SitesBLAST

M1 (≠ V10) modified: Initiator methionine, Removed
Y102 (= Y111) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (= W115) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K172) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (≠ Y224) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E230) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E238) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (≠ A283) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (≠ N286) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.
E438 (≠ G446) mutation to A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
D443 (≠ G451) mutation to A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
D446 (≠ S453) mutation to A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
38% identity, 97% coverage: 9:484/493 of query aligns to 69:550/587 of Q9URZ4

query
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Q9URZ4

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Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
38% identity, 99% coverage: 2:491/493 of query aligns to 64:569/590 of P04817

query
sites
P04817

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P113 (≠ T51) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (= P86) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (= V87) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (= S90) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (= Y111) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (= G237) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (= P242) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
T-S 354:355 (≠ AED 283:285) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (≠ N286) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
W451 (≠ F381) mutation to C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; mutation to L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; mutation to S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
F461 (= F391) mutation to S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
34% identity, 96% coverage: 5:479/493 of query aligns to 60:552/602 of P19145

query
sites
P19145

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F

W

W

L

G

P

T

D

D

V

-

P

P

S

K

L

Y

V

R

-

D

-

G

W

F

S

V

A

A

L

L

F

F

L

W

V

L

I

A

L

I

F

V

T

I

I

I

N

N

A

M

L

F

S

G

A

V

R

K

A

G

Y

Y

G

G

E

E

S

A

E

E

F

F

L

V

F

F

A

S

S

F

I

I

K

K

V

V

V

I

A

T

V

V

I

V

V

G

F

F

L

I

I

I

L

L

G

G

V

I

A

I

M

L

I

N

A

C

G

G

I

G

L

G

G

P

G

T

P

G

S

G

P

Y

G

I

T

G

E

G

N

K

W

Y

T

W

T

H

G

D

E

P

A

G

P

A

F

F

V

A

G

G

G

D

G

T

-

P

-

G

-

A

-

K

-

F

E

K

G

G

I

V

L

C

L

S

V

V

L

F

L

V

V

T

A

A

G

A

Y

F

S

S

F

F

Q
x
A

G

G

T

S

E

E

L

L

I

V

G

G

T

L

A

A

G

S

E

E

A

S

E

V

N

E

P

P

E

R

R

K

T

S

I

V

P

P

R

K

A

A

I

A

R

K

T

Q

I

V

F

F

W

W

R

R

I

I

L

T

L

L

F

F

Y

Y

I

I

G

L

A

S

I

L

A

L

V

M

I

I

G

G

F

L

L

L

I

V

P

P

Y

Y

T

N

D

D

P

K

N

S

L

L

L

I

N

G

S

A

A

S

E

S

D

V

N

D

V

A

S

A

V

A

S

S

P

P

F

F

T

V

L

I

V

A

F

I

E

K

R

T

A

H

G

G

I

I

L

K

G

G

A

L

A

P

S

S

V

V

I

V

N

N

A

V

I

V

I

I

L

L

T

I

S

A

V

V

L

L

S

S

A

V

G

G

T

N

S

S

G

A

L

I

Y

Y

S

A

S

C

T

S

R

R

M

T

L

M

F

V

A

A

L

L

A

A

E

E

R

Q

G

R

H

F

A

L

P

P

R

E

F

I

L

F

T

S

R

Y

L

V

S

D

S

R

H

K

Q

G

V

R

P

P

M

L

N

V

A

G

L

I

V

A

A

V

T

T

T

S

L

A

V

F

G

G

L

L

A

I

G

A

F

F

I

V

T

A

S

A

L

S

V

K

G

K

D

E

G

G

A

E

A

V

Y

F

E

N

F

W

L

L

T

A

L

L

S

S

A

G

L

L

A

S

G

S

F

L

I

F

T

T

W

W

A

G

G

G

I

I

S

C

W

I

C

C

H

H

W

I

R

R

F

F

R

R

M

K

A

A

L

L

K

A

A

A

Q

Q

G

G

Q

R

P

G

L

L

T

D

D

E

L

L

P

S

Y

F

R

K

A

S

R

P

F

T

F

G

P

V

A

W

G

G

A

S

I

Y

V

W

A

G

L

L

I

F

A

M

C

V

I

I

A

I

I

M

I

F

I

I

G

A

Q

Q

A

F

Y

Y

-

V

-

A

-

V

G

F

P

P

V

V

T

G

S

D

G

S

K

P

S

S

L

A

G

E

E

G

I

F

L

F

M

E

P

A

Y

Y

I

L

G

S

I

F

P

P

V

L

F

V

L

M

A

V

L

M

W

Y

W

I

G

G

H

H

K

K

L

I

V

Y

T

K

R

R

K76 (≠ E21) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
A297 (≠ Q227) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

P24207 Phenylalanine-specific permease; Phenylalanine:H(+) symporter PheP from Escherichia coli (strain K12) (see 3 papers)
39% identity, 88% coverage: 5:440/493 of query aligns to 2:421/458 of P24207

query
sites
P24207

T

K

Q

N

A

A

G

S

P

T

V

V

A

S

A

E

D

D

G

T

E

A

Q

S

S

N

E

Q

P

E

Q

P

E

T

L

L

R

H

R

R

S

G

L

L

L

H

S

N

R
|
R

H

H

L

I

T

Q

M

L

I

I

S

A

I

L

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

F

V

L

A

G

S

I

G

G

A

P

T

A

I

I

S

Q

Q

M

A

A

G

G

P
|
P

G

A

G

-

A

V

L

L

V

L

A

G

Y

Y

A

G

A

V

V

A

G

G

I

I

M

I

V

A

W

F

L

L

I

I

M

M

Q

R

S

Q

L

L

G

G

E

E

M

M

A

V

Y

E

L

E

P

P

V

V

A

S

G

G

S

S

F
|
F

G

A

E

H

Y
x
F

G

A

T
x
Y

R

K

F
x
Y

V
x
W

S

G

P

P

S
x
F

F

A

G

G

F
|
F

A

L

I

S

G

G

W
|
W

N

N

Y
|
Y

W
|
W

F

V

N

M

W
x
F

A

V

I

L

T

V

V

G

A

M

A

A

E
|
E

L

L

V

T

A

A

A

G

L

I

V

Y

M

M

K

Q

Y

Y

W

W

L

F

P

P

D

D

V

V

P

P

S

T

L

W

V

I

W

W

S

A

A

A

L

A

F

F

L

F

V

I

I

I

L

I

F

N

T

A

I

V

N

N

A

L

L

V

S

N

A

V

R

R

A

L

Y

Y

G

G

E

E

S

T

E

E

F

F

L

W

F

F

A

A

S

L

I

I

K
|
K

V

V

V

L

A

A

V

I

I

I

V

G

F

M

L

I

I

G

L

F

G

G

V

L

A

W

M

L

I

L

A

F

G

S

I

G

L

H

G

G

P

E

S

K

P

A

G

S

T

I

E

D

N

N

-

L

W

W

T

R

T

Y

G

G

E

-

A

G

P

F

F

F

V

A

G

T

G

G

G

W

E

N

G

G

I

L

L

I

L

L

V

S

L

L

L

A

V

V

A

I

G

M

Y

F

S

S

F

F

Q

G

G

G

T

L

E
|
E

L

L

I

I

G

G

T

I

A

T

A

A

G

A

E

E

A

A

E

R

N

D

P

P

E

E

R

K

T

S

I

I

P

P

R

K

A

A

I

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R

N

T

Q

I

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F

V

W

Y

R
|
R

I

I

L

L

F

F

Y

Y

I

I

G

G

A

S

I

L

A

V

V

V

I

L

G

L

F

A

L

L

I

Y

P

P

Y

W

T

V

D

-

P

-

N

-

L

-

N

-

S

-

A

-

E

E

D

V

N

K

V

S

S

N

V

S

S

S

P

P

F

F

T

V

L

M

V

I

F

F

E

H

R

N

A

L

G

D

I

S

L

N

G

V

A

V

A

A

S

S

V

A

I

L

N

N

A

F

I

V

I

I

L

L

T

V

S

A

V

S

L

L

S

S

A

V

G

Y

T

N

S

S

G

G

L

V

Y

Y

S

S

S

N

T

S

R

R

M

M

L

L

F

F

A

G

L

L

A

S

E

V

R

Q

G

G

H

N

A

A

P

P

R

K

F

F

L

L

T

T

R

R

L

V

S

S

S

R

H

R

Q

G

V

V

P
|
P

M

I

N

N

A

S

L

L

V

M

A

-

T

-

L

-

V

-

G

-

L

L

A

S

G

G

F

A

I

I

T

T

S

S

L

L

V

V

G

V

-

L

-

I

-

N

-

Y

-

L

D

P

G

Q

A

K

A

A

Y

F

E

G

F

L

L

L

L

M

T

A

L

L

S

V

A

V

L

A

A

T

G

L

F

L

I

L

T

N

W

W

A

I

G

M

I

I

S

C

W

L

C

A

H

H

W

L

R

R

F

F

R

R

M

A

A

A

L

M

K

R

A

R

Q

Q

G

G

Q

R

P

-

L

-

T

-

D

E

L

T

P

Q

Y

F

R

K

A

A

R

L

F

L

F

Y

P

P

A

F

G

G

A

N

I

Y

V

L

A

-

L

-

I

-

A

-

C

C

I

I

A

A

I

F

I

L

R26 (= R29) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P57) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (= F91) mutation to L: No effect on phenylalanine transport activity.
F90 (≠ Y94) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ T96) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (≠ F98) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ V99) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ S102) mutation to L: No effect on phenylalanine transport activity.
F101 (= F105) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W109) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (= Y111) mutation to L: No effect on phenylalanine transport activity.
W108 (= W112) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ W115) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (= E122) mutation E->G,L,V,N: Loss of activity.
K168 (= K172) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E230) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R256) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P353) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.

Sites not aligning to the query:

442 P→A: 46% of wild-type phenylalanine transport activity.; P→G: 52% of wild-type phenylalanine transport activity.; P→L: 43% of wild-type phenylalanine transport activity.

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
39% identity, 86% coverage: 22:445/493 of query aligns to 8:421/469 of P46349

query
sites
P46349

L

L

R

K

S

E

L

L

L

K

S

T

R

R

H

H

L

M

T

T

M

M

I

I

S

S

I

I

G

A

G

G

A

V

I

I

G

G

T

A

G

G

L

L

F

F

V

V

A
x
G

S

S

G

G

A

S

T

V

I

I

S

H

Q

S

A

T

G
|
G

P

P

G

G

G

-

A

A

L

V

V

V

A

S

Y

Y

A

A

A

L

V

A

G

G

I

L

M

L

V

V

W

I

L

F

I

I

M

M

Q

R

S

M

L

L

G

G

E

E

M

M

A

S

A

A

Y

V

L

N

P

P

V

T

A

S

G

G

S

S

F

F

G

S

E

Q

Y

Y

G

A

T

H

R

D

F

A

V

I

S

G

P

P

S

W

F

A

G

G

F

F

A

T

I

I

G

G

W

W

N

L

Y

Y

W

W

F

F

N

F

W

W

A

V

I

I

T

V

V

I

A

A

A

I

E

E

L

A

V

I

A

A

A

G

A

A

L

G

V

I

M

I

K

Q

Y

Y

W

W

L

F

P

H

D

D

V

I

P

P

S

L

L

W

V

L

W

T

S

S

A

L

L

I

F

L

L

T

V

I

I

V

L

L

F

T

T

L

I

T

N

N

A

V

L

Y

S

S

A

V

R

K

A

S

Y

F

G

G

E

E

S

F

E

E

F

Y

L

W

F

F

A

S

S

L

I

I

K

K

V

V

A

T

V

I

I

I

V

A

F

F

L

L

I

I

L

V

G

G

V

F

A

A

M

F

I

I

A

F

G

G

I

F

L

A

G

P

G

G

P

S

S

E

P

P

-

V

G

G

T

F

E

S

N

N

W

L

T

T

T

G

G

K

E

G

A

G

P

F

F

F

V

P

G

E

G

G

G

I

E

S

G

S

I

V

L

L

V

G

L

I

L

V

V

V

A

V

G

I

Y

F

S

S

F

F

Q

M

G

G

T

T

E

E

L

I

I

V

G

A

T

I

A

A

G

G

E

E

A

T

E

S

N

N

P

P

E

I

R

E

T

S

I

V

P

T

R

K

A

A

I

T

R

R

T

S

I

V

F

V

W

W

R

R

I

I

L

I

L

V

F

F

Y

Y

I

V

G

G

A

S

I

I

A

A

V

I

I

V

G

V

F

A

L

L

I

L

P

P

Y

W

T

N

D

S

P

A

N

N

L

I

L

L

N

E

S

-

A

-

E

-

D

-

N

-

V

-

S

-

V

-

S

S

P

P

F

F

T

V

L

A

V

V

F

L

E

E

R

H

A

I

G

G

I

V

L

P

G

A

A

A

S

Q

V

I

I

M

N

N

A

F

I

I

I

V

L

L

T

T

S

A

V

V

L

L

S

S

A

C

G

L

T

N

S

S

G
|
G

L

L

Y

Y

S

T

T

S

R

R

M

M

L

L

F

Y

A

S

L

L

A

A

E

E

R

R

G

N

H

E

A

A

P

P

R

R

F

R

L

F

T

M

R

K

L

L

S

S

S

K

H

K

Q

G

V

V

P

P

M

V

N

Q

A

A

L

I

V

V

A

A

T
x
G

T

T

L

F

V
x
F

G

S

L

Y

A

I

G

A

F

V

I

V

T

M

S

N

L

Y

V

F

G

S

D

P

G

D

A

T

A

V

Y

F

E

L

F

F

L

L

L

V

T

N

L

S

S

S

A

G

L

A

A

I

G

A

F

L

I

L

T

V

W

Y

A

L

G

V

I

I

S

A

W

V

C

S

H

Q

W

L

R

K

F

M

R

R

M

K

A

K

L

L

K

E

A

-

Q

K

G

T

Q

N

P

P

L

-

T

E

D

A

L

L

P

K

Y

I

R

K

A

M

R

W

F

L

F

F

P

P

A

F

G

L

A

T

I

Y

V

L

A

T

L

I

I

I

A

A

C

I

I

C

A
x
G

I

I

I

L

I

V

G

S

Q

M

A

A

Y

F

G33 (≠ A47) mutation to D: Lack of activity.
G42 (= G56) mutation to S: Lack of activity.
G301 (= G323) mutation to V: Lack of activity.
G338 (≠ T360) mutation to E: Lack of activity.
F341 (≠ V363) mutation to S: Lack of activity.
G414 (≠ A438) mutation to R: Lack of activity.

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
37% identity, 86% coverage: 16:441/493 of query aligns to 5:418/457 of P15993

query
sites
P15993

Q

Q

S

Q

E

H

P

G

Q

E

E

Q

L

L

R

K

R

R

S

G

L

L

L

K

S

N

R

R

H

H

L

I

T

Q

M

L

I

I

S

A

I

L

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

F

V

L

A

G

S

S

G

A

A

S

T

V

I

I

S

Q

Q

S

A

A

G

G

P

P

G

G

G

-

A

I

L

I

V

L

A

G

Y

Y

A

A

A

I

V

A

G

G

I

F

M

I

V

A

W

F

L

L

I

I

M

M

Q

R

S

Q

L

L

G

G

E

E

M

M

A

V

Y

E

L

E

P

P

V

V

A

A

G

G

S

S

F

F

G

S

E

H

Y

F

G

A

T

Y

R

K

F

Y

V

W

S

G

P

S

S

F

F

A

G

G

F

F

A

A

I

S

G

G

W

W

N

N

Y

Y

W

W

F

V

N

L

W
x
Y

A

V

I

L

T

V

V

A

A

M

A

A

E

E

L

L

V

T

A

A

A

V

A

G

L

K

V

Y

M

I

K

Q

Y

F

W

W

L

Y

P

P

D

E

V

I

P

P

S

T

L

W

V

V

W

S

S

A

A

A

L

V

F

F

L

F

V

V

I

V

L

I

F

N

T

A

I

I

N

N

A

L

L

T

S

N

A

V

R

K

A

V

Y

F

G

G

E

E

S

M

E

E

F

F

L

W

F

F

A

A

S

I

I

I

K

K

V

V

V

I

A

A

V

V

I

V

V

A

F

M

L

I

I

I

L

F

G

G

V

G

A

W

M

L

I

L

A

F

G

S

I

G

L

N

G

G

P

P

S

Q

P

A

G

T

T

V

E

S

N

N

-

L

W

W

T

D

T

Q

G

G

E

-

A

G

P

F

F

L

V

P

G

H

G

G

G

F

E

T

G

G

I

L

L

V

L

M

V

M

L

M

L

A

V

I

A

I

G

M

Y

F

S

S

F

F

Q

G

G

G

T

L

E

E

L

L

I

V

G

G

T

I

A

T

A

A

G

A

E

E

A

A

E

D

N

N

P

P

E

E

R

Q

T

S

I

I

P

P

R

K

A

A

I

T

R

N

T

Q

I

V

F

I

W

Y

R

R

I

I

L

L

L

I

F

F

Y

Y

I

I

G

G

A

S

I

L

A

A

V

V

I

L

G

L

F

S

L

L

I

M

P

P

Y

W

T

T

D

-

P

-

N

-

L

-

N

-

S

-

A

-

E

R

D

V

N

T

V

A

S

D

V

T

S

S

P

P

F

F

T

V

L

L

V

I

F

F

E

H

R

E

A

L

G

G

I

D

L

T

G

F

A

V

A

A

S

N

V

A

I

L

N

N

A

I

I

V

L

L

T

T

S

A

V

A

L

L

S

S

A

V

G

Y

T

N

S

S

G

C

L

V

Y

Y

S

C

S

N

T

S

R

R

M

M

L

L

F

F

A

G

L

L

A

A

E

Q

R

Q

G

G

H

N

A

A

P

P

R

K

F

A

L

L

T

A

R

S

L

V

S

D

S

K

H

R

Q

G

V

V

P

P

M

V

N

N

A

T

L

I

V

L

A

V

T

S

T

A

L

L

V

V

G

T

L

A

A

L

G

C

F

V

I

L

T

I

S

N

L

Y

V

L

G

A

D

P

G

E

A

S

A

A

Y

F

E

G

F

L

L

L

L

M

T

A

L

L

S

V

A

V

L

S

A

A

G

L

F

V

I

I

T

N

W

W

A

A

G

M

I

I

S

S

W

L

C

A

H

H

W

M

R

K

F

F

R

R

M

R

A

A

L

K

K

Q

A

E

Q

Q

G

G

Q

-

P

V

L

V

T

T

D

R

L

F

P

P

Y

-

R

-

A

A

R

L

F

L

F

Y

P

P

A

L

G

G

A

N

I

W

V

I

A

C

L

L

I

L

A

F

C

M

I

A

A

A

I

V

I

L

I

V

Y103 (≠ W115) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
30% identity, 96% coverage: 4:476/493 of query aligns to 120:610/663 of P48813

query
sites
P48813

S

N

T

R

Q

K

A

S

G

S

P

Y

V

I

A

T

A

V

D

D

G

G

E

I

Q
x
K

S

Q

E

S

P

P

Q

Q

E

E

-

Q

-

E

-

Q

-

K

-

Q

-

E

-

N

L

L

R

K

S

S

L

I

L

K

S

P

R

R

H

H

L

T

T

V

M

M

I

M

S

S

I

L

G

G

G

T

A

G

I

I

G

G

T

T

G

G

L

L

F

L

V

V

A

G

S

N

G

S

A

K

T

V

I

L

S

N

Q

N

A

A

G

G

P

P

G

G

A

L

L

I

V

I

A

G

Y

Y

A

A

A

I

V

M

G

G

I

S

M

C

V

V

W

Y

L

C

I

I

M

I

Q

Q

S

A

L

C

G

G

E

E

M

L

A

A

A

V

-

I

Y

Y

L

S

P

D

V

L

A

I

G

G

S

G

F

F

G

N

E

T

Y

Y

G

P

T

L

R

F

F

L

V

V

S

D

P

P

S

A

F

L

G

G

F

F

A

S

I

V

G

A

W

W

N

L

Y

F

W

C

F

L

N

Q

W

W

A

L

I

C

T

V

V

C

A

P

A

L

E

E

L

L

V

V

A

T

A

A

A

S

L

M

V

T

M

I

K

K

Y

Y

W

W

L

T

P

T

D

S

V

V

P

N

S

P

L

D

V

V

W

F

S

V

A

V

L

I

F

F

L

Y

V

V

I

L

L

I

F

V

T

V

I

I

N

N

A

V

L

F

S

G

A

A

R

K

A

G

Y

Y

G

A

E

E

S

A

E

D

F

F

L

F

F

F

A

N

S

C

I

C

K

K

V

I

V

L

A

M

V

I

I

V

V

G

F

F

L

F

I

I

L

L

G

A

V

I

A

I

M

I

I

D

A

C

G

G

I

G

L

A

G

G

G

T

P

D

S

G

P

Y

-

I

G

G

T

S

E

K

N

Y

W

W

T

R

T

D

-

P

-

G

-

A

-

F

-

R

G

G

E

D

A

T

P

P

F

-

V

I

G

Q

G

R

G

F

E

K

G

G

I

V

L

V

L

A

V

T

L

F

L

V

V

T

A

A

G

A

Y

F

S

A

F

F

Q

G

G

M

T

S

E

E

L

Q

I

L

G

A

T

M

A

T

A

A

G

S

E

E

A

Q

E

S

N

N

P

P

E

R

R

K

T

A

I

I

P

P

R

S

A

A

I

A

R

K

T

K

I

M

F

I

W

Y

R

R

I

I

L

L

L

F

F

V

Y

F

I

L

G

A

A

S

I

L

A

T

V

L

I

V

G

G

F

F

L

L

I

V

P

P

Y

Y

T

T

D

S

P

D

N

Q

L

L

N

G

S

A

A

A

E

G

D

S

N

A

V

T

S

K

V

A

S

S

P

P

F

Y

T

V

L

I

V

A

F

V

E

S

R

S

A

H

G

G

I

V

L

R

G

V

A

V

A

P

S

H

V

F

I

I

N

N

A

A

I

V

I

I

L

L

T

L

S

S

V

V

L

L

S

S

A

V

G

A

T

N

S

G

G

A

L

F

Y

Y

S

T

S

S

T

S

R

R

M

I

L

L

F

M

A

S

L

L

A

A

E

K

R

Q

G

G

H

N

A

A

P

P

R

K

F

C

L

F

T

D

R

Y

L

I

S

D

S

R

H

E

Q

G

V

R

P

P

M

A

N

A

A

A

L

M

V

L

A

V

T

S

T

A

L

L

V

F

G

G

L

V

A

I

G

A

F

F

I

C

T

A

S

S

L

S

V

K

G

K

D

E

G

E

A

D

A

V

Y

F

E

T

F

W

L

L

T

A

L

I

S

S

A

G

L

L

A

S

G

Q

F

L

I

F

T

T

W

W

A

I

G

T

I

I

S

C

W

L

C

S

H

H

W

I

R

R

F

F

R

R

M

R

A

A

L

M

K

K

A

V

Q

Q

G

G

Q

R

P

S

L

L

T

G

D

E

L

V

P

G

Y

Y

R

K

A

S

R

Q

F

V

F

G

P

V

A

W

G

G

A

S

I

A

V

Y

A

A

L

V

I

L

A

M

C

M

I

V

A

L

I

A

I

L

I

I

G

A

Q

Q

-

F

-

W

-

V

A

A

Y

I

G

A

P

P

V

I

T

G

S

G

G

G

K

G

S

K

L

L

G

S

-

A

-

Q

E

S

I

F

L

F

M

E

P

N

Y

Y

I

L

G

A

I

M

P

P

V

I

F

W

L

I

A

A

L

L

W

Y

W

I

G

F

H

Y

K

K

L

V

K132 (≠ Q16) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q88CZ8 L-histidine transporter HutT from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
30% identity, 97% coverage: 17:492/493 of query aligns to 2:467/467 of Q88CZ8

query
sites
Q88CZ8

S

Q

E

Q

P

A

Q

Q

E

G

L

L

R

K

R

R

S

G

L

L

L

S

S

A

R

R

H

H

L

I

T

R

M

F

I

M

S

A

I

L

G

G

G

S

A

A

I

I

G

G

T
|
T

G

G

L

L

F

F

V

Y

A

G

S

S

G

A

A

S

T

A

I

I

S

Q

Q

M

A

A

G

G

P

P

G

A

G

-

A

V

L

L

V

L

A

A

Y

Y

A

L

A

I

V

G

G

G

I

A

M

A

V

V

W

F

L

M

I

V

M

M

Q

R

S

A

L

L

G

G

E

E

M

M

A

A

A

V

Y

H

L

N

P

P

V

V

A

A

G

G

S

S

F

F

G

G

E

H

Y

Y

G

A

T

T

R

T

F

Y

V

L

S

G

P

P

S

M

F

A

G

G

F

F

A

I

I

L

G

G

W

W

N

T

Y

Y

W

A

F

F

N
x
E

W

M

A

V

I

I

T

V

V

A

A

I

A

A

E

D

L

V

V

T

A

A

A

F

A

G

L

I

V

Y

M

M

K

G

Y

F

W

W

L

F

P

P

D

E

V

V

P

A

S

R

L

W

V

I

W

W

S

V

A

L

L

G

F

I

L

V

V

F

I

L

L

I

F

G

T

G

I

L

N

N

A

L

L

C

S

N

A

V

R

K

A

V

Y

F

G

G

E

E

S

M

E

E

F

F

L

W

F

L

A

S

S

L

I

L

K
|
K

V

V

V

G

A

A

V

I

I

V

V

A

F

M

L

I

I

L

L

A

G

G

V

L

A

G

M

I

I

M

A

A

-

F

-

G

-

F

-

S

G

Q

I

V

L

G

G

T

G

G

P

H

S

A

P

V

G

G

T

M

E

S

N

N

W

L

T

F

T

D

G

H

E

G

A

G

P

F

F

M

V

P

G

N

G

G

G

V

E

G

G

G

I

L

L

I

L

A

V

S

L

F

L

A

V

V

A

V

G

M

Y
x
F

S

A

F

F

Q

G

G

G

T

I

E
|
E

L

I

I

I

G

G

T

V

A

T

A

A

G

G

E

E

A

A

E

K

N

D

P

P

E

Q

R

R

T

V

I

I

P

P

R

K

A

A

I

I

R

N

T

A

I

V

F

P

W

L

R

R

I

I

L

L

F

F

Y

Y

I

V

G

L

A

T

I

L

A

F

V

V

I

L

G

M

F

C

L

L

I

Y

P

P

Y

W

T

-

D

-

P

P

N

Q

L

I

L

G

N

S

S

Q

A

G

E

-

D

-

N

-

V

-

S

-

V

-

S

S

P

P

F

F

T

V

L

Q

V

I

F

F

E

S

R

N

A

L

G

G

I

I

L

G

G

S

A

A

S

A

V

V

I

L

N

N

A

V

I

V

L

I

T

S

S

A

V

A

L

I

S

S

A

A

G

I

T

N

S

S

G

D

L

I

Y

F

S

G

S

A

T

G

R

R

M

M

L

M

F

Y

A

G

L

L

A

A

E

Q

R

Q

G

G

H

H

A

A

P

P

R

R

F

G

L

F

T

S

R

K

L

L

S

S

S

K

H

H

Q

G

V

V

P

P

M

W

N

M

A

T

L

V

V

V

A

V

T

M

T

G

L

A

V

A

G

L

L

L

A

I

G

G

F

V

I

L

T

L

S

N

L

Y

V

L

G

I

D

P

G

E

A

N

A

V

Y

F

E

L

F

L

L

I

L

A

T

S

L

I

S

A

A

T

L

F

A

A

G

T

F

V

I

W

T

V

W

W

A

L

G

M

I

I

S

L

W

L

C

T

H

Q

W

V

R

A

F

M

R

R

M

R

A

S

L

M

K

S

A

R

Q

-

G

-

Q

E

P

Q

L

V

T

A

D

Q

L

L

P

K

Y

F

R

P

A

V

R

P

F

F

F

W

P

P

A

Y

G

G

A

P

I

A

V

M

A

A

L

I

I

A

A

F

C

M

I

V

A

F

I

I

I

F

I

G

G

V

Q

L

A

G

Y

Y

G

F

P

P

V

D

T

T

S

Q

G

A

K

A

S

L

L

I

G

V

E

G

I

V

L

I

M

W

P

V

Y

V

I

F

G

L

I

V

P

A

V

S

F

Y

L

L

A

-

L

L

W

W

W

C

G

K

H

P

K

R

L

A

V

G

T

Q

R

G

A

Q

P

P

T

V

V

A

D

E

P

P

A

-

T

-

A

A

D

E

L

L

E

H

R

R

T27 (= T42) mutation T->A,S: Retains 60% of wild-type activity.; mutation to N: Retains 20% of wild-type activity.
E98 (≠ N114) mutation to A: Retains 80% of wild-type activity.
K156 (= K172) mutation K->A,Q: Retains less than 10% of wild-type activity.; mutation to R: Retains 40% of wild-type activity.
F212 (≠ Y224) mutation F->A,Q: Loss of activity.; mutation to Y: No change in activity.
E218 (= E230) mutation E->A,Q: Loss of activity.; mutation to D: Retains 70% of wild-type activity.

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
26% identity, 92% coverage: 21:475/493 of query aligns to 276:783/852 of Q03770

query
sites
Q03770

E

H

L

I

R

Q

R

R

S

K

L

L

L

K

S

V

R

R

H

H

L

I

T

Q

M

M

I

L

S

S

I

I

G

G

G

A

A

C

I

F

G

S

T

V

G

G

L

L

F

F

V

L

A

T

S

S

G

G

A

K

T

A

I

F

S

S

Q

I

A

A

G

G

P

P

G

F

G

G

A

T

L

L

V

L

A

G

Y

F

A

G

A

L

V

T

G

G

I

S

M

I

V

I

W

L

L

A

I

T

M

M

Q

L

S

S

L

F

G

T

E

E

M

L

A

S

A

T

Y

L

L

I

P

P

V

V

A

S

G

S

S

G

F

F

G

S

E

G

Y

L

G

A

T

S

R

R

F

F

V

V

S

E

P

D

S

A

F

F

G

G

F

F

A

A

I

L

G

G

W

W

N

T

Y

Y

W

W

F

I

N

S

W

C

A

M

I

L

T

A

V

L

A

P

A

A

E

Q

L

V

V

S

A

S

A
x
T

L

F

V

Y

M

L

K

S

Y

Y

W

Y

L

N

P

N

D

V

V

N

P

I

S

S

L

K

V

G

W

V

S

T

A

A

L

G

F

F

L

I

V

T

I

L

L

F

-

S

-

A

-

F

-

S

F

I

T

V

I

V

N

N

A

L

L

L

S

D

A

V

R

S

A

I

Y

M

G

G

E

E

S

I

E

V

F

Y

L

V

F

A

A

G

S

I

I

S

K

K

V

V

V

I

A

I

V

A

I

I

V

L

F

M

L

V

I

F

L

T

G

M

V

I

A

I

M

L

I

N

A

A

G

G

-

H

-

G

-

N

-

D

I

I

L

H

G

E

G

G

-

V

-

G

-

F

-

R

-

Y

-

W

-

D

P

S

S

S

P

K

G

S

T

V

E

R

N

N

W

L

T

T

-

Y

-

G

-

L

-

Y

-

R

-

P

-

T

-

F

-

D

-

L

-

A

-

D

-

A

-

G

T

E

G

G

E

S

A

K

P

K

F

G

V

I

G

S

G

G

G

P

E

K

G

G

I

R

L

F

L

L

-

A

-

T

-

A

-

S

V

V

L

M

L

L

V

I

A

S

G

T

Y

F

S

A

F

F

Q

S

G

G

T

V

E

E

L

M

I

T

G

F

T

L

A

A

A

S

G

G

E

E

A

A

E

I

N

N

P

P

E

R

R

K

T

T

I

I

P

P

R

S

A

A

I

T

R

K

T

R

I

T

F

F

W

S

R

I

I

V

L

L

L

I

F

S

Y

Y

I

V

G

F

A

L

I

I

A

F

V

S

I

V

G

G

F

I

L

N

I

I

P

Y

Y

S

T

G

D

D

P

P

N

R

L

L

N

S

-

Y

-

F

-

P

-

G

-

I

-

S

-

E

-

K

-

R

-

Y

-

E

-

A

-

I

-

K

-

G

S

T

A

G

E

M

D

D

-

W

-

R

-

L

-

R

-

T

-

N

-

C

-

R

-

G

-

I

-

D

-

Y

-

R

N

Q

V

I

S

S

V

V

-

G

-

T

-

G

-

Y

-

S

S

S

P

P

F

W

T

V

L

V

V

A

F

L

E

Q

R

N

A

F

G

G

I

L

L

C

G

T

A

F

A

A

S

S

V

A

I

F

N

N

A

A

I

I

I

L

L

I

T

F

S

F

V

T

L

A

S

T

A

A

G

G

T

I

S

S

G

S

L

L

Y

F

S

S

S

C

T

S

R

R

M

T

L

L

F

Y

A

A

L

M

A

S

E

V

R

Q

G

R

H

K

A

A

P

P

R

P

F

V

L

F

T

E

R

I

L

C

S

S

S

K

H

R

Q

G

V

V

P

P

M

Y

N

V

A

S

L

V

V

I

A

F

T

S

L

L

V

F

G

S

L

V

A

I

G

A

F

Y

I

I

T

A

S

V

L

D

V

Q

G

T

D

A

G

I

A

E

A

N

Y

F

E

D

F

V

L

L

L

A

T

N

L

V

S

S

A

S

L

A

A

S

G

T

F

S

I

I

T

I

W

W

A

M

G

G

I

L

S

N

W

L

C

S

H

F

W

L

R

R

F

F

R

Y

M

Y

A

A

L

L

K

K

A

Q

Q

R

G

K

Q

D

P

I

L

I

-

S

-

R

-

N

-

D

T

S

D

S

L

Y

P

P

Y

Y

R

K

A

S

R

P

F

F

F

Q

P

P

A

Y

G

L

A

A

I

I

V

Y

A

G

L

L

I

V

A

G

C

C

I

S

A

L

I

F

I

V

I

I

G

-

Q

-

A

-

Y

-

G

-

P

-

V

-

T

-

S

-

G

-

K

-

S

-

L

-

G

-

E

-

I

-

L

-

M

-

P

-

Y

F

I

M

G

G

I

Y

P

P

V

N

F

F

L

I

A

H

L

H

W

F

W

W

G

S

H

T

K

K

T382 (≠ A127) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
25% identity, 48% coverage: 16:250/493 of query aligns to 12:242/461 of P76037

query
sites
P76037

Q

Q

S

P

E

G

P

K

Q

T

E

R

L

L

R

R

R

K

S

S

L

L

L

K

S

L

R

W

H

Q

L

V

T

V

M

M

I

M

S

G

I

L

G

A

G

Y

A

L

I

T

G

P

T

M

G

T

L

V

F

F

-

D

-

T

-

F

-

G

V

I

A

V

S

S

G

G

A

I

T

S

I

D

S

G

Q

H

A

V

G

-

P

P

G

A

G

S

A

Y

L

L

V

L

A

A

Y

L

A

A

A

-

V

-

G

G

I

V

M

L

V

F

W

T

L

A

I

I

M

-

Q

-

S

S

L

Y

G

G

E

K

M

L

A

V

A

R

Y

Q

L

F

P

P

V

E

A

A

G

G

S

S

F

A

G

Y

E

T

Y

Y

G

A

T

Q

R

K

F

S

V

I

S

N

P

P

S

H

F

V

G

G

F

F

A

M

I

V

G

G

W

W

N

S

Y

S

W

L

F

L

N

D

W
x
Y

A

L

I

F

T

L

V

P

A

M

A

I

E

N

L

V

V

L

A

L

A

A

A

K

L

I

V

Y

M

L

K

S

Y

A

W

L

L

F

P

P

D

E

V

V

P

P

S

P

L

W

V

V

W

W

S

V

A

V

L

T

F

F

L

V

V

A

I

I

L

L

F

T

T

A

I

A

N

N

A

L

L

K

S

S

A

V

R

N

A

L

Y

V

G

A

E

N

S

F

E

N

F

T

L

L

F

F

A

V

S

L

I

V

K

Q

V

I

V

S

A

I

V

M

I

V

V

V

F

F

L

I

I

F

L

L

G

-

V

-

A

-

M

V

I

V

A

Q

G

G

I

L

L

H

G

K

G

G

P

E

S

G

P

V

G

G

T

T

E

V

N

-

W

W

T

S

T

L

G

-

E

-

A

Q

P

P

F

F

V

I

G

S

G

E

G

N

E

A

G

H

I

L

L

I

L

P

V

I

L

I

-

T

-

G

-

A

L

T

V

I

A

V

G

C

Y

F

S

S

F

F

Q

L

G

G

T

F

E

D

L

A

I

V

G

T

T

T

A

L

A

S

G

E

E

E

A

T

E

P

N

D

P

A

E

A

R

R

T

V

I

I

P

P

R

K

A

A

I

I

Y110 (≠ W115) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.

P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
23% identity, 70% coverage: 18:360/493 of query aligns to 24:392/629 of P30825

query
sites
P30825

E

E

P

E

Q

T

E

R

L

L

R

S

R

R

S

C

L

L

L

N

S

T

R

F

H

D

L

L

T

V

M

A

I

L

S

G

I

V

G

G

G

S

A

T

I

L

G

G

T

A

G

G

L

V

F

Y

V

V

A

L

S

A

G

G

A

A

T

V

I

A

S

R

Q

E

A

N

G

A

P

G

G

P

G

A

A

I

L

V

V

I

A

S

Y

F

A

L

A

I

V

A

G

A

I

L

M

A

V

S

W

V

L

L

I

A

M

G

Q

L

S

C

L

Y

G

G

E

E

M

F

A

G

A

A

Y

R

L

V

P

P

V

K

A

T

G

G

S

S

F

A

G

Y

E

L

Y

Y

G

S

T

Y

R

V

F

T

V

V

S

G

P

E

S

L

F

W

G

A

F

F

A

I

I

T

G

G

W

W

N

N

Y

L

W

I

F

L

N

S

W

Y

A

I

I

I

T

G

V

T

A

S

E

-

L

-

V

-

A

-

L

-

V

V

M

A

K

R

Y

A

W

W

L

S

P

A

D

T

V

F

P

D

S

E

L

L

V

I

W

G

S

R

A

P

L

I

-

G

-

E

F

F

L

S

V

R

I

T

L

H

F

M

T

T

I

L

N

N

A

A

L

P

S

G

A

V

R

L

A

A

Y

-

G

-

E

E

S

N

E

P

F

D

L

I

F

F

A

A

S

V

I

I

-

I

-

L

-

I

-

L

-

T

-

G

-

L

-

T

-

L

-

G

-

V

-

K

-

E

-

S

-

A

-

M

-

V

-

N

K

K

V

I

V

F

A

T

V

C

I

I

V

N

F

V

L

L

I

V

L

L

G

G

V

F

A

I

M

M

I

V

A

S

G

G

I

F

L

V

G

K

G

G

P

-

S

-

P

-

G

S

T

V

E

K

N

N

W

W

T

Q

T

L

G

T

E

E

A

E

P

D

F

F

-

G

-
x
N

-

T

-

S

-

G

-

R

-

L

-

C

-

L

-

N

-

D

-

T

-

K

-

E

-

G

-

K

-

P

-

G

V

V

G

G

-

G

-

F

-

M

-

P

-

F

G

G

G

F

E

S

G

G

I

V

L

L

L

S

V

G

L

A

V

T

A

C

G

F

Y

Y

S

A

F

F

Q

V

G

G

T

F

E

D

L

C

I

I

G

A

T

T

A

T

A

G

G

E

E

E

A

V

E

K

N

N

P

P

E

Q

R

K

T

A

I

I

P

P

R

V

A

G

I

I

R

V

T

A

I

S

F

L

W

L

R

I

I

C

L

F

L

I

F

A

Y

Y

I

F

G

G

A

V

I

S

A

A

V

A

I

L

G

T

F

L

L

M

I

M

P

P

Y

Y

T

F

D

-

P

-

N

-

L

-

N

-

S

C

A

L

E

D

D

N

N

N

V

-

S

-

V

-

S

S

P

P

F

L

T

P

L

D

V

A

F

F

E

K

R

H

A

V

G

G

I

W

L

E

G

G

A

A

A

K

S

Y

V

A

I

V

N

A

A

V

I

G

I

S

L

L

T

C

S

A

V

L

L

S

S

A

A

S

G

L

T

L

S

G

G

S

L

M

Y

F

S

P

S

M

T

P

R

R

M

V

L

I

F

Y

A

A

L

M

A

A

E

E

R

D

G

G

H

L

A

L

P

F

R

K

F

F

L

L

T

A

R

N

L

V

S

N

S

D

H

R

-

T

Q

K

V

T

P

P

M

I

N

I

A

A

L

T

V

L

A

A

T

S

N226 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine

6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
27% identity, 84% coverage: 21:436/493 of query aligns to 22:421/458 of 6f34A

query
sites
6f34A

E

S

L

L

R

R

R

K

S

E

L

L

L
x
G

S

A

R
x
F

H
x
D

L

L

T

T

M
|
M

I

L

S

G

I

I

G

G

G

A

A
x
I

I

I

G
|
G

T
|
T

G
|
G

L

I

F

F

V

V

A

L

S

T

G

G

-

V

A

A

T

A

I

A

S

E

Q

H

A

A

G

G

P

P

G

A

G

L

A

V

L

L

V

-

A

S

Y

F

A

I

A

L

V

S

G

G

I

L

M

A

V

C

W

V

L

F

I

A

M

A

Q

L

S

C

L

Y

G

A

E

E

M

F

A

A

A

S

Y

T

L

V

P

P

V

V

A

S

G

G

S

S

-

A

-

Y

-

T

-

Y

-

S

-

Y

-

A

-

T

F

F

G

G

-

E

-

L

-

I

-

A

-

W

-

I

-

L

-

G

-

W

-

D

-

L

-

I

-

L

E
|
E

Y
|
Y

G

G

T

V

R
x
A

F

-

V

-

S

-

P

-

S

S

F

S

G

A

F

V

A

A

I

V

G

G

W

W

N

S

Y

G

W

Y

F

F

N

Q

W

G

A

L

I

L

T

S

-

G

-

E

V

L

A

P

A

K

E

A

L

L

V

T

A

S

A

A

A

Y

L

D

V

P

M

A

K

K

Y

G

W

T

L

F

P

I

D

D

V

L

P

P

S

A

L

I

V

I

W

-

S

-

A

-

L

I

F

V

L

L

V

F

I

I

L

T

F

F

T

L

I

L

N

N

A

-

L

L

S

G

A

A

R

K

A

K

Y

S

G
x
A

E
x
R

S

F

E

N

F

A

L

V

F

I

A

V

S
x
A

I
|
I

K

K

V

V

V
x
A

A

V

V

V

I

L

V
x
L

F

F

L

L

I

A

L

V

G

G

V

V

A
x
W

M
x
Y

I

V

A

K

G

-

I

-

L

-

G

-

P

-

S

-

P

-

G

-

T

P

E

E

N

N

W

W

T

T

T

-

G

-

E

-

A

-

P

P

F

F

V

M

G

P

G

Y

G

G

-

F

E

S

G

G

I

V

L

A

L

T

V

G

L

A

V

T

A

V

G

F

Y
x
F

S
x
A

F

Y

Q
x
I

G

G

T

F

E

D

L

A

I

V

G

S

T

T

A

A

G

E

E

E

A

V

E

R

N

N

P

P

E

Q

R

R

T

D

I

M

P

P

R

I

A

G

I

I

R

I

T

V

I

S

F

L

W

L

R

V

I

C

L

T

L

L

F

L

Y

Y

I

I

G

A

A

V

I

S

A

L

V

V

I

L

G

T

F

G

L

I

I

V

P

P

Y

Y

T

E

D

Q

P

L

N

N

L

V

L

K

N

N

S

P

A

V

E

A

D

F

N

A

V

L

S

N

V

Y

S

I

P

H

F
x
Q

T

D

L

W

V
|
V

F

-

E

-

R

-

A

A

G

G

I

F

-

I

-

S

L

L

G

G

A

A

A

I

S

A

V

G

I

I

N

T

A

T

I
x
V

I

L

L

L

T

V

S

S

V

-

L

-

S

-

A

-

G

-

T

-

S

-

G

-

L

M

Y

Y

S

G

S

Q

T

T

R

R

M

L

L

F

F

Y

A

A

L

I

A

S

E

R

R

D

G

G

H

L

A

L

P

P

R

K

F

V

L

F

T

A

R

R

L

I

S

S

-

P

S

T

H

R

Q

Q

V

V

P

P

M

Y

N

-

A

-

L

-

V

V

A

N

T

T

T

W

L

L

V

T

G

G

L

A

A

A

G

V

F

A

I

V

T

F

S

A

L

G

V

I

G

I

D

P

G

L

A

N

A

K

Y

L

E

A

F

E

L

L

L

T

T

N

L

I

S

G

A

T

L

L

A

F

G

A

F

F

I

I

T

T

W

V

A

S

G

-

I

-

S

-

W

-

C

-

H

-

W

-

R

-

F

I

R

G

M

V

A

L

L

V

K

L

A

R

Q

K

G

T

Q

Q

P

P

L

D

T

L

D

K

L

R

P

A

Y

F

R

R

A

V

R

P

F

F

F

V

P

P

A

V

G

V

A

P

I

I

V

L

A

A

L

V

I

L

A

F

C

C

binding arginine: I40 (≠ A39), G42 (= G41), T43 (= T42), G44 (= G43), E115 (= E93), Y116 (= Y94), A119 (≠ R97), F228 (≠ Y224), A229 (≠ S225), I231 (≠ Q227), V314 (≠ I311)
binding cholesterol: W201 (≠ A185), Y202 (≠ M186)
binding : G28 (≠ L27), F30 (≠ R29), D31 (≠ H30), M34 (= M33), A178 (≠ G162), R179 (≠ E163), A186 (≠ S170), I187 (= I171), A190 (≠ V174), L194 (≠ V178), Q296 (≠ F292), V299 (= V295)

6f2wA Bacterial asc transporter crystal structure in open to in conformation (see paper)
24% identity, 88% coverage: 34:467/493 of query aligns to 12:424/433 of 6f2wA

query
sites
6f2wA

I

V

S

V

I

V

G

G

G

T

A
x
V

I

I

G
|
G

T
x
A

G
|
G

L

I

F

F

V

F

A

K

S

P

G

T

A

A

T

V

I

Y

S

G

Q

A

A

A

G

G

-

A

P

P

G

G

G

L

A

G

L

L

V

L

A

A

Y

W

A

F

A

V

V

A

G

G

I

I

M

I

V

T

W

I

L

A

I

G

M

G

Q

L

S

T

L

V

G

A

E

E

M

I

A

G

A

T

Y

I

L

Y

P

P

V

Q

A

T

G

G

S

G

F

M

G

M

E

I

Y

Y

G

L

T

E

R

K

F

V

V

Y

S

G

P

R

S

W

F

L

G

G

F

F

A

L

I

V

G

G

W

W

N

A

Y

Q

-

M

-

V

-

I

W

Y

F

Y

N

P

W

A

A

N

I

I

T

A

V

A

A

L

A

A

E

I

L

I

V

F

A

A

A

T

A

Q

L

F

V

V

M

N

K

L

Y

F

W

A

L

L

P

S

D

D

-

S

-

T

-

I

V

V

P

P

S

T

L

A

V

I

W

L

S

T

A

S

L

I

F

F

L

L

V

M

I

-

L

-

F

-

T

G

I

V

N

N

A

F

L

L

S

G

A

T

R

K

A

Y

Y

S

G

G

E

W

S

I

E

Q

F

T

L

L

F

A

A

T

S

I

I

L

K

K

V

L

V

I

A

P

V

L

I

V

V

V

F

I

L

I

I

V

L

-

G

-

V

-

A

-

M

-

I

-

A

A

G

G

I

L

L

L

-

Y

-

P

G

G

P

G

S

V

P

I

G

R

T

L

E

V

N

P

W

F

T

S

T

V

G

E

E

T

A

H

P

P

F

V

V

L

G

T

G

S

G

F

E

G

G

S

I

A

L

L

L

I

V

A

L

T

L

L

V

-

A

-

G

-

Y
x
F

S
x
A

F

Y

Q
x
D

G

G

T

W

E

I

L

N

I

V

G

G

T

T

A

L

A

A

G

G

E

E

A

M

E

K

N

N

P

P

E

G

R

K

T

M

I

L

P

P

R

K

A

V

I

I

R

-

T

-

I

-

F

-

W

-

R

-

I

-

L

-

F

-

Y

-

I

I

G

G

A

G

I

L

A

S

V

I

I

V

G

-

F

-

L

M

I

A

P

V

Y

Y

T

L

D

L

P

T

N

N

L

I

-

A

-

Y

-

L

-

F

-

V

L

L

N

D

S

S

A

S

E

Q

D

L

N

A

V

G

S

T

V

D

S

T

P

P

F

A

T

A

L

L

V

V

-

A

-

S

-

H

-

L

F

F

E

E

R

G

A

I

G

G

I

-

L

-

G

-

A

-

A

S

S

K

V

L

I

V

N

T

A

I

I

G

I

I

L

L

T

I

S

S

V

V

L

F

S

G

A

G

G

I

T

N

S

G

G

Y

L

I

Y

I

S

S

S

G

T

L

R

R

M

V

L

P

F

Y

A

A

L

L

A

A

E

T

R

Q

G

K

H

M

A

L

P

P

-

F

-

S

R

D

F

W

L

F

T

A

R

R

L

I

S

N

S

P

H

K

-

T

Q

N

V

L

P

P

M

I

N

N

A

G

L

-

V

-

A

-

T

-

L

-

V

-

G

-

L

-

A

-

G

-

F

-

I

-

T

-

S

G

L

L

V

V

G

M

D

L

G

G

A

I

A

A

Y

I

E

V

F

M

L

I

L

L

T

T

-

G

-

Q

-

F

-

N

-

Q

L

L

S

T

A

D

L

L

A

I

G

V

F

F

I

V

T

I

W

W

A

F

G

F

I

I

S

T

W

L

C

T

H

F

W

-

R

-

F

I

R

A

M

V

A

I

L

I

K

L

A

R

Q

K

G

T

Q

Q

P

P

L

D

T

I

D

E

L

R

P

P

Y

Y

R

R

A

V

R

P

F

F

F

Y

P

P

A

V

G

I

A

P

I

L

V

I

A

A

L

I

I

I

A

G

C

G

I

L

A

Y

I

I

I

F

G

N

Q

T

A

L

Y

I

G

-

P

-

V

V

T

Q

S

P

G

K

K

N

S

A

L

F

G

I

E

G

I

I

L

L

M

L

P

T

Y

L

I

I

G

G

I

I

P

P

V

I

F

Y

binding alpha-aminoisobutyric acid: V17 (≠ A39), G19 (= G41), A20 (≠ T42), G21 (= G43), F199 (≠ Y224), A200 (≠ S225), D202 (≠ Q227)

5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
27% identity, 84% coverage: 21:436/493 of query aligns to 20:419/456 of 5oqtA

query
sites
5oqtA

E

S

L

L

R

R

R

K

S
x
E

L

L

L
x
G

S

A

R
x
F

H
x
D

L

L

T

T

M
|
M

I

L

S

G

I

I

G

G

G

A

A
x
I

I

I

G
|
G

T
|
T

G
|
G

L

I

F

F

V

V

A

L

S

T

G

G

-

V

A

A

T

A

I

A

S

E

Q

H

A

A

G

G

P

P

G

A

G

L

A

V

L

L

V

-

A

S

Y

F

A

I

A

L

V

S

G

G

I

L

M

A

V

C

W

V

L

F

I

A

M

A

Q

L

S

C

L

Y

G

A

E

E

M

F

A

A

A

S

Y

T

L

V

P

P

V

V

A

S

G

G

S

S

-

A

-

Y

-

T

-

Y

-

S

-

Y

-

A

-

T

F

F

G

G

-

E

-

L

-

I

-

A

-

W

-

I

-

L

-

G

-

W

-

D

-

L

-

I

-

L

E

E

Y

Y

G

G

T

V

R

A

F

-

V

-

S

-

P

-

S

S

F

S

G

A

F

V

A

A

I

V

G

G

W

W

N

S

Y

G

W

Y

F

F

N

Q

W

G

A

L

I

L

T

S

-

G

-

E

V

L

A

P

A

K

E

A

L

L

V

T

A

S

A

A

A

Y

L

D

V

P

M

A

K

K

Y

G

W

T

L

F

P

I

D

D

V

L

P

P

S

A

L

I

V

I

W

-

S

-

A

-

L

I

F

V

L

L

V

F

I

I

L

T

F

F

T

L

I

L

N

N

A

-

L

L

S

G

A

A

R

K

A

K

Y

S

G
x
A

E
x
R

S

F

E

N

F

A

L

V

F

I

A

V

S
x
A

I

I

K

K

V

V

V
x
A

A

V

V

V

I

L

V
x
L

F

F

L

L

I

A

L

V

G

G

V

V

A

W

M

Y

I

V

A

K

G

-

I

-

L

-

G

-

P

-

S

-

P

-

G

-

T

P

E

E

N

N

W

W

T

T

T

-

G

-

E

-

A

-

P

P

F

F

V

M

G

P

G

Y

G

G

-

F

E

S

G

G

I

V

L

A

L

T

V

G

L

A

V

T

A

V

G

F

Y
x
F

S
x
A

F

Y

Q
x
I

G

G

T

F

E

D

L

A

I

V

G

S

T

T

A

A

G

E

E

E

A

V

E

R

N

N

P

P

E

Q

R

R

T

D

I

M

P

P

R

I

A

G

I

I

R

I

T

V

I

S

F

L

W

L

R

V

I

C

L

T

L

L

F

L

Y

Y

I

I

G

A

A

V

I

S

A

L

V

V

I

L

G

T

F

G

L

I

I

V

P

P

Y

Y

T

E

D

Q

P

L

N

N

L

V

L

K

N

N

S

P

A

V

E

A

D

F

N

A

V

L

S

N

V

Y

S

I

P

H

F
x
Q

T

D

L

W

V
|
V

F

-

E

-

R

-

A

A

G

G

I

F

-

I

-

S

L

L

G

G

A

A

A

I

S

A

V

G

I

I

N

T

A

T

I

V

I

L

L

L

T

V

S

M

V

-

L

-

S

-

A

-

G

-

T

-

S

-

G

-

L

M

Y

Y

S

G

S

Q

T

T

R

R

M

L

L

F

F

Y

A

A

L

I

A

S

E

R

R

D

G

G

H

L

A

L

P

P

R

K

F

V

L

F

T

A

R

R

L

I

S

S

-

P

S

T

H

R

Q

Q

V

V

P

P

M

Y

N

-

A

-

L

-

V

V

A

N

T

T

T

W

L

L

V

T

G

G

L

A

A

A

G

V

F

A

I

V

T

F

S

A

L

G

V

I

G

I

D

P

G

L

A

N

A

K

Y

L

E

A

F

E

L

L

L

T

T

N

L

I

S

G

A

T

L

L

A

F

G

A

F

F

I

I

T

T

W

V

A

S

G

-

I

-

S

-

W

-

C

-

H

-

W

-

R

-

F

I

R

G

M

V

A

L

L

V

K

L

A

R

Q

K

G

T

Q

Q

P

P

L

D

T

L

D

K

L

R

P

A

Y

F

R

R

A

V

R

P

F

F

F

V

P

P

A

V

G

V

A

P

I

I

V

L

A

A

L

V

I

L

A

F

C

C

binding alanine: I38 (≠ A39), G40 (= G41), T41 (= T42), G42 (= G43), F226 (≠ Y224), A227 (≠ S225), I229 (≠ Q227)
binding : E24 (≠ S25), G26 (≠ L27), F28 (≠ R29), D29 (≠ H30), M32 (= M33), A176 (≠ G162), R177 (≠ E163), A184 (≠ S170), A188 (≠ V174), L192 (≠ V178), Q294 (≠ F292), V297 (= V295)

Q9QXW9 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; mLAT2; Solute carrier family 7 member 8 from Mus musculus (Mouse) (see paper)
24% identity, 90% coverage: 34:475/493 of query aligns to 47:471/531 of Q9QXW9

query
sites
Q9QXW9

I

I

S

I

I

V

G

G

G

N

A

I

I

I

G

G

T

S

G

G

L

I

F

F

V

V

A

S

S

P

G

K

A

G

T

V

I

L

S

E

Q

N

A

A

G

G

P

S

G

V

G

G

-

L

A

A

L

L

V

I

A

V

Y

W

A

I

A

V

V

T

G

G

I

I

M

I

V

T

W

A

L

V

I

G

M

A

Q

L

S

C

L

Y

G

A

E

E

M

L

A

G

A

V

Y

T

L

I

P

P

V

K

A

S

G

G

S

-

F

-

G

G

E

D

Y

Y

G

-

T

-

R

S

F

Y

V

V

S

K

P

D

S

I

F

F

G

G

F

G

A

L

I

A

G

G

W

F

-

L

N

R

Y

L

W

W

F

I

N

A

W

V

A

L

I

V

T

I

V
x
Y

A

P

A

T

E
x
N

L

Q

V

A

A

V

A

I

A

A

L

L

V

T

M

F

K

S

Y

N

W

Y

L

V

-

L

-

Q

-

P

-

L

-

F

-

P

-

T

-

C

-

F

-

P

P

P

D

E

V

S

P

G

S

L

L

R

V

L

W

L

S

A

A

A

L

I

F

C

L

L

V

L

I

L

L

L

F

T

T

W

I

V

N

N

A

C

L

S

S

S

A

V

R

R

A

W

Y

A

G

T

E

R

S

V

E

Q

F

D

L

I

F

F

A

T

S

A

I

G

K

K

V

L

A

A

V

L

I

A

V

L

F

I

L

I

I

I

L

M

G

G

V

I

A

V

M

Q

I

I

A

C

G

K

-

G

-

E

-

F

I

F

L

W

G

L

G

E

P

P

S

K

P

N

G

A

T

F

E

E

N

N

W

F

T

-

G

-

E

Q

A

E

P

P

F

D

V

I

G

G

G

-

E

-

G

L

I

V

L

A

L

L

V

A

L

F

L

L

V

Q

A

G

G

S

Y
x
F

S

A

F

Y

Q

G

G

G

T

W

E

N

L

F

I

L

G

N

T

Y

A

V

A

T

G

E

E

E

A

L

E

V

N

D

P

P

E

Y

R

K

T

N

I

L

P

P

R

R

A

A

I

-

R

-

T

-

I

I

F

F

W

I

R

S

I

I

-

P

L

L

L

V

F

T

Y

F

I

V

G

Y

A

V

I

F

A

A

V

N

I

I

G

A

F

Y

L

V

I

T

P

A

Y

M

T

S

D

P

P

Q

N

E

L

L

N

A

S

S

A

N

E

A

D

V

N

A

V

V

S

T

V

F

S

G

P

-

F

-

T

-

L

-

V

-

F

-

E

-

R

-

A

E

G

K

I

L

L

L

G

G

A

V

A

M

S

A

V

W

I

I

N

M

A

P

I

I

I

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L

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A

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L

V

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L

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A

G

G

G

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V

S

N

G

G

-

S

L

L

Y

F

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T

S

S

T

S

R

R

M

L

L

F

F

F

A

A

L

G

A

A

E

R

R

E

G

G

H

H

A

L

P

P

R

S

F

V

L

L

T

A

R

M

L

I

S

H

S

V

H

K

Q

R

V

C

-

T

P

P

M

I

N

P

A

A

L

L

V

L

A

F

T

T

T

C

L

L

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S

G

T

L

L

A

L

G

M

F

L

I

V

T

T

S

S

L

D

V

-

G

-

D

-

G

-

A

-

Y

-

E

-

F

-

L

M

L

Y

T

T

L

L

S

I

A

N

L

Y

A

V

G

G

F

F

I

I

T

N

W

Y

-

L

-

F

-

Y

-

G

-

V

-

T

-

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A

A

G

G

I

Q

S

I

W

V

C

L

H

R

W

W

R

K

F

-

R

-

M

-

A

-

L

-

K

-

A

-

Q

-

G

-

Q

K

P

P

L

D

T

I

D

P

L

R

P

P

Y

I

R

K

A

V

R

S

-

L

F

L

F

F

P

P

A

-

G

-

A

I

I

I

V

Y

A

L

L

L

I

F

A

W

C

A

I

F

A

L

I

L

I

I

I

F

G

S

Q

L

A

W

Y

S

G

E

P

P

V

V

T

V

S

C

G

G

K

I

S

G

L

L

G

A

E

I

I

M

L

L

M

T

P

-

Y

-

I

-

G

G

I

V

P

P

V

V

-

Y

F

F

L

L

A

G

L

V

W

Y

W

W

G

Q

H

H

K

K

Y130 (≠ V119) mutation to A: Increases T2 import. Increases T3 and enables T4 import. Does not affect L-leucine and L-phenylalanine uptake.
N133 (≠ E122) mutation to S: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake. Increases the export of both L-leucine and L-phenylalanine.
F242 (≠ Y224) mutation to W: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake.

Q22397 Amino acid transporter protein 6 from Caenorhabditis elegans (see paper)
22% identity, 82% coverage: 36:441/493 of query aligns to 30:421/523 of Q22397

query
sites
Q22397

I

V

G

G

G

N

A

I

I

V

G

G

T

S

G

G

L

I

F

F

V

I

A

T

S

P

G

T

A

S

T

I

I

I

S

E

Q

N

A

V

G

N

P

S

G

V

G

G

A

-

L

-

V

-

A

-

Y

-

A

-

V

L

G

S

I

L

M

A

V

I

W

W

L

I

I

-

M

-

Q

-

S

-

L

-

G

-

E

-

M

L

A

A

Y

F

L

I

P

S

V

M

A

L

G

G

S

S

F

F

G

C

-

Y

-

V

E

E

Y

L

G

G

T

T

-

S

-

I

R

R

F

L

V

S

S

G

P

G

S

D

F

F

G

A

F

Y

A

-

I

L

G

C

W

F

N

M

Y

K

W

W

F

Y

-

P

-

V

-

A

-

F

-

A

-

F

-

M

-

C

-

I

-

G

-

C

-

T

-

I

N

N

W

Y

A

P

I

A

T

T

V

L

A

A

A

-

E

-

L

-

V

V

A

Q

A

A

A

Q

L

T

V

F

M

A

K

E

Y

Y

W

V

L

F

P

R

-

G

-

A

-

G

-

V

-

E

-

L

D

D

V

E

P

T

S

S

L

E

V

F

W

W

S

A

A

K

L

K

F

L

L

L

-

G

-

F

-

S

-

L

-

I

V

I

I

L

L

L

F

M

T

F

I

M

N

N

A

F

L

F

S

S

A

L

R

K

A

T

Y

F

G

V

E

Q

S

R

E

F

F

S

L

I

F

L

A

A

S

S

I

L

-

A

K

K

V

I

V

A

A

A

V

T

I

L

V

L

F

I

L

I

I

I

L

T

G

G

V

F

A

Y

M

Y

I

L

-

I

-

F

-

K

-

H

-

W

A

K

G

Q

I

N

L

L

G

E

P

P

S

F

P

K

G

G

T

S

E

-

N

N

W

W

T

N

T

P

G

G

E

-

A

-

P

P

F

F

V

V

G

N

G

A

G

-

E

-

G

-

I

-

L

-

V

-

L

-

L

L

V

F

A

A

G

G

-

L

Y

F

S

S

F

Y

Q

D

G

G

T

W

E

D

L

I

I

L

G

N

T

F

A

G

A

A

G

E

E

E

A

I

E

E

N

N

P

P

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K

R

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A

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I

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T

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I

-

F

-

W

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I

I

I

L

G

L

M

F

T

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C

I

I

G

G

A

V

I

I

A

Y

V

V

I

-

G

A

F

V

L

N

I

V

P

A

Y

Y

T

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D

-

P

-

N

-

L

I

L

V

N

L

S

S

A

P

E

T

D

E

N

M

V

I

S

A

V

S

S

N

P

A

F

V

T

A

L

I

V

D

F

F

E

A

R

N

A

K

G

T

I

L

L

G

G

A

A

A

S

F

V

V

I

V

N

P

A

V

I

M

I

V

L

A

T

I

S

L

V

L

L

I

S

G

A

S

G

L

T

N

S

S

G

T

L

M

Y

F

S

S

S

A

T

S

R

R

M

Y

L

L

F

Q

A

A

L

V

A

S

E

R

R

Q

G

G

H

H

A

I

P

P

R

S

F

A

L

I

T

S

R

G

L

I

S

A

S

P

H

N

-

C

Q

D

V

S

P

P

M

R

N

V

A

A

L

L

V

L

A

V

T

H

T

I

L

L

V

I

G

A

L

I

A

A

G

-

F

-

I

-

T

V

S

S

L

F

V

L

G

G

D

D

G

P

A

-

Y

-

E

-

F

-

L

-

T

-

L

-

S

D

A

K

L

L

A

I

G

N

F

Y

I

V

T

A

W

F

A

A

G

-

I

-

S

Q

W

W

C

S

H

Q

W

R

R

A

F

F

R

T

M

M

A

S

-

A

-

L

-

Y

L

L

K

R

A

I

Q

R

G

G

Q

R

P

P

L

R

T

H

D

-

L

-

P

P

Y

D

R

R

A

I

R

Q

F

L

F

P

P

I

A

I

G

M

A

P

I

I

V

L

A

F

L

F

I

L

A

V

C

C

I

T

A

S

I

M

I

V

Sites not aligning to the query:

521:523 PDZ-binding motif; mutation Missing: Abolishes the interaction with nrfl-1.

O34739 Serine/threonine exchanger SteT from Bacillus subtilis (strain 168) (see paper)
24% identity, 69% coverage: 16:353/493 of query aligns to 2:323/438 of O34739

query
sites
O34739

Q

H

S

T

E

E

P

D

Q

N

E

G

L

L

R

K

S

E

L

I

-

G

L

L

S

L

R

F

H

A

L

L

T

T

M

L

I

V

S

-

I

I

G

G

G

T

A

I

I

I

G

G

T

S

G

G

L

V

F

F

V

M

A

K

S

P

G

G

A

A

T

V

I

L

S

A

Q

Y

A

S

G

G

P

D

G

S

G

K

-

M

A

A

L

L

V

F

A

A

Y

W

A

L

V

G

G

G

I

I

M

L

V

T

W

L

L

A

I

G

M

G

Q

L

S

T

L

I

G

A

E

E

M

I

A

G

A

T

Y

Q

L

I

P

P

V

K

A

T

G

G

S

G

F

L

G

Y

E

T

Y

Y

G

L

T

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R

E

F

V

V

Y

S

G

P

E

S

F

F

W

G

G

F

F

A

L

I
x
C

G

G

W

W

N

V

Y

Q

W

I

F

I

N

I

W

Y

-

G

-

P

A

A

I

I

T

I

V

G

A

A

A

L

E

G

L

L

V

Y

A

F

A

G

A

S

L

L

V

M

M

A

K

N

Y

L

W

F

-

G

-

W

-

G

-

S

-

G

L

L

P

S

D

K

V

V

P

I

S

G

L

I

V

I

W

-

S

A

A

V

L

L

F

F

L

L

V
x
C

I

V

L

-

F

-

T

-

I

I

N

N

A

I

L

I

S

G

A

T

R

K

A

Y

Y

G

G

G

E

F

S

V

E

Q

F

T

L

L

F

T

A

T

S

I

I

G

K

K

V

L

V

I

A

P

V

I

I

A

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x
C

F

I

L

I

I

V

L

F

G

G

V

L

A

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M

-

I

-

A

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G

-

I

-

L

-

G

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P

-

S

-

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-

G

-

T

-

E

-

N

-

W

W

T

K

T

G

G

D

E

Q

A

H

P

I

F

F

V

T

G

A

G

V

G

N

E

E

G

S

I

I

L

S

L

D

V

M

-

N

-

F

-

G

-

A

L

I

L

L

V

A

A

T

G

L

Y

F

S

A

F

Y

Q

D

G

G

T

W

E

I

L

L

I

L

G

A

T

A

A

L

A

G

G

G

E

E

A

M

E

K

N

N

P

P

E

E

R

K

T

L

I

L

P

P

R

R

A

A

I

M

R

T

T

G

I

G

F

L

W

L

R

I

I

V

L

T

L

A

F

I

Y

Y

I

I

G

-

A

-

I

-

A

-

V

F

I

I

G

N

F

F

-

A

L

L

I

L

P

H

Y

I

T

L

D

S

P

A

N

N

L

E

L

I

N

V

S

T

A

L

E

G

D

E

N

N

V

A

S

T

V

S

S

T

P

A

F

A

T

T

L

M

V

L

F

F

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G

R

S

A

I

G

G

I

-

L

-

G

-

A

-

A

G

S

K

V

L

I

I

N

S

A

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I

G

I

I

L

I

T

V

S

S

V

I

L

F

S

G

A
x
C

G

L

T

N

S

G

G

K

L

V

Y

L

S

S

S

F

T

P

R

R

M

V

L

S

F

F

A

A

L

M

A

A

E

E

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R

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K

H

Q

A

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P

R

-

F

F

L

A

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L

S

-

S

S

H

H

Q

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H

P

P

C94 (≠ I107) mutation to S: Retains 25% of the transport activity; when associated with S-141; S-168; S-291 and S-415.
C141 (≠ V148) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-168; S-291 and S-415.
C168 (≠ V178) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-291 and S-415.
C291 (≠ A319) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-415.

Sites not aligning to the query:

415 C→S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-291.

6li9B Heteromeric amino acid transporter b0,+at-rbat complex bound with arginine (see paper)
22% identity, 69% coverage: 34:374/493 of query aligns to 9:347/458 of 6li9B

query
sites
6li9B

I

I

S

I

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V

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G

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A
x
I

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|
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x
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|
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F

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A

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A

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A

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S

-

F

-

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G

E

E

Y

Y

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M

P

E

S

A

F

Y

G

G

F

P

A

I

I

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G

A

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-

N

-

Y

Y

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L

F

F

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W

A

A

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A

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-

I

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F

A

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A

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x
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A

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D

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A

I

A

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A

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-

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F

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M

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D

A

P

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E

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N

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N

A

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F

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P

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A

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A

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P

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-

I

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N

binding arginine: I14 (≠ A39), G16 (= G41), S17 (≠ T42), G18 (= G43), W201 (≠ Y224), A202 (≠ S225), D204 (≠ Q227)

Q9FFL1 Polyamine transporter RMV1; Protein RESISTANT TO METHYL VIOLOGEN 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
21% identity, 78% coverage: 51:437/493 of query aligns to 76:433/490 of Q9FFL1

query
sites
Q9FFL1

T

S

I

V

S

K

Q

A

A

A

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P

G

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A

A

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A

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F

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Q

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A

A

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A

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-

A

A

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-

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W

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-

L

-

I

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G

S

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A

L

A

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G

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E

A

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E

E

N

N

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K

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A

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R

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-

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L

F

F

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Y

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A

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A

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V

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G

S

F

Y

L

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I

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P

-

V

Y

L

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D

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P

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N

G

L

A

L

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N

A

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L

A

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D

K

N

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A

I

G

G

I

G

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A

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A

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W

V

W

I

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A

A

I

A

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A

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T

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S

V

N

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M

S

G

A

M

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A

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Y

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S

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T

S

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F

M

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L

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L

A

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M

A

A

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R

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G

H

M

A

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E

F

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F

T

A

R

K

L

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S

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V

A

G

L

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L

-

F

-

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-

A

-

S

A

G

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I

L
x
I

V

L

G

S

L

W

A

L

G

S

F

F

I

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S

I

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A

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A

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L

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A

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F

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F

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G

-

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-

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-

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-

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F

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R

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M

M

A

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A

A

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A

G

S

Q

R

P

P

L

F

T

K

D

-

L

-

P

-

Y

-

R

-

A

-

R

-

F

-

F

I

P

P

A

V

G

G

A

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I

L

V

G

A

S

L

V

I

L

A

M

C

C

I

I

I377 (≠ L362) mutation to F: Loss of sensitivity to paraquat.

Query Sequence

>WP_017178492.1 NCBI__GCF_000295095.1:WP_017178492.1
MTDSTQAGPVAADGEQSEPQELRRSLLSRHLTMISIGGAIGTGLFVASGATISQAGPGGA
LVAYAAVGIMVWLIMQSLGEMAAYLPVAGSFGEYGTRFVSPSFGFAIGWNYWFNWAITVA
AELVAAALVMKYWLPDVPSLVWSALFLVILFTINALSARAYGESEFLFASIKVVAVIVFL
ILGVAMIAGILGGPSPGTENWTTGEAPFVGGGEGILLVLLVAGYSFQGTELIGTAAGEAE
NPERTIPRAIRTIFWRILLFYIGAIAVIGFLIPYTDPNLLNSAEDNVSVSPFTLVFERAG
ILGAASVINAIILTSVLSAGTSGLYSSTRMLFALAERGHAPRFLTRLSSHQVPMNALVAT
TLVGLAGFITSLVGDGAAYEFLLTLSALAGFITWAGISWCHWRFRMALKAQGQPLTDLPY
RARFFPAGAIVALIACIAIIIGQAYGPVTSGKSLGEILMPYIGIPVFLALWWGHKLVTRA
PTVDPATADLERK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory