SitesBLAST

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
42% identity, 62% coverage: 9:240/372 of query aligns to 7:234/358 of 8y5iA

query
sites
8y5iA

G

G

L

I

R

R

V

K

V

C

Y
x
F

P

D

G

G

G
x
K

R

E

G

-

A

-

G

-

P

-

V

-

A

V

V

I

D

P

G

Q

V

L

D

D

L

L

E

T

I

I

P

N

A

N

G

G

R

E

I

F

V

L

A

T

L

L

G

G

A

P

S

S

G
|
G

S

C

G

G

K
|
K

S
x
T

L

V

L

L

R

R

A

L

V

I

A

A

G

G

L

L

E

E

P

T

V

V

A

D

A

S

G

G

S

R

I

I

R

M

W

L

D

D

G

N

R

E

D

D

V

I

V

T

G

H

T

V

P

P

V

A

H

E

R

N

R

R

G

Y

F

V

G

N

L

T

M

V

F

F

Q

Q

E

S

G

Y

Q

A

L

L

F

F

P

P

F

H

R

M

D

T

V

V

G

F

G

E

N

N

V

V

A

A

Y

F

G

G

L

L

-

R

-

M

T

Q

G

K

L

T

P

P

R

A

A

A

E

E

R

I

A

T

R

P

R

R

V

V

A

M

E

E

M

A

L

L

E

R

L

M

V

V

G

Q

L

L

P

E

G

T

Y

F

G

A

P

Q

R
|
R

P

K

I

P

T

H

T
x
Q

L

L

S
|
S

G

G

Q

Q

A

Q

Q

Q

R

R

V

V

A

A

L

I

A

A

R

R

A

A

L

V

A

V

P

N

R

K

P

P

R

R

L

L

D

D

E

Q

P

S

L

L

S

S

A

A

L

L

D

D

R

Y

A

K

L

L

R

R

E

K

Q

Q

L

M

A

Q

V

N

D

E

L

L

R

K

A

A

I

L

L

Q

A

R

E

K

Q

L

G

G

T

I

T

T

A

F

L

V

Y

F

V

V

T

T

H

H

D

D

Q

Q

D

E

E

E

A

A

M

L

T

T

V

M

A

S

D

D

E

R

V

I

G

V

V

V

M

M

E

R

A

D

G

G

R

R

L

I

A

E

R

Q

L

D

A

G

A

T

P

P

A

R

E

E

L

I

W

Y

A

E

D

E

P

P

G

K

S

N

A

L

S

F

V

V

A

A

A

G

F

F

L

I

G

G

binding adenosine-5'-triphosphate: F12 (≠ Y14), K15 (≠ G17), G38 (= G46), K41 (= K49), T42 (≠ S50), T43 (≠ S51), R128 (= R134), Q132 (≠ T138), S134 (= S140)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
42% identity, 60% coverage: 18:240/372 of query aligns to 11:235/371 of P68187

query
sites
P68187

R

K

G

A

A

W

G

G

P

E

V

V

A

V

V

S

D

K

G

D

V

I

D

N

L

L

E

D

I

I

P

H

A

E

G

G

R

E

I

F

V

V

A

V

L

F

L

V

G

G

A

P

S

S

G

G

S

C

G

G

K

K

S

S

S

T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

P

T

V

I

A

T

A

S

G

G

S

D

I

L

R

F

W

I

D

G

G

E

R

K

D

R

V

M

V

N

G

D

T

T

P

P

V

P

H

A

R

E

R

R

G

G

F

V

G

G

L

M

M

V

F

F

Q

Q

E

S

G

Y

Q
x
A

L

L

F

Y

P

P

F

H

R

L

D

S

V

V

G

A

G

E

N

N

V

M

A

S

Y

F

G

G

L

L

T

K

-

L

-

A

G

G

L

A

P
x
K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

A
x
V

R

N

R

Q

V
|
V

A

A

E
|
E

M

V

L

L

E

Q

L

L

V
x
A

G

H

L

L

P

L

G

D

Y

R

G

K

P

P

R

K

P

-

I

-

T

-

T

A

L

L

S

S

G

G

G
|
G

Q

Q

A

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

R

E

P

P

R

S

L

V

L

F

L

L

D
|
D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

R

A

A

A

L

L

R

R

E

V

Q

Q

L

M

A

R

V

I

D

E

L

I

R

S

A

R

I

L

L

H

A

K

E

R

Q

L

G

G

T

R

T

T

A

M

L

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

M

M

T

T

V

L

A

A

D

D

E

K

V

I

G

V

V

V

M

L

E

D

A

A

G

G

R

R

L

V

A

A

R

Q

L

V

A

G

A

K

P

P

A

L

E

E

L

L

W

Y

A

H

D

Y

P

P

G

A

S

D

A
x
R

S

F

V

V

A

A

A

G

F

F

L

I

G

G

A85 (≠ Q92) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ P111) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ A116) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (= V119) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E121) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ V126) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G142) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D163) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ A233) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
42% identity, 60% coverage: 18:240/372 of query aligns to 10:234/371 of 3puyA

query
sites
3puyA

R

K

G

A

A
x
W

G

G

P

E

V

V

A

V

V

S

D

K

G

D

V

I

D

N

L

L

E

D

I

I

P

H

A

E

G

G

R

E

I

F

V

V

A

V

L

F

L

V

G

G

A

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

P

T

V

I

A

T

A

S

G

G

S

D

I

L

R

F

W

I

D

G

G

E

R

K

D

R

V

M

V

N

G

D

T

T

P

P

V

P

H

A

R

E

R

R

G

G

F

V

G

G

L

M

M

V

F

F

Q
|
Q

E

S

G

Y

Q

A

L

L

F

Y

P

P

F

H

R

L

D

S

V

V

G

A

G

E

N

N

V

M

A

S

Y

F

G

G

L

L

T

K

-

L

-

A

G

G

L

A

P

K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

A

V

R

N

R

Q

V

V

A

A

E

E

M

V

L

L

E

Q

L

L

V

A

G

H

L

L

P

L

G

D

Y
x
R

G

K

P

P

R

K

P

-

I

-

T

-

T
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

A

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

R

E

P

P

R

S

L

V

L

F

L

L

D

D

E
|
E

P

P

L

L

S

S

A

N

L

L

D

D

R

A

A

A

L

L

R

R

E

V

Q

Q

L

M

A

R

V

I

D

E

L

I

R

S

A

R

I

L

L

H

A

K

E

R

Q

L

G

G

T

R

T

T

A

M

L

I

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

V

E

E

A

A

M

M

T

T

V

L

A

A

D

D

E

K

V

I

G

V

V

V

M

L

E

D

A

A

G

G

R

R

L

V

A

A

R

Q

L

V

A

G

A

K

P

P

A

L

E

E

L

L

W

Y

A

H

D

Y

P

P

G

A

S

D

A

R

S

F

V

V

A

A

A

G

F

F

L

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ A20), S37 (= S45), G38 (= G46), C39 (≠ S47), G40 (= G48), K41 (= K49), S42 (= S50), T43 (≠ S51), Q81 (= Q89), R128 (≠ Y131), A132 (≠ T138), S134 (= S140), G136 (= G142), Q137 (= Q143), E158 (= E164), H191 (= H197)
binding magnesium ion: S42 (= S50), Q81 (= Q89)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
42% identity, 60% coverage: 18:240/372 of query aligns to 10:234/371 of 3puxA

query
sites
3puxA

R

K

G

A

A
x
W

G

G

P

E

V

V

A

V

V

S

D

K

G

D

V

I

D

N

L

L

E

D

I

I

P

H

A

E

G

G

R

E

I

F

V

V

A

V

L

F

L

V

G

G

A

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

P

T

V

I

A

T

A

S

G

G

S

D

I

L

R

F

W

I

D

G

G

E

R

K

D

R

V

M

V

N

G

D

T

T

P

P

V

P

H

A

R

E

R

R

G

G

F

V

G

G

L

M

M

V

F

F

Q
|
Q

E

S

G

Y

Q

A

L

L

F

Y

P

P

F

H

R

L

D

S

V

V

G

A

G

E

N

N

V

M

A

S

Y

F

G

G

L

L

T

K

-

L

-

A

G

G

L

A

P

K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

A

V

R

N

R

Q

V

V

A

A

E

E

M

V

L

L

E

Q

L

L

V

A

G

H

L

L

P

L

G

D

Y
x
R

G

K

P

P

R

K

P

-

I

-

T

-

T

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

A

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

R

E

P

P

R

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

R

A

A

A

L

L

R

R

E

V

Q

Q

L

M

A

R

V

I

D

E

L

I

R

S

A

R

I

L

L

H

A

K

E

R

Q

L

G

G

T

R

T

T

A

M

L

I

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

V

E

E

A

A

M

M

T

T

V

L

A

A

D

D

E

K

V

I

G

V

V

V

M

L

E

D

A

A

G

G

R

R

L

V

A

A

R

Q

L

V

A

G

A

K

P

P

A

L

E

E

L

L

W

Y

A

H

D

Y

P

P

G

A

S

D

A

R

S

F

V

V

A

A

A

G

F

F

L

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ A20), G38 (= G46), C39 (≠ S47), G40 (= G48), K41 (= K49), S42 (= S50), T43 (≠ S51), R128 (≠ Y131), S134 (= S140), Q137 (= Q143)
binding beryllium trifluoride ion: S37 (= S45), G38 (= G46), K41 (= K49), Q81 (= Q89), S134 (= S140), G136 (= G142), H191 (= H197)
binding magnesium ion: S42 (= S50), Q81 (= Q89)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
42% identity, 60% coverage: 18:240/372 of query aligns to 10:234/371 of 3puwA

query
sites
3puwA

R

K

G

A

A
x
W

G

G

P

E

V

V

A
x
V

V

S

D

K

G

D

V

I

D

N

L

L

E

D

I

I

P

H

A

E

G

G

R

E

I

F

V

V

A

V

L

F

L

V

G

G

A

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

P

T

V

I

A

T

A

S

G

G

S

D

I

L

R

F

W

I

D

G

G

E

R

K

D

R

V

M

V

N

G

D

T

T

P

P

V

P

H

A

R

E

R

R

G

G

F

V

G

G

L

M

M

V

F

F

Q
|
Q

E

S

G

Y

Q

A

L

L

F

Y

P

P

F

H

R

L

D

S

V

V

G

A

G

E

N

N

V

M

A

S

Y

F

G

G

L

L

T

K

-

L

-

A

G

G

L

A

P

K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

A

V

R

N

R

Q

V

V

A

A

E

E

M

V

L

L

E

Q

L

L

V

A

G

H

L

L

P

L

G

D

Y
x
R

G

K

P

P

R

K

P

-

I

-

T

-

T
x
A

L

L

S
|
S

G
|
G

Q
|
Q

A

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

R

E

P

P

R

S

L

V

L

F

L

L

D

D

E
|
E

P

P

L

L

S

S

A

N

L

L

D

D

R

A

A

A

L

L

R

R

E

V

Q

Q

L

M

A

R

V

I

D

E

L

I

R

S

A

R

I

L

L

H

A

K

E

R

Q

L

G

G

T

R

T

T

A

M

L

I

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

V

E

E

A

A

M

M

T

T

V

L

A

A

D

D

E

K

V

I

G

V

V

V

M

L

E

D

A

A

G

G

R

R

L

V

A

A

R

Q

L

V

A

G

A

K

P

P

A

L

E

E

L

L

W

Y

A

H

D

Y

P

P

G

A

S

D

A

R

S

F

V

V

A

A

A

G

F

F

L

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ A20), V17 (≠ A25), G38 (= G46), C39 (≠ S47), G40 (= G48), K41 (= K49), S42 (= S50), T43 (≠ S51), R128 (≠ Y131), A132 (≠ T138), S134 (= S140), Q137 (= Q143)
binding tetrafluoroaluminate ion: S37 (= S45), G38 (= G46), K41 (= K49), Q81 (= Q89), S134 (= S140), G135 (= G141), G136 (= G142), E158 (= E164), H191 (= H197)
binding magnesium ion: S42 (= S50), Q81 (= Q89)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
42% identity, 60% coverage: 18:240/372 of query aligns to 10:234/371 of 3puvA

query
sites
3puvA

R

K

G

A

A
x
W

G

G

P

E

V

V

A
x
V

V

S

D

K

G

D

V

I

D

N

L

L

E

D

I

I

P

H

A

E

G

G

R

E

I

F

V

V

A

V

L

F

L

V

G

G

A

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

P

T

V

I

A

T

A

S

G

G

S

D

I

L

R

F

W

I

D

G

G

E

R

K

D

R

V

M

V

N

G

D

T

T

P

P

V

P

H

A

R

E

R

R

G

G

F

V

G

G

L

M

M

V

F

F

Q
|
Q

E

S

G

Y

Q

A

L

L

F

Y

P

P

F

H

R

L

D

S

V

V

G

A

G

E

N

N

V

M

A

S

Y

F

G

G

L

L

T

K

-

L

-

A

G

G

L

A

P

K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

A

V

R

N

R

Q

V

V

A

A

E

E

M

V

L

L

E

Q

L

L

V

A

G

H

L

L

P

L

G

D

Y
x
R

G

K

P

P

R

K

P

-

I

-

T

-

T
x
A

L

L

S
|
S

G

G

Q
|
Q

A

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

R

E

P

P

R

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

R

A

A

A

L

L

R

R

E

V

Q

Q

L

M

A

R

V

I

D

E

L

I

R

S

A

R

I

L

L

H

A

K

E

R

Q

L

G

G

T

R

T

T

A

M

L

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

M

M

T

T

V

L

A

A

D

D

E

K

V

I

G

V

V

V

M

L

E

D

A

A

G

G

R

R

L

V

A

A

R

Q

L

V

A

G

A

K

P

P

A

L

E

E

L

L

W

Y

A

H

D

Y

P

P

G

A

S

D

A

R

S

F

V

V

A

A

A

G

F

F

L

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ A20), V17 (≠ A25), G38 (= G46), C39 (≠ S47), G40 (= G48), K41 (= K49), S42 (= S50), T43 (≠ S51), R128 (≠ Y131), A132 (≠ T138), S134 (= S140), Q137 (= Q143)
binding magnesium ion: S42 (= S50), Q81 (= Q89)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
42% identity, 60% coverage: 18:240/372 of query aligns to 10:234/374 of 2awnB

query
sites
2awnB

R

K

G

A

A
x
W

G

G

P

E

V

V

A
x
V

V

S

D

K

G

D

V

I

D

N

L

L

E

D

I

I

P

H

A

E

G

G

R

E

I

F

V

V

A

V

L

F

L

V

G

G

A

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

P

T

V

I

A

T

A

S

G

G

S

D

I

L

R

F

W

I

D

G

G

E

R

K

D

R

V

M

V

N

G

D

T

T

P

P

V

P

H

A

R

E

R

R

G

G

F

V

G

G

L

M

M

V

F

F

Q

Q

E

S

G

Y

Q

A

L

L

F

Y

P

P

F

H

R

L

D

S

V

V

G

A

G

E

N

N

V

M

A

S

Y

F

G

G

L

L

T

K

-

L

-

A

G

G

L

A

P

K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

A

V

R

N

R

Q

V

V

A

A

E

E

M

V

L

L

E

Q

L

L

V

A

G

H

L

L

P

L

G

D

Y

R

G

K

P

P

R

K

P

-

I

-

T

-

T

A

L

L

S

S

G

G

Q

Q

A

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

R

E

P

P

R

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

R

A

A

A

L

L

R

R

E

V

Q

Q

L

M

A

R

V

I

D

E

L

I

R

S

A

R

I

L

L

H

A

K

E

R

Q

L

G

G

T

R

T

T

A

M

L

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

M

M

T

T

V

L

A

A

D

D

E

K

V

I

G

V

V

V

M

L

E

D

A

A

G

G

R

R

L

V

A

A

R

Q

L

V

A

G

A

K

P

P

A

L

E

E

L

L

W

Y

A

H

D

Y

P

P

G

A

S

D

A

R

S

F

V

V

A

A

A

G

F

F

L

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ A20), V17 (≠ A25), S37 (= S45), G38 (= G46), G40 (= G48), K41 (= K49), S42 (= S50), T43 (≠ S51)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
42% identity, 60% coverage: 18:240/372 of query aligns to 8:232/367 of 1q12A

query
sites
1q12A

R

K

G

A

A
x
W

G

G

P

E

V

V

A

V

V

S

D

K

G

D

V

I

D

N

L

L

E

D

I

I

P

H

A

E

G

G

R

E

I

F

V

V

A

V

L

F

L

V

G

G

A

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

P

T

V

I

A

T

A

S

G

G

S

D

I

L

R

F

W

I

D

G

G

E

R

K

D

R

V

M

V

N

G

D

T

T

P

P

V

P

H

A

R

E

R

R

G

G

F

V

G

G

L

M

M

V

F

F

Q

Q

E

S

G

Y

Q

A

L

L

F

Y

P

P

F

H

R

L

D

S

V

V

G

A

G

E

N

N

V

M

A

S

Y

F

G

G

L

L

T

K

-

L

-

A

G

G

L

A

P

K

R

K

A

E

-

V

-

I

-

N

E

Q

R

R

A

V

R

N

R

Q

V

V

A

A

E

E

M

V

L

L

E

Q

L

L

V

A

G

H

L

L

P

L

G

D

Y
x
R

G

K

P

P

R

K

P

-

I

-

T

-

T
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

A

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

R

E

P

P

R

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

R

A

A

A

L

L

R

R

E

V

Q

Q

L

M

A

R

V

I

D

E

L

I

R

S

A

R

I

L

L

H

A

K

E

R

Q

L

G

G

T

R

T

T

A

M

L

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

M

M

T

T

V

L

A

A

D

D

E

K

V

I

G

V

V

V

M

L

E

D

A

A

G

G

R

R

L

V

A

A

R

Q

L

V

A

G

A

K

P

P

A

L

E

E

L

L

W

Y

A

H

D

Y

P

P

G

A

S

D

A

R

S

F

V

V

A

A

A

G

F

F

L

I

G

G

binding adenosine-5'-triphosphate: W10 (≠ A20), S35 (= S45), G36 (= G46), C37 (≠ S47), G38 (= G48), K39 (= K49), S40 (= S50), T41 (≠ S51), R126 (≠ Y131), A130 (≠ T138), S132 (= S140), G134 (= G142), Q135 (= Q143)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
42% identity, 60% coverage: 18:240/372 of query aligns to 11:235/369 of P19566

query
sites
P19566

R

K

G

A

A

W

G

G

P

D

V

V

A

V

V

S

D

K

G

D

V

I

D

N

L

L

E

D

I

I

P

H

A

D

G

G

R

E

I

F

V

V

A

V

L

F

L

V

G

G

A

P

S

S

G

G

S

C

G

G

K

K

S

S

S

T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

P

T

V

I

A

T

A

S

G

G

S

D

I

L

R

F

W

I

D

G

G

E

R

T

D

R

V

M

V

N

G

D

T

I

P

P

V

P

H

A

R

E

R

R

G

G

F

V

G

G

L

M

M

V

F

F

Q

Q

E

S

G

Y

Q

A

L
|
L

F

Y

P

P

F

H

R

L

D

S

V

V

G

A

G

E

N

N

V

M

A

S

Y

F

G

G

L

L

T

K

-

L

-

A

G

G

L

A

P

K

R

K

A

E

-

V

-

M

-

N

E

Q

R

R

A

V

R

N

R

Q

V

V

A

A

E

E

M

V

L

L

E

Q

L

L

V

A

G

H

L

L

P

L

G

E

Y

R

G

K

P

P

R

K

P

-

I

-

T

-

T

A

L

L

S

S

G

G

Q

Q

A

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

R

E

P

P

R

R

L

V

L

F

L

L

D

D

E

E

P
|
P

L

L

S

S

A

N

L

L

D
|
D

R

A

A

A

L

L

R

R

E

V

Q

Q

L

M

A

R

V

I

D

E

L

I

R

S

A

R

I

L

L

H

A

K

E

R

Q

L

G

G

T

R

T

T

A

M

L

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

M

M

T

T

V

L

A

A

D

D

E

K

V

I

G

V

V

V

M

L

E

D

A

A

G

G

R

R

L

V

A

A

R

Q

L

V

A

G

A

K

P

P

A

L

E

E

L

L

W

Y

A

H

D

Y

P

P

G

A

S

D

A

R

S

F

V

V

A

A

A

G

F

F

L

I

G

G

L86 (= L93) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P165) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D170) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

2d62A Crystal structure of multiple sugar binding transport atp-binding protein
41% identity, 60% coverage: 21:244/372 of query aligns to 17:248/375 of 2d62A

query
sites
2d62A

G

G

P

D

V

V

V

T

A

A

V

V

D

K

G

D

V

L

D

S

L

L

E

E

I

I

P

K

A

D

G

G

R

E

I

F

V

L

A

V

L

L

G

G

A

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

S
x
T

L

T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

P

E

V

P

A

T

A

R

G

G

S

Q

I

I

R

Y

W

I

D

E

G

D

R

N

D

L

V

V

-

A

-

D

-

P

-

E

-

K

-

G

V

V

G

F

T

V

P

P

V

P

H

K

R

E

R

R

G

D

F

V

G

A

L

M

M

V

F

F

Q

Q

E

S

G

Y

Q

A

L

L

F

Y

P

P

F

H

R

M

D

T

V

V

G

Y

G

D

N

N

V

I

A

A

Y

F

-

P

-

L

G

K

L

L

T

R

G

K

L

V

P

P

R

K

A

Q

E

E

R

I

A

D

R

K

R

R

V

V

A

R

E

E

M

V

L

A

E

E

L

M

V

L

G

G

L

L

P

T

G

E

Y

L

G

L

P

N

R

R

P

K

I

P

T

R

T

E

L

L

S

S

G

G

Q

Q

A

R

Q

Q

R

R

V

V

A

A

L

L

A

G

R

R

A

A

L

I

A

I

P

R

R

R

P

P

R

K

L

V

L

F

L

L

L

M

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

R

A

A

K

L

L

R

R

E

V

Q

K

L

M

A

R

V

A

D

E

L

L

R

K

A

K

I

L

L

Q

A

R

E

Q

Q

L

G

G

T

V

T

T

A

T

L

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

M

M

T

T

V

M

A

G

D

D

E

R

V

I

G

A

V

V

M

M

E

N

A

K

G

G

R

E

L

L

A

Q

R

Q

L

V

A

G

A

T

P

P

A

D

E

E

L

V

W

Y

A

Y

D

K

P

P

G

V

S

N

A

T

S

F

V

V

A

A

A

G

F

F

L

I

G

G

F

S

G

P

P

P

I

M

binding pyrophosphate 2-: G42 (= G46), C43 (≠ S47), G44 (= G48), K45 (= K49), T46 (≠ S50), T47 (≠ S51)

1g291 Malk (see paper)
40% identity, 60% coverage: 21:244/372 of query aligns to 14:245/372 of 1g291

query
sites
1g291

G

G

P

E

V

V

V

T

A

A

V

V

D

R

G

E

V

M

D

S

L

L

E

E

I

V

P

K

A

D

G

G

R

E

I

F

V

M

A

I

L

L

G

G

A

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
x
T

L

T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

P

E

V

P

A

S

A

R

G

G

S

Q

I

I

R

Y

W

I

D

G

G

D

R

K

D

L

V

V

-

A

-
x
D

-

P

-
x
E

-
x
K

-

G

V

I

G

F

T

V

P

P

V

P

H
x
K

R
x
D

R

R

G

D

F

I

G

A

L

M

M

V

F

F

Q

Q

E

S

G

Y

Q

A

L

L

F

Y

P

P

F

H

R

M

D

T

V

V

G

Y

G

D

N

N

V

I

A

A

Y

F

-

P

-

L

G

K

L

L

T

R

G

K

L

V

P

P

R

R

A

Q

E

E

R

I

A

D

R

Q

R

R

V

V

A

R

E

E

M

V

L

A

E

E

L

L

V

L

G

G

L

L

P

T

G

E

Y

L

G

L

P

N

R

R

P

K

I

P

T

R

T

E

L

L

S

S

G

G

Q

Q

A

R

Q

Q

R

R

V

V

A

A

L

L

A

G

R

R

A

A

L

I

A

V

P

R

R

K

P

P

R

Q

L

V

L

F

L

L

L

M

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

R

A

A

K

L

L

R

R

E

V

Q

R

L

M

A

R

V

A

D

E

L

L

R

K

A

K

I

L

L

Q

A

R

E

Q

Q

L

G

G

T

V

T

T

A

T

L

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

M

M

T

T

V

M

A

G

D

D

E

R

V

I

G

A

V

V

M

M

E

N

A

R

G

G

R

V

L

L

A

Q

R

Q

L

V

A

G

A

S

P

P

A

D

E

E

L

V

W

Y

A

D

D

K

P

P

G

A

S

N

A

T

S

F

V

V

A

A

A

G

F

F

L

I

G

G

F

S

G

P

P

P

I

M

binding magnesium ion: D69 (vs. gap), E71 (vs. gap), K72 (vs. gap), K79 (≠ H80), D80 (≠ R81)
binding pyrophosphate 2-: S38 (= S45), G39 (= G46), C40 (≠ S47), G41 (= G48), K42 (= K49), T43 (≠ S50), T44 (≠ S51)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 359

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
39% identity, 59% coverage: 21:240/372 of query aligns to 17:230/353 of 1vciA

query
sites
1vciA

G

G

P

N

V
x
F

V

T

A

A

V

V

D

N

G

K

V

L

D

N

L

L

E

T

I

I

P

K

A

D

G

G

R

E

I

F

V

L

A

V

L

L

G

G

A

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

S
x
T

L

T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

P

E

V

P

A

T

A

E

G

G

S

R

I

I

R

Y

W

F

D

G

G

D

R

R

D

D

V

V

V

T

G

Y

T

L

P

P

V

P

H

K

R

D

R

R

G

N

F

I

G

S

L

M

M

V

F

F

Q

Q

E

H

G

M

Q

T

L

V

F

Y

P

E

F

-

R

-

D

-

V

-

G

-

N

N

V

I

A

A

Y

F

G

P

L

L

T

K

G

K

L

F

P

P

R

K

A

D

E

E

R

I

A

D

R

K

R

R

V

V

-

R

-

W

-

A

A

A

E

E

M

L

L

L

E

Q

L

I

V

E

G

E

L

L

P

L

G

N

Y

R

G

Y

P

P

R

-

P

-

I

-

T

A

T

Q

L

L

S

S

G

G

Q

Q

A

R

Q

Q

R

R

V

V

A

A

L

V

A

A

R

R

A

A

L

I

A

V

P

V

R

E

P

P

R

D

L

V

L

L

L

M

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

R

A

A

K

L

L

R

R

E

V

Q

A

L

M

A

R

V

A

D

E

L

I

R

K

A

K

I

L

L

Q

A

Q

E

K

Q

L

G

K

T

V

T

T

A

T

L

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

M

M

T

T

V

M

A

G

D

D

E

R

V

I

G

A

V

V

M

M

E

N

A

R

G

G

R

Q

L

L

A

L

R

Q

L

I

A

G

A

S

P

P

A

T

E

E

L

V

W

Y

A

L

D

R

P

P

G

N

S

S

A

V

S

F

V

V

A

A

A

T

F

F

L

I

G

G

binding adenosine-5'-triphosphate: F19 (≠ V23), S41 (= S45), G42 (= G46), G44 (= G48), K45 (= K49), T46 (≠ S50), T47 (≠ S51)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 16

8hprD Lpqy-sugabc in state 4 (see paper)
38% identity, 63% coverage: 5:240/372 of query aligns to 3:235/362 of 8hprD

query
sites
8hprD

L

I

T

V

I

L

D

D

G

R

L

V

R

T

V

K

V

S

Y
|
Y

P

P

G

D

G

V

R

R

G

A

A

A

G

-

P

-

V

-

A

-

V

V

D

K

G

E

V

F

D

S

L

M

E

T

I

I

P

A

A

D

G

G

R

E

I

F

V

I

A

I

L

L

L

V

G

G

A

P

S
|
S

G

G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

T

L

L

R

N

A

M

V

I

A

A

G

G

L

L

E

E

P

E

V

I

A

T

A

S

G

G

S

E

I

L

R

R

W

I

D

G

G

G

R

E

D

R

V

V

V

N

G

E

T

K

P

A

V

P

H

K

R

D

R

R

G

D

F

I

G

A

L

M

M

V

F

F

Q
|
Q

E

S

G

Y

Q

A

L

L

F

Y

P

P

F

H

R

M

D

T

V

V

G

R

G

Q

N

N

V

I

A

A

Y

F

G

P

L

L

T

T

-

L

-

A

G

K

L

V

P

P

R

K

A

A

E

E

R

I

A

A

R

A

R

K

V

V

A

E

E

E

M

T

L

A

E

K

L

I

V

L

G

D

L

L

P

S

G

E

Y

L

G

L

P

D

R
|
R

P

K

I

P

T

G

T
x
Q

L

L

S
|
S

G
|
G

Q

Q

A

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

P

R

R

S

P

P

R

K

L

A

L

F

L

L

L

M

D

D

E
x
Q

P

P

L

L

S

S

A

N

L

L

D

D

R

A

A

K

L

L

R

R

E

V

Q

Q

L

M

A

R

V

A

D

E

L

I

R

S

A

R

I

L

L

Q

A

D

E

R

Q

L

G

G

T

T

A

T

L

V

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

T

E

E

A

A

M

M

T

T

V

L

A

G

D

D

E

R

V

V

G

V

V

V

M

M

E

L

A

A

G

G

R

E

L

V

A

Q

R

Q

L

I

A

G

A

T

P

P

A

D

E

E

L

L

W

Y

A

S

D

S

P

P

G

A

S

N

A

L

S

F

V

V

A

A

A

G

F

F

L

I

G

G

binding adenosine-5'-triphosphate: Y12 (= Y14), S38 (= S45), C40 (≠ S47), G41 (= G48), K42 (= K49), S43 (= S50), T44 (≠ S51), Q82 (= Q89), R129 (= R134), Q133 (≠ T138), S135 (= S140), G136 (= G141), G137 (= G142), Q159 (≠ E164), H192 (= H197)
binding magnesium ion: S43 (= S50), Q82 (= Q89)

8hplC Lpqy-sugabc in state 1 (see paper)
40% identity, 59% coverage: 22:240/372 of query aligns to 13:233/384 of 8hplC

query
sites
8hplC

P

P

V

R

V

A

A

A

V

V

D

K

G

E

V

F

D

S

L

M

E

T

I

I

P

A

A

D

G

G

R

E

I

F

V

I

A

I

L

L

L

V

G

G

A

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

T

L

L

R

N

A

M

V

I

A

A

G

G

L

L

E

E

P

E

V

I

A

T

A

S

G

G

S

E

I

L

R

R

W

I

D

G

G

G

R

E

D

R

V

V

V

N

G

E

T

K

P

A

V

P

H

K

R

D

R

R

G

D

F

I

G

A

L

M

M

V

F

F

Q

Q

E

S

G

Y

Q

A

L

L

F

Y

P

P

F

H

R

M

D

T

V

V

G

R

G

Q

N

N

V

I

A

A

Y

F

G

P

L

L

T

T

-

L

-

A

G

K

L

V

P

P

R

K

A

A

E

E

R

I

A

A

R

A

R

K

V

V

A

E

E

E

M

T

L

A

E

K

L

I

V

L

G

D

L

L

P

S

G

E

Y

L

G

L

P

D

R

R

P

K

I

P

T

G

T

Q

L

L

S

S

G

G

Q

Q

A

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

P

R

R

S

P

P

R

K

L

A

L

F

L

L

L

M

D

D

E

Q

P

P

L

L

S

S

A

N

L

L

D

D

R

A

A

K

L

L

R

R

E

V

Q

Q

L

M

A

R

V

A

D

E

L

I

R

S

A

R

I

L

L

Q

A

D

E

R

Q

L

G

G

T

T

A

T

L

V

Y

Y

V

V

T

T

H

H

D

D

Q

Q

D

T

E

E

A

A

M

M

T

T

V

L

A

G

D

D

E

R

V

V

G

V

V

V

M

M

E

L

A

A

G

G

R

E

L

V

A

Q

R

Q

L

I

A

G

A

T

P

P

A

D

E

E

L

L

W

Y

A

S

D

S

P

P

G

A

S

N

A

L

S

F

V

V

A

A

A

G

F

F

L

I

G

G

binding adenosine-5'-triphosphate: G37 (= G46), C38 (≠ S47), G39 (= G48), K40 (= K49), S41 (= S50), T42 (≠ S51)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

8hprC Lpqy-sugabc in state 4 (see paper)
38% identity, 63% coverage: 5:240/372 of query aligns to 3:235/363 of 8hprC

query
sites
8hprC

L

I

T

V

I

L

D

D

G

R

L

V

R

T

V

K

V

S

Y
|
Y

P

P

G

D

G

V

R

R

G

A

A

A

G

-

P

-

V

-

A

-

V

V

D

K

G

E

V

F

D

S

L

M

E

T

I

I

P

A

A

D

G

G

R

E

I

F

V

I

A

I

L

L

L

V

G

G

A

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

S
|
S

S

T

L

T

L

L

R

N

A

M

V

I

A

A

G

G

L

L

E

E

P

E

V

I

A

T

A

S

G

G

S

E

I

L

R

R

W

I

D

G

G

G

R

E

D

R

V

V

V

N

G

E

T

K

P

A

V

P

H

K

R

D

R

R

G

D

F

I

G

A

L

M

M

V

F

F

Q
|
Q

E

S

G

Y

Q

A

L

L

F

Y

P

P

F

H

R

M

D

T

V

V

G

R

G

Q

N

N

V

I

A

A

Y

F

G

P

L

L

T

T

-

L

-

A

G

K

L

V

P

P

R

K

A

A

E

E

R

I

A

A

R

A

R

K

V

V

A

E

E

E

M

T

L

A

E

K

L

I

V

L

G

D

L

L

P

S

G

E

Y

L

G

L

P

D

R

R

P

K

I

P

T

G

T
x
Q

L

L

S

S

G
|
G

Q
|
Q

A

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

P

R

R

S

P

P

R

K

L

A

L

F

L

L

L

M

D

D

E

Q

P

P

L

L

S

S

A

N

L

L

D

D

R

A

A

K

L

L

R

R

E

V

Q

Q

L

M

A

R

V

A

D

E

L

I

R

S

A

R

I

L

L

Q

A

D

E

R

Q

L

G

G

T

T

A

T

L

V

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

T

E

E

A

A

M

M

T

T

V

L

A

G

D

D

E

R

V

V

G

V

V

V

M

M

E

L

A

A

G

G

R

E

L

V

A

Q

R

Q

L

I

A

G

A

T

P

P

A

D

E

E

L

L

W

Y

A

S

D

S

P

P

G

A

S

N

A

L

S

F

V

V

A

A

A

G

F

F

L

I

G

G

binding adenosine-5'-triphosphate: Y12 (= Y14), S38 (= S45), G39 (= G46), G41 (= G48), K42 (= K49), S43 (= S50), Q82 (= Q89), Q133 (≠ T138), G136 (= G141), G137 (= G142), Q138 (= Q143), H192 (= H197)
binding magnesium ion: S43 (= S50), Q82 (= Q89)

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
37% identity, 63% coverage: 5:240/372 of query aligns to 4:236/393 of P9WQI3

query
sites
P9WQI3

L

I

T

V

I

L

D

D

G

H

L

V

R

N

V

K

V

S

Y

Y

P

P

G

D

G

G

R

H

G

-

A

-

G

-

P

-

V

-

V

T

A

A

V

V

D

R

G

D

V

L

D

N

L

L

E

T

I

I

P

A

A

D

G

G

R

E

I

F

V

L

A

I

L

L

L

V

G

G

A

P

S

S

G

G

S

C

G

G

K

K

S

T

L

T

L

L

R

N

A

M

V

I

A

A

G

G

L

L

E

E

P

D

V

I

A

S

G

G

S

E

I

L

R

R

W

I

D

A

G

G

R

E

D

R

V

V

V

N

G

E

T

K

P

A

V

P

H

K

R

D

R

R

G

D

F

I

G

A

L

M

M

V

F

F

Q

Q

E

S

G

Y

Q

A

L

L

F

Y

P

P

F

H

R

M

D

T

V

V

G

R

G

Q

N

N

V

I

A

A

Y

F

G

P

L

L

T

T

-

L

-

A

G

K

L

M

P

R

R

K

A

A

E

D

R

I

A

A

R

Q

R

K

V

V

A

S

E

E

M

T

L

A

E

K

L

I

V

L

G

D

L

L

P

T

G

N

Y

L

G

L

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D

R

R

P

K

I

P

T

S

T

Q

L

L

S

S

G

G

Q

Q

A

R

Q

Q

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R

V

V

A

A

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M

A

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R

A

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A

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R

R

H

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P

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K

L

A

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F

L

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D

D

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E

P

P

L

L

S

S

A

N

L

L

D

D

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A

A

K

L

L

R

R

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V

Q

Q

L

M

A

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D

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A

A

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I

L

L

Q

A

R

E

R

Q

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G

G

T

T

A

T

L

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Y

Y

V

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T

T

H
|
H

D

D

Q

Q

D

T

E

E

A

A

M

M

T

T

V

L

A

G

D

D

E

R

V

V

G

V

V

V

M

M

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Y

A

G

G

G

R

I

L

A

A

Q

R

Q

L

I

A

G

A

T

P

P

A

E

E

E

L

L

W

Y

A

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D

R

P

P

G

A

S

N

A

L

S

F

V

V

A

A

A

G

F

F

L

I

G

G

H193 (= H197) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

3fvqB Crystal structure of the nucleotide binding domain fbpc complexed with atp (see paper)
43% identity, 59% coverage: 26:245/372 of query aligns to 19:244/350 of 3fvqB

query
sites
3fvqB

V

L

D

N

G

D

V

I

D

S

L

L

E

S

I

L

P

D

A

P

G

G

R

E

I

I

V

L

A

F

L

I

G

G

A

A

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
x
T

L

L

R

R

A

C

V

L

A

A

G

G

L

F

E

E

P

Q

V

P

A

D

A

S

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G

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I

I

R

S

W

L

D

S

G

G

R

K

D

T

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I

V

F

G

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T

K

-

N

-

T

-

N

-

L

P

P

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V

H

R

R

E

R

R

G

R

F

L

G

G

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Y

M

L

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Q
|
Q

E

E

G

G

Q

V

L

L

F

F

P

P

F

H

R

L

D

T

V

V

G

Y

G

R

N

N

V

I

A

A

Y

Y

G

G

L

L

-

G

T

N

G

G

L

K

P

G

R

R

-

T

A

A

E

Q

R

E

A

R

R

Q

R

R

V

I

A

E

E

A

M

M

L

L

E

E

L

L

V

T

G

G

L

I

P

S

G

E

Y

L

G

A

P

G

R
|
R

P

Y

I

P

T

H

T
x
E

L

L

S
|
S

G

G

G
|
G

Q
|
Q

A

Q

Q

Q

R

R

V

A

A

A

L

L

A

A

R

R

A

A

L

L

A

A

P

P

R

D

P

P

R

E

L

L

L

I

L

L

D

D

E

E

P

P

L

F

S

S

A

A

L

L

D

D

R

E

A

Q

L

L

R

R

E

R

Q

Q

L

I

A

R

V

E

D

D

L

M

R

I

A

A

I

A

L

L

A

R

E

A

Q

N

G

G

T

K

T

S

A

A

L

V

Y

F

V

V

T

S

H

H

D

D

Q

R

D

E

E

E

A

A

M

L

T

Q

V

Y

A

A

D

D

E

R

V

I

G

A

V

V

M

M

E

K

A

Q

G

G

R

R

L

I

A

L

R

Q

L

T

A

A

A

S

P

P

A

H

E

E

L

L

W

Y

A

R

D

Q

P

P

G

A

S

D

A

L

S

D

V

A

A

A

A

L

F

F

L

I

G

G

F

E

G

G

P

I

I

V

L

F

binding adenosine-5'-triphosphate: S38 (= S45), G39 (= G46), C40 (≠ S47), G41 (= G48), K42 (= K49), T43 (≠ S50), T44 (≠ S51), R133 (= R134), E137 (≠ T138), S139 (= S140), G141 (= G142), Q142 (= Q143)
binding calcium ion: T43 (≠ S50), Q86 (= Q89)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 13, 14, 16, 18

2awnC Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
39% identity, 59% coverage: 23:240/372 of query aligns to 8:204/344 of 2awnC

query
sites
2awnC

V

V

V

T

A
x
V

V

S

D

K

G

D

V

I

D

N

L

L

E

D

I

I

P

H

A

E

G

G

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E

I

F

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V

A

V

L

F

L

V

G

G

A

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

-

P

-

V

-

A

-

G

-

S

-

I

-

R

-

W

-

D

-

G

-

R

-

D

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V

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G

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P

-

V

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H

-

R

-

G

G

F

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G

G

L

M

M

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F

F

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Q

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Q

A

L

L

F

Y

P

P

F

H

R

L

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S

V

V

G

A

G

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N

N

V

M

A

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Y

F

G

G

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L

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K

-

L

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A

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G

L

A

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A

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L

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L

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I

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A

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G

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Q

A

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Q

Q

R

R

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V

A

A

L

I

A

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T

L

L

A

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A

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P

R

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

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A

A

A

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L

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R

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Q

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A

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A

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L

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A

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Y

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T

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H

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D

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Q

D

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A

A

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M

T

T

V

L

A

A

D

D

E

K

V

I

G

V

V

V

M

L

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D

A

A

G

G

R

R

L

V

A

A

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V