity; does not affect homohexamers formation; dbSNP:rs62541771; mutation A->V,T: Decreased N-acylmannosamine kinase activity.

Sites not aligning to the query:

13 C → S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; dbSNP:rs1209266607
19 binding UDP
23 binding UDP
113 binding UDP
132 H → Q: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 10% of wild-type activity; impaired homohexamers formation
176 D → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs139425890
177 R → C: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs539332585
200 I → F: in NM; uncertain significance; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 90% of wild-type activity; decreased N-acylmannosamine kinase activity; retains 75% of wild-type activity; dbSNP:rs369328625
206 G → S: in NM; moderate phenotype with unusual involvement of quadriceps; dbSNP:rs766266918
220 binding UDP
253 binding UDP
259 binding CMP-N-acetyl-beta-neuraminate
263 R → L: in SIALURIA; strong reduction of feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908623
266 R → Q: in SIALURIA; abolishes feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908622; R → W: in sialuria; dbSNP:rs121908621
271 binding CMP-N-acetyl-beta-neuraminate
280 binding CMP-N-acetyl-beta-neuraminate
281 binding CMP-N-acetyl-beta-neuraminate
282 binding UDP
301 binding UDP
302 binding UDP
303 C → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 80% of wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to 60% of wild-type activity; requires 2 nucleotide substitutions; dbSNP:rs121908633
307 binding UDP
321 binding UDP
331 V → A: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation
378 D → Y: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 10-30% of wild-type activity; decreased N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs199877522
413 binding Mg(2+)
416 binding an N-acyl-D-mannosamine 6-phosphate
417 binding ADP
418 binding ADP
420 binding ADP
708 G → S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; severely decreased; dbSNP:rs1554657922
712 M→T: Decreased N-acylmannosamine kinase activity.

2yi1A Crystal structure of n-acetylmannosamine kinase in complex with n- acetyl mannosamine 6-phosphate and adp. (see paper)
31% identity, 51% coverage: 145:350/406 of query aligns to 63:265/308 of 2yi1A

query
sites
2yi1A

V

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D

A

E

D

D

-

L

-

V

-

E

-

G

-

M

R

S

P

A

V

V

S

G

Y

A

L
|
L

E

H

L

L

C

I

R

Q

E

A

A

A

E

K

E

L

N

G

D

N

T

A

I

K

A

A

R

Q

Q

S

V

I

L

L

Q

R

N

T

A

A

A

G

V

T

R

A

M

L

G

G

T

L

G

G

L

V

A

V

N

N

F

I

I

L

Q

H

L

T

L

M

N

N

P

P

G

S

L

L

V

V

V

I

L

L

S

S

G

G

P
x
V

L

L

I

A

L

S

H

H

binding adenosine-5'-diphosphate: T140 (≠ V223), G189 (≠ I272), L216 (= L301), V261 (≠ P346)
binding 2-acetamido-2-deoxy-6-O-phosphono-alpha-D-mannopyranose: G71 (≠ V153), G72 (= G154), R73 (≠ P155), S84 (≠ P166), T85 (≠ E167), L87 (≠ F169), N112 (= N195), D113 (≠ G196), G139 (= G222), T140 (≠ V223), G141 (= G224), I142 (= I225), E162 (≠ T245), H165 (= H248), E184 (= E267)
binding calcium ion: N112 (= N195), N115 (= N198), G144 (≠ T227), A161 (≠ D244)
binding zinc ion: H165 (= H248), C175 (= C258), C177 (= C260), C182 (= C265)

Sites not aligning to the query:

2yhyA Structure of n-acetylmannosamine kinase in complex with n- acetylmannosamine and adp (see paper)
31% identity, 51% coverage: 145:350/406 of query aligns to 63:265/308 of 2yhyA

query
sites
2yhyA

V

I

I

L

G

G

V

V

G

G

I

I

G

S

T

T

V

G

G

G

P

R

L

V

D

N

R

P

Q

R

S

E

G

G

I

I

I

V

L

L

N

H

P

S

E

T

N

K

F

L

E

-

A

I

Q

Q

G

E

W

W

E

N

N

S

I

V

P

D

L

L

K

R

A

T

I

P

V

L

E

S

E

D

R

T

T

L

G

H

L

L

P

P

V

V

I

W

I

V

D

D

N
|
N

G

D

A

G

N
|
N

G

C

A

A

V

A

L

L

A

A

E

E

T

R

R

K

Y

F

G

G

S

Q

G

G

K

K

G

G

M

L

K

E

S

N

V

F

I

V

Y

T

L

L

N

I

C

T

G

G

V
x
T

G

G

I

I

R

G

T
x
G

G

G

V

I

I

I

S

H

S

Q

G

H

T

E

L

L

V

I

R

H

T

G

I

S

N

S

D

F

A

C

D

A

D
x
A

T

E

F

L

A

G

H
|
H

M

L

V

V

I

V

D

S

V

L

N

D

G

G

K

P

P

D

C
|
C

H

S

C
|
C

G

G

N

S

Q

H

G

G

C
|
C

V

I

E

E

R

A

Y

Y

S

A

S

S

I
x
G

Y

M

A

A

I

L

M

-

E

-

A

-

L

-

A

-

E

-

M

-

P

-

Q

Q

G

R

K

E

D

A

R

K

N

L

H

H

K

D

A

E

D

D

-

L

-

V

-

E

-

G

-

M

R

S

P

A

V

V

S

G

Y

A

L
|
L

E

H

L

L

C

I

R

Q

E

A

A

A

E

K

E

L

N

G

D

N

T

A

I

K

A

A

R

Q

Q

S

V

I

L

L

Q

R

N

T

A

A

A

G

V

T

R

A

M

L

G

G

T

L

G

G

L

V

A

V

N

N

F

I

I

L

Q

H

L

T

L

M

N

N

P

P

G

S

L

L

V

V

V

I

L

L

S

S

G

G

P
x
V

L

L

I

A

L

S

H

H

binding adenosine-5'-diphosphate: T140 (≠ V223), G189 (≠ I272), L216 (= L301), V261 (≠ P346)
binding calcium ion: N112 (= N195), N115 (= N198), G144 (≠ T227), A161 (≠ D244)
binding zinc ion: H165 (= H248), C175 (= C258), C177 (= C260), C182 (= C265)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 11, 12, 13, 14, 16

O35826 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Rattus norvegicus (Rat) (see paper)
32% identity, 51% coverage: 145:350/406 of query aligns to 467:674/722 of O35826

query
sites
O35826

V
x
I

I
x
L

G
|
G

V
|
V

G
|
G

I
|
I

G
x
S

T
|
T

V
x
G

G
|
G

P
x
R

L
x
V

D
x
N

R
x
P

Q
|
Q

S
x
E

G
|
G

I
x
V

L
|
L

N
x
H

P
x
S

E
x
T

N
x
K

F
x
L

E

-

A
x
I

Q
|
Q

G
x
E

W
|
W

E
x
N

N
x
S

I
x
V

P
x
D

L
|
L

K
x
R

A
x
T

I
x
P

V
x
L

E
x
S

E
x
D

R
x
T

T
x
L

G
x
H

L
|
L

P
|
P

V
|
V

I
x
W

I
x
V

D
|
D

N
|
N

G
x
D

A
x
G

N
|
N

G
x
C

A
|
A

V
x
A

L
x
M

A
|
A

E
|
E

T
x
R

R
x
K

Y
x
F

G
|
G

S
x
Q

G
|
G

K
|
K

G
|
G

M
x
Q

K
x
E

S
x
N

V
x
F

I
x
V

Y
x
T

L
|
L

N
x
I

C
x
T

G
|
G

V
x
T

G
|
G

I
|
I

R
x
G

T
x
G

G
|
G

V
x
I

I
|
I

S
x
H

S
x
Q

G
x
H

T
x
E

L
|
L

V
x
I

R
x
H

T
x
G

I
x
S

N
x
S

D
x
F

A
x
C

D
x
A

T
x
E

F
x
L

A
x
G

H
|
H

M
x
L

V
|
V

I
x
V

D
x
S

V
x
L

N
x
D

G
|
G

K
x
P

P
x
D

C
|
C

H
x
S

C
|
C

G
|
G

N
x
S

Q
x
H

G
|
G

C
|
C

V
x
I

E
|
E

R
x
A

Y
|
Y

S
x
A

S
|
S

I
x
G

Y
x
M

A
|
A

I
x
L

M
x
Q

-
x
R

-
x
E

-
x
A

E
x
K

A
x
K

L
|
L

A
x
H

E
x
D

E
|
E

M
x
D

P
x
L

Q
x
L

G
x
L

K
x
V

D
x
E

R
x
G

R
x
M

N
x
S

H
x
V

K
x
P

A
x
K

D
|
D

R
x
E

P
x
A

V
|
V

S
x
G

Y
x
A

L
|
L

E
x
H

L
|
L

C
x
I

R
x
Q

E
x
A

A
|
A

E
x
K

E
x
L

N
x
G

D
x
N

T
x
V

I
x
K

A
|
A

R
x
Q

Q
x
S

V
x
I

L
|
L

Q
x
R

N
x
T

A
|
A

A
x
G

V
x
T

R
x
A

M
x
L

G
|
G

T
x
L

G
|
G

L
x
V

A
x
V

N
|
N

F
x
I

I
x
L

Q
x
H

L
x
T

L
x
M

N
|
N

P
|
P

G
x
S

L
|
L

V
|
V

V
x
I

L
|
L

S
|
S

G
|
G

P
x
V

L
|
L

I
x
A

L
x
S

H
|
H

Sites not aligning to the query:

1 UDP-N-acetylglucosamine 2-epimerase
49 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Does not interfere with enzyme oligomerization.
110 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
132 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
155 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
157 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
406:722 N-acetylmannosamine kinase
413 mutation D->K,N: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.
420 R→M: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.

1z6rA Crystal structure of mlc from escherichia coli (see paper)
23% identity, 85% coverage: 19:365/406 of query aligns to 7:338/382 of 1z6rA

query
sites
1z6rA

A

A

K

G

K

A

I

V

L

Y

G

R

L

L

I

I

L

D

H

Q

K

L

G

G

G

P

M

V

T

S

K

R

S

I

Q

D

L

L

A

S

K

R

V

L

A

A

G

Q

L

L

K

A

L

P

T

A

T

S

L

I

N

T

R

K

M

I

M

V

L

H

P

E

L

M

E

L

K

E

A

A

D

H

L

L

I

V

L

-

P

-

T

Q

E

E

T

L

G

G

E

-

S

-

T

-

G

-

R

-

K

-

P

-

V

-

I

-

Y

L

D

V

V

V

N

E

P

T

R

E

R

A

Y

W

Y

H

V

Y

I

L

G

S

I

L

D

R

I

I

S

S

R

R

L

G

Y

E

S

I

Q

F

V

L

V

A

L

L

T

R

N

D

L

L

K

S

M

S

E

K

L

L

I

V

E

V

K

E

D

E

R

S

F

Q

D

E

M

L

D

A

-

L

R

K

N

D

S

D

S

L

P

P

Q

-

A

-

T

L

L

L

N

D

R

R

I

I

L

I

D

S

W

H

I

I

G

D

R

Q

-

F

V

I

M

R

E

H

K

Q

R

K

G

K

H

L

G

E

Y

R

V

L

I

T

G

S

V

I

G

A

I

I

G

T

T

L

V

P

G

G

P

I

L

I

D

D

R

T

Q

E

S

N

G

G

I

I

I

V

L

H

N

R

P

M

E

P

N

F

F

Y

E

E

A

-

Q

-

G

D

W

V

E

K

N

E

I

M

P

P

L

L

K

G

A

E

I

A

V

L

E

E

E

Q

R

H

T

T

G

G

L

V

P

P

V

V

I

Y

I

I

D

Q

N

H

G

D

A

I

N

S

G

A

A

W

V

T

L

M

A

A

E

E

T

A

R

L

Y

F

G

G

S

A

G

S

K

R

G

G

M

A

K

R

S

D

V

V

I

I

Y

Q

L

V

N

V

C

I

G

D

V

H

G

N

I

V

R

G

T

A

G

G

V

V

I

I

S

T

S

D

G

G

T

H

L

L

V

L

R

H

T

A

I

G

N

S

D

S

A

S

D

L

D

V

T

E

F

I

A

G

H
|
H

M

T

V

Q

I

V

D

D

V

P

N

Y

G

G

K

K

P

R

C
|
C

H

Y

C
|
C

G

G

N

N

Q

H

G

G

C
|
C

V

L

E

E

R

T

Y

I

S

A

S

S

I

V

Y

D

A

S

I

I

M

L

E

E

A

L

L

A

A

Q

E

L

E

R

M

L

P

N

Q

Q

G

S

K

M

D

S

R

S

R

M

N

L

H

H

K

G

A

-

D

-

R

Q

P

P

V

L

S

T

Y

V

L

D

E

S

L

L

C

C

R

Q

E

A

A

A

E

L

E

R

N

G

D

D

T

L

I

L

A

A

R

K

Q

D

V

I

L

I

Q

T

N

G

A

V

A

G

V

A

R

H

M

V

G

G

T

R

G

I

L

L

A

A

N

I

F

M

I

V

Q

N

L

L

L

F

N

N

P

P

G

Q

L

K

V

I

V

L

L

I

S

G

G

S

P

P

L

L

I

S

L

K

H

A

S

A

Q

D

L

I

F

L

Y

F

E

P

V

V

C

I

V

S

E

D

A

S

A

I

K

R

R

Q

binding zinc ion: H223 (= H248), C233 (= C258), C235 (= C260), C240 (= C265)

1z05A Crystal structure of the rok family transcriptional regulator, homolog of e.Coli mlc protein.
24% identity, 84% coverage: 19:359/406 of query aligns to 8:349/396 of 1z05A

query
sites
1z05A

A

A

K

G

K

R

I

V

L

Y

G

K

L

L

I

I

L

D

H

Q

K

K

G

G

G

P

M

I

T

S

K

R

S

I

Q

D

L

L

A

S

K

K

V

E

A

S

G

E

L

L

K

A

L

P

T

A

T

S

L

I

N

T

R

K

M

I

M

T

L

R

P

E

L

L

E

I

K

D

A

A

D

H

L

L

I

I

L

H

P

E

T

T

E

T

T

V

G

Q

E

E

S

A

T

I

G

S

-

R

G

G

R

R

K

P

P

A

V

V

I

G

Y

L

D

Q

V

T

N

N

P

N

R

L

R

G

Y

W

Y

Q

V

F

I

L

G

S

I

M

D

R

I

L

S

G

R

R

L

G

Y

Y

S

L

Q

T

V

I

V

A

L

L

T

H

N

E

L

L

K

G

M

G

E

E

L

V

I

L

E

I

K

D

D

T

R

K

F

I

D

D

M

I

D

-

R

H

N

E

S

I

S

D

P

Q

Q

D

A

D

T

V

L

L

N

A

R

R

I

L

L

L

D

F

W

E

I

I

G

E

R

E

V

F

M

F

E

Q

K

T

R

Y

G

A

H

A

G

Q

Y

L

-

D

-

R

V

V

I

T

G

S

V

I

G

A

I

I

G

T

T

L

V

P

G

G

P

L

L

V

D

N

R

S

Q

E

S

Q

G

G

I

I

I

V

L

L

N

Q

P

M

E

P

N

H

F

Y

E

N

A

V

Q

K

G

-

W

-

E

-

N

N

I

L

P

A

L

L

K

G

A

P

I

E

V

I

E

Y

E

K

R

A

T

T

G

G

L

L

P

P

V

V

I

F

I

V

D

A

N

N

G

D

A

T

N

R

G

A

A

W

V

A

L

L

A

A

E

E

T

K

R

L

Y

F

G

G

S

H

G

S

K

Q

G

D

M

V

K

D

S

N

V

S

I

V

Y

L

L

I

N

S

C

I

G

H

V

H

G

G

I

L

R

G

T

A

G

G

V

I

I

V

S

L

S

D

G

G

T

R

L

V

V

L

R

Q

T

G

I

R

N

H

D

G

A

N

D

I

D

G

T

E

F

L

A

G

H
|
H

M

I

V

Q

I

I

D

D

V

P

N

Q

G

G

K

K

P

R

C
|
C

H

H

C
|
C

G

G

N

N

Q

Y

G

G

C
|
C

V

L

E

E

R

T

Y

V

S

A

S

S

I

S

Y

Q

A

A

I

I

M

R

E

D

A

Q

L

V

A

T

E

A

E

R

M

I

P

Q

Q

A

G

G

K

E

D

P

R

S

R

C

N

L

H

A

K

T

A

V

D

E

R

E

P

-

V

I

S

S

Y

I

L

E

E

D

L

I

C

C

R

A

E

A

A

A

E

A

E

D

N

G

D

D

T

P

I

L

A

A

R

V

Q

D

V

V

L

I

Q

Q

N

Q

A

L

A

G

V

R

R

Y

M

L

G

G

T

A

G

A

L

I

A

A

N

I

F

V

I

I

Q

N

L

L

L

F

N

N

P

P

G

E

L

K

V

I

V

L

L

I

S

G

G

G

P

V

L

I

I

N

L

Q

H

A

S

K

Q

S

L

I

F

L

Y

Y

-

P

-

S

-

I

E

E

V

Q

C

C

V

I

binding zinc ion: H236 (= H248), C246 (= C258), C248 (= C260), C253 (= C265)

3vovB Crystal structure of rok hexokinase from thermus thermophilus (see paper)
33% identity, 53% coverage: 148:364/406 of query aligns to 61:263/298 of 3vovB

query
sites
3vovB

V

I

G

G

I

L

G

G

T

T

V

P

G

G

P

P

L

L

D

D

R

F

Q

R

S

R

G

G

I

V

I

I

L

-

N

-

P

-

E

-

N

R

F

P

E

N

A

I

Q

P

G

G

W

V

E

Q

N

D

I

F

P

P

L

I

K

R

A

R

I

I

V

L

E

E

R

A

T

T

G

G

L

R

P

P

V

V

I

F

I

L

D

E

N

N

G

D

A

A

N

N

G

A

A

A

V

A

L

L

A

A

E

E

T

H

R

H

Y

L

G

G

S

A

G

A

K

Q

G

G

M

E

K

E

S

S

V

S

I

L

Y

Y

L

L

N

T

C

V

G

S

V

T

G

G

I

I

R

G

T

G

G

G

V

V

I

V

S

L

S

G

G

G

T

R

L

V

V

L

R

R

T

G

I

E

N

R

D

G

A

Q

D

G

T

E

F

L

A

G

H
|
H

M

L

V

T

I

L

D

L

V

P

N

G

G

G

K

P

P

A

C
|
C

H

G

C
|
C

G

G

N

L

Q

E

G

G

C
|
C

V

-

E

-

R

-

Y

-

S

-

I

-

Y

-

A

-

I

-

M

L

E

E

A

A

L

L

A

A

E

A

E

G

M

R

P

A

Q

L

G

E

K

R

D

D

R

-

R

A

N

T

H

Y

K

A

A

F

D

Q

R

R

P

P

V

V

S

D

Y

T

L

R

E

E

L

L

C

F

R

R

E

L

A

F

E

Q

E

A

N

G

D

D

T

P

I

K

A

A

R

E

Q

R

V

L

L

V

Q

L

N

Q

A

A

V

R

R

Y

M

V

G

G

T

I

G

G

L

L

A

A

N

S

F

L

I

V

Q

K

L

A

L

F

N

D

P

P

G

G

L

V

V

V

L

L

S

G

G

G

P

G

L

V

I

A

L

L

H

N

S

A

-

P

Q

E

L

G

F

Y

Y

W

E

E

V

A

C

L

V

L

E

E

A

A

A

Y

K

R

R

R

binding zinc ion: H157 (= H248), C167 (= C258), C169 (= C260), C174 (= C265)

3vglA Crystal structure of a rok family glucokinase from streptomyces griseus in complex with glucose and amppnp (see paper)
30% identity, 57% coverage: 125:356/406 of query aligns to 35:270/312 of 3vglA

query
sites
3vglA

T

T

L

A

N

E

R

G

I

I

L

V

D

D

W

A

I

I

G

C

R

A

V

A

M

V

E

A

K

G

R

A

G

S

H

E

G

G

Y

H

-

D

V

V

I

E

G

A

V

V

G

G

I

I

G

G

T

A

V

A

G
|
G

P

Y

L

V

D

D

R

D

Q

K

S

R

G

A

I

T

I

V

L

L

N

F

P

A

E
x
P

N

N

F

I

E

D

A

-

Q

-

G

-

W

W

E

R

N

H

I

E

P

P

L

L

K

K

A

D

I

K

V

V

E

E

E

Q

R

R

T

V

G

G

L

L

P

P

V

V

I

V

D

E

N
|
N

G
x
D

A

A

N

N

G

A

A

A

V

A

L

W

A

G

E

E

T

Y

R

R

Y

F

G

G

S

A

G

G

K

Q

G

G

M

H

K

D

S

D

V

V

I

I

Y

C

L

I

N

T

C

L

G
|
G

V
x
T

G

G

I
x
L

R
x
G

T

G

G

G

V

I

I

I

S

I

S

G

G

N

T

K

L

L

V

R

R

R

T

G

I

R

N

F

D

G

A

V

D

A

T
x
E

F

F

A

G

H
|
H

M

I

V

R

I

V

D

V

V

P

N

D

G

G

K

L

P

L

C
|
C

H

G

C
|
C

G

G

N

S

Q

Q

G

G

C
|
C

V

W

E
|
E

R

Q

Y

Y

S

A

S

S

I
x
G

Y

R

A

A

I

L

M

V

-

R

-

Y

-

A

E

K

A

Q

L

R

A

A

E

N

E

A

M

T

P

P

Q

E

G

N

K

A

D

A

R

V

R

L

N

L

H

G

K

L

A

G

D

D

R

G

P

S

V

V

S

D

Y

G

L

I

E

E

-
x
G

-

K

-

H

L

I

C
x
S

R

E

E

A

A

A

E

R

E

Q

N

G

D

D

T

P

I

V

A

A

R

V

Q

D

V

S

L

F

Q

R

N

E

A

L

A

A

V

R

R

W

M

A

G

G

T

A

G

G

L

L

A

A

N

D

F

L

I

A

Q

S

L

L

L

F

N

D

P

P

G

S

L

A

V

F

V

I

L

V

S

G

G

G

P
x
G

L
x
V

I

S

L

D

H
x
E

S

G

Q

E

L

L

F

V

Y

L

E

D

binding phosphoaminophosphonic acid-adenylate ester: G130 (= G222), T131 (≠ V223), G180 (≠ I272), G214 (vs. gap), S218 (≠ C304), G260 (≠ P346), V261 (≠ L347), E264 (≠ H350)
binding beta-D-glucopyranose: G65 (= G154), P78 (≠ E167), N103 (= N195), D104 (≠ G196), L133 (≠ I225), G134 (≠ R226), E153 (≠ T245), H156 (= H248), E175 (= E267)
binding zinc ion: H156 (= H248), C166 (= C258), C168 (= C260), C173 (= C265)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 9, 11, 12

3vgkB Crystal structure of a rok family glucokinase from streptomyces griseus (see paper)
30% identity, 57% coverage: 125:356/406 of query aligns to 35:270/312 of 3vgkB

query
sites
3vgkB

T

T

L

A

N

E

R

G

I

I

L

V

D

D

W

A

I

I

G

C

R

A

V

A

M

V

E

A

K

G

R

A

G

S

H

E

G

G

Y

H

-

D

V

V

I

E

G

A

V

V

G

G

I

I

G

G

T

A

V

A

G

G

P

Y

L

V

D

D

R

D

Q

K

S

R

G

A

I

T

I

V

L

L

N

F

P

A

E

P

N

N

F

I

E

D

A

-

Q

-

G

-

W

W

E

R

N

H

I

E

P

P

L

L

K

K

A

D

I

K

V

V

E

E

E

Q

R

R

T

V

G

G

L

L

P

P

V

V

I

V

D

E

N

N

G

D

A

A

N

N

G

A

A

A

V

A

L

W

A

G

E

E

T

Y

R

R

Y

F

G

G

S

A

G

G

K

Q

G

G

M

H

K

D

S

D

V

V

I

I

Y

C

L

I

N

T

C

L

G

G

V

T

G

G

I

L

R

G

T

G

G

G

V

I

I

I

S

I

S

G

G

N

T

K

L

L

V

R

R

R

T

G

I

R

N

F

D

G

A

V

D

A

T

E

F

F

A

G

H
|
H

M

I

V

R

I

V

D

V

V

P

N

D

G

G

K

L

P

L

C
|
C

H

G

C
|
C

G

G

N

S

Q

Q

G

G

C
|
C

V

W

E

E

R

Q

Y

Y

S

A

S

S

I

G

Y

R

A

A

I

L

M

V

-

R

-

Y

-

A

E

K

A

Q

L

R

A

A

E

N

E

A

M

T

P

P

Q

E

G

N

K

A

D

A

R

V

R

L

N

L

H

G

K

L

A

G

D

D

R

G

P

S

V

V

S

D

Y

G

L

I

E

E

-

G

-

K

-

H

L

I

C

S

R

E

E

A

A

A

E

R

E

Q

N

G

D

D

T

P

I

V

A

A

R

V

Q

D

V

S

L

F

Q

R

N

E

A

L

A

A

V

R

R

W

M

A

G

G

T

A

G

G

L

L

A

A

N

D

F

L

I

A

Q

S

L

L

L

F

N

D

P

P

G

S

L

A

V

F

V

I

L

V

S

G

G

G

P

G

L

V

I

S

L

D

H

E

S

G

Q

E

L

L

F

V

Y

L

E

D

binding zinc ion: H156 (= H248), C166 (= C258), C168 (= C260), C173 (= C265)

2qm1B Crystal structure of glucokinase from enterococcus faecalis
30% identity, 49% coverage: 146:345/406 of query aligns to 69:270/325 of 2qm1B

query
sites
2qm1B

I

V

G

G

V

I

G

G

I

M

G

G

T

T

V

P

G

G

P

S

L

V

D

D

R

I

Q

E

S

K

G

G

I

T

I

V

L

V

N

G

P

A

E

Y

N

N

F

L

E

N

A

-

Q

-

G

-

W

W

E

T

N

T

I

V

-

Q

P

P

L

V

K

K

A

E

I

Q

V

I

E

E

E

S

R

A

T

L

G

G

L

I

P

P

V

F

I

A

I

L

D

D

N
|
N

G

D

A

A

N
|
N

G

V

A

A

V

A

L

L

A

G

E

E

T

R

R

W

Y

K

G

G

S

A

G

G

K

E

G

N

M

N

K

P

S

D

V

V

I

I

Y

F

L

I

N

T

C

L

G

G

V

T

G

G

I

V

R

G

T
x
G

G

G

V

I

I

V

S

A

G

G

T

K

L

L

V

L

R

H

T

G

I

V

N

A

D

G

A

C

D

A

D

G

T

E

F

V

A

G

H
|
H

M

V

V

T

I

V

D

D

V

P

N

N

G

G

K

F

P

D

C
|
C

H

T

C
|
C

G

G

N

K

Q

R

G

G

C
|
C

V

L

E

E

R

T

Y

V

S

S

I

A

Y

T

A

G

I

V

M

V

E

R

A

V

-

A

-

R

-

H

L

L

A

S

E

E

M

F

P

A

Q

G

G

D

K

S

D

E

R

L

R

K

N

Q

H

A

K

I

A

D

D

D

-

G

R

Q

P

D

V

V

S

S

Y

S

L

K

E

D

L

V

C

F

R

E

E

F

A

A

E

E

E

K

N

G

D

D

T

H

I

F

A

A

R

L

Q

M

V

V

L

V

Q

D

N

R

A

V

A

C

V

F

R

Y

M

L

G

G

T

L

G

A

L

T

A

G

N

N

F

L

I

G

Q

N

L

T

L

L

N

N

P

P

G

D

L

S

V

V

L

I

S

G

G

G

binding magnesium ion: N116 (= N195), N119 (= N198), G148 (≠ T227)
binding zinc ion: H169 (= H248), C179 (= C258), C181 (= C260), C186 (= C265)

3eo3A Crystal structure of the n-acetylmannosamine kinase domain of human gne protein (see paper)
32% identity, 42% coverage: 179:350/406 of query aligns to 71:245/288 of 3eo3A

query
sites
3eo3A

L

L

K

R

A

T

I

P

V

L

E

S

E

D

R

T

T

L

G

H

L

L

P

P

V

V

I

W

I

V

D

D

N

N

G

D

A

G

N

N

G

C

A

A

V

A

L

L

A

A

E

E

T

R

R

K

Y

F

G

G

S

Q

G

G

K

K

G

G

M

L

K

E

S

N

V

F

I

V

Y

T

L

L

N

I

C

T

G

G

V

T

G

G

I

I

R

G

T

G

G

G

V

I

I

I

S

H

S

Q

G

H

T

E

L

L

V

I

R

H

T

G

I

S

N

S

D

F

A

C

D

A

T

E

F

L

A

G

H
|
H

M

L

V

V

I

V

D

S

V

L

N

D

G

G

K

P

P

D

C
|
C

H

S

C
|
C

G

G

N

S

Q

H

G

G

C
|
C

V

I

E

E

R

A

Y

Y

S

A

S

S

I

G

Y

M

A

A

I

L

M

Q

-

R

-

E

-

A

E

K

A

K

L

L

A

H

E

D

E

E

M

D

P

L

Q

L

G

L

K

V

D

E

R

G

R

M

N

S

H

V

K

P

A

K

D

D

R

E

P

A

V

V

S

G

Y

A

L

L

E

H

L

L

C

I

R

Q

E

A

A

A

E

K

E

L

N

G

D

N

T

A

I

K

A

A

R

Q

Q

S

V

I

L

L

Q

R

N

T

A

A

A

G

V

T

R

A

M

L

G

G

T

L

G

G

L

V

A

V

N

N

F

I

I

L

Q

H

L

T

L

M

N

N

P

P

G

S

L

L

V

V

V

I

L

L

S

S

G

G

P

V

L

L

I

A

L

S

H

H

binding zinc ion: H140 (= H248), C150 (= C258), C152 (= C260), C157 (= C265)

3lm9A Crystal structure of fructokinase with adp and fructose bound in the active site (see paper)
22% identity, 57% coverage: 122:354/406 of query aligns to 35:239/294 of 3lm9A

query
sites
3lm9A

P

P

Q

D

A

E

T

T

L

I

N

E

R

K

I

V

L

I

D

Q

W

Y

I

F

G

S

R

Q

V

F

M

S

E

L

K

Q

R

-

G

-

H

-

G

-

Y

-

V

-

I

-

G

A

V

I

G

G

I

I

G

G

T

S

V

F

G
|
G

P

P

L

V

D

D

R

N

Q

D

S

K

G

T

-

S

-

Q

-

T

-

Y

-

G

-

T

I

I

I

T

L

A

N

T

P

P

E

K

N

A

F

-

E

-

A

-

Q

-

G

G

W

W

E

R

N

H

I

Y

P

P

L

F

K

L

A

Q

I

T

V

V

E

K

E

N

R

E

T

M

G

K

L

I

P

P

V

V

I

G

I

F

D

S

N

T

G
x
D

A

V

N

N

G

A

A

A

V

A

L

L

A

G

E

E

T

F

R

L

Y

F

G

G

S

E

G

A

K

K

G

G

M

L

K

D

S

S

V

C

I

L

Y

Y

L

I

N

T

C

I

G
|
G

V
x
T

G

G

I
|
I

R

G

T

A

G

G

V

A

I

I

S

V

S

E

G

G

T

R

L

L

V

L

R

Q

T

G

I

L

N

S

D

H

A

P

D
x
E

D

-

T

-

F

M

A

G

H
|
H

M

I

V

Y

I

I

D

R

V

R

N

H

G

P

K

D

P

D

C

V

H

Y

C

Q

G

G

N

-

Q

-

G

-

C

-

V

-

E

-

R

-

Y

-

S

-

I

-

Y

-

A

-

I

-

M

-

E

-

A

-

L

-

A

-

E

-

E

K

M
x
C

P

P

Q

Y

G
x
H

K

G

D

D

R
x
C

R

F

N
x
E

H

G

K

L

A

A

D

S

R
x
G

P
|
P

V

A

S

I

Y
x
E

L

A

E

R

L

W

C

G

R

K

E

K

A
|
A

E

A

E

D

N

L

D

S

T

D

I

I

A

A

R

-

Q

Q

V

V

L

W

Q

E

N

L

A

E

A

G

V

Y

R

Y

M

I

G

A

T

Q

G

A

L

L

A

A

N

Q

F

Y

I

I

Q

L

L

I

L

L

N

A

P

P

G

K

L

K

V

I

L

L

S

G

G

G

P
x
G

L

V

I

M

L

Q

H

Q

S

K

Q

Q

L

V

F

F

binding adenosine-5'-diphosphate: G130 (= G222), T131 (≠ V223), G182 (≠ R296), P183 (= P297), E186 (≠ Y300), A193 (= A307), G231 (≠ P346)
binding beta-D-fructofuranose: G60 (= G154), D104 (≠ G196), I133 (= I225), E151 (≠ D243), E177 (≠ N291)
binding zinc ion: H154 (= H248), C169 (≠ M283), H172 (≠ G286), C175 (≠ R289)

1xc3A Structure of a putative fructokinase from bacillus subtilis (see paper)
22% identity, 57% coverage: 122:354/406 of query aligns to 35:239/295 of 1xc3A

query
sites
1xc3A

P

P

Q

D

A

E

T

T

L

I

N

E

R

K

I

V

L

I

D

Q

W

Y

I

F

G

S

R

Q

V

F

M

S

E

L

K

Q

R

-

G

-

H

-

G

-

Y

-

V

-

I

-

G

A

V

I

G

G

I

I

G

G

T

S

V

F

G

G

P

P

L

V

D

D

R

N

Q

D

S

K

G

T

-

S

-

Q

-

T

-

Y

-

G

-

T

I

I

I

T

L

A

N

T

P

P

E

K

N

A

F

-

E

-

A

-

Q

-

G

G

W

W

E

R

N

H

I

Y

P

P

L

F

K

L

A

Q

I

T

V

V

E

K

E

N

R

E

T

M

G

K

L

I

P

P

V

V

I

G

I

F

D

S

N

T

G

D

A

V

N

N

G

A

A

A

V

A

L

L

A

G

E

E

T

F

R

L

Y

F

G

G

S

E

G

A

K

K

G

G

M

L

K

D

S

S

V

C

I

L

Y

Y

L

I

N

T

C

I

G

G

V

T

G

G

I

I

R

G

T

A

G

G

V

A

I

I

S

V

S

E

G

G

T

R

L

L

V

L

R

Q

T

G

I

L

N

S

D

H

A

P

D

E

D

-

T

-

F

M

A

G

H
|
H

M

I

V

Y

I

I

D

R

V

R

N

H

G

P

K

D

P

D

C

V

H

Y

C

Q

G

G

N

-

Q

-

G

-

C

-

V

-

E

-

R

-

Y

-

S

-

I

-

Y

-

A

-

I

-

M

-

E

-

A

-

L

-

A

-

E

-

E

K

M
x
C

P

P

Q

Y

G
x
H

K

G

D

D

R
x
C

R

F

N

E

H

G

K

L

A

A

D

S

R

G

P

P

V

A

S

I

Y

E

L

A

E

R

L

W

C

G

R

K

E

K

A

A

E

A

E

D

N

L

D

S

T

D

I

I

A

A

R

-

Q

Q

V

V

L

W

Q

E

N

L

A

E

A

G

V

Y

R

Y

M

I

G

A

T

Q

G

A

L

L

A

A

N

Q

F

Y

I

I

Q

L

L

I

L

L

N

A

P

P

G

K

L

K

V

I

L

L

S

G

G

G

P

G

L

V

I

M

L

Q

H

Q

S

K

Q

Q

L

V

F

F

binding zinc ion: H154 (= H248), C169 (≠ M283), H172 (≠ G286), C175 (≠ R289)

6jdoA Crystal structure of n-acetyl mannosmaine kinase with amp-pnp from pasteurella multocida
24% identity, 57% coverage: 120:349/406 of query aligns to 30:245/293 of 6jdoA

query
sites
6jdoA

S

A

S

T

P

P

Q

Q

A

A

-

D

T

A

L

A

N

N

R

A

I

M

L

H

D

D

W

T

I

L

G

A

R

N

V

I

M

L

E

A

K

L

R

Y

G

A

H

-

G

G

Y

Q

V

F

I

D

G

Y

V

V

G

A

I

V

G

A

T

S

V

T

G

G

P

I

L

I

D

N

R

H

Q

G

S

V

G

L

I

T

I

A

L

L

N

N

P

P

E

K

N

N

F

L

E

-

A

-

Q

G

G

G

W

L

E

A

N

E

I

F

P

P

L

L

K

K

A

E

I

S

V

I

E

A

E

R

R

H

T

T

G

D

L

K

P

P

V

I

I

G

I

L

D

L

N

N

G

D

A

V

N

Q

G

A

A

A

V

A

L

C

A

A

E

E

T

Y

R

K

Y

D

G

E

S

D

G

K

K

N

G

A

M

V

K

Q

S

N

V

F

I

V

Y

F

L

I

N

T

C

V

G
x
S

V
x
T

G

G

I

V

R

G

T

G

G

G

V

I

I

I

S

L

S

E

G

R

T

R

L

L

V

L

R

T

T

E

I

P

N

N

D

G

A

V

D

A

D

G

T

H

F

I

A

G

H
|
H

M

T

V

L

I

A

D

D

V

P

N

N

G

G

K

P

P

V

C
|
C

H

G

C
|
C

G

G

N
x
R

Q

V

G

G

C

C

V

V

E

E

R

A

Y

V

S

A

I
x
G

Y

R

A

A

I

I

M

E

E

A

A

V

L

S

A

S

E

Q

E

W

M

N

P

P

Q

P

G

C

K

T

D

-

R

-

N

-

H

-

K

-

A

-

D

-

R

-

P
|
P

V

K

S

Q

Y

A

L
x
F

E

E

L

L

C

F

R

R

E

-

A

-

E

-

E

K

N

N

D

D

T

E

I

K

A

A

R

T

Q

A

V

L

L

I

Q

Q

N

R

A

S

A

A

V

S

R

A

M

I

G

A

T

N

G

L

L

I

A

A

N

D

F

L

I

V

Q

I

L

G

L

L

N

D

P

V

G

Q

L

K

V

V

L

V

S

G

G

G

P
x
S

L

V

I

G

L

L

binding phosphoaminophosphonic acid-adenylate ester: S130 (≠ G222), T131 (≠ V223), G180 (≠ I272), P196 (= P297), F200 (≠ L301), S242 (≠ P346)
binding calcium ion: H156 (= H248), C166 (= C258), C168 (= C260), R170 (≠ N262)

6jdhA Crystal structure of n-acetyl mannosmaine kinase from pasteurella multocida
24% identity, 57% coverage: 120:349/406 of query aligns to 30:245/293 of 6jdhA

query
sites
6jdhA

S

A

S

T

P

P

Q

Q

A

A

-

D

T

A

L

A

N

N

R

A

I

M

L

H

D

D

W

T

I

L

G

A

R

N

V

I

M

L

E

A

K

L

R

Y

G

A

H

-

G

G

Y

Q

V

F

I

D

G

Y

V

V

G

A

I

V

G

A

T

S

V

T

G

G

P

I

L

I

D

N

R

H

Q

G

S

V

G

L

I

T

I

A

L

L

N

N

P

P

E

K

N

N

F

L

E

-

A

-

Q

G

G

G

W

L

E

A

N

E

I

F

P

P

L

L

K

K

A

E

I

S

V

I

E

A

E

R

R

H

T

T

G

D

L

K

P

P

V

I

I

G

I

L

D

L

N

N

G

D

A

V

N

Q

G

A

A

A

V

A

L

C

A

A

E

E

T

Y

R

K

Y

D

G

E

S

D

G

K

K

N

G

A

M

V

K

Q

S

N

V

F

I

V

Y

F

L

I

N

T

C

V

G

S

V

T

G

G

I

V

R

G

T

G

G

G

V

I

I

I

S

L

S

E

G

R

T

R

L

L

V

L

R

T

T

E

I

P

N

N

D

G

A

V

D

A

D

G

T

H

F

I

A

G

H
|
H

M

T

V

L

I

A

D

D

V

P

N

N

G

G

K

P

P

V

C
|
C

H

G

C
|
C

G

G

N

R

Q

V

G

G

C
|
C

V

V

E

E

R

A

Y

V

S

A

I

G

Y

R

A

A

I

I

M

E

E

A

A

V

L

S

A

S

E

Q

E

W

M

N

P

P

Q

P

G

C

K

T

D

-

R

-

N

-

H

-

K

-

A

-

D

-

R

-

P

P

V

K

S

Q

Y

A

L

F

E

E

L

L

C

F

R

R

E

-

A

-

E

-

E

K

N

N

D

D

T

E

I

K

A

A

R

T

Q

A

V

L

L

I

Q

Q

N

R

A

S

A

A

V

S

R

A

M

I

G

A

T

N

G

L

L

I

A

A

N

D

F

L

I

V

Q

I

L

G

L

L

N

D

P

V

G

Q

L

K

V

V

L

V

S

G

G

G

P

S

L

V

I

G

L

L

binding zinc ion: H156 (= H248), C166 (= C258), C168 (= C260), C173 (= C265)

7p9lAAA Ubiquitin-like protein SMT3,N-acetyl-D-glucosamine kinase
25% identity, 67% coverage: 109:382/406 of query aligns to 13:300/303 of 7p9lAAA

query
sites
7p9lAAA

I

I

E

E

K

F

D

G

R

A

F

F

D

D

M

A

D

D

-

L

-

V

-

R

-

V

-

A

-

R

-

E

R

R

N

V

S

A

S

T

P

P

Q

T

A

E

T

S

L

Y

N

A

R

A

I

F

L

L

D

D

W

A

I

I

G

V

R

T

V

L

M

V

E

N

K

N

R

A

G

D

H

A

G

E

Y

F

V

G

I

V

G

K

-

G

-

T

V

V

G

G

I

I

G

G

T

I

V
x
P

G
|
G

P

I

L

A

D

D

R

V

Q

E

S

T

G

G

I

K

I

L

L

L

N

T

P
x
S

E

N

N

I

F

P

E

A

A

A

Q

M

G

G

W

H

E

T

N

-

I

-

P

-

L

L

K

Q

A

R

I

D

V

L

E

E

R

R

T

L

G

Q

L

R

P

P

V

V

I

K

I

I

D

E

N
|
N

G
x
D

A

A

N

N

G

C

A

F

V

A

L

L

A

S

E

E

T

A

R

W

Y

D

G

E

S

D

G

L

K

R

G

G

M

E

K

P

S

S

V

V

I

L

Y

G

L

L

N

I

C

L

G
|
G

V
x
T

G
|
G

I
x
V

R
x
G

T

G

G

G

V

L

I

I

S

F

S

N

G

G

T

K

L

V

V

H

R

S

T

G

I

R

N

A

D

N

A

I

D

A

D

G

T
x
E

F

I

A

G

H
|
H

M

T

V

R

I

L

D

P

V

Y

N

D

G

A

-

L

-

K

-

L

-

G

-

M

-

E

-

N

-

A

-

P

-

I

K

F

P

P

C
|
C

H

G

C
|
C

G

K

N

N

Q

S

G

G

C
|
C

V

I

E
x
D

R

N

Y

Y

S

L

S

S

I

G

Y

R

A

G

I

F

M

-

E

-

A

-

L

-

A

-

E

-

M

-

P

-

Q

-

G

-

K

E

D

Q

R

L

R

Y

N

D

H

H

K

Y

A

F

D

S

R

E

P

K

V

L

S

S

Y

A

L

P

E
|
E

L

I

C

I

R

A

E
x
H

A

Y

E

E

E

Q

N

G

D

E

T

R

I

R

A

A

R

V

Q

Q

V

H

L

V

Q

E

N

R

A

F

A

M

V

E

R

L

M

L

G

A

T

I

G

C

L

L

A

A

N

N

F

I

I

F

Q

T

L

C

L

L

N

D

P

P

G

H

L

V

V

V

L

L

S

G

G

G

-

G

-

L

-

S

-

N

-

F

-

E

-

L

-

I

-

Y

-

Q

-

E

-

L

P

P

L

K

I

R

L

L

H

P

S

A

Q

H

L

L

F

L

Y

H

E

V

V

A

C

K

V

L

E

P

A

K

A

I

K

I

R

K

R

A

R

R

P

H

W

G

D

D

K

A

G

G

H

-

L

-

V

-

F

-

S

V

R

R

G

G

G

A

A

A

F

F

binding 2-acetamido-2-deoxy-6-O-phosphono-beta-D-glucopyranose: P66 (≠ V153), G67 (= G154), S79 (≠ P166), N105 (= N195), D106 (≠ G196), G132 (= G222), T133 (≠ V223), G134 (= G224), V135 (≠ I225), G136 (≠ R226), E155 (≠ T245), H158 (= H248), D188 (≠ E267)
binding zinc ion: H158 (= H248), C179 (= C258), C181 (= C260), C186 (= C265), E212 (= E302), H216 (≠ E306)

Query Sequence

>WP_015945030.1 NCBI__GCF_000021925.1:WP_015945030.1
MRTGVLIYKDVLSSLSPEAKKILGLILHKGGMTKSQLAKVAGLKLTTLNRMMLPLEKADL
ILPTETGESTGGRKPVIYDVNPRRYYVIGIDISRLYSQVVLTNLKMELIEKDRFDMDRNS
SPQATLNRILDWIGRVMEKRGHGYVIGVGIGTVGPLDRQSGIILNPENFEAQGWENIPLK
AIVEERTGLPVIIDNGANGAVLAETRYGSGKGMKSVIYLNCGVGIRTGVISSGTLVRTIN
DADDTFAHMVIDVNGKPCHCGNQGCVERYSSIYAIMEALAEEMPQGKDRRNHKADRPVSY
LELCREAEENDTIARQVLQNAAVRMGTGLANFIQLLNPGLVVLSGPLILHSQLFYEVCVE
AAKRRRPWDKGGHLVFSRGGAFEENAISIGAAALVVEHYLEPETLG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory