SitesBLAST
Comparing WP_017537035.1 NCBI__GCF_000341205.1:WP_017537035.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 8 hits to proteins with known functional sites (download)
4lrzA Crystal structure of the e.Coli dhar(n)-dhal complex (see paper)
44% identity, 96% coverage: 9:206/207 of query aligns to 12:210/211 of 4lrzA
- binding adenosine-5'-diphosphate: D31 (= D28), D36 (= D33), D38 (= D35), H39 (= H36), G79 (= G76), A80 (= A77), S81 (= S78), L84 (= L81), G122 (= G118), T130 (= T126), M131 (≠ L127), G178 (= G174), D192 (= D188), P193 (= P189), G194 (= G190)
- binding magnesium ion: D31 (= D28), D36 (= D33), D36 (= D33), D38 (= D35), D38 (= D35)
P76014 PEP-dependent dihydroxyacetone kinase, ADP-binding subunit DhaL; EC 2.7.1.121 from Escherichia coli (strain K12) (see 3 papers)
44% identity, 94% coverage: 9:202/207 of query aligns to 11:205/210 of P76014
- D30 (= D28) binding Mg(2+)
- D35 (= D33) binding Mg(2+)
- D37 (= D35) binding Mg(2+)
- HGLN 38:41 (≠ HGTN 36:39) binding ADP
- AS 79:80 (= AS 77:78) binding ADP
- G121 (= G118) binding ADP
- M130 (≠ L127) binding ADP
- R178 (= R175) binding ADP
- DPG 191:193 (= DPG 188:190) binding ADP
Q9CIV7 PTS-dependent dihydroxyacetone kinase, ADP-binding subunit DhaL; EC 2.7.1.121 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see paper)
39% identity, 99% coverage: 1:205/207 of query aligns to 4:191/192 of Q9CIV7
- D29 (= D28) binding Mg(2+)
- D34 (= D33) binding Mg(2+)
- D36 (= D35) binding Mg(2+)
- HGAN 37:40 (≠ HGTN 36:39) binding ADP
- AS 78:79 (= AS 77:78) binding ADP
- R114 (≠ M117) mutation to A: Reduces activity 3-fold.; mutation to E: Reduces activity 100-fold.
- G115 (= G118) binding ADP
- M124 (≠ L127) binding ADP
- R161 (= R175) mutation to A: Loss of activity.
- Y164 (= Y178) mutation to A: Reduces activity about 20-fold.
- DPG 174:176 (= DPG 188:190) binding ADP
3cr3A Structure of a transient complex between dha-kinase subunits dham and dhal from lactococcus lactis (see paper)
39% identity, 99% coverage: 1:205/207 of query aligns to 4:191/192 of 3cr3A
- binding adenosine-5'-diphosphate: D29 (= D28), D34 (= D33), D36 (= D35), H37 (= H36), N40 (= N39), G77 (= G76), A78 (= A77), S79 (= S78), L82 (= L81), G115 (= G118), A117 (= A120), T123 (= T126), D174 (= D188), P175 (= P189), G176 (= G190)
- binding magnesium ion: D29 (= D28), D34 (= D33), D34 (= D33), D36 (= D35), D36 (= D35)
Q3LXA3 Triokinase/FMN cyclase; Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); EC 2.7.1.28; EC 2.7.1.29; EC 4.6.1.15 from Homo sapiens (Human) (see 4 papers)
39% identity, 82% coverage: 22:190/207 of query aligns to 390:558/575 of Q3LXA3
- D401 (= D33) mutation to A: Abolishes both kinase and FMN cyclase activities.
- D403 (= D35) mutation to A: Abolishes both kinase and FMN cyclase activities.
- C404 (≠ H36) mutation to A: Decreases both kinase and FMN cyclase activities.
- G445 (= G76) to S: in TKFCD; very severe decrease of triokinase and glycerone kinase activities; dbSNP:rs1590578831
- S446 (≠ A77) mutation to A: Decreases both kinase and FMN cyclase activities.
- R543 (= R175) to I: in TKFCD; reduced protein levels in patient cells; very severe decrease of triokinase and glycerone kinase activities; dbSNP:rs547013163
- D556 (= D188) mutation to A: Abolishes both kinase and FMN cyclase activities.
Sites not aligning to the query:
- 112 T→A: Highly decreases kinase activity. No effect on FMN cyclase activity.
- 185 A → T: in dbSNP:rs2260655
- 204 K→A: Slightly decreases kinase activity. No effect on FMN cyclase activity.
- 221 H→A: Abolishes kinase activity but not FMN cyclase activity.
P45510 Dihydroxyacetone kinase; DHA kinase; Glycerone kinase; EC 2.7.1.29 from Citrobacter freundii (see 2 papers)
35% identity, 98% coverage: 3:204/207 of query aligns to 355:549/552 of P45510
- D380 (= D28) mutation to A: Loss of kinase activity.
- D385 (= D33) mutation to A: Loss of kinase activity.
- DGDT 385:388 (≠ DGDH 33:36) binding ATP
- D387 (= D35) mutation to A: Loss of kinase activity.
- T388 (≠ H36) mutation to H: Reduced kinase activity.
- SS 431:432 (≠ AS 77:78) binding ATP
- G468 (= G118) binding ATP
- TM 476:477 (≠ TL 126:127) binding ATP
- DPG 533:535 (= DPG 188:190) binding ATP
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 220 active site, Tele-hemiaminal-histidine intermediate
1un9A Crystal structure of the dihydroxyacetone kinase from c. Freundii in complex with amp-pnp and mg2+ (see paper)
31% identity, 98% coverage: 3:204/207 of query aligns to 355:536/537 of 1un9A
- binding phosphoaminophosphonic acid-adenylate ester: D385 (= D33), D387 (= D35), T388 (≠ H36), T391 (≠ N39), G430 (= G76), S431 (≠ A77), S432 (= S78), L435 (= L81), G468 (= G118), A470 (= A120), T476 (= T126), M477 (≠ L127), D520 (= D188), G522 (= G190)
- binding magnesium ion: D380 (= D28), D385 (= D33), D385 (= D33), D387 (= D35), D387 (= D35)
Sites not aligning to the query:
7w7hA S suis faka-fakb2 complex structure (see paper)
32% identity, 61% coverage: 3:128/207 of query aligns to 5:131/541 of 7w7hA
Sites not aligning to the query:
Query Sequence
>WP_017537035.1 NCBI__GCF_000341205.1:WP_017537035.1
MDTALAKAWVEAAADAVSAGAEELSRLDAAIGDGDHGTNMDRGLTAARRAVADLEADGPG
PVLVKTGMTMISRVGGASGPLYGTLLRTAGKRLEGGEAGLADLAAALRAGLEAVQKMGAA
KPGDKTLVDALSPAVEALEKAAGQGAPLSEAAEAAAGAAEQGARATVPLQARKGRASYLG
ERSIGHEDPGAASSALLMRALADACRA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory