SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_017367863.1 NCBI__GCF_000691145.1:WP_017367863.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8rplB Amp-forming acetyl-coa synthetase from chloroflexota bacterium with bound acetyl amp (see paper)
41% identity, 97% coverage: 15:570/574 of query aligns to 53:630/630 of 8rplB

query
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8rplB

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binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] ethanoate: G391 (= G335), E392 (= E336), P393 (= P337), T416 (= T357), W417 (= W358), W418 (= W359), Q419 (≠ M360), T420 (= T361), D502 (= D442), R517 (= R457), K523 (≠ M463), R528 (= R468)
binding magnesium ion: V539 (= V479), H541 (= H481)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
39% identity, 96% coverage: 23:574/574 of query aligns to 55:631/648 of Q89WV5

query
sites
Q89WV5

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G263 (= G218) mutation to I: Loss of activity.
G266 (= G221) mutation to I: Great decrease in activity.
K269 (= K224) mutation to G: Great decrease in activity.
E414 (= E362) mutation to Q: Great decrease in activity.

P9WQD1 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
38% identity, 94% coverage: 36:574/574 of query aligns to 67:640/651 of P9WQD1

query
sites
P9WQD1

W

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L

G

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A

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F

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A

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A

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D

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A

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K

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D

D

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L

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R

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N

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D

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G

K
|
K

I

I

M

M

R

R

R

R

V

L

L

L

K

R

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D

W

V

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D

K617 (= K551) modified: N6-acetyllysine; mutation to R: Complete loss of acetyl-coenzyme A synthetase activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
38% identity, 97% coverage: 20:574/574 of query aligns to 53:628/652 of P27550

query
sites
P27550

Q

Q

A

K

Y

V

K

K

E

N

F

T

S

S

W

F

E

A

E

P

A

G

R

N

K

V

N

S

F

I

S

K

W

W

Y

Y

E

E

T

D

G

G

K

T

V

L

N

N

A

L

A

A

Y

A

E

N

A

C

I

L

D

D

R

R

H

H

-

L

A

Q

D

E

S

N

F

G

R

D

K

R

N

T

K

A

V

I

A

I

L

W

H

E

Y

G

K

D

D

D

S

A

Q

S

R

Q

D

S

E

K

K

H

Y

I

T

S

F

Y

R

K

D

E

M

L

K

H

I

R

K

D

T

V

N

C

K

R

A

F

G

A

N

N

L

T

L

L

R

L

E

E

Q

L

A

G

H

-

V

I

Q

K

K

K

G

G

D

D

R

V

V

V

F

A

I

I

F

Y

M

M

P

P

R

M

S

V

P

P

E

E

L

A

Y

A

F

V

L

A

L

M

L

L

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A

A

C

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A

K

R

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I

G

G

A

A

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F

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A

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F

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V

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A

D

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R

R

L

I

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I

N

D

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S

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N

A

S

K

R

V

L

I

V

V

I

T

T

T

S

P

D

D

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L

G

L

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E

R

-

A

-

G

-

R

R

S

I

I

P

P

F

L

A

K

E

K

-

N

-

V

-

D

-

D

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A

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-

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V

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K

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D

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Q

A

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M

D

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L

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S

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G

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T

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G

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K

P

P

K

K

G

G

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V

L

L

H

H

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T

H

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G

A

G

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M

L

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W

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G

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N

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M

A

L

L

M

M

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A

A

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D

D

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L

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G

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V

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A

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V

D

D

N

N

A

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G

N

N

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L

L

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L

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M

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G

N

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Q

S

T

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Y

F

F

M

S

P

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-

F

G

K

D

N

W

M

Y

Y

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S

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G

D

D

S

G

A

A

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R

M

R

D

D

E

E

D

D

G

G

Y

Y

F

Y

W

W

F

I

Q

T

G

G

R

R

V

V

D

D

D

D

V

V

I

L

M

N

T

V

S

S

G

G

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H

R

R

V

L

G

G

P

T

F

A

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V

I

E

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S

S

K

A

L

L

V

V

E

A

H

H

P

P

A

K

I

I

A

A

E

E

A

A

G

A

V

V

I

V

G

G

K

I

P

P

D

H

P

N

V

I

R

K

G

G

E

Q

I

A

I

I

K

Y

A

A

F

Y

I

V

A

T

L

L

R

N

S

H

G

G

Y

E

E

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P

P

T

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D

P

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L

L

K

Y

E

A

E

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I

V

R

R

T

N

F

W

V

V

K

R

K

K

G

E

L

I

A

G

A

P

H

L

A

A

A

T

P

P

R

D

E

V

I

L

E

H

F

W

K

T

D

D

K

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

R

R

V

I

L

L

K

R

A

-

W

-

E

-

L

-

N

K

L

I

P

A

A

A

G

G

D

D

L

T

S

S

T

N

M

L

E

G

D

D

K609 (= K551) modified: N6-acetyllysine; by autocatalysis

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
38% identity, 97% coverage: 20:574/574 of query aligns to 53:628/652 of Q8ZKF6

query
sites
Q8ZKF6

Q

Q

A

K

Y

V

K

K

E

N

F

T

S

S

W

F

E

A

E

P

A

G

R

N

K

V

N

S

F

I

S

K

W

W

Y

Y

E

E

T

D

G

G

K

T

V

L

N

N

A

L

A

A

Y

A

E

N

A

C

I

L

D

D

R

R

H

H

-

L

A

Q

D

E

S

N

F

G

R

D

K

R

N

T

K

A

V

I

A

I

L

W

H

E

Y

G

K

D

D

D

S

T

Q

S

R

Q

D

S

E

K

K

H

Y

I

T

S

F

Y

R

R

D

E

M

L

K

H

I

R

K

D

T

V

N

C

K

R

A

F

G

A

N

N

L

T

L

L

R

L

E

D

Q

L

A

G

H

-

V

I

Q

K

K

K

G

G

D

D

R

V

V

V

F

A

I

I

F

Y

M

M

P

P

R

M

S

V

P

P

E

E

L

A

Y

A

F

V

L

A

L

M

L

L

G

A

A

C

V

A

K

R

I

I

G

G

A

A

I

V

V

H

G

S

P

V

L

I

F

F

E

G

A

G

F

F

M

S

E

P

G

E

A

A

V

V

K

A

D

G

R

R

L

I

E

I

N

D

S

S

E

S

A

S

K

R

V

L

I

V

V

I

T

T

T

A

P

D

D

E

L

G

L

V

E

R

-

A

-

G

-
x
R

R

S

I

I

P

P

F

L

A

K

E

K

-

N

-

V

-

D

-

D

-

A

-

L

-

K

-

N

-

P

-

N

L

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P

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L

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V

V

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K

G

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D

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D

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W

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D

I

L

H

W

F

W

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R

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D

A

L

F

I

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E

Q

K

A

A

A

S

K

P

E

E

L

H

E

Q

I

P

E

E

W

A

M

M

D

N

E

A

K

E

D

D

G

P

Y

L

L

F

L

I

H

L

Y

Y

T

T

S

S

G

G

S

S

T

T

G

G

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K

P

P

K

K

G

G

V

V

L

L

H

H

V

T

H

T

G

G

A

G

-

Y

M

L

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V

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Y

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A

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A

Q

T

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T

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F

K

K

W

Y

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V

L

F

D

D

L

Y

K

H

E

P

E

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

P

V

G

G

W

W

V

V

T
|
T

G

G

T

H

V

S

Y

Y

G

L

I

L

F

Y

S

G

P

P

W

L

L

A

N

C

G

G

A

A

T

T

N

T

V

L

I

M

L

F

G

E

G

G

R

V

F

P

N
|
N

-

W

-

P

-

T

P

P

D

A

T

R

W

M

Y

C

E

Q

T

V

I

V

E

D

S

K

L

H

G

Q

V

V

T

N

V

I

W

L

Y

Y

S

T

A
|
A

P

P

T

T

A

A

F

I

R

R

M

A

L

L

M

M

G

A

A

E

G

G

D

D

D

K

L

A

I

I

Q

E

K

G

Y

T

D

D

L

R

S

S

S

S

L

L

R

R

H

I

V

L

L

G

S

S

V

V

G

G

E

E

P

P

L

I

N

N

P

P

E

E

V

A

I

W

R

E

W

W

G

Y

D

W

A

K

V

K

F

I

G

G

K

K

R

E

-

K

-

C

-

P

I

V

H

V

D

D

T

T

W

W

W

W

M

Q

T

T

E

E

T

T

G

G

A

G

Q

F

L

M

I

I

C

T

N

P

Y

L

P

P

-

G

C

A

M

I

D

E

I

L

K

K

P

A

G

G

S

S

M

A

G

T

K

R

P

P

I

F

P

F

G

G

V

V

E

Q

A

P

A

A

I

L

V

V

D

D

N

N

A

E

G

G

N

H

E

P

L

Q

P

E

P

G

Y

A

R

T

M

E

G

G

N

N

L

L

A

V

I

I

K

T

K

D

G

S

W

W

P

P

S

G

M

Q

M

A

H

R

T

T

I

L

W

F

N

G

N

D

P

H

E

E

K

R

Y

F

E

E

-

Q

S

T

Y

Y

F

F

M

S

P

T

-

F

G

K

D

N

W

M

Y

Y

V

F

S

S

G

G

D

D

S

G

A

A

Y

R

M

R

D

D

E

E

D

D

G

G

Y

Y

F

Y

W

W

F

I

Q

T

G

G

R

R

V

V

D
|
D

D

D

V

V

I

L

M

N

T

V

S
|
S

G
|
G

E

H

R
|
R

V

L

G

G

P

T

F

A

E

E

V

I

E

E

S

S

K

A

L

L

V

V

E

A

H

H

P

P

A

K

I

I

A

A

E

E

A

A

G

A

V

V

I

V

G

G

K

I

P

P

D

H

P

A

V

I

R

K

G

G

E

Q

I

A

I

I

K

Y

A

A

F

Y

I

V

A

T

L

L

R

N

S

H

G

G

Y

E

E

E

P

P

T

S

D

P

E

E

L

L

K

Y

E

A

E

E

I

V

R

R

T

N

F

W

V

V

K
x
R

K

K

G

E

L

I

A

G

A

P

H

L

A

A

A

T

P

P

R

D

E

V

I

L

E

H

F

W

K

T

D

D

K

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

R

R

V

I

L

L

K

R

A

-

W

-

E

-

L

-

N

K

L

I

P

A

A

A

G

G

D

D

L

T

S

S

T

N

M

L

E

G

D

D

R194 (vs. gap) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T262) binding CoA
N335 (= N286) binding CoA
A357 (= A305) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D459) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S465) binding CoA
G524 (= G466) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R468) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (≠ K526) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K551) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
37% identity, 97% coverage: 20:574/574 of query aligns to 49:628/641 of 2p20A

query
sites
2p20A

Q

Q

A

K

Y

V

K

K

E

N

F

T

S

S

W

F

E

A

E

P

A

G

R

N

K

V

N

S

F

I

S

K

W

W

Y

Y

E

E

T

D

G

G

K

T

V

L

N

N

A

L

A

A

Y

A

E

N

A

C

I

L

D

D

R

R

H

H

-

L

A

Q

D

E

S

N

F

G

R

D

K

R

N

T

K

A

V

I

A

I

L

W

H

E

Y

G

K

D

D

D

S

T

Q

S

R

Q

D

S

E

K

K

H

Y

I

T

S

F

Y

R

R

D

E

M

L

K

H

I

R

K

D

T

V

N

C

K

R

A

F

G

A

N

N

L

T

L

L

R

L

E

D

Q

L

A

G

H

-

V

I

Q

K

K

K

G

G

D

D

R

V

V

V

F

A

I

I

F

Y

M

M

P

P

R

M

S

V

P

P

E

E

L

A

Y

A

F

V

L

A

L

M

L

L

G

A

A

C

V

A

K

R

I

I

G

G

A

A

I

V

V

H

G

S

P

V

L

I

F

F

E

G

A

G

F

F

M

S

E

P

G

E

A

A

V

V

K

A

D

G

R

R

L

I

E

I

N

D

S

S

E

S

A

S

K

R

V

L

I

V

V

I

T

T

T

A

P

D

D

E

L

G

L

V

E

R

-

A

-

G

-

R

R

S

I

I

P

P

F

L

A

K

E

K

-

N

-

V

-

D

-

D

-

A

-

L

-

K

-

N

-

P

-

N

L

V

P

T

K

S

L

V

E

E

S

H

V

V

I

I

I

V

V

L

G

K

G

R

E

T

K

G

E

S

D

D

A

I

D

D

G

W

V

Q

-

E

-

G

R

R

T

D

I

L

H

W

F

W

E

R

E

D

A

L

F

I

E

E

Q

K

A

A

A

S

K

P

E

E

L

H

E

Q

I

P

E

E

W

A

M

M

D

N

E

A

K

E

D

D

G

P

Y

L

L

F

L

I

H

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

T

K

P

P

K

K

G

G

V

V

L

L

H

H

V

T

H

T

G

G

A

G

-

Y

M

L

V

V

Q

Y

Q

A

G

A

Q

T

T

T

G

F

K

K

W

Y

V

V

L

F

D

D

L

Y

K

H

E

P

E

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

P

V

G

G

W

W

V

V

T

T

G

G

T

H

V

S

Y

Y

G

L

I

L

F

Y

S

G

P

P

W

L

L

A

N

C

G

G

A

A

T

T

N

T

V

L

I

M

L

F

G

E

G

G

R

V

F

P

N

N

-

W

-

P

-

T

P

P

D

A

T

R

W

M

Y

C

E

Q

T

V

I

V

E

D

S

K

L

H

G

Q

V

V

T

N

V

I

W

L

Y

Y

S

T

A

A

P

P

T

T

A

A

F

I

R

R

M

A

L

L

M

M

G

A

A

E

G

G

D

D

D

K

L

A

I

I

Q

E

K

G

Y

T

D

D

L

R

S

S

S

S

L

L

R

R

H

I

V

L

L

G

S

S

V

V

G
|
G

E
|
E

P
|
P

L

I

N

N

P

P

E

E

V

A

I

W

R

E

W

W

G

Y

D

W

A

K

V

K

F

I

G

G

K

K

R

E

-

K

-

C

-

P

I

V

H

V

D

D

T
|
T

W
|
W

W
|
W

M
x
Q

T
|
T

E
|
E

T

T

G

G

A

G

Q

F

L

M

I

I

C

T

N

P

Y

L

P

P

-

G

C

A

M

I

D

E

I

L

K

K

P

A

G

G

S

S

M

A

G

T

K

R

P

P

I

F

P

F

G

G

V

V

E

Q

A

P

A

A

I

L

V

V

D

D

N

N

A

E

G

G

N

H

E

P

L

Q

P

E

P

G

Y

A

R

T

M

E

G

G

N

N

L

L

A

V

I

I

K

T

K

D

G

S

W

W

P

P

S

G

M

Q

M

A

H

R

T

T

I

L

W

F

N

G

N

D

P

H

E

E

K

R

Y

F

E

E

-

Q

S

T

Y

Y

F

F

M

S

P

T

-

F

G

K

D

N

W

M

Y

Y

V

F

S

S

G

G

D
|
D

S

G

A

A

Y

R

M

R

D

D

E

E

D

D

G

G

Y

Y

F

Y

W

W

F
x
I

Q

T

G

G

R
|
R

V

V

D

D

D

D

V

V

I

L

M
x
N

T

V

S

S

G

G

E

H

R
|
R

V

L

G

G

P

T

F

A

E

E

V

I

E

E

S

S

K

A

L

L

V

V

E

A

H

H

P

P

A

K

I

I

A

A

E

E

A

A

G

A

V

V

I

V

G

G

K

I

P

P

D

H

P

A

V

I

R

K

G

G

E

Q

I

A

I

I

K

Y

A

A

F

Y

I

V

A

T

L

L

R

N

S

H

G

G

Y

E

E

E

P

P

T

S

D

P

E

E

L

L

K

Y

E

A

E

E

I

V

R

R

T

N

F

W

V

V

K

A

K

K

G

E

L

I

A

G

A

P

H

L

A

A

A

T

P

P

R

D

E

V

I

L

E

H

F

W

K

T

D

D

K

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

R

R

V

I

L

L

K

R

A

K

W

I

E

A

L

A

N

G

L

D

P

T

A

S

G

N

D

D

L

T

S

S

T

T

M

L

E

A

D

D

active site: T260 (= T216), T412 (= T361), E413 (= E362), N517 (≠ M463), R522 (= R468), K605 (= K551)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G335), E384 (= E336), P385 (= P337), T408 (= T357), W409 (= W358), W410 (= W359), Q411 (≠ M360), T412 (= T361), D496 (= D442), I508 (≠ F454), R511 (= R457), R522 (= R468)

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
38% identity, 97% coverage: 20:574/574 of query aligns to 49:627/640 of 5jrhA

query
sites
5jrhA

Q

Q

A

K

Y

V

K

K

E

N

F

T

S

S

W

F

E

A

E

P

A

G

R

N

K

V

N

S

F

I

S

K

W

W

Y

Y

E

E

T

D

G

G

K

T

V

L

N

N

A

L

A

A

Y

A

E

N

A

C

I

L

D

D

R

R

H

H

-

L

A

Q

D

E

S

N

F

G

R

D

K

R

N

T

K

A

V

I

A

I

L
x
W

H

E

Y

G

K

D

D

D

S

T

Q

S

R

Q

D

S

E

K

K

H

Y

I

T

S

F

Y

R

R

D

E

M

L

K

H

I

R

K

D

T

V

N

C

K

R

A

F

G

A

N

N

L

T

L

L

R

L

E

D

Q

L

A

G

H

-

V

I

Q

K

K

K

G

G

D

D

R

V

V

V

F

A

I

I

F

Y

M

M

P

P

R

M

S

V

P

P

E
|
E

L

A

Y

A

F

V

L

A

L

M

L

L

G

A

A

C

V

A

K

R

I

I

G

G

A

A

I

V

V

H

G

S

P

V

L

I

F
|
F

E
x
G

A
x
G

F

F

M

S

E

P

G

E

A

A

V

V

K

A

D
x
G

R

R

L

I

E

I

N

D

S

S

E

S

A

S

K

R

V

L

I

V

V

I

T

T

T

A

P

D

D

E

L

G

L

V

E
x
R

-

A

-

G

-

R

R

S

I

I

P

P

F

L

A

K

E

K

-

N

-

V

-

D

-

D

-

A

-

L

-

K

-

N

-

P

-

N

L

V

P

T

K

S

L

V

E

E

S

H

V

V

I

I

I

V

V

L

G

K

G

R

E

T

K

G

E

S

D

D

A

I

D

D

G

W

V

Q

-

E

-

G

R

R

T

D

I

L

H

W

F

W

E

R

E

D

A

L

F

I

E

E

Q

K

A

A

A

S

K

P

E

E

L

H

E

Q

I

P

E

E

W

A

M

M

D

N

E

A

K

E

D

D

G

P

Y

L

L

F

L

I

H

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

T
x
K

P
|
P

K

K

G

G

V

V

L

L

H

H

V

T

H

T

G

G

A

G

-

Y

M

L

V

V

Q

Y

Q

A

G

A

Q

T

T

T

G

F

K

K

W

Y

V

V

L

F

D

D

L

Y

K

H

E

P

E

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

P

V

G

G

W

W

V

V

T

T

G

G

T

H

V

S

Y

Y

G

L

I

L

F

Y

S

G

P

P

W

L

L

A

N

C

G

G

A

A

T

T

N

T

V

L

I

M

L

F

G

E

G

G

R

V

F

P

N

N

-

W

-

P

-

T

P

P

D

A

T

R

W

M

Y

C

E

Q

T

V

I

V

E

D

S

K

L

H

G

Q

V

V

T

N

V

I

W

L

Y

Y

S

T

A

A

P

P

T

T

A

A

F

I

R

R

M

A

L

L

M

M

G

A

A

E

G

G

D

D

D

K

L

A

I

I

Q

E

K

G

Y

T

D

D

L

R

S

S

S

S

L

L

R

R

H

I

V

L

L

G

S

S

V

V

G
|
G

E
|
E

P
|
P

L

I

N

N

P

P

E

E

V

A

I

W

R

E

W

W

G

Y

D

W

A

K

V

K

F

I

G

G

K

K

R

E

-

K

-

C

-

P

I

V

H

V

D

D

T
|
T

W
|
W

W
|
W

M
x
Q

T
|
T

E
|
E

T

T

G

G

A

G

Q

F

L

M

I

I

C

T

N

P

Y

L

P

P

-

G

C

A

M

I

D

E

I

L

K

K

P

A

G

G

S

S

M

A

G

T

K

R

P

P

I

F

P

F

G

G

V

V

E

Q

A

P

A

A

I

L

V

V

D

D

N

N

A

E

G

G

N

H

E

P

L

Q

P

E

P

G

Y

A

R

T

M

E

G

G

N

N

L

L

A

V

I

I

K

T

K

D

G

S

W

W

P

P

S

G

M

Q

M

A

H

R

T

T

I

L

W

F

N

G

N

D

P

H

E

E

K

R

Y

F

E

E

-

Q

S

T

Y

Y

F

F

M

S

P

T

-

F

G

K

D

N

W

M

Y

Y

V

F

S

S

G

G

D
|
D

S

G

A

A

Y

R

M

R

D

D

E

E

D

D

G

G

Y

Y

F

Y

W

W

F
x
I

Q

T

G

G

R

R

V

V

D

D

D

D

V

V

I

L

M
x
N

T

V

S
|
S

G

G

E

H

R
|
R

V

L

G

G

P

T

F

A

E

E

V

I

E

E

S

S

K

A

L

L

V
|
V

E

A

H
|
H

P

P

A

K

I
|
I

A

A

E

E

A

A

G

A

V

V

I

V

G

G

K

I

P

P

D

H

P

A

V

I

R

K

G

G

E

Q

I

A

I

I

K

Y

A

A

F

Y

I

V

A

T

L

L

R

N

S

H

G

G

Y

E

E

E

P

P

T

S

D

P

E

E

L

L

K

Y

E

A

E

E

I

V

R

R

T

N

F

W

V

V

K
x
R

K

K

G

E

L

I

A

G

A
x
P

H

L

A

A

A

T

P

P

R

D

E

V

I

L

E

H

F

W

K

T

D

D

K

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

R

R

V

I

L

L

K

R

A

-

W

-

E

-

L

-

N

K

L

I

P

A

A

A

G

G

D

D

L

L

-

G

-

D

-

T

S

S

T

T

M

L

E

A

D

D

active site: T260 (= T216), T412 (= T361), E413 (= E362), N517 (≠ M463), R522 (= R468), K605 (= K551)
binding (r,r)-2,3-butanediol: W93 (≠ L63), E140 (= E111), G169 (≠ D140), K266 (≠ T222), P267 (= P223)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G335), E384 (= E336), P385 (= P337), T408 (= T357), W409 (= W358), W410 (= W359), Q411 (≠ M360), T412 (= T361), D496 (= D442), I508 (≠ F454), N517 (≠ M463), R522 (= R468)
binding coenzyme a: F159 (= F130), G160 (≠ E131), G161 (≠ A132), R187 (≠ E158), S519 (= S465), R580 (≠ K526), P585 (≠ A531)
binding magnesium ion: V533 (= V479), H535 (= H481), I538 (= I484)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
38% identity, 96% coverage: 20:571/574 of query aligns to 48:620/637 of 2p2fA

query
sites
2p2fA

Q

Q

A

K

Y

V

K

K

E

N

F

T

S

S

W

F

E

A

E

P

A

G

R

N

K

V

N

S

F

I

S

K

W

W

Y

Y

E

E

T

D

G

G

K

T

V

L

N

N

A

L

A

A

Y

A

E

N

A

C

I

L

D

D

R

R

H

H

-

L

A

Q

D

E

S

N

F

G

R

D

K

R

N

T

K

A

V

I

A

I

L

W

H

E

Y

G

K

D

D

D

S

T

Q

S

R

Q

D

S

E

K

K

H

Y

I

T

S

F

Y

R

R

D

E

M

L

K

H

I

R

K

D

T

V

N

C

K

R

A

F

G

A

N

N

L

T

L

L

R

L

E

D

Q

L

A

G

H

-

V

I

Q

K

K

K

G

G

D

D

R

V

V

V

F

A

I

I

F

Y

M

M

P

P

R

M

S

V

P

P

E

E

L

A

Y

A

F

V

L

A

L

M

L

L

G

A

A

C

V

A

K

R

I

I

G

G

A

A

I

V

V

H

G

S

P

V

L

I

F
|
F

E

G

A

G

F

F

M

S

E

P

G

E

A

A

V

V

K

A

D

G

R

R

L

I

E

I

N

D

S

S

E

S

A

S

K

R

V

L

I

V

V

I

T

T

T

A

P

D

D

E

L

G

L

V

E
x
R

-

A

-

G

-

R

R

S

I

I

P

P

F

L

A

K

E

K

-

N

-

V

-

D

-

D

-

A

-

L

-

K

-

N

-

P

-

N

L

V

P

T

K

S

L

V

E

E

S

H

V

V

I

I

I

V

V

L

G

K

G

R

E

T

K

G

E

S

D

D

A

I

D

D

G

W

V

Q

-

E

-

G

R

R

T

D

I

L

H

W

F

W

E

R

E

D

A

L

F

I

E

E

Q

K

A

A

A

S

K

P

E

E

L

H

E

Q

I

P

E

E

W

A

M

M

D

N

E

A

K

E

D

D

G

P

Y

L

L

F

L

I

H

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

T

K

P

P

K

K

G

G

V

V

L

L

H

H

V

T

H

T

G

G

A

G

-

Y

M

L

V

V

Q

Y

Q

A

G

A

Q

T

T

T

G

F

K

K

W

Y

V

V

L

F

D

D

L

Y

K

H

E

P

E

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

P

V

G

G

W
|
W

V

V

T
|
T

G

G

T

H

V

S

Y

Y

G

L

I

L

F

Y

S

G

P

P

W

L

L

A

N

C

G

G

A

A

T

T

N

T

V

L

I

M

L

F

G

E

G

G

R

V

F
x
P

N

N

-

W

-

P

-

T

P

P

D

A

T

R

W

M

Y

C

E

Q

T

V

I

V

E

D

S

K

L

H

G

Q

V

V

T

N

V

I

W

L

Y

Y

S

T

A
|
A

P

P

T

T

A
|
A

F

I

R

R

M

A

L

L

M

M

G

A

A

E

G

G

D

D

D

K

L

A

I

I

Q

E

K

G

Y

T

D

D

L

R

S

S

S

S

L

L

R

R

H

I

V

L

L

G

S

S

V

V

G
|
G

E
|
E

P
|
P

L

I

N

N

P

P

E

E

V

A

I

W

R

E

W

W

G

Y

D

W

A

K

V

K

F

I

G

G

K

K

R

E

-

K

-

C

-

P

I

V

H

V

D

D

T
|
T

W
|
W

W
|
W

M
x
Q

T
|
T

E
|
E

T

T

G

G

A

G

Q

F

L

M

I

I

C

T

N

P

Y

L

P

P

-

G

C

A

M

I

D

E

I

L

K

K

P

A

G

G

S

S

M

A

G

T

K

R

P

P

I

F

P

F

G

G

V

V

E

Q

A

P

A

A

I

L

V

V

D

D

N

N

A

E

G

G

N

H

E

P

L

Q

P

E

P

G

Y

A

R

T

M

E

G

G

N

N

L

L

A

V

I

I

K

T

K

D

G

S

W

W

P

P

S

G

M

Q

M

A

H

R

T

T

I

L

W

F

N

G

N

D

P

H

E

E

K

R

Y

F

E

E

-

Q

S

T

Y

Y

F

F

M

S

P

T

-

F

G

K

D

N

W

M

Y

Y

V

F

S

S

G

G

D
|
D

S

G

A

A

Y

R

M

R

D

D

E

E

D

D

G

G

Y

Y

F

Y

W

W

F
x
I

Q

T

G

G

R
|
R

V

V

D

D

D

D

V

V

I

L

M
x
N

T

V

S
|
S

G

G

E

H

R
|
R

V

L

G

G

P

T

F

A

E

E

V

I

E

E

S

S

K

A

L

L

V

V

E

A

H

H

P

P

A

K

I

I

A

A

E

E

A

A

G

A

V

V

I

V

G

G

K

I

P

P

D

H

P

A

V

I

R

K

G

G

E

Q

I

A

I

I

K

Y

A

A

F

Y

I

V

A

T

L

L

R

N

S

H

G

G

Y

E

E

E

P

P

T

S

D

P

E

E

L

L

K

Y

E

A

E

E

I

V

R

R

T

N

F

W

V

V

K
x
R

K

K

G

E

L

I

A

G

A
x
P

H

L

A

A

A

T

P

P

R

D

E

V

I

L

E

H

F

W

K

T

D

D

K

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I