SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_017257106.1 NCBI__GCF_000302595.1:WP_017257106.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
59% identity, 98% coverage: 10:634/635 of query aligns to 22:642/648 of Q89WV5

query
sites
Q89WV5

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G263 (= G254) mutation to I: Loss of activity.
G266 (= G257) mutation to I: Great decrease in activity.
K269 (= K260) mutation to G: Great decrease in activity.
E414 (= E405) mutation to Q: Great decrease in activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
55% identity, 98% coverage: 10:634/635 of query aligns to 23:645/652 of P27550

query
sites
P27550

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K609 (= K598) modified: N6-acetyllysine; by autocatalysis

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
55% identity, 99% coverage: 9:634/635 of query aligns to 22:645/652 of Q8ZKF6

query
sites
Q8ZKF6

D

E

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V

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|
T

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|
A

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L

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V

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P

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N

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W

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G

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M

M

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F

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G

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A

L

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D

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D

G

G

Y

Y

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W

I

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T

G

G

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R

V

V

D
|
D

D

D

V

V

I

L

N

N

V

V

S
|
S

G
|
G

H

H

R
|
R

I

L

G

G

T

T

A

A

E

E

V

I

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N

S

A

A

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L

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V

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L

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V

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x
R

R

K

I

E

I

I

G

G

A

P

I

L

A

A

R

T

P

P

D

D

K

V

I

L

Q

H

F

W

V

T

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S

L

L

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P

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K

T

T

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R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

G

G

D

D

M

T

K

S

N

N

V

L

G

G

D

D

V

T

S

S

T

T

L

L

L

A

D

D

P

P

A

G

V

V

E

E

E

K

I

L

K

E

G

E

K

K

R194 (≠ K180) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T299) binding CoA
N335 (≠ T323) binding CoA
A357 (= A345) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D506) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S512) binding CoA
G524 (= G513) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R515) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (≠ S573) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K598) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
54% identity, 99% coverage: 9:634/635 of query aligns to 18:639/641 of 2p20A

query
sites
2p20A

D

E

E

Q

Y

Y

K

E

E

T

A

K

Y

Y

Q

K

R

Q

S

S

V

I

E

N

Q

D

P

P

E

D

A

T

F

F

W

W

A

G

D

E

I

Q

A

G

D

K

N

I

F

L

Q

D

W

W

K

I

K

T

K

P

W

Y

D

Q

K

K

V

V

L

K

D

N

W

T

N

S

F

F

K

A

-

P

-

G

E

N

P

V

K

S

I

I

E

K

W

W

F

Y

K

E

G

D

A

G

K

T

L

L

N

N

I

L

T

A

E

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

A

Q

D

E

K

N

G

G

D

D

Q

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T

A

A

I

I

W

W

E

E

A

G

N

D

D

D

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Q

H

S

H

K

R

H

V

I

L

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T

S

Y

Y

K

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Q

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L

L

H

H

E

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D

V

V

C

C

L

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F

F

A

A

N

N

V

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L

L

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L

N

D

N

L

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A

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A

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L

A

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C

A

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G

A

A

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N

A

A

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D

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L

L

F

F

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I

L

L

Y

Y

T
|
T

S

S

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G

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S

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G

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K

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P

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L

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F

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V

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A

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M

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V

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D

D

K

K

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L

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Y

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A

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L

L

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L

L

G

G

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S

V

V

G
|
G

E
|
E

P
|
P

I

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N

N

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P

E

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A

A

W

W

H

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W

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D

D

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|
T

W
|
W

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|
Q

T
|
T

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|
E

N

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G

G

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F

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M

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P

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A

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G

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L

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V

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D

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N

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G

G

A

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G

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L

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Y

F

F

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S

T

T

Y

F

E

K

D

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

C

A

L

R

R

R

D

D

E

E

D

D

G

G

Y

Y

R

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S

S

G

G

H

H

R
|
R

I

L

G

G

T

T

A

A

E

E

V

I

E

E

N

S

A

A

I

L

N

V

M

A

F

H

T

P

D

K

V

I

V

A

E

E

S

A

A

A

V

V

G

G

Y

I

P

P

H

H

E

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

I

T

L

L

D

N

K

H

E

G

S

E

E

E

D

P

V

S

E

P

L

E

T

L

K

Y

K

A

D

E

I

V

A

R

M

N

T

W

V

V

S

A

R

K

I

E

I

I

G

G

A

P

I

L

A

A

R

T

P

P

D

D

K

V

I

L

Q

H

F

W

V

T

T

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

G

G

D

D

M

T

K

S

N

N

V

-

G

-

D

D

V

T

S

S

T

T

L

L

L

A

D

D

P

P

A

G

V

V

E

E

E

K

I

L

K

E

G

E

K

K

active site: T260 (= T252), T412 (= T404), E413 (= E405), N517 (= N510), R522 (= R515), K605 (= K598)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G375), E384 (= E376), P385 (= P377), T408 (= T400), W409 (= W401), W410 (= W402), Q411 (= Q403), T412 (= T404), D496 (= D489), I508 (= I501), R511 (= R504), R522 (= R515)

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
54% identity, 99% coverage: 9:634/635 of query aligns to 18:638/640 of 5jrhA

query
sites
5jrhA

D

E

E

Q

Y

Y

K

E

E

T

A

K

Y

Y

Q

K

R

Q

S

S

V

I

E

N

Q

D

P

P

E

D

A

T

F

F

W

W

A

G

D

E

I

Q

A

G

D

K

N

I

F

L

Q

D

W

W

K

I

K

T

K

P

W

Y

D

Q

K

K

V

V

L

K

D

N

W

T

N

S

F

F

K

A

-

P

-

G

E

N

P

V

K

S

I

I

E

K

W

W

F

Y

K

E

G

D

A

G

K

T

L

L

N

N

I

L

T

A

E

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

A

Q

D

E

K

N

G

G

D

D

Q

R

P

T

A

A

I

I

W
|
W

E

E

A

G

N

D

D

D

P

T

N

S

E

Q

H

S

H

K

R

H

V

I

L

-

T

S

Y

Y

K

R

Q

E

L

L

H

H

E

R

K

D

V

V

C

C

L

R

F

F

A

A

N

N

V

T

L

L

K

L

N

D

N

L

D

G

V

I

K

K

G

G

D

D

R

V

V

V

C

A

I

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E
|
E

L

A

A

A

I

V

A

A

V

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

A

P

Q

E

S

A

I

V

A

A

D
x
G

R

R

I

I

N

I

D

D

A

S

Q

S

C

S

E

R

M

L

V

V

I

I

T

T

A

A

D

D

G

E

G

G

F

V

R
|
R

G

A

P

G

K

R

D

S

I

I

P

P

L

L

K

K

N

K

V

N

I

V

D

D

A

A

L

L

V

K

Q

N

-

P

-

N

C

V

P

T

S

S

V

V

K

E

T

H

V

V

I

I

V

V

L

L

T

K

R

R

T

T

R

G

T

S

P

D

I

I

S

D

M

W

I

Q

K

E

G

G

R

R

D

D

K

L

W

W

Q

R

D

D

E

L

I

I

H

E

K

K

V

A

E

S

T

P

L

-

G

-

M

-

I

-

D

E

C

H

P

Q

A

P

E

E

E

A

M

M

D

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

V

V

V

L

H

H

T

T

A

G

G

G

Y

Y

M

L

I

V

Y

Y

T

A

A

A

Y

T

T

T

F

F

Q

K

N

Y

V

V

F

F

Q

D

Y

Y

Q

H

P

P

Q

G

D

D

V

I

Y

Y

F

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

I

V

T

T

G

G

H

H

S

S

Y

Y

I

L

Y

Y

G

G

P

P

L

L

A

A

Q

C

G

G

A

A

T

T

L

L

M

M

F

F

E

E

G

G

V

V

P

P

T

N

Y

W

P

P

D

T

A

P

G

A

R

R

F

M

W

C

D

Q

I

V

V

V

D

D

K

K

F

H

K

Q

V

V

N

N

T

I

L

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

S

A

L

L

M

M

Q

A

S

E

G

G

L

D

D

K

Y

A

V

I

K

E

D

G

K

T

D

D

L

R

S

S

L

L

K

R

V

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

E

P

E

E

A

A

W

W

H

E

W

W

Y

Y

N

W

D

K

N

K

I

I

G

G

K

K

G

E

K

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

N

T

G

G

I

F

L

M

I

I

S

T

P

P

I

L

A

P

N

G

V

A

T

I

P

E

T

L

K

K

P

A

C

G

Y

S

A

A

T

T

L

R

P

P

L

F

P

F

G

G

V

V

Q

Q

P

P

V

A

L

L

V

V

D

D

E

N

N

E

G

G

A

H

V

P

I

Q

E

E

G

G

N

-

G

A

V

T

S

E

G

G

N

N

L

L

C

V

I

I

K

T

F

D

P

S

W

W

P

P

G

G

M

Q

L

A

R

R

T

T

T

L

Y

F

G

G

D

D

H

H

E

E

R

R

C

F

K

E

L

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

E

K

D

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

C

A

L

R

R

R

D

D

E

E

D

D

G

G

Y

Y

R

W

I
|
I

T

T

G

G

R

R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

I

L

G

G

T

T

A

A

E

E

V

I

E

E

N

S

A

A

I

L

N
x
V

M

A

F
x
H

T

P

D

K

V
x
I

V

A

E

E

S

A

A

A

V

V

G

G

Y

I

P

P

H

H

E

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

I

T

L

L

D

N

K

H

E

G

S

E

E

E

D

P

V

S

E

P

L

E

T

L

K

Y

K

A

D

E

I

V

A

R

M

N

T

W

V

V

S
x
R

R

K

I

E

I

I

G

G

A
x
P

I

L

A

A

R

T

P

P

D

D

K

V

I

L

Q

H

F

W

V

T

T

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

G

G

D

D

M

L

K

-

N

-

V

-

G

G

D

D

V

T

S

S

T

T

L

L

L

A

D

D

P

P

A

G

V

V

E

E

E

K

I

L

K

E

G

E

K

K

active site: T260 (= T252), T412 (= T404), E413 (= E405), N517 (= N510), R522 (= R515), K605 (= K598)
binding (r,r)-2,3-butanediol: W93 (= W82), E140 (= E130), G169 (≠ D159), K266 (= K258), P267 (= P259)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G375), E384 (= E376), P385 (= P377), T408 (= T400), W409 (= W401), W410 (= W402), Q411 (= Q403), T412 (= T404), D496 (= D489), I508 (= I501), N517 (= N510), R522 (= R515)
binding coenzyme a: F159 (= F149), G160 (= G150), G161 (= G151), R187 (= R177), S519 (= S512), R580 (≠ S573), P585 (≠ A578)
binding magnesium ion: V533 (≠ N526), H535 (≠ F528), I538 (≠ V531)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
54% identity, 99% coverage: 9:634/635 of query aligns to 17:635/637 of 2p2fA

query
sites
2p2fA

D

E

E

Q

Y

Y

K

E

E

T

A

K

Y

Y

Q

K

R

Q

S

S

V

I

E

N

Q

D

P

P

E

D

A

T

F

F

W

W

A

G

D

E

I

Q

A

G

D

K

N

I

F

L

Q

D

W

W

K

I

K

T

K

P

W

Y

D

Q

K

K

V

V

L

K

D

N

W

T

N

S

F

F

K

A

-

P

-

G

E

N

P

V

K

S

I

I

E

K

W

W

F

Y

K

E

G

D

A

G

K

T

L

L

N

N

I

L

T

A

E

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

A

Q

D

E

K

N

G

G

D

D

Q

R

P

T

A

A

I

I

W

W

E

E

A

G

N

D

D

D

P

T

N

S

E

Q

H

S

H

K

R

H

V

I

L

-

T

S

Y

Y

K

R

Q

E

L

L

H

H

E

R

K

D

V

V

C

C

L

R

F

F

A

A

N

N

V

T

L

L

K

L

N

D

N

L

D

G

V

I

K

K

G

G

D

D

R

V

V

V

C

A

I

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

L

A

A

A

I

V

A

A

V

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

G

G

F

F

S

S

A

P

Q

E

S

A

I

V

A

A

D

G

R

R

I

I

N

I

D

D

A

S

Q

S

C

S

E

R

M

L

V

V

I

I

T

T

A

A

D

D

G

E

G

G

F

V

R
|
R

G

A

P

G

K

R

D

S

I

I

P

P

L

L

K

K

N

K

V

N

I

V

D

D

A

A

L

L

V

K

Q

N

-

P

-

N

C

V

P

T

S

S

V

V

K

E

T

H

V

V

I

I

V

V

L

L

T

K

R

R

T

T

R

G

T

S

P

D

I

I

S

D

M

W

I

Q

K

E

G

G

R

R

D

D

K

L

W

W

Q

R

D

D

E

L

I

I

H

E

K

K

V

A

E

S

T

P

L

-

G

-

M

-

I

-

D

E

C

H

P

Q

A

P

E

E

E

A

M

M

D

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

A

G

G

G

Y

Y

M

L

I

V

Y

Y

T

A

A

A

Y

T

T

T

F

F

Q

K

N

Y

V

V

F

F

Q

D

Y

Y

Q

H

P

P

Q

G

D

D

V

I

Y

Y

F

W

C

C

T

T

A

A

D

D

I

V

G

G

W
|
W

I

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

Y

Y

G

G

P

P

L

L

A

A

Q

C

G

G

A

A

T

T

L

L

M

M

F

F

E

E

G

G

V

V

P
|
P

T

N

Y

W

P

P

D

T

A

P

G

A

R

R

F

M

W

C

D

Q

I

V

V

V

D

D

K

K

F

H

K

Q

V

V

N

N

T

I

L

L

Y

Y

T

T

A
|
A

P

P

T

T

A
|
A

I

I

R

R

S

A

L

L

M

M

Q

A

S

E

G

G

L

D

D

K

Y

A

V

I

K

E

D

G

K

T

D

D

L

R

S

S

L

L

K

R

V

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

E

P

E

E

A

A

W

W

H

E

W

W

Y

Y

N

W

D

K

N

K

I

I

G

G

K

K

G

E

K

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

N

T

G

G

I

F

L

M

I

I

S

T

P

P

I

L

A

P

N

G

V

A

T

I

P

E

T

L

K

K

P

A

C

G

Y

S

A

A

T

T

L

R

P

P

L

F

P

F

G

G

V

V

Q

Q

P

P

V

A

L

L

V

V

D

D

E

N

N

E

G

G

A

H

V

P

I

Q

E

E

G

G

N

-

G

A

V

T

S

E

G

G

N

N

L

L

C

V

I

I

K

T

F

D

P

S

W

W

P

P

G

G

M

Q

L

A

R

R

T

T

T

L

Y

F

G

G

D

D

H

H

E

E

R

R

C

F

K

E

L

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

E

K

D

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

C

A

L

R

R

R

D

D

E

E

D

D

G

G

Y

Y

R

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

I

L

G

G

T

T

A

A

E

E

V

I

E

E

N

S

A

A

I

L

N

V

M

A

F

H

T

P

D

K

V

I

V

A

E

E

S

A

A

A

V

V

G

G

Y

I

P

P

H

H

E

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

I

T

L

L

D

N

K

H

E

G

S

E

E

E

D

P

V

S

E

P

L

E

T

L

K

Y

K

A

D

E

I

V

A

R

M

N

T

W

V

V

S
x
R

R

K

I

E

I

I

G

G

A
x
P

I

L

A

A

R

T

P

P

D

D

K

V

I

L

Q

H

F

W

V

T

T

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

G

G

D

D

M

T

K

S

N

-

V

-

G

-

D

-

V

-

S

S

T

T

L

L

L

A

D

D

P

P

A

G

V

V

E

E

E

K

I

L

K

E

G

E

K

K

active site: T259 (= T252), T411 (= T404), E412 (= E405), N516 (= N510), R521 (= R515), K604 (= K598)
binding adenosine monophosphate: G382 (= G375), E383 (= E376), P384 (= P377), T407 (= T400), W408 (= W401), W409 (= W402), Q410 (= Q403), T411 (= T404), D495 (= D489), I507 (= I501), R510 (= R504), N516 (= N510), R521 (= R515)
binding coenzyme a: F158 (= F149), R186 (= R177), W304 (= W297), T306 (= T299), P329 (= P322), A352 (= A345), A355 (= A348), S518 (= S512), R579 (≠ S573), P584 (≠ A578)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
54% identity, 99% coverage: 9:634/635 of query aligns to 18:632/634 of 1pg3A

query
sites
1pg3A

D

E

E

Q

Y

Y

K

E

E

T

A

K

Y

Y

Q

K

R

Q

S

S

V

I

E

N

Q

D

P

P

E

D

A

T

F

F

W

W

A

G

D

E

I

Q

A

G

D

K

N

I

F

L

Q

D

W

W

K

I

K

T

K

P

W

Y

D

Q

K

K

V

V

L

K

D

N

W

T

N

S

F

F

K

A

-

P

-

G

E

N

P

V

K

S

I

I

E

K

W

W

F

Y

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|
K

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I

L

L

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K

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I

A

A

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A

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G

D

D

M

-

K

-

N

-

V

-

G

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D

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V

-

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-

T

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L

L

L

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V

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K

active site: T260 (= T252), T412 (= T404), E413 (= E405), N517 (= N510), R522 (= R515), K605 (= K598)
binding coenzyme a: F159 (= F149), G160 (= G150), R187 (= R177), R190 (≠ K180), A301 (= A293), T307 (= T299), P330 (= P322), A356 (= A348), S519 (= S512), R580 (≠ S573), P585 (≠ A578)
binding magnesium ion: V533 (≠ N526), H535 (≠ F528), I538 (≠ V531)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G375), E384 (= E376), P385 (= P377), T408 (= T400), W409 (= W401), W410 (= W402), Q411 (= Q403), T412 (= T404), D496 (= D489), R511 (= R504), R522 (= R515)

8u2rA Crystal structure of acetyl-coenzyme a synthetase from leishmania infantum (ethyl amp bound)
53% identity, 97% coverage: 15:631/635 of query aligns to 28:656/664 of 8u2rA

query
sites
8u2rA

Y

Y

Q

E

R

Y

S

S

V

I

E

E

Q

H

P

N

E

D

A

A

F

F

W

W

A

A

D

E

I

I

A

A

D

R

-

R

N

D

F

F

Q

Y

W

W

K

K

K

T

W

W

-

P

-

D

D

Q

K

H

V

V

L

K

D

S

W

Y

N

N

F

F

K

D

E

K

P

S

K

K

-

G

-

P

-

I

-

F

I

V

E

K

W

W

F

F

K

E

G

G

A

A

K

V

L

T

N

N

I

V

T

C

E

Y

N

N

C

A

I

L

D

D

R

R

H

H

L

L

A

P

D

A

K

H

G

K

D

D

Q

R

P

V

A

C

I

F

I

Y

W

F

E

E

A

G

N

N

D

D

P

P

N

S

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V

H

T

H

E

R

A

V

V

L

-

T

T

Y

Y

K

G

Q

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L

M

H

Y

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K

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V

V

C

V

L

E

F

L

A

A

N

N

V

V

L

L

K

K

N

Y

N

Q

-

Y

D

G

V

I

K

A

K

K

G

G

D

D

R

R

V

V

C

G

I

L

Y

Y

M

L

P

P

M

M

I

I

P

P