SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_017505141.1 NCBI__GCF_001636925.1:WP_017505141.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
43% identity, 96% coverage: 5:192/195 of query aligns to 540:728/758 of O14289

query
sites
O14289

F

F

Q

T

V

V

L

V

T

E

S

G

K

I

T

A

V

A

T

P

L

L

S

P

Q

M

A

A

N

N

I

V

D

D

T

T

D

D

Q

K

I

I

P

P

A

K

R

Q

F

F

L

L

T

K

T

T

T

I

T

K

R

R

E

T

G

G

L

L

G

G

K

Q

A

F

A

A

F

F

Y

Y

D

E

W

I

R

R

Y

Y

E

D

D

A

D

D

G

G

S

K

E

E

K

I

P

P

E

D

A

F

I

V

L

L

N

N

R

R

I

-

D

E

P

P

A

Y

E

R

H

H

-

A

R

T

I

V

L

L

L

V

A

A

G

H

R

D

N

N

F

F

A

G

C

C

G

G

S

S

R

R

E

E

H

H

A

A

P

P

W

W

A

A

L

L

L

N

D

D

Y

F

G

G

F

I

R

R

A

V

V

I

I

I

S

A

T

P

E

S

I

F

A

A

D

D

I

I

F

F

T

F

S

N

N

N

A

C

L

F

K

K

N

N

G

G

L

M

L

L

P

P

I

I

V

P

V

T

S

P

Q

I

A

E

V

Q

W

V

D

N

D

D

L

M

A

M

S

K

Q

A

P

A

D

E

Q

N

P

Q

V

V

-

K

-

F

T

S

I

V

D

D

L

L

Q

V

A

N

N

Q

Q

T

I

I

Q

T

R

Y

G

G

N

D

A

K

E

Q

P

-

V

V

P

K

F

F

G

D

V

V

E

E

S

P

F

F

A

R

R

K

Q

H

C

C

L

L

L

V

D

N

G

G

V

L

D

D

T

D

L

I

G

G

W

L

L

T

Q

L

S

Q

N

K

M

E

P

T

E

M

I

I

E

D

A

A

Y

F

E

E

R

A

S

A

K

R

E

E

Sites not aligning to the query:

486 modified: Phosphoserine
488 modified: Phosphoserine

P9WK95 3-isopropylmalate dehydratase small subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
39% identity, 95% coverage: 3:188/195 of query aligns to 2:184/198 of P9WK95

query
sites
P9WK95

E

E

P

A

F

F

Q

H

V

T

L

H

T

S

S

G

K

I

T

G

V

V

T

P

L

L

S

R

Q

R

A

S

N

N

I

V

D

D

T

T

D

D

Q

Q

I

I

P

P

A

A

R

V

F

F

L

L

T

K

T

R

T

V

T

T

R

R

E

T

G

G

L

F

G

E

K

D

A

G

A

L

F

F

Y

A

D

G

W

W

R

R

Y

S

E

D

D

-

G

-

S

-

E

-

K

-

P

P

E

A

A

F

I

V

L

L

N

N

R

L

I

S

D

P

P

F

A

D

E

R

H

G

R

S

I

V

L

L

L

V

A

A

G

G

R

P

N

D

F

F

A

G

C

T

G

G

S

S

R

R

E

E

H

H

A

A

P

V

W

W

A

A

L

L

L

M

D

D

Y

Y

G

G

F

F

R

R

A

V

V

V

I

I

S

S

T

S

E

R

I

F

A

G

D

D

I

I

F

F

T

R

S

G

N

N

A

A

L

G

K

K

N

A

G

G

L

L

P

A

I

A

V

E

V

V

S

A

Q

Q

-

D

-

V

-

E

A

L

V

L

W

W

D

K

D

L

L

I

A

E

S

Q

Q

S

P

P

D

G

Q

L

P

E

V

I

T

T

I

A

D

N

L

L

Q

Q

A

-

N

D

Q

R

I

I

Q

I

R

T

G

A

N

A

A

T

E

V

P

V

V

L

P

P

F

F

G
x
K

V

I

E

D

S

D

F

H

A

S

R

A

Q

W

C

R

L

L

D

E

G

G

V

L

D

D

T

D

L

I

G

A

W

L

L

T

Q

L

S

R

N

K

M

L

P

D

E

E

I

I

E

E

A

A

Y

F

E

E

K154 (≠ G158) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

2pkpA Crystal structure of 3-isopropylmalate dehydratase (leud)from methhanocaldococcus jannaschii dsm2661 (mj1271) (see paper)
36% identity, 54% coverage: 19:123/195 of query aligns to 13:106/167 of 2pkpA

query
sites
2pkpA

N
x
D

I

V

D

D

T

T

D

D

Q

A

I

I

P

P

A

G

R

P

F

Y

L

L

T

R

T

T

T

D

R

P

E

Y

G

E

L

L

G

A

K

S

-

H

-

C

A

M

A

A

F

G

Y

I

D

D

W

E

R

N

Y

F

E

-

D

-

G

-

S

-

E

-

K

-

P

P

E

K

A

K

I

V

L

-

N

-

R

-

I

-

D

-

P

-

A

K

E

E

H

G

R

D

I

V

L

I

L

V

A

A

G

G

R

E

N

N

F

F

A

G

C
|
C

G

G

S

S

R

R

E

E

H

Q

A

A

P

V

W

I

A

A

L

I

L

K

D

Y

Y

C

G

G

F

I

R

K

A

A

V

V

I

I

S

A

T

K

E

S

I

F

A

A

D

R

I

I

F

F

T

Y

S

R

N

N

A

A

L

I

K

N

N

V

G

G

L

L

L

I

P

P

I

I

V

I

V

A

S

N

binding zinc ion: D13 (≠ N19), C63 (= C80)

Q58667 Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
36% identity, 54% coverage: 19:123/195 of query aligns to 13:106/170 of Q58667

query
sites
Q58667

N

D

I

V

D

D

T

T

D

D

Q

A

I

I

P

P

A

G

R

P

F

Y

L

L

T
x
R

T
|
T

T

T

T

D

R

P

E

Y

G

E

L

L

G

A

K

S

-

H

-

C

A

M

A

A

F

G

Y

I

D

D

W

E

R

N

Y

F

E

-

D

-

G

-

S

-

E

-

K

-

P

P

E

K

A

K

I

V

L

-

N

-

R

-

I

-

D

-

P

-

A

K

E

E

H

G

R

D

I

V

L

I

L

V

A

A

G

G

R

E

N

N

F

F

A

G

C

C

G

G

S

S

R

R

E

E

H

Q

A

A

P

V

W

I

A

A

L

I

L

K

D

Y

Y

C

G

G

F

I

R

K

A

A

V

V

I

I

S

A

T

K

E

S

I

F

A

A

D

R

I

I

F

F

T

Y

S

R

N

N

A

A

L

I

K

N

N

V

G

G

L

L

L

I

P

P

I

I

V

I

V

A

S

N

R26 (≠ T32) mutation R->K,V: Allows the recognition of substrates of IPMI enzymes since it becomes able to efficiently catalyze citraconate hydration and 3-isopropylmalate dehydration. Largely decreases substrate affinity for cis-homo(1-3)-aconitate.
T27 (= T33) mutation to A: Largely decreases substrate affinity for cis-homo(1-3)-aconitate while slightly increases activity on these substrates, and also decreases substrate affinity for maleate. Gains the ability to hydrate citraconate, an IPMI substrate.

Query Sequence

>WP_017505141.1 NCBI__GCF_001636925.1:WP_017505141.1
MSEPFQVLTSKTVTLSQANIDTDQIIPARFLTTTTREGLGKAAFYDWRYEDDGSEKPEAI
LNRIDPAEHRILLAGRNFACGSSREHAPWALLDYGFRAVISTEIADIFTSNALKNGLLPI
VVSQAVWDDLASQPDQPVTIDLQANQIQRGNAEPVPFGVESFARQCLLDGVDTLGWLQSN
MPEIEAYERSKETVR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory