SitesBLAST

G90 (= G64) to S: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908005
E104 (= E78) natural variant: Missing (in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs387906308)
152:161 (vs. 126:135, 60% identical) binding in other chain
R171 (= R145) to W: 69% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1800556
WIT 185:187 (≠ LIS 158:160) binding in other chain
A192 (≠ S165) to V: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940874
G209 (≠ R183) to S: 86% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1799958
R297 (= R271) binding FAD
Q308 (= Q282) binding in other chain
R325 (= R299) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908006
S353 (≠ I327) to L: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28941773
QILGG 365:369 (≠ EILGG 339:343) binding FAD
R380 (= R357) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940875
TSE 394:396 (≠ PPS 371:373) binding in other chain

Sites not aligning to the query:

1:24 modified: transit peptide, Mitochondrion

7y0aC Crystal structure of human short-chain acyl-coa dehydrogenase
32% identity, 96% coverage: 10:386/393 of query aligns to 12:385/387 of 7y0aC

query
sites
7y0aC

E

E

E

T

F

H

Q

Q

Q

M

Y

L

L

L

M

Q

E

T

I

C

R

R

E

D

L

F

A

A

Q

E

G

K

P

E

F

L

D

F

E

P

L

I

Q

A

T

A

E

Q

I

V

E

D

V

K

T

E

N

H

K

L

F

F

P

P

D

A

E

A

F

Q

Y

V

E

K

L

K

A

M

K

G

E

G

H

L

N

G

L

L

Y

L

R

A

F

M

Y

D

M

V

P

P

E

E

Q

E

Y

L

G

G

W

A

G

G

L

L

S

D

T

Y

L

L

E

A

I

Y

M

A

K

I

V

A

Q

M

E

E

F

I

S

S

R

R

G

G

P

C

G

A

G

S

M

T

R

G

M

V

H

I

L

M

H

S

H

V

A

N

G

S

L

L

N

Y

W

L

R

G

I

P

M

I

D

L

D

K

F

F

A

G

Q

S

P

K

E

E

L

Q

K

K

E

Q

-

A

W

W

A

V

M

T

P

P

R

-

F

F

Q

T

N

S

K

G

T

D

L

K

F

I

V

G

N

C

F
|
F

A

A

L
|
L

T
x
S

E

E

K

P

E

G

A

N

G
|
G

S
|
S

G

D

A

A

D

G

I

A

K

A

T

S

T

T

A

T

V

A

R

R

-

A

D

E

G

G

D

D

E

S

W

W

V

V

I

L

N

N

G

G

E

T

K

K

T

A

L
x
W

I

I

S
x
T

H

N

T

A

D

W

C

E

S

A

D

S

G

A

T

A

Y

V

L

V

I

F

T

A

L

S

T

T

N

D

P

-

E

R

A

A

D

L

K

Q

D

N

K

K

R

G

L

I

T

S

A

A

F

F

F

L

V

V

P

P

T

M

D

P

T

T

P

P

G

G

Y

L

E

T

I

L

V

G

D

K

M

K

P

E

H

D

M

K

M

L

G

G

C

I

R

R

G

G

A

S

G

S

H
x
T

A

A

G

N

L

L

R

I

F

F

T

E

D

D

C

C

R

R

V

I

P

P

D

K

K

D

Y

S

R

I

L

L

G

G

E

E

V

P

G

G

E

M

G

G

L

F

H

K

V

I

A

A

M

M

Y

Q

S

T

L

L

G

D

L

M

S

G

R

R

L

I

H

G

I

I

A

A

D

S

S

Q

N

A

L

L

G

G

M

I

A

A

Q

Q

R

T

M

A

L

L

E

D

M

C

S

A

I

V

A

N

R

Y

A

A

K

E

E

N

R
|
R

I

M

T

A

F
|
F

G

G

K

A

P

P

L
|
L

I

T

K

K

R
x
L

Q

Q

A
x
V

I

I

Q

Q

T

F

M

K

I

L

A

A

E

D

S

M

G

A

K

L

W

A

I

L

Y

E

L

S

L

A

R

R

S

L

A

L

I

T

H

W

D

R

A

A

R

M

R

L

Y

K

D

D

A

N

G

K

E

K

D

P

P

F

M

I

T

K

Q

E

A

A

S

A

L

M

C

A

K

K

L

L

A

A

S

A

I

S

D

E

A

A

V

A

K

T

I

A

V

I

S

S

D

H

N

Q

I

A

L

I

E
x
Q

I
|
I

L

L

G

G

G
|
G

I

M

G

G

Y

Y

F

V

E

T

E

E

C

M

E

-

Y

-

G

-

P

P

A

A

E

E

R

R

L

H

Y

Y

R

R

D

D

C

A

R

R

A
x
I

M

T

W

E

L

I

E
x
Y

E

E

G

G

P
x
T

P

S

S
x
E

V

I

Q

Q

R

R

T
x
L

T

V

A

I

A

A

R

G

G

H

L

L

I

L

T

R

S

S

binding flavin-adenine dinucleotide: F128 (= F126), L130 (= L128), S131 (≠ T129), G136 (= G134), S137 (= S135), W161 (≠ L158), T163 (≠ S160), T214 (≠ H212), R273 (= R271), F276 (= F274), L280 (= L278), L283 (≠ R281), V285 (≠ A283), Q341 (≠ E339), I342 (= I340), G345 (= G343), I363 (≠ A364), Y367 (≠ E368), T370 (≠ P371), E372 (≠ S373), L376 (≠ T377)

5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
31% identity, 96% coverage: 8:384/393 of query aligns to 4:377/378 of 5ol2F

query
sites
5ol2F

N

N

D

S

E

K

F

Y

Q

Q

Q

M

Y

L

L

K

M

E

E

L

I

Y

R

V

E

S

L

F

A

A

Q

E

G

N

P

E

F

V

D

K

E

P

L

L

Q

A

T

T

E
|
E

I

L

E

D

V

E

T
x
E

N

E

K
x
R

F

F

P

P

D

Y

E

E

F

T

Y

V

E

E

L

K

A

M

K

A

E

K

H

A

N

G

L

M

Y

M

R

G

F

I

Y

P

M

Y

P

P

E

K

Q

E

Y

Y

G

G

W

E

G

G

L

G

S

D

T

T

L

V

E

G

I

Y

M

I

K

M

V

A

Q

V

E

E

F

L

S

S

R

R

-

V

-

C

G

G

P

T

G

T

G

G

M

V

R

I

M

L

H

S

L

A

H

H

H

T

A

S

A

L

G

G

L

-

N

S

W

W

R

P

I

I

M

Y

D

Q

D

Y

F

G

A

N

Q

E

P

E

E

Q

L

K

K

Q

E

K

W

F

A

L

M

R

P

P

R

L

F

A

Q

S

N

G

K

E

T

K

L

L

F

G

V

A

N

-

F
|
F

A

G

L
|
L

T
|
T

E

E

K
x
P

E

N

A

A

G

G

S
x
T

G

D

A

A

D

S

-

G

I

Q

K

Q

T

T

A

A

V

V

R

L

D

D

G

G

D

D

E

E

W

Y

V

I

I

L

N

N

G

G

E

S

K

K

T

I

L
x
F

I
|
I

S
x
T

H

N

T

A

D

I

C

A

S

G

D

D

G

-

T

I

Y

Y

L

V

I

V

T

M

L

A

T

M

N

T

P

D

E

K

A

S

D

K

K

G

D

N

K

K

R

G

L

I

T

S

A

A

F

F

F

I

V

V

P

E

T

K

D

G

T

T

P

P

G

G

Y

F

E

S

I

F

V

G

D

V

M

K

P
x
E

H

K

M

K

M

M

G

G

C

I

R

R

G

G

A

S

G

A

H

T

A

S

G

E

L

L

R

I

F

F

T

E

D

D

C

C

R

R

V

I

P

P

D

K

K

E

Y

N

R

L

L

L

G

G

E

K

V

E

G

G

E

Q

G

G

L

F

H

K

V

I

A

A

M

M

Y

S

S

T

L
|
L

G

D

L

G

S
x
G

R
|
R

L

I

H

G

I

I

A

A

D

A

S

Q

N

A

L

L

G

G

M

L

A

A

Q

Q

R

G

M

A

L

L

E

D

M

E

S

T

I

V

A

K

R

Y

A

V

K

K

E

E

R
|
R

I

V

T

Q

F
|
F

G

G

K

R

P

P

L
|
L

I

S

K

K

R
x
F

Q

Q

A

N

I

T

Q

Q

T

F

M

Q

I

L

A

A

E

D

S

M

G

E

K

V

W

K

I

V

Y

Q

L

A

R

R

S

H

A

L

I

V

H

Y

D

Q

A

A

R

I

R

N

Y

K

D

D

A

L

G

G

E

K

D

P

P

Y

M

G

T

V

Q

E

A

A

S

A

L

M

C

A

K

K

L

L

A

F

S

A

I

A

D

E

A

T

V

A

K

M

I

E

V

V

S

T

D

T

N

K

I

A

L

V

E
x
Q

I
x
L

L

H

G
|
G

I

Y

G

G

Y

Y

F

T

E

R

E

D

C

Y

E

-

Y

-

G

-

P

P

A

V

E

E

R

R

L

M

Y

M

R

R

D

D

C

A

R

K

A

I

M

T

W

E

L

I

E
x
Y

E
|
E

G
|
G

P
x
T

P

S

S
x
E

V

V

Q

Q

R

R

T

M

T

V

A

I

A

S

R
x
G

G

K

L

L

I

L

active site: L124 (= L128), T125 (= T129), G241 (≠ S245), G374 (≠ R381)
binding calcium ion: E29 (= E33), E33 (≠ T37), R35 (≠ K39)
binding coenzyme a persulfide: L238 (= L242), R242 (= R246), E362 (= E369), G363 (= G370)
binding flavin-adenine dinucleotide: F122 (= F126), L124 (= L128), T125 (= T129), P127 (≠ K131), T131 (≠ S135), F155 (≠ L158), I156 (= I159), T157 (≠ S160), E198 (≠ P202), R267 (= R271), F270 (= F274), L274 (= L278), F277 (≠ R281), Q335 (≠ E339), L336 (≠ I340), G338 (= G342), G339 (= G343), Y361 (≠ E368), T364 (≠ P371), E366 (≠ S373)

8i4rA Crystal structure of acyl-coa dehydrogenase complexed with acetyl-coa from thermobifida fusca
30% identity, 95% coverage: 10:384/393 of query aligns to 8:380/381 of 8i4rA

query
sites
8i4rA

E

E

F

H

Q

E

Q

A

Y

L

L

R

M

E

E

A

I

I

R

R

E

S

L

V

A

A

Q

E

G

D

P

K

F

I

D

A

E

P

L

H

Q

A

T

A

E

D

I

V

E

D

V

E

T

Q

N

S

K

R

F

F

P

P

D

Q

E

E

F

A

Y

Y

E

E

L

A

A

L

K

R

E

A

H

S

N

D

L

F

Y

H

R

A

F

P

Y

H

M

V

P

A

E

E

Q

E

Y

Y

G

G

W

V

G

G

L

A

S

D

T

A

L

L

E

A

I

T

M

C

K

I

V

V

Q

I

E

E

F

I

S

A

R

R

G

V

P

C

G

A

G

S

M

S

R

S

M

L

H

-

L

I

H

P

H

A

A

V

A

N

G

K

L

L

N

G

W

S

R

M

I

P

M

L

D

I

D

L

F

S

A

G

Q

S

P

D

E

E

L

V

K

K

E

Q

W

R

A

Y

M

L

P

P

R

E

F

L

Q

A

N

S

K

G

T

E

L

A

F

M

V

F

N

S

F
x
Y

A

G

L
|
L

T
x
S

E

E

K

R

E

E

A

A

G
|
G

S
|
S

G

D

-
x
T

A

A

D

S

I

M

K

R

T

T

T

R

A

A

V

V

R

R

D

D

G

G

D

D

E

D

W

W

V

I

I

L

N

N

G

G

E

Q

K

K

T

S

L
x
W

I
|
I

S
x
T

H

N

T

A

D

G

C

I

S

S

D

K

G

Y

T

Y

Y

T

L

V

I

M

T

A

L

V

T

T

N

D

P

P

E

D

A

G

D

P

K

R

D

G

K
x
R

R

N

L

I

T

S

A

A

F

F

F

V

V

V

P

H

T

I

D

D

T

D

P

P

G

G

Y

F

E

S

I

F

V

G

D

E

M

P

P

E

H

R

M

K

M

L

G

G

C

I

R

K

G

G

A

S

G

P

H

T

A

R

G

E

L

L

R

I

F

F

T

D

D

N

C

V

R

R

V

I

P

P

D

G

K

D

Y

R

R

L

L

V

G

G

E

K

V

V

G

G

E

E

G

G

L

L

H
x
R

V

T

A

A

M
x
L

Y
x
R

S

T

L
|
L

G
x
D

L

H

S

T

R
|
R

L

V

H

T

I

I

A

G

D

A

S

Q

N

A

L

V

G

G

M

I

A

A

Q

Q

R

G

M

A

L

L

E

D

M

Y

S

A

I

L

A

G

R

Y

A

V

K

K

E

E

R

R

I

K

T

Q

F

F

G

G

K

K

P

A

L

I

I

A

K

D

R

F

Q

Q

A

G

I

I

Q

Q

T

F

M

M

I

L

A

A

E

D

S

M

G

A

K

M

W

K

I

L

Y

E

L

A

R

R

S

Q

A

M

I

V

H

Y

D

V

A

A

-

A

-

K

-

S

R

E

R

R

Y

D

D

D

A

A

G

D

E

L

D

S

P

F

M

Y

T

G

Q

A

A

A

S

A

L

K

C

C

K

-

L

F

A

A

S

S

I

D

D

V

A

A

V

M

K

E

I

I

V

T

S

T

D

D

N

A

I

V

L

-

E

Q

I

L

L

L

G

G

I

Y

G

G

Y

Y

F

T

E

R

E

D

C

Y

E

-

Y

-

G

-

P

P

A

V

E

E

R

R

L

M

Y

M

R

R

D

D

C

A

R

K

A
x
I

M

T

W

Q

L

I

E

Y

E
|
E

G
|
G

P
x
T

P

N

S
x
Q

V

I

Q

Q

R

R

T

V

A

M

A

A

R
|
R

G

Q

L

L

I

L

binding acetyl coenzyme *a: S132 (= S135), T134 (vs. gap), R180 (≠ K182), R234 (≠ H236), L237 (≠ M239), R238 (≠ Y240), L240 (= L242), D241 (≠ G243), R244 (= R246), E365 (= E369), G366 (= G370), R377 (= R381)
binding flavin-adenine dinucleotide: Y123 (≠ F126), L125 (= L128), S126 (≠ T129), G131 (= G134), S132 (= S135), W156 (≠ L158), I157 (= I159), T158 (≠ S160), I360 (≠ A364), T367 (≠ P371), Q369 (≠ S373)

8i4pA Crystal structure of acyl-coa dehydrogenase from thermobifida fusca
30% identity, 95% coverage: 10:384/393 of query aligns to 8:380/381 of 8i4pA

query
sites
8i4pA

E

E

F

H

Q

E

Q

A

Y

L

L

R

M

E

E

A

I

I

R

R

E

S

L

V

A

A

Q

E

G

D

P

K

F

I

D

A

E

P

L

H

Q

A

T

A

E

D

I

V

E

D

V

E

T

Q

N

S

K

R

F

F

P

P

D

Q

E

E

F

A

Y

Y

E

E

L

A

A

L

K

R

E

A

H

S

N

D

L

F

Y

H

R

A

F

P

Y

H

M

V

P

A

E

E

Q

E

Y

Y

G

G

W

V

G

G

L

A

S

D

T

A

L

L

E

A

I

T

M

C

K

I

V

V

Q

I

E

E

F

I

S

A

R

R

G

V

P

C

G

A

G

S

M

S

R

S

M

L

H

-

L

I

H

P

H

A

A

V

A

N

G

K

L

L

N

G

W

S

R

M

I

P

M

L

D

I

D

L

F

S

A

G

Q

S

P

D

E

E

L

V

K

K

E

Q

W

R

A

Y

M

L

P

P

R

E

F

L

Q

A

N

S

K

G

T

E

L

A

F

M

V

F

N

S

F
x
Y

A

G

L
|
L

T
x
S

E

E

K

R

E

E

A

A

G
|
G

S
|
S

G

D

-

T

A

A

D

S

I

M

K

R

T

T

T

R

A

A

V

V

R

R

D

D

G

G

D

D

E

D

W

W

V

I

I

L

N

N

G

G

E

Q

K

K

T

S

L
x
W

I
|
I

S
x
T

H

N

T

A

D

G

C

I

S

S

D

K

G

Y

T

Y

Y

T

L

V

I

M

T

A

L

V

T

T

N

D

P

P

E

D

A

G

D

P

K

R

D

G

K

R

R

N

L

I

T

S

A

A

F

F

F

V

V

V

P

H

T

I

D

D

T

D

P

P

G

G

Y

F

E

S

I

F

V

G

D

E

M

P

P

E

H

R

M

K

M

L

G

G

C

I

R

K

G

G

A

S

G

P

H

T

A

R

G

E

L

L

R

I

F

F

T

D

D

N

C

V

R

R

V

I

P

P

D

G

K

D

Y

R

R

L

L

V

G

G

E

K

V

V

G

G

E

E

G

G

L

L

H

R

V

T

A

A

M

L

Y

R

S

T

L

L

G

D

L

H

S

T

R

R

L

V

H

T

I

I

A

G

D

A

S

Q

N

A

L

V

G

G

M

I

A

A

Q

Q

R

G

M

A

L

L

E

D

M

Y

S

A

I

L

A

G

R

Y

A

V

K

K

E

E

R

R

I

K

T

Q

F

F

G

G

K

K

P

A

L

I

I

A

K

D

R

F

Q

Q

A

G

I

I

Q

Q

T

F

M

M

I

L

A

A

E

D

S

M

G

A

K

M

W

K

I

L

Y

E

L

A

R

R

S

Q

A

M

I

V

H

Y

D

V

A

A

-

A

-

K

-

S

R

E

R

R

Y

D

D

D

A

A

G

D

E

L

D

S

P

F

M

Y

T

G

Q

A

A

A

S

A

L

K

C

C

K

-

L

F

A

A

S

S

I

D

D

V

A

A

V

M

K

E

I

I

V

T

S

T

D

D

N

A

I

V

L

-

E

Q

I

L

L

L

G

G

I

Y

G

G

Y

Y

F

T

E

R

E

D

C

Y

E

-

Y

-

G

-

P

P

A

V

E

E

R

R

L

M

Y

M

R

R

D

D

C

A

R

K

A
x
I

M

T

W

Q

L

I

E
x
Y

E

E

G

G

P
x
T

P

N

S
x
Q

V

I

Q

Q

R

R

T

V

A

M

A

A

R

R

G

Q

L

L

I

L

binding flavin-adenine dinucleotide: Y123 (≠ F126), L125 (= L128), S126 (≠ T129), G131 (= G134), S132 (= S135), W156 (≠ L158), I157 (= I159), T158 (≠ S160), I360 (≠ A364), Y364 (≠ E368), T367 (≠ P371), Q369 (≠ S373)

1jqiA Crystal structure of rat short chain acyl-coa dehydrogenase complexed with acetoacetyl-coa (see paper)
32% identity, 96% coverage: 10:386/393 of query aligns to 9:382/384 of 1jqiA

query
sites
1jqiA

E

E

E

T

F

H

Q

Q

Q

M

Y

L

L

R

M

Q

E

T

I

C

R

R

E

D

L

F

A

A

Q

E

G

K

P

E

F

L

D

V

E

P

L

I

Q

A

T

A

E

Q

I

L

E

D

V

K

T

E

N

H

K

L

F

F

P

P

D

T

E

S

F

Q

Y

V

E

K

L

K

A

M

K

G

E

E

H

L

N

G

L

L

Y

L

R

A

F

M

Y

D

M

V

P

P

E

E

Q

E

Y

L

G

S

G

G

W

A

G

G

L

L

S

D

T

Y

L

L

E

A

I

Y

M

S

K

I

V

A

Q

L

E

E

F

I

S

S

R

R

G

G

P

C

G

A

-

S

-

T

G

G

M

V

R

I

M

M

H

S

L

V

H

N

A

S

A
x
L

G

Y

L

L

N

G

W

-

R

P

I

I

M

L

D

K

D

F

F

G

A

S

Q

S

P

Q

E

Q

L

K

K

Q

E

Q

W

W

A

I

M

T

P

P

R

-

F

F

Q

T

N

N

K

G

T

D

L

K

F

I

V

G

N

C

F
|
F

A

A

L
|
L

T
x
S

E

E

K

P

E

G

A

N

G
|
G

S
|
S

G

D

A

A

D

G

I

A

K

A

T

S

T

T

A

T

V

A

R

R

D

E

-

E

G

G

D

D

E

S

W

W

V

V

I

L

N

N

G

G

E

T

K

K

T

A

L
x
W

I

I

S
x
T

H

N

T

S

D

W

C

E

S

A

D

S

G

A

T

T

Y

V

L

V

I

F

T

A

L

S

T

T

N

D

P

-

E

R

A

S

D

R

K

Q

D

N

K

K

R

G

L

I

T

S

A

A

F

F

F

L

V

V

P

P

T

M

D

P

T

T

P

P

G

G

Y

L

E

T

I

L

V

G

D

K

M

K

P

E

H

D

M

K

M

L

G

G

C

I

R

R

G

A

A

S

G

S

H

T

A

A

G

N

L

L

R

I

F

F

T

E

D

D

C

C

R

R

V

I

P

P

D

K

K

E

Y

N

R

L

L

L

G

G

E

E

V

P

G

G

E

M

G

G

L
x
F

H

K

V

I

A

A

M
|
M

Y
x
Q

S

T

L
|
L

G
x
D

L

M

S

G

R
|
R

L

I

H

G

I

I

A

A

D

S

S

Q

N

A

L

L

G

G

M

I

A

A

Q

Q

R

A

M

S

L

L

E

D

M

C

S

A

I

V

A

K

R

Y

A

A

K

E

E

N

R
|
R

I

H

T

A

F
|
F

G

G

K

A

P

P

L

L

I

T

K

K

R
x
L

Q

Q

A

N

I

I

Q

Q

T

F

M

K

I

L

A

A

E

D

S

M

G

A

K

L

W

A

I

L

Y

E

L

S

L

A

R

R

S

L

A

L

I

T

H

W

D

R

A

A

R

M

R

L

Y

K

D

D

A

N

G

K

E

K

D

P

P

F

M

T

T

K

Q

E

A

S

S

A

L

M

C

A

K

K

L

L

A

A

S

A

I

S

D

E

A

A

V

A

K

T

I

A

V

I

S

S

D

H

N

Q

I

A

L

I

E
x
Q

I
|
I

L

L

G

G

G
|
G

I

M

G

G

Y

Y

F

V

E

T

E

E

C

M

E

-

Y

-

G

-

P

P

A

A

E

E

R

R

L

Y

Y

Y

R

R

D

D

C

A

R

R

A
x
I

M

T

W

E

L

I

E
x
Y

E
|
E

G
|
G

P
x
T

P

S

S
x
E

V
x
I

Q

Q

R

R

T

L

T

V

A

I

A

A

R
x
G

G

H

L

L

I

L

T

R

S

S

active site: G377 (≠ R381)
binding acetoacetyl-coenzyme a: L95 (≠ A94), F125 (= F126), S134 (= S135), F234 (≠ L235), M238 (= M239), Q239 (≠ Y240), L241 (= L242), D242 (≠ G243), R245 (= R246), Y364 (≠ E368), E365 (= E369), G366 (= G370)
binding flavin-adenine dinucleotide: F125 (= F126), L127 (= L128), S128 (≠ T129), G133 (= G134), S134 (= S135), W158 (≠ L158), T160 (≠ S160), R270 (= R271), F273 (= F274), L280 (≠ R281), Q338 (≠ E339), I339 (= I340), G342 (= G343), I360 (≠ A364), T367 (≠ P371), E369 (≠ S373), I370 (≠ V374)

7w0jE Acyl-coa dehydrogenase, tfu_1647
30% identity, 95% coverage: 10:384/393 of query aligns to 9:381/382 of 7w0jE

query
sites
7w0jE

E

E

F

H

Q

E

Q

A

Y

L

L

R

M

E

E

A

I

I

R

R

E

S

L

V

A

A

Q

E

G

D

P

K

F

I

D

A

E

P

L

H

Q

A

T

A

E

D

I

V

E

D

V

E

T

Q

N

S

K

R

F

F

P

P

D

Q

E

E

F

A

Y

Y

E

E

L

A

A

L

K

R

E

A

H

S

N

D

L

F

Y

H

R

A

F

P

Y

H

M

V

P

A

E

E

Q

E

Y

Y

G

G

W

V

G

G

L

A

S

D

T

A

L

L

E

A

I

T

M

C

K

I

V

V

Q

I

E

E

F

I

S

A

R

R

G

V

P

C

G

A

G

S

M

S

R

S

M

L

H

-

L

I

H

P

H

A

A

V

A

N

G

K

L

L

N

G

W

S

R

M

I

P

M

L

D

I

D

L

F

S

A

G

Q

S

P

D

E

E

L

V

K

K

E

Q

W

R

A

Y

M

L

P

P

R

E

F

L

Q

A

N

S

K

G

T

E

L

A

F

M

V

F

N

S

F

Y

A

G

L

L

T
x
S

E

E

K

R

E

E

A

A

G

G

S

S

G

D

-

T

A

A

D

S

I

M

K

R

T

T

T

R

A

A

V

V

R

R

D

D

G

G

D

D

E

D

W

W

V

I

I

L

N

N

G

G

E

Q

K

K

T

S

L
x
W

I

I

S

T

H

N

T

A

D

G

C

I

S

S

D

K

G

Y

T

Y

Y

T

L

V

I

M

T

A

L

V

T

T

N

D

P

P

E

D

A

G

D

P

K

R

D

G

K

R

R

N

L

I

T

S

A

A

F

F

F

V

V

V

P

H

T

I

D

D

T

D

P

P

G

G

Y

F

E

S

I

F

V

G

D

E

M

P

P

E

H

R

M

K

M

L

G

G

C

I

R

K

G

G

A

S

G

P

H

T

A

R

G

E

L

L

R

I

F

F

T

D

D

N

C

V

R

R

V

I

P

P

D

G

K

D

Y

R

R

L

L

V

G

G

E

K

V

V

G

G

E

E

G

G

L

L

H

R

V

T

A

A

M

L

Y

R

S

T

L

L

G

D

L

H

S

T

R

R

L

V

H

T

I

I

A

G

D

A

S

Q

N

A

L

V

G

G

M

I

A

A

Q

Q

R

G

M

A

L

L

E

D

M

Y

S

A

I

L

A

G

R

Y

A

V

K

K

E

E

R
|
R

I

K

T
x
Q

F
|
F

G

G

K

K

P

A

L
x
I

I

A

K

D

R
x
F

Q

Q

A

G

I
|
I

Q

Q

T

F

M

M

I

L

A

A

E

D

S

M

G

A

K

M

W

K

I

L

Y

E

L

A

R

R

S

Q

A

M

I

V

H

Y

D

V

A

A

-

A

-

K

-

S

R

E

R

R

Y

D

D

D

A

A

G

D

E

L

D

S

P

F

M

Y

T

G

Q

A

A

A

S

A

L

K

C

C

K

-

L

F

A

A

S

S

I

D

D

V

A

A

V

M

K

E

I

I

V

T

S

T

D

D

N

A

I

V

L

-

E
x
Q

I
x
L

L

L

G

G

G
|
G

I

Y

G

G

Y

Y

F

T

E

R

E

D

C

Y

E

-

Y

-

G

-

P

P

A

V

E

E

R

R

L

M

Y

M

R

R

D

D

C

A

R

K

A

I

M

T

W

Q

L

I

E
x
Y

E
|
E

G

G

P
x
T

P

N

S
x
Q

V
x
I

Q

Q

R

R

T

V

A

M

A

A

R

R

G

Q

L

L

I

L

binding [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3S,4S)-5-azanyl-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: S127 (≠ T129), W157 (≠ L158), R270 (= R271), Q272 (≠ T273), F273 (= F274), I277 (≠ L278), F280 (≠ R281), I283 (= I284), Q339 (≠ E339), L340 (≠ I340), G343 (= G343), Y365 (≠ E368), E366 (= E369), T368 (≠ P371), Q370 (≠ S373), I371 (≠ V374)

P15651 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Rattus norvegicus (Rat) (see 2 papers)
32% identity, 96% coverage: 10:386/393 of query aligns to 36:409/412 of P15651

query
sites
P15651

E

E

E

T

F

H

Q

Q

Q

M

Y

L

L

R

M

Q

E

T

I

C

R

R

E

D

L

F

A

A

Q

E

G

K

P

E

F

L

D

V

E

P

L

I

Q

A

T

A

E

Q

I

L

E

D

V

K

T

E

N

H

K

L

F

F

P

P

D

T

E

S

F

Q

Y

V

E

K

L

K

A

M

K

G

E

E

H

L

N

G

L

L

Y

L

R

A

F

M

Y

D

M

V

P

P

E

E

Q

E

Y

L

G

S

G

G

W

A

G

G

L

L

S

D

T

Y

L

L

E

A

I

Y

M

S

K

I

V

A

Q

L

E

E

F

I

S

S

R

R

G

G

P

C

G

A

-

S

-

T

G

G

M

V

R

I

M

M

H

S

L

V

H

N

A

S

A

L

G

Y

L

L

N

G

W

-

R

P

I

I

M

L

D

K

D

F

F

G

A

S

Q

S

P

Q

E

Q

L

K

K

Q

E

Q

W

W

A

I

M

T

P

P

R

-

F

F

Q

T

N

N

K

G

T

D

L

K

F

I

V

G

N

C

F
|
F

A
|
A

L
|
L

T
x
S

E
|
E

K
x
P

E
x
G

A
x
N

G
|
G

S
|
S

G

D

A

A

D

G

I

A

K

A

T

S

T

T

A

T

V

A

R

R

D

E

-

E

G

G

D

D

E

S

W

W

V

V

I

L

N

N

G

G

E

T

K

K

T

A

L
x
W

I
|
I

S
x
T

H

N

T

S

D

W

C

E

S

A

D

S

G

A

T

T

Y

V

L

V

I

F

T

A

L

S

T

T

N

D

P

-

E

R

A

S

D

R

K

Q

D

N

K

K

R

G

L

I

T

S

A

A

F

F

F

L

V

V

P

P

T

M

D

P

T

T

P

P

G

G

Y

L

E

T

I

L

V

G

D

K

M

K

P

E

H

D

M

K

M

L

G

G

C

I

R

R

G

A

A

S

G

S

H

T

A

A

G

N

L

L

R

I

F

F

T

E

D

D

C

C

R

R

V

I

P

P

D

K

K

E

Y

N

R

L

L

L

G

G

E

E

V

P

G

G

E

M

G

G

L

F

H

K

V

I

A

A

M

M

Y

Q

S

T

L

L

G
x
D

L
x
M

S
x
G

R
|
R

L

I

H

G

I

I

A

A

D

S

S

Q

N

A

L

L

G

G

M

I

A

A

Q

Q

R

A

M

S

L

L

E

D

M

C

S

A

I

V

A

K

R

Y

A

A

K

E

E

N

R
|
R

I

H

T

A

F

F

G

G

K

A

P

P

L

L

I

T

K

K

R

L

Q

Q

A

N

I

I

Q

Q

T

F

M

K

I

L

A

A

E

D

S

M

G

A

K

L

W

A

I

L

Y

E

L

S

L

A

R

R

S

L

A

L

I

T

H

W

D

R

A

A

R

M

R

L

Y

K

D

D

A

N

G

K

E

K

D

P

P

F

M

T

T

K

Q

E

A

S

S

A

L

M

C

A

K

K

L

L

A

A

S

A

I

S

D

E

A

A

V

A

K

T

I

A

V

I

S

S

D

H

N

Q

I

A

L

I

E
x
Q

I
|
I

L
|
L

G
|
G

I

M

G

G

Y

Y

F

V

E

T

E

E

C

M

E

-

Y

-

G

-

P

P

A

A

E

E

R

R

L

Y

Y

Y

R

R

D

D

C

A

R

R

A

I

M

T

W

E

L

I

E

Y

E
|
E

G

G

P
x
T

P
x
S

S
x
E

V

I

Q

Q

R

R

T

L

T

V

A

I

A

A

R

G

G

H

L

L

I

L

T

R

S

S

152:161 (vs. 126:135, 60% identical) binding FAD
S161 (= S135) binding substrate
WIT 185:187 (≠ LIS 158:160) binding FAD
DMGR 269:272 (≠ GLSR 243:246) binding substrate
R297 (= R271) binding FAD
QILGG 365:369 (≠ EILGG 339:343) binding FAD
E392 (= E369) active site, Proton acceptor
TSE 394:396 (≠ PPS 371:373) binding FAD

Sites not aligning to the query:

1:24 modified: transit peptide, Mitochondrion

2vigB Crystal structure of human short-chain acyl coa dehydrogenase
32% identity, 96% coverage: 10:386/393 of query aligns to 3:369/371 of 2vigB

query
sites
2vigB

E

E

E

T

F

H

Q

Q

Q

M

Y

L

L

L

M

Q

E

T

I

C

R

R

E

D

L

F

A

A

Q

E

G

K

P

E

F

L

D

F

E

P

L

I

Q

A

T

A

E

Q

I

V

E

D

V

K

T

E

N

H

K

L

F

F

P

P

D

A

E

A

F

Q

Y

V

E

K

L

K

A

M

K

G

E

G

H

L

N

G

L

L

Y

L

R

A

F

M

Y

D

M

V

P

P

E

E

Q

E

Y

L

G

G

W

A

G

G

L

L

S

D

T

Y

L

L

E

A

I

Y

M

A

K

I

V

A

Q

M

E

E

F

I

S

S

R

R

G

G

P

C

G

A

-

S

-

T

G

G

M

V

R

I

M

M

H

S

L

V

H

N

A

S

A

L

G

Y

L

L

N

G

W

-

R

P

I

I

M

L

D

K

D

F

F

G

A

S

Q

K

P

E

E

Q

L

K

K

Q

E

A

W

W

A

V

M

T

P

P

R

-

F

F

Q

T

N

S

K

G

T

D

L

K

F

I

V

G

N

C

F

F

A

A

L
|
L

T
x
S

E

E

K

P

E

G

A

N

G
|
G

S
|
S

G

D

A

A

D

G

I

A

K

A

T

S

T

T

A

T

V

A

R

R

-

A

D

E

G

G

D

D

E

S

W

W

V

V

I

L

N

N

G

G

E

T

K

K

T

A

L
x
W

I

I

S
x
T

H

N

T

A

D

W

C

E

S

A

D

S

G

A

T

A

Y

V

L

V

I

F

T

A

L

S

T

T

N

S

P

-

E

-

A

-

D

-

K

-

D

-

K

-

R

-

L

I

T

S

A

A

F

F

F

L

V

V

P

P

T

M

D

P

T

T

P

P

G

G

Y

L

E

T

I

L

V

G

D

K

M

K

P

E

H

D

M

K

M

L

G

G

C

I

R

R

G

G

A

S

G

S

H

T

A

A

G

N

L

L

R

I

F

F

T

E

D

D

C

C

R

R

V

I

P

P

D

K

K

D

Y

S

R

I

L

L

G

G

E

E

V

P

G

G

E

M

G

G

L
x
F

H

K

V

I

A

A

M
|
M

Y
x
Q

S

T

L
|
L

G
x
D

L

M

S
x
G

R
|
R

L

I

H

G

I

I

A

A

D

S

S

Q

N

A

L

L

G

G

M

I

A

A

Q

Q

R

T

M

A

L

L

E

D

M

C

S

A

I

V

A

N

R

Y

A

A

K

E

E

N

R
|
R

I

M

T

A

F
|
F

G

G

K

A

P

P

L
|
L

I

T

K

K

R
x
L

Q

Q

A

V

I

I

Q

Q

T

F

M

K

I

L

A

A

E

D

S

M

G

A

K

L

W

A

I

L

Y

E

L

S

L

A

R

R

S

L

A

L

I

T

H

W

D

R

A

A

R

M

R

L

Y

K

D

D

A

N

G

K

E

K

D

P

P

F

M

I

T

K

Q

E

A

A

S

A

L

M

C

A

K

K

L

L

A

A

S

A

I

S

D

E

A

A

V

A

K

T

I

A

V

I

S

S

D

H

N

Q

I

A

L

I

E
x
Q

I
|
I

L

L

G

G

G
|
G

I

M

G

G

Y

Y

F

V

E

T

E

E

C

M

E

-

Y

-

G

-

P

P

A

A

E

E

R

R

L

H

Y

Y

R

R

D

D

C

A

R

R

A
x
I

M

T

W

E

L

I

E
x
Y

E
|
E

G
|
G

P
x
T

P

S

S
x
E

V
x
I

Q

Q

R

R

T

L

T

V

A

I

A

A

R
x
G

G

H

L

L

I

L

T

R

S

S

active site: L121 (= L128), S122 (≠ T129), G231 (≠ S245), E352 (= E369), G364 (≠ R381)
binding coenzyme a persulfide: S128 (= S135), F221 (≠ L235), M225 (= M239), Q226 (≠ Y240), L228 (= L242), D229 (≠ G243), R232 (= R246), E352 (= E369), G353 (= G370), I357 (≠ V374)
binding flavin-adenine dinucleotide: L121 (= L128), S122 (≠ T129), G127 (= G134), S128 (= S135), W152 (≠ L158), T154 (≠ S160), R257 (= R271), F260 (= F274), L264 (= L278), L267 (≠ R281), Q325 (≠ E339), I326 (= I340), G329 (= G343), I347 (≠ A364), Y351 (≠ E368), T354 (≠ P371), E356 (≠ S373)

3pfdC Crystal structure of an acyl-coa dehydrogenase from mycobacterium thermoresistibile bound to reduced flavin adenine dinucleotide solved by combined iodide ion sad mr (see paper)
29% identity, 93% coverage: 19:384/393 of query aligns to 8:368/369 of 3pfdC

query
sites
3pfdC

I

I

R

R

E

A

L

L

A

A

Q

E

G

K

P

E

F

I

D

A

E

P

L

Y

Q

A

T

A

E

E

I

V

E

D

V

E

T

K

N

A

K

R

F

F

P

P

D

E

E

E

F

A

Y

L

E

A

L

A

A

L

K

N

E

S

H

S

N

G

L

F

Y

S

R

A

F

I

Y

H

M

V

P

P

E

E

Q

E

Y

Y

G

G

W

Q

G

G

L

A

S

D

T

S

L

V

E

A

I

T

M

C

K

I

V

V

Q

I

E

E

F

V

S

A

R

R

G

V

P

D

G

C

G

S

M

A

R

S

M

L

-

I

-

P

-

A

-

V

H

N

L

K

H

L

H

G

A

T

A

M

G

G

L

L

N

I

W

L

R

R

I

-

M

-

D

-

F

-

A

G

Q

S

P

E

E

E

L

L

K

K

E

K

W

Q

A

V

M

L

P

P

R

A

F

V

Q

A

N

S

K

G

T

E

L

A

F

M

V

A

N

S

F
x
Y

A

A

L
|
L

T
x
S

E

E

K

R

E

E

A

A

G
|
G

S
|
S

-

D

G

A

A

A

D

S

I

M

K

R

T

T

T

R

A

A

V

V

R

A

D

D

G

G

D

D

E

D

W

W

V

I

I

L

N

N

G

G

E

S

K

K

T

C

L
x
W

I
|
I

S
x
T

H

N

T

G

D

G

C

K

S

S

D

T

G

W

T

Y

Y

T

L

V

I

M

T

A

L

V

T

T

N

D

P

P

E

D

A

K

D

G

K

A

D

N

K

G

R

-

L

I

T

S

A

A

F

F

F

M

V

V

P

H

T

K

D

D

T

D

P

E

G

G

Y

F

E

T

I

V

V

G

D

P

M

K

P

E

H

R

M

K

M

L

G

G

C

I

R

K

G

G

A

S

G

P

H

T

A

T

G

E

L

L

R

Y

F

F

T

E

D

N

C

C

R

R

V

I

P

P

D

G

K

D

Y

R

R

I

L

I

G

G

E

E

V

P

G

G

E

T

G

G

L

F

H

K

V

T

A

A

M

L

Y

A

S

T

L

L

G

D

L

H

S
x
T

R

R

L

P

H

T

I

I

A

G

D

A

S

Q

N

A

L

V

G

G

M

I

A

A

Q

Q

R

G

M

A

L

L

E

D

M

A

S

A

I

I

A

A

R

Y

A

T

K

K

E

E

R
|
R

I

K

T

Q

F
|
F

G

G

K

R

P

P

L
x
V

I

S

K

D

R
x
N

Q

Q

A

G

I

V

Q

Q

T

F

M

M

I

L

A

A

E

D

S

M

G

A

K

M

W

K

I

I

Y

E

L

A

R

R

S

L

A

M

I

V

H

Y

D

S

A

A

R

A

R

R

Y

A

D

E

A

R

G

G

E

D

D

L

P

G

M

F

T

I

Q

S

A

A

S

A

L

S

C

K

K

C

L

F

A

A

S

S

I

D

D

V

A

A

V

M

K

E

I

V

V

T

S

T

D

D

N

A

I

V

L

-

E
x
Q

I
x
L

L

F

G

G

G
|
G

I

Y

G

G

Y

Y

F

T

E

Q

E

D

C

F

E

-

Y

-

G

-

P

P

A

V

E

E

R

R

L

M

Y

M

R

R

D

D

C

A

R

K

A
x
I

M

T

W

Q

L

I

E
x
Y

E
|
E

G

G

P
x
T

P

N

S
x
Q

V

I

Q

Q

R

R

T

V

A

M

A

S

R
|
R

G

A

L

L

I

L

active site: L116 (= L128), S117 (≠ T129), T233 (≠ S245), E353 (= E369), R365 (= R381)
binding dihydroflavine-adenine dinucleotide: Y114 (≠ F126), L116 (= L128), S117 (≠ T129), G122 (= G134), S123 (= S135), W147 (≠ L158), I148 (= I159), T149 (≠ S160), R259 (= R271), F262 (= F274), V266 (≠ L278), N269 (≠ R281), Q326 (≠ E339), L327 (≠ I340), G330 (= G343), I348 (≠ A364), Y352 (≠ E368), T355 (≠ P371), Q357 (≠ S373)

2a1tC Structure of the human mcad:etf e165betaa complex (see paper)
31% identity, 95% coverage: 10:381/393 of query aligns to 10:380/388 of 2a1tC

query
sites
2a1tC

E

E

E

Q

F

Q

Q

K

Q

E

Y

F

L

Q

M

A

E

T

I

A

R

R

E

K

L

F

A

A

Q

R

G

E

P

E

F

I

D

I

E

P

L

V

Q

A

T

A

E

E

I

Y

E

D

V

K

T

T

N

G

K

E

F

Y

P

P

D

V

E

P

F

L

Y

I

E

R

L

R

A

A

K

W

E

E

H

L

N

G

L

L

Y

M

R

N

F

T

Y

H

M

I

P

P

E

E

Q

N

Y

C

G

G

W

L

G

G

L

L

S

G

T

T

L

F

E

D

I

A

M

C

K

L

V

I

Q

S

E

E

F

L

S

A

R

Y

G

G

P

C

G

T

G

G

M

V

R

Q

M

T

H

A

L

I

H

E

H

G

A

N

A

S

G

L

L

G

N

Q

W

M

R

P

I

I

M

I

D

I

D

A

F

G

A

N

Q

D

P

Q

E

Q

L

K

K

K

E

K

W

Y

A

-

M

L

P

G

R

R

F

M

Q

T

N

E

K

E

T

P

L

L

F

M

V

C

N

A

F
x
Y

A

C

L
x
V

T
|
T

E

E

K

P

E

G

A

A

G
|
G

S
|
S

G

D

-

V

A

A

D

G

I

I

K

K

T

T

T

K

A

A

V

E

R

K

D

K

G

G

D

D

E

E

W

Y

V

I

I

I

N

N

G

G

E
x
Q

K

K

T

M

L
x
W

I
|
I

S
x
T

H

N

T

G

D

G

C

K

S

A

D

N

G

W

T

Y

Y

F

L

L

I

L

T

A

L

R

T

S

N

D

P

P

E

D

-

P

-

K

A

A

D

P

K

A

D

N

K

K

R

A

L

F

T

T

A

G

F

F

F

I

V

V

P

E

T

A

D

D

T

T

P

P

G

G

Y

I

E

Q

I

I

V

G

D

R

M

K

P

E

H

L

M

N

M

M

G

G

C

Q

R

R

G

C

A

S

G

D

H

T

A

R

G

G

L

I

R

V

F

F

T

E

D

D

C

V

R

K

V

V

P

P

D

K

K

E

Y

N

R

V

L

L

G

I

E

G

V

D

G

G

E

A

G

G

L

F

H

K

V

V

A

A

M

M

Y

G

S

A

L

F

G

D

L

K

S
x
T

R

R

L

P

H

V

I

V

A

A

D

A

S

G

N

A

L

V

G

G

M

L

A

A

Q

Q

R

R

M

A

L

L

E

D

M

E

S

A

I

T

A

K

R

Y

A

A

K

L

E

E

R
|
R

I

K

T
|
T

F
|
F

G

G

K

K

P

L

L
|
L

I

V

K

E

R
x
H

Q

Q

A

A

I
|
I

Q

S

T

F

M

M

I

L

A

A

E

E

S

M

G

A

K

M

W

K

I

V

Y

E

L

L

L

A

R

R

S

M

A

S

I

Y

H

Q

D

R

A

A

R

W

R

E

Y

V

D

D

A

S

G

G

E

R

D

R

P

N

M

T

T

Y

Q

Y

A

A

S

S

L

I

C

A

K

K

L

A

A

F

S

A

I

G

D

D

A

I

V

A

K

N

I

Q

V

L

S

A

D

T

N

D

I

A

L

V

E
x
Q

I
|
I

L

L

G

G

G
|
G

I

N

G

G

Y

F

F

-

E

-

E

N

C

T

E

E

Y

Y

G

-

P

P

A

V

E

E

R

K

L

L

Y

M

R

R

D

D

C

A

R

K

A
x
I

M

Y

W

Q

L

I

E

Y

E
|
E

G

G

P
x
T

P

S

S
x
Q

V

I

Q

Q

R

R

T

L

T

I

A

V

A

A

R
|
R

active site: V127 (≠ L128), T128 (= T129), T247 (≠ S245), E368 (= E369), R380 (= R381)
binding flavin-adenine dinucleotide: Y125 (≠ F126), V127 (≠ L128), T128 (= T129), G133 (= G134), S134 (= S135), Q155 (≠ E155), W158 (≠ L158), W158 (≠ L158), I159 (= I159), T160 (≠ S160), R273 (= R271), T275 (= T273), F276 (= F274), L280 (= L278), H283 (≠ R281), I286 (= I284), Q341 (≠ E339), I342 (= I340), G345 (= G343), I363 (≠ A364), T370 (≠ P371), Q372 (≠ S373)

P11310 Medium-chain specific acyl-CoA dehydrogenase, mitochondrial; MCAD; Medium chain acyl-CoA dehydrogenase; MCADH; EC 1.3.8.7 from Homo sapiens (Human) (see 16 papers)
31% identity, 95% coverage: 10:381/393 of query aligns to 43:413/421 of P11310

query
sites
P11310

E

E

E

Q

F

Q

Q

K

Q

E

Y

F

L

Q

M

A

E

T

I

A

R

R

E

K

L

F

A

A

Q

R

G

E

P

E

F

I

D

I

E

P

L

V

Q

A

T

A

E

E

I
x
Y

E

D

V

K

T

T

N

G

K

E

F

Y

P

P

D

V

E

P

F

L

Y

I

E

R

L

R

A

A

K

W

E

E

H

L

N

G

L
|
L

Y

M

R

N

F

T

Y

H

M

I

P

P

E

E

Q

N

Y

C

G

G

W
x
L

G

G

L
|
L

S

G

T

T

L

F

E

D

I

A

M

C

K

L

V
x
I

Q

S

E

E

F

L

S

A

R

Y

G

G

P

C

G

T

G

G

M

V

R

Q

M

T

H

A

L

I

H

E

H

G

A

N

A

S

G

L

L

G

N

Q

W

M

R
x
P

I

I

M

I

D

I

D

A

F

G

A

N

Q

D

P

Q

E

Q

L

K

K

K

E

K

W

Y

A

-

M

L

P

G

R

R

F

M

Q

T

N

E

K

E

T

P

L

L

F

M

V

C

N

A

F
x
Y

A
x
C

L
x
V

T
|
T

E
|
E

K
x
P

E
x
G

A
|
A

G
|
G

S
|
S

G

D

-

V

A

A

D

G

I

I

K

K

T

T

T

K

A

A

V

E

R

K

D

K

G

G

D

D

E

E

W

Y

V

I

I

I

N

N

G

G

E

Q

K

K

T

M

L
x
W

I
|
I

S
x
T

H

N

T

G

D

G

C

K

S

A

D

N

G

W

T

Y

Y

F

L

L

I

L

T

A

L

R

T

S

N

D

P

P

E

D

-

P

-

K

A

A

D

P

K

A

D

N

K

K

R

A

L

F

T

T

A

G

F

F

F

I

V

V

P

E

T

A

D

D

T

T

P

P

G

G

Y

I

E

Q

I

I

V

G

D

R

M

K

P
x
E

H

L

M

N

M

M

G

G

C

Q

R

R

G

C

A

S

G

D

H

T

A

R

G

G

L

I

R

V

F

F

T

E

D

D

C

V

R

K

V

V

P

P

D

K

K

E

Y

N

R

V

L

L

G

I

E

G

V

D

G

G

E

A

G

G

L

F

H

K

V

V

A

A

M

M

Y

G

S

A

L

F

G
x
D

L

K

S
x
T

R
|
R

L

P

H

V

I

V

A

A

D

A

S

G

N

A

L

V

G

G

M

L

A

A

Q

Q

R

R

M

A

L

L

E

D

M

E

S

A

I

T

A

K

R

Y

A

A

K

L

E

E

R
|
R

I
x
K

T
|
T

F

F

G

G

K

K

P

L

L

L

I

V

K

E

R
x
H

Q
|
Q

A

A

I

I

Q

S

T

F

M

M

I

L

A

A

E

E

S

M

G

A

K

M

W
x
K

I

V

Y

E

L

L

L

A

R

R

S

M

A

S

I

Y

H

Q

D

R

A

A

R

W

R

E

Y

V

D

D

A

S

G

G

E

R

D

R

P

N

M

T

T

Y

Q

Y

A

A

S

S

L

I

C

A

K

K

L

A

A

F

S

A

I

G

D

D

A

I

V

A

K

N

I

Q

V

L

S

A

D

T

N

D

I

A

L

V

E
x
Q

I
|
I

L
|
L

G
|
G

I

N

G

G

Y

F

F

-

E

-

E

N

C

T

E
|
E

Y

Y

G

-

P

P

A

V

E

E

R

K

L

L

Y

M

R

R

D

D

C

A

R

K

A

I

M

Y

W

Q

L

I

E

Y

E
|
E

G
|
G

P
x
T

P
x
S

S
x
Q

V

I

Q

Q

R

R

T

L

T

I

A

V

A

A

R

R

Y67 (≠ I34) to H: in ACADMD; mild; dbSNP:rs121434280
L86 (= L53) mutation to M: Strongly reduced rate of electron transfer to ETF.
L98 (≠ W65) mutation to W: Strongly reduced rate of electron transfer to ETF.
L100 (= L67) mutation to Y: Strongly reduced rate of electron transfer to ETF.
I108 (≠ V75) mutation to M: Strongly reduced rate of electron transfer to ETF.
P132 (≠ R99) to R: in a breast cancer sample; somatic mutation; dbSNP:rs875989854
158:167 (vs. 126:135, 50% identical) binding in other chain
S167 (= S135) binding octanoyl-CoA
W191 (≠ L158) mutation to A: Loss of electron transfer to ETF.; mutation to F: Reduces rate of electron transfer to ETF about six-fold.
WIT 191:193 (≠ LIS 158:160) binding in other chain
T193 (≠ S160) to A: in ACADMD; the thermostability is markedly decreased; dbSNP:rs121434279
E237 (≠ P202) mutation to A: Strongly reduced rate of electron transfer to ETF.
D278 (≠ G243) binding octanoyl-CoA
T280 (≠ S245) mutation to E: Narrower substrate specificity. Changed substrate specificity towards longer acyl chains; when associated with G-401. Loss of acyl-CoA dehydrogenase activity; when associated with T-410.
R281 (= R246) binding octanoyl-CoA; to T: in ACADMD; mild clinical phenotype; dbSNP:rs121434282
RKT 306:308 (≠ RIT 271:273) binding FAD
HQ 316:317 (≠ RQ 281:282) binding in other chain
K329 (≠ W294) to E: in ACADMD; may alter splicing; decreased fatty acid beta-oxidation; dbSNP:rs77931234
QILGG 374:378 (≠ EILGG 339:343) binding FAD
E384 (= E351) mutation to A: Reduces rate of electron transfer to ETF three-fold.; mutation to Q: Reduces rate of electron transfer to ETF two-fold.
E401 (= E369) active site, Proton acceptor; binding octanoyl-CoA; mutation to G: Changed substrate specificity towards longer acyl chains; when associated with E-280.; mutation to Q: Loss of acyl-CoA dehydrogenase activity.; mutation to T: Loss of acyl-CoA dehydrogenase activity; when associated with E-280.
EGTSQ 401:405 (≠ EGPPS 369:373) binding in other chain

4n5fA Crystal structure of a putative acyl-coa dehydrogenase with bound fadh2 from burkholderia cenocepacia j2315
32% identity, 88% coverage: 40:383/393 of query aligns to 38:378/378 of 4n5fA

query
sites
4n5fA

F

L

P

P

D

D

E

A

F

I

Y

V

E

A

L

Q

A

L

K

G

E

E

H

L

N

G

L

L

Y

L

R

G

F

M

Y

I

M

V

P

P

E

Q

Q

E

Y

L

G

G

W

S

G

Y

L

T

S

D

T

Y

L

V

E

A

I

Y

M

A

K

L

V

A

Q

M

E

E

F

V

S

A

R

A

G

G

P

D

G

A

M

C

R

A

-

T

-

M

M

M

H

S

L

V

H

H

H

N

A

S

A

V

G

G

L

C

N

G

W

P

R

I

I

L

M

-

D

-

D

G

F

F

A

G

Q

T

P

P

E

A

L

Q

K

K

E

D

W

R

A

W

M

L

P

A

R

D

F

M

Q

A

N

A

K

G

T

R

L

V

F

I

V

G

N

A

F

F

A

C

L
|
L

T
|
T

E

E

K

P

E

H

A

A

G
|
G

S
|
S

G

E

A

A

D

N

-

N

I

L

K

R

T

T

T

R

A

A

-

E

V

L

R

R

D

D

G

G

D

-

E

Q

W

W

V

V

I

L

N

N

G

G

E

A

K

K

T

Q

L
x
F

I

V

S
x
T

H

N

T

G

D

Q

C

R

S

A

D

G

G

V

T

A

Y

I

L

V

I

F

T

A

L

M

T

T

N

D

P

P

E

E

A

A

D

G

K

K

D

-

K

R

R

G

L

I

T

S

A

A

F

F

F

L

V

V

P

P

T

T

D

D

T

T

P

P

G

G

Y

F

E

I

I

V

V

G

D

K

M

P

P

E

H

K

M

K

M

M

G

G

C

I

R

R

G

A

A

S

G

D

H
x
T

A

C

G

P

L

I

R

T

F

F

T

E

D

N

C

C

R

A

V

I

P

P

D

E

K

D

Y

N

R

L

L

L

G

G

E

N

V

R

G

G

E

E

G

G

L

L

H

K

V

I

A

A

M

L

Y

S

S

N

L

L

G

E

L

G

S
x
G

R

R

L

I

H

G

I

I

A

A

D

A

S

Q

N

A

L

L

G

G

M

I

A

A

Q

R

R

A

M

A

L

F

E

D

M

K

S

A

I

R

A

R

R

Y

A

A

K

G

E

E

R

R

I

V

T

Q

F

F

G

G

K

K

P

P

L

I

I

A

K

E

R

H

Q

Q

A

A

I

I

Q

Q

T

Q

M

K

I

L

A

A

E

D

S

M

G

A

K

V

W

Q

I

I

Y

N

L

A

R

R

S

L

A

L

I

V

H

H

D

H

A

A

R

K

R

L

Y

R

D

T

A

A

G

G

E

L

D

P

P

C

M

L

T

S

Q

E

A

A

S

S

L

Q

C

A

K

K

L

L

A

F

S

A

I

S

D

E

A

M

V

A

K

E

I

R

V

V

S

C

D

S

N

D

I

A

L

I

E

Q

I

I

L

H

G

G

I

Y

G

G

Y

Y

F

L

E

V

E

D

C

Y

E

E

Y

V

G

-

P

-

A

-

E

E

R

R

L

H

Y

Y

R

R

D

D

C

A

R

R

A

I

M

T

W

Q

L

I

E
x
Y

E
|
E

G

G

P
x
T

P

S

S
x
E

V

V

Q

Q

R

R

T
x
M

T

V

A

I

A

A

R
|
R

G

Q

L

L

active site: L126 (= L128), T127 (= T129), G243 (≠ S245), E364 (= E369), R376 (= R381)
binding dihydroflavine-adenine dinucleotide: L126 (= L128), T127 (= T129), G132 (= G134), S133 (= S135), F157 (≠ L158), T159 (≠ S160), T210 (≠ H212), Y363 (≠ E368), T366 (≠ P371), E368 (≠ S373), M372 (≠ T377)

5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
30% identity, 95% coverage: 13:384/393 of query aligns to 5:373/374 of 5lnxD

query
sites
5lnxD

Q

E

Q

Q

Y

V

L

M

M

M

E

R

-

K

-

M

I

V

R

R

E

D

L

F

A

A

Q

R

G

K

P

E

F

I

D

A

E

P

L

A

Q

A

T

E

E

I

I

M

E

E

V

K

T

T

N

D

K

E

F

F

P

P

D

F

E

Q

F

L

Y

I

E

K

L

K

A

M

K

G

E

K

H

H

N

G

L

L

Y

M

R

G

F

I

Y

P

M

V

P

P

E

E

Q

Q

Y

Y

G

G

W

A

G

G

L

A

S

D

T

V

L

V

E

S

I

Y

M

I

K

L

V

A

Q

I

E

H

E

E

F

I

S

S

R

R

-

I

-

S

G

A

P

A

G

V

G

G

M

V

R

I

M

L

H

S

L

V

H

H

H

T

A

S

A

V

G

G

L

T

N

N

W

P

R

I

I

L

M

Y

D

-

F

F

A

G

Q

N

P

E

E

E

L

Q

K

K

E

M

W

K

A

Y

M

I

P

P

R

N

F

L

Q

A

N

S

K

G

T

D

L

H

F

L

V

G

N

A

F

F

A

A

L
|
L

T
|
T

E

E

K

P

E

H

A

S

G
|
G

S
|
S

G

D

A

A

-

G

D

S

I

L

K

R

T

T

A

A

V

I

R

K

D

K

G

N

D

G

E

K

W

Y

V

L

I

L

N

N

G

G

E

S

K

K

T

I

L
x
F

I

I

S
x
T

H

N

T

G

D

G

C

A

S

A

D

D

G

I

T

Y

Y

I

L

T

I

F

T

A

L

L

T

T

N

A

P

P

E

D

A

Q

D

G

K

R

D

H

K

G

R

-

L

I

T

S

A

A

F

F

F

I

V

V

P

E

T

K

D

N

T

T

P

P

G

G

Y

F

E

T

I

V

V

G

D

K

M

K

P

E

H

R

M

K

M

L

G

G

C

L

R

Y

G

G

A

S

G

N

H

T

A

T

G

E

L

L

R

I

F

F

T

D

D

N

C

A

R

E

V

V

P

P

D

E

K

A

Y

N

R

L

L

L

G

G

E

K

V

E

G

G

E

D

G

G

L

F

H

H

V

I

A

A

M

M

Y

A

S

N

L

L

G

N

L

V

S
x
G

R

R

L

I

H

G

I

I

A

A

D

A

S

Q

N

A

L

L

G

G

M

I

A

A

Q

E

R

A

M

A

L

L

E

E

M

H

S

A

I

V

A

D

R

Y

A

A

K

K

E

Q

R
|
R

I

V

T
x
Q

F
|
F

G

G

K

R

P

P

L
x
I

I

A

K

A

R
x
N

Q

Q

A

G

I
|
I

Q

S

T

F

M

K

I

L

A

A

E

D

S

M

G

A

K

T

W

R

I

A

Y

E

L

A

R

R

S

H

A

L

I

V

H

Y

D

H

A

A

-

D

-

L

-

H

R

N

R

R

Y

L

D

N

A

C

G

G

E

K

D

-

P

-

M

-

T

-

Q

E

A

A

S

S

L

M

C

A

K

K

-

Q

L

F

A

A

S

S

I

D

D

A

A

A

V

V

K

K

I

A

V

L

S

-

D

-

N

D

I

A

L

V

E
x
Q

I
|
I

L

Y

G

G

G
|
G

I

Y

G

G

Y

Y

F

M

E

K

E

D

C

Y

E

-

Y

-

G

-

P

P

A

V

E

E

R

R

L

L

Y

L

R

R

D

D

C

A

R

K

A

V

M

T

W

Q

L

I

E
x
Y

E
|
E

G

G

P
x
T

P

N

S
x
E

V

I

Q

Q

R

R

T

L

T

I

A

I

A

S

R
x
K

G

Y

L

L

I

L

active site: L122 (= L128), T123 (= T129), G239 (≠ S245), E358 (= E369), K370 (≠ R381)
binding flavin-adenine dinucleotide: L122 (= L128), T123 (= T129), G128 (= G134), S129 (= S135), F153 (≠ L158), T155 (≠ S160), R265 (= R271), Q267 (≠ T273), F268 (= F274), I272 (≠ L278), N275 (≠ R281), I278 (= I284), Q331 (≠ E339), I332 (= I340), G335 (= G343), Y357 (≠ E368), T360 (≠ P371), E362 (≠ S373)

1egcA Structure of t255e, e376g mutant of human medium chain acyl-coa dehydrogenase complexed with octanoyl-coa (see paper)
31% identity, 95% coverage: 10:381/393 of query aligns to 9:379/387 of 1egcA

query
sites
1egcA

E

E

E

Q

F

Q

Q

K

Q

E

Y

F

L

Q

M

A

E

T

I

A

R

R

E

K

L

F

A

A

Q

R

G

E

P

E

F

I

D

I

E

P

L

V

Q

A

T

A

E

E

I

Y

E

D

V

K

T

T

N

G

K

E

F

Y

P

P

D

V

E

P

F

L

Y

I

E

R

L

R

A

A

K

W

E

E

H

L

N

G

L

L

Y

M

R

N

F

T

Y

H

M

I

P

P

E

E

Q

N

Y

C

G

G

W

L

G

G

L

L

S

G

T

T

L

F

E

D

I

A

M

C

K

L

V

I

Q

S

E

E

F

L

S

A

R

Y

G

G

P

C

G

T

G

G

M

V

R

Q

M

T

H

A

L

I

H
x
E

H

G

A

N

A

S

G
x
L

L

G

N

Q

W

M

R

P

I

I

M

I

D

I

D

A

F

G

A

N

Q

D

P

Q

E

Q

L

K

K

K

E

K

W

Y

A

-

M

L

P

G

R

R

F

M

Q

T

N

E

K

E

T

P

L

L

F

M

V

C

N

A

F
x
Y

A

C

L
x
V

T
|
T

E

E

K

P

E

G

A

A

G
|
G

S
|
S

G

D

-
x
V

A

A

D

G

I

I

K

K

T

T

T

K

A

A

V

E

R

K

D

K

G

G

D

D

E

E

W

Y

V

I

I

I

N

N

G

G

E

Q

K

K

T

M

L
x
W

I

I

S
x
T

H

N

T

G

D

G

C

K

S

A

D

N

G

W

T

Y

Y

F

L

L

I

L

T

A

L

R

T

S

N

D

P

P

E

D

-

P

-

K

A

A

D

P

K

A

D
x
N

K

K

R

A

L

F

T

T

A

G

F

F

F

I

V

V

P

E

T

A

D

D

T

T

P

P

G

G

Y

I

E

Q

I

I

V

G

D

R

M

K

P

E

H

L

M

N

M

M

G

G

C

Q

R

R

G

C

A

S

G

D

H

T

A

R

G

G

L

I

R

V

F

F

T

E

D

D

C

V

R

K

V

V

P

P

D

K

K

E

Y

N

R

V

L

L

G

I

E

G

V

D

G

G

E

A

G

G

L
x
F

H

K

V

V

A

A

M
|
M

Y

G

S

A

L
x
F

G
x
D

L

K

S
x
E

R
|
R

L

P

H

V

I

V

A

A

D

A

S

G

N

A

L

V

G

G

M

L

A

A

Q

Q

R

R

M

A

L

L

E

D

M

E

S

A

I

T

A

K

R

Y

A

A

K

L

E

E

R
|
R

I

K

T
|
T

F
|
F

G

G

K

K

P

L

L
|
L

I

V

K

E

R
x
H

Q

Q

A

A

I
|
I

Q

S

T

F

M

M

I

L

A

A

E

E

S

M

G

A

K

M

W

K

I

V

Y

E

L

L

L

A

R

R

S

M

A

S

I

Y

H

Q

D

R

A

A

R

W

R

E

Y

V

D

D

A

S

G

G

E

R

D

R

P

N

M

T

T

Y

Q

Y

A

A

S

S

L

I

C

A

K

K

L

A

A

F

S

A

I

G

D

D

A

I

V

A

K

N

I

Q

V

L

S

A

D

T

N

D

I

A

L

V

E
x
Q

I
|
I

L

L

G

G

G
|
G

I

N

G

G

Y

F

F

-

E

-

E

N

C

T

E

E

Y

Y

G

-

P

P

A

V

E

E

R

K

L

L

Y

M

R

R

D

D

C

A

R

K

A
x
I

M

Y

W

Q

L
x
I

E
x
Y

E
x
G

G
|
G

P
x
T

P

S

S
x
Q

V

I

Q

Q

R

R

T

L

T

I

A

V

A

A

R
|
R

active site: V126 (≠ L128), T127 (= T129), E246 (≠ S245), G367 (≠ E369), R379 (= R381)
binding octanoyl-coenzyme a: E90 (≠ H91), L94 (≠ G95), Y124 (≠ F126), S133 (= S135), V135 (vs. gap), N182 (≠ D181), F236 (≠ L235), M240 (= M239), F243 (≠ L242), D244 (≠ G243), R247 (= R246), Y366 (≠ E368), G367 (≠ E369), G368 (= G370)
binding flavin-adenine dinucleotide: Y124 (≠ F126), V126 (≠ L128), T127 (= T129), G132 (= G134), S133 (= S135), W157 (≠ L158), T159 (≠ S160), R272 (= R271), T274 (= T273), F275 (= F274), L279 (= L278), H282 (≠ R281), I285 (= I284), Q340 (≠ E339), I341 (= I340), G344 (= G343), I362 (≠ A364), I365 (≠ L367), Y366 (≠ E368), T369 (≠ P371), Q371 (≠ S373)

6fahD Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
30% identity, 89% coverage: 29:376/393 of query aligns to 25:369/379 of 6fahD

query
sites
6fahD

E

E

L

I

Q

L

T

D

E

E

I

V

E

E

V

E

T

S

N

G

K

E

F

F

P

P

D

Q

E

E

F

I

Y

L

E

D

L

K

A

M

K

A

E

K

H

F

N

G

L

F

Y

F

R

G

F

I

Y

K

M

I

P

P

E

K

Q

S

Y

L

G

G

W

S

G

G

L

G

S

D

T

H

L

M

E

S

I

Y

M

V

K

I

V

C

Q

M

E

E

F

F

S

A

R

R

G

V

P

S

G

G

G

V

M

A

R

S

M

V

H

Y

L

L

H

S

A

P

A

N

G

S

L

L

-

A

N

G

W

G

R

P

I

L

M

L

D

L

D

S

F

G

A

T

Q

E

P

E

E

Q

L

I

K

E

E

K

W

Y

A

L

M

K

P

P

R

I

F

I

Q

T

N

G

K

K

T

K

L

K

F

L

V

A

N

-

F
|
F

A

A

L
|
L

T
|
T

E

E

K

P

E

G

A

A

G

G

S

S

G

D

A

A

D

G

-

G

I

M

K

S

T

T

A

A

V

V

R

D

D

M

G

G

D

D

E

Y

W

Y

V

L

I

L

N

N

G

G

E
x
R

K

K

T

T

L
x
F

I

I

S
x
T

H

M

T

A

D

P

C

L

S

C

D

D

G

D

T

A

Y

V

L

I

I

Y

T

A

L

K

T

T

N

D

P

M

E

-

A

S

D

K

K

G

D

T

K

R

R

G

L

I

T

S

A

A

F

F

F

I

V

V

P

D

T

L

D

K

T

S

P

E

G

G

Y

V

E

S

I

M

V

G

D

K

M

N

P
x
E

H

H

M

K

M

M

G

G

C

L

R

I

G

G

A

C

G

A

H

T

A

S

G

D

L

I

R

I

F

M

T

E

D

D

C

V

R

K

V

V

P

P

D

K

K

E

Y

N

R

R

L

L

G

G

E

E

V

V

G

N

E

K

G

G

L

F

H

S

V

N

A

A

M

M

Y

K

S

T

L

L

G

D

L

V

S
x
G

R

R

L

L

H

G

I

V

A

A

D

S

S

Q

N

S

L

I

G

G

M

V

A

A

Q

Q

R

G

M

A

L

L

E

D

M

E

S

A

I

I

A

K

R

Y

A

A

K

K

E

E

R
|
R

I

K

T
x
Q

F
|
F

G

G

K

K

P

R

L
x
I

I

A

K

D

R
x
F

Q

Q

A

A

I

I

Q

A

T

F

M

M

I

I

A

A

E

D

S

M

G

A

K

T

W

K

I

L

Y

E

L

A

R

K

S

L

A

L

I

V

H

Y

D

N

A

A

R

S

R

L

Y

M

D

D

A

N

G

K

E

K

D

N

P

A

M

T

T

K

Q

E

A

A

S

S

L

M

C

A

K

K

L

F

A

Y

S

A

I

S

D

E

A

I

V

C

K

N

I

E

V

I

S

C

D

A

N

K

I

A

L

V

E
x
Q

I
|
I

L

H

G

G

G
|
G

I

Y

G

G

Y

Y

F

I

E

K

E

E

C

Y

E

K

Y

-

G

-

P

-

A

V

E

E

R

R

L

M

Y

Y

R

R

D

D

C

C

R

R

A

V

M

F

W

T

L

I

E
x
Y

E

E

G

G

P
x
T

P

S

S
x
Q

V

V

Q

Q

R

Q

active site: L124 (= L128), T125 (= T129), G241 (≠ S245)
binding flavin-adenine dinucleotide: F122 (= F126), L124 (= L128), T125 (= T129), R152 (≠ E155), F155 (≠ L158), T157 (≠ S160), E198 (≠ P202), R267 (= R271), Q269 (≠ T273), F270 (= F274), I274 (≠ L278), F277 (≠ R281), Q335 (≠ E339), I336 (= I340), G339 (= G343), Y361 (≠ E368), T364 (≠ P371), Q366 (≠ S373)

Sites not aligning to the query:

active site: 374

3mdeA Crystal structures of medium chain acyl-coa dehydrogenase from pig liver mitochondria with and without substrate (see paper)
30% identity, 95% coverage: 10:381/393 of query aligns to 8:378/385 of 3mdeA

query
sites
3mdeA

E

E

E

Q

F

Q

Q

K

Q

E

Y

F

L

Q

M

A

E

T

I

A

R

R

E

K

L

F

A

A

Q

R

G

E

P

E

F

I

D

I

E

P

L

V

Q

A

T

A

E

E

I

Y

E

D

V

R

T

T

N

G

K

E

F

Y

P

P

D

V

E

P

F

L

Y

L

E

K

L

R

A

A

K

W

E

E

H

L

N

G

L

L

Y

M

R

N

F

T

Y

H

M

I

P

P

E

E

Q

S

Y

F

G

G

W

L

G

G

L

L

S

G

T

I

L

I

E

D

I

S

M

C

K

L

V

I

Q

T

E

E

F

L

S

A

R

Y

G

G

P

C

G

T

G

G

M

V

R

Q

M
x
T

H

A

L

I

H
x
E

-

A

H

N

A

T

A
x
L

G

G

L

Q

N

V

W

P

R

L

I

I

M

I

D

G

F

N

A

Y

Q

Q

P

Q

E

Q

L

K

K

K

E

-

W

-

A

Y

M

L

P

G

R

R

F

M

Q

T

N

E

K

E

T

P

L

L

F

M

V

C

N

A

F
x
Y

A

C

L
x
V

T
|
T

E

E

K

P

E

G

A

A

G
|
G

S
|
S

G

D

-
x
V

A

A

D

G

I

I

K

K

T

T

T

K

A

A

V

E

R

K

D

K

G

G

D

D

E

E

W

Y

V

I

I

I

N

N

G

G

E

Q

K

K

T

M

L
x
W

I
|
I

S
x
T

H

N

T

G

D

G

C

K

S

A

D

N

G

W

T

Y

Y

F

L

L

I

L

T

A

L

R

T

S

N

D

P

P

E

D

-

P

-

K

A

A

D

P

K

A

D
x
S

K

K

R

A

L

F

T

T

A

G

F

F

F

I

V

V

P

E

T

A

D

D

T

T

P

P

G

G

Y

V

E

Q

I

I

V

G

D

R

M

K

P

E

H

I

M

N

M

M

G

G

C

Q

R

R

G

C

A

S

G

D

H

T

A

R

G

G

L

I

R

V

F

F

T

E

D

D

C

V

R

R

V

V

P

P

D

K

K

E

Y

N

R

V

L

L

G

T

E

G

V

E

G

G

E

A

G

G

L
x
F

H

K

V

I

A

A

M
|
M

Y

G

S

T

L
x
F

G

D

L

K

S
x
T

R

R

L

P

H

P

I

V

A

A

D

A

S

G

N

A

L

V

G

G

M

L

A

A

Q

Q

R

R

M

A

L

L

E

D

M

E

S

A

I

T

A

K

R

Y

A

A

K

L

E

E

R
|
R

I

K

T
|
T

F
|
F

G

G

K

K

P

L

L
|
L

I

A

K

E

R
x
H

Q

Q

A

G

I

I

Q

S

T

F

M

L

I

L

A

A

E

D

S

M

G

A

K

M

W

K

I

V

Y

E

L

L

L

A

R

R

S

L

A

S

I

Y

H

Q

D

R

A

A

R

W

R

E

Y

I

D

D

A

S

G

G

E

R

D
x
R

P

N

M

T

T

Y

Q

Y

A

A

S

S

L

I

C

A

K

K

L

A

A

Y

S

A

I

A

D

D

A

I

V

A

K

N

I

Q

V

L

S

A

D

T

N

D

I

A

L

V

E
x
Q

I
x
V

L

F

G

G

G
|
G

I

N

G

G

Y

F

F

-

E

-

E

N

C

T

E

E

Y

Y

G

-

P

P

A

V

E

E

R

K

L

L

Y

M

R

R

D

D

C

A

R

K

A
x
I

M

Y

W

Q

L

I

E
x
Y

E
|
E

G
|
G

P
x
T

P

A

S
x
Q

V

I

Q

Q

R

R

T

I

A

I

A

A

R
|
R

active site: V125 (≠ L128), T126 (= T129), T245 (≠ S245), E366 (= E369), R378 (= R381)
binding octanoyl-coenzyme a: T86 (≠ M88), E89 (≠ H91), L93 (≠ A94), S132 (= S135), V134 (vs. gap), S181 (≠ D181), F235 (≠ L235), M239 (= M239), F242 (≠ L242), R314 (≠ D314), Y365 (≠ E368), E366 (= E369), G367 (= G370)
binding flavin-adenine dinucleotide: Y123 (≠ F126), V125 (≠ L128), T126 (= T129), G131 (= G134), S132 (= S135), W156 (≠ L158), I157 (= I159), T158 (≠ S160), R271 (= R271), T273 (= T273), F274 (= F274), L278 (= L278), H281 (≠ R281), Q339 (≠ E339), V340 (≠ I340), G343 (= G343), I361 (≠ A364), T368 (≠ P371), Q370 (≠ S373)

Query Sequence

>WP_017178764.1 NCBI__GCF_000295095.1:WP_017178764.1
MITSKPKNDEEFQQYLMEIRELAQGPFDELQTEIEVTNKFPDEFYELAKEHNLYRFYMPE
QYGGWGLSTLEIMKVQEEFSRGPGGMRMHLHHAAGLNWRIMDDFAQPELKEWAMPRFQNK
TLFVNFALTEKEAGSGADIKTTAVRDGDEWVINGEKTLISHTDCSDGTYLITLTNPEADK
DKRLTAFFVPTDTPGYEIVDMPHMMGCRGAGHAGLRFTDCRVPDKYRLGEVGEGLHVAMY
SLGLSRLHIADSNLGMAQRMLEMSIARAKERITFGKPLIKRQAIQTMIAESGKWIYLLRS
AIHDAARRYDAGEDPMTQASLCKLASIDAVKIVSDNILEILGGIGYFEECEYGPAERLYR
DCRAMWLEEGPPSVQRTTAARGLITSGGDLWNS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory