SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_016855770.1 NCBI__GCF_000265245.1:WP_016855770.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9YHT2 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; EC 1.3.5.1 from Gallus gallus (Chicken) (see 2 papers)
32% identity, 24% coverage: 7:107/413 of query aligns to 178:278/290 of Q9YHT2

query
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Q9YHT2

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C196 (= C25) binding [4Fe-4S] cluster
C199 (= C28) binding [4Fe-4S] cluster
C202 (= C31) binding [4Fe-4S] cluster
C206 (= C35) binding [3Fe-4S] cluster
W211 (≠ L40) binding a ubiquinone
C253 (= C78) binding [3Fe-4S] cluster
C259 (= C84) binding [3Fe-4S] cluster
C263 (= C88) binding [4Fe-4S] cluster

Sites not aligning to the query:

103 binding [2Fe-2S] cluster
108 binding [2Fe-2S] cluster
111 binding [2Fe-2S] cluster
123 binding [2Fe-2S] cluster

Q007T0 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; EC 1.3.5.1 from Sus scrofa (Pig) (see paper)
30% identity, 24% coverage: 7:107/413 of query aligns to 168:268/280 of Q007T0

query
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Q007T0

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C186 (= C25) binding [4Fe-4S] cluster
C189 (= C28) binding [4Fe-4S] cluster
C192 (= C31) binding [4Fe-4S] cluster
C196 (= C35) binding [3Fe-4S] cluster
W201 (≠ L40) binding a ubiquinone
C243 (= C78) binding [3Fe-4S] cluster
C249 (= C84) binding [3Fe-4S] cluster
C253 (= C88) binding [4Fe-4S] cluster

Sites not aligning to the query:

93 binding [2Fe-2S] cluster
98 binding [2Fe-2S] cluster
101 binding [2Fe-2S] cluster
113 binding [2Fe-2S] cluster

1yq3B Avian respiratory complex ii with oxaloacetate and ubiquinone (see paper)
32% identity, 24% coverage: 7:107/413 of query aligns to 135:235/242 of 1yq3B

query
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1yq3B

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binding fe3-s4 cluster: C163 (= C35), Y173 (≠ R45), P176 (= P48), C210 (= C78), T212 (= T80), I213 (≠ C81), M214 (≠ R82), N215 (= N83), C216 (= C84)
binding iron/sulfur cluster: C153 (= C25), I154 (≠ V26), L155 (≠ H27), C156 (= C28), A157 (≠ G29), C159 (= C31), A177 (≠ R49), C220 (= C88), P221 (= P89)
binding Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer: P164 (= P36), W168 (≠ L40), I213 (≠ C81)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 60, 61, 63, 65, 66, 67, 68, 80

8gs8B Cryo-em structure of the human respiratory complex ii (see paper)
32% identity, 21% coverage: 7:93/413 of query aligns to 134:224/239 of 8gs8B

query
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8gs8B

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binding fe3-s4 cluster: C162 (= C35), Y172 (≠ R45), P175 (= P48), C209 (= C78), H210 (≠ L79), T211 (= T80), I212 (≠ C81), M213 (≠ R82), N214 (= N83), C215 (= C84)
binding iron/sulfur cluster: C152 (= C25), C155 (= C28), C158 (= C31), A176 (≠ R49), C219 (= C88)
binding ubiquinone-1: P163 (= P36), W167 (≠ L40), I212 (≠ C81)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 59, 60, 64, 65, 66, 67, 79

6myqB Avian mitochondrial complex ii with ferulenol bound (see paper)
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 6myqB

query
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6myqB

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binding 4-oxidanyl-3-[(2~{E},6~{E})-3,7,11-trimethyldodeca-2,6,10-trienyl]chromen-2-one: W165 (≠ L40), I210 (≠ C81)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C78), T209 (= T80), I210 (≠ C81), M211 (≠ R82), N212 (= N83), C213 (= C84)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C88), P218 (= P89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3sfeB Crystal structure of porcine mitochondrial respiratory complex ii bound with oxaloacetate and thiabendazole (see paper)
32% identity, 24% coverage: 7:107/413 of query aligns to 133:233/240 of 3sfeB

query
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3sfeB

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binding fe3-s4 cluster: C161 (= C35), Y171 (≠ R45), P174 (= P48), C208 (= C78), T210 (= T80), I211 (≠ C81), M212 (≠ R82), N213 (= N83), C214 (= C84)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), A155 (≠ G29), C157 (= C31), A175 (≠ R49), C218 (= C88), P219 (= P89), K220 (≠ S90), L222 (≠ V92)
binding 2-(1,3-thiazol-4-yl)-1h-benzimidazole: H209 (≠ L79), I211 (≠ C81)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 58, 59, 61, 63, 64, 65, 66, 78

3sfdB Crystal structure of porcine mitochondrial respiratory complex ii bound with oxaloacetate and pentachlorophenol (see paper)
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3sfdB

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binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C78), T209 (= T80), I210 (≠ C81), M211 (≠ R82), N212 (= N83), C213 (= C84)
binding pentachlorophenol: P161 (= P36), W165 (≠ L40), I210 (≠ C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C88), P218 (= P89), K219 (≠ S90)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 64, 65, 77

3aegB Crystal structure of porcine heart mitochondrial complex ii bound with n-biphenyl-3-yl-2-iodo-benzamide
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3aegB

query
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3aegB

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binding N-biphenyl-3-yl-2-iodobenzamide: S162 (≠ T37), W164 (≠ Q39), W165 (≠ L40), H208 (≠ L79)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C78), I210 (≠ C81), M211 (≠ R82), C213 (= C84), I227 (≠ L98)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C88)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 75, 77

3aeeB Crystal structure of porcine heart mitochondrial complex ii bound with atpenin a5
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3aeeB

query
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3aeeB

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P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

L

I

E

D

N

S

P

R

E

D

D

D

D

-

V

-

V

F

T

T

E

E

T

R

R

L

-

A

-

K

-

L

-

Q

-

D

-

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

E

N

Y

P

H

G

K

K

L

A

L

I

D

-

I

-

G

-

R

-

A

A

E

E

I

I

E

K

R

K

binding 3-[(2s,4s,5r)-5,6-dichloro-2,4-dimethyl-1-oxohexyl]-4-hydroxy-5,6-dimethoxy-2(1h)-pyridinone: W165 (≠ L40), H208 (≠ L79), I210 (≠ C81)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C78), T209 (= T80), I210 (≠ C81), M211 (≠ R82), N212 (= N83), C213 (= C84)
binding iron/sulfur cluster: C150 (= C25), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C88), P218 (= P89), L221 (≠ V92)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3aedB Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-phenyl-benzamide
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3aedB

query
sites
3aedB

E

Q

Q

Q

D

Y

L

L

E

Q

Q

S

P

I

H

E

I

E

R

R

E

E

A

K

E

L

R

D

V

G

L

L

R

Y

T

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

D

L

G

G

P
|
P

R

A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

L

I

E

D

N

S

P

R

E

D

D

D

D

-

V

-

V

F

T

T

E

E

T

R

R

L

-

A

-

K

-

L

-

Q

-

D

-

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P

P

S

K

G

G

V
x
L

E

N

Y

P

H

G

K

K

L

A

L

I

D

-

I

-

G

-

R

-

A

A

E

E

I

I

E

K

R

K

binding 2-iodo-N-phenylbenzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L79), I210 (≠ C81)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C78), I210 (≠ C81), M211 (≠ R82), N212 (= N83), C213 (= C84)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), C217 (= C88), L221 (≠ V92)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3aecB Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-(1-methylethyl)-benzamid
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3aecB

query
sites
3aecB

E

Q

Q

Q

D

Y

L

L

E

Q

Q

S

P

I

H

E

I

E

R

R

E

E

A

K

E

L

R

D

V

G

L

L

R

Y

T

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

L

I

E

D

N

S

P

R

E

D

D

D

D

-

V

-

V

F

T

T

E

E

T

R

R

L

-

A

-

K

-

L

-

Q

-

D

-

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

E

N

Y

P

H

G

K

K

L

A

L

I

D

-

I

-

G

-

R

-

A

A

E

E

I

I

E

K

R

K

binding 2-iodo-N-(1-methylethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L79)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C78), I210 (≠ C81), M211 (≠ R82), N212 (= N83), C213 (= C84)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C88), P218 (= P89), L221 (≠ V92)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 62, 63, 65, 75, 77

3aebB Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-phenoxy-phenyl)-2-trifluoromethyl-benzamide
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3aebB

query
sites
3aebB

E

Q

Q

Q

D

Y

L

L

E

Q

Q

S

P

I

H

E

I

E

R

R

E

E

A

K

E

L

R

D

V

G

L

L

R

Y

T

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

L

I

E

D

N

S

P

R

E

D

D

D

D

-

V

-

V

F

T

T

E

E

T

R

R

L

-

A

-

K

-

L

-

Q

-

D

-

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P

P

S

K

G

G

V
x
L

E

N

Y

P

H

G

K

K

L

A

L

I

D

-

I

-

G

-

R

-

A

A

E

E

I

I

E

K

R

K

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C78), T209 (= T80), I210 (≠ C81), M211 (≠ R82), N212 (= N83), C213 (= C84)
binding N-(3-phenoxyphenyl)-2-(trifluoromethyl)benzamide: W165 (≠ L40), H208 (≠ L79)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C88), L221 (≠ V92)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 62, 63, 65, 77

3aeaB Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-dimethylaminomethyl-phenyl)-2-trifluoromethyl-benzamide (see paper)
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3aeaB

query
sites
3aeaB

E

Q

Q

Q

D

Y

L

L

E

Q

Q

S

P

I

H

E

I

E

R

R

E

E

A

K

E

L

R

D

V

G

L

L

R

Y

T

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

L

I

E

D

N

S

P

R

E

D

D

D

D

-

V

-

V

F

T

T

E

E

T

R

R

L

-

A

-

K

-

L

-

Q

-

D

-

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

E

N

Y

P

H

G

K

K

L

A

L

I

D

-

I

-

G

-

R

-

A

A

E

E

I

I

E

K

R

K

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C78), T209 (= T80), I210 (≠ C81), M211 (≠ R82), N212 (= N83), C213 (= C84)
binding N-{3-[(dimethylamino)methyl]phenyl}-2-(trifluoromethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L79), I210 (≠ C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C88), P218 (= P89), L221 (≠ V92)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3ae9B Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-pentafluorophenyloxy-phenyl)-2-trifluoromethyl-benzamide (see paper)
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3ae9B

query
sites
3ae9B

E

Q

Q

Q

D

Y

L

L

E

Q

Q

S

P

I

H

E

I

E

R

R

E

E

A

K

E

L

R

D

V

G

L

L

R

Y

T

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

L

I

E

D

N

S

P

R

E

D

D

D

D

-

V

-

V

F

T

T

E

E

T

R

R

L

-

A

-

K

-

L

-

Q

-

D

-

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P

P

S

K

G

G

V
x
L

E

N

Y

P

H

G

K

K

L

A

L

I

D

-

I

-

G

-

R

-

A

A

E

E

I

I

E

K

R

K

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C78), H208 (≠ L79), T209 (= T80), I210 (≠ C81), M211 (≠ R82), N212 (= N83), C213 (= C84)
binding N-[3-(pentafluorophenoxy)phenyl]-2-(trifluoromethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L79)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C88), L221 (≠ V92)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3ae8B Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-isopropoxy-phenyl)-2-trifluoromethylbenzamide
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3ae8B

query
sites
3ae8B

E

Q

Q

Q

D

Y

L

L

E

Q

Q

S

P

I

H

E

I

E

R

R

E

E

A

K

E

L

R

D

V

G

L

L

R

Y

T

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P

P

T
x
S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

R

Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

L

I

E

D

N

S

P

R

E

D

D

D

D

-

V

-

V

F

T

T

E

E

T

R

R

L

-

A

-

K

-

L

-

Q

-

D

-

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N

N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

E

N

Y

P

H

G

K

K

L

A

L

I

D

-

I

-

G

-

R

-

A

A

E

E

I

I

E

K

R

K

binding fe3-s4 cluster: C160 (= C35), P173 (= P48), C207 (= C78), T209 (= T80), I210 (≠ C81), M211 (≠ R82), C213 (= C84)
binding N-[3-(1-methylethoxy)phenyl]-2-(trifluoromethyl)benzamide: S162 (≠ T37), W165 (≠ L40), H208 (≠ L79)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C88), P218 (= P89), L221 (≠ V92)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 62, 63, 65, 75, 77

3ae7B Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-(3-isopropoxy-phenyl)-benzamide (see paper)
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3ae7B

query
sites
3ae7B

E

Q

Q

Q

D

Y

L

L

E

Q

Q

S

P

I

H

E

I

E

R

R

E

E

A

K

E

L

R

D

V

G

L

L

R

Y

T

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

L

I

E

D

N

S

P

R

E

D

D

D

D

-

V

-

V

F

T

T

E

E

T

R

R

L

-

A

-

K

-

L

-

Q

-

D

-

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N

N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V

L

E

N

Y

P

H

G

K

K

L

A

L

I

D

-

I

-

G

-

R

-

A

A

E

E

I

I

E

K

R

K

binding 2-iodo-N-[3-(1-methylethoxy)phenyl]benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L79), I210 (≠ C81)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C78), T209 (= T80), I210 (≠ C81), M211 (≠ R82), C213 (= C84)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C88), P218 (= P89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 75, 77

3ae6B Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-isopropoxy-phenyl)-phthalamicacid
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3ae6B

query
sites
3ae6B

E

Q

Q

Q

D

Y

L

L

E

Q

Q

S

P

I

H

E

I

E

R

R

E

E

A

K

E

L

R

D

V

G

L

L

R

Y

T

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P

P

T
x
S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

L

I

E

D

N

S

P

R

E

D

D

D

D

-

V

-

V

F

T

T

E

E

T

R

R

L

-

A

-

K

-

L

-

Q

-

D

-

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L

H

T

T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

E

N

Y

P

H

G

K

K

L

A

L

I

D

-

I

-

G

-

R

-

A

A

E

E

I

I

E

K

R

K

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C78), I210 (≠ C81), M211 (≠ R82), N212 (= N83), C213 (= C84)
binding 2-{[3-(1-methylethoxy)phenyl]carbamoyl}benzoic acid: S162 (≠ T37), W165 (≠ L40)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C88), P218 (= P89), L221 (≠ V92)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 56, 57, 62, 63, 65, 77

3ae4B Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-methyl-benzamide
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3ae4B

query
sites
3ae4B

E

Q

Q

Q

D

Y

L

L

E

Q

Q

S

P

I

H

E

I

E

R

R

E

E

A

K

E

L

R

D

V

G

L

L

R

Y

T

E

C
|
C

V
x
I

H

L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P

P

T
x
S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

R

Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

L

I

E

D

N

S

P

R

E

D

D

D

D

-

V

-

V

F

T

T

E

E

T

R

R

L

-

A

-

K

-

L

-

Q

-

D

-

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V

L

E

N

Y

P

H

G

K

K

L

A

L

I

D

-

I

-

G

-

R

-

A

A

E

E

I

I

E

K

R

K

binding fe3-s4 cluster: C160 (= C35), S162 (≠ T37), P173 (= P48), C207 (= C78), M211 (≠ R82), N212 (= N83), C213 (= C84)
binding 2-iodo-N-methylbenzamide: W165 (≠ L40), H208 (≠ L79), I210 (≠ C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C88), P218 (= P89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 75, 77

3ae3B Crystal structure of porcine heart mitochondrial complex ii bound with 2-nitro-n-phenyl-benzamide
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3ae3B

query
sites
3ae3B

E

Q

Q

Q

D

Y

L

L

E

Q

Q

S

P

I

H

E

I

E

R

R

E

E

A

K

E

L

R

D

V

G

L

L

R

Y

T

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

R
x
Y

D

L

G

G

P
|
P

R

A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

L

I

E

D

N

S

P

R

E

D

D

D

D

-

V

-

V

F

T

T

E

E

T

R

R

L

-

A

-

K

-

L

-

Q

-

D

-

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

E

N

Y

P

H

G

K

K

L

A

L

I

D

-

I

-

G

-

R

-

A

A

E

E

I

I

E

K

R

K

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C78), T209 (= T80), I210 (≠ C81), M211 (≠ R82), N212 (= N83), C213 (= C84)
binding 2-nitro-N-phenylbenzamide: P161 (= P36), S162 (≠ T37), W165 (≠ L40), H208 (≠ L79)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), C217 (= C88), P218 (= P89), L221 (≠ V92)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3ae1B Crystal structure of porcine heart mitochondrial complex ii bound with n-phenyl-2-(trifluoromethyl)-benzamide
32% identity, 24% coverage: 7:107/413 of query aligns to 132:232/239 of 3ae1B

query
sites
3ae1B

E

Q

Q

Q

D

Y

L

L

E

Q

Q

S

P

I

H

E

I

E

R

R

E

E

A

K

E

L

R

D

V

G

L

L

R

Y

T

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

L

W

L

N

G

G

D

D

E

K

R
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

L

I

E

D

N

S

P

R

E

D

D

D

D

-

V

-

V

F

T

T

E

E

T

R

R

L

-

A

-

K

-

L

-

Q

-

D

-

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P

P

S
x
K

G

G

V
x
L

E

N

Y

P

H

G

K

K

L

A

L

I

D

-

I

-

G

-

R

-

A

A

E

E

I

I

E

K

R

K

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ R45), P173 (= P48), C207 (= C78), T209 (= T80), I210 (≠ C81), M211 (≠ R82), N212 (= N83), C213 (= C84)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C88), K219 (≠ S90), L221 (≠ V92)
binding N-phenyl-2-(trifluoromethyl)benzamide: H208 (≠ L79), I210 (≠ C81)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

Query Sequence

>WP_016855770.1 NCBI__GCF_000265245.1:WP_016855770.1
MQTHFTEQDLEQPHIREAERVLRTCVHCGFCNATCPTYQLLGDERDGPRGRIYLMKQMLE
NPEDDVVTEETRLHLDRCLTCRNCETTCPSGVEYHKLLDIGRAEIERRAPRPAAERALRF
GLRKALVEPARFQALLKLGQTFRPLVPGTLRDKMPPAPVDAGSRPEGRRHERQMLILEGC
VQPGLSPNTNAATARVLDRLGIGLTPAPEAGCCGAIDYHLNAQDAGRARMRANIDAWWPH
IDKEGEAGVEAIVQTASGCGAMVKEYGEVLADDPDYADKAARISALAKDLVEILRDEDLE
PLTLREHKRLAFHCPCTLQHAQKLGGSVDTVLARLGFSLTPVQDAHLCCGSAGTYSVTQP
ELATELRDNKLDALEAGDPEVIVTANIGCQTHLNGAGRTPVRHWIEIVDEALA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory