SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_017223156.1 NCBI__GCF_000276805.1:WP_017223156.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
70% identity, 100% coverage: 1:646/648 of query aligns to 1:646/652 of P27550

query
sites
P27550

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K609 (= K609) modified: N6-acetyllysine; by autocatalysis

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
70% identity, 100% coverage: 1:646/648 of query aligns to 1:646/652 of Q8ZKF6

query
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Q8ZKF6

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R194 (= R194) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T311) binding CoA
N335 (= N335) binding CoA
A357 (= A357) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D517) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S523) binding CoA
G524 (= G524) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R526) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (= R584) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K609) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
70% identity, 99% coverage: 7:646/648 of query aligns to 3:639/640 of 5jrhA

query
sites
5jrhA

Y

H

P

A

V

I

P

P

A

A

E

N

F

I

A

A

Q

D

N

R

S

C

L

L

L

I

N

N

D

P

S

E

A

Q

Y

Y

Q

E

A

T

M

K

Y

Y

K

K

H

Q

S

S

I

I

T

N

D

D

P

P

E

D

A

T

F

F

W

W

G

G

E

E

Q

Q

G

G

H

K

I

I

V

L

D

D

W

W

I

I

K

T

P

P

Y

Y

S

Q

Q

K

V

V

K

K

S

N

T

T

S

S

F

F

Q

A

P

P

G

G

S

N

V

V

D

S

I

I

K

K

W

W

F

Y

Q

E

D

D

G

G

Q

T

L

L

N

N

V

L

S

A

A

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

K

Q

D

E

N

N

A

G

N

D

K

R

T

T

A

A

I

I

W
|
W

E

E

G

G

D

D

N

D

P

T

E

S

D

Q

S

S

E

K

K

H

L

I

T

S

Y

Y

Q

R

D

E

V

L

Y

H

E

R

K

D

V

V

C

C

Q

R

F

F

S

A

N

N

A

T

L

L

K

L

A

D

Q

L

G

G

I

I

K

K

G

G

D

D

V

V

C

A

L

I

Y

Y

M

M

P

P

M

M

V

V

P

P

E
|
E

A

A

I

V

A

A

M

M

L

L

A

A

C

C

T

A

R

R

I

I

G

G

A

A

V

V

H

H

T

S

I

V

V

I

F
|
F

A
x
G

G
|
G

F

F

S

S

A

P

E

E

A

A

L

V

A

A

D
x
G

R

R

I

I

D

I

N

D

C

S

G

S

A

S

K

R

L

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R
|
R

A

A

G

G

R

R

I

S

T

I

R

P

L

L

K

K

D

K

C

N

V

V

D

D

T

D

A

A

L

L

A

K

K

N

P

P

Q

N

V

V

T

T

T

S

V

V

N

E

N

H

V

V

I

I

V

V

F

L

A

K

R

R

T

T

K

G

A

S

D

D

I

I

A

D

W

W

H

Q

E

E

G

G

R

R

D

D

L

L

W

W

H

R

D

D

V

L

V

I

K

E

D

K

Q

A

P

S

T

P

E

E

C

H

V

Q

P

P

E

E

A

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

T

A

M

T

T

T

F

F

K

K

Y

Y

V

V

F

F

D

D

H

Y

Q

H

D

P

T

G

D

D

V

I

F

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

I

V

T

T

G

G

H

H

S

S

Y

Y

L

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

A

G

A

A

T

T

V

L

L

M

F

F

E

E

G

G

V

V

P

P

N

N

Y

W

P

P

D

T

T

P

A

A

R

R

M

M

S

C

Q

Q

V

V

D

D

K

K

H

H

Q

Q

V

V

T

N

T

I

L

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

A

A

K

E

G

G

D

D

D

K

A

A

I

I

K

E

G

G

T

T

K

D

R

R

T

S

S

S

L

L

R

R

L

I

M

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

Q

E

W

W

Y

Y

Y

W

N

K

T

K

I

I

G

G

N

K

A

E

S

K

T

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

M

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

I

V

S

Q

P

P

A

A

L

L

V

V

D

D

N

N

E

E

G

G

N

H

I

P

L

Q

E

E

G

G

A

A

N

T

E

E

G

G

N

N

L

L

V

V

I

I

L

T

D

D

S

S

W

W

P

P

G

G

Q

Q

M

A

R

R

G

T

I

L

Y

F

G

G

D

D

E

H

D

E

R

R

F

F

E

E

E

Q

A

T

Y

Y

F

F

S

S

T

T

F

F

P

K

N

N

M

M

Y

Y

C

F

T

S

G

G

D
|
D

G

G

A

A

K

R

R

R

D

D

L

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R

R

V

V

D

D

V

V

L

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

L

L

V
|
V

S

A

H
|
H

S

P

K

K

I
|
I

A

A

E

E

A

A

V

V

G

G

V

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

T

T

L

L

N

N

A

H

G

G

E

E

P

P

D

S

A

P

A

E

L

L

L

Y

A

A

E

E

V

V

K

R

Q

N

W

W

V

V

R
|
R

S

K

E

E

I

I

G

G

A
x
P

I

L

A

A

T

T

P

P

D

D

T

V

L

L

H

H

W

W

T

T

Q

D

A

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

V

I

A

A

T

A

N

G

D

D

T

-

D

-

S

-

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

S

G

V

V

D

E

T

K

L

L

I

L

A

E

E

E

K

K

Q

Q

active site: T260 (= T264), T412 (= T416), E413 (= E417), N517 (= N521), R522 (= R526), K605 (= K609)
binding (r,r)-2,3-butanediol: W93 (= W97), E140 (= E144), G169 (≠ D173), K266 (= K270), P267 (= P271)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G387), E384 (= E388), P385 (= P389), T408 (= T412), W409 (= W413), W410 (= W414), Q411 (= Q415), T412 (= T416), D496 (= D500), I508 (= I512), N517 (= N521), R522 (= R526)
binding coenzyme a: F159 (= F163), G160 (≠ A164), G161 (= G165), R187 (= R191), S519 (= S523), R580 (= R584), P585 (≠ A589)
binding magnesium ion: V533 (= V537), H535 (= H539), I538 (= I542)

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
69% identity, 99% coverage: 7:646/648 of query aligns to 3:640/641 of 2p20A

query
sites
2p20A

Y

H

P

A

V

I

P

P

A

A

E

N

F

I

A

A

Q

D

N

R

S

C

L

L

L

I

N

N

D

P

S

E

A

Q

Y

Y

Q

E

A

T

M

K

Y

Y

K

K

H

Q

S

S

I

I

T

N

D

D

P

P

E

D

A

T

F

F

W

W

G

G

E

E

Q

Q

G

G

H

K

I

I

V

L

D

D

W

W

I

I

K

T

P

P

Y

Y

S

Q

Q

K

V

V

K

K

S

N

T

T

S

S

F

F

Q

A

P

P

G

G

S

N

V

V

D

S

I

I

K

K

W

W

F

Y

Q

E

D

D

G

G

Q

T

L

L

N

N

V

L

S

A

A

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

K

Q

D

E

N

N

A

G

N

D

K

R

T

T

A

A

I

I

W

W

E

E

G

G

D

D

N

D

P

T

E

S

D

Q

S

S

E

K

K

H

L

I

T

S

Y

Y

Q

R

D

E

V

L

Y

H

E

R

K

D

V

V

C

C

Q

R

F

F

S

A

N

N

A

T

L

L

K

L

A

D

Q

L

G

G

I

I

K

K

G

G

D

D

V

V

C

A

L

I

Y

Y

M

M

P

P

M

M

V

V

P

P

E

E

A

A

I

V

A

A

M

M

L

L

A

A

C

C

T

A

R

R

I

I

G

G

A

A

V

V

H

H

T

S

I

V

V

I

F

F

A

G

G

G

F

F

S

S

A

P

E

E

A

A

L

V

A

A

D

G

R

R

I

I

D

I

N

D

C

S

G

S

A

S

K

R

L

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R

R

A

A

G

G

R

R

I

S

T

I

R

P

L

L

K

K

D

K

C

N

V

V

D

D

T

D

A

A

L

L

A

K

K

N

P

P

Q

N

V

V

T

T

T

S

V

V

N

E

N

H

V

V

I

I

V

V

F

L

A

K

R

R

T

T

K

G

A

S

D

D

I

I

A

D

W

W

H

Q

E

E

G

G

R

R

D

D

L

L

W

W

H

R

D

D

V

L

V

I

K

E

D

K

Q

A

P

S

T

P

E

E

C

H

V

Q

P

P

E

E

A

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

T

A

M

T

T

T

F

F

K

K

Y

Y

V

V

F

F

D

D

H

Y

Q

H

D

P

T

G

D

D

V

I

F

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

I

V

T

T

G

G

H

H

S

S

Y

Y

L

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

A

G

A

A

T

T

V

L

L

M

F

F

E

E

G

G

V

V

P

P

N

N

Y

W

P

P

D

T

T

P

A

A

R

R

M

M

S

C

Q

Q

V

V

D

D

K

K

H

H

Q

Q

V

V

T

N

T

I

L

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

A

A

K

E

G

G

D

D

D

K

A

A

I

I

K

E

G

G

T

T

K

D

R

R

T

S

S

S

L

L

R

R

L

I

M

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

Q

E

W

W

Y

Y

Y

W

N

K

T

K

I

I

G

G

N

K

A

E

S

K

T

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

M

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

I

V

S

Q

P

P

A

A

L

L

V

V

D

D

N

N

E

E

G

G

N

H

I

P

L

Q

E

E

G

G

A

A

N

T

E

E

G

G

N

N

L

L

V

V

I

I

L

T

D

D

S

S

W

W

P

P

G

G

Q

Q

M

A

R

R

G

T

I

L

Y

F

G

G

D

D

E

H

D

E

R

R

F

F

E

E

E

Q

A

T

Y

Y

F

F

S

S

T

T

F

F

P

K

N

N

M

M

Y

Y

C

F

T

S

G

G

D
|
D

G

G

A

A

K

R

R

R

D

D

L

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

V

V

S

S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

S

P

K

K

I

I

A

A

E

E

A

A

V

V

G

G

V

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

T

T

L

L

N

N

A

H

G

G

E

E

P

P

D

S

A

P

A

E

L

L

L

Y

A

A

E

E

V

V

K

R

Q

N

W

W

V

V

R

A

S

K

E

E

I

I

G

G

A

P

I

L

A

A

T

T

P

P

D

D

T

V

L

L

H

H

W

W

T

T

Q

D

A

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

V

I

A

A

T

A

N

G

D

D

T

T

D

S

S

N

L

-

G

-

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

S

G

V

V

D

E

T

K

L

L

I

L

A

E

E

E

K

K

Q

Q

active site: T260 (= T264), T412 (= T416), E413 (= E417), N517 (= N521), R522 (= R526), K605 (= K609)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G387), E384 (= E388), P385 (= P389), T408 (= T412), W409 (= W413), W410 (= W414), Q411 (= Q415), T412 (= T416), D496 (= D500), I508 (= I512), R511 (= R515), R522 (= R526)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
69% identity, 99% coverage: 7:646/648 of query aligns to 2:636/637 of 2p2fA

query
sites
2p2fA

Y

H

P

A

V

I

P

P

A

A

E

N

F

I

A

A

Q

D

N

R

S

C

L

L

L

I

N

N

D

P

S

E

A

Q

Y

Y

Q

E

A

T

M

K

Y

Y

K

K

H

Q

S

S

I

I

T

N

D

D

P

P

E

D

A

T

F

F

W

W

G

G

E

E

Q

Q

G

G

H

K

I

I

V

L

D

D

W

W

I

I

K

T

P

P

Y

Y

S

Q

Q

K

V

V

K

K

S

N

T

T

S

S

F

F

Q

A

P

P

G

G

S

N

V

V

D

S

I

I

K

K

W

W

F

Y

Q

E

D

D

G

G

Q

T

L

L

N

N

V

L

S

A

A

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

K

Q

D

E

N

N

A

G

N

D

K

R

T

T

A

A

I

I

W

W

E

E

G

G

D

D

N

D

P

T

E

S

D

Q

S

S

E

K

K

H

L

I

T

S

Y

Y

Q

R

D

E

V

L

Y

H

E

R

K

D

V

V

C

C

Q

R

F

F

S

A

N

N

A

T

L

L

K

L

A

D

Q

L

G

G

I

I

K

K

G

G

D

D

V

V

C

A

L

I

Y

Y

M

M

P

P

M

M

V

V

P

P

E

E

A

A

I

V

A

A

M

M

L

L

A

A

C

C

T

A

R

R

I

I

G

G

A

A

V

V

H

H

T

S

I

V

V

I

F
|
F

A

G

G

G

F

F

S

S

A

P

E

E

A

A

L

V

A

A

D

G

R

R

I

I

D

I

N

D

C

S

G

S

A

S

K

R

L

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R
|
R

A

A

G

G

R

R

I

S

T

I

R

P

L

L

K

K

D

K

C

N

V

V

D

D

T

D

A

A

L

L

A

K

K

N

P

P

Q

N

V

V

T

T

T

S

V

V

N

E

N

H

V

V

I

I

V

V

F

L

A

K

R

R

T

T

K

G

A

S

D

D

I

I

A

D

W

W

H

Q

E

E

G

G

R

R

D

D

L

L

W

W

H

R

D

D

V

L

V

I

K

E

D

K

Q

A

P

S

T

P

E

E

C

H

V

Q

P

P

E

E

A

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

T

A

M

T

T

T

F

F

K

K

Y

Y

V

V

F

F

D

D

H

Y

Q

H

D

P

T

G

D

D

V

I

F

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W
|
W

I

V

T
|
T

G

G

H

H

S

S

Y

Y

L

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

A

G

A

A

T

T

V

L

L

M

F

F

E

E

G

G

V

V

P
|
P

N

N

Y

W

P

P

D

T

T

P

A

A

R

R

M

M

S

C

Q

Q

V

V

D

D

K

K

H

H

Q

Q

V

V

T

N

T

I

L

L

Y

Y

T

T

A
|
A

P

P

T

T

A
|
A

I

I

R

R

A

A

L

L

M

M

A

A

K

E

G

G

D

D

D

K

A

A

I

I

K

E

G

G

T

T

K

D

R

R

T

S

S

S

L

L

R

R

L

I

M

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

Q

E

W

W

Y

Y

Y

W

N

K

T

K

I

I

G

G

N

K

A

E

S

K

T

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

M

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

I

V

S

Q

P

P

A

A

L

L

V

V

D

D

N

N

E

E

G

G

N

H

I

P

L

Q

E

E

G

G

A

A

N

T

E

E

G

G

N

N

L

L

V

V

I

I

L

T

D

D

S

S

W

W

P

P

G

G

Q

Q

M

A

R

R

G

T

I

L

Y

F

G

G

D

D

E

H

D

E

R

R

F

F

E

E

E

Q

A

T

Y

Y

F

F

S

S

T

T

F

F

P

K

N

N

M

M

Y

Y

C

F

T

S

G

G

D
|
D

G

G

A

A

K

R

R

R

D

D

L

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

S

P

K

K

I

I

A

A

E

E

A

A

V

V

G

G

V

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

T

T

L

L

N

N

A

H

G

G

E

E

P

P

D

S

A

P

A

E

L

L

L

Y

A

A

E

E

V

V

K

R

Q

N

W

W

V

V

R
|
R

S

K

E

E

I

I

G

G

A
x
P

I

L

A

A

T

T

P

P

D

D

T

V

L

L

H

H

W

W

T

T

Q

D

A

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

V

I

A

A

T

A

N

G

D

D

T

T

D

-

S

-

L

-

G

-

D

-

T

S

S

S

T

T

L

L

A

A

D

D

P

P

S

G

V

V

D

E

T

K

L

L

I

L

A

E

E

E

K

K

Q

Q

active site: T259 (= T264), T411 (= T416), E412 (= E417), N516 (= N521), R521 (= R526), K604 (= K609)
binding adenosine monophosphate: G382 (= G387), E383 (= E388), P384 (= P389), T407 (= T412), W408 (= W413), W409 (= W414), Q410 (= Q415), T411 (= T416), D495 (= D500), I507 (= I512), R510 (= R515), N516 (= N521), R521 (= R526)
binding coenzyme a: F158 (= F163), R186 (= R191), W304 (= W309), T306 (= T311), P329 (= P334), A352 (= A357), A355 (= A360), S518 (= S523), R579 (= R584), P584 (≠ A589)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
69% identity, 99% coverage: 7:646/648 of query aligns to 3:633/634 of 1pg3A

query
sites
1pg3A

Y

H

P

A

V

I

P

P

A

A

E

N

F

I

A

A

Q

D

N

R

S

C

L

L

L

I

N

N

D

P

S

E

A

Q

Y

Y

Q

E

A

T

M

K

Y

Y

K

K

H

Q

S

S

I

I

T

N

D

D

P

P

E

D

A

T

F

F

W

W

G

G

E

E

Q

Q

G

G

H

K

I

I

V

L

D

D

W

W

I

I

K

T

P

P

Y

Y

S

Q

Q

K

V

V

K

K

S

N

T

T

S

S

F

F

Q

A

P

P

G

G

S

N

V

V

D

S

I

I

K

K

W

W

F

Y

Q

E

D

D

G

G

Q

T

L

L

N

N

V

L

S

A

A

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

K

Q

D

E

N

N

A

G

N

D

K

R

T

T

A

A

I

I

W

W

E

E

G

G

D

D

N

D

P

T

E

S

D

Q

S

S

E

K

K

H

L

I

T

S

Y

Y

Q

R

D

E

V

L

Y

H

E

R

K

D

V

V

C

C

Q

R

F

F

S

A

N

N

A

T

L

L

K

L

A

D

Q

L

G

G

I

I

K

K

G

G

D

D

V

V

C

A

L

I

Y

Y

M

M

P

P

M

M

V

V

P

P

E

E

A

A

I

V

A

A

M

M

L

L

A

A

C

C

T

A

R

R

I

I

G

G

A

A

V

V

H

H

T

S

I

V

V

I

F
|
F

A
x
G

G

G

F

F

S

S

A

P

E

E

A

A

L

V

A

A

D

G

R

C

I

I

D

I

N

D

C

S

G

S

A

S

K

R

L

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R
|
R

A

A

G

G

R
|
R

I

S

T

I

R

P

L

L

K

K

D

K

C

N

V

V

D

D

T

D

A

A

L

L

A

K

K

N

P

P

Q

N

V

V

T

T

T

S

V

V

N

E

N

H

V

V

I

I

V

V

F

L

A

K

R

R

T

T

K

G

A

S

D

D

I

I

A

D

W

W

H

Q

E

E

G

G

R

R

D

D

L

L

W

W

H

R

D

D

V

L

V

I

K

E

D

K

Q

A

P

S

T

P

E

E

C

H

V

Q

P

P

E

E

A

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

T

A

M

T

T

T

F

F

K

K

Y

Y

V

V

F

F

D

D

H

Y

Q

H

D

P

T

G

D

D

V

I

F

Y

W

W

C

C

T

T

A
|
A

D

D

V

V

G

G

W

W

I

V

T
|
T

G

G

H

H

S

S

Y

Y

L

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

A

G

A

A

T

T

V

L

L

M

F

F

E

E

G

G

V

V

P
|
P

N

N

Y

W

P

P

D

T

T

P

A

A

R

R

M

M

S

C

Q

Q

V

V

D

D

K

K

H

H

Q

Q

V

V

T

N

T

I

L

L

Y

Y

T

T

A

A

P

P

T

T

A
|
A

I

I

R

R

A

A

L

L

M

M

A

A

K

E

G

G

D

D

D

K

A

A

I

I

K

E

G

G

T

T

K

D

R

R

T

S

S

S

L

L

R

R

L

I

M

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

Q

E

W

W

Y

Y

Y

W

N

K

T

K

I

I

G

G

N

K

A

E

S

K

T

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

M

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

I

V

S

Q

P

P

A

A

L

L

V

V

D

D

N

N

E

E

G

G

N

H

I

P

L

Q

E

E

G

G

A

A

N

T

E

E

G

G

N

N

L

L

V

V

I

I

L

T

D

D

S

S

W

W

P

P

G

G

Q

Q

M

A

R

R

G

T

I

L

Y

F

G

G

D

D

E

H

D

E

R

R

F

F

E

E

E

Q

A

T

Y

Y

F

F

S

S

T

T

F

F

P

K

N

N

M

M

Y

Y

C

F

T

S

G

G

D
|
D

G

G

A

A

K

R

R

R

D

D

L

E

D

D

G

G

Y

Y

W

W

I

I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

L

L

V
|
V

S

A

H
|
H

S

P

K

K

I
|
I

A

A

E

E

A

A

V

V

G

G

V

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

T

T

L

L

N

N

A

H

G

G

E

E

P

P

D

S

A

P

A

E

L

L

L

Y

A

A

E

E

V

V

K

R

Q

N

W

W

V

V

R
|
R

S

K

E

E

I

I

G

G

A
x
P

I

L

A

A

T

T

P

P

D

D

T

V

L

L

H

H

W

W

T

T

Q

D

A

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

V

I

A

A

T

A

N

G

D

D

T

-

D

-

S

-

L

-

G

-

D

-

T

-

S

-

T

-

L

L

A

A

D

D

P

P

S

G

V

V

D

E

T

K

L

L

I

L

A

E

E

E

K

K

Q

Q

active site: T260 (= T264), T412 (= T416), E413 (= E417), N517 (= N521), R522 (= R526), K605 (= K609)
binding coenzyme a: F159 (= F163), G160 (≠ A164), R187 (= R191), R190 (= R194), A301 (= A305), T307 (= T311), P330 (= P334), A356 (= A360), S519 (= S523), R580 (= R584), P585 (≠ A589)
binding magnesium ion: V533 (= V537), H535 (= H539), I538 (= I542)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G387), E384 (= E388), P385 (= P389), T408 (= T412), W409 (= W413), W410 (= W414), Q411 (= Q415), T412 (= T416), D496 (= D500), R511 (= R515), R522 (= R526)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
62% identity, 99% coverage: 5:646/648 of query aligns to 4:643/648 of Q89WV5

query
sites
Q89WV5

K

K

L

I

Y

Y

P

D

V

V

P

P

A

A

E

E

F

W

A

A

Q

K

N

R

S

A

L

W

L

V

N

D

D

Q

S

A

A

K

Y

Y

Q

K

A

E

M

M

Y

Y

K

A

H

R

S

S

I

I

T

S

D

D

P

P

E

N

A

G

F

F

W

W

G

A

E

E

Q

Q

G

A

H

K

I

R

V

I

D

D

W

W

I

M

K

K

P

A

Y

P

S

T

Q

K

V

I

K

E

S

N

T

V

S

S

F

F

Q

A

P

P

G

G

S

N

V

V

D

S

I

I

K

K

W

W

F

F

Q

E

D

D

G

G

Q

V

L

L

N

N

V

V

S

A

A

H

N

N

C

C

L

I

D

D

R

R

H

H

L

L

K

H

D

K

N

R

A

A

N

N

K

Q

T

T

A

A

I

I

W

W

E

E

G

G

D

D

N

D

P

P

E

S

D

Q

S

S

E

R

K

H

L

I

T

T

Y

Y

Q

K

D

E

V

L

Y

H

E

D

K

E

V

V

C

C

Q

R

F

M

S

A

N

N

A

I

L

L

K

R

A

T

Q

R

G

N

I

V

K

K

G

G

D

D

V

R

V

V

C

T

L

I

Y

Y

M

L

P

P

M

M

V

I

P

P

E

E

A

A

I

Y

A

A

M

M

L

L

A

A

C

C

T

A

R

R

I

I

G

G

A

A

V

I

H

H

T

S

I

V

V

V

F

F

A

A

G

G

F

F

S

S

A

P

E

D

A

S

L

L

A

A

D

Q

R

R

I

I

D

N

N

D

C

C

G

Q

A

S

K

K

L

V

V

I

I

I

T

T

A

A

D

D

E

E

G

G

V

L

R

R

A

G

G

G

R

K

I

K

T

V

R

P

L

L

K

K

D

A

C

N

V

V

D

D

T

A

A

A

L

L

A

A

K

K

P

-

Q

-

V

A

T

D

T

G

V

V

N

D

N

W

V

V

I

V

V

V

F

V

A

K

R

R

T

T

K

G

A

G

D

K

I

I

A

D

W

M

H

N

E

P

G

T

R

R

D

D

L

L

W

W

W

Y

H

H

D

E

V

A

K

A

D

M

Q

V

P

T

T

T

E

E

C

C

V

P

P

V

E

E

A

H

M

M

N

H

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T

T

S

S

G
|
G

S

S

T

T

G
|
G

K

Q

P

P

K
|
K

G

G

V

V

L

L

H

H

T

T

T

S

G

A

G

G

Y

Y

L

L

V

V

Y

Y

A

A

T

A

M

M

T

T

F

H

K

Q

Y

Y

V

V

F

F

D

D

H

Y

Q

H

D

D

T

G

D

D

V

I

F

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

I

V

T

T

G

G

H

H

S

S

Y

Y

L

I

V

L

Y

Y

G

G

P

P

L

L

A

A

N

N

A

G

A

A

T

T

V

L

L

M

F

F

E

E

G

G

V

V

P

P

N

N

Y

Y

P

P

D

D

T

N

A

S

R

R

M

F

S

W

Q

N

V

V

V

I

D

D

K

K

H

H

Q

K

V

V

T

N

T

T

L

F

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

A

Q

K

G

G

G

D

D

D

E

A

P

I

V

K

K

G

K

T

T

K

S

R

R

T

A

S

S

L

L

R

R

L

L

M

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

W

W

Q

E

W

W

Y

Y

Y

H

N

R

T

V

I

V

G

G

N

E

A

D

S

R

T

C

P

P

I

I

V

V

D

D

T

T

W

W

Q

Q

T

T

E
|
E

T

T

G

G

I

I

L

L

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

T

M

K

L

L

K

K

P

P

G

G

S

S

A

A

T

T

R

Q

P

P

F

F

G

G

I

V

S

V

P

P

A

E

L

I

V

V

D

D

N

A

E

D

G

G

N

K

I

V

L

L

E

E

G

G

A

E

N

T

E

T

G

G

N

N

L

L

V

C

I

L

L

T

D

R

S

A

W

W

P

P

G

G

Q

M

M

M

R

R

G

T

I

V

Y

Y

G

G

D

D

E

H

D

A

R

R

F

F

E

E

E

Q

A

T

Y

Y

F

F

S

S

T

T

F

Y

P

K

N

G

M

K

Y

Y

C

F

T

T

G

G

D

D

G

G

A

C

K

R

R

R

D

D

L

A

D

D

G

G

Y

Y

W

W

I

I

T

T

G

G

R

R

V

V

D

D

V

V

L

I

N

N

V

V

S

S

G

G

H

H

R

R

M

M

G

G

T

T

A

A

E

E

I

V

E

E

S

S

A

A

L

L

V

V

S

A

H

H

S

E

K

K

I

V

A

S

E

E

A

A

V

V

G

G

V

F

P

P

H

H

D

D

I

I

K

K

G

G

Q

Q

G

G

I

I

Y

Y

A

A

Y

Y

V

V

T

T

L

L

N

M

A

A

G

G

E

V

E

Q

P

P

D

T

A

E

A

D

L

L

L

R

A

K

E

E

V

L

K

V

Q

T

W

W

V

V

R

R

S

K

E

E

I

I

G

G

A

P

I

I

A

A

T

S

P

P

D

D

T

Q

L

I

H

Q

W

F

T

A

Q

P

A

G

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K

K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

V

I

A

A

T

E

N

D

D

E

T

P

D

G

S

S

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

S

A

V

V

D

D

T

D

L

L

I

V

A

K

E

N

K

R

Q

Q

G263 (= G266) mutation to I: Loss of activity.
G266 (= G269) mutation to I: Great decrease in activity.
K269 (= K272) mutation to G: Great decrease in activity.
E414 (= E417) mutation to Q: Great decrease in activity.

8sf3A Crystal structure of acetyl-coenzyme a synthetase from leishmania infantum (amp, acetate and coa bound)
53% identity, 96% coverage: 27:647/648 of query aligns to 28:659/662 of 8sf3A

query
sites
8sf3A

M

I

Y

Y

K

E

H

Y

S

S

I

I

T

E

D

H

P

N

E

D

A

A

F

F

W

W

G

A

E

E

Q

I

G

A

H

R