SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_017505141.1 NCBI__GCF_001636925.1:WP_017505141.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
43% identity, 96% coverage: 5:192/195 of query aligns to 540:728/758 of O14289

query
sites
O14289
F
 
F
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T
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V
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E
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G
K
 
I
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A
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P
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P
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M
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A
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N
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D
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I
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N
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C
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F
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K
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M
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E
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Y
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A
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R
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E

Sites not aligning to the query:

P9WK95 3-isopropylmalate dehydratase small subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
39% identity, 95% coverage: 3:188/195 of query aligns to 2:184/198 of P9WK95

query
sites
P9WK95
E
 
E
P
 
A
F
 
F
Q
 
H
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H
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N
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E
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E

2pkpA Crystal structure of 3-isopropylmalate dehydratase (leud)from methhanocaldococcus jannaschii dsm2661 (mj1271) (see paper)
36% identity, 54% coverage: 19:123/195 of query aligns to 13:106/167 of 2pkpA

query
sites
2pkpA
N
x
D
I
 
V
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D
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A
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I
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I
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Y
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P
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Y
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E
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A
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S
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H
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C
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M
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A
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G
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I
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D
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E
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N
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G
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S
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S
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E
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A
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I
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Y
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R
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N
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N
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V
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G
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L
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I
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P
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I
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I
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A
S
 
N

Q58667 Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
36% identity, 54% coverage: 19:123/195 of query aligns to 13:106/170 of Q58667

query
sites
Q58667
N
 
D
I
 
V
D
 
D
T
 
T
D
 
D
Q
 
A
I
 
I
I
 
I
P
 
P
A
 
G
R
 
P
F
 
Y
L
 
L
T
x
R
T
|
T
T
 
T
T
 
D
R
 
P
E
 
Y
G
 
E
L
 
L
G
 
A
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S
-
 
H
-
 
C
A
 
M
A
 
A
F
 
G
Y
 
I
D
 
D
W
 
E
R
 
N
Y
 
F
E
 
-
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-
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-
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-
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-
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P
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K
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V
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-
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-
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-
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-
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-
P
 
-
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K
E
 
E
H
 
G
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D
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V
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I
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V
A
 
A
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G
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E
N
 
N
F
 
F
A
 
G
C
 
C
G
 
G
S
 
S
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S
R
 
R
E
 
E
H
 
Q
A
 
A
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V
W
 
I
A
 
A
L
 
I
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K
D
 
Y
Y
 
C
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G
F
 
I
R
 
K
A
 
A
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V
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I
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A
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K
E
 
S
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F
A
 
A
D
 
R
I
 
I
F
 
F
T
 
Y
S
 
R
N
 
N
A
 
A
L
 
I
K
 
N
N
 
V
G
 
G
L
 
L
L
 
I
P
 
P
I
 
I
V
 
I
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A
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N

Query Sequence

>WP_017505141.1 NCBI__GCF_001636925.1:WP_017505141.1
MSEPFQVLTSKTVTLSQANIDTDQIIPARFLTTTTREGLGKAAFYDWRYEDDGSEKPEAI
LNRIDPAEHRILLAGRNFACGSSREHAPWALLDYGFRAVISTEIADIFTSNALKNGLLPI
VVSQAVWDDLASQPDQPVTIDLQANQIQRGNAEPVPFGVESFARQCLLDGVDTLGWLQSN
MPEIEAYERSKETVR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory