SitesBLAST

Q84P21 Peroxisomal OPC-8:0-CoA ligase 1; 4-coumarate--CoA ligase isoform 9; At4CL9; 4-coumarate--CoA ligase-like 5; EC 6.2.1.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 92% coverage: 44:549/551 of query aligns to 53:536/546 of Q84P21

query
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Q84P21

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K530 (= K543) mutation to N: Lossed enzymatic activity.

P9WQ37 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
30% identity, 91% coverage: 49:551/551 of query aligns to 30:495/503 of P9WQ37

query
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P9WQ37

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K172 (= K222) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Slight increase of susceptibility to proteolysis.
R195 (= R248) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
R197 (≠ K250) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-195 and A-244.
V209 (= V262) mutation to D: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced. Slight increase of susceptibility to proteolysis.
A211 (= A264) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.
T214 (≠ V267) mutation to W: Shows a marked decrease in the activity with lauric and palmitic acid (C12 and C16 fatty acid) with a simultaneous increase in the activity with caprylic acid (C8 fatty acid).
R244 (≠ K299) mutation to A: Alteration of the strength of the membrane binding; when associated with A-17; A-195; A-195 and A-197.
A302 (≠ G358) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.; mutation to W: Does not show activity with small, medium or long acyl chains.
W377 (= W434) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding. No significant change in the total expression level, however the cytoplasmic expression is low. Slight increase of susceptibility to proteolysis.
D382 (= D439) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced.
R397 (= R454) mutation to A: Reduction of binding affinity for fatty acids.
S404 (≠ V461) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding.
G406 (= G463) mutation to L: No effect on the formation of acyl-adenylate intermediate. However, it shows very poor catalytic efficiency to form acyl-CoA.
K487 (= K543) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Reduction of binding affinity for ATP.

Sites not aligning to the query:

9 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-195; A-197 and A-244.
17 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.

Q67W82 4-coumarate--CoA ligase 4; 4CL 4; Os4CL4; (E)-ferulate--CoA ligase; 4-coumaroyl-CoA synthase 4; Protein RESISTANCE TO ALUMINUM 1; EC 6.2.1.12; EC 6.2.1.34 from Oryza sativa subsp. japonica (Rice) (see paper)
29% identity, 99% coverage: 4:550/551 of query aligns to 18:542/559 of Q67W82

query
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Q67W82

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G395 (= G406) mutation to R: In ral1; reduced lignin content and increased accumulation of 4-coumarate and ferulate in roots; confers increased tolerance to aluminum.

Q9S725 4-coumarate--CoA ligase 2; 4CL 2; 4-coumarate--CoA ligase isoform 2; At4CL2; 4-coumaroyl-CoA synthase 2; Caffeate--CoA ligase; EC 6.2.1.12; EC 6.2.1.- from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
27% identity, 99% coverage: 4:550/551 of query aligns to 22:547/556 of Q9S725

query
sites
Q9S725

V

I

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K211 (= K222) mutation to S: Drastically reduces the activity.
M293 (≠ T304) mutation M->A,P: Affects the substrate specificity.
K320 (≠ V331) mutation K->L,A: Affects the substrate specificity.
E401 (= E407) mutation to Q: Slighlty reduces the substrate specificity.
C403 (≠ Q409) mutation to A: Significantly reduces the substrate specificity.
R449 (= R454) mutation to Q: Drastically reduces the activity.
K457 (≠ S462) mutation to S: Drastically reduces the activity.
K540 (= K543) mutation to N: Abolishes the activity.

3ni2A Crystal structures and enzymatic mechanisms of a populus tomentosa 4- coumarate:coa ligase (see paper)
27% identity, 95% coverage: 31:551/551 of query aligns to 26:527/528 of 3ni2A

query
sites
3ni2A

Q

E

T

N

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-

A

-

K

N

E

P

P

Y

F

D

D

L

I

H

K

E

P

Y

-

N

-

G

G

S

A

I

C

G

G

L

T

P

V

V

V

S

R

S

N

T

A

D

E

V

M

R

K

I

I

I

V

D

D

D

P

E

E

G

T

G

G

V

A

L

S

T

L

K

P

P

R

G

N

A

Q

V

P

G

G

E

E

M

I

Q

C

V

I

R

R

G

G

P

D

Q

Q

V

I

M

M

Q

K

G

G

Y

Y

W

L

Q

N

R

D

P

P

Q

E

D

A

T

T

A

S

E

R

V

T

I

I

-

D

T

K

D

E

G

G

W

W

L

L

N

H

T

T

G

G

D
|
D

I

I

A

G

R

Y

M

I

D

D

E

D

G

D

F

E

F

L

Y

F

I

I

V

V

D

D

R

R

K

L

K
|
K

D

E

M

L

I

I

L
x
K

V

Y

S

K

G

G

F

F

N
x
Q

V

V

F

A

P

P

N

A

E

E

I

L

E

E

D

A

V

L

L

L

T

I

M

A

N

H

D

P

N

E

I

I

L

S

E

D

A

A

A

V

I

V

G

G

V

L

P

K

H

D

E

E

S

D

S

A

G

G

E

E

T

V

V

P

K

V

I

A

F

F

V

V

K

K

-

S

-

E

K

K

G

S

E

Q

I

A

S

T

K

E

E

D

E

E

I

I

I

K

A

Q

H

Y

C

I

R

S

E

K

H

Q

L

V

T

I

A

F

Y

Y

K

K

I

R

P

I

R

K

I

R

I

V

E

F

F

F

R

I

D

E

E

A

L

I

P

P

K

K

S

A

N

P

V

S

G

G

K
|
K

I

I

L

L

R

R

R

K

E

N

L

L

R

K

D

E

active site: S182 (≠ T214), S202 (≠ N234), H230 (= H261), T329 (= T360), E330 (= E361), K434 (≠ L460), Q439 (≠ N465), K519 (= K543)
binding 5'-O-{(S)-hydroxy[3-(4-hydroxyphenyl)propoxy]phosphoryl}adenosine: Y232 (≠ F263), S236 (≠ N268), G302 (= G334), A303 (≠ M335), P304 (≠ A336), G325 (= G356), G327 (= G358), T329 (= T360), P333 (= P364), V334 (≠ L365), D413 (= D439), K430 (= K456), K434 (≠ L460), Q439 (≠ N465)

3a9vA Crystal structures and enzymatic mechanisms of a populus tomentosa 4- coumarate--coa ligase (see paper)
27% identity, 95% coverage: 31:551/551 of query aligns to 26:527/528 of 3a9vA

query
sites
3a9vA

Q

E

T

N

V

L

Q

S

N

N

Y

H

A

S

D

S

N

K

P

P

A

C

Y

L

V

I

N

N

-

G

-

A

M

N

G

G

C

D

S

V

L

Y

T

T

Y

Y

G

A

E

D

I

V

D

E

E

L

K

T

S

A

R

R

A

R

F

V

A

A

A

S

Y

G

L

L

Q

-

N

N

E

K

L

I

K

G

L

I

T

Q

K

Q

G

G

D

D

R

V

V

I

A

M

L

L

M

F

M

L

P

P

N

S

L

S

L

P

Q

E

Y

F

P

V

I

L

A

A

L

F

F

L

G

G

A

A

L

S

R

H

A

R

G

G

M

A

V

I

I

I

V

T

N

A

V

A

N

N

P

P

L

F

Y

S

T

T

P

P

R

A

E

E

L

L

K

A

H

K

Q

H

L

A

N

K

D

A

S

S

G

R

A

A

K

K

A

L

I

L

V

I

I

T

I

Q

S

A

N

C

F

Y

A

Y

S

E

V

K

L

V

E

K

K

D

V

F

I

A

K

R

D

E

S

S

P

D

V

V

E

K

H

V

V

M

I

C

L

V

T

D

Q

S

L

A

G

P

D

D

L

-

F

-

S

-

P

-

V

-

K

-

G

-

A

-

I

-

T

-

N

-

L

-

V

-

K

-

Y

-

V

-

K

-

M

-

V

-

P

-

K

-

F

-

N

-

L

-

P

-

N

G

A

C

I

L

P

H

F

F

N

S

R

E

V

-

L

L

S

T

K

Q

G

A

R

D

S

E

L

N

Q

E

F

A

T

P

K

Q

V

V

E

D

A

I

G

S

F

P

D

D

I

V

A

V

F

A

L

L

Q

P

Y

Y

T
x
S

G

S

G

G

T

T

G

G

V

L

S

P

K

K

G

G

A

V

V

M

L

L

T

T

H

H

K

K

N

G

M

L

L

I

A

T

N
x
S

V

V

L

A

Q

Q

-

Q

A

V

E

D

G

G

A

D

Y

N

G

P

S

N

I

L

I

Y

D

F

R

H

G

S

K

E

E

D

T

V

V

I

I

L

T

C

A

V

L

L

P

P

L

M

Y

F

H
|
H

V

I

F

Y

A

A

L

L

T

N

V

-

N

S

G

I

L

M

L

L

F

C

F

G

L

L

G

R

G

V

G

G

K

A

N

P

I

I

L

L

I

I

T

M

N

P

P

K

R

F

D

E

L

I

P

G

A

S

L

L

I

L

K

G

E

L

I

I

S

E

D

K

H

Y

K

K

P

V

T

S

A

I

I

A

T

P

G

V

V

V

N

P

T

P

L

V

F

M

N

M

A

S

L

I

V

A

N

K

D

S

D

P

S

D

F

L

A

D

N

K

I

H

D

D

F

L

S

S

A

S

L

L

K

R

L

M

S

I

V

K

G

S

G

G

G
|
G

M
x
A

A
x
P

V

L

Q

G

R

K

S

E

V

L

A

E

E

D

K

T

W

V

K

R

-

A

K

K

I

F

T

P

G

Q

C

A

H

R

L

L

L

G

E

Q

G

G

Y
|
Y

G
|
G

L
x
M

T
|
T

E
|
E

C

A

S

G

P

P

L

V

V

L

T

A

V

M

-

C

-

L

-

A

-

F

-

A

-

K

N

E

P

P

Y

F

D

D

L

I

H

K

E

P

Y

-

N

-

G

G

S

A

I

C

G

G

L

T

P

V

V

V

S

R

S

N

T

A

D

E

V

M

R

K

I

I

I

V

D

D

D

P

E

E

G

T

G

G

V

A

L

S

T

L

K

P

P

R

G

N

A

Q

V

P

G

G

E

E

M

I

Q

C

V

I

R

R

G

G

P

D

Q

Q

V

I

M

M

Q

K

G

G

Y

Y

W

L

Q

N

R

D

P

P

Q

E

D

A

T

T

A

S

E

R

V

T

I

I

-

D

T

K

D

E

G

G

W

W

L

L

N

H

T

T

G

G

D
|
D

I

I

A

G

R

Y

M

I

D

D

E

D

G

D

F

E

F

L

Y

F

I

I

V

V

D

D

R

R

K

L

K
|
K

D

E

M

L

I

I

L
x
K

V

Y

S

K

G

G

F

F

N
x
Q

V

V

F

A

P

P

N

A

E

E

I

L

E

E

D

A

V

L

L

L

T

I

M

A

N

H

D

P

N

E

I

I

L

S

E

D

A

A

A

V

I

V

G

G

V

L

P

K

H

D

E

E

S

D

S

A

G

G

E

E

T

V

V

P

K

V

I

A

F

F

V

V

K

K

-

S

-

E

K

K

G

S

E

Q

I

A

S

T

K

E

E

D

E

E

I

I

I

K

A

Q

H

Y

C

I

R

S

E

K

H

Q

L

V

T

I

A

F

Y

Y

K

K

I

R

P

I

R

K

I

R

I

V

E

F

F

F

R

I

D

E

E

A

L

I

P

P

K

K

S

A

N

P

V

S

G

G

K
|
K

I

I

L

L

R

R

R

K

E

N

L

L

R

K

D

E

active site: S182 (≠ T214), S202 (≠ N234), H230 (= H261), T329 (= T360), E330 (= E361), K434 (≠ L460), Q439 (≠ N465), K519 (= K543)
binding adenosine monophosphate: H230 (= H261), G302 (= G334), A303 (≠ M335), P304 (≠ A336), Y326 (= Y357), G327 (= G358), M328 (≠ L359), T329 (= T360), D413 (= D439), K430 (= K456), K434 (≠ L460), Q439 (≠ N465)

3r44A Mycobacterium tuberculosis fatty acyl coa synthetase (see paper)
29% identity, 91% coverage: 49:551/551 of query aligns to 33:495/502 of 3r44A

query
sites
3r44A

L

M

T

T

Y

Y

G

A

E

Q

I

M

D

N

E

A

K

L

S

A

R

N

A

R

F

C

A

A

A

D

Y

V

L

L

Q

-

N

T

E

A

L

L

K

G

L

I

T

A

K

K

G

G

D

D

R

R

V

V

A

A

L

L

M

L

M

M

P

P

N

N

L

S

L

V

Q

E

Y

F

P

C

I

C

A

L

L

F

F

Y

G

G

A

A

L

A

R

K

A

L

G

G

M

A

V

V

I

A

V

V

N

P

V

I

N

N

P

T

L

R

Y

L

T

A

P

A

R

P

E

E

L

V

K

S

H

F

Q

I

L

L

N

S

D

D

S

S

G

G

A

S

K

K

A

V

I

V

V

I

I

Y

I

G

S

A

N

P

F

S

A

A

S

P

V

V

L

I

E

D

K

A

V

I

I

R

K

A

D

Q

S

A

P

D

V

-

E

-

H

-

V

-

I

-

L

-

T

-

Q

-

L

-

G

-

D

-

L

-

F

-

S

-

P

-

V

-

K

-

G

-

A

-

I

-

T

-

N

-

L

-

V

-

K

-

Y

-

V

-

K

-

M

-

V

-

P

P

K

P

F

G

N

T

L

V

P

T

N

D

A

W

I

I

P

G

F

A

N

D

R

S

V

L

L

A

S

E

K

R

G

L

R

R

S

S

L

A

Q

A

F

A

T

D

K

E

-

P

-

A

V

V

E

E

A

C

G

G

F

G

D

D

I

N

A

L

F

F

L

I

Q

M

Y

Y

T
|
T

G

-

T

-

T

S

G

G

V

H

S

P

K

K

G

G

A

V

V

V

L

H

T

T

H

H

K
x
E

N

S

M

-

L

-

A

-

N

-

V

V

L
x
H

Q

S

A
|
A

E

A

G

S

A

S

Y

W

G

A

S

S

I

T

I

I

D

D

-

V

R

R

G

Y

K

R

E

D

T

R

V

L

I

L

T

L

A

P

L

L

P

P

L

M

Y

F

H
|
H

V

V

F

A

A

A

L

L

T

T

V

T

N

-

G

-

L

-

L

V

F

I

F

F

L

S

G

A

G

M

G

R

K

G

N

V

I

T

L

L

I

I

T

S

N

M

P

P

R

Q

-

F

D

D

L

A

P

T

A

K

L

V

I

W

K

S

E

L

I

I

S

V

D

E

H

E

K

R

P

V

T

C

A

I

I

G

T

G

G

A

V

V

N

P

T

A

L

I

F

L

N

N

A

F

L

M

V

R

N

Q

D

V

D

P

S

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F

F

A

A

N

E

I

L

D

D

F

A

S

P

A

D

L

F

K

R

L

Y

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V

I

G

T

G

G

M

A

A

P

V

M

Q

P

R

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S

A

V

L

A

I

E

K

K

I

W

Y

K

A

I

-

T

K

G

N

C

I

H

E

L

V

E

Q

G

G

Y

Y

G

A

L

L

T
|
T

E
|
E

-

S

C

C

S

G

P

G

L

G

V

T

T

L

V

L

N

L

P

S

Y

E

D

D

L

A

H

L

E

R

Y

K

N

A

G

G

S

S

I

A

G

G

L

R

P

A

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T

S

M

S

F

T

T

D

D

V

V

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A

I

V

I

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D

G

D

D

E

D

G

G

G

-

V

V

L

I

T

R

K

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P

H

G

G

A

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V

-

G

G

E

E

M

V

Q

V

V

I