SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_016986598.1 NCBI__GCF_003031005.1:WP_016986598.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1uskA L-leucine-binding protein with leucine bound (see paper)
59% identity, 90% coverage: 28:364/375 of query aligns to 1:337/345 of 1uskA

query
sites
1uskA

D

D

T

D

I

I

K

K

I

V

G

A

I

V

A

V

G

G

P

A

K

M

T

S

G

G

P

P

V

I

A

A

Q

Q

Y

W

G

G

D

D

M

M

Q

E

F

F

S

N

G

G

A

A

K

R

M

Q

A

A

I

I

E

K

Q

D

I

I

N

N

A

A

K

K

G

G

G

G

V

I

D

K

G

G

K

D

K

K

L

L

E

V

A

G

V

V

E

E

Y

Y

D

D

D

D

A

A

C

C

D

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

V

I

V

V

N

N

D

D

G

G

V

I

K

K

F

Y

V

V

V

I

G

G

H

H

L

L

C
|
C

S
|
S

S

S

S

S

T

T

Q

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

D

D

E

E

G

G

V

I

I

L

M

M

I

I

T

S

P

P

A
x
G

A

A

T
|
T

S

N

P

P

D

E

I

L

T

T

A

Q

R

R

G

G

Y

Y

K

Q

M

H

I

I

F

M

R

R

T

T

I

A

G

G

L

L

D

D

S

S

A

S

Q

Q

G

G

P

P

A

T

A

A

G

A

N

K

Y

Y

I

I

A

L

D

E

H

T

V

V

K

K

P

P

K

Q

I

R

V

I

G

A

V

I

L

I

H

H

D

D

K

K

Q

Q

Q

Q

Y
|
Y

G

G

E

E

G

G

I

L

A

A

T

R

A

S

V

V

K

Q

S

D

T

G

L

L

E

K

K

A

K

A

G

N

T

A

K

N

V

V

A

V

V

F

F

F

E

D

G

G

V

I

N

T

A

A

G

G

D

E

K

K

D

D

F

F

S

S

A

A

I

L

I

I

A

A

K

R

L

L

K

K

Q

K

A

E

N

N

V

I

D

D

F

F

V

V

Y

Y

Y

Y

G

G

G

G

Y
|
Y

H

Y

P

P

E

E

L

M

G

G

L

Q

I

M

L

L

R

R

Q

Q

A

A

Q

R

E

S

K

V

G

G

L

L

K

K

A

T

K

Q

F

F

M

M

G

G

P

P

E
|
E

G

G

V

V

G

G

N

N

D

A

S

S

I

L

T

S

Q

N

I

I

A

A

K

G

D

D

A

A

A

A

E

E

G

G

L

M

L

L

V

V

T

T

L

M

P

P

K

K

S

R

F

Y

D

D

Q

Q

D

D

P

P

A

A

N

N

I

Q

A

G

L

I

A

V

D

D

A

A

F

L

K

K

A

A

K

D

K

K

E

K

D

D

P

P

S

S

G

G

P

P

F
x
Y

V

V

F

W

P

I

S

T

Y

Y

S

A

A

A

V

V

E

Q

V

S

I

L

A

A

E

T

G

A

I

L

K

E

A

R

A

T

K

G

S

S

E

D

D

E

A

P

T

L

K

A

V

L

A

V

E

K

A

D

I

L

H

K

A

A

G

N

T

G

F

A

K

N

T

T

P

V

T

I

G

G

D

P

L

L

S

N

F

W

D

D

A

E

K

K

G

G

D

D

L

L

K

K

D

G

F

F

K

D

F

F

V

G

V

V

Y

F

E

Q

W

W

H

H

binding leucine: C78 (= C105), S79 (= S106), G100 (≠ A127), T102 (= T129), Y150 (= Y177), Y202 (= Y229), E226 (= E253), Y276 (≠ F303)

1usiA L-leucine-binding protein with phenylalanine bound (see paper)
59% identity, 90% coverage: 28:364/375 of query aligns to 1:337/345 of 1usiA

query
sites
1usiA

D

D

T

D

I

I

K

K

I

V

G

A

I

V

A

V

G

G

P

A

K

M

T

S

G

G

P

P

V

I

A

A

Q

Q

Y
x
W

G

G

D

D

M

M

Q

E

F

F

S

N

G

G

A

A

K

R

M

Q

A

A

I

I

E

K

Q

D

I

I

N

N

A

A

K

K

G

G

G

G

V

I

D

K

G

G

K

D

K

K

L

L

E

V

A

G

V

V

E

E

Y

Y

D

D

D

D

A

A

C

C

D

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

V

I

V

V

N

N

D

D

G

G

V

I

K

K

F

Y

V

V

V

I

G

G

H

H

L
|
L

C
|
C

S
|
S

S

S

S

S

T

T

Q

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

D

D

E

E

G

G

V

I

I

L

M

M

I

I

T

S

P

P

A
x
G

A

A

T
|
T

S

N

P

P

D

E

I

L

T

T

A

Q

R

R

G

G

Y

Y

K

Q

M

H

I

I

F

M

R

R

T

T

I

A

G

G

L

L

D

D

S

S

A

S

Q

Q

G

G

P

P

A

T

A

A

G

A

N

K

Y

Y

I

I

A

L

D

E

H

T

V

V

K

K

P

P

K

Q

I

R

V

I

G

A

V

I

L

I

H

H

D

D

K

K

Q

Q

Q

Q

Y
|
Y

G

G

E

E

G

G

I

L

A

A

T

R

A

S

V

V

K

Q

S

D

T

G

L

L

E

K

K

A

K

A

G

N

T

A

K

N

V

V

A

V

V

F

F

F

E

D

G

G

V

I

N

T

A

A

G

G

D

E

K

K

D

D

F

F

S

S

A

A

I

L

I

I

A

A

K

R

L

L

K

K

Q

K

A

E

N

N

V

I

D

D

F

F

V

V

Y

Y

Y

Y

G

G

G

G

Y

Y

H

Y

P

P

E

E

L

M

G

G

L

Q

I

M

L

L

R

R

Q

Q

A

A

Q

R

E

S

K

V

G

G

L

L

K

K

A

T

K

Q

F

F

M

M

G

G

P

P

E
|
E

G
|
G

V

V

G

G

N

N

D

A

S

S

I

L

T

S

Q

N

I

I

A

A

K

G

D

D

A

A

A

A

E

E

G

G

L

M

L

L

V

V

T

T

L

M

P

P

K

K

S

R

F

Y

D

D

Q

Q

D

D

P

P

A

A

N

N

I

Q

A

G

L

I

A

V

D

D

A

A

F

L

K

K

A

A

K

D

K

K

E

K

D

D

P

P

S

S

G

G

P

P

F
x
Y

V

V

F

W

P

I

S

T

Y

Y

S

A

A

A

V

V

E

Q

V

S

I

L

A

A

E

T

G

A

I

L

K

E

A

R

A

T

K

G

S

S

E

D

D

E

A

P

T

L

K

A

V

L

A

V

E

K

A

D

I

L

H

K

A

A

G

N

T

G

F

A

K

N

T

T

P

V

T

I

G

G

D

P

L

L

S

N

F

W

D

D

A

E

K

K

G

G

D

D

L

L

K

K

D

G

F

F

K

D

F

F

V

G

V

V

Y

F

E

Q

W

W

H

H

binding phenylalanine: W18 (≠ Y45), L77 (= L104), C78 (= C105), S79 (= S106), G100 (≠ A127), T102 (= T129), Y150 (= Y177), E226 (= E253), G227 (= G254), Y276 (≠ F303)

1z18A Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound valine (see paper)
60% identity, 89% coverage: 30:364/375 of query aligns to 3:335/344 of 1z18A

query
sites
1z18A

I

I

K

K

I

V

G

A

I

V

A

V

G

G

P

A

K

M

T

S

G

G

P

P

V

V

A

A

Q

Q

Y

Y

G

G

D

D

M

Q

Q

E

F

F

S

T

G

G

A

A

K

E

M

Q

A

A

I

V

E

A

Q

D

I

I

N

N

A

A

K

K

G

G

G

G

V

I

D

K

G

G

K

N

K

K

L

L

E

Q

A

I

V

V

E

K

Y

Y

D

D

D

D

A

A

C

C

D

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

V

V

V

V

N

N

D

D

G

G

V

I

K

K

F

Y

V

V

V

I

G

G

H

H

L
|
L

C
|
C

S
|
S

S

S

S

S

T

T

Q

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

D

D

E

E

G

G

V

I

I

L

M

M

I

I

T

T

P

P

A
|
A

A

A

T
|
T

S

A

P

P

D

E

I

L

T

T

A

A

R

R

G

G

Y

Y

K

Q

M

L

I

I

F

L

R

R

T

T

I

T

G

G

L

L

D

D

S

S

A

D

Q

Q

G

G

P

P

A

T

A

A

G

A

N

K

Y

Y

I

I

A

L

D

E

H

K

V

V

K

K

P

P

K

Q

I

R

V

I

G

A

V

I

L

V

H

H

D

D

K

K

Q

Q

Q

Q

Y
|
Y

G

G

E

E

G

G

I

L

A

A

T

R

A

A

V

V

K

Q

S

D

T

G

L

L

E

K

K

K

K

G

G

N

T

A

K

N

V

V

A

V

V

F

F

F

E

D

G

G

V

I

N

T

A

A

G

G

D

E

K

K

D

D

F

F

S

S

A

T

I

L

I

V

A

A

K

R

L

L

K

K

Q

K

A

E

N

N

V

I

D

D

F

F

V

V

Y

Y

Y

Y

G

G

G

G

Y
|
Y

H

H

P

P

E

E

L

M

G

G

L

Q

I

I

L

L

R

R

Q

Q

A

A

Q

R

E

A

K

A

G

G

L

L

K

K

A

T

K

Q

F

F

M

M

G

G

P

P

E
|
E

G

G

V

V

G

A

N

N

D

V

S

S

I

L

T

S

Q

N

I

I

A

A

K

G

D

E

A

S

A

A

E

E

G

G

L

L

L

L

V

V

T

T

L

K

P

P

K

K

S

N

F

Y

D

D

Q

Q

D

V

P

P

A

A

N

N

I

K

A

P

L

I

A

V

D

D

A

A

F

I

K

K

A

A

K

K

K

K

E

Q

D

D

P

P

S

S

G

G

P

A

F

F

V

V

F

W

P

T

S

T

Y

Y

S

A

A

A

V

L

E

Q

V

S

I

L

A

Q

E

A

G

G

I

L

K

N

A

-

A

-

K

Q

S

S

E

D

D

D

A

P

T

A

K

E

V

I

A

A

E

K

A

Y

I

L

H

K

A

A

G

N

T

S

F

V

K

D

T

T

P

V

T

M

G

G

D

P

L

L

S

T

F

W

D

D

A

E

K

K

G

G

D

D

L

L

K

K

D

G

F

F

K

E

F

F

V

G

V

V

Y

F

E

D

W

W

H

H

binding valine: L77 (= L104), C78 (= C105), S79 (= S106), A100 (= A127), T102 (= T129), Y150 (= Y177), Y202 (= Y229), E226 (= E253)

1z17A Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound ligand isoleucine (see paper)
60% identity, 89% coverage: 30:364/375 of query aligns to 3:335/344 of 1z17A

query
sites
1z17A

I

I

K

K

I

V

G

A

I

V

A

V

G

G

P

A

K

M

T

S

G

G

P

P

V

V

A

A

Q

Q

Y
|
Y

G

G

D

D

M

Q

Q

E

F

F

S

T

G

G

A

A

K

E

M

Q

A

A

I

V

E

A

Q

D

I

I

N

N

A

A

K

K

G

G

G

G

V

I

D

K

G

G

K

N

K

K

L

L

E

Q

A

I

V

V

E

K

Y

Y

D

D

D

D

A

A

C

C

D

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

V

V

V

V

N

N

D

D

G

G

V

I

K

K

F

Y

V

V

V

I

G

G

H

H

L
|
L

C
|
C

S
|
S

S

S

S

S

T

T

Q

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

D

D

E

E

G

G

V

I

I

L

M

M

I

I

T

T

P

P

A
|
A

A
|
A

T
|
T

S

A

P

P

D

E

I

L

T

T

A

A

R

R

G

G

Y

Y

K

Q

M

L

I

I

F

L

R

R

T

T

I

T

G

G

L

L

D

D

S

S

A

D

Q

Q

G

G

P

P

A

T

A

A

G

A

N

K

Y

Y

I

I

A

L

D

E

H

K

V

V

K

K

P

P

K

Q

I

R

V

I

G

A

V

I

L

V

H

H

D

D

K

K

Q

Q

Q

Q

Y
|
Y

G

G

E

E

G

G

I

L

A

A

T

R

A

A

V

V

K

Q

S

D

T

G

L

L

E

K

K

K

K

G

G

N

T

A

K

N

V

V

A

V

V

F

F

F

E

D

G

G

V

I

N

T

A

A

G

G

D

E

K

K

D

D

F

F

S

S

A

T

I

L

I

V

A

A

K

R

L

L

K

K

Q

K

A

E

N

N

V

I

D

D

F

F

V

V

Y

Y

Y

Y

G

G

G

G

Y
|
Y

H

H

P

P

E

E

L

M

G

G

L

Q

I

I

L

L

R

R

Q

Q

A

A

Q

R

E

A

K

A

G

G

L

L

K

K

A

T

K

Q

F

F

M

M

G

G

P

P

E
|
E

G

G

V

V

G

A

N

N

D

V

S

S

I

L

T

S

Q

N

I

I

A

A

K

G

D

E

A

S

A

A

E

E

G

G

L

L

L

L

V

V

T

T

L

K

P

P

K

K

S

N

F

Y

D

D

Q

Q

D

V

P

P

A

A

N

N

I

K

A

P

L

I

A

V

D

D

A

A

F

I

K

K

A

A

K

K

K

K

E

Q

D

D

P

P

S

S

G

G

P

A

F
|
F

V

V

F

W

P

T

S

T

Y

Y

S

A

A

A

V

L

E

Q

V

S

I

L

A

Q

E

A

G

G

I

L

K

N

A

-

A

-

K

Q

S

S

E

D

D

D

A

P

T

A

K

E

V

I

A

A

E

K

A

Y

I

L

H

K

A

A

G

N

T

S

F

V

K

D

T

T

P

V

T

M

G

G

D

P

L

L

S

T

F

W

D

D

A

E

K

K

G

G

D

D

L

L

K

K

D

G

F

F

K

E

F

F

V

G

V

V

Y

F

E

D

W

W

H

H

binding isoleucine: Y18 (= Y45), L77 (= L104), C78 (= C105), S79 (= S106), A100 (= A127), A101 (= A128), T102 (= T129), Y150 (= Y177), Y202 (= Y229), E226 (= E253), F276 (= F303)

1z16A Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound leucine (see paper)
60% identity, 89% coverage: 30:364/375 of query aligns to 3:335/344 of 1z16A

query
sites
1z16A

I

I

K

K

I

V

G

A

I

V

A

V

G

G

P

A

K

M

T

S

G

G

P

P

V

V

A

A

Q

Q

Y
|
Y

G

G

D

D

M

Q

Q

E

F

F

S

T

G

G

A

A

K

E

M

Q

A

A

I

V

E

A

Q

D

I

I

N

N

A

A

K

K

G

G

G

G

V

I

D

K

G

G

K

N

K

K

L

L

E

Q

A

I

V

V

E

K

Y

Y

D

D

D

D

A

A

C

C

D

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

V

V

V

V

N

N

D

D

G

G

V

I

K

K

F

Y

V

V

V

I

G

G

H

H

L

L

C
|
C

S
|
S

S

S

S

S

T

T

Q

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

D

D

E

E

G

G

V

I

I

L

M

M

I

I

T

T

P

P

A
|
A

A
|
A

T
|
T

S

A

P

P

D

E

I

L

T

T

A

A

R

R

G

G

Y

Y

K

Q

M

L

I

I

F

L

R

R

T

T

I

T

G

G

L

L

D

D

S

S

A

D

Q

Q

G

G

P

P

A

T

A

A

G

A

N

K

Y

Y

I

I

A

L

D

E

H

K

V

V

K

K

P

P

K

Q

I

R

V

I

G

A

V

I

L

V

H

H

D

D

K

K

Q

Q

Q

Q

Y
|
Y

G

G

E

E

G

G

I

L

A

A

T

R

A

A

V

V

K

Q

S

D

T

G

L

L

E

K

K

K

K

G

G

N

T

A

K

N

V

V

A

V

V

F

F

F

E

D

G

G

V

I

N

T

A

A

G

G

D

E

K

K

D

D

F

F

S

S

A

T

I

L

I

V

A

A

K

R

L

L

K

K

Q

K

A

E

N

N

V

I

D

D

F

F

V

V

Y

Y

Y

Y

G

G

G

G

Y
|
Y

H

H

P

P

E

E

L

M

G

G

L

Q

I

I

L

L

R

R

Q

Q

A

A

Q

R

E

A

K

A

G

G

L

L

K

K

A

T

K

Q

F

F

M

M

G

G

P

P

E
|
E

G

G

V

V

G

A

N

N

D

V

S

S

I

L

T

S

Q

N

I

I

A

A

K

G

D

E

A

S

A

A

E

E

G

G

L

L

L

L

V

V

T

T

L

K

P

P

K

K

S

N

F

Y

D

D

Q

Q

D

V

P

P

A

A

N

N

I

K

A

P

L

I

A

V

D

D

A

A

F

I

K

K

A

A

K

K

K

K

E

Q

D

D

P

P

S

S

G

G

P

A

F
|
F

V

V

F

W

P

T

S

T

Y

Y

S

A

A

A

V

L

E

Q

V

S

I

L

A

Q

E

A

G

G

I

L

K

N

A

-

A

-

K

Q

S

S

E

D

D

D

A

P

T

A

K

E

V

I

A

A

E

K

A

Y

I

L

H

K

A

A

G

N

T

S

F

V

K

D

T

T

P

V

T

M

G

G

D

P

L

L

S

T

F

W

D

D

A

E

K

K

G

G

D

D

L

L

K

K

D

G

F

F

K

E

F

F

V

G

V

V

Y

F

E

D

W

W

H

H

binding leucine: Y18 (= Y45), C78 (= C105), S79 (= S106), A100 (= A127), A101 (= A128), T102 (= T129), Y150 (= Y177), Y202 (= Y229), E226 (= E253), F276 (= F303)

3ip9A Structure of atu2422-gaba receptor in complex with gaba (see paper)
46% identity, 90% coverage: 30:366/375 of query aligns to 3:338/348 of 3ip9A

query
sites
3ip9A

I

V

K

V

I

I

G

A

I

V

A

G

G

A

P

P

K

L

T

T

G

G

P

P

V

N

A

A

Q

A

Y

F

G

G

D

A

M

Q

Q

I

F

Q

S

K

G

G

A

A

K

E

M

Q

A

A

I

A

E

K

Q

D

I

I

N

N

A

A

K

A

G

G

G

G

V

I

D

N

G

G

K

E

K

Q

L

I

E

K

A

I

V

V

E

L

Y

G

D

D

D

D

A

V

C

S

D

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

V

F

V

V

N

A

D

D

G

G

V

V

K

K

F

F

V

V

V

V

G

G

H

H

L

F

C
x
N

S
|
S

S

G

S

V

T

S

Q

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

D

E

E

N

G

G

V

I

I

L

M

E

I

I

T

T

P

P

A
|
A

A
|
A

T
|
T

S

N

P

P

D

V

I

F

T

T

A

E

R

R

G

G

Y

L

K

W

M

N

I

T

F

F

R

R

T

T

I

C

G

G

L

R

D

D

S

D

A

Q

Q

Q

G

G

P

G

A

I

A

A

G

G

N

K

Y

Y

I

L

A

A

D

D

H

H

V

F

K

K

P

D

K

A

I

K

V

V

G

A

V

I

L

I

H

H

D

D

K

K

Q

T

Q

P

Y
|
Y

G

G

E

Q

G

G

I

L

A

A

T

D

A

E

V

T

K

K

S

K

T

A

L

A

E

N

K

A

K

A

G

G

T

V

K

T

V

E

A

V

V

M

F

Y

E

E

G

G

V

V

N

N

A

V

G

G

D

D

K

K

D

D

F

F

S

S

A

A

I

L

I

I

A

S

K

K

L

M

K

K

Q

E

A

A

N

G

V

V

D

S

F

I

V

I

Y

Y

Y

W

G

G

G

G

Y

L

H

H

P

T

E

E

L

A

G

G

L

L

I

I

L

I

R

R

Q

Q

A

A

Q

A

E

D

K

Q

G

G

L

L

K

K

A

A

K

K

F

L

M

V

G

S

P

G

E
x
D

G

G

V

I

G

V

N

S

D

N

S

E

I

L

T

A

Q

S

I

I

A

A

K

G

D

D

A

A

A

V

E

E

G

G

L

T

L

L

V

N

T

T

L

F

P

G

K

P

S

D

F

P

D

T

Q

L

D

R

P

P

A

E

N

N

I

K

A

E

L

L

A

V

D

E

A

K

F

F

K

K

A

A

K

A

K

G

E

F

D

N

P

P

S

E

G

A

P

-

F
x
Y

V

T

F

L

P

Y

S

S

Y

Y

S

A

A

A

V

M

E

Q

V

A

I

I

A

A

E

G

G

A

I

A

K

K

A

A

A

A

K

G

S

S

E

V

D

E

A

P

T

E

K

K

V

V

A

A

E

E

A

A

I

L

H

K

A

K

G

G

T

S

F

F

K

P

T

T

P

A

T

L

G

G

D

E

L

I

S

S

F

F

D

D

A

E

K

K

G

G

D

D

L

P

K

K

D

L

F

P

K

G

F

Y

V

V

V

M

Y

Y

E

E

W

W

H

K

N

K

G

G

binding gamma-amino-butanoic acid: N78 (≠ C105), S79 (= S106), A100 (= A127), A101 (= A128), T102 (= T129), Y150 (= Y177), D226 (≠ E253), Y275 (≠ F303)

3ip7A Structure of atu2422-gaba receptor in complex with valine (see paper)
46% identity, 90% coverage: 30:366/375 of query aligns to 3:338/348 of 3ip7A

query
sites
3ip7A

I

V

K

V

I

I

G

A

I

V

A

G

G

A

P

P

K

L

T

T

G

G

P

P

V

N

A

A

Q

A

Y

F

G

G

D

A

M

Q

Q

I

F

Q

S

K

G

G

A

A

K

E

M

Q

A

A

I

A

E

K

Q

D

I

I

N

N

A

A

K

A

G

G

G

G

V

I

D

N

G

G

K

E

K

Q

L

I

E

K

A

I

V

V

E

L

Y

G

D

D

D

D

A

V

C

S

D

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

V

F

V

V

N

A

D

D

G

G

V

V

K

K

F

F

V

V

V

V

G

G

H

H

L
x
F

C
x
N

S
|
S

S
x
G

S

V

T

S

Q

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

D

E

E

N

G

G

V

I

I

L

M

E

I

I

T

T

P

P

A
|
A

A
|
A

T
|
T

S

N

P

P

D

V

I

F

T

T

A

E

R

R

G

G

Y

L

K

W

M

N

I

T

F

F

R

R

T

T

I

C

G

G

L

R

D

D

S

D

A

Q

Q

Q

G

G

P

G

A

I

A

A

G

G

N

K

Y

Y

I

L

A

A

D

D

H

H

V

F

K

K

P

D

K

A

I

K

V

V

G

A

V

I

L

I

H

H

D

D

K

K

Q
x
T

Q
x
P

Y
|
Y

G

G

E

Q

G

G

I

L

A

A

T

D

A

E

V

T

K

K

S

K

T

A

L

A

E

N

K

A

K

A

G

G

T

V

K

T

V

E

A

V

V

M

F

Y

E

E

G

G

V

V

N

N

A

V

G

G

D

D

K

K

D

D

F

F

S

S

A

A

I

L

I

I

A

S

K

K

L

M

K

K

Q

E

A

A

N

G

V

V

D

S

F

I

V

I

Y

Y

Y

W

G

G

G

G

Y

L

H

H

P

T

E

E

L

A

G

G

L

L

I

I

L

I

R

R

Q

Q

A

A

Q

A

E

D

K

Q

G

G

L

L

K

K

A

A

K

K

F

L

M

V

G

S

P

G

E
x
D

G

G

V

I

G

V

N

S

D

N

S

E

I

L

T

A

Q

S

I

I

A

A

K

G

D

D

A

A

A

V

E

E

G

G

L

T

L

L

V

N

T

T

L

F

P

G

K

P

S

D

F

P

D

T

Q

L

D

R

P

P

A

E

N

N

I

K

A

E

L

L

A

V

D

E

A

K

F

F

K

K

A

A

K

A

K

G

E

F

D

N

P

P

S

E

G

A

P

-

F

Y

V

T

F

L

P

Y

S

S

Y

Y

S

A

A

A

V

M

E

Q

V

A

I

I

A

A

E

G

G

A

I

A

K

K

A

A

A

A

K

G

S

S

E

V

D

E

A

P

T

E

K

K

V

V

A

A

E

E

A

A

I

L

H
x
K

A

K

G

G

T

S

F

F

K

P

T

T

P

A

T

L

G

G

D

E

L

I

S

S

F
|
F

D

D

A

E

K

K

G

G

D

D

L

P

K
|
K

D

L

F

P

K

G

F

Y

V

V

V

M

Y

Y

E

E

W

W

H

K

N

K

G

G

binding calcium ion: S79 (= S106), G80 (≠ S107), T148 (≠ Q175), P149 (≠ Q176), Y150 (= Y177), K306 (≠ H334), F319 (= F347), K326 (= K354)
binding valine: F77 (≠ L104), N78 (≠ C105), S79 (= S106), A100 (= A127), A101 (= A128), T102 (= T129), Y150 (= Y177), D226 (≠ E253)

3ip6A Structure of atu2422-gaba receptor in complex with proline (see paper)
46% identity, 90% coverage: 30:366/375 of query aligns to 3:338/348 of 3ip6A

query
sites
3ip6A

I

V

K

V

I

I

G

A

I

V

A

G

G

A

P

P

K

L

T

T

G

G

P

P

V

N

A

A

Q

A

Y

F

G

G

D

A

M

Q

Q

I

F

Q

S

K

G

G

A

A

K

E

M

Q

A

A

I

A

E

K

Q

D

I

I

N

N

A

A

K

A

G

G

G

G

V

I

D

N

G

G

K

E

K

Q

L

I

E

K

A

I

V

V

E

L

Y

G

D

D

D

D

A

V

C

S

D

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

V

F

V

V

N

A

D

D

G

G

V

V

K

K

F

F

V

V

V

V

G

G

H

H

L
x
F

C
x
N

S
|
S

S

G

S

V

T

S

Q

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

D

E

E

N

G

G

V

I

I

L

M

E

I

I

T

T

P

P

A
|
A

A
|
A

T
|
T

S

N

P

P

D

V

I

F

T

T

A

E

R

R

G

G

Y

L

K

W

M

N

I

T

F

F

R

R

T

T

I

C

G

G

L

R

D

D

S

D

A

Q

Q

Q

G

G

P

G

A

I

A

A

G

G

N

K

Y

Y

I

L

A

A

D

D

H

H

V

F

K

K

P

D

K

A

I

K

V

V

G

A

V

I

L

I

H

H

D

D

K

K

Q

T

Q

P

Y
|
Y

G

G

E

Q

G

G

I

L

A

A

T

D

A

E

V

T

K

K

S

K

T

A

L

A

E

N

K

A

K

A

G

G

T

V

K

T

V

E

A

V

V

M

F

Y

E

E

G

G

V

V

N

N

A

V

G

G

D

D

K

K

D

D

F

F

S

S

A

A

I

L

I

I

A

S

K

K

L

M

K

K

Q

E

A

A

N

G

V

V

D

S

F

I

V

I

Y

Y

Y

W

G

G

G

G

Y

L

H

H

P

T

E

E

L

A

G

G

L

L

I

I

L

I

R

R

Q

Q

A

A

Q

A

E

D

K

Q

G

G

L

L

K

K

A

A

K

K

F

L

M

V

G

S

P

G

E
x
D

G

G

V

I

G

V

N

S

D

N

S

E

I

L

T

A

Q

S

I

I

A

A

K

G

D

D

A

A

A

V

E

E

G

G

L

T

L

L

V

N

T

T

L

F

P

G

K

P

S

D

F

P

D

T

Q

L

D

R

P

P

A

E

N

N

I

K

A

E

L

L

A

V

D

E

A

K

F

F

K

K

A

A

K

A

K

G

E

F

D

N

P

P

S

E

G

A

P

-

F
x
Y

V

T

F

L

P

Y

S

S

Y

Y

S

A

A

A

V

M

E

Q

V

A

I

I

A

A

E

G

G

A

I

A

K

K

A

A

A

A

K

G

S

S

E

V

D

E

A

P

T

E

K

K

V

V

A

A

E

E

A

A

I

L

H

K

A

K

G

G

T

S

F

F

K

P

T

T

P

A

T

L

G

G

D

E

L

I

S

S

F

F

D

D

A

E

K

K

G

G

D

D

L

P

K

K

D

L

F

P

K

G

F

Y

V

V

V

M

Y

Y

E

E

W

W

H

K

N

K

G

G

binding proline: F77 (≠ L104), N78 (≠ C105), S79 (= S106), A100 (= A127), A101 (= A128), T102 (= T129), Y150 (= Y177), D226 (≠ E253), Y275 (≠ F303)

3ip5A Structure of atu2422-gaba receptor in complex with alanine (see paper)
46% identity, 90% coverage: 30:366/375 of query aligns to 3:338/348 of 3ip5A

query
sites
3ip5A

I

V

K

V

I

I

G

A

I

V

A

G

G

A

P

P

K

L

T

T

G

G

P

P

V

N

A

A

Q

A

Y

F

G

G

D

A

M

Q

Q

I

F

Q

S

K

G

G

A

A

K

E

M

Q

A

A

I

A

E

K

Q

D

I

I

N

N

A

A

K

A

G

G

G

G

V

I

D

N

G

G

K

E

K

Q

L

I

E

K

A

I

V

V

E

L

Y

G

D

D

D

D

A

V

C

S

D

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

V

F

V

V

N

A

D

D

G

G

V

V

K

K

F

F

V

V

V

V

G

G

H

H

L
x
F

C
x
N

S
|
S

S

G

S

V

T

S

Q

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

D

E

E

N

G

G

V

I

I

L

M

E

I

I

T

T

P

P

A
|
A

A
|
A

T
|
T

S

N

P

P

D

V

I

F

T

T

A

E

R

R

G

G

Y

L

K

W

M

N

I

T

F

F

R

R

T

T

I

C

G

G

L

R

D

D

S

D

A

Q

Q

Q

G

G

P

G

A

I

A

A

G

G

N

K

Y

Y

I

L

A

A

D

D

H

H

V

F

K

K

P

D

K

A

I

K

V

V

G

A

V

I

L

I

H

H

D

D

K

K

Q

T

Q

P

Y
|
Y

G

G

E

Q

G

G

I

L

A

A

T

D

A

E

V

T

K

K

S

K

T

A

L

A

E

N

K

A

K

A

G

G

T

V

K

T

V

E

A

V

V

M

F

Y

E

E

G

G

V

V

N

N

A

V

G

G

D

D

K

K

D

D

F

F

S

S

A

A

I

L

I

I

A

S

K

K

L

M

K

K

Q

E

A

A

N

G

V

V

D

S

F

I

V

I

Y

Y

Y

W

G

G

G

G

Y

L

H

H

P

T

E

E

L

A

G

G

L

L

I

I

L

I

R

R

Q

Q

A

A

Q

A

E

D

K

Q

G

G

L

L

K

K

A

A

K

K

F

L

M

V

G

S

P

G

E
x
D

G

G

V

I

G

V

N

S

D

N

S

E

I

L

T

A

Q

S

I

I

A

A

K

G

D

D

A

A

A

V

E

E

G

G

L

T

L

L

V

N

T

T

L

F

P

G

K

P

S

D

F

P

D

T

Q

L

D

R

P

P

A

E

N

N

I

K

A

E

L

L

A

V

D

E

A

K

F

F

K

K

A

A

K

A

K

G

E

F

D

N

P

P

S

E

G

A

P

-

F

Y

V

T

F

L

P

Y

S

S

Y

Y

S

A

A

A

V

M

E

Q

V

A

I

I

A

A

E

G

G

A

I

A

K

K

A

A

A

A

K

G

S

S

E

V

D

E

A

P

T

E

K

K

V

V

A

A

E

E

A

A

I

L

H

K

A

K

G

G

T

S

F

F

K

P

T

T

P

A

T

L

G

G

D

E

L

I

S

S

F

F

D

D

A

E

K

K

G

G

D

D

L

P

K

K

D

L

F

P

K

G

F

Y

V

V

V

M

Y

Y

E

E

W

W

H

K

N

K

G

G

binding alanine: F77 (≠ L104), N78 (≠ C105), S79 (= S106), A100 (= A127), A101 (= A128), T102 (= T129), Y150 (= Y177), D226 (≠ E253)

3ipcA Structure of atu2422-gaba f77a mutant receptor in complex with leucine (see paper)
46% identity, 90% coverage: 30:366/375 of query aligns to 3:338/348 of 3ipcA

query
sites
3ipcA

I

V

K

V

I

I

G

A

I

V

A

G

G

A

P

P

K

L

T

T

G

G

P

P

V

N

A

A

Q

A

Y

F

G

G

D

A

M

Q

Q

I

F

Q

S

K

G

G

A

A

K

E

M

Q

A

A

I

A

E

K

Q

D

I

I

N

N

A

A

K

A

G

G

G

G

V

I

D

N

G

G

K

E

K

Q

L

I

E

K

A

I

V

V

E

L

Y

G

D

D

D

D

A

V

C

S

D

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

V

F

V

V

N

A

D

D

G

G

V

V

K

K

F

F

V

V

V

V

G

G

H

H

L
x
A

C
x
N

S
|
S

S

G

S

V

T

S

Q

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

D

E

E

N

G

G

V

I

I

L

M

E

I

I

T

T

P

P

A
|
A

A
|
A

T
|
T

S

N

P

P

D

V

I

F

T

T

A

E

R

R

G

G

Y

L

K

W

M

N

I

T

F

F

R

R

T

T

I

C

G

G

L

R

D

D

S

D

A

Q

Q

Q

G

G

P

G

A

I

A

A

G

G

N

K

Y

Y

I

L

A

A

D

D

H

H

V

F

K

K

P

D

K

A

I

K

V

V

G

A

V

I

L

I

H

H

D

D

K

K

Q

T

Q

P

Y
|
Y

G

G

E

Q

G

G

I

L

A

A

T

D

A

E

V

T

K

K

S

K

T

A

L

A

E

N

K

A

K

A

G

G

T

V

K

T

V

E

A

V

V

M

F

Y

E

E

G

G

V

V

N

N

A

V

G

G

D

D

K

K

D

D

F

F

S

S

A

A

I

L

I

I

A

S

K

K

L

M

K

K

Q

E

A

A

N

G

V

V

D

S

F

I

V

I

Y

Y

Y

W

G

G

G

G

Y

L

H

H

P

T

E

E

L

A

G

G

L

L

I

I

L

I

R

R

Q

Q

A

A

Q

A

E

D

K

Q

G

G

L

L

K

K

A

A

K

K

F

L

M

V

G

S

P

G

E
x
D

G

G

V

I

G

V

N

S

D

N

S

E

I

L

T

A

Q

S

I

I

A

A

K

G

D

D

A

A

A

V

E

E

G

G

L

T

L

L

V

N

T

T

L

F

P

G

K

P

S

D

F

P

D

T

Q

L

D

R

P

P

A

E

N

N

I

K

A

E

L

L

A

V

D

E

A

K

F

F

K

K

A

A

K

A

K

G

E

F

D

N

P

P

S

E

G

A

P

-

F

Y

V

T

F

L

P

Y

S

S

Y

Y

S

A

A

A

V

M

E

Q

V

A

I

I

A

A

E

G

G

A

I

A

K

K

A

A

A

A

K

G

S

S

E

V

D

E

A

P

T

E

K

K

V

V

A

A

E

E

A

A

I

L

H

K

A

K

G

G

T

S

F

F

K

P

T

T

P

A

T

L

G

G

D

E

L

I

S

S

F

F

D

D

A

E

K

K

G

G

D

D

L

P

K

K

D

L

F

P

K

G

F

Y

V

V

V

M

Y

Y

E

E

W

W

H

K

N

K

G

G

binding leucine: A77 (≠ L104), N78 (≠ C105), S79 (= S106), A100 (= A127), A101 (= A128), T102 (= T129), Y150 (= Y177), D226 (≠ E253)

4n0qB Crystal structure of an abc transporter, substrate-binding protein from brucella melitensis 16m in complex with l-leucine using a crystal grown in a crystal former (microlytic)
42% identity, 90% coverage: 30:367/375 of query aligns to 3:341/345 of 4n0qB

query
sites
4n0qB

I

I

K

T

I

I

G

G

I

V

A

I

G

A

P

P

K

L

T

T

G

G

P

P

V

V

A

A

Q

A

Y
x
F

G

G

D

D

M

Q

Q

V

F

K

S

K

G

G

A

A

K

E

M

T

A

A

I

V

E

E

Q

V

I

I

N

N

A

K

K

A

G

G

G

G

V

I

D

K

G

G

K

E

K

K

L

V

E

V

A

L

V

K

E

F

Y

A

D

D

D

D

A

A

C

G

D

E

P

P

K

K

Q

Q

A

G

V

V

A

S

V

A

A

A

N

N

K

Q

V

I

V

V

N

G

D

D

G

G

V

I

K

K

F

F

V

V

V

V

G

G

H

L

L
x
V

C
x
T

S
x
T

S

G

S

V

T

A

Q

V

P

P

A

V

S

S

D

D

I

V

Y

L

E

S

D

E

E

N

G

G

V

V

I

L

M

M

I

V

T

T

P

P

A
x
T

A
|
A

T
|
T

S

G

P

P

D

D

I

L

T

T

A

A

R

R

G

G

Y

L

K

E

M

N

I

V

F

F

R

R

T

T

I

C

G

G

L

R

D

D

S

G

A

Q

Q

Q

G

A

P

E

A

V

A

M

G

A

N

D

Y

Y

I

V

A

L

D

K

H

N

V

M

K

K

P

D

K

K

I

K

V

V

G

A

V

V

L

I

H

H

D

D

K

K

Q

G

Q

A

Y
|
Y

G

G

E

K

G

G

I

L

A

A

T

D

A

A

V

F

K

K

S

A

T

A

L

I

E

N

K

K

K

G

G

G

T

I

K

T

V

E

A

V

V

H

F

Y

E

D

G

S

V

V

N

T

A

P

G

G

D

D

K

K

D

D

F

F

S

S

A

A

I

L

I

V

A

T

K

K

L

L

K

K

Q

S

A

A

N

G

V

A

D

E

F

V

V

V

Y

Y

Y

F

G

G

G

G

Y
|
Y

H

H

P

A

E

E

L

G

G

G

L

L

I

L

L

S

R

R

Q

Q

A

L

Q

H

E

D

K

A

G

G

L

M

K

Q

A

A

K

L

F

V

M

L

G

G

P

G

E
|
E

G

G

V

L

G

S

N

N

D

T

S

E

I

Y

T

W

Q

A

I

I

A

G

K

G

D

T

A

N

A

A

E

Q

G

G

L

T

L

L

V

F

T

T

L

N

P

A

K

K

S

D

F

A

D

T

Q

K

D

N

P

P

A

A

N

A

I

K

A

D

L

A

A

I

D

Q

A

A

F

L

K

K

A

A

K

K

K

N

E

I

D

-

P

P

S

A

G

E

P

A

F
|
F

V

T

F

M

P

N

S

A

Y

Y

S

A

A

A

V

V

E

E

V

V

I

I

A

K

E

A

G

G

I

I

-

E

K

R

A

A

A

G

K

S

S

T

E

D

D

D

A

S

T

A

K

A

V

V

A

A

E

K

A

A

I

L

H

H

A

D

G

G

T

K

-

P

F

I

K

E

T

T

P

A

T

I

G

G

D

T

L

L

S

T

F

Y

D

S

A

E

K

T

G

G

D

D

L

L

K

S

D

S

F

P

K

S

F

F

V

D

V

I

Y

F

E

K

W

W

H

D

N

D

G

G

K

K

binding leucine: F18 (≠ Y45), V77 (≠ L104), T78 (≠ C105), T79 (≠ S106), T100 (≠ A127), A101 (= A128), T102 (= T129), Y150 (= Y177), Y202 (= Y229), E226 (= E253), F275 (= F303)

4gnrA 1.0 angstrom resolution crystal structure of the branched-chain amino acid transporter substrate binding protein livj from streptococcus pneumoniae str. Canada mdr_19a in complex with isoleucine
32% identity, 86% coverage: 29:349/375 of query aligns to 2:322/348 of 4gnrA

query
sites
4gnrA

T

T

I

I

K

K

I

I

G

G

I

F

A

N

G

F

P

E

K

E

T

S

G

G

P

S

V

L

A

A

Q

A

Y
|
Y

G

G

D

T

M

A

Q

E

F

Q

S

K

G

G

A

A

K

Q

M

L

A

A

I

V

E

D

Q

E

I

I

N

N

A

A

K

A

G

G

G

G

V

I

D

D

G

G

K

K

K

Q

L

I

E

E

A

V

V

V

E

D

Y

K

D

D

D

N

A

K

C

S

D

E

P

T

K

A

Q

E

A

A

V

A

A

S

V

V

-

T

A

T

N

N

K

L

V

V

V

T

N

Q

D

S

G

K

V

V

K

S

F

A

V

V

V

V

G

G

H

P

L
x
A

C
x
T

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|
S

S

G

S

A

T

T

Q

A

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A

A

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V

D

A

I

N

Y

A

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D

K

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A

G

G

V

V

I

P

M

L

I

I

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x
S

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|
A

T
|
T

S

Q

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D

D

G

I

L

T

T

A

-

R

K

G

G

Y

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K

D

M

Y

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F

F

R

I

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G

I

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D

D

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S

A

F

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Q

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G

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K

A

I

A

I

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N

N

Y

Y

I

V

A

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D

E

H

K

V

L

K

N

P

A

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K

I

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V

V

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V

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L

Y

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D

D

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N

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A

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Y
|
Y

G

A

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K

G

G

I

I

A

A

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-

A

-

V

-

K

K

S

S

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F

L

R

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E

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Y

G

K

T

G

K

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V

I

A

V

V

A

F

D

E

E

G

T

V

F

N

V

A

A

G

G

D

D

K

T

D

D

F

F

S

Q

A

A

I

A

I

L

A

T

K

K

L

M

K

K

Q

G

A

K

N

D

V

F

D

D

F

A

V

I

Y

V

Y

V

G

P

G

G

Y
|
Y

H

Y

P

N

E

E

L

A

G

G

L

K

I

I

L

V

R

N

Q

Q

A

A

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R

E

G

K

M

G

G

L

I

K

D

A

K

K

P

F

I

M

V

G

G

P

G

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x
D

G

G

V

F

G

N

N

G

D

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S

E

I

F

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Q

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Q

-

A

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D

K

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V

D

S

P

A

A

K

N

A

I

K

A

A

L

F

A

L

D

D

A

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F

Y

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R

A

A

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K

K

Y

E

N

D

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P

E

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P

G

S

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T

F
|
F

V

A

F

A

P

L

S

A

Y

Y

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D

A

S

V

V

E

H

V

L

I

V

A

A

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N

G

A

I

A

K

K

A

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A

A

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K

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N

E

S

D

G

A

E

T

I

K

K

V

D

A

N

E

L

A

A

I

-

H

K

A

T

G

K

T

D

F

F

K

E

T

G

P

V

T

T

G

G

D

Q

L

T

S

S

F

F

D

D

A

A

binding isoleucine: Y18 (= Y45), A78 (≠ L104), T79 (≠ C105), S80 (= S106), S101 (≠ A127), A102 (= A128), T103 (= T129), Y151 (= Y177), Y200 (= Y229), D224 (≠ E253), F277 (= F303)

3td9A Crystal structure of a leucine binding protein livk (tm1135) from thermotoga maritima msb8 at 1.90 a resolution
29% identity, 86% coverage: 29:352/375 of query aligns to 1:324/350 of 3td9A

query
sites
3td9A

T

V

I

V

K

K

I

I

G

A

I

V

A

I

G

L

P

P

K

M

T

T

G

G

P

G

V

I

A

S

Q

A

Y
x
F

G

G

D

R

M

M

Q

V

F

W

S

E

G

G

A

I

K

Q

M

I

A

A

I

H

E

E

Q

E

I

-

N

-

A

-

K

K

G

P

G

T

V

V

D

L

G

G

K

E

K

E

L

V

E

E

A

L

V

V

E

L

Y

L

D

D

D

T

A

R

C

S

D

E

P

K

K

T

Q

E

A

A

V

A

A

N

V

A

A

A

N

A

K

R

V

A

V

I

N

D

-

K

D

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G

K

V

V

K

L

F

A

V

I

V

I

G

G

H

E

L
x
V

C
x
A

S
|
S

S

A

S

H

T

S

Q

L

P

A

A

I

S

A

D

P

I

I

Y

A

E

E

D

E

E

N

G

K

V

V

I

P

M

M

I

V

T

T

P

P

A
|
A

A
x
S

T
|
T

S

N

P

P

D

L

I

V

T

T

A

-

R

Q

G

G

Y

R

K

K

M

F

I

V

F

S

R

R

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V

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C

G

F

L

I

D

D

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P

A

F

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Q

G

G

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A

A

A

A

M

G

A

N

V

Y

F

I

A

A

Y

D

K

H

N

V

L

K

G

P

A

K

K

I

R

V

V

G

V

V

V

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F

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D

D

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K

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Q

Q

Q

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Y
|
Y

G

S

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V

G

G

I

L

A

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N

A

F

V

F

K

I

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T

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L

F

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K

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L

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G

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G

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V

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K

A

R

V

V

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F

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G

-

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F

N

R

A

S

G

G

D

D

K

Q

D

D

F

F

S

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A

A

I

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I

L

A

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K

V

L

A

K

M

Q

S

A

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N

N

V

P

D

D

F

A

V

I

Y

Y

Y

I

G

T

G

G

Y
|
Y

H

Y

P

P

E

E

L

I

G

A

L

L

I

I

L

S

R

R

Q

Q

A

A

Q

R

E

Q

K

L

G

G

L

F

K

T

A

G

K

Y

F

I

M

L

G

A

P

G

E
x
D

G

G

V

A

G

D

N

A

D

P

S

E

I

L

T

I

Q

E

I

I

A

G

K

G

D

E

A

A

A

V

E

E

G

G

L

L

L

L

V

F

T

T

L

T

-
x
H

-

Y

-

H

P

P

K

K

S

A

F

-

D

A

Q

S

D

N

P

P

A

V

N

A

I

K

A

K

L

F

A

V

D

E

A

V

F

Y

K

K

A

E

K

K

K

Y

E

G

D

K

P

E

S

P

G

A

P

A

F
x
L

V

N

F

A

P

L

S

G

Y

Y

S

D

A

A

V

Y

E

M

V

V

I

L

A

L

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D

G

A

I

I

K

E

A

R

A

A

K

G

S

S

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F

D

D

A

R

T

E

K

K

V

I

A

A

E

E

A

E

I

I

H

R

-

K

A

T

G

R

T

N

F

F

K

N

T

G

P

A

T

S

G

G

D

I

L

I

S

N

F

I

D

D

A

E

K

N

G

G

D

D

binding phenylalanine: F17 (≠ Y45), V74 (≠ L104), A75 (≠ C105), S76 (= S106), A97 (= A127), S98 (≠ A128), T99 (= T129), Y147 (= Y177), Y198 (= Y229), D222 (≠ E253), H245 (vs. gap), L274 (≠ F303)

4q6bA Crystal structure of abc transporter substrate-binding protein fromdesulfitobacterium hafniense complex with leu
28% identity, 87% coverage: 28:352/375 of query aligns to 1:316/335 of 4q6bA

query
sites
4q6bA

D

E

T

E

I

I

K

F

I

I

G

G

I

V

A

A

G

W

P

P

K

-

T

-

G

-

P

-

V

F

A

A

Q

S

Y

L

G

D

D

D

M

L

Q

F

F

A

S

E

G

G

A

L

K

E

M

L

A

A

I

V

E

Q

Q

E

I

I

N

N

A

E

K

Q

G

G

G

G

V

V

D

Q

G

G

K

R

K

K

L

L

E

S

A

L

V

V

E

K

Y

A

D

D

D

D

A

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C

A

D

E

P

L

K

E

Q

K

A

G

V

L

A

A

V

I

A

A

N

Q

K

A

V

F

V

A

-

D

N

N

D

A

G

G

V

I

K

Q

F

A

V

V

V

I

G

G

H

H

L
x
R

C
x
N

S
|
S

S

F

S

I

T

S

Q

I

P

P

A

A

S

A

D

S

I

I

Y

Y

E

D

D

Q

E

A

G

G

V

L

I

V

M

M

I

L

T

S

P

P

A
|
A

A
x
S

T
|
T

S

S

P

P

D

D

I

L

T

T

A

D

R

H

G

G

Y

Y

K

I

M

H

I

V

F

F

R

R

T

N

I

I

G

P

L

S

D

D

S

Q

A

E

Q

I

G

A

P

R

A

Q

A

L

G

A

N

I

Y

Y

I

L

A

A

D

E

H

Q

V

G

K

H

P

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K

R

I

M

V

V

G

-

V

I

L

Y

H

Y

D

T

K

D

Q

D

Q

S

Y

Y

G

G

E

N

G

G

I

L

A

A

T

N

A

A

V

F

K

E

S

D

T

Y

L

A

E

R

K

A

K

Q

G