SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_017221119.1 NCBI__GCF_000276805.1:WP_017221119.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
70% identity, 99% coverage: 3:498/501 of query aligns to 2:500/501 of P04983

query
sites
P04983

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K43 (= K44) mutation to R: Loss of transport.

1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
32% identity, 44% coverage: 2:223/501 of query aligns to 1:231/254 of 1g6hA

query
sites
1g6hA

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binding adenosine-5'-diphosphate: F14 (= F15), F17 (≠ V18), N39 (= N40), G40 (= G41), G42 (= G43), K43 (= K44), S44 (= S45), T45 (= T46)

1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
32% identity, 44% coverage: 2:223/501 of query aligns to 1:231/253 of 1g9xB

query
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1g9xB

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binding adenosine-5'-diphosphate: F14 (= F15), F17 (≠ V18), G40 (= G41), S41 (≠ A42), G42 (= G43), K43 (= K44), S44 (= S45), T45 (= T46)
binding magnesium ion: S44 (= S45), E176 (= E168)

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
30% identity, 45% coverage: 4:229/501 of query aligns to 1:223/240 of 6mjpA

query
sites
6mjpA

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binding calcium ion: E111 (≠ I114), D115 (≠ Q118)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
30% identity, 43% coverage: 5:221/501 of query aligns to 4:222/648 of P75831

query
sites
P75831

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A

T

T

F

L

D

D

G

A

P

D

R

A

H

L

S

A

Q

Q

-

L

-

R

E

R

V

E

G

H

I

F

S

G

I

F

I

I

H

F

Q

Q

E

R

L

Y

N

H

L

L

I

L

P

S

E

H

L

L

T

T

I

A

A

E

E

Q

N

N

I

V

Y

-

L

-

G

-

R

-

E

E

T

V

T

P

N

A

A

V

F

Y

G

A

G

G

I

L

K

E

W

R

T

K

Q

Q

M

R

F

L

A

L

D

R

A

A

D

Q

A

E

L

L

Q

Q

R

R

L

L

N

G

V

L

K

E

H

D

S

R

S

T

R

E

Q

Y

L

Y

L

P

G

A

E

Q

L

L

S

S

L

G

G

G

E

Q

Q

Q

M

R

V

V

E

S

I

I

A

A

K

R

A

A

L

L

S

M

F

N

K

G

S

G

Q

Q

V

V

I

I

V

L

M

A

D
|
D

E

E

P

P

T

T

D

G

A

A

L

L

T

D

E

S

S

H

E

S

T

G

K

E

S

E

L

V

F

M

K

A

V

I

I

L

N

H

E

Q

L

L

R

R

N

D

E

R

G

G

C

H

G

T

I

V

V

I

Y

I

I

V

S

T

H

H

R

D

L

-

K

P

E

Q

I

V

F

A

E

A

I

Q

C

A

D

E

D

R

I

V

T

I

V

E

L

I

R

R

D

D

G

G

K

E

F

I

I

V

K47 (= K44) mutation to L: Lack of activity.
D169 (= D167) mutation to N: Lack of activity.

1ji0A Crystal structure analysis of the abc transporter from thermotoga maritima
29% identity, 47% coverage: 5:237/501 of query aligns to 6:234/240 of 1ji0A

query
sites
1ji0A

I

V

L

L

K

E

L

V

S

Q

G

S

I

L

E

H

K

V

S

Y

F
x
Y

P

G

G

A

V

I

K

H

A

A

L

I

D

K

N

G

A

I

C

D

L

L

N

K

V

V

Y

P

P

R

G

G

K

Q

V

I

M

V

A

T

L

L

L

I

G

G

E

A

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

L

K

S

V

A

L

I

T

A

G

G

I

L

Y

V

H

R

M

A

D

Q

K

K

G

G

S

K

I

I

N

I

Y

F

Q

N

G

G

S

Q

D

D

V

I

T

T

F

-

D

N

G

K

P

P

R

A

H

H

S

V

-

I

Q

N

E

R

V

M

G

G

I

I

S

A

I

L

I

V

H

P

Q
x
E

E

G

L

R

N

R

L

I

I

F

P

P

E

E

L

L

T

T

I

V

A

Y

E

E

N

N

I

L

Y

M

L

M

G

G

R

A

E

Y

T

N

T

R

N

K

A

D

F

K

G

E

G

G

I

I

K

K

-

R

-

D

-

L

-

E

W

W

T

-

Q

-

M

I

F

F

A

S

D

L

A

F

D

P

A

R

L

L

L

K

Q

E

R

R

L

L

N

-

V

-

K

-

H

-

S

-

R

K

Q

Q

L

L

L

G

G

G

E

T

L

L

S

S

L

G

G

G

E

E

Q

Q

M

M

V

L

E

A

I

I

A

G

K

R

A

A

L

L

S

M

F

S

K

R

S

P

Q

K

V

L

I

L

V

M

M

M

D

D

E
|
E

P

P

T

S

D

L

A

G

L

L

T

A

E

P

S

I

E

L

T

V

K

S

S

E

L

V

F

F

K

E

V

V

I

I

N

Q

E

K

L

I

R

N

N

Q

E

E

G

G

C

T

G

T

I

I

V

L

Y

L

I

V

S

E

H

Q

R

N

L

A

K

L

E

G

I

A

F

L

E

K

I

V

C

A

D

H

D

Y

I

G

T

Y

V

V

L

L

R

E

D

T

G

G

K

Q

F

I

I

V

A

L

E

E

I

G

A

K

V

A

T

S

D

E

I

L

D

L

E

D

D

N

G

E

L

M

I

V

E

R

K

K

binding adenosine-5'-triphosphate: Y16 (≠ F15), G42 (= G41), G44 (= G43), K45 (= K44), T46 (≠ S45), T47 (= T46), E88 (≠ Q87), E165 (= E168)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
27% identity, 44% coverage: 5:223/501 of query aligns to 2:217/241 of 4u00A

query
sites
4u00A

I

I

L

I

K

R

L

I

S

R

G

N

I

L

E

H

K

K

S

W

F
|
F

P

G

G

P

V

L

K

H

A
x
V

L

L

D

K

N

G

A

I

C

H

L

L

N

E

V

V

Y

A

P

P

G

G

K

E

V

K

M

L

A

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

I

K

R

V

T

L

I

T

N

G

R

I

L

Y

E

H

D

M

F

D

Q

K

E

G

G

S

E

I

V

N

V

Y

V

Q

D

G

G

S

L

D

S

V

V

T

K

F

D

D

D

G

R

P

A

R

L

H

R

S

E

Q

I

E

R

V

R

G

E

I

V

S

G

I

M

I

V

H

F

Q

Q

E

Q

L

F

N

N

L

L

I

F

P

P

E

H

L

M

T

T

I

V

A

L

E

E

N

N

I

V

Y

T

L

L

G

A

R

P

E

M

T

R

T

V

N

R

A

-

F

-

G

-

I

-

K

R

W

W

T

P

Q

R

M

E

F

K

A

A

D

E

A

K

D

K

A

A

L

L

-

E

-

L

L

L

Q

E

R

R

L

V

N

G

V

I

K

L

H

D

S

Q

S

A

R

R

Q

K

L

Y

L

P

G

A

E

Q

L

L

S

S

L

G

G

G

E

Q

Q

Q

M

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

A

F

M

K

E

S

P

Q

K

V

I

I

M

V

L

M

F

D

D

E

E

P

P

T

T

D

S

A

A

L

L

T

D

E

P

S

E

E

M

T

V

K

G

S

E

L

V

F

L

K

D

V

V

I

M

N

R

E

D

L

L

R

A

N

Q

E

G

G

G

C

M

G

T

I

M

V

V

Y

V

I

V

S

T

H

H

R

E

L

M

K

G

E

F

I

A

F

R

E

E

I

V

C

A

D

D

D

R

I

V

T

V

V

F

L

M

R

D

D

G

G

G

K

Q

F

I

I

V

A

E

E

E

binding adenosine-5'-diphosphate: F12 (= F15), V17 (≠ A20), S37 (≠ N40), G38 (= G41), S39 (≠ A42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46)

3c4jA Abc protein artp in complex with atp-gamma-s
30% identity, 43% coverage: 11:223/501 of query aligns to 9:218/242 of 3c4jA

query
sites
3c4jA

I

L

E

K

K

K

S

S

F
|
F

P

G

G

S

V

L

K

E

A
x
V

L

L

D

K

N

G

A

I

C

N

L

V

N

H

V

I

Y

R

P

E

G

G

K

E

V

V

M

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

V

C

L

L

T

N

G

L

I

L

Y

E

H

D

M

F

D

D

K

E

G

G

S

E

I

I

-

I

-

D

-

G

-

I

N

N

Y

L

Q

K

G

A

S

K

D

D

V

T

T

N

F

L

D

N

G

K

P

V

R

R

H

-

S

-

Q

E

E

E

V

V

G

G

I

M

S

-

I

-

I

V

H

F

Q

Q

E

R

L

F

N

N

L

L

I

F

P

P

E

H

L

M

T

T

I

V

A

L

E

N

N

N

I

I

Y

T

L

L

G

A

R

P

E

M

T

K

T

V

N

R

A

-

F

-

G

-

I

-

K

K

W

W

T

P

Q

R

M

E

F

K

A

A

D

E

A

A

D

K

A

A

-

M

-

E

L

L

Q

D

R

K

L

V

N

G

V

L

K

K

H

D

S

K

S

A

R

H

Q

A

L

Y

L

P

G

D

E

S

L

L

S

S

L

G

G

G

E

Q

Q

A

Q

Q

M

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

A

F

M

K

E

S

P

Q

K

V

I

I

M

V

L

M

F

D

D

E

E

P

P

T

T

D

S

A

A

L

L

T

D

E

P

S

E

E

M

T

V

K

G

S

E

L

V

F

L

K

S

V

V

I

M

N

K

E

Q

L

L

R

A

N

N

E

E

G

G

C

M

G

T

I

M

V

V

Y

V

I

V

S

T

H

H

R

E

L

M

K

G

E

F

I

A

F

R

E

E

I

V

C

G

D

D

D

R

I

V

T

L

V

F

L

M

R

-

D

D

G

G

K

G

F

Y

I

I

A

I

E

E

binding phosphothiophosphoric acid-adenylate ester: F13 (= F15), V18 (≠ A20), S38 (≠ N40), G39 (= G41), S40 (≠ A42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

3c41J Abc protein artp in complex with amp-pnp/mg2+
30% identity, 43% coverage: 11:223/501 of query aligns to 9:218/242 of 3c41J

query
sites
3c41J

I

L

E

K

K

K

S

S

F
|
F

P

G

G

S

V

L

K

E

A
x
V

L

L

D

K

N

G

A

I

C

N

L

V

N

H

V

I

Y

R

P

E

G

G

K

E

V

V

M

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

V

C

L

L

T

N

G

L

I

L

Y

E

H

D

M

F

D

D

K

E

G

G

S

E

I

I

-

I

-

D

-

G

-

I

N

N

Y

L

Q

K

G

A

S

K

D

D

V

T

T

N

F

L

D

N

G

K

P

V

R

R

H

-

S

-

Q

E

E

E

V

V

G

G

I

M

S

-

I

-

I

V

H

F

Q

Q

E

R

L

F

N

N

L

L

I

F

P

P

E

H

L

M

T

T

I

V

A

L

E

N

N

N

I

I

Y

T

L

L

G

A

R

P

E

M

T

K

T

V

N

R

A

-

F

-

G

-

I

-

K

K

W

W

T

P

Q

R

M

E

F

K

A

A

D

E

A

A

D

K

A

A

-

M

-

E

L

L

Q

D

R

K

L

V

N

G

V

L

K

K

H

D

S

K

S

A

R

H

Q

A

L

Y

L

P

G

D

E

S

L

L

S

S

L

G

G

G

E

Q

Q

A

Q

Q

M

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

A

F

M

K

E

S

P

Q

K

V

I

I

M

V

L

M

F

D
|
D

E

E

P

P

T

T

D

S

A

A

L

L

T

D

E

P

S

E

E

M

T

V

K

G

S

E

L

V

F

L

K

S

V

V

I

M

N

K

E

Q

L

L

R

A

N

N

E

E

G

G

C

M

G

T

I

M

V

V

Y

V

I

V

S

T

H

H

R

E

L

M

K

G

E

F

I

A

F

R

E

E

I

V

C

G

D

D

D

R

I

V

T

L

V

F

L

M

R

-

D

D

G

G

K

G

F

Y

I

I

A

I

E

E

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F15), V18 (≠ A20), S38 (≠ N40), G39 (= G41), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)
binding magnesium ion: S43 (= S45), D163 (= D167)

2olkA Abc protein artp in complex with adp-beta-s
30% identity, 43% coverage: 11:223/501 of query aligns to 9:218/242 of 2olkA

query
sites
2olkA

I

L

E

K

K

K

S

S

F
|
F

P

G

G

S

V

L

K

E

A
x
V

L

L

D

K

N

G

A

I

C

N

L

V

N

H

V

I

Y

R

P

E

G

G

K

E

V

V

M

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

V

C

L

L

T

N

G

L

I

L

Y

E

H

D

M

F

D

D

K

E

G

G

S

E

I

I

-

I

-

D

-

G

-

I

N

N

Y

L

Q

K

G

A

S

K

D

D

V

T

T

N

F

L

D

N

G

K

P

V

R

R

H

-

S

-

Q

E

E

E

V

V

G

G

I

M

S

-

I

-

I

V

H

F

Q

Q

E

R

L

F

N

N

L

L

I

F

P

P

E

H

L

M

T

T

I

V

A

L

E

N

N

N

I

I

Y

T

L

L

G

A

R

P

E

M

T

K

T

V

N

R

A

-

F

-

G

-

I

-

K

K

W

W

T

P

Q

R

M

E

F

K

A

A

D

E

A

A

D

K

A

A

-

M

-

E

L

L

Q

D

R

K

L

V

N

G

V

L

K

K

H

D

S

K

S

A

R

H

Q

A

L

Y

L

P

G

D

E

S

L

L

S

S

L

G

G

G

E

Q

Q

A

Q

Q

M

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

A

F

M

K

E

S

P

Q

K

V

I

I

M

V

L

M

F

D

D

E

E

P

P

T

T

D

S

A

A

L

L

T

D

E

P

S

E

E

M

T

V

K

G

S

E

L

V

F

L

K

S

V

V

I

M

N

K

E

Q

L

L

R

A

N

N

E

E

G

G

C

M

G

T

I

M

V

V

Y

V

I

V

S

T

H

H

R

E

L

M

K

G

E

F

I

A

F

R

E

E

I

V

C

G

D

D

D

R

I

V

T

L

V

F

L

M

R

-

D

D

G

G

K

G

F

Y

I

I

A

I

E

E

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F15), V18 (≠ A20), S38 (≠ N40), G39 (= G41), S40 (≠ A42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

2oljA Abc protein artp in complex with adp/mg2+
30% identity, 43% coverage: 11:223/501 of query aligns to 9:218/242 of 2oljA

query
sites
2oljA

I

L

E

K

K

K

S

S

F
|
F

P

G

G

S

V

L

K

E

A
x
V

L

L

D

K

N

G

A

I

C

N

L

V

N

H

V

I

Y

R

P

E

G

G

K

E

V

V

M

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

V

C

L

L

T

N

G

L

I

L

Y

E

H

D

M

F

D

D

K

E

G

G

S

E

I

I

-

I

-

D

-

G

-

I

N

N

Y

L

Q

K

G

A

S

K

D

D

V

T

T

N

F

L

D

N

G

K

P

V

R

R

H

-

S

-

Q

E

E

E

V

V

G

G

I

M

S

-

I

-

I

V

H

F

Q

Q

E

R

L

F

N

N

L

L

I

F

P

P

E

H

L

M

T

T

I

V

A

L

E

N

N

N

I

I

Y

T

L

L

G

A

R

P

E

M

T

K

T

V

N

R

A

-

F

-

G

-

I

-

K

K

W

W

T

P

Q

R

M

E

F

K

A

A

D

E

A

A

D

K

A

A

-

M

-

E

L

L

Q

D

R

K

L

V

N

G

V

L

K

K

H

D

S

K

S

A

R

H

Q

A

L

Y

L

P

G

D

E

S

L

L

S

S

L

G

G

G

E

Q

Q

A

Q

Q

M

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

A

F

M

K

E

S

P

Q

K

V

I

I

M

V

L

M

F

D

D

E

E

P

P

T

T

D

S

A

A

L

L

T

D

E

P

S

E

E

M

T

V

K

G

S

E

L

V

F

L

K

S

V

V

I

M

N

K

E

Q

L

L

R

A

N

N

E

E

G

G

C

M

G

T

I

M

V

V

Y

V

I

V

S

T

H

H

R

E

L

M

K

G

E

F

I

A

F

R

E

E

I

V

C

G

D

D

D

R

I

V

T

L

V

F

L

M

R

-

D

D

G

G

K

G

F

Y

I

I

A

I

E

E

binding adenosine-5'-diphosphate: F13 (= F15), V18 (≠ A20), S38 (≠ N40), G39 (= G41), S40 (≠ A42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
26% identity, 47% coverage: 4:238/501 of query aligns to 1:232/235 of 6mhzA

query
sites
6mhzA

A

A

I

T

L

L

K

T

L

A

S

K

G

N

I

L

E

A

K

K

S

A

F
x
Y

P

K

G

G

V

R

K

R

A

V

L

V

D

E

N

D

A

V

C

S

L

L

N

T

V

V

Y

N

P

S

G

G

K

E

V

I

M

V

A

G

L

L

G

G

E

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

F

K

Y

V

M

L

V

T

V

G

G

I

I

Y

V

H

P

M

R

D

D

K

A

G

G

S

N

I

I

N

I

Y

I

Q

D

G

D

S

D

D

D

V

I

T

S

F

L

D

L

G

-

P

P

R

L

H

H

S

A

Q

R

-

A

E

R

V

R

G

G

I

I

S

G

I

Y

I

L

H

P

Q
|
Q

E

E

L

A

N

S

L

I

I

F

P

R

E

R

L

L

T

S

I

V

A

Y

E

D

N

N

I

L

Y

M

L

A

G

V

R

L

E

Q

T

I

T

R

N

D

A

-

F

-

G

-

G

D

I

L

K

S

W

A

T

E

Q

Q

M

R

F

E

A

D

D

R

A

A

D

N

A

E

L

L

L

M

Q

E

R

E

L

F

N

H

V

I

K

E

H

H

S

L

S

R

R

D

Q

S

L

M

L

G

G

Q

E
x
S

L

L

S
|
S

L
x
G

G
|
G

E

E

Q

R

M

R

V

V

E

E

I

I

A

A

K

R

A

A

L

L

S

A

F

A

K

N

S

P

Q

K

V

F

I

I

V

L

M

L

D

D

E
|
E

P

P

T

F

D

A

A
x
G

L

V

T

D

E

P

S

I

E

S

T

V

K

I

S

D

L

I

F

K

K

R

V

I

I

I

N

E

E

H

L

L

R

R

N

D

E

S

G

G

C

L

G

G

I

V

V

L

Y

I

I

T

S

D

H
|
H

R

N

L

V

K

R

E

E

I

T

F

L

E

A

I

V

C

C

D

E

D

R

I

A

T

Y

V

I

L

V

R

S

D

Q

G

G

K

H

F

L

I

I

A

A

E

H

I

G

A

T

V

P

T

T

D

E

I

I

D

L

E

Q

D

D

G

E

L

H

I

V

E

K

K

R

M

V

binding adp orthovanadate: Y12 (≠ F15), N37 (= N40), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46), Q84 (= Q87), S136 (≠ E142), S138 (= S144), G139 (≠ L145), G140 (= G146), E162 (= E168), G166 (≠ A172), H194 (= H200)

6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
26% identity, 47% coverage: 4:238/501 of query aligns to 1:232/233 of 6b8bA

query
sites
6b8bA

A

A

I

T

L

L

K

T

L

A

S

K

G

N

I

L

E

A

K

K

S

A

F
x
Y

P

K

G

G

V
x
R

K

R

A
x
V

L

V

D

E

N

D

A

V

C

S

L

L

N

T

V

V

Y

N

P

S

G

G

K

E

V

I

M

V

A

G

L

L

G

G

E

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

F

K

Y

V

M

L

V

T

V

G

G

I

I

Y

V

H

P

M

R

D

D

K

A

G

G

S

N

I

I

N

I

Y

I

Q

D

G

D

S

D

D

D

V

I

T

S

F

L

D

-

G

L

P

P

R

L

H

H

S

A

Q

R

-

A

E

R

V

R

G

G

I

I

S

G

I

Y

I

L

H

P

Q

Q

E

E

L

A

N

S

L

I

I
x
F

P
x
R

E
x
R

L

L

T

S

I

V

A

Y

E

D

N

N

I

L

Y

M

L

A

G

V

R

L

E

Q

T

I

T

R

N

D

A

-

F

-

G

-

G

D

I

L

K

S

W

A

T

E

Q

Q

M

R

F

E

A

D

D

R

A

A

D

N

A

E

L

L

L

M

Q

E

R

E

L

F

N

H

V

I

K

E

H

H

S

L

S

R

R

D

Q

S

L

M

L

G

G

Q

E

S

L

L

S

S

L

G

G

G

E

E

Q

R

M

R

V

V

E

E

I

I

A

A

K

R

A

A

L

L

S

A

F

A

K

N

S

P

Q

K

V

F

I

I

V

L

M

L

D

D

E

E

P

P

T

F

D

A

A

G

L

V

T

D

E

P

S

I

E

S

T

V

K

I

S

D

L

I

F

K

K

R

V

I

I

I

N

E

E

H

L

L

R

R

N

D

E

S

G

G

C

L

G

G

I

V

V

L

Y

I

I

T

S

D

H

H

R

N

L

V

K

R

E

E

I

T

F

L

E

A

I

V

C

C

D

E

D

R

I

A

T

Y

V

I

L

V

R

S

D

Q

G

G

K

H

F

L

I

I

A

A

E

H

I

G

A

T

V

P

T

T

D

E

I

I

D

L

E

Q

D

D

G

E

L

H

I

V

E

K

K

R

M

V

binding adenosine-5'-diphosphate: Y12 (≠ F15), R15 (≠ V18), V17 (≠ A20), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46)
binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (≠ I92), R90 (≠ P93), R91 (≠ E94)

6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
26% identity, 47% coverage: 4:238/501 of query aligns to 1:232/238 of 6s8nB

query
sites
6s8nB

A

A

I

T

L

L

K

T

L

A

S

K

G

N

I

L

E

A

K

K

S

A

F

Y

P

K

G

G

V

R

K

R

A

V

L

V

D

E

N

D

A

V

C

S

L

L

N

T

V

V

Y

N

P

S

G

G

K

E

V

I

M

V

A

G

L

L

G

G

E

P

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

T

M

F

K

Y

V

M

L

V

T

V

G

G

I

I

Y

V

H

P

M

R

D

D

K

A

G

G

S

N

I

I

N

I

Y

I

Q

D

G

D

S

D

D

D

V

I

T

S

F

L

D

-

G

L

P

P

R

L

H

H

S

A

Q

R

-

A

E

R

V

R

G

G

I

I

S

G

I

Y

I

L

H

P

Q

Q

E

E

L

A

N

S

L

I

I

F

P
x
R

E

R

L

L

T

S

I

V

A

Y

E

D

N

N

I

L

Y

M

L

A

G

V

R

L

E

Q

T

I

T

R

N

D

A

-

F

-

G

-

G

D

I

L

K

S

W

A

T

E

Q

Q

M

R

F

E

A

D

D

R

A

A

D

N

A

E

L

L

L

M

Q

E

R

E

L

F

N

H

V

I

K

E

H

H

S

L

S

R

R

D

Q

S

L
x
M

L
x
G

G
x
Q

E

S

L

L

S

S

L

G

G

G

E

E

Q

R

M

R

V

V

E

E

I

I

A

A

K

R

A

A

L

L

S

A

F

A

K

N

S

P

Q

K

V

F

I

I

V

L

M

L

D

D

E

E

P

P

T

F

D

A

A

G

L

V

T

D

E

P

S

I

E

S

T

V

K

I

S

D

L

I

F

K

K

R

V

I

I

I

N

E

E

H

L

L

R

R

N

D

E

S

G

G

C

L

G

G

I

V

V

L

Y

I

I

T

S

D

H

H

R

N

L

V

K

R

E

E

I

T

F

L

E

A

I

V

C

C

D

E

D

R

I

A

T

Y

V

I

L

V

R

S

D

Q

G

G

K

H

F

L

I

I

A

A

E

H

I

G

A

T

V

P

T

T

D

E

I

I

D

L

E

Q

D

D

G

E

L

H

I

V

E

K

K

R

M

V

binding 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione: R90 (≠ P93), M133 (≠ L139), G134 (≠ L140), Q135 (≠ G141)

6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
26% identity, 47% coverage: 4:238/501 of query aligns to 1:232/238 of 6s8gA

query
sites
6s8gA

A

A

I

T

L

L

K

T

L

A

S

K

G

N

I

L

E

A

K

K

S

A

F
x
Y

P

K

G

G

V
x
R

K

R

A

V

L

V

D

E

N

D

A

V

C

S

L

L

N

T

V

V

Y

N

P

S

G

G

K

E

V

I

M

V

A

G

L

L

G

G

E

P

N
|
N

G

G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

F

K

Y

V

M

L

V

T

V

G

G

I

I

Y

V

H

P

M

R

D

D

K

A

G

G

S

N

I

I

N

I

Y

I

Q

D

G

D

S

D

D

D

V

I

T

S

F

L

D

-

G

L

P

P

R

L

H

H

S

A

Q

R

-

A

E

R

V

R

G

G

I

I

S

G

I

Y

I

L

H

P

Q
|
Q

E

E

L

A

N

S

L

I

I

F

P

R

E

R

L

L

T

S

I

V

A

Y

E

D

N

N

I

L

Y

M

L

A

G

V

R

L

E

Q

T

I

T

R

N

D

A

-

F

-

G

-

G

D

I

L

K

S

W

A

T

E

Q

Q

M

R

F

E

A

D

D

R

A

A

D

N

A

E

L

L

L

M

Q

E

R

E

L

F

N

H

V

I

K

E

H

H

S

L

S

R

R

D

Q

S

L

M

L

G

G

Q

E
x
S

L

L

S
|
S

L

G

G

G

E
|
E

Q

R

M

R

V

V

E

E

I

I

A

A

K

R

A

A

L

L

S

A

F

A

K

N

S

P

Q

K

V

F

I

I

V

L

M

L

D

D

E

E

P

P

T

F

D

A

A

G

L

V

T

D

E

P

S

I

E

S

T

V

K

I

S

D

L

I

F

K

K

R

V

I

I

I

N

E

E

H

L

L

R

R

N

D

E

S

G

G

C

L

G

G

I

V

V

L

Y

I

I

T

S

D

H

H

R

N

L

V

K

R

E

E

I

T

F

L

E

A

I

V

C

C

D

E

D

R

I

A

T

Y

V

I

L

V

R

S

D

Q

G

G

K

H

F

L

I

I

A

A

E

H

I

G

A

T

V

P

T

T

D

E

I

I

D

L

E

Q

D

D

G

E

L

H

I

V

E

K

K

R

M

V

binding phosphoaminophosphonic acid-adenylate ester: Y12 (≠ F15), R15 (≠ V18), N37 (= N40), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46), Q84 (= Q87), S136 (≠ E142), S138 (= S144), E141 (= E147)

6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
26% identity, 47% coverage: 4:238/501 of query aligns to 1:232/234 of 6b89A

query
sites
6b89A

A

A

I

T

L

L

K

T

L

A

S

K

G

N

I

L

E

A

K

K

S

A

F
x
Y

P

K

G

G

V
x
R

K

R

A
x
V

L

V

D

E

N

D

A

V

C

S

L

L

N

T

V

V

Y

N

P

S

G

G

K

E

V

I

M

V

A

G

L

L

G

G

E

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

F

K

Y

V

M

L

V

T

V

G

G

I

I

Y

V

H

P

M

R

D

D

K

A

G

G

S

N

I

I

N

I

Y

I

Q

D

G

D

S

D

D

D

V

I

T

S

F

L

D

-

G

L

P

P

R
x
L

H
|
H

S

A

Q

R

-

A

E

R

V

R

G

G

I

I

S

G

I

Y

I

L

H
x
P

Q
|
Q

E

E

L
x
A

N
x
S

L

I

I
x
F

P
x
R

E
x
R

L
|
L

T

S

I

V

A

Y

E

D

N

N

I

L

Y

M

L

A

G
x
V

R

L

E

Q

T

I

T

R

N

D

A

-

F

-

G

-

G

D

I

L

K

S

W

A

T

E

Q

Q

M

R

F

E

A

D

D

R

A

A

D

N

A

E

L

L

L

M

Q

E

R

E

L

F

N

H

V

I

K

E

H

H

S

L

S

R

R

D

Q

S

L

M

L

G

G
x
Q

E

S

L

L

S

S

L

G

G

G

E

E

Q

R

M

R

V

V

E

E

I

I

A

A

K
x
R

A

A

L

L

S

A

F

A

K

N

S

P

Q

K

V

F

I

I

V

L

M

L

D

D

E

E

P

P

T

F

D

A

A

G

L

V

T

D

E

P

S

I

E

S

T

V

K

I

S

D

L

I

F

K

K

R

V

I

I

I

N

E

E

H

L

L

R

R

N

D

E

S

G

G

C

L

G

G

I

V

V

L

Y

I

I

T

S

D

H

H

R

N

L

V

K

R

E

E

I

T

F

L

E

A

I

V

C

C

D

E

D

R

I

A

T

Y

V

I

L

V

R

S

D

Q

G

G

K

H

F

L

I

I

A

A

E

H

I

G

A

T

V

P

T

T

D

E

I

I

D

L

E

Q

D

D

G

E

L

H

I

V

E

K

K

R

M

V

binding adenosine-5'-diphosphate: Y12 (≠ F15), R15 (≠ V18), V17 (≠ A20), N37 (= N40), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46)
binding magnesium ion: T42 (≠ S45), Q84 (= Q87)
binding novobiocin: L71 (≠ R75), H72 (= H76), P83 (≠ H86), A86 (≠ L89), S87 (≠ N90), F89 (≠ I92), R90 (≠ P93), R91 (≠ E94), L92 (= L95), V101 (≠ G104), Q135 (≠ G141), R149 (≠ K155)

4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
26% identity, 47% coverage: 4:238/501 of query aligns to 1:232/234 of 4p31A

query
sites
4p31A

A

A

I

T

L

L

K

T

L

A

S

K

G

N

I

L

E

A

K

K

S

A

F
x
Y

P

K

G

G

V
x
R

K

R

A
x
V

L

V

D

E

N

D

A

V

C

S

L

L

N

T

V

V

Y

N

P

S

G

G

K

E

V

I

M

V

A

G

L

L

G

G

E

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

F

K

Y

V

M

L

V

T

V

G

G

I

I

Y

V

H

P

M

R

D

D

K

A

G

G

S

N

I

I

N

I

Y

I

Q

D

G

D

S

D

D

D

V

I

T

S

F

L

D

-

G

L

P

P

R

L

H

H

S

A

Q

R

-

A

E

R

V

R

G

G

I

I

S

G

I

Y

I

L

H

P

Q
|
Q

E

E

L

A

N

S

L

I

I

F

P

R

E

R

L

L

T

S

I

V

A

Y

E

D

N

N

I

L

Y

M

L

A

G

V

R

L

E

Q

T

I

T

R

N

D

A

-

F

-

G

-

G

D

I

L

K

S

W

A

T

E

Q

Q

M

R

F

E

A

D

D

R

A

A

D

N

A

E

L

L

L

M

Q

E

R

E

L

F

N

H

V

I

K

E

H

H

S

L

S

R

R

D

Q

S

L

M

L

G

G

Q

E

S

L

L

S

S

L

G

G

G

E

E

Q

R

M

R

V

V

E

E

I

I

A

A

K

R

A

A

L

L

S

A

F

A

K

N

S

P

Q

K

V

F

I

I

V

L

M

L

D

D

E

E

P

P

T

F

D

A

A

G

L

V

T

D

E

P

S

I

E

S

T

V

K

I

S

D

L

I

F

K

K

R

V

I

I

I

N

E

E

H

L

L

R

R

N

D

E

S

G

G

C

L

G

G

I

V

V

L

Y

I

I

T

S

D

H

H

R

N

L

V

K

R

E

E

I

T

F

L

E

A

I

V

C

C

D

E

D

R

I

A

T

Y

V

I

L

V

R

S

D

Q

G

G

K

H

F

L

I

I

A

A

E

H

I

G

A

T

V

P

T

T

D

E

I

I

D

L

E

Q

D

D

G

E

L

H

I

V

E

K

K

R

M

V

binding adenosine-5'-diphosphate: Y12 (≠ F15), R15 (≠ V18), V17 (≠ A20), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46)
binding magnesium ion: T42 (≠ S45), Q84 (= Q87)

8k1pB Mycobacterial efflux pump, adp+vanadate bound state
31% identity, 44% coverage: 3:221/501 of query aligns to 1:208/213 of 8k1pB

query
sites
8k1pB

Q

Q

A

V

I

P

L

I

K

E

L

I

S

R

G

G

I

L

E

T

K

K

S

H

F
|
F

P

G

G

S

V

V

K

R

A

A

L

L

D

D

N

G

A

L

C

D

L

L

N

T

V

V

Y

R

P

E

G

G

K

E

V

V

M

H

A

G

L

F

L

L

G

G

E

P

N
|
N

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

T

M

L

K

R

V

I

L

L

T

L

G

G

I

L

Y

V

H

K

M

A

D

D

K

G

G

G

S

S

I

V

N

R

Y

L

Q

L

G

G

S

G

D

D

V

P

T

W

F

T

D

D

G

A

-

V

-

D

-

L

P

H

R

R

H

H

S

-

Q

-

E

-

V

-

G

-

I

I

S

A

I

Y

I

V

H

P

Q

G

E

D

L

V

N

T

L

L

I

W

P

P

E

S

L

L

T

T

I

G

A

G

E

E

N

T

I

I

Y

D

L

L

G

-

R

-

E

-

T

L

T

A

N

R

A

M

F

R

G

G

I

I

K

D

W

-

T

-

Q

-

M

-

F

N

A

A

D

R

A

R

D

A

A

E

L

L

L

I

Q

E

R

R

L

F

N

G

V

L

K

D

H

P

S

T

S

K

R

K

Q

A

L

R

L

-

G

-

E
x
T

L

Y

S
|
S

L

K

G

G

E

N

Q

R

Q

Q

M

K

V

V

E

S

I

L

A

I

K

S

A

A

L

L

S

S

F

S

K

H

S

A

Q

T

V

L

I

L

V

L

M

L

D

D

E

E

P

P

T

S

D

S

A

G

L

L

T

D

E

P

S

L

E

M

T

E

K

N

S

V

L

F

F

Q

K

Q

V

C

I

I

N

G

E

E

L

A

R

R

N

Q

E

R

G

G

C

V

G

T

I

V

V

L

Y

L

I

S

S

S

H

H

R

I

L

L

K

A

E

E

I

T

F

E

E

A

I

L

C

C

D

E

D

K

I

V

T

T

V

I

L

I

R

R

D

A

G

G

K

K

F

T

I

V

binding adenosine-5'-diphosphate: F13 (= F15), N38 (= N40), G39 (= G41), A40 (= A42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)
binding adenosine-5'-triphosphate: T129 (≠ E142), S131 (= S144)

8wdbD Cryo-em structure of the atp-bound dppabcd complex
25% identity, 94% coverage: 5:473/501 of query aligns to 3:487/513 of 8wdbD

query
sites
8wdbD

I

L

L

L

K

S

L

V

S

E

G

G

I

L

E

E

K

V

S

T

F
|
F

-

G

-

T

-

D

-

A

P

P

G

A

V

V

K

C

A

G

L

V

D

D

N

-

A

-

C

-

L

L

N

A

V

V

Y

R

P

S

G

G

K

Q

V

T

M

V

A

A

L

V

G

G

E

E

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

T

M

A

K

A

V

A

L

I

T

L

G

G

I

L

Y

L

-

P

-

A

-

G

H

R

M

I

D

T

K

A

G

G

S

R

I

V

N

V

Y

F

Q

D

G

G

S

R

D

D