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Comparing WP_017178483.1 NCBI__GCF_000295095.1:WP_017178483.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6kgyB Hocl-induced flavoprotein disulfide reductase rcla from escherichia coli (see paper)
36% identity, 94% coverage: 24:476/483 of query aligns to 7:434/441 of 6kgyB
- active site: C43 (= C58), C48 (= C63), T51 (= T66), Y168 (≠ V209), E172 (= E213), H426 (= H468), E431 (= E473)
- binding flavin-adenine dinucleotide: I9 (≠ V26), G12 (= G29), E33 (= E50), Q34 (≠ R51), M38 (vs. gap), G41 (= G56), T42 (= T57), G47 (≠ A62), C48 (= C63), A99 (= A140), N126 (= N168), T127 (= T169), G128 (= G170), G291 (= G335), D292 (= D336), F299 (= F343), T300 (= T344), Y301 (≠ H345), S303 (= S347), F333 (= F375)
6kyyA Cu(ii) complex of hocl-induced flavoprotein disulfide reductase rcla from escherichia coli (see paper)
36% identity, 94% coverage: 24:476/483 of query aligns to 3:425/432 of 6kyyA
- active site: C39 (= C58), C44 (= C63), T47 (= T66), Y159 (≠ V209), E163 (= E213), H417 (= H468), E422 (= E473)
- binding copper (ii) ion: C39 (= C58), C44 (= C63), H226 (≠ T279), H229 (≠ G282), T291 (= T344)
- binding flavin-adenine dinucleotide: F7 (≠ G28), G8 (= G29), E29 (= E50), Q30 (≠ R51), M34 (vs. gap), T38 (= T57), C39 (= C58), C44 (= C63), K48 (= K67), A95 (= A140), N117 (= N168), T118 (= T169), G119 (= G170), I160 (= I210), R243 (= R296), D283 (= D336), F290 (= F343), T291 (= T344), S294 (= S347)
P16171 Mercuric reductase; Hg(II) reductase; EC 1.16.1.1 from Bacillus cereus (see paper)
33% identity, 95% coverage: 20:477/483 of query aligns to 169:614/631 of P16171
- Y264 (≠ V116) mutation to F: 30-fold decrease in activity. 300-fold decrease in activity; when associated with F-605.
- Y605 (≠ H468) mutation to F: 10-fold decrease in activity. 300-fold decrease in activity; when associated with F-264.; mutation to H: 2-fold decrease in activity.
8ajjA Crystal structure of the disulfide reductase mera from staphylococcus aureus (see paper)
32% identity, 95% coverage: 22:480/483 of query aligns to 3:437/442 of 8ajjA
- binding flavin-adenine dinucleotide: G10 (= G29), E31 (= E50), Q32 (≠ R51), M36 (vs. gap), G39 (= G56), T40 (= T57), C41 (= C58), C46 (= C63), K50 (= K67), A97 (= A140), N126 (= N168), T127 (= T169), G128 (= G170), I169 (= I210), N255 (≠ V299), G290 (= G335), D291 (= D336), Q297 (= Q342), F298 (= F343), T299 (= T344), Y300 (≠ H345), S302 (= S347)
- binding histidine: D353 (≠ E396), Y354 (≠ V397)
Sites not aligning to the query:
8ajkB Crystal structure of a c43s variant from the disulfide reductase mera from staphylococcus aureus (see paper)
31% identity, 96% coverage: 18:480/483 of query aligns to 2:440/447 of 8ajkB
- binding flavin-adenine dinucleotide: G11 (= G27), G13 (= G29), E34 (= E50), Q35 (≠ R51), M39 (vs. gap), G42 (= G56), T43 (= T57), G48 (≠ A62), C49 (= C63), K53 (= K67), K99 (≠ T139), A100 (= A140), N129 (= N168), T130 (= T169), G131 (= G170), G293 (= G335), D294 (= D336), Q300 (= Q342), F301 (= F343), T302 (= T344), Y303 (≠ H345), S305 (= S347)
8uubA Structure of hypothiocyanous acid reductase (har) from streptococcus pneumoniae (see paper)
36% identity, 94% coverage: 22:476/483 of query aligns to 9:437/442 of 8uubA
- binding flavin-adenine dinucleotide: G14 (= G27), G16 (= G29), E37 (= E50), R38 (= R51), M42 (vs. gap), G45 (= G56), T46 (= T57), G51 (≠ A62), C52 (= C63), K56 (= K67), E101 (≠ T139), A102 (= A140), N129 (= N168), T130 (= T169), G131 (= G170), I172 (= I210), R255 (= R296), G294 (= G335), D295 (= D336), Q301 (= Q342), F302 (= F343), T303 (= T344), Y304 (≠ H345), S306 (= S347)
D9J041 Mercuric reductase; Hg(II) reductase; EC 1.16.1.1 from Lysinibacillus sphaericus (Bacillus sphaericus) (see paper)
33% identity, 95% coverage: 19:477/483 of query aligns to 83:529/546 of D9J041
- C122 (= C58) modified: Disulfide link with 127, Redox-active
- C127 (= C63) modified: Disulfide link with 122, Redox-active
5x1yB Structure of mercuric reductase from lysinibacillus sphaericus (see paper)
33% identity, 95% coverage: 19:477/483 of query aligns to 2:448/454 of 5x1yB
- active site: A13 (≠ K30), V37 (= V54), C41 (= C58), C46 (= C63), S49 (≠ T66), A74 (= A96), G75 (= G97), Y178 (≠ V209), E182 (= E213), A318 (≠ S347), A437 (≠ I466), Y439 (≠ H468), E444 (= E473)
- binding flavin-adenine dinucleotide: I9 (≠ V26), G12 (= G29), I32 (≠ V49), E33 (= E50), R34 (= R51), G39 (= G56), T40 (= T57), C41 (= C58), G45 (≠ A62), C46 (= C63), K50 (= K67), A114 (= A140), T138 (= T169), G139 (= G170), Y178 (≠ V209), R266 (= R296), G305 (= G335), D306 (= D336), F313 (= F343), V314 (≠ T344), A317 (= A346)
2eq6A Crystal structure of lipoamide dehydrogenase from thermus thermophilus hb8
36% identity, 94% coverage: 22:475/483 of query aligns to 5:444/460 of 2eq6A
- active site: V37 (= V54), C41 (= C58), C46 (= C63), T49 (= T66), A176 (≠ V209), E180 (= E213), H435 (≠ I466), H437 (= H468), E442 (= E473)
- binding flavin-adenine dinucleotide: I9 (≠ V26), G10 (= G27), G12 (= G29), P13 (≠ K30), G14 (≠ A31), E33 (= E50), A34 (≠ R51), G39 (= G56), V40 (≠ T57), C41 (= C58), G45 (≠ A62), C46 (= C63), K50 (= K67), F111 (≠ T139), A112 (= A140), A135 (≠ N168), T136 (= T169), G137 (= G170), S155 (= S189), R269 (≠ V299), D306 (= D336), L312 (≠ Q342), L313 (≠ F343), A314 (≠ T344), H315 (= H345), Y344 (≠ F375)
Sites not aligning to the query:
1ebdA Dihydrolipoamide dehydrogenase complexed with the binding domain of the dihydrolipoamide acetylase (see paper)
33% identity, 94% coverage: 20:473/483 of query aligns to 3:445/455 of 1ebdA
- active site: P13 (≠ K30), L37 (≠ V54), C41 (= C58), C46 (= C63), S49 (≠ T66), N74 (≠ Q101), V75 (≠ A102), Y180 (≠ V209), E184 (= E213), S320 (≠ W348), H438 (≠ I466), H440 (= H468), E445 (= E473)
- binding flavin-adenine dinucleotide: G10 (= G27), G12 (= G29), P13 (≠ K30), V32 (= V49), E33 (= E50), K34 (≠ R51), G39 (= G56), V40 (≠ T57), C41 (= C58), G45 (≠ A62), C46 (= C63), K50 (= K67), E112 (≠ T139), A113 (= A140), T141 (= T169), G142 (= G170), Y180 (≠ V209), I181 (= I210), R268 (= R296), D308 (= D336), A314 (≠ Q342), L315 (≠ F343), A316 (≠ T344)
P11959 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
33% identity, 94% coverage: 20:473/483 of query aligns to 9:451/470 of P11959
- 39:47 (vs. 50:58, 44% identical) binding FAD
- K56 (= K67) binding FAD
- D314 (= D336) binding FAD
- A322 (≠ T344) binding FAD
4k8dA Crystal structure of the c558(464)a/c559(465)a double mutant of tn501 mera in complex with NADPH and hg2+
34% identity, 95% coverage: 21:477/483 of query aligns to 4:449/466 of 4k8dA
- active site: G13 (≠ K30), I37 (≠ V54), C41 (= C58), C46 (= C63), S49 (≠ T66), V75 (vs. gap), P76 (vs. gap), V185 (= V209), E189 (= E213), A320 (≠ W348), F438 (≠ I466), Y440 (≠ H468), E445 (= E473)
- binding flavin-adenine dinucleotide: I9 (≠ V26), G10 (= G27), G12 (= G29), A14 (= A31), E33 (= E50), R34 (= R51), G39 (= G56), T40 (= T57), C41 (= C58), G45 (≠ A62), C46 (= C63), K50 (= K67), E115 (≠ T139), A116 (= A140), T145 (= T169), G146 (= G170), R268 (= R296), G307 (= G335), D308 (= D336), F315 (= F343), V316 (≠ T344), Y317 (≠ H345)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: S183 (≠ G207), S184 (≠ G208), V185 (= V209), V186 (≠ I210), E189 (= E213), R206 (≠ G231), N207 (≠ P232), R212 (= R237), T266 (≠ L294), G267 (= G295), Q314 (= Q342), F315 (= F343), V345 (≠ A373)
Sites not aligning to the query:
P00392 Mercuric reductase; Hg(II) reductase; EC 1.16.1.1 from Pseudomonas aeruginosa (see 2 papers)
34% identity, 95% coverage: 21:477/483 of query aligns to 99:544/561 of P00392
- A110 (≠ G32) binding FAD
- G130 (≠ D52) binding FAD
- T135 (= T57) binding FAD
- C136 (= C58) modified: Disulfide link with 141, Redox-active
- C141 (= C63) modified: Disulfide link with 136, Redox-active
- K145 (= K67) binding FAD
- A211 (= A140) binding FAD
- D403 (= D336) binding FAD
- V411 (≠ T344) binding FAD
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 558 binding Hg(2+)
- 559 binding Hg(2+)
3urhB Crystal structure of a dihydrolipoamide dehydrogenase from sinorhizobium meliloti 1021
34% identity, 94% coverage: 22:476/483 of query aligns to 2:453/465 of 3urhB
- active site: Y35 (≠ V54), C39 (= C58), C44 (= C63), S47 (≠ T66), V183 (= V209), E187 (= E213), H443 (≠ I466), H445 (= H468), E450 (= E473)
- binding flavin-adenine dinucleotide: I6 (≠ V26), G7 (= G27), G9 (= G29), P10 (≠ K30), G11 (≠ A31), E30 (= E50), K31 (vs. gap), G37 (= G56), T38 (= T57), C39 (= C58), G43 (≠ A62), C44 (= C63), K48 (= K67), T111 (= T139), G112 (≠ A140), A140 (≠ N168), T141 (= T169), G142 (= G170), I184 (= I210), R273 (= R296), G312 (= G335), D313 (= D336), M319 (≠ Q342), L320 (≠ F343), A321 (≠ T344), H322 (= H345)
8qcjA Crystal structure of mycothiol disulfide reductase mtr from rhodococcus erythropolis (see paper)
34% identity, 94% coverage: 22:473/483 of query aligns to 5:447/458 of 8qcjA
- binding flavin-adenine dinucleotide: G10 (= G27), G12 (= G29), L30 (≠ V49), E31 (= E50), E32 (≠ R51), G37 (= G56), T38 (= T57), G43 (≠ A62), A113 (= A140), A138 (≠ T169), G139 (= G170), N158 (≠ S189), F178 (≠ V209), I179 (= I210), D302 (= D336), Q308 (= Q342), L309 (≠ F343), K310 (≠ T344), H311 (= H345)
2yquB Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
34% identity, 94% coverage: 22:476/483 of query aligns to 3:442/455 of 2yquB
- active site: P11 (≠ K30), L36 (≠ V54), C40 (= C58), C45 (= C63), S48 (≠ T66), G72 (= G86), V73 (= V87), V177 (= V209), E181 (= E213), S314 (= S347), H432 (≠ I466), H434 (= H468), E439 (= E473)
- binding carbonate ion: A310 (≠ T344), S314 (= S347), S423 (≠ T457), D426 (≠ Q460)
- binding flavin-adenine dinucleotide: G8 (= G27), G10 (= G29), P11 (≠ K30), G12 (≠ A31), E31 (= E50), K32 (≠ R51), G38 (= G56), T39 (= T57), C40 (= C58), R42 (≠ N60), G44 (≠ A62), C45 (= C63), K49 (= K67), T110 (= T139), A111 (= A140), T137 (= T169), G138 (= G170), I178 (= I210), Y265 (≠ V299), G301 (= G335), D302 (= D336), M308 (≠ Q342), L309 (≠ F343), A310 (≠ T344), H311 (= H345)
2yquA Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
34% identity, 94% coverage: 22:476/483 of query aligns to 3:442/455 of 2yquA
- active site: P11 (≠ K30), L36 (≠ V54), C40 (= C58), C45 (= C63), S48 (≠ T66), G72 (= G86), V73 (= V87), V177 (= V209), E181 (= E213), S314 (= S347), H432 (≠ I466), H434 (= H468), E439 (= E473)
- binding flavin-adenine dinucleotide: G8 (= G27), G10 (= G29), P11 (≠ K30), G12 (≠ A31), E31 (= E50), K32 (≠ R51), G38 (= G56), T39 (= T57), C40 (= C58), R42 (≠ N60), G44 (≠ A62), C45 (= C63), K49 (= K67), T110 (= T139), A111 (= A140), T137 (= T169), G138 (= G170), S157 (= S189), I178 (= I210), Y265 (≠ V299), G301 (= G335), D302 (= D336), M308 (≠ Q342), L309 (≠ F343), A310 (≠ T344)
2eq7A Crystal structure of lipoamide dehydrogenase from thermus thermophilus hb8 with psbdo
34% identity, 94% coverage: 22:476/483 of query aligns to 3:442/452 of 2eq7A
- active site: P11 (≠ K30), L36 (≠ V54), C40 (= C58), C45 (= C63), S48 (≠ T66), G72 (= G86), V73 (= V87), V177 (= V209), E181 (= E213), S314 (= S347), H432 (≠ I466), H434 (= H468), E439 (= E473)
- binding flavin-adenine dinucleotide: G10 (= G29), P11 (≠ K30), G12 (≠ A31), E31 (= E50), K32 (≠ R51), G38 (= G56), T39 (= T57), C40 (= C58), R42 (≠ N60), G44 (≠ A62), C45 (= C63), K49 (= K67), T110 (= T139), A111 (= A140), T137 (= T169), G138 (= G170), S157 (= S189), I178 (= I210), R262 (= R296), Y265 (≠ V299), D302 (= D336), M308 (≠ Q342), L309 (≠ F343), A310 (≠ T344), H311 (= H345), Y341 (≠ F375)
- binding nicotinamide-adenine-dinucleotide: W146 (≠ I178), G174 (= G206), G176 (= G208), V177 (= V209), I178 (= I210), E197 (≠ H229), Y198 (≠ A230), V231 (≠ A263), V260 (≠ L294), G261 (= G295), R262 (= R296), M308 (≠ Q342), L309 (≠ F343), V339 (≠ A373)
6uziC Crystal structure of dihydrolipoyl dehydrogenase from elizabethkingia anophelis nuhp1
31% identity, 96% coverage: 18:483/483 of query aligns to 4:464/470 of 6uziC
- active site: C45 (= C58), C50 (= C63), S53 (≠ T66), V187 (= V209), E191 (= E213), H448 (= H468), E453 (= E473)
- binding flavin-adenine dinucleotide: I12 (≠ V26), G13 (= G27), G15 (= G29), P16 (≠ K30), G17 (≠ A31), E36 (= E50), K37 (≠ R51), G43 (= G56), T44 (= T57), C45 (= C58), G49 (≠ A62), C50 (= C63), S53 (≠ T66), K54 (= K67), V117 (≠ T139), G118 (≠ A140), T147 (= T169), G148 (= G170), I188 (= I210), R276 (= R296), D316 (= D336), M322 (≠ Q342), L323 (≠ F343), A324 (≠ T344)
- binding zinc ion: H448 (= H468), E453 (= E473)
4k7zA Crystal structure of the c136(42)a/c141(47)a double mutant of tn501 mera in complex with NADP and hg2+
34% identity, 95% coverage: 21:477/483 of query aligns to 5:450/467 of 4k7zA
- active site: G14 (≠ K30), I38 (≠ V54), A42 (≠ C58), A47 (≠ C63), S50 (≠ T66), V76 (vs. gap), P77 (vs. gap), V186 (= V209), E190 (= E213), A321 (≠ W348), F439 (≠ I466), Y441 (≠ H468), E446 (= E473)
- binding flavin-adenine dinucleotide: I10 (≠ V26), G11 (= G27), G13 (= G29), A15 (= A31), E34 (= E50), R35 (= R51), G40 (= G56), T41 (= T57), A42 (≠ C58), G46 (≠ A62), A47 (≠ C63), K51 (= K67), E116 (≠ T139), A117 (= A140), T146 (= T169), G147 (= G170), R269 (= R296), G308 (= G335), D309 (= D336), Q315 (= Q342), F316 (= F343), V317 (≠ T344), Y318 (≠ H345)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S184 (≠ G207), S185 (≠ G208), V186 (= V209), V187 (≠ I210), E190 (= E213), R207 (≠ G231), N208 (≠ P232), R213 (= R237), T267 (≠ L294), G268 (= G295), R269 (= R296), Q315 (= Q342), F316 (= F343), V346 (≠ A373)
Sites not aligning to the query:
Query Sequence
>WP_017178483.1 NCBI__GCF_000295095.1:WP_017178483.1
MSQSTVNSDATNQTDVPVEEVDLLVVGGGKAGKSLAMLRAKAGDKVVMVERDKVGGTCIN
VACIPTKTLISAARVLREVQGSQAYGVTLSEQDGGAGALAQARIELASFRARKEAVVGGM
VAAHEKMFPASGMDFVKGTARFVGERTVEIALNDGGLRRVRGAKVLINTGTTPSVPSIEG
LSDVRYWTSEDLLTLPELPSSLIVLGGGVIGVEMASLMGLLGVPVTIVHAGPHILDREDE
DVAAEVTAGLEALGVTVLAGAPASKVAAAADGNGVVVTTADGHEVSGSHLLVALGRTPVT
AGLGLEAAGVELTERGFVRVDDHLRTTAENVFAAGDVAGTPQFTHASWNDFRVLRDLFAG
KEASTTGRLIPWAVFTTPELGHVGLSEAEAREAGYEVRVAKTPTAAVPRAKTLGHTEGFF
KVIIDARTDLILGAAIIGAEASEVVTSIQMAMLGDLTWQQVRDAVITHPTMSEGLNIVLD
SLG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory