SitesBLAST

C26 (≠ V27) mutation to A: Lower ATPase activity and transport efficiency.
F27 (= F28) mutation to L: Lower ATPase activity and transport efficiency.
F45 (= F50) mutation to L: Lower ATPase activity and transport efficiency.
C54 (= C59) mutation to T: Loss of ATPase activity and transport.
L60 (= L65) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L81) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ M140) mutation to M: Loss of ATPase activity and transport.
D172 (= D177) mutation to N: Loss of ATPase activity and transport.
C276 (≠ L280) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E301) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
43% identity, 92% coverage: 19:375/386 of query aligns to 3:353/358 of 8y5iA

query
sites
8y5iA

L

V

E

Q

L

L

R

A

D

G

V

I

V

R

K

K

V

C

F
|
F

S

D

N

-

R

-

G

G

K
|
K

D

E

V

V

Y

-

A

-

V

I

H

P

G

Q

V

L

S

D

L

L

D

T

I

I

A

N

P

N

G

G

E

E

F

F

V

L

T

T

L

L

G

G

P

P

S

S

G
|
G

C

C

G

G

K
|
K

T
|
T

T

V

L

L

R

R

M

L

I

I

A

A

G

G

F

L

E

E

D

T

T

V

T

D

S

S

G

G

Q

R

V

I

V

M

L

L

D

D

G

N

Q

E

N

D

M

I

V

T

S

H

L

V

P

P

P

A

N

E

K

N

R

R

P

Y

M

V

S

N

M

T

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

F

P

P

H

H

L

M

S

T

V

V

R

F

E

E

N

N

I

V

A

A

Y

F

G

G

L

L

K

R

L

M

R

Q

H

K

T

T

K

P

P

A

E

A

E

E

I

I

R

T

E

P

Q

R

V

V

E

M

I

E

A

A

L

L

T

R

S

M

M

V

N

Q

L

L

N

E

S

T

L

F

A

A

D

Q

R
|
R

A

K

P

P

N

H

E
x
Q

L

L

S
|
S

G

G

Q

Q

R

R

V

V

A

A

L

I

A

A

R

R

A

A

M

V

V

V

M

N

R

K

P

P

K

R

V

L

L

L

F

L

D

D

E

Q

P

S

L

L

S

S

N

A

L

L

D

D

A

Y

K

K

L

L

R

R

V

K

R

Q

M

M

R

Q

L

N

E

E

I

L

R

K

R

A

L

L

Q

Q

Q

R

R

K

M

L

G

G

I

I

T

T

S

F

I

V

Y

F

V

V

T

T

H

H

D

D

Q

Q

A

E

E

E

A

A

M

L

T

T

M

M

S

S

D

D

R

R

I

I

V

V

M

M

N

R

A

D

G

G

T

R

I

I

E

E

Q

Q

V

D

A

G

T

T

P

P

E

R

K

E

I

I

Y

Y

R

E

P

P

A

K

S

N

V

L

F

F

V

V

A

A

D

G

F

F

I

I

G

G

R

E

A

I

N

N

F

M

L

F

A

N

A

A

T

T

A

V

R

I

D

E

-

R

V

L

G

D

G

E

G

Q

R

R

C

V

S

R

A

A

R

N

V

V

L

E

G

G

A

R

D

E

L

C

Q

N

A

I

A

Y

C

V

H

N

E

F

G

A

V

V

S

E

A

P

G

G

S

Q

G

K

V

L

T

H

V

V

I

L

V

L

R

R

P

P

E

E

S

D

V

L

R

R

L

V

S

E

P

-

G

-

T

-

G

-

E

E

G

I

A

N

G

D

S

N

E

H

L

A

V

E

G

G

A

L

H

I

G

G

R

Y

V

V

L

R

S

E

S

R

V

N

F

Y

Y

K

G

G

D

M

H

T

V

L

E

E

Y

S

E

V

V

V

E

E

T

L

E

E

A

N

G

G

T

K

I

M

L

V

C

M

V

V

E

S

-

E

-

F

S

N

D

E

P

D

E

D

P

P

S

D

S

F

V

D

H

H

A

S

E

L

G

-

D

-

D

D

V

Q

M

K

V

M

A

A

V

I

E

N

A

W

S

V

R

E

A

S

W

W

binding adenosine-5'-triphosphate: F12 (= F28), K15 (= K33), G38 (= G58), K41 (= K61), T42 (= T62), T43 (= T63), R128 (= R148), Q132 (≠ E152), S134 (= S154)

8hplC Lpqy-sugabc in state 1 (see paper)
55% identity, 60% coverage: 37:267/386 of query aligns to 16:248/384 of 8hplC

query
sites
8hplC

A

A

V

V

H

K

G

E

V

F

S

S

L

M

D

T

I

I

A

A

P

D

G

G

E

E

F

F

V

I

T

I

L

L

L

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

T

L

L

R

N

M

M

I

I

A

A

G

G

F

L

E

E

D

E

T

I

T

T

S

S

G

G

Q

E

V

L

V

R

L

I

D

G

G

G

Q

E

N

R

M

V

V

N

S

E

L

K

P

A

P

P

N

K

K

D

R

R

P

D

M

I

S

A

M

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

M

S

T

V

V

R

R

E

Q

N

N

I

I

A

A

Y

F

G

P

L

L

K

T

L

L

R

A

H

K

T

V

K

P

P

K

E

A

E

E

I

I

R

A

E

A

Q

K

V

V

E

E

I

E

A

T

L

A

T

K

S

I

M

L

N

D

L

L

N

S

S

E

L

L

A

L

D

D

R

R

A

K

P

P

N

G

E

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

M

I

V

V

M

R

R

S

P

P

K

K

V

A

L

F

L

L

F

M

D

D

E

Q

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

K

L

L

R

R

V

V

R

Q

M

M

R

R

L

A

E

E

I

I

R

S

R

R

L

L

Q

Q

Q

D

R

R

M

L

G

G

I

T

T

T

S

T

I

V

Y

Y

V

V

T

T

H

H

D

D

Q

Q

A

T

E

E

A

A

M

M

T

T

M

L

S

G

D

D

R

R

I

V

V

V

M

M

N

L

A

A

G

G

T

E

I

V

E

Q

Q

Q

V

I

A

G

T

T

P

P

E

D

K

E

I

L

Y

Y

R

S

P

P

A

A

S

N

V

L

F

F

V

V

A

A

D

G

F

F

I

I

G

G

R

S

-

P

-

A

A

M

N

N

F

F

L

F

A

P

A

A

T

T

A

R

R

T

D

D

V

V

G

G

binding adenosine-5'-triphosphate: G37 (= G58), C38 (= C59), G39 (= G60), K40 (= K61), S41 (≠ T62), T42 (= T63)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

8hprC Lpqy-sugabc in state 4 (see paper)
55% identity, 60% coverage: 37:267/386 of query aligns to 18:250/363 of 8hprC

query
sites
8hprC

A

A

V

V

H

K

G

E

V

F

S

S

L

M

D

T

I

I

A

A

P

D

G

G

E

E

F

F

V

I

T

I

L

L

L

V

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
x
S

T

T

L

L

R

N

M

M

I

I

A

A

G

G

F

L

E

E

D

E

T

I

T

T

S

S

G

G

Q

E

V

L

V

R

L

I

D

G

G

G

Q

E

N

R

M

V

V

N

S

E

L

K

P

A

P

P

N

K

K

D

R

R

P

D

M

I

S

A

M

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

M

S

T

V

V

R

R

E

Q

N

N

I

I

A

A

Y

F

G

P

L

L

K

T

L

L

R

A

H

K

T

V

K

P

P

K

E

A

E

E

I

I

R

A

E

A

Q

K

V

V

E

E

I

E

A

T

L

A

T

K

S

I

M

L

N

D

L

L

N

S

S

E

L

L

A

L

D

D

R

R

A

K

P

P

N

G

E
x
Q

L

L

S

S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

M

I

V

V

M

R

R

S

P

P

K

K

V

A

L

F

L

L

F

M

D

D

E

Q

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

K

L

L

R

R

V

V

R

Q

M

M

R

R

L

A

E

E

I

I

R

S

R

R

L

L

Q

Q

Q

D

R

R

M

L

G

G

I

T

T

T

S

T

I

V

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

A

T

E

E

A

A

M

M

T

T

M

L

S

G

D

D

R

R

I

V

V

V

M

M

N

L

A

A

G

G

T

E

I

V

E

Q

Q

Q

V

I

A

G

T

T

P

P

E

D

K

E

I

L

Y

Y

R

S

P

P

A

A

S

N

V

L

F

F

V

V

A

A

D

G

F

F

I

I

G

G

R

S

-

P

-

A

A

M

N

N

F

F

L

F

A

P

A

A

T

T

A

R

R

T

D

D

V

V

G

G

binding adenosine-5'-triphosphate: S38 (= S57), G39 (= G58), G41 (= G60), K42 (= K61), S43 (≠ T62), Q82 (= Q101), Q133 (≠ E152), G136 (= G155), G137 (= G156), Q138 (= Q157), H192 (= H211)
binding magnesium ion: S43 (≠ T62), Q82 (= Q101)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

8hprD Lpqy-sugabc in state 4 (see paper)
55% identity, 60% coverage: 37:267/386 of query aligns to 18:250/362 of 8hprD

query
sites
8hprD

A

A

V

V

H

K

G

E

V

F

S

S

L

M

D

T

I

I

A

A

P

D

G

G

E

E

F

F

V

I

T

I

L

L

L

V

G

G

P

P

S
|
S

G

G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

T

L

L

R

N

M

M

I

I

A

A

G

G

F

L

E

E

D

E

T

I

T

T

S

S

G

G

Q

E

V

L

V

R

L

I

D

G

G

G

Q

E

N

R

M

V

V

N

S

E

L

K

P

A

P

P

N

K

K

D

R

R

P

D

M

I

S

A

M

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

M

S

T

V

V

R

R

E

Q

N

N

I

I

A

A

Y

F

G

P

L

L

K

T

L

L

R

A

H

K

T

V

K

P

P

K

E

A

E

E

I

I

R

A

E

A

Q

K

V

V

E

E

I

E

A

T

L

A

T

K

S

I

M

L

N

D

L

L

N

S

S

E

L

L

A

L

D

D

R
|
R

A

K

P

P

N

G

E
x
Q

L

L

S
|
S

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

M

I

V

V

M

R

R

S

P

P

K

K

V

A

L

F

L

L

F

M

D

D

E
x
Q

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

K

L

L

R

R

V

V

R

Q

M

M

R

R

L

A

E

E

I

I

R

S

R

R

L

L

Q

Q

Q

D

R

R

M

L

G

G

I

T

T

T

S

T

I

V

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

A

T

E

E

A

A

M

M

T

T

M

L

S

G

D

D

R

R

I

V

V

V

M

M

N

L

A

A

G

G

T

E

I

V

E

Q

Q

Q

V

I

A

G

T

T

P

P

E

D

K

E

I

L

Y

Y

R

S

P

P

A

A

S

N

V

L

F

F

V

V

A

A

D

G

F

F

I

I

G

G

R

S

-

P

-

A

A

M

N

N

F

F

L

F

A

P

A

A

T

T

A

R

R

T

D

D

V

V

G

G

binding adenosine-5'-triphosphate: S38 (= S57), C40 (= C59), G41 (= G60), K42 (= K61), S43 (≠ T62), T44 (= T63), Q82 (= Q101), R129 (= R148), Q133 (≠ E152), S135 (= S154), G136 (= G155), G137 (= G156), Q159 (≠ E178), H192 (= H211)
binding magnesium ion: S43 (≠ T62), Q82 (= Q101)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
44% identity, 88% coverage: 37:375/386 of query aligns to 19:346/393 of P9WQI3

query
sites
P9WQI3

A

A

V

V

H

R

G

D

V

L

S

N

L

L

D

T

I

I

A

A

P

D

G

G

E

E

F

F

V

L

T

I

L

L

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

T

L

L

R

N

M

M

I

I

A

A

G

G

F

L

E

E

D

D

T

I

T

S

S

S

G

G

Q

E

V

L

V

R

L

I

D

A

G

G

Q

E

N

R

M

V

V

N

S

E

L

K

P

A

P

P

N

K

K

D

R

R

P

D

M

I

S

A

M

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

M

S

T

V

V

R

R

E

Q

N

N

I

I

A

A

Y

F

G

P

L

L

K

T

L

L

R

A

H

K

T

M

K

R

P

K

E

A

E

D

I

I

R

A

E

Q

Q

K

V

V

E

S

I

E

A

T

L

A

T

K

S

I

M

L

N

D

L

L

N

T

S

N

L

L

A

L

D

D

R

R

A

K

P

P

N

S

E

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

M

I

V

V

M

R

R

H

P

P

K

K

V

A

L

F

L

L

F

M

D

D

E

E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

K

L

L

R

R

V

V

R

Q

M

M

R

R

L

G

E

E

I

I

R

A

R

Q

L

L

Q

Q

Q

R

R

R

M

L

G

G

I

T

T

T

S

T

I

V

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

A

T

E

E

A

A

M

M

T

T

M

L

S

G

D

D

R

R

I

V

V

V

M

M

N

Y

A

G

G

G

T

I

I

A

E

Q

Q

Q

V

I

A

G

T

T

P

P

E

E

K

E

I

L

Y

Y

R

E

R

R

P

P

A

A

S

N

V

L

F

F

V

V

A

A

D

G

F

F

I

I

G

G

R

S

-

P

-

A

A

M

N

N

F

F

L

F

A

P

A

A

T

R

A

L

R

T

D

A

V

I

G

G

G

L

G

T

R

L

C

P

S

F

A

G

R

E

V

V

-

T

L

L

G

A

A

P

D

E

L

V

Q

Q

A

G

-

V

-

I

A

A

C

A

H

H

E

-

G

-

V

-

S

P

A

K

G

P

S

E

G

N

V

V

T

I

V

V

I

G

V

V

R

R

P

P

E

E

S

H

V

I

R

Q

L

-

S

-

P

-

G

-

T

-

G

-

E

-

G

-

A

-

G

-

G

D

S

A

E

A

L

L

V

I

G

D

A

A

H

Y

G

Q

R

R

V

I

L

R

S

A

S

L

V

T

F

F

Y

-

G

-

D

-

H

Q

V

V

E

K

Y

V

E

N

V

L

E

V

T

E

E

S

A

L

G

G

T

A

I

D

L

K

C

Y

V

L

E

Y

S

F

D

T

P

T

E

E

P

S

S

P

S

A

V

V

H

H

A

S

E

V

G

Q

D

L

D

D

V

E

M

L

V

A

A

E

V

V

E

E

A

G

S

E

R

S

A

A

W

L

H193 (= H211) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
53% identity, 62% coverage: 19:259/386 of query aligns to 4:242/369 of P19566

query
sites
P19566

L

V

E

Q

L

L

R

R

D

N

V

V

V

T

K

K

V

A

F

W

S

G

N

-

R

-

G

-

K

-

D

D

V

V

Y

V

A

V

V

S

H

K

G

D

V

I

S

N

L

L

D

D

I

I

A

H

P

D

G

G

E

E

F

F

V

V

T

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

T

L

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

D

T

T

I

T

T

S

S

G

G

Q

D

V

L

V

F

L

I

D

G

G

E

Q

T

N

R

M

M

V

N

S

D

L

I

P

P

N

A

K

E

R

R

P

G

M

V

S

G

M

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L
|
L

F

Y

P

P

H

H

L

L

S

S

V

V

R

A

E

E

N

N

I

M

A

S

Y

F

G

G

L

L

K

K

L

L

R

A

H

G

T

A

K

K

P

K

E

E

E

V

I

M

R

N

E

Q

Q

R

V

V

E

N

I

Q

A

V

L

A

T

E

S

V

M

L

N

Q

L

L

N

A

S

H

L

L

A

L

D

E

R

R

A

K

P

P

N

K

E

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

M

L

V

V

M

A

R

E

P

P

K

R

V

V

L

F

L

L

F

L

D

D

E

E

P
|
P

L

L

S

S

N

N

L

L

D
|
D

A

A

K

A

L

L

R

R

V

V

R

Q

M

M

R

R

L

I

E

E

I

I

R

S

R

R

L

L

Q

H

Q

K

R

R

M

L

G

G

I

R

T

T

S

M

I

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

A

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

N

D

A

A

G

G

T

R

I

V

E

A

Q

Q

V

V

A

G

T

K

P

P

E

L

K

E

I

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

V

R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

R

K

A

M

N

N

F

F

L

L

L86 (= L105) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P179) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D184) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
52% identity, 64% coverage: 19:265/386 of query aligns to 3:251/374 of 2awnB

query
sites
2awnB

L

V

E

Q

L

L

R

Q

D

N

V

V

V

T

K

K

V

A

F
x
W

S

G

N

-

R

-

G

-

K

-

D

E

V

V

Y

V

A
x
V

V

S

H

K

G

D

V

I

S

N

L

L

D

D

I

I

A

H

P

E

G

G

E

E

F

F

V

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

D

T

T

I

T

T

S

S

G

G

Q

D

V

L

V

F

L

I

D

G

G

E

Q

K

N

R

M

M

V

N

S

D

L

T

P

P

N

A

K

E

R

R

P

G

M

V

S

G

M

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

R

A

E

E

N

N

I

M

A

S

Y

F

G

G

L

L

K

K

L

L

R

A

H

G

T

A

K

K

P

K

E

E

E

V

I

I

R

N

E

Q

Q

R

V

V

E

N

I

Q

A

V

L

A

T

E

S

V

M

L

N

Q

L

L

N

A

S

H

L

L

A

L

D

D

R

R

A

K

P

P

N

K

E

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

M

L

V

V

M

A

R

E

P

P

K

S

V

V

L

F

L

L

F

L

D

D

E

E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

V

V

R

Q

M

M

R

R

L

I

E

E

I

I

R

S

R

R

L

L

Q

H

Q

K

R

R

M

L

G

G

I

R

T

T

S

M

I

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

A

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

N

D

A

A

G

G

T

R

I

V

E

A

Q

Q

V

V

A

G

T

K

P

P

E

L

K

E

I

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

V

R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

R

K

A

M

N

N

F

F

L

L

-

P

-

V

-

K

-

V

A

T

A

A

T

T

A

A

R

I

D

D

binding adenosine-5'-diphosphate: W12 (≠ F28), V17 (≠ A37), S37 (= S57), G38 (= G58), G40 (= G60), K41 (= K61), S42 (≠ T62), T43 (= T63)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
52% identity, 64% coverage: 19:265/386 of query aligns to 4:252/371 of P68187

query
sites
P68187

L

V

E

Q

L

L

R

Q

D

N

V

V

V

T

K

K

V

A

F

W

S

G

N

-

R

-

G

-

K

-

D

E

V

V

Y

V

A

V

V

S

H

K

G

D

V

I

S

N

L

L

D

D

I

I

A

H

P

E

G

G

E

E

F

F

V

V

T

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

T

L

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

D

T

T

I

T

T

S

S

G

G

Q

D

V

L

V

F

L

I

D

G

G

E

Q

K

N

R

M

M

V

N

S

D

L

T

P

P

N

A

K

E

R

R

P

G

M

V

S

G

M

M

V

V

F

F

Q

Q

S

S

Y

Y

A
|
A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

R

A

E

E

N

N

I

M

A

S

Y

F

G

G

L

L

K

K

L

L

R

A

H

G

T

A

K
|
K

P

K

E

E

E

V

I

I

R

N

E

Q

Q

R

V
|
V

E

N

I

Q

A
x
V

L

A

T
x
E

S

V

M

L

N

Q

L

L

N
x
A

S

H

L

L

A

L

D

D

R

R

A

K

P

P

N

K

E

A

L

L

S

S

G

G

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

M

L

V

V

M

A

R

E

P

P

K

S

V

V

L

F

L

L

F

L

D
|
D

E

E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

V

V

R

Q

M

M

R

R

L

I

E

E

I

I

R

S

R

R

L

L

Q

H

Q

K

R

R

M

L

G

G

I

R

T

T

S

M

I

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

A

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

N

D

A

A

G

G

T

R

I

V

E

A

Q

Q

V

V

A

G

T

K

P

P

E

L

K

E

I

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

V
x
R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

R

K

A

M

N

N

F
|
F

L

L

-

P

-

V

-

K

-

V

A

T

A

A

T

T

A

A

R

I

D

D

A85 (= A104) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (= K125) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V133) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A136) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ T138) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ N143) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G156) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D177) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ V247) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
F241 (= F258) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
52% identity, 64% coverage: 19:265/386 of query aligns to 3:251/371 of 3puyA

query
sites
3puyA

L

V

E

Q

L

L

R

Q

D

N

V

V

V

T

K

K

V

A

F
x
W

S

G

N

-

R

-

G

-

K

-

D

E

V

V

Y

V

A

V

V

S

H

K

G

D

V

I

S

N

L

L

D

D

I

I

A

H

P

E

G

G

E

E

F

F

V

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

D

T

T

I

T

T

S

S

G

G

Q

D

V

L

V

F

L

I

D

G

G

E

Q

K

N

R

M

M

V

N

S

D

L

T

P

P

N

A

K

E

R

R

P

G

M

V

S

G

M

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

R

A

E

E

N

N

I

M

A

S

Y

F

G

G

L

L

K

K

L

L

R

A

H

G

T

A

K

K

P

K

E

E

E

V

I

I

R

N

E

Q

Q

R

V

V

E

N

I

Q

A

V

L

A

T

E

S

V

M

L

N

Q

L

L

N

A

S

H

L

L

A

L

D

D

R
|
R

A

K

P

P

N

K

E
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

M

L

V

V

M

A

R

E

P

P

K

S

V

V

L

F

L

L

F

L

D

D

E
|
E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

V

V

R

Q

M

M

R

R

L

I

E

E

I

I

R

S

R

R

L

L

Q

H

Q

K

R

R

M

L

G

G

I

R

T

T

S

M

I

I

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

A

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

N

D

A

A

G

G

T

R

I

V

E

A

Q

Q

V

V

A

G

T

K

P

P

E

L

K

E

I

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

V

R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

R

K

A

M

N

N

F

F

L

L

-

P

-

V

-

K

-

V

A

T

A

A

T

T

A

A

R

I

D

D

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F28), S37 (= S57), G38 (= G58), C39 (= C59), G40 (= G60), K41 (= K61), S42 (≠ T62), T43 (= T63), Q81 (= Q101), R128 (= R148), A132 (≠ E152), S134 (= S154), G136 (= G156), Q137 (= Q157), E158 (= E178), H191 (= H211)
binding magnesium ion: S42 (≠ T62), Q81 (= Q101)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
52% identity, 64% coverage: 19:265/386 of query aligns to 3:251/371 of 3puxA

query
sites
3puxA

L

V

E

Q

L

L

R

Q

D

N

V

V

V

T

K

K

V

A

F
x
W

S

G

N

-

R

-

G

-

K

-

D

E

V

V

Y

V

A

V

V

S

H

K

G

D

V

I

S

N

L

L

D

D

I

I

A

H

P

E

G

G

E

E

F

F

V

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

D

T

T

I

T

T

S

S

G

G

Q

D

V

L

V

F

L

I

D

G

G

E

Q

K

N

R

M

M

V

N

S

D

L

T

P

P

N

A

K

E

R

R

P

G

M

V

S

G

M

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

R

A

E

E

N

N

I

M

A

S

Y

F

G

G

L

L

K

K

L

L

R

A

H

G

T

A

K

K

P

K

E

E

E

V

I

I

R

N

E

Q

Q

R

V

V

E

N

I

Q

A

V

L

A

T

E

S

V

M

L

N

Q

L

L

N

A

S

H

L

L

A

L

D

D

R
|
R

A

K

P

P

N

K

E

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

M

L

V

V

M

A

R

E

P

P

K

S

V

V

L

F

L

L

F

L

D

D

E

E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

V

V

R

Q

M

M

R

R

L

I

E

E

I

I

R

S

R

R

L

L

Q

H

Q

K

R

R

M

L

G

G

I

R

T

T

S

M

I

I

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

A

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

N

D

A

A

G

G

T

R

I

V

E

A

Q

Q

V

V

A

G

T

K

P

P

E

L

K

E

I

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

V

R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

R

K

A

M

N

N

F

F

L

L

-

P

-

V

-

K

-

V

A

T

A

A

T

T

A

A

R

I

D

D

binding adenosine-5'-diphosphate: W12 (≠ F28), G38 (= G58), C39 (= C59), G40 (= G60), K41 (= K61), S42 (≠ T62), T43 (= T63), R128 (= R148), S134 (= S154), Q137 (= Q157)
binding beryllium trifluoride ion: S37 (= S57), G38 (= G58), K41 (= K61), Q81 (= Q101), S134 (= S154), G136 (= G156), H191 (= H211)
binding magnesium ion: S42 (≠ T62), Q81 (= Q101)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
52% identity, 64% coverage: 19:265/386 of query aligns to 3:251/371 of 3puwA

query
sites
3puwA

L

V

E

Q

L

L

R

Q

D

N

V

V

V

T

K

K

V

A

F
x
W

S

G

N

-

R

-

G

-

K

-

D

E

V

V

Y

V

A
x
V

V

S

H

K

G

D

V

I

S

N

L

L

D

D

I

I

A

H

P

E

G

G

E

E

F

F

V

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

D

T

T

I

T

T

S

S

G

G

Q

D

V

L

V

F

L

I

D

G

G

E

Q

K

N

R

M

M

V

N

S

D

L

T

P

P

N

A

K

E

R

R

P

G

M

V

S

G

M

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

R

A

E

E

N

N

I

M

A

S

Y

F

G

G

L

L

K

K

L

L

R

A

H

G

T

A

K

K

P

K

E

E

E

V

I

I

R

N

E

Q

Q

R

V

V

E

N

I

Q

A

V

L

A

T

E

S

V

M

L

N

Q

L

L

N

A

S

H

L

L

A

L

D

D

R
|
R

A

K

P

P

N

K

E
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

M

L

V

V

M

A

R

E

P

P

K

S

V

V

L

F

L

L

F

L

D

D

E
|
E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

V

V

R

Q

M

M

R

R

L

I

E

E

I

I

R

S

R

R

L

L

Q

H

Q

K

R

R

M

L

G

G

I

R

T

T

S

M

I

I

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

A

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

N

D

A

A

G

G

T

R

I

V

E

A

Q

Q

V

V

A

G

T

K

P

P

E

L

K

E

I

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

V

R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

R

K

A

M

N

N

F

F

L

L

-

P

-

V

-

K

-

V

A

T

A

A

T

T

A

A

R

I

D

D

binding adenosine-5'-diphosphate: W12 (≠ F28), V17 (≠ A37), G38 (= G58), C39 (= C59), G40 (= G60), K41 (= K61), S42 (≠ T62), T43 (= T63), R128 (= R148), A132 (≠ E152), S134 (= S154), Q137 (= Q157)
binding tetrafluoroaluminate ion: S37 (= S57), G38 (= G58), K41 (= K61), Q81 (= Q101), S134 (= S154), G135 (= G155), G136 (= G156), E158 (= E178), H191 (= H211)
binding magnesium ion: S42 (≠ T62), Q81 (= Q101)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
52% identity, 64% coverage: 19:265/386 of query aligns to 3:251/371 of 3puvA

query
sites
3puvA

L

V

E

Q

L

L

R

Q

D

N

V

V

V

T

K

K

V

A

F
x
W

S

G

N

-

R

-

G

-

K

-

D

E

V

V

Y

V

A
x
V

V

S

H

K

G

D

V

I

S

N

L

L

D

D

I

I

A

H

P

E

G

G

E

E

F

F

V

V

T

V

L

F

L

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

D

T

T

I

T

T

S

S

G

G

Q

D

V

L

V

F

L

I

D

G

G

E

Q

K

N

R

M

M

V

N

S

D

L

T

P

P

N

A

K

E

R

R

P

G

M

V

S

G

M

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

R

A

E

E

N

N

I

M

A

S

Y

F

G

G

L

L

K

K

L

L

R

A

H

G

T

A

K

K

P

K

E

E

E

V

I

I

R

N

E

Q

Q

R

V

V

E

N

I

Q

A

V

L

A

T

E

S

V

M

L

N

Q

L

L

N

A

S

H

L

L

A

L

D

D

R
|
R

A

K

P

P

N

K

E
x
A

L

L

S
|
S

G

G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

M

L

V

V

M

A

R

E

P

P

K

S

V

V

L

F

L

L

F

L

D

D

E

E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

V

V

R

Q

M

M

R

R

L

I

E

E

I

I

R

S

R

R

L

L

Q

H

Q

K

R

R

M

L

G

G

I

R

T

T

S

M

I

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

A

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

N

D

A

A

G

G

T

R

I

V

E

A

Q

Q

V

V

A

G

T

K

P

P

E

L

K

E

I

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

V

R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

R

K

A

M

N

N

F

F

L

L

-

P

-

V

-

K

-

V

A

T

A

A

T

T

A

A

R

I

D

D

binding adenosine-5'-diphosphate: W12 (≠ F28), V17 (≠ A37), G38 (= G58), C39 (= C59), G40 (= G60), K41 (= K61), S42 (≠ T62), T43 (= T63), R128 (= R148), A132 (≠ E152), S134 (= S154), Q137 (= Q157)
binding magnesium ion: S42 (≠ T62), Q81 (= Q101)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
52% identity, 64% coverage: 19:265/386 of query aligns to 1:249/367 of 1q12A

query
sites
1q12A

L

V

E

Q

L

L

R

Q

D

N

V

V

V

T

K

K

V

A

F
x
W

S

G

N

-

R

-

G

-

K

-

D

E

V

V

Y

V

A

V

V

S

H

K

G

D

V

I

S

N

L

L

D

D

I

I

A

H

P

E

G

G

E

E

F

F

V

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

D

T

T

I

T

T

S

S

G

G

Q

D

V

L

V

F

L

I

D

G

G

E

Q

K

N

R

M

M

V

N

S

D

L

T

P

P

N

A

K

E

R

R

P

G

M

V

S

G

M

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

R

A

E

E

N

N

I

M

A

S

Y

F

G

G

L

L

K

K

L

L

R

A

H

G

T

A

K

K

P

K

E

E

E

V

I

I

R

N

E

Q

Q

R

V

V

E

N

I

Q

A

V

L

A

T

E

S

V

M

L

N

Q

L

L

N

A

S

H

L

L

A

L

D

D

R
|
R

A

K

P

P

N

K

E
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

M

L

V

V

M

A

R

E

P

P

K

S

V

V

L

F

L

L

F

L

D

D

E

E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

V

V

R

Q

M

M

R

R

L

I

E

E

I

I

R

S

R

R

L

L

Q

H

Q

K

R

R

M

L

G

G

I

R

T

T

S

M

I

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

A

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

N

D

A

A

G

G

T

R

I

V

E

A

Q

Q

V

V

A

G

T

K

P

P

E

L

K

E

I

L

Y

Y

R

H

R

Y